A suspension containing avermectin and chlorantraniliprole and preparation method thereof

Abstract

The present invention relates to a suspension containing avermectin and chlorantraniliprole and a preparation method thereof, wherein the suspension comprises the following raw materials in percentage by weight: avermectin 0.5-3.5%; chlorantraniliprole 25%; emulsifying dispersant 48%; wetting agent 2-4%; anti-precipitation agent 0.15%; thixotropic agent 0.5%; antifreeze agent 5%; preservative 0.1%; water balance; wherein the avermectin is added in the form of avermectin fermentation liquid. Under the premise of ensuring the performance of various aspects of the preparation, the present invention directly adds avermectin in avermectin fermentation liquid, thereby greatly reducing the production cost.

Description

Technical Field

[0001] The invention relates to the technical field of biological pesticides, and in particular to a suspension containing avermectin and chlorantraniliprole and a preparation method thereof. Background Art

[0002] Avermectin, whose main active ingredients are avermectin B1 and avermectin B2, is a type of 16-membered macrolide compound with insecticidal, acaricidal and nematicidal activity first developed by Satoshi Omura of Kitasato University in Japan and Merck Company in the United States. It is a secondary metabolite produced by fermentation of Streptomyces avermitilis and mainly exists in the mycelium of Streptomyces avermitilis.

[0003] At present, due to the limitation of the preparation process, the mycelium of Streptomyces avermitilis is directly used to produce avermectin preparations, and the mycelium in the fermentation broth is easy to precipitate and block the spray pipe. The preparation production directly using avermectin fermentation broth not only has the problem of easy precipitation of mycelium, but also has the problem that the avermectin concentration is low, while the avermectin concentration used in the avermectin preparation is high, so it cannot be directly used. Therefore, the current avermectin is often extracted from the mycelium, and then prepared and sold on the market. The extraction process of avermectin is complicated, and a large amount of waste liquid and wastewater will be generated, which greatly increases the preparation cost. Therefore, it is very important to develop auxiliary materials and processes to prepare preparation dosage forms that directly use avermectin fermentation broth to reduce the production cost of avermectin. Summary of the invention

[0004] The object of the present invention is to overcome the defects of the prior art and provide a suspension containing avermectin and chlorantraniliprole and a preparation method thereof. Under the premise of ensuring the performance of the preparation in all aspects, avermectin is directly added to the fermentation broth of Streptomyces avermitilis, thereby greatly reducing the production cost.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0007]

[0008]

[0009] Wherein, the avermectin is added in the form of Streptomyces avermitilis fermentation broth.

[0010] Furthermore, the emulsifying dispersant is one or more of Emulson AG TRN14105, BX, polycarboxylates, alkylphenol polyoxyethylene formaldehyde condensates and their sulfates or phosphates, polycondensed naphthalene sulfonates, APE carboxylates, anionic sulfonates, calcium lignin sulfonate, sodium polynaphthalene formaldehyde sulfonate, alkylphenol polyoxyethylene phosphate, and sodium methylenebis(phthalate) sulfonate.

[0011] Furthermore, the sulfate of the alkylphenol polyoxyethylene formaldehyde condensate includes one or both of nonylphenol polyoxyethylene ether formaldehyde condensate sulfate sodium salt and nonylphenol polyoxyethylene ether formaldehyde condensate sulfate ammonium salt.

[0012] Furthermore, the wetting agent includes one or more of Emulson AGTRST, K12 series wetting agent, NPE series wetting agent, AEO series wetting agent, alkylphenol (or fatty alcohol) polyoxyethylene polyoxypropylene ether and its sulfate or phosphate, alkylphenol formaldehyde resin polyoxyethylene ether, alkylnaphthalene sulfonate, polyether modified polysiloxane, fatty alcohol polyoxyethylene ether, and opening powder.

[0013] Furthermore, the emulsifying dispersant is Emulson AG TRN14105, and the wetting agent is Emulson AGTRST; the mass ratio of Emulson AG TRN14105 to Emulson AGTRST is 2:1.

[0014] Furthermore, the anti-precipitation agent includes one or more of gum arabic, xanthan gum, and sodium alginate.

[0015] Furthermore, the antifreeze agent includes one or more of propylene glycol, ethylene glycol, glycerol or urea.

[0016] Furthermore, the thixotropic agent is magnesium aluminum silicate.

[0017] Furthermore, a suspension containing avermectin and chlorantraniliprole comprises the following raw materials in percentage by weight:

[0018]

[0019] Wherein, the avermectin B1 is added in the form of Streptomyces avermitilis fermentation broth.

[0020] Technical solution 2:

[0021] A method for preparing the suspension containing abamectin and chlorantraniliprole comprises the following steps:

[0022] Step 1, sterilizing the fermentation broth of Streptomyces avermitilis;

[0023] Step 2: After weighing the raw materials in proportion, add chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitating agent, thixotropic agent and antifreeze agent to the fermentation broth of Streptomyces avermitilis, add water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

[0024] Furthermore, before adding chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitating agent, thixotropic agent and antifreeze agent to the fermentation broth of Streptomyces avermitilis, the fermentation broth of Streptomyces avermitilis is first evaporated and concentrated until the content of avermectin in the fermentation broth is greater than 1.5 times the content of avermectin required in the suspension.

[0025] Furthermore, the sterilization is carried out at 115-125°C, 0.05-0.25 MPa for 20 minutes;

[0026] The temperature used for the evaporation concentration is 75° C. and the pressure is -0.1 MPa.

[0027] Compared with the prior art, the beneficial effects achieved by the present invention are as follows:

[0028] The invention reduces the amount of avermectin in the suspension concentrate by adding chlorantraniliprole as the second active ingredient, so that it is possible to add avermectin in the form of a Streptomyces avermitilis fermentation liquid. In addition, the invention also screens and optimizes auxiliary materials, so that it is possible to prepare the suspension concentrate by using the Streptomyces avermitilis fermentation liquid on the premise of meeting various performances of the suspension concentrate such as stability, dispersibility and suspension. The invention not only saves the extraction process of avermectin and greatly reduces the production cost, but also can use the nutrients in the Streptomyces avermitilis fermentation liquid as fertilizer to provide nutrition for crops, and the process is simple and easy to implement, and is easy to carry out large-scale production. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below with reference to the embodiments.

[0030] In the present invention,

[0031] 1. A fermentation broth of Streptomyces avermitilis, and a fermentation method thereof, comprising the following steps:

[0032] Step 1, seed fermentation culture: inoculate a single spore suspension of Streptomyces avermectinosum into a seed culture medium, and culture at 27-28° C. for 48-72 hours to obtain a seed solution;

[0033] Seed culture medium: weigh 0.82% yeast powder, 3.025% soluble starch, 0.51% sodium molybdate, 0.19% soy peptone, and 1.25% ammonium sulfate, add distilled water to make up, and adjust the pH to 7.

[0034] Step 2, fermentation culture: inoculate the seed liquid into the fermentation medium at a rate of 12% and incubate at 27-28°C.

[0035] After 8.5 days of cultivation, a fermentation liquid of Streptomyces avermitilis was obtained. The total content of avermectin in the fermentation liquid was 0.66% for avermectin B1 and 0.561% for avermectin B2. The total content of avermectin B1 and avermectin B2 in the fermentation liquid was 1.221%.

[0036] Fermentation medium: corn starch 12.5%, yeast powder 1.25%, CoCl2·6H2O 0.0052‰, MgSO4·7H2O 0.06%, CaCO 0.16%, polyethylene glycol 0.1%, add distilled water to make up, and adjust the pH to 7.

[0037] 2. Chlorantraniliprole technical drug (content 99.0%): purchased from Wuhu Wosheng Biotechnology Co., Ltd.;

[0038] 3. Emulson AG TRN14105: purchased from Lamberti Specialty Chemicals (Shanghai) Co., Ltd.;

[0039] 4. Emulson AGTRST: purchased from Lamberti Specialty Chemicals (Shanghai) Co., Ltd.;

[0040] 5. Alkylnaphthalene sulfonate: Hubei Kewode Chemical Co., Ltd.;

[0041] 6. Xanthan gum: purchased from Hebei Baipin Biotechnology Co., Ltd.;

[0042] 7. Magnesium aluminum silicate: purchased from Jinan Yongtai Chemical Co., Ltd.;

[0043] 8. Propylene glycol: purchased from Jinan Belle Chemical Co., Ltd.;

[0044] Example 1

[0045] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0046]

[0047] The avermectin B1 is added in the form of a fermentation broth of Streptomyces avermitilis;

[0048] In the fermentation broth of Streptomyces avermitilis, the ratio of avermectin B1 to avermectin B2 is 1:0.85; therefore, in the suspension, the percentage of avermectin B2 is 0.7225%;

[0049] A method for preparing a suspension containing avermectin and chlorantraniliprole comprises the following steps:

[0050] Step 1, sterilizing the fermentation liquid of Streptomyces avermitilis (avermectin B1 content of 0.66%, avermectin B2 content of 0.561%) at 115-125° C., 0.05-0.25 MPa for 20 min, and then evaporating and concentrating at 75° C., -0.1 MPa until the content of avermectin B1 is greater than or equal to 1.275%;

[0051] Step 2: After weighing the raw materials in proportion, add chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitant, thixotropic agent, antifreeze agent and preservative to the concentrated avermitilis fermentation broth, add water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

[0052] Example 2

[0053] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0054]

[0055] The avermectin B1 is added in the form of a fermentation broth of Streptomyces avermitilis;

[0056] In the fermentation broth of Streptomyces avermitilis, the ratio of avermectin B1 to avermectin B2 is 1:0.85; therefore, in the suspension, the percentage of avermectin B2 is 0.221%;

[0057] In the Streptomyces avermitilis fermentation broth, the ratio of avermectin B1 to avermectin B2 is 1:0.85;

[0058] A method for preparing a suspension containing avermectin and chlorantraniliprole comprises the following steps:

[0059] Step 1, sterilizing the fermentation liquid of Streptomyces avermitilis (avermectin B1 content is 0.66%, avermectin B2 content is 0.561%) at 115-125° C., 0.05-0.25 MPa for 20 min;

[0060] Step 2: After weighing the raw materials in proportion, add chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitant, thixotropic agent, antifreeze agent and preservative to the fermentation broth of Streptomyces avermitilis, add water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

[0061] Example 3

[0062] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0063]

[0064]

[0065] The avermectin B1 is added in the form of a fermentation broth of Streptomyces avermitilis;

[0066] In the fermentation broth of Streptomyces avermitilis, the ratio of avermectin B1 to avermectin B2 is 1:0.85; therefore, in the suspension, the percentage of avermectin B2 is 1.462%;

[0067] A method for preparing a suspension containing avermectin and chlorantraniliprole comprises the following steps:

[0068] Step 1, sterilizing the fermentation liquid of Streptomyces avermitilis (avermectin B1 content of 0.66%, avermectin B2 content of 0.561%) at 115-125° C. and 0.05-0.25 MPa for 20 min, and then evaporating and concentrating the fermentation liquid at 75° C. and -0.1 MPa until the content of avermectin B1 is greater than or equal to 2.58%;

[0069] Step 2: After weighing the raw materials in proportion, add chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitant, thixotropic agent, antifreeze agent and preservative to the concentrated avermitilis fermentation broth, add water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

[0070] Comparative Example 1

[0071] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0072]

[0073] The avermectin B1 is added in the form of a fermentation broth of Streptomyces avermitilis;

[0074] In the fermentation broth of Streptomyces avermitilis, the ratio of avermectin B1 to avermectin B2 is 1:0.85; therefore, in the suspension, the percentage of avermectin B2 is 0.7225%;

[0075] A method for preparing a suspension containing avermectin and chlorantraniliprole comprises the following steps: the same as Example 1.

[0076] Comparative Example 2

[0077] A suspension containing avermectin and chlorantraniliprole, comprising the following raw materials in percentage by weight:

[0078]

[0079] A method for preparing a suspension containing avermectin and chlorantraniliprole comprises the following steps:

[0080] After weighing the raw materials in proportion, add avermectin B1 technical, avermectin B2 technical, chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitating agent, thixotropic agent, antifreeze agent and preservative into water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

[0081] Effect example 1: Performance index determination

[0082] 50 g of the suspension prepared in each embodiment and comparative example were sampled and placed in a 100 ml screw-mouth glass bottle with a polytetrafluoroethylene seal for 7 days and then stored in a hot container for 14 days. The performance indexes were measured. The results are shown in Table 1.

[0083] Thermal storage stability: The degradation rate of avermectin B1 and the degradation rate of chlorfenapyr are determined and calculated according to the determination method in the national standard GBT19136-2021 Thermal storage stability of pesticides.

[0084] Suspension rate: According to the national standard GB / T 14825-2006

[0085] Table 1

[0086]

[0087] Effect example 2: Field test

[0088] Select 600m2 of cooperative fields of farmers in Guangxi Zhuang Autonomous Region Xingbai Pharmaceutical Group 2 , divided into 2 groups, 300m each 2 In the experiment, the crop planted was rice. The pesticide was used to control rice leaf folder. The pesticide was sprayed before the rice leaf folder reached the second instar stage, and the number of rice leaf folders was counted before spraying. The mortality of rice leaf folders was observed again on the 3rd and 6th days after spraying, and the mortality rate was calculated.

[0089] The five-point sampling method was used to survey and count the number of rice leaf folders. Each group surveyed 70 rice clumps, and the average number of live insects at each point was calculated to count the insect population base.

[0090] Test group: spray the suspension prepared in Example 1 of the present invention, the dosage per mu is 30g, and the spray is diluted with 35kg / mu of water;

[0091] Control group: spray the suspension prepared in comparative example 2 of the present invention, the dosage per mu is 30g, and the spray is diluted with 35kg / mu of water;

[0092] Table 2

[0093]

[0094] The above-described embodiments are only preferred embodiments of the present invention, and are not exhaustive of the feasible implementations of the present invention. For those skilled in the art, any obvious changes made thereto without departing from the principles and spirit of the present invention should be considered to be included in the scope of protection of the claims of the present invention.

Claims

1. A suspension concentrate containing avermectin and chlorantraniliprole, characterized in that: The following raw materials are included in weight percentage: Wherein, the avermectin is added in the form of a fermentation broth of Streptomyces avermitilis; The emulsifying dispersant is Emulson AG TRN14105; The wetting agent is Emulson AGTRST; The mass ratio of Emulson AG TRN14105 to Emulson AGTRST is 2:

1.

2. A suspension concentrate containing avermectin and chlorantraniliprole according to claim 1, characterized in that, The anti-precipitation agent includes one or more of gum arabic, xanthan gum and sodium alginate.

3. A suspension concentrate containing abamectin and chlorantraniliprole according to claim 1, characterized in that, The antifreeze agent includes one or more of propylene glycol, ethylene glycol, glycerol or urea.

4. A suspension concentrate containing avermectin and chlorantraniliprole according to claim 1, characterized in that, The thixotropic agent is magnesium aluminum silicate.

5. A suspension concentrate containing abamectin and chlorantraniliprole according to claim 1, characterized in that, The following raw materials are included in weight percentage: Wherein, the avermectin B1 is added in the form of Streptomyces avermitilis fermentation broth.

6. A method for preparing a suspension concentrate containing avermectin and chlorantraniliprole as claimed in any one of claims 1 to 5, characterized in that: The steps include: Step 1, sterilizing the fermentation broth of Streptomyces avermitilis; Step 2: After weighing the raw materials in proportion, add chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitating agent, thixotropic agent and antifreeze agent to the fermentation broth of Streptomyces avermitilis, add water, and grind to a particle size of less than or equal to 4.5 microns to obtain the product.

7. A method for preparing a suspension concentrate containing abamectin and chlorantraniliprole according to claim 6, characterized in that: Before adding chlorantraniliprole, emulsifying dispersant, wetting agent, anti-precipitating agent, thixotropic agent and antifreeze agent into the fermentation liquid of Streptomyces avermitilis, the fermentation liquid of Streptomyces avermitilis is firstly evaporated and concentrated until the content of avermectin in the fermentation liquid is greater than 1.5 times the content of avermectin required in the suspension.

8. A method for preparing a suspension concentrate containing abamectin and chlorantraniliprole according to claim 7, characterized in that: The sterilization is carried out at 115-125°C, 0.05-0.25 MPa for 20 minutes; The temperature used for the evaporation concentration is 75-85°C and the pressure is -0.1MPa.

Images