A skin-delaying composition and a skin-care product

Abstract

The present invention belongs to the technical field of cosmetics, and discloses a skin delaying composition and a skin care product, wherein the skin delaying composition comprises white truffle extract, black truffle extract and lindera serrata root extract. The skin care product comprises the above-mentioned skin delaying composition, and the content of the skin delaying composition is ≥2%. The skin delaying composition provided by the present invention regulates the expression of circadian rhythm factors through lindera serrata root extract, maintains skin homeostasis, avoids abnormal cycle synchronization of skin silver clock genes leading to skin cell aging and skin wrinkles, and black truffle removes free radicals to a certain extent, and delays skin aging in two directions synchronously, and under the compounding of white truffle, the skin delaying effect is further improved, and a synergistic skin anti-aging effect is produced.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin anti-aging composition and a skin care product. Background Art

[0002] With the economic development of society, the pace of life is getting faster and faster, and nightlife is becoming more and more abundant. The daily routine of modern people has changed accordingly. People often stay up late for work or nightlife, which accelerates the aging of the skin. The skin is composed of collagen and elastin. The state of the skin depends on the loss of protein and the ability of cells in the skin to synthesize protein. The degradation and denaturation of elastin after degradation will cause the skin to lose elasticity, loosen, and wrinkle. Skin aging is related to the weakening of antioxidant function and the increase of free radicals in the body. In addition, the skin ages naturally with the increase of age. It is manifested as whitening of the skin, the appearance of fine wrinkles, decreased elasticity, and sagging skin.

[0003] The products on the market mainly remove free radicals, increase the rate of cell proliferation, slow down the degradation of the extracellular matrix, and provide the skin with sufficient nutrients to achieve deep nutrition. At the same time, they also slow down the loss of water in the skin to protect the skin.

[0004] Just like D1: Chinese patent 202211070515.X discloses a cosmetic composition containing nanoliposomes. It uses a nanolipid carrier compounded with hexapeptide and retinol, which can locally block nerves from transmitting muscle contraction information, relax facial muscles, increase the activity of elastin, improve relaxation, smooth dynamic lines, static lines and fine lines, improve the dispersibility and water solubility of fat-soluble vitamins, change the stability and absorption rate of active substances hexapeptide and retinol, and improve the utilization rate of retinol and hexapeptide through synergistic matching with other excipients, accelerate the renewal rate of deep skin cells, promote the skin to produce more epidermal proteins, remove free radicals, make the skin smoother and elastic, and play an anti-wrinkle and anti-aging role.

[0005] D2: Chinese patent 202110672445.4 discloses a free radical scavenging and anti-aging cosmetic composition, which can scavenge free radicals, reduce oxidative damage, resist cell oxidative damage and cell aging through the synergistic effect of imidazo[1,2-a]pyridine compounds and pyrazine-oxazole biaryl compounds, and effectively exert an antioxidant effect. When applied in cosmetics, it can give the cosmetics a significant anti-aging effect, making the skin more delicate, fair and moisturized.

[0006] D3: Chinese patent 202110248452.1 discloses an anti-aging composition with free radical scavenging function and its preparation method, which is mainly prepared from fish collagen peptide powder, grapefruit powder, cranberry powder, L-arabinose, acerola fruit powder, Haematococcus pluvialis, bird's nest peptide, mogroside and other raw materials. The fish skin is processed by a specific process to obtain a unique fish collagen peptide powder, and grapefruit, cranberry and acerola fruit are processed by a specific process, and compounded with other ingredients to prepare a composition with high free radical scavenging activity and high anti-aging performance, which is easy to eat and has a pleasant taste.

[0007] The above patent achieves the effect of delaying skin aging by scavenging free radicals. However, to delay skin aging, there is not only one direction to choose by scavenging free radicals. More directions should be developed to enhance the skin's anti-aging ability.

[0008] For the skin, circadian factors promote the protective functions of the environment during the day and enter a repair and regeneration mode at night. The biological clock is governed by the expression of specific genes called oscillators or pacemakers, including essential genes called clock genes, the circadian factors. These genes control key cellular mechanisms such as proliferation, metabolism, stress response and DNA repair. By enabling optimal functioning of protective and regenerative mechanisms, the biological clock ensures skin homeostasis.

[0009] However, stress or aging factors lead to the disruption of skin biohormones, resulting in desynchronization of metabolic processes and premature aging of tissues. Once they are desynchronized, cells can no longer adapt their behavior to changes in the environment. Their physiological functions slow down, their autoprotection and autorepair capabilities are poor, and they age faster.

[0010] Therefore, there is a need for an anti-aging composition that can regulate the expression of circadian rhythm factors and effectively delay skin aging.

[0011] The root extract of Linderae serrata can increase the night gene BMAL1 and the day gene PER, strengthen the circadian rhythm of skin genes, enhance the amplitude of genes, effectively regulate the expression of circadian rhythm factors, and prompt clock genes to restore normal cycle synchronization.

[0012] In this regard, the applicant has conducted further research on the extract of the root of Linderae umbellatae to improve the circadian rhythm factor regulating effect of the extract of the root of Linderae umbellatae. Summary of the invention

[0013] One of the purposes of the present invention is to provide a skin anti-aging composition, which delays skin aging from a different direction compared to the prior art, studies and compounds the extract of the root of Linderae umbellatae, and starts from the direction of regulating circadian rhythm factors, so that the skin's anti-aging ability is improved.

[0014] Another object of the present invention is to provide a skin care product, which comprises the above-mentioned skin delaying composition and has good skin delaying effect.

[0015] To achieve the above object, the present invention provides a skin anti-aging composition, comprising white truffle extract, black truffle extract and Linderae serrata root extract.

[0016] Preferably, based on weight percentage, it comprises 20-50% white truffle extract, 20-50% black truffle extract, and 10-30% Linderae umbellatae root extract.

[0017] Meanwhile, the present invention also provides a skin care product, comprising the above-mentioned skin delaying aging composition, wherein the content of the skin delaying aging composition is ≥2%.

[0018] Furthermore, the content of the skin-delaying composition in the skin care product is 2-20%.

[0019] Beneficial Effects

[0020] Compared with the prior art, the present invention has at least the following advantages:

[0021] (1) The skin anti-aging composition provided by the present invention regulates the expression of circadian rhythm factors through the extract of the root of Linderae umbellatae, maintains the homeostasis of the skin, and avoids the abnormal synchronization of the cycle of the silver clock gene of the skin, which leads to skin cell aging and skin wrinkles. At the same time, the black truffle extract removes free radicals to a certain extent, and the skin aging is delayed in two directions synchronously. In addition, under the compounding of the white truffle extract, the skin anti-aging effect is further improved, resulting in a synergistic skin anti-aging effect.

[0022] (2) The skin anti-aging composition provided by the present invention can significantly slow down cell aging and has an anti-aging effect on the skin through compound optimization. The white truffle extract, black truffle extract and Linderae umbellatae root extract are compounded within a certain range. The synergistic effect produced by the composition has a more obvious improvement effect than other anti-aging plant extracts.

[0023] (3) The skin anti-aging composition provided by the present invention has a significant anti-wrinkle effect on the skin, and the synergistic anti-wrinkle effect produced by the composition is significantly different from that of any combination of white truffle extract, black truffle extract, and Linderae umbellatae root extract.

[0024] (4) The present invention provides a skin care product comprising the above-mentioned skin delaying aging composition. When the content of the skin delaying aging composition is lower than 2%, the skin delaying aging effect of the skin care product is not obvious, and it needs to be higher than 2%. When the content is higher than 20%, the skin delaying aging effect of the skin care product is not significantly improved compared with the skin care product with a content of about 20%. Therefore, the preferred content range is 2-20%. DETAILED DESCRIPTION

[0025] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0026] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0027] In the following examples and comparative examples, the white truffle extract, black truffle extract, hydrolyzed yeast protein, gentian root extract, and ginseng extract were all purchased from Guangzhou Jianeng Biotechnology Co., Ltd., and the Linderae umbellatae root extract was purchased from Silab, France.

[0028] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0029] Unless otherwise specified, % and part in the present invention refer to percent by weight and part by weight.

[0030] Example 1

[0031] A skin anti-aging composition is obtained by uniformly mixing 35 parts of white truffle extracts, 35 parts of black truffle extracts and 30 parts of lindera agglomerata root extracts.

[0032] Example 2

[0033] A skin anti-aging composition is obtained by uniformly mixing 40 parts of white truffle extracts, 40 parts of black truffle extracts and 20 parts of lindera agglomerata root extracts.

[0034] Example 3

[0035] A skin anti-aging composition is obtained by uniformly mixing 45 parts of white truffle extracts, 45 parts of black truffle extracts and 10 parts of lindera agglomerata root extracts.

[0036] Example 4

[0037] A skin anti-aging composition is obtained by uniformly mixing 20 parts of white truffle extract, 50 parts of black truffle extract and 30 parts of lindera agglomerata root extract.

[0038] Example 5

[0039] A skin anti-aging composition is obtained by uniformly mixing 50 parts of white truffle extract, 20 parts of black truffle extract and 30 parts of lindera agglomerata root extract.

[0040] Comparative Example 1

[0041] A skin anti-aging composition is obtained by uniformly mixing 35 parts of white truffle extract, 35 parts of black truffle extract and 30 parts of hydrolyzed yeast protein.

[0042] Comparative Example 2

[0043] A skin anti-aging composition is obtained by uniformly mixing 35 parts of white truffle extract, 35 parts of ginseng extract and 30 parts of lindera serrata root extract.

[0044] Comparative Example 3

[0045] A skin anti-aging composition is obtained by uniformly mixing 35 parts of gentian extract, 35 parts of black truffle extract and 30 parts of lindera serrata root extract.

[0046] Comparative Example 4

[0047] A skin anti-aging composition is obtained by uniformly mixing 50 parts of white truffle extracts and 50 parts of black truffle extracts.

[0048] Comparative Example 5

[0049] A skin anti-aging composition is obtained by uniformly mixing 50 parts of white truffle extracts and 50 parts of lindera serrata root extracts.

[0050] Comparative Example 6

[0051] A skin anti-aging composition is obtained by uniformly mixing 50 parts of black truffle extracts and 50 parts of lindera serrata root extracts.

[0052] 1. SA-β-gal staining experiment

[0053] Experimental background

[0054] SA-β-gal staining experiment is a classic and widely recognized method for testing cell aging.

[0055] Most normal cells are thought to have only a limited ability to divide, and when they are unable to divide, they enter a state of senescence, in which the cells are still alive, but the expression profiles of their genes and proteins have changed significantly.

[0056] Senescent cells can no longer induce cell division under some conventional stimuli, and the cell cycle distribution of senescent cells is also quite special, which is different from some damage-induced cell dormancy and cell growth contact inhibition. Senescent cells usually become larger in size and express β-galactosidase, which has high enzyme activity at pH 6.0. Cell senescence is also considered a way for organisms to suppress tumors and a potential cause of aging.

[0057] Using X-Gal as a substrate, a dark blue product is generated under the catalysis of aging-specific β-galactosidase, so that cells or tissues expressing β-galactosidase that have turned blue can be easily observed under an optical microscope.

[0058] Experimental setup

[0059] The cells were divided into control group, sample group, aging model group, and aging model + sample group. The optimal concentration was selected for sample treatment.

[0060] Control group: Human skin cells were inoculated in a 6-well plate at a certain concentration, and after incubation for 24 hours, the solution in the wells was removed. 100 μL of DMDM ​​solution was added to each well and incubated in an incubator for 24 hours.

[0061] Sample group: Human skin cells were inoculated in a 6-well plate at a certain concentration, and after incubation for 24 hours, the solution in the wells was removed. 100 μL of sample solution was added to each well and incubated in an incubator for 24 hours.

[0062] Aging model group: Human skin cells were inoculated in a 6-well plate at a certain concentration, and after incubation for 24 hours, the solution in the wells was removed. 100 μL of hydrogen peroxide with a cell viability of about 70% was added to each well, incubated for 1 hour, and 100 μL of DMDM ​​was used as the control group, the solution in the wells was removed, and PBS was used to rinse twice, and 100 μL of DMDM ​​solution was added to each well, and incubated in an incubator for 24 hours.

[0063] Aging model group + sample group: Human skin cells were inoculated in a 6-well plate at a certain concentration, and after incubation for 24 hours, the solution in the wells was removed. 100 μL of hydrogen peroxide with a cell viability of about 70% was added to each well, incubated for 1 hour, and 100 μL of DMDM ​​was used as the control group, the solution in the wells was removed, and PBS was used to rinse twice, and 100 μL of sample solution was added to each well, and incubated in an incubator for 24 hours.

[0064] Experimental methods

[0065] (1) Treatment of test samples (cell viability test)

[0066] Sample group: The sample was diluted with pure water to a solution with a volume fraction of 4%, and then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0067] Negative control: basal culture medium.

[0068] (2) Treatment of test samples (human fibroblast β-galactosidase staining test)

[0069] Sample group: basal culture medium containing H2O2 and samples.

[0070] Negative control group: basic culture medium containing H2O2

[0071] (3) Test operation steps

[0072] Cell activity test: human fibroblasts were plated in 96-well plates. After 24 hours, the culture medium was discarded and basic culture medium containing different concentrations of test samples was added. After 24 hours, OD490nm was detected by MTT method and the effect of test samples on human fibroblast activity was analyzed by t-test.

[0073] Human fibroblast β-galactosidase staining test: human fibroblasts were plated on a 6-well plate and cultured for 24 hours, then β-galactosidase fixative was added and fixed at room temperature for 15 minutes, then β-galactosidase staining working solution was added to each well and incubated at 37°C overnight. Blue-stained cells were observed under an ordinary optical microscope.

[0074] (4) Data analysis

[0075] Image-pro plus (IPP) image analysis software was used for quantitative analysis of the staining images.

[0076] Experimental Results

[0077] Each raw material involved in Examples 1-5 and Comparative Examples 1-6 was tested according to the above experimental method and data analysis was performed. The test results are shown in Table 1 below;

[0078] Table 1 Test data before single raw material combination

[0079]

[0080]

[0081] According to the data in Table 1, the raw material data analysis was made, see Table 2 for details;

[0082] Table 2 Data analysis of single raw material

[0083]

[0084] The examples 1-5 and comparative examples 1-6 were tested and data analyzed according to the above experimental method. The results are shown in Table 3.

[0085] Table 3 Test data and analysis of the composition

[0086]

[0087]

[0088] Note: A significant P value of <0.050 indicates that there is a significant difference between the sample and the negative control; a significant P value of ≥0.050 indicates that there is no significant difference between the sample and the negative control;

[0089] Actual improvement rate / %: (negative control-sample) / negative control*100%;

[0090] Theoretical improvement rate / %: The improvement rate is calculated based on the actual improvement rate results of the individual raw materials in Table 2 and the weighted average according to the preparation ratio of the corresponding combination, for example:

[0091] The estimated improvement rate of Example 1 = 35%*19.66+35%*48.426+30%*12.117=27.465%.

[0092] According to the data in Table 3:

[0093] According to the data comparison of Example 1 and Comparative Examples 1-3, it can be seen that the present invention uses black truffle extract, white truffle extract and Lindera umbellatae root extract to produce significant synergistic effects, greatly improving the cell delaying effect. Compared with other extract combinations with similar effects, the effect achieved by the technical scheme of the present invention is more significant; among them, when Comparative Example 2 uses ginseng extract similar to black truffle to compound with white truffle extract and Lindera umbellatae root extract, not only can the expected cell delaying effect not be achieved, but the cell delaying effect is also greatly reduced. Therefore, the present invention uses black truffle extract, white truffle extract and Lindera umbellatae root extract to achieve a synergistic cell delaying effect that cannot be obtained by replacing other similar extracts; Comparative Examples 1 and Comparative Example 3 use hydrolyzed yeast protein and gentian extract to replace Lindera umbellatae root extract and white truffle extract respectively. Although the compounding of the three extracts has increased compared to the expected delaying effect, the increase in effect is not obvious. Therefore, it can be known that the synergistic effect of the three extracts in the technical scheme of the present invention is significant, and the compounding of other extracts does not have a synergistic cell delaying effect.

[0094] According to the data comparison of Example 1 and Comparative Examples 4-6, it can be seen that in the technical scheme of the present invention, the synergistic effect produced by black truffle extract, white truffle extract and Linderae umbellatae extract can only be achieved when the three are present at the same time, and the lack of any one of the extracts will greatly weaken the cell aging delay effect; especially in Comparative Example 4, in the absence of Linderae umbellatae root extract, the combination of white truffle extract and black truffle extract not only did not produce a synergistic effect, but its cell aging delay effect was also greatly reduced compared with the estimated cell aging delay effect, while although Comparative Examples 5 and Comparative Examples 6 did not produce significant cell aging delay effects after respectively lacking black truffle extract and white truffle extract, the cell aging delay effect was improved compared with the estimated cell aging delay effect, so the skin aging delay direction of the present invention that regulates the expression of circadian rhythm factors and scavenges free radicals simultaneously is feasible and effective; and when the technical scheme of the present invention is adopted, the combination of black truffle extract, white truffle extract and Linderae umbellatae extract produces a significant synergistic effect, which greatly improves the skin aging delay effect.

[0095] 1.2 Human fibroblast β-galactosidase staining test:

[0096] The HMC-WI-088 human fibroblast β-galactosidase staining test was performed according to the above experimental method, and the results are as follows:

[0097] 1. In the negative control group, there were more blue areas in the β-galactosidase staining of human fibroblasts;

[0098] 2. When the concentration of Example 1-3 was 0.5%, the cell activity was high, and the blue area in the human fibroblast β-galactosidase staining was small, accounting for 0.65-0.80%. Compared with the negative control, the blue area was reduced by 75-80%, which was significantly different from the negative control group (P < 0.05). The results showed that the sample had the effect of delaying aging and anti-wrinkle.

[0099] 3. When the concentration of Comparative Examples 1 and 3 was 0.5%, the cell activity was high, and the blue area in the human fibroblast β-galactosidase staining was less, accounting for 1.35-1.45%, and the blue area was reduced by 56-58.5% compared with the negative control, which was significantly different from the negative control group (P < 0.05). The results showed that the samples had the effect of delaying aging and anti-wrinkle, but were significantly weaker than Examples 1-3;

[0100] 4. When the concentration of comparative example 6 was 0.5%, the cell activity was high, and the blue area in the human fibroblast β-galactosidase staining was less, accounting for 1.851%, and the blue area was reduced by 43.601% compared with the negative control, which was significantly different from the negative control group (P < 0.05). The results showed that the sample had the effect of delaying aging and anti-wrinkle, but was significantly weaker than that of Examples 1-3;

[0101] 5. When the concentration of Comparative Examples 2, 4 and 5 was 0.5%, the cell activity was higher, and the blue area in the human fibroblast β-galactosidase staining was smaller, accounting for 2.7-2.95%. The blue area was reduced by 11-17.5% compared with the negative control, and there was no significant difference compared with the negative control group (P≥0.05).

[0102] 2. Skin wrinkle test

[0103] 2.1 Purpose of the experiment

[0104] The subjects were randomly assigned to use different test substances on both sides of their cheeks for 28 consecutive days, and their anti-aging effects were comprehensively evaluated by testing facial skin elasticity R2, firmness F4, and the number, depth, length, and area of ​​wrinkles.

[0105] 2.2 Test basis

[0106] 1) "Technical Specifications for Safety of Cosmetics" (2015 Edition)

[0107] 2) T / GDCDC 019-2021

[0108] 3)T / TDCA003-2021

[0109] 2.3 Test substances

[0110]

[0111]

[0112] 2.4 Subjects

[0113] 1) Healthy people aged 30 years and above

[0114] 2) There are obvious fine lines or wrinkles at the corners of the eyes and the elasticity value R2 is less than 0.8

[0115] 3) The test site has not participated in other clinical trials at present or in the past three months

[0116] 4) Those without severe systemic diseases, immune deficiency or autoimmune diseases, and the tested area has not received skin treatment, cosmetic treatment or other tests that may affect the results

[0117] 5) Those who are not highly sensitive

[0118] 6) Those who have not used hormone drugs or immunosuppressants in the past month

[0119] 7) Volunteer to participate in the trial and willing to sign the informed consent form

[0120] 8) Does not violate exclusion criteria and can comply with restrictions

[0121] Exclusion criteria

[0122] 1) People with irregular menstruation

[0123] 2) Have ever had severe skin disease symptoms due to the use of cosmetics

[0124] 3) Use of topical steroids, tacrolimus hydrate ointment, or nonsteroidal anti-inflammatory topical agents on the test site within 4 weeks before the start of the trial

[0125] 4) Suffering from comorbidities that may affect the evaluation of this trial

[0126] 5) Others that are deemed inappropriate by the responsible physician and the physician who shared the responsibility for the trial

[0127] 2.5 Instruments and Equipment

[0128] Instrument Name Model Specifications Skin elasticity tester Cutometer MPA580 Skin fast optical imaging system PRIMOS-CR

[0129] 2.6 Comparative statistical analysis of results

[0130] SPSS software was used to perform descriptive statistics on each test value. The Shapiro-Wilk Test was used to test the significance of the normal distribution of the data change value. If P>0.05, it was normally distributed, and the paired sample t test was performed, with a significant difference level of α=0.05. If P<0.05, it was non-normally distributed, and the Wilcoxon signed rank test was performed.

[0131] Test description:

[0132] Test Description

[0133] 1) Skin elasticity R2 value

[0134] Parameter description: reflects the ability of the skin to return to its original shape after being stretched or compressed; the larger the measured value, the better the skin elasticity.

[0135] Result judgment: Before and after trial use of the product, the R2 value of skin elasticity increased significantly (P < 0.05), indicating that the test substance has the effect of improving skin elasticity.

[0136] 2) Skin firmness F4 value: Using an elasticity tester, the overall amplitude logarithmic curve area of ​​the skin during ten consecutive negative pressure cycles.

[0137] Parameter description: reflects the firmness of the skin; the smaller the measured value, the firmer the skin.

[0138] 3) Anti-wrinkle: depth, number, area and length of skin wrinkles.

[0139] (1) Effect of composition content on skin care products

[0140] The formula of the essence containing the anti-aging composition was prepared according to Table 4, and the amount of each substance was expressed by mass fraction. Then, the skin wrinkle test was carried out according to the above test method. The results are shown in Table 5.

[0141] Among them, there are a total of 8 groups of test substances, which are divided into 8 groups on average, and a total of 32×8 volunteers are recruited to ensure that the final recovery data meets more than 30 people in each group.

[0142] Table 4: Sample formula of serum with different contents of the anti-aging composition of Example 1

[0143] Composition Blank control Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Deionized water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Xanthan gum 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Example 1 Composition 0 1.0 2.0 5.0 10.0 15.0 20.0 22.0 preservative Moderate Moderate Moderate Moderate Moderate Moderate Moderate Moderate glycerin 2 2 2 2 2 2 2 2 total 100 100 100 100 100 100 100 100

[0144] Table 5 Skin wrinkle test results of the composition of Example 1 at different concentrations

[0145]

[0146]

[0147] According to Table 5, we can see that:

[0148] There were no significant differences in the skin wrinkle test data between the blank control and sample 1 after Day 0 and Day 28; there were significant differences in the skin wrinkle test data between samples 2-7 after Day 0 and Day 28; but the difference in the rate of change of sample 7 relative to sample 6 was not large, and the skin anti-wrinkle and skin anti-aging effects of sample 6 after Day 28 were better than those of sample 7, indicating that the content of the skin anti-aging composition of the present invention in skin care products is best at 20%, and when the content of the skin anti-aging composition of the present invention is higher than 20%, although there is a significant anti-wrinkle effect, the increase in concentration is of little significance; the above skin wrinkle test data results of the blank control and samples 1-7 indicate that the blank control and 1.0% composition have little effect on skin anti-wrinkle and skin anti-aging, and 2.0-20.0% concentration is more preferred for the composition, and with the increase in concentration, the skin anti-wrinkle effect is more significant; but when the concentration of the skin anti-aging composition of the present invention is greater than 20%, the effect has not been significantly improved.

[0149] Therefore, in the skin care product of the present invention, the content of the skin delaying composition of the present invention is preferably 2-20%.

[0150] (2) Effects of different compositions on the anti-wrinkle effect of skin care products

[0151] Examples 1-5 and Comparative Examples 1-6 were respectively prepared into essences containing 2% of the anti-aging composition according to the formula of Sample 2 in Table 4, and then the skin anti-wrinkle results of Examples 1-5 and Comparative Examples 1-6 were obtained according to the above skin wrinkle test, as shown in Tables 6 to 10 for details.

[0152] Table 6 Analysis of skin elasticity R2 results of essence containing 2% of the anti-aging composition of Examples 1-5 and Comparative Examples 1-6

[0153] Group D0 D28 Rate of change P-value Significance Example 1 0.789 0.844 6.97％ ＜0.001 √ Example 2 0.785 0.842 7.26％ ＜0.001 √ Example 3 0.791 0.842 6.45％ ＜0.001 √ Example 4 0.791 0.827 4.55％ ＜0.05 √ Example 5 0.788 0.822 4.31％ ＜0.05 √ Comparative Example 1 0.792 0.788 -0.51％ ＞0.05 × Comparative Example 2 0.787 0.805 2.29％ ＜0.05 √ Comparative Example 3 0.786 0.789 0.38％ ＞0.05 × Comparative Example 4 0.789 0.812 2.92％ ＜0.05 √ Comparative Example 5 0.792 0.816 3.03％ ＜0.05 √ Comparative Example 6 0.787 0.791 0.51％ ＞0.05 ×

[0154] Table 7 Analysis of skin firmness F4 results of essence containing 2% of the anti-aging composition of Examples 1-5 and Comparative Examples 1-6

[0155]

[0156]

[0157] Table 8 Analysis of wrinkle depth results of essence containing 2% of anti-aging composition of Examples 1-5 and Comparative Examples 1-6

[0158] Group D0 D28 Rate of change P-value Significance Example 1 57 47 -17.54％ ＜0.001 √ Example 2 59 49 -16.95％ ＜0.001 √ Example 3 56 45 -19.64％ ＜0.001 √ Example 4 60 52 -13.33％ ＜0.05 √ Example 5 61 52 -14.75％ ＜0.05 √ Comparative Example 1 57 57 0.00％ ＞0.05 × Comparative Example 2 58 53 -8.62％ ＜0.05 √ Comparative Example 3 61 60 -1.64％ ＞0.05 × Comparative Example 4 57 51 -10.53％ ＜0.05 √ Comparative Example 5 59 54 -8.47％ ＜0.05 √ Comparative Example 6 61 62 1.64％ ＞0.05 ×

[0159] Table 9 Analysis of wrinkle counts of serum containing 2% of the anti-aging composition of Examples 1-5 and Comparative Examples 1-6

[0160]

[0161]

[0162] Table 10 Analysis of wrinkle area results of the essence containing 2% of the anti-aging composition of Examples 1-5 and Comparative Examples 1-6

[0163] Group D0 D28 Rate of change P-value Significance Example 1 122.47 105.89 -13.54％ ＜0.001 √ Example 2 123.01 101.73 -17.30％ ＜0.001 √ Example 3 121.33 103.81 -14.44％ ＜0.001 √ Example 4 120.11 107.31 -10.66％ ＜0.05 √ Example 5 118.87 106.31 -10.57％ ＜0.05 √ Comparative Example 1 119.89 118.02 -1.56％ ＞0.05 × Comparative Example 2 123.31 112.92 -8.43％ ＜0.05 √ Comparative Example 3 119.79 119.23 -0.47％ ＞0.05 × Comparative Example 4 122.02 113.08 -7.33％ ＜0.05 √ Comparative Example 5 118.97 110.89 -6.79％ ＜0.05 √ Comparative Example 6 118.96 117.31 -1.39％ ＞0.05 ×

[0164] According to the data in Tables 6 to 10:

[0165] According to the data of Examples 1 to 5, the skin anti-aging composition obtained by compounding white truffle extract, black truffle extract and Linderae serrata root extract of the present invention has significant effects in improving skin elasticity, improving skin firmness, reducing the depth of skin wrinkles, reducing the number of skin wrinkles and reducing the area of ​​skin wrinkles.

[0166] Comparative Example 1 uses hydrolyzed yeast protein to replace the extract of the root of Linderae umbellatae, but there is no obvious anti-wrinkle effect on the skin. Therefore, the role of the extract of the root of Linderae umbellatae in the composition in the technical solution of the present invention is irreplaceable.

[0167] Although the technical solution of using ginseng extract to replace black truffle extract in Comparative Example 2 has a more significant effect in improving skin firmness, the effects in other aspects are significantly different from those of the technical solution of the present invention.

[0168] In Example 3, I used gentian extract to replace white truffle extract, and it also did not produce obvious anti-wrinkle effect on the skin. Therefore, the role of white truffle extract in the composition in the technical solution of the present invention is irreplaceable.

[0169] The data of Comparative Examples 4-6 show that white truffle extract plays a more obvious role in skin anti-wrinkle. By comparing the data of Examples 1-5 of the present invention with Comparative Examples 4-6, it can be seen that the present invention uses black truffle extract and Linderae serrata root extract to compound with white truffle extract, which can effectively improve the skin anti-wrinkle effect of white truffle extract, and produce synergistic effects of improving skin elasticity, improving skin firmness, reducing skin wrinkle depth, reducing the number of skin wrinkles and reducing skin wrinkle area.

[0170] It is speculated that the mechanism of action of the skin anti-aging composition of the present invention is as follows: Linderae umbellata extract can regulate circadian rhythm factors, promote the periodic and regular expression of related genes, so that the skin can maintain a normal clock rhythm, and ultimately the protective ability is stronger during the day to reduce skin damage, and the repair ability is stronger at night to enhance skin recovery efficiency; black truffle extract can effectively avoid the occurrence of oxidative stress damage in the overall microenvironment of the skin, and can better reduce the occurrence of skin damage during the day when combined with Linderae umbellata extract; white truffle extract effectively reduces the occurrence of inflammatory damage by inhibiting cyclooxygenase, and can better promote skin recovery at night when combined with Linderae umbellata extract; finally, the combination of the three extracts can repair daytime damage overnight and significantly alleviate and reduce signs of skin aging.

[0171] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A skin anti-aging composition, characterized in that: According to weight percentage, it is composed of 20-50% white truffle extract, 20-50% black truffle extract and 10-30% Linderae umbellatae root extract.

2. A skin care product, characterized in that: The composition comprises the skin delaying aging composition as claimed in claim 1, wherein the content of the skin delaying aging composition is ≥ 2%.

3. The skin care product according to claim 2, characterized in that: The content of the skin delaying aging composition is 2-20%.

Images