Composition for prolonging the ornamental period of colorful leaf osmanthus and its application

Abstract

The present invention belongs to the technical field of plant growth regulators, and particularly relates to a composition for prolonging the ornamental period of colorful leaf osmanthus and its application. The composition includes Composition I, and the raw materials of Composition I include ethephon, brassinolide, sucrose and Solvent A; the composition also includes Composition II, and the raw materials of Composition II include abscisic acid, methyl jasmonate, sucrose and Solvent B; Solvent B is the same as Solvent A. The colorful leaf osmanthus includes at least one of red series colorful leaf osmanthus varieties and yellow series colorful leaf osmanthus varieties; the yellow series colorful leaf osmanthus is sprayed with Composition I, and the red series colorful leaf osmanthus is sprayed with Composition I and Composition II. The composition in the present invention can make the expression time of its colorful leaves longer, prolong the ornamental time, and improve the ornamental value of the leaves of colorful leaf osmanthus.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and particularly relates to a composition for prolonging the viewing period of laurel wilfordii and an application thereof. Background Art

[0002] Coleus is a tree species with seasonal leaves that show color in spring and autumn. As the seasons change, the leaves gradually return to green. During this green transition, transitional colors such as orange, yellow, and white-green appear. However, the color expression of Coleus' leaves lasts for a relatively short time, generally only a few weeks to a month, and during this time, the color of the leaves may be suboptimal, such as not being bright enough or luster enough. This is mainly because the leaves of Coleus contain a large amount of pigments such as carotenoids and anthocyanins, which are greatly affected by factors such as temperature, light, and soil moisture, resulting in unstable changes in leaf color.

[0003] Analysis of pigments in the leaves of the red variety of Osmanthus fragrans at different color change stages indicates that the fundamental cause of leaf color variation in Osmanthus fragrans is differences in total chlorophyll and anthocyanin content. In particular, differences in anthocyanin content are the key pigments responsible for leaf color change. Osmanthus fragrans leaf cells contain three types of pigments: chlorophyll, anthocyanins, and carotenes. The relative concentrations of these pigments within the cells determine the leaf color of each variety. When the chlorophyll concentration is high, the leaves appear green; when the anthocyanin concentration is high, the leaves appear purple or red; and when the carotenoid concentration is high, the leaves appear orange or yellow. Sometimes these three types of pigments are unevenly distributed, sometimes concentrated at the leaf edges or in the center of the main vein, resulting in two- or three-toned leaves.

[0004] Chinese invention patent application CN108002916A discloses a composition for delaying the yellowing and falling of ginkgo leaves, and its use method. The composition comprises potassium dihydrogen phosphate, naphthaleneacetic acid, zinc diammonium humate, anti-falling agent, 2,4-dichloropropionic acid, magnesium sulfate, ferrous phosphate, copper phosphate, chelated manganese, chelated boron, and chelated molybdenum. The composition inhibits the formation of abscission layers at the petiole of ginkgo leaves, preventing them from yellowing and falling, extending their viewing period while also supplementing trace elements. However, the composition contains numerous raw materials and is relatively complex to prepare.

[0005] Chinese Patent Application for Invention CN1557140A discloses a preparation containing abscisic acid that can extend the flowering period. It is characterized by containing abscisic acid and a stabilizer, wherein the content of abscisic acid is 0.1 - 100 mg / L, the weight ratio of abscisic acid to the stabilizer is 1:1 - 1:5, and it also contains sucrose and a bactericide. This preparation can extend the flowering periods of field-grown flowers, potted flowers, and cut flowers. This flowering period extender not only can significantly extend the flowering period, but also has advantages such as a tiny dosage, cold and heat resistance, a wide range of flower spectra, and a long-lasting effect. However, this preparation acts on the flower body and is not applicable to leaves.

[0006] Chinese Patent Application for Invention CN106588302A The present invention discloses an inhibitor for extending the flowering period of ornamental plants and its preparation and usage methods. This inhibitor for extending the flowering period of ornamental plants is made from the following raw materials in parts by weight: 10 - 30 parts of maleic hydrazide, 3 - 5 parts of resveratrol, 2 - 5 parts of ginsenoside, 2 - 5 parts of a bactericide, 15 - 25 parts of nitrogen fertilizer, 5 - 10 parts of phosphate fertilizer, 5 - 10 parts of potassium fertilizer, and 0.5 - 1 part of a nano-carbon fertilizer synergist. The present invention first uses resveratrol and ginsenoside, which are used in anti-aging research, in an inhibitor for extending the flowering period of ornamental plants, and combines them with maleic hydrazide, a bactericide, and fertilizers. Each component plays a synergistic role, enabling it to extend plant senescence, enhance stress resistance, maintain a good growth condition of ornamental plants, and extend the ornamental period. However, this inhibitor requires many raw materials and has a high cost. At the same time, the effect of extending the ornamental period of leaf plants is not discussed.

[0007] Therefore, it is necessary to develop a composition that can extend the ornamental period of colored osmanthus to extend the duration of leaf color expression of colored osmanthus and simultaneously achieve the purpose of enhancing the leaf color. Summary of the Invention

[0008] In view of the above deficiencies in the prior art, the present invention provides a composition for extending the ornamental period of colored osmanthus and its application.

[0009] The present invention provides a composition for extending the ornamental period of colored osmanthus. The composition includes Composition I, and the raw materials of Composition I include ethephon, brassinolide, sucrose, and Solvent A.

[0010] Preferably, Solvent A is a mixed solution of Tween - 20, ethanol, and water.

[0011] Preferably, the mass concentration of Tween - 20 in the mixed solution is 0.01 - 0.03%, and the mass concentration of ethanol is 0.5 - 1.5%.

[0012] Preferably, the preparation method of Composition I includes: dissolving ethephon, brassinolide, and sucrose separately with Solvent A to prepare an ethephon solution, a brassinolide solution, and a sucrose solution, and then mixing them to obtain it.

[0013] Preferably, the mass concentrations of the ethephon solution, brassinolide solution and sucrose solution are 100 - 300 mg / L, 0.2 - 0.6 mg / L and 5 - 15 g / L respectively; the volume ratio of the mixture is ethephon solution:brassinolide solution:sucrose solution = 1:0.8 - 1.2:0.8 - 1.2, preferably 1:1:1.

[0014] Preferably, the composition further comprises Composition II, and the raw materials of Composition II include abscisic acid, methyl jasmonate, sucrose and Solvent B; Solvent B is the same as Solvent A.

[0015] Preferably, the preparation method of Composition II comprises: dissolving abscisic acid, methyl jasmonate and sucrose respectively with Solvent B to prepare an abscisic acid solution, a methyl jasmonate solution and a sucrose solution, and then mixing them to obtain the composition.

[0016] Preferably, the mass concentrations of the abscisic acid solution, methyl jasmonate solution and sucrose solution are 50 - 150 mg / L, 10 - 50 mg / L and 5 - 15 g / L respectively; the volume ratio of the mixture is abscisic acid solution:methyl jasmonate solution:sucrose solution = 1:0.8 - 1.2:0.8 - 1.2, preferably 1:1:1.

[0017] The present invention also relates to the application of the above composition in prolonging the ornamental period of colored-leaf Osmanthus fragrans, and the colored-leaf Osmanthus fragrans includes at least one of red-colored-leaf Osmanthus fragrans varieties and yellow-colored-leaf Osmanthus fragrans varieties; the yellow-colored-leaf Osmanthus fragrans is sprayed with Composition I, and the red-colored-leaf Osmanthus fragrans is sprayed with Composition I and Composition II.

[0018] Preferably, the yellow-colored-leaf Osmanthus fragrans is sprayed with Composition I 4 - 6 days after the new leaves germinate, and Composition I is repeatedly sprayed 10 - 15 days later; the red-colored-leaf Osmanthus fragrans is sprayed with Composition II 4 - 6 days after the new leaves germinate, and Composition II is repeatedly sprayed 10 - 15 days later. 3 - 5 days after the leaves turn yellow, Composition I is sprayed.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) By spraying different compositions at intervals for different-colored-leaf Osmanthus fragrans, without affecting the normal growth of colored-leaf Osmanthus fragrans, the colored-leaf period of colored-leaf Osmanthus fragrans is prolonged by more than 20 days;

[0021] (2) It can make the color change of colored-leaf Osmanthus fragrans more uniform and pure, improve the stability of leaf color change, enhance the leaf color, and has a good landscape effect;

[0022] (3) A synergistic effect is produced between ethephon and brassinolide in Composition I; a synergistic effect is produced between abscisic acid and methyl jasmonate in Composition II, both of which can further prolong the ornamental period;

[0023] (4) The operation of the present invention is simple, and the spraying method is convenient and easy to operate. Detailed implementation manners

[0024] The technical solutions in the embodiments of the present invention are further clearly described. The described embodiments are only a part of the present invention, which is used to explain the present invention but not to limit the present invention. Therefore, other embodiments obtained by other technical personnel in the field without creative labor belong to the protection scope of the present invention.

[0025] Reagents not specifically described in the following embodiments are all conventional reagents in the art and can be obtained commercially; all the colorful leaf cinnamon trees grow in the same area, with the same growth conditions, and the external influencing factors such as sunlight and humidity are all the same. The growth temperature is controlled at 25 - 30 °C and the humidity is 75%. In each of the following examples and comparative examples, 10 colorful leaf cinnamon trees are used as the research objects, and the average value is obtained by recording the number of days.

[0026] Example 1

[0027] 1. Composition for prolonging the ornamental period of colorful leaf cinnamon tree

[0028] Use a mixed solution containing Tween-20, ethanol and water (the mass concentration of Tween-2 is 0.02%, and the mass concentration of ethanol is 1%) as the solvent to prepare the following solutions;

[0029] Composition I: A solution of 200 mg / L ethephon, a solution of 0.4 mg / L brassinolide and a solution of 10 g / L sucrose, which are mixed evenly according to the volume ratio of 1:1:1;

[0030] Composition II: A solution of 100 mg / L abscisic acid, a solution of 30 mg / L methyl jasmonate and a solution of 10 g / L sucrose, which are mixed evenly according to the volume ratio of 1:1:1.

[0031] 2. Application of the composition:

[0032] For the yellow-colored colorful leaf cinnamon tree variety (‘Luocai No. 16’): Select 10 colorful leaf cinnamon trees that have grown for 3 years, are growing vigorously and have similar growth trends as the experimental objects. Five days after the emergence of the yellow new leaves, use Composition I and spray it on the color-changing young leaves of the above-mentioned colorful leaf cinnamon trees; when spraying, spray evenly on the leaves until all are wet. After 15 days, repeat the operation and spray Composition I, and record the number of days of the yellow ornamental period.

[0033] Red-leafed osmanthus variety (‘Luocai No. 2’): Select 10 osmanthus plants with 3-year growth, strong growth and similar growth vigor as experimental objects. Five days after the emergence of new red leaves, use Composition II and spray it on the color-changing young leaves of the above osmanthus; when spraying, spray evenly on the leaves until all are wet. After 15 days, repeat the operation of spraying Composition II and record the number of days of the red ornamental period; three days after the leaves turn yellow, spray Composition I to extend the ornamental period of the yellow leaves and record the number of days of the yellow ornamental period.

[0034] Example 2

[0035] 1. Composition for extending the ornamental period of osmanthus

[0036] Prepare the following solutions using a mixed solution containing Tween-20, ethanol and water (the mass concentration of Tween-2 is 0.01%, and the mass concentration of ethanol is 1.5%) as the solvent;

[0037] Composition I: A solution of 100 mg / L ethephon, a solution of 0.2 mg / L brassinolide and a solution of 5 g / L sucrose, mixed evenly in a volume ratio of 1:1:1;

[0038] Composition II: A solution of 50 mg / L abscisic acid, a solution of 10 mg / L methyl jasmonate and a solution of 5 g / L sucrose, mixed evenly in a volume ratio of 1:1:1.

[0039] 2. Application of the composition:

[0040] Yellow-leafed osmanthus variety (‘Luocai No. 17’): Select 10 osmanthus plants with 3-year growth, strong growth and similar growth vigor as experimental objects. Six days after the emergence of new yellow leaves, use Composition I and spray it on the color-changing young leaves of the above osmanthus; when spraying, spray evenly on the leaves until all are wet. After 15 days, repeat the operation of spraying Composition II and record the number of days of the yellow ornamental period.

[0041] Red-leafed osmanthus variety (‘Luocai No. 19’): Select 10 osmanthus plants with 3-year growth, strong growth and similar growth vigor as experimental objects. Six days after the emergence of new red leaves, use Composition II and spray it on the color-changing young leaves of the above osmanthus; when spraying, spray evenly on the leaves until all are wet. After 13 days, repeat the operation of spraying Composition II and record the number of days of the red ornamental period; five days after the leaves turn yellow, spray Composition I to extend the ornamental period of the yellow leaves and record the number of days of the yellow ornamental period.

[0042] Example 3

[0043] 1. Composition for extending the ornamental period of osmanthus

[0044] The following solution was prepared using a mixture of Tween-20, ethanol, and water (Tween-2 concentration was 0.03% and ethanol concentration was 0.5%) as a solvent;

[0045] Composition I: 300 mg / L ethephon solution, 0.6 mg / L brassinolide solution, and 15 g / L sucrose solution, mixed uniformly in a volume ratio of 1:1:1;

[0046] Composition II: 150 mg / L abscisic acid solution, 50 mg / L methyl jasmonate solution and 15 g / L sucrose solution are uniformly mixed in a volume ratio of 1:1:1.

[0047] 2. Application of the composition:

[0048] Yellow-colored coleus variety ('Mincai 13'): Ten three-year-old, vigorous, and similarly growing coleus plants were selected as experimental subjects. Four days after the emergence of new yellow leaves, Composition I was sprayed on the discolored young leaves of the coleus. The spray was evenly applied to the leaves, wetting them completely. After 10 days, the spraying was repeated, and the number of days of the yellow viewing period was recorded.

[0049] Red-colored coleus varieties ('Yuntian Colored Osmanthus'): 10 coleus cultivars of 3 years old, vigorous growth, and similar growth vigour were selected as experimental subjects. Four days after the emergence of new red leaves, Composition II was sprayed on the young, discolored leaves of the coleus. The spraying was evenly applied to the leaves until all were wet. After 10 days, the spraying operation was repeated with Composition II, and the number of days of red viewing period was recorded. Three days after the leaves turned yellow, Composition I was sprayed to extend the viewing period of the yellow leaves, and the number of days of yellow viewing period was recorded.

[0050] Comparative Example 1

[0051] 1. Composition for prolonging the viewing period of colorful osmanthus:

[0052] The following solution was prepared using a mixture of Tween-20, ethanol, and water (Tween-2 concentration was 0.02% and ethanol concentration was 1%) as a solvent;

[0053] Composition I: 200 mg / L ethephon solution, 0.4 mg / L brassinolide solution, and 10 g / L sucrose solution, mixed uniformly in a volume ratio of 1:1:1;

[0054] Composition II: 100 mg / L abscisic acid solution, 30 mg / L abscisic acid solution and 10 g / L sucrose solution are mixed uniformly in a volume ratio of 1:1:1.

[0055] 2. The application of the composition is exactly the same as in Example 1.

[0056] Comparative Example 2

[0057] 1. Composition for prolonging the ornamental period of colorful leaf cinnamon:

[0058] Prepare the following solutions using a mixed solution containing Tween-20, ethanol and water (the mass concentration of Tween-2 is 0.02%, and the mass concentration of ethanol is 1%) as the solvent;

[0059] Composition I: A solution of ethephon at 200 mg / L, a solution of brassinolide at 0.4 mg / L, and a sucrose solution at 10 g / L are mixed evenly in a volume ratio of 1:1:1 to obtain;

[0060] Composition II: A solution of methyl jasmonate at 100 mg / L, a solution of methyl jasmonate at 30 mg / L, and a sucrose solution at 10 g / L are mixed evenly in a volume ratio of 1:1:1 to obtain.

[0061] 2. The application of the composition is exactly the same as that in Example 1.

[0062] Comparative Example 3

[0063] 1. Composition for prolonging the ornamental period of colorful leaf cinnamon:

[0064] Prepare the following solutions using a mixed solution containing Tween-20, ethanol and water (the mass concentration of Tween-2 is 0.02%, and the mass concentration of ethanol is 1%) as the solvent;

[0065] Composition I: A solution of ethephon at 200 mg / L, a solution of ethephon at 0.4 mg / L, and a sucrose solution at 10 g / L are mixed evenly in a volume ratio of 1:1:1 to obtain;

[0066] Composition II: A solution of abscisic acid at 100 mg / L, a solution of methyl jasmonate at 30 mg / L, and a sucrose solution at 10 g / L are mixed evenly in a volume ratio of 1:1:1 to obtain.

[0067] 2. The application of the composition is exactly the same as that in Example 1.

[0068] Comparative Example 4

[0069] 1. Composition for prolonging the ornamental period of colorful leaf cinnamon:

[0070] Prepare the following solutions using a mixed solution containing Tween-20, ethanol and water (the mass concentration of Tween-2 is 0.02%, and the mass concentration of ethanol is 1%) as the solvent;

[0071] Composition I: A solution of brassinolide at 200 mg / L, a solution of brassinolide at 0.4 mg / L, and a sucrose solution at 10 g / L are mixed evenly in a volume ratio of 1:1:1 to obtain;

[0072] Composition II: Prepared by uniformly mixing an abscisic acid solution with a concentration of 100 mg / L, a methyl jasmonate solution with a concentration of 30 mg / L, and a sucrose solution with a concentration of 10 g / L in a volume ratio of 1:1:1.

[0073] 2. The application of the composition is completely the same as that in Example 1.

[0074] Comparative Example 5

[0075] 1. Composition for extending the ornamental period of colored-leaf osmanthus

[0076] Prepare the following solutions using a mixed solution containing Tween-20, ethanol, and water (the mass concentration of Tween-2 is 0.02%, and the mass concentration of ethanol is 1%) as the solvent;

[0077] Composition I: Prepared by uniformly mixing an ethephon solution with a concentration of 200 mg / L, a citric acid solution with a concentration of 2 g / L, and a sucrose solution with a concentration of 10 g / L in a volume ratio of 1:1:1;

[0078] Composition II: Prepared by uniformly mixing an abscisic acid solution with a concentration of 100 mg / L, a gibberellin solution with a concentration of 2 mg / L, and a sucrose solution with a concentration of 10 g / L in a volume ratio of 1:1:1.

[0079] 2. The application of the composition is completely the same as that in Example 1.

[0080] The present invention also uses a 1% ethanol aqueous solution containing 0.02% Tween-20 and a commercially available retardant daminozide (Wuhan Haorong, 1596-84-5) as a control group, and sprays colored-leaf osmanthus of red and yellow series. The spraying method and time are completely the same as those in Example 1. The effect test is shown in Table 1, and some applicable colored-leaf osmanthus varieties are shown in Table 2.

[0081] Table 1 Test results of the ornamental period

[0082]

[0083]

[0084] Table 2 Some applicable colored-leaf osmanthus varieties

[0085] Red series Variety name Yellow series Variety name 1 ‘Luocai No. 2’ 1 ‘Luocai No. 16’ 2 ‘Luocai No. 19’ 2 ‘Luocai No. 17’ 3 ‘Yuntian Caigui’ 3 ‘Mincai 13’ 4 ‘Qiannan Guifei’ 4 ‘Mincai 25’ 5 ‘Zi Yan Gongzhu’ 5 ‘Mincai 28’ 6 ‘Yongfu Zixuan’ 6 ‘Yongfu Xuancai’ 7 ‘Minnong Guiguan’ 7 ‘Chaoyang Jinzuan’ 8 ‘Nanlin Caiyun’ 8 ‘Jinyu Guihua’

[0086] The results show that compared with the blank control, Examples 1-3 can significantly extend the ornamental period of colored-leaf osmanthus, with an average extension of more than 20 days; the commercially available retardant daminozide can increase chlorophyll synthesis and shorten the ornamental period after spraying; the extension effects of Comparative Examples 1-5 are inferior to those of Examples 1-3. Therefore, Composition I can extend the ornamental period of yellow-colored-leaf osmanthus; Composition I and Composition II can extend the ornamental period of red-colored-leaf osmanthus; the compositions provided by the present invention can enhance the leaf color and improve the stability of leaf color change at the same time.

[0087] The above detailed description is a specific description of one feasible embodiment of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change made without departing from the present invention shall be included within the scope of the technical solution of the present invention.

Claims

1. A composition for extending the ornamental period of colorful leaf osmanthus, characterized in that, The composition consists of Composition I and Composition II. The raw materials of Composition I are ethephon solution, brassinolide solution and sucrose solution; the mass concentrations of the ethephon solution, brassinolide solution and sucrose solution are 100 - 300 mg / L, 0.2 - 0.6 mg / L and 5 - 15 g / L respectively; the volume ratio is ethephon solution:brassinolide solution:sucrose solution = 1:0.8 - 1.2:0.8 - 1.2; The raw materials of Composition II are abscisic acid solution, methyl jasmonate solution and sucrose solution; the mass concentrations of the abscisic acid solution, methyl jasmonate solution and sucrose solution are 50 - 150 mg / L, 10 - 50 mg / L and 5 - 15 g / L respectively; the volume ratio is abscisic acid solution:methyl jasmonate solution:sucrose solution = 1:0.8 - 1.2:0.8 - 1.2; The solvents of the ethephon solution, brassinolide solution, abscisic acid solution, methyl jasmonate solution and sucrose solution are a mixed solution of Tween - 20 with a mass concentration of 0.01 - 0.03% and ethanol with a mass concentration of 0.5 - 1.5%.

2. The composition according to claim 1, characterized in that, The preparation method of Composition I includes: dissolving ethephon, brassinolide and sucrose respectively with the solvent to prepare an ethephon solution, a brassinolide solution and a sucrose solution, and then mixing them to obtain the product.

3. The composition according to claim 1, wherein The preparation method of Composition II includes: dissolving abscisic acid, methyl jasmonate and sucrose respectively with the solvent to prepare an abscisic acid solution, a methyl jasmonate solution and a sucrose solution, and then mixing them to obtain the product.

4. Use of the composition according to any one of claims 1-3 in prolonging the ornamental period of Osmanthus fragrans 'Variegata', characterized in that, The colored - leaf osmanthus includes at least one of the red - leaf - type colored - leaf osmanthus varieties and the yellow - leaf - type colored - leaf osmanthus varieties; the yellow - leaf - type colored - leaf osmanthus is sprayed with Composition I, and the red - leaf - type colored - leaf osmanthus is sprayed with Composition I and Composition II.

5. The application according to claim 4 for prolonging the ornamental period of colorful osmanthus, characterized in that, The yellow - leaf - type colored - leaf osmanthus is sprayed with Composition I 4 - 6 days after the new leaves germinate, and Composition I is sprayed again 10 - 15 days later; the red - leaf - type colored - leaf osmanthus is sprayed with Composition II 4 - 6 days after the new leaves germinate, and Composition II is sprayed again 10 - 15 days later. 3 - 5 days after the leaves turn yellow, Composition I is sprayed.

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