A fungicide composition containing sugar compounds and its application

Abstract

The invention discloses a fungicide composition containing a saccharide and its application, and belongs to the field of pesticide fungicides. The fungicide composition is composed of the following components in parts by weight: 0.1-6 parts by weight of metalaxyl, 0.2-24 parts by weight of oxadiazon, 0.5-5 parts by weight of saccharide, 1-10 parts by weight of wetting agent, and 55-98.2 parts by weight of dispersion medium. In the present invention, metalaxyl, oxadiazon and saccharide three act synergistically, not only with a good synergistic effect, but also effectively reducing the pollution to groundwater. The formula and process of the fungicide composition of the present invention are simple, there is no organic solvent and dust pollution, and the saccharide can also be used as an energy donor for crops to promote crop growth.

Description

Technical Field

[0001] The present invention relates to the field of pesticide fungicides, in particular to a fungicide composition containing a saccharide compound and application thereof. Background Art

[0002] Pesticides generally cannot be used directly. Usually, it is necessary to select appropriate auxiliary ingredients such as emulsifiers, stabilizers, dispersants, carriers, etc. based on the properties of the pesticide original and the application scenario. Through scientific and reasonable formulation processing technology, high-performance formulations such as soluble concentrates, emulsions in water, suspension concentrates, etc. are produced to improve the application defects of the pesticide original, improve efficacy, reduce toxicity, reduce pollution, avoid harm to beneficial organisms, delay the development of pest resistance, and thus expand the application range of pesticide varieties.

[0003] Metalaxyl, CAS number 57837-19-1, chemical name D,LN-(2,6-dimethylphenyl)-N-(2'-methoxyacetyl)alanine methyl ester, is stable in neutral and acidic media at room temperature, with hydrolysis DT50 values exceeding 200 days (pH = 1), 115 days (pH = 9), and 12 days (pH = 10). As a systemic fungicide, it has protective and therapeutic effects, being rapidly absorbed by plant roots, stems, and leaves and transported to various parts of the plant. It is used for stem and leaf treatment, seed treatment, and soil treatment, and is effective against diseases caused by downy mildew, phytophthora, and pythium.

[0004] Hymexazol, CAS number 10004-44-1, chemical name 5-methyl-1,2-oxazol-3-ol, is stable at room temperature. As a systemic fungicide and soil disinfectant, it binds to iron and aluminum ions in the soil, inhibiting spore germination. It is absorbed by plant roots and migrates within the root system. Metabolites in the plant enhance crop physiological activity, promoting plant growth and tillering. It is also effective against diseases caused by Pythium and Fusarium.

[0005] CN103190432A discloses a fungicide composition containing a mixture of three active ingredients: mefenamic acid, metalaxyl, and prochloraz, for preventing and controlling rice seedling blight and damping-off; CN112841190A discloses a coating agent for cruciferous vegetable seeds, and its preparation method and use method, wherein gibberellins, α-naphthylacetic acid, mefenamic acid, metalaxyl-M, and basic fuchsin are dissolved in water to prepare a liquid, which is then stirred and mixed with prepared hydrangea residue and methylcellulose to obtain the cruciferous vegetable seed coating agent. Although the above patents provide the formula and preparation method of the fungicide or seed coating agent, they only involve physical mixing of the active ingredients of the pesticide, and do not provide the synergistic mechanism of the composition. Summary of the Invention

[0006] In response to the deficiencies in the prior art, the present invention provides a fungicide composition containing a carbohydrate compound and its application, which forms a complex through multiple intermolecular interactions, improves the synergistic effect, and constructs a green and sustainable application method.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions.

[0008] In one aspect, the present invention provides a fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0009]

[0010] Furthermore, the mass ratio of metalaxyl to hymexazol is 1:(2-5).The mass ratio of the saccharide compound to the total mass of metalaxyl and hymexazol is 1.1-1.6:1.

[0011] Preferably, the carbohydrate compound is one or more of monosaccharides, disaccharides or polysaccharides, wherein monosaccharides generally refer to glucose, fructose, and galactose, disaccharides generally refer to sucrose and lactose, and polysaccharides generally refer to chitosan, polyfructose, and cellulose.

[0012] Preferably, the wetting agent is one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, sodium lignin sulfonate, and polyphosphate. The dispersion medium is water.

[0013] Preferably, the fungicide composition containing a carbohydrate compound is composed of the following components in parts by weight:

[0014]

[0015]

[0016] On the other hand, the present invention also provides an application method of the fungicide composition containing carbohydrate compounds, namely, spraying the stems and leaves to prevent and control seedling diseases in the early stage of the disease, or spraying the soil with water to disinfect the seedbed.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In the present invention, the molecular structures of metalaxyl and oxadiazole both have oxygen-containing groups. On the one hand, with sugar polyols, the water solubility of the product can be improved by weak interactions such as intermolecular hydrogen bonds and π-π. On the other hand, metalaxyl and oxadiazole have large water solubility, and their leaching effect is relatively strong. By interacting with sugar compounds, it is equivalent to grafting different sugar molecules on the surface of pesticide molecules, which increases the size of pesticide molecules to a certain extent, can effectively reduce the leaching effect of pesticides, and prevent pesticides from entering groundwater to form pollution. On the other hand, the addition of sugar compounds can increase the nutritional content of crops to a certain extent. In the present invention, metalaxyl, oxadiazole and sugar compounds work synergistically to effectively improve the bactericidal effect. DETAILED DESCRIPTION

[0019] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, a detailed description will be given below with reference to specific embodiments.

[0020] The materials and reagents used in the following examples can all be obtained from commercial sources.

[0021] The present invention provides a fungicide composition containing a polysaccharide compound and its application, and specific embodiments are as follows.

[0022] Example 1

[0023] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0024] 0.5 parts by weight of metalaxyl, 2.5 parts by weight of oxadiazon, 2.5 parts by weight of glucose, 1 part by weight of chitosan, 10 parts by weight of polyphosphate, and 83.5 parts by weight of water.

[0025] Example 2

[0026] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0027] 0.1 parts by weight of metalaxyl, 0.2 parts by weight of oxadiazon, 0.5 parts by weight of chitosan, 1 part by weight of sodium lauryl sulfate, and 98.2 parts by weight of water.

[0028] Example 3

[0029] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0030] 6 parts by weight of metalaxyl, 24 parts by weight of hymexazol, 5 parts by weight of sucrose, 10 parts by weight of sodium lignin sulfonate, and 55 parts by weight of water.

[0031] Example 4

[0032] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0033] 5 parts by weight of metalaxyl, 20 parts by weight of oxadiazon, 2.5 parts by weight of lactose, 10 parts by weight of polyphosphate, and 62.5 parts by weight of water.

[0034] Example 5

[0035] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0036] 0.5 parts by weight of metalaxyl, 2.5 parts by weight of oxadiazon, 3.5 parts by weight of glucose, 10 parts by weight of polyphosphate, and 84.5 parts by weight of water.

[0037] Example 6

[0038] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0039] 3 parts by weight of metalaxyl, 15 parts by weight of oxadipate, 4 parts by weight of fructose, 10 parts by weight of sodium dodecylbenzenesulfonate, and 68 parts by weight of water.

[0040] Example 7

[0041] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0042] 3 parts by weight of metalaxyl, 15 parts by weight of oxadiazon, 4 parts by weight of polyfructose, 10 parts by weight of sodium lignin sulfonate, and 68 parts by weight of water.

[0043] Example 8

[0044] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0045] 0.2 parts by weight of metalaxyl, 1 part by weight of oxadiazon, 1 part by weight of cellulose, 2 parts by weight of polyphosphate, and 95.8 parts by weight of water.

[0046] Example 9

[0047] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0048] 10 parts by weight of metalaxyl, 20 parts by weight of hymexazol, 4 parts by weight of galactose, 10 parts by weight of polyphosphate, and 56 parts by weight of water.

[0049] Example 10

[0050] A fungicide composition containing a carbohydrate compound, comprising the following components in parts by weight:

[0051] 0.5 parts by weight of metalaxyl, 2.5 parts by weight of oxazolidinone, 3.5 parts by weight of chitosan, 10 parts by weight of polyphosphate, and 83.5 parts by weight of water.

[0052] In order to further illustrate the beneficial effects of the present invention, a comparative example is constructed as follows.

[0053] Comparative Example 1

[0054] A fungicide composition, consisting of the following components in parts by weight:

[0055] 0.5 parts by weight of metalaxyl, 2.5 parts by weight of oxadiazon, 10 parts by weight of polyphosphate, and 87 parts by weight of water.

[0056] In accordance with HG / T 2467.7-2003 "Specifications for the Preparation of Pesticide Soluble Concentrates Product Standards", the appearance, pH value, water solubility, persistent foaming, heat storage stability, and low-temperature stability of Examples 1-10 and Comparative Example 1 were tested. The results are shown in Table 1.

[0057] Table 1 Determination of physicochemical properties of fungicide compositions

[0058]

[0059]

[0060] According to relevant standards, the physical and chemical indicators of Examples 1-10 and Comparative Example 1 above all meet the standards. In order to screen out a more excellent fungicide composition formula, the following method was used for further testing.

[0061] Surface Tension Measurement: The surface tension of the 1000-fold diluted solution was measured using a DCAT 21 surface tension meter using the hanging platinum plate method. Each measurement was performed by cleaning the platinum plate with anhydrous ethanol and ultrapure water, burning it with an alcohol burner until it glowed red, and then allowing it to stand at room temperature before measurement. Five measurements were performed and the average value was calculated. The experimental temperature was controlled at (298.0 ± 0.2) K, and the humidity was controlled at 65%.

[0062] Equilibrium contact angle measurement: Using an OCA 20 contact angle meter, a 2 μL droplet of a 1000-fold diluted solution was prepared using a microsyringe. The workbench was adjusted to allow the droplet to contact the PTFE membrane surface. The contact angle of the droplet on the PTFE membrane was calculated using SCA 20 software. The equilibrium contact angle was determined after 60 s, and 10 measurements were taken for each measurement, averaging the results. The experimental temperature was controlled at (298.0 ± 0.2) K, and the humidity was maintained at 65%.

[0063] Adhesion force was measured using a DCAT 21 surface tension meter. A 10 μL droplet of a 1000-fold diluted solution was placed on a metal ring using a microsyringe. The polytetrafluoroethylene membrane surface was then mounted on a lifting platform. As the platform gradually ascended, the solid surface approached the droplet. Once the two contacted, the platform gradually descended until the solid-liquid separation occurred. Adhesion force evolution was monitored by measuring the mass change using an inverted balance. Five measurements were performed and the average value was calculated. The experimental temperature was controlled at (298.0 ± 0.2) K, and the humidity was maintained at 65%.

[0064] Determination of deposition amount: Use WS-18D electric sprayer to spray 1000 times diluted liquid onto the surface of polytetrafluoroethylene membrane (inclination angle is 45°) by spraying. By measuring the mass change of polytetrafluoroethylene membrane before and after spraying, the deposition amount of liquid per unit area is calculated. The average value of 10 measurements is taken each time.

[0065] The above results are shown in Table 2.

[0066] Table 2 Determination of interfacial action parameters of fungicide compositions

[0067]

[0068]

[0069] As can be seen from Table 2, combined with the determination of relevant interfacial interaction parameters, the results show that the adhesion and deposition amounts of the bactericidal compositions prepared by adding different amounts of saccharide compounds are different; at the same time, the adhesion and deposition amounts of the bactericidal compositions prepared by adding different types of saccharide compounds are also quite different.

[0070] The inventors also conducted field efficacy experiments to study the control of rice damping-off disease. The specific steps included using a backpack sprayer to spray the crops in each plot with the fungicide compositions of Examples 1-10 and Comparative Example 1. A blank control group was sprayed with an equal amount of water at a fungicide application rate of 15 g / mu. Each fungicide was diluted 1000-fold before spraying. Disease incidence was investigated one day before and seven days after application. The specific experimental methods were conducted in accordance with the "Guidelines for Field Efficacy Tests of Pesticides." The control efficacy was calculated according to the formula, and the results are shown in Table 3.

[0071] Among them, rice damping-off disease is recorded in 6 levels based on the ratio of lesions to leaf area:

[0072] Level 0: no lesions;

[0073] Level 1: The lesion area accounts for less than 10-20% of the entire leaf area;

[0074] Level 2: The lesion area accounts for 21%-40% of the entire leaf area;

[0075] Level 3: The lesion area accounts for 41%-60% of the entire leaf area;

[0076] Level 4: The lesion area accounts for 61%-80% of the entire leaf area;

[0077] Level 5: The lesion area accounts for 81%-100% of the entire leaf area.

[0078] Based on the survey data, the disease index and prevention and control effect of each treatment were calculated.

[0079]

[0080]

[0081] Table 3 The control effect of each treatment on rice damping-off disease

[0082] Serial number Pre-drug disease index Disease index 7 days after medication Control effect (%) Example 1 19.95 8.53 80.06 Example 2 20.13 15.33 64.16 Example 3 19.41 9.20 78.48 Example 4 20.14 8.79 79.44 Example 5 20.07 12.57 70.60 Example 6 19.55 10.65 75.10 Example 7 19.87 11.77 72.48 Example 8 20.36 15.63 63.45 Example 9 19.95 14.05 67.15 Example 10 21.08 5.34 87.51 Comparative Example 1 20.28 13.92 67.45 control group 20.41 42.77 —

[0083] As shown in Table 3, the efficacy of the examples of the present invention was better than that of the control group. During the experiment, all the treatments were safe for rice and no phytotoxicity was observed. The rice in the examples of the present invention grew better.

[0084] In summary, the present invention not only has a high bactericidal effect through the synergistic effect of metalaxyl, oxazolidinone and sugar compounds, but also can serve as an energy donor for crops and promote crop growth.

[0085] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A fungicide composition containing a carbohydrate compound, characterized in that: The invention is composed of the following components in parts by weight: 0.5 parts by weight of metalaxyl, 2.5 parts by weight of oxazolidinone, 2.5 parts by weight of glucose, 1 part by weight of chitosan, 10 parts by weight of polyphosphate and 83.5 parts by weight of water.

2. The use of the fungicide composition containing a carbohydrate compound according to claim 1, characterized in that: The fungicide composition containing the sugar compound is used to prevent and control seedling diseases by spraying the stems and leaves at the early stage of the disease, or is added with water to spray the soil to disinfect the seedbed.

Images