Bacteroides xylanisolvens capable of preventing gosling gout and its medical use

Abstract

A strain of Bacteroides xylanisolvens capable of preventing gout in goslings and its medical use are provided, and the application of the strain in livestock and poultry breeding is also provided. The Bacteroides xylanisolvens was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on March 28, 2023, and named Bacteroides ( Bacteroides sp ), with the deposit number of CGMCC No. 26995. This strain can improve the growth performance of goslings, reduce the serum uric acid value of goslings, has no harmful effects on the liver and kidney functions of goslings, can enhance the expression of uric acid excretion-related enzymes and reduce the expression of uric acid production-related enzymes, can prevent gout in goslings, has the potential to improve the economic benefits of farmers, and has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to a Bacteroides xylanisolvens strain capable of preventing gosling gout and its medical use, and can be made into a drug for preventing and treating gosling gout, belonging to the field of microbial technology. Background Art

[0002] Gosling gout is a chronic inflammatory disease with a relatively high incidence and lethality rate caused by urate deposition. Since goslings lack uricase and arginase, purines and proteins ingested into their bodies will be metabolized into uric acid. Therefore, feeding goslings with high-protein diets can cause goslings to produce too much uric acid and thus trigger gout. At the same time, the research on the association between intestinal flora and various diseases has become a current research hotspot, and relevant research has confirmed that there is a certain correlation between gout and intestinal flora, that is, there are differences in the composition of multiple strains of intestinal flora between healthy goslings and gosling gout, including Bacteroides xylanisolvens.

[0003] Bacteroides xylanisolvens is a Gram-negative strict anaerobe and can only grow well in a completely anaerobic environment. Bacteroides xylanisolvens can metabolize polysaccharides such as xylan, and can also metabolize small molecule sugars such as hemicellulose and xylose. The compound utilization system of Bacteroides xylanisolvens is significantly better than that of other Bacteroides species and can survive with high quality in different carbon source environments. At the same time, the immunomodulatory effect and strong environmental adaptability of Bacteroides xylanisolvens are one of the necessary characteristics of the new generation of prebiotics and probiotics sought by microbiologists.

[0004] Currently, there is a view that Bacteroides may become the "new generation of probiotics". For example, the Implementing Decision No. 2015 / 1291 of the European Commission (EU) approved Bacteroides xylanisolvens DSM23964 to be put on the market as a starter culture for inactivated fermented dairy products; research shows that Bacteroides xylanisolvens FCYS01 can prevent and treat alcohol-induced alcoholic liver disease; research shows that Bacteroides xylanisolvens NSP003 can improve the disease activity index and splenomegaly of mice with ulcerative colitis, maintain the integrity of the intestinal barrier, and reduce the level of oxidative stress. The above examples illustrate that Bacteroides xylanisolvens has the potential for probiotic applications.

[0005] In recent years, due to the continuous expansion of the breeding quantity and production scale of the goose farming industry, the high incidence and lethality rate of gosling gout also trouble farmers. However, the current application of Bacteroides xylanisolvens in goslings is still in a blank state. Therefore, it is very necessary to screen a probiotic strain capable of preventing gosling gout. Summary of the Invention

[0006] To solve the problem of gosling gout, the present invention provides a Bacteroides xylanisolvens BX2022 that can prevent gosling gout, and it has been proven that in animal experiments, it can improve the growth performance of goslings, reduce the serum uric acid value of goslings, has no harmful effects on the liver and kidney functions of goslings, can enhance the expression of enzymes related to uric acid excretion and reduce the expression of enzymes related to uric acid production, has the potential to improve the economic benefits of farmers, and can be made into a drug for preventing and treating gosling gout.

[0007] The present invention screened a Bacteroides xylanisolvens BX2022 that can prevent gosling gout, which was deposited in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on March 28, 2023. The deposit address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 26995, and the taxonomic name is Bacteroides. (Bacteroides sp) .

[0008] After sequencing the isolated strain, the obtained sequence was subjected to nucleic acid sequence alignment in NCBI. The results showed that the strain belongs to Bacteroides xylanisolvens, so it was named Bacteroides xylanisolvens ( Bacteroides xylanisolvens ) BX2022.

[0009] The Bacteroides xylanisolvens BX2022 has the following properties:

[0010] Improve the growth performance of goslings;

[0011] Can reduce the serum uric acid value of goslings;

[0012] Has no harmful effects on the liver and kidney functions of goslings;

[0013] Can enhance the expression of enzymes related to uric acid excretion and reduce the expression of enzymes related to uric acid production.

[0014] The cell characteristics of the Bacteroides xylanisolvens BX2022: Obvious colonies are formed on the medium, with a diameter between 0.3 - 2 mm. The front morphology is round, convex in the middle, with neat edges, slightly white, opaque, and the surface is moist and smooth.

[0015] The growth characteristics of the Bacteroides xylanisolvens BX2022: Gram-negative rod-shaped bacteria, strict anaerobes, sensitive to oxygen, with the best growth at a temperature of 35 - 38 °C, the optimal growth pH value is 6.6 - 7.0, grows well in a medium containing glucose, and can enter the late logarithmic or early stationary phase in 12 h.

[0016] The positive effects of the present invention are as follows: It provides a strain of Bacteroides xylanisolvens BX2022, which is a new strain. It can reduce the serum uric acid value of goslings, has no harmful effects on the liver and kidney functions of goslings, can enhance the expression of uric acid excretion-related enzymes and reduce the expression of uric acid production-related enzymes, has the potential to improve the economic benefits of farmers, and can be made into a drug for preventing and treating gosling gout. Brief Description of the Drawings

[0017] Figure 1 It is the colony morphology diagram of Bacteroides xylanisolvens BX2022;

[0018] Figure 2 It is the growth curve diagram of anaerobic culture of Bacteroides xylanisolvens BX2022 at 37°C;

[0019] Figure 3 It is the gene sequencing BLAST Tree of the bacterial solution of Bacteroides xylanisolvens BX2022;

[0020] Figure 4 It is the electrophoresis result diagram of the PCR product of Bacteroides xylanisolvens BX2022;

[0021] Figure 5 It is the comparison diagram of the total weight gain of goslings in the normal diet group and the normal diet + Bacteroides xylanisolvens BX2022 group;

[0022] Figure 6 It is the comparison diagram of the serum uric acid value (Serum UA) of 5 groups of goslings;

[0023] Figure 7 It is the comparison diagram of the liver and kidney functions of 5 groups of goslings: serum alanine aminotransferase (Serum ALT), serum aspartate aminotransferase (SerumAST), liver xanthine oxidase activity (Liver XOD activity); kidney function: serum urea nitrogen (Serum BUN), serum creatinine (Serum CREA);

[0024] Figure 8 It is the comparison diagram of the relative mRNA expression levels of glucose transporter 9 (GLUT9), ATP-binding cassette transporter G2 (ABCG2) in the kidneys of 5 groups of goslings; xanthine oxidase (XOD), adenosine deaminase (ADA), glucose transporter 9 (GLUT9) in the liver; Embodiment

[0025] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be described in detail below in combination with specific embodiments and drawings.

[0026] The culture media involved in the following embodiments are as follows:

[0027] Brain Heart Infusion Agar (BHI): Beef brain infusion powder 4.0 g / L, beef heart infusion powder 4.0 g / L, peptone 5.0 g / L, casein peptone 16.0 g / L, sodium chloride 5.0 g / L, glucose 2.0 g / L, disodium hydrogen phosphate 2.5 g / L, agar 13.5 g / L, pH value is 7.4 ± 0.2.

[0028] Brain Heart Infusion Broth (BHI): Tryptone 10.0 g / L, beef heart infusion powder 17.5 g / L, sodium chloride 5.0 g / L, glucose 2.0 g / L, disodium hydrogen phosphate (12H 2 O) 2.5 g / L, pH value is 7.4 ± 0.2.

[0029] The preparation method of the Bacteroides xylanisolvens bacterial suspension involved in the following examples is as follows:

[0030] Streak Bacteroides xylanisolvens on the Brain Heart Infusion Agar solid medium, culture at 37 °C in an anaerobic bag for 48 h to obtain single colonies; pick single colonies and inoculate them into the Brain Heart Infusion Broth liquid medium, culture at 37 °C for 12 h to obtain a bacterial solution; add glycerol with a total volume of 20 - 30% to the bacterial solution and store it at -80 °C. Example 1

[0031] Sample collection

[0032] Collect fecal samples from healthy three - flower goslings, store them in a preservation box with ice packs, and quickly place them in a -80 °C refrigerator for separation and screening after bringing them back to the laboratory.

[0033] 2. Isolation and culture of strains

[0034] 1) Gradient dilution of fecal samples: In a sterile anaerobic environment, take about 1 g of the fecal sample collected in step 1, add it to 9 mL of physiological saline to obtain the first gradient dilution, pipette 1 mL of the first gradient dilution into 9 mL of physiological saline to obtain the second gradient dilution, and so on, a total of 8 gradient dilutions are prepared; repeat the above dilution steps to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 dilutions;

[0035] 2) Spread plate culture: Pipette 100 μL of each of the above dilutions onto the Brain Heart Infusion Agar solid medium respectively, spread them, and culture at 37 °C under anaerobic conditions for 48 h to obtain dilution spread plates;

[0036] 3) Purification and culture: Pick different morphological colonies on brain heart infusion agar medium for streak isolation until pure single colonies with neat edges, slightly white, opaque, moist and smooth surfaces, and consistent morphology are obtained. The colony morphology can be seen Figure 1 ; Pick the pure colonies on brain heart infusion agar solid medium and inoculate them into 5 mL of brain heart infusion broth medium to obtain a purified culture solution. Place the purified culture solution under anaerobic conditions at 37 °C for 12 h until the late logarithmic phase or the early stationary phase. After the colony count reaches 1×10 10 CFU / mL, add glycerol with a total volume of 20%-30% and store it at -80 °C. The growth curve of Bacteroides xylanisolvens BX2022 can be seen Figure 2 .

[0037] 3. Identification of the strain

[0038] 1) Send the bacterial solution from step 2(3) to Jilin Kumei Biotechnology Co., Ltd. for gene sequencing; upload the spliced sequence of the sequencing to BLAST of NCBI (http: / / www.ncbi.nlm.nih.gov / BLAST) for species confirmation. Its 16S rDNA amplification sequence is shown in SEQ ID NO.1. The comparison result shows that it is Bacteroides xylanisolvens ( Bacteroides xylanisolvens ), and the BLAST Tree can be seen Figure 3 ;

[0039] 2) Extract the DNA of the bacterial solution from step 2(3) as the template for ordinary PCR amplification;

[0040] 3) The reaction program for the ordinary PCR amplification is as follows: Pre-denature at 94 °C for 5 min, and perform the following cycles: Denature at 94 °C for 30 s; Anneal at 60 °C for 30 s; Extend at 72 °C for 30 s; Run 35 cycles respectively, and finally extend at 72 °C for 5 min;

[0041] 4) Among them, the reaction system for the ordinary PCR amplification is 25 μL, and the reaction system is as follows: ddH 2 0, 8.5 μL; 1 μL each of the forward primer and the reverse primer; 2 μL of the bacterial solution DNA template; 12.5 μL of Taq DNA polymerase;

[0042] 5) Among them, the designed forward primer for Bacteroides xylanisolvens is: 5’-CGCTTTCAGCTCCTGTGAGA-3’, and the reverse primer is: 5’-ATGCCCACGAGTGATTGACC-3’;

[0043] 6) After the PCR products were confirmed by nucleic acid electrophoresis analysis, they were sent to Jilin Kumei Biotechnology Co., Ltd. for gene sequencing; the spliced sequences obtained by sequencing were uploaded to BLAST of NCBI (http: / / www.ncbi.nlm.nih.gov / BLAST) for species confirmation. The 16S rDNA amplification sequence was as shown in SEQ ID NO.2. The comparison results showed that it was confirmed to be Bacteroides xylanisolvens ( Bacteroides xylanisolvens ), and the results of nucleic acid electrophoresis can be seen Figure 4 .

[0044] 4. Preservation of the Strain

[0045] The Bacteroides xylanisolvens BX2022 was named Bacteroides ( Bacteroides sp. ) and the preservation unit was the General Microbiology Center of the China Committee for Culture Collection of Microorganisms. The preservation address was No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The preservation date was March 28, 2023, and the preservation number was CGMCC No. 26995.

[0046] Experimental Example 1

[0047] The specific steps are as follows:

[0048] 1. Preparation of the gastric infusion of Bacteroides xylanisolvens BX2022

[0049] The Bacteroides xylanisolvens BX2022 obtained in step 2 of Example 1 was streaked on brain heart infusion agar medium and cultured anaerobically at 37 °C for 48 h to obtain single colonies; the single colonies on the brain heart infusion agar medium were picked and inoculated into brain heart infusion broth liquid medium and cultured in a 37 °C incubator for 12 h to obtain a bacterial solution; the obtained bacterial solution was inoculated into a new brain heart infusion broth liquid medium at a ratio of 1:100 for amplified culture for 12 h. Glycerol with a total volume of 20 - 30% was added to the bacterial solution and aliquoted and stored at -80 °C. Before the experiment, the suspension of Bacteroides xylanisolvens BX2022 was centrifuged, the supernatant was poured off, and it was washed and resuspended with an equal volume of sterile normal saline to obtain the gastric infusion of Bacteroides xylanisolvens BX2022 (1×10 10 CFU / mL).

[0050] 2. Experimental Animals

[0051] One-day-old three-flower male goslings were raised on a wire bed with a warming lamp. The temperature was 32 ± 1 °C in the first 7 days of feeding and decreased by 2.5 ± 0.5 °C per week, and the final temperature was 26 °C. The animal room was kept clean and ventilated all the time.

[0052] 3. Experimental Grouping

[0053] Fifty one-day-old healthy goslings were randomly divided into 5 groups, with 10 in each group. The 5 groups were: normal diet group,

[0054] Normal diet + Bacteroides xylanisolvens BX2022 intervention group, high-protein diet group, high-protein diet + Bacteroides xylanisolvens BX2022 intervention group, high-protein diet + allopurinol intervention group (positive drug control group).

[0055] 4. Experimental content

[0056] 1) Normal diet group (NPD): Fed with normal diet;

[0057] 2) Normal diet + Bacteroides xylanisolvens BX2022 intervention group (NPD + BX): Fed with normal diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile physiological saline was added and mixed evenly for feeding to goslings;

[0058] 3) High-protein diet group (HPD): Fed with high-protein diet;

[0059] 4) High-protein diet + Bacteroides xylanisolvens BX2022 intervention group (HPD + BX): Fed with high-protein diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile physiological saline was added and mixed evenly for feeding to goslings;

[0060] 5) High-protein diet + allopurinol intervention group (HPD + ALLO): Fed with high-protein diet; Allopurinol was made into a suspension and fed to goslings at 10 mg / Kg;

[0061] 6) The weights of the 5 groups of goslings were measured and recorded every two days in the morning;

[0062] 7) The experimental period was 25 days.

[0063] 5. Experimental results

[0064] As Figure 5 shown, compared with the normal diet group, the total weight gain in the normal diet + Bacteroides xylanisolvens BX2022 intervention group increased, indicating that Bacteroides xylanisolvens BX2022 can improve the growth performance of goslings.

[0065] Experimental Example 2

[0066] The specific steps are as follows:

[0067] The preparation of the gastric lavage fluid of Bacteroides xylanisolvens BX2022 was the same as in Example 1.

[0068] The experimental animals were the same as in Example 2.

[0069] The experimental grouping was the same as in Example 2.

[0070] Experimental content

[0071] 1) Normal diet group (NPD): Fed with normal diet;

[0072] 2) Normal diet + Bacteroides xylanisolvens BX2022 intervention group (NPD+BX): Fed with normal diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile saline was added and mixed evenly, then fed to the goslings;

[0073] 3) High-protein diet group (HPD): Fed with high-protein diet;

[0074] 4) High-protein diet + Bacteroides xylanisolvens BX2022 intervention group (HPD+BX): Fed with high-protein diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile saline was added and mixed evenly, then fed to the goslings;

[0075] 5) High-protein diet + allopurinol intervention group (HPD+ALLO): Fed with high-protein diet; Allopurinol was made into a suspension and fed to the goslings at a dose of 10 mg / Kg;

[0076] 6) The experimental period was 25 days;

[0077] 7) On the 26th day, the goslings were sacrificed, blood was collected from the hearts of the goslings in 5 groups, and the serum uric acid value (SerumUA) was detected;

[0078] 5. Experimental results

[0079] As Figure 6 shown, compared with the high-protein diet group, the serum uric acid value of the high-protein diet + Bacteroides xylanisolvens BX2022 intervention group decreased significantly, indicating that Bacteroides xylanisolvens BX2022 can reduce the serum uric acid value of goslings.

[0080] Experimental Example 3

[0081] The specific steps are as follows:

[0082] 1) The preparation of the Bacteroides xylanisolvens BX2022 gastric lavage fluid was the same as in Example 1.

[0083] 2) The experimental animals were the same as in Example 2.

[0084] 3) The experimental grouping was the same as in Example 2.

[0085] 4) Experimental content

[0086] 1) Normal diet group (NPD): Fed with normal diet;

[0087] 2) Normal diet + Bacteroides xylanisolvens BX2022 intervention group (NPD+BX): Fed with normal diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile saline was added and mixed evenly, then fed to the goslings;

[0088] 3) High-protein diet group (HPD): Fed a high-protein diet;

[0089] 4) High-protein diet + Bacteroides xylanisolvens BX2022 intervention group (HPD+BX): Fed a high-protein diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile normal saline was added and mixed well, then fed to the goslings;

[0090] 5) High-protein diet + allopurinol intervention group (HPD+ALLO): Fed a high-protein diet; Allopurinol was made into a suspension and fed to the goslings at a dose of 10 mg / Kg;

[0091] 6) The experimental period was 25 days;

[0092] 7) On the 26th day, the goslings were sacrificed, blood of the 5 groups of goslings was collected by cardiac puncture, and the following liver-related indicators were detected: Serum alanine aminotransferase (Serum ALT), Serum aspartate aminotransferase (Serum AST); Kidney-related indicators: Serum urea nitrogen (Serum BUN) and Serum creatinine (Serum CREA);

[0093] 8) The liver tissues of the goslings were collected, and the activity of liver xanthine oxidase (Liver XOD activity) was detected.

[0094] 5. Experimental results

[0095] As Figure 7 shown, compared with the goslings in the normal diet group, the serum alanine aminotransferase, serum aspartate aminotransferase, serum urea nitrogen, serum creatinine and the activity of liver xanthine oxidase in the high-protein diet group were all increased. The above indicators in the high-protein diet + Bacteroides xylanisolvens BX2022 intervention group decreased compared with the high-protein diet group. There were no significant differences in the results of serum alanine aminotransferase, serum aspartate aminotransferase, serum urea nitrogen, serum creatinine and the activity of liver xanthine oxidase between the normal diet group and the normal diet + Bacteroides xylanisolvens BX2022 intervention group. It shows that the high-protein diet has damage to the liver and kidney functions, while Bacteroides xylanisolvens BX2022 can antagonize this damage and has no harmful effects on the liver and kidney functions of goslings.

[0096] Experimental Example 4

[0097] The specific steps are as follows:

[0098] 1. The preparation of the gastric lavage fluid of Bacteroides xylanisolvens BX2022 was the same as that in Example 1.

[0099] 2. The experimental animals were the same as those in Example 2.

[0100] 3. The experimental grouping was the same as that in Example 2.

[0101] 4. Experimental Contents

[0102] 1) Normal Diet Group (NPD): Fed with normal diet;

[0103] 2) Normal Diet + Bacteroides xylanisolvens BX2022 Intervention Group (NPD+BX): Fed with normal diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile physiological saline was added and mixed evenly, then fed to goslings;

[0104] 3) High-Protein Diet Group (HPD): Fed with high-protein diet;

[0105] 4) High-Protein Diet + Bacteroides xylanisolvens BX2022 Intervention Group (HPD+BX): Fed with high-protein diet; After centrifuging Bacteroides xylanisolvens BX2022, the supernatant was discarded, and an equal volume of sterile physiological saline was added and mixed evenly, then fed to goslings;

[0106] 5) High-Protein Diet + Allopurinol Intervention Group (HPD+ALLO): Fed with high-protein diet; Allopurinol was made into a suspension and fed to goslings at a dose of 10 mg / Kg;

[0107] 6) The experimental period was 25 days;

[0108] 7) On the 26th day, the goslings were sacrificed, and the liver and kidney tissues of the 5 groups of goslings were collected. The liver and kidney tissues were ground in liquid nitrogen to extract RNA, reverse transcribed into cDNA, and related indicators were detected by fluorescence quantitative PCR: xanthine oxidase (XOD), adenosine deaminase (ADA), glucose transporter 9 (GLUT9), and ATP-binding cassette transporter G2 (ABCG2).

[0109] 5. Experimental Results

[0110] As Figure 8 shown, compared with the high-protein diet group, the intervention of high-protein diet + Bacteroides xylanisolvens BX2022 could reduce the relative mRNA expression levels of xanthine oxidase (XOD) in the liver and kidney; reduce the relative mRNA expression level of adenosine deaminase (ADA) in the liver; increase the relative mRNA expression levels of glucose transporter 9 (GLUT9) in the liver and kidney; significantly increase the relative mRNA expression level of ATP-binding cassette transporter G2 (ABCG2) in the kidney. It shows that Bacteroides xylanisolvens BX2022 can enhance the expression of proteins related to uric acid excretion and reduce the expression of enzymes related to uric acid production.

[0111] Conclusion: The colony morphology of Bacteroides xylanisolvens BX2022 is shown in Figure 1 ; The anaerobic culture growth curve of Bacteroides xylanisolvens BX2022 is shown in Figure 2; The BLAST Tree of the gene sequencing of the Bacteroides xylanisolvens BX2022 bacterial solution is shown in Figure 3 ; The electrophoresis results of the PCR products of Bacteroides xylanisolvens BX2022 are shown in Figure 4 ; The comparison of the total weight gain between the goslings fed with normal diet and the goslings fed with normal diet + Bacteroides xylanisolvens BX2022 is shown in Figure 5 ; The comparison of the serum uric acid values of the five groups of goslings is shown in Figure 6 ; The comparison of the serum alanine aminotransferase (Serum ALT), serum aspartate aminotransferase (Serum AST), serum urea nitrogen (Serum BUN), serum creatinine (Serum CREA) and liver xanthine oxidase activity (Liver XOD activity) of the five groups of goslings is shown in Figure 7 ; The comparison of the relative mRNA expression levels of xanthine oxidase (XOD), adenosine deaminase (ADA), glucose transporter 9 (GLUT9), and ATP-binding cassette transporter G2 (ABCG2) in the livers and kidneys of the five groups of goslings is shown in Figure 8 . As can be seen from the figure, Bacteroides xylanisolvens BX2022 can improve the growth performance of goslings; can reduce the serum uric acid value of goslings; has no harmful effects on the liver and kidney functions of goslings; can increase the expression of enzymes related to uric acid excretion and reduce the expression of enzymes related to uric acid production; can prevent gout in goslings, has the potential value of improving the economic benefits of farmers, and has a wide application prospect.

Claims

1. Bacteroides xylanisolvens BX2022 was deposited at the China General Microbiological Culture Collection Center on March 28, 2023. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 26995, and the taxonomic name is Bacteroides (Bacteroides sp.) .

2. Use of Bacteroides xylanisolvens BX2022 according to claim 1 in the preparation of a preparation for improving the growth performance of goslings and preventing gout in goslings.

3. Use of Bacteroides xylanisolvens BX2022 according to claim 2 in the preparation of a preparation for improving the growth performance of goslings and preventing gosling gout, wherein the viable count of Bacteroides xylanisolvens BX2022 is not less than 1×10 10 CFU / mL or 1×10 10 CFU / g.

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