Page gel recovery device

Abstract

The application discloses a PAGE gel recovery device and relates to the technical field of biological experiment devices.The PAGE gel recovery device comprises a gel beating and liquid sucking mechanism and a fusion liquid supply mechanism.The gel beating and liquid sucking mechanism comprises a rack, a gel beating head, a deep hole plate, a sucking head, a gel beating mechanism and a liquid sucking mechanism.The working table surface of the rack is sequentially provided with a gel beating head placing seat, a deep hole plate placing seat and a sucking head placing seat along the X-axis direction.The deep hole plate placing seat is provided with a plurality of working zones along the X-axis direction.A working zone is provided with a heating device for heating the deep hole plate.The gel beating head is placed on the gel beating head placing seat.The deep hole plate is placed on the deep hole plate placing seat.The sucking head is placed on the sucking head placing seat.The gel beating mechanism is in communication with the fusion liquid supply mechanism through a hose.The gel beating mechanism is movably arranged on the rack.The gel beating mechanism grasps the gel beating head and moves to the position of the deep hole plate to beat the gel or add liquid.The liquid sucking mechanism is used for transferring the liquid of one deep hole plate to another deep hole plate.The PAGE gel recovery device improves the efficiency and is suitable for large batch detection.

Description

Technical Field

[0001] This invention relates to the field of biological experimental apparatus technology, and in particular to a PAGE gel recovery device. Background Technology

[0002] Polyacrylamide gel electrophoresis (PAGE) is a commonly used electrophoresis technique that uses polyacrylamide gel as a supporting medium to separate proteins and oligonucleotides.

[0003] Currently, the method for recovering DNA from polyacrylamide gels involves crushing the gel, adding a specific solution and mixing thoroughly, heating to fully dissolve the gel in the solution, and then using a pipette tip to extract DNA fragments from the solution. This process is currently manual, requires various tools and instruments, is costly and cumbersome, and is only suitable for small-batch testing. When dealing with a large number of gels requiring recovery, manual operation becomes inefficient. Summary of the Invention

[0004] The main objective of this invention is to provide a PAGE gel recovery device, which aims to provide an efficient PAGE gel recovery device suitable for high-volume testing.

[0005] To achieve the above objectives, the present invention proposes a PAGE gel recovery device, which includes an adjacently arranged mixing and liquid absorption mechanism and a fusion solution supply mechanism. The mixing and liquid absorption mechanism includes:

[0006] The frame has a worktable, and the worktable is provided with a tamping head placement seat, a deep hole plate placement seat and a suction head placement seat in sequence along the X-axis direction. The deep hole plate placement seat is provided with multiple working areas along the X-axis direction, and one of the working areas is provided with a heating device for heating the deep hole plate.

[0007] A tamping head, wherein the tamping head is placed in the tamping head placement seat;

[0008] A deep hole plate, wherein the deep hole plate is placed in a deep hole plate placement seat;

[0009] A suction head, which is placed in a suction head holder, has a filter element at its front end;

[0010] The mixing mechanism is used for mixing and adding liquid. The mixing mechanism is connected to the fusion liquid supply mechanism through a hose. The mixing mechanism is movably mounted on the frame. The mixing mechanism grabs the mixing head and moves it to the position of the deep hole plate to mix or add liquid.

[0011] A liquid suction mechanism is movably mounted on the frame and is used to transfer liquid from one deep-hole plate to another deep-hole plate.

[0012] Optionally, the mixing head placement seat has N first workstations along the Y-axis direction, each working area has N second workstations along the Y-axis direction, and the suction head placement seat has N third workstations along the Y-axis direction. The first workstations, second workstations, and third workstations are in a one-to-one correspondence.

[0013] Optionally, the tamping mechanism includes:

[0014] The first guide rail slides in the X-axis direction with the frame under the drive of the first power device;

[0015] The first sliding seat slides in the Y-axis direction with the first guide rail under the drive of the second power device.

[0016] The second sliding seat is slidably engaged with the first sliding seat in the Z-axis direction under the drive of the third power device;

[0017] The dispenser is connected to the fusion liquid supply mechanism via a hose and is connected to the second sliding seat via a connecting rod. The bottom of the dispenser is provided with a mounting groove, and the mixing head is located in the mounting groove and communicates with the dispenser. The mixing head has a liquid outlet hole for discharging liquid.

[0018] An electric gripper is used to hold the tamping head. The electric gripper is disposed on the second sliding seat and has two gripping parts located on opposite sides of the mounting groove.

[0019] Optionally, the dispenser is provided with multiple connectors connected to the fusion liquid supply mechanism, the bottom of the mounting groove is provided with multiple sets of drain ports to correspond to the multiple connectors, each set of drain ports includes multiple drain holes, the top of the mixing head is provided with multiple sets of inlets to correspond to the multiple drain ports, each set of inlets includes multiple inlet holes, the number of inlet holes and the position of the drain holes are the same, and the outlet hole is connected to the inlet hole.

[0020] Optionally, the mixing head includes a liquid inlet and a mixing part connected to each other, multiple sets of liquid inlets are provided in the liquid inlet, the mixing part is provided at the bottom of the liquid inlet, the mixing part has multiple parts and each corresponds to multiple sets of liquid inlets, and the liquid outlet is provided on the four sides of the mixing part.

[0021] Optionally, the mixing section includes a connecting section, a liquid outlet section, and a mixing section connected in sequence. The connecting section is connected to the bottom of the liquid inlet section, the liquid outlet holes are provided on the four sides of the liquid outlet section, and the sides and bottom of the mixing section are provided with mixing grooves.

[0022] Optionally, the length of the crushing section is defined as L1 and the width as D1, and the deep hole plate has multiple square holes, the length of the square holes is defined as L2 and the width as D2, where L1 < L2 and D1 < D2.

[0023] Optionally, the mixing mechanism further includes an anti-drip component, the anti-drip component comprising:

[0024] Connecting seat, the connecting seat being disposed on the first sliding seat;

[0025] A drain plate, which slides horizontally with the connecting seat under the drive of the fourth power device.

[0026] Optionally, the working area for mixing the adhesive is defined as the mixing working area, the heating device is located in the mixing working area, the heating device is provided with a heating groove for positioning the deep hole plate, and a clamping plate is detachably provided on the top of the heating device.

[0027] Optionally, the liquid suction mechanism includes:

[0028] The second guide rail slides in the X-axis direction with the frame under the drive of the fifth power device;

[0029] The third sliding seat is slidably engaged with the second guide rail in the Y-axis direction under the drive of the sixth power device;

[0030] The fourth sliding seat is slidably engaged with the third sliding seat in the Z-axis direction under the drive of the seventh power device;

[0031] A pipette, wherein the pipette is disposed on the fourth sliding seat.

[0032] The technical solution of this invention includes a mixing and liquid aspiration mechanism and a fusion liquid supply mechanism. The mixing and liquid aspiration mechanism includes a frame, a mixing head, a deep well plate, a suction head, a mixing mechanism, and a liquid aspiration mechanism. Multiple deep well plates are placed on a deep well plate holder. The mixing mechanism picks up the mixing head and moves it to the position of the deep well plate to mix the adhesive or add liquid. The fusion liquid in the deep well plate is heated by a heating device. The liquid aspiration mechanism is used to transfer the liquid from one deep well plate to another. Because it adopts automatic liquid supply, automatic mixing, automatic heating, and automatic liquid transfer, it effectively solves the technical problem of low efficiency caused by manual operation in the prior art, thereby achieving the technical effect of improving efficiency and being suitable for large-scale testing. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of an embodiment of the PAGE gel recovery device of the present invention;

[0034] Figure 2 This is a schematic diagram of the PAGE gel recovery device of the present invention from another perspective;

[0035] Figure 3 This is a schematic diagram of the structure of the worktable of the present invention;

[0036] Figure 4 This is a schematic diagram of the structure of the tamping head of the present invention;

[0037] Figure 5 This is a front view schematic diagram of the tamping head of the present invention;

[0038] Figure 6 This is a schematic diagram of the adhesive mixing mechanism of the present invention;

[0039] Figure 7 This is a schematic diagram of the adhesive mixing mechanism of the present invention from another perspective;

[0040] Figure 8 This is a schematic diagram of the adhesive mixing mechanism of the present invention, omitting the first guide rail;

[0041] Figure 9 for Figure 8 A structural diagram from another perspective;

[0042] Figure 10 This is a schematic diagram of the distributor of the present invention;

[0043] Figure 11 This is a schematic diagram of the dispenser of the present invention from another perspective;

[0044] Figure 12 This is a schematic diagram of the liquid suction mechanism of the present invention;

[0045] Figure 13 This is a schematic diagram of the suction head of the present invention;

[0046] Explanation of icon numbers:

[0047] PAGE gel recovery device 100; gel mixing and liquid suction mechanism 10; frame 11; gel mixing head placement seat 111; deep hole plate placement seat 112; heating device 1121; pressing plate 1122; suction head placement seat 113; waste chute 114; first waste box 115; second waste box 116; gel mixing head 12; liquid inlet 121; liquid inlet hole 1211; gel mixing section 122; connecting section 1221; liquid outlet 1222; liquid outlet hole 1222a; crushing section 1223; deep hole plate 13; suction head 14; filter Core 141; Agglomeration mechanism 15; First guide rail 151; First sliding seat 152; Second sliding seat 153; Distributor 154; Mounting groove 1541; Drain hole 1541a; Connector 1542; Electric gripper 155; Clamping part 1551; Anti-drip assembly 156; Connector 1561; Drain plate 1562; Liquid suction mechanism 16; Second guide rail 161; Third sliding seat 162; Fourth sliding seat 163; Pipette 164; Fusion liquid supply mechanism 20; Plunger pump 21; Reversing valve 22. Implementation

[0048] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0049] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indication will also change accordingly.

[0050] In this invention, unless otherwise explicitly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0051] Furthermore, in this invention, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. When the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed by this invention.

[0052] This invention proposes a PAGE gel recovery device 100.

[0053] To better understand the above technical solutions, the following will provide a detailed explanation of the technical solutions in conjunction with the accompanying drawings and specific implementation methods.

[0054] In embodiments of the present invention, such as Figure 1 , Figure 2 and Figure 13 As shown, the PAGE gel recovery device 100 includes a mixing and aspiration mechanism 10 and a fusion solution supply mechanism 20 arranged adjacent to each other. The mixing and aspiration mechanism 10 includes a frame 11, a mixing head 12, a deep well plate 13, a suction tip 14, a mixing mechanism 15, and a suction mechanism 16. The frame 11 has a worktable, and the worktable is provided with a mixing head placement seat 111, a deep well plate placement seat 112, and a suction tip placement seat 113 in sequence along the X-axis. The deep well plate placement seat 112 has multiple working areas along the X-axis, and one working area is provided with a heating device 1121 for heating the deep well plate 13; the mixing head 12, mixing... Head 12 is placed in mixing head placement seat 111; deep hole plate 13 is placed in deep hole plate placement seat 112; suction head 14 is placed in suction head placement seat 113, and the front end of suction head 14 has filter element 141; mixing mechanism 15 is used for mixing and adding liquid, and mixing mechanism 15 is connected to fusion liquid supply mechanism 20 through a hose. Mixing mechanism 15 is movably mounted on frame 11. Mixing mechanism 15 grabs mixing head 12 and moves it to the position of deep hole plate 13 for mixing or adding liquid; liquid suction mechanism 16 is movably mounted on frame 11. Liquid suction mechanism 16 is used to transfer liquid from one deep hole plate 13 to another deep hole plate 13.

[0055] Understandably, the mixing and liquid aspiration mechanism 10 is used to complete the mixing, heating, and pipetting processes. The fusion solution supply mechanism 20 provides the fusion solution during the mixing process. The mixing head placement seat 111 is used to place multiple mixing heads 12. Multiple working areas of the deep well plate placement seat 112 are respectively placed with deep well plates 13. The pipette tip placement seat 113 is used to place multiple pipette tips 14. The deep well plate placement seat 112 of the present invention has four working areas: a reagent working area, a mixing working area, a sterilization working area, and a pipetting working area. A heating device 1121 is provided at the position of the mixing working area. Each working area is equipped with a deep well plate 13. The deep well plate 13 in the reagent working area contains ethanol or other reagents, which are added to the deep well plates 13 in other working areas according to experimental needs. The mixing working area is used to complete the mixing, liquid addition, and heating processes in the gel recovery process. The sterilization working area is used to complete the cleaning and sterilization of the pipette tips 14. The well plate 13 contains a fusion solution, which is the same as the fusion solution supplied by the fusion solution supply mechanism 20. After the aspiration mechanism 16 draws the solution from the well plate 13 in the mixing work area, it moves to the sterilization work area and inserts the pipette tip 14 into the well plate 13 for cleaning. The filter element 141 of the pipette tip 14 is a polyethylene filter element, which can effectively filter impurities in the solution to obtain a relatively pure recovery solution. The pipetting work area is used to collect the recovered solution. After the pipette tip 14 is cleaned in the sterilization work area, the aspiration mechanism 16 transfers the collected solution into the well plate 13 in the pipetting work area.

[0056] The frame 11 of the present invention includes a frame, a protective cover, and a movable protective door. The frame and the protective cover form a working chamber with a front opening. The protective door is hinged to the top of the frame and the front opening is opened or closed by a nitrogen spring. The frame 11 has a waste collection assembly near the tamping head placement seat 111. The waste collection assembly includes a waste chute 114 and a first waste box 115. The first waste box 115 is located outside the working chamber. The waste chute 114 is inclined, with its top inside the working chamber and its bottom extending out of the working chamber and receiving the first waste box 115. The tamping mechanism 15 moves the used tamping head 12 to the position of the waste chute 114 and releases it, causing the tamping head 12 to fall along the chute into the first waste box 115 below. Furthermore, the frame 11 also includes an exhaust system for ventilation within the working chamber. Furthermore, the workbench is also equipped with a second waste box 116, which is located near the suction head placement seat 113 and is used to collect used suction heads 14.

[0057] The invention is implemented as follows: the cut gel is manually placed into multiple square holes in the deep-hole plate 13 of the mixing work area. The mixing mechanism 15 moves to the position of the mixing head placement seat 111, grabs one of the mixing heads 12 and moves it to the position above the deep-hole plate 13. The mixing head 12 extends into the square hole and moves vertically to simulate the mixing action. During the mixing process, the mixing mechanism 15 simultaneously adds liquid into the square hole. After each square hole of the deep-hole plate 13 has been mixed, the heating device 1121 heats the deep-hole plate 13 of the mixing work area for about 2 hours. Heating can fully dissolve the gel and improve the recovery rate. The mixing mechanism 15 moves the used mixing head 12 to the position of the waste chute 114 and releases it, so that the mixing head 12 falls into the first waste box 115 below along the direction of the chute. After heating is complete, the aspiration mechanism 16 first moves to the tip holder 113 to pick up the tip 14. The aspiration mechanism 16 then moves the tip 14 to the deep well plate 13 of the mixing zone to aspirate the solution. It then moves to the sterilization zone, allowing the tip 14 to penetrate the deep well plate 13 for sterilization and cleaning. This step cleans the outside and tip of the tip 14, preventing impurities from entering the deep well plate 13 of the pipetting zone. After cleaning, the aspiration mechanism 16 moves the tip 14 to the pipetting zone to transfer the aspirated solution into the deep well plate 13. This process constitutes the first recovery. In some DNA recovery processes... The process also includes a second recycling step. Specifically, the liquid aspiration mechanism 16 moves the pipette tip 14 to the sterilization work area to aspirate the fusion solution. The fusion solution is then transferred to the deep-hole plate 13 in the mixing work area. Since the mixing process was already performed in the first recycling step, it is not necessary to perform mixing again in this step. The heating device 1121 heats the deep-hole plate 13 in the mixing work area for about 2 hours. After heating is completed, the cleaning and pipetting processes of the first recycling step are repeated to transfer the solution to the deep-hole plate 13 in the pipetting work area. The liquid aspiration mechanism 16 moves the pipette tip 14 to the position of the second waste box 116 and discards the pipette tip 14 into the second waste box 116.

[0058] The technical solution of this invention includes a mixing and liquid aspiration mechanism 10 and a fusion liquid supply mechanism 20. The mixing and liquid aspiration mechanism 10 includes a frame 11, a mixing head 12, a deep well plate 13, a suction head 14, a mixing mechanism 15, and a liquid aspiration mechanism 16. There are multiple deep well plates 13, which are placed on a deep well plate placement seat 112. The mixing mechanism 15 grabs the mixing head 12 and moves it to the position of the deep well plate 13 to mix the adhesive or add liquid. The fusion liquid in the deep well plate 13 is heated by a heating device 1121. The liquid aspiration mechanism 16 is used to transfer the liquid from one deep well plate 13 to another. Because it adopts automatic liquid supply, automatic mixing, automatic heating, and automatic liquid transfer, it effectively solves the technical problem of low efficiency in manual operation in the prior art, and thus achieves the technical effect of improving efficiency and being suitable for large-scale testing.

[0059] In embodiments of the present invention, such as Figure 3 As shown, the mixing head placement seat 111 has N first stations along the Y-axis, each working area has N second stations along the Y-axis, and the suction head placement seat 113 has N third stations along the Y-axis. The first, second, and third stations are in a one-to-one correspondence. By adding multiple stations, efficiency is improved to meet batch testing requirements.

[0060] In embodiments of the present invention, such as Figure 6 , Figure 7 , Figure 8 and Figure 9 As shown, the mixing mechanism 15 includes a first guide rail 151, a first sliding seat 152, a second sliding seat 153, a distributor 154, and an electric gripper 155. The first guide rail 151 slides in cooperation with the frame 11 in the X-axis direction under the drive of a first power device; the first sliding seat 152 slides in cooperation with the first guide rail 151 in the Y-axis direction under the drive of a second power device; the second sliding seat 153 slides in cooperation with the first sliding seat 152 in the Z-axis direction under the drive of a third power device; and the distributor 154 connects to the machine via a flexible hose. The fusion liquid supply mechanism 20 is connected, and the distributor 154 is connected to the second sliding seat 153 through a connecting rod. The bottom of the distributor 154 is provided with a mounting groove 1541. The mixing head 12 is located in the mounting groove 1541 and is connected to the distributor 154. The mixing head 12 has a liquid outlet hole 1222a for discharging liquid. The electric gripper 155 is used to hold the mixing head 12. The electric gripper 155 is located in the second sliding seat 153. The electric gripper 155 has two clamping parts 1551, which are located on opposite sides of the mounting groove 1541.

[0061] In this invention, the first power device is a gear and rack transmission mechanism. The frame 11 has crossbeams on the front and rear sides, and racks are provided on the crossbeams. The two ends of the first guide rail 151 are provided with gears driven by a motor. The gears mesh with the racks, and the first guide rail 151 slides in the X-axis direction through the drive of the motor. The second and third power devices are both belt transmission mechanisms. Both the rack and rack transmission mechanism and the belt transmission mechanism are common mechanical transmission structures, and will not be described in detail here. The fusion liquid supply mechanism 20 supplies liquid to the distributor 154 via a plunger pump 21. Specifically, a storage tank containing fusion liquid is provided near the fusion liquid supply mechanism 20. The fusion liquid supply mechanism 20 also includes a reversing valve 22, which is connected between the plunger pump 21 and the distributor 154. The reversing valve 22 switches the pipeline. When the pipeline between the plunger pump 21 and the storage tank is connected, the pipeline between the plunger pump 21 and the distributor 154 is closed to achieve liquid suction. When the pipeline between the plunger pump 21 and the storage tank is closed, the pipeline between the plunger pump 21 and the distributor 154 is opened to achieve liquid spraying. The fusion liquid from the distributor 154 enters the mixing head 12 and exits from the liquid outlet 1222a of the mixing head 12 to achieve liquid discharge. The connection between the distributor 154 and the mixing head 12 can be tightly fitted by a sealing ring. When the mixing mechanism 15 moves to the position of the mixing head placement seat 111, the mounting groove 1541 is vertically aligned with any one of the mixing heads 12. The third power device drives the second sliding seat 153 to move downward, so that the top of the mixing head 12 is tightly fitted with the bottom of the mounting groove 1541. Then, the electric gripper 155 drives the two clamping parts 1551 to move towards each other to clamp and fix the mixing head 12.

[0062] In embodiments of the present invention, such as Figure 10 and Figure 11 As shown, the distributor 154 is provided with multiple connectors 1542 connected to the fusion liquid supply mechanism 20. The bottom of the mounting groove 1541 is provided with multiple sets of drain ports to correspond to the multiple connectors 1542. Each set of drain ports includes multiple drain holes 1541a. The top of the mixing head 12 is provided with multiple sets of inlets to correspond to the multiple drain ports. Each set of inlets includes multiple inlet holes 1211. The number of inlet holes 1211 and the number of drain holes 1541a are the same and their positions correspond. The outlet hole 1222a is connected to the inlet hole 1211.

[0063] Understandably, the fusion liquid supply mechanism 20 supplies liquid to the distributor 154 through the plunger pump 21. The plunger pump 21 and the connector 1542 can be in a one-to-one correspondence or a one-to-many relationship. That is, the number of plunger pumps 21 can be the same as the number of connectors 1542, or one plunger pump 21 can correspond to multiple connectors 1542. The present invention has a total of four plunger pumps 21 and eight connectors 1542. That is, one plunger pump 21 is connected to two connectors 1542 through a three-way connector and a hose. The bottom of the mounting groove 1541 is provided with eight sets of liquid dispensing ports. Each set of liquid dispensing ports is provided with six liquid dispensing holes 1541a. The diameter of the liquid dispensing holes 1541a is smaller than the inner diameter of the hose. By diverting the flow, the discharge of the fusion liquid can be made more uniform. The distributor 154 has injection nozzles at the corresponding drain holes 1541a. The injection nozzles act as diverters, so that the fusion liquid entering the connector 1542 can be evenly distributed to multiple drain holes 1541a, thereby achieving uniform liquid discharge from the mixing head 12.

[0064] In embodiments of the present invention, such as Figure 4 and Figure 5 As shown, the mixing head 12 includes a liquid inlet 121 and a mixing part 122 connected to each other. Multiple sets of liquid inlets are provided in the liquid inlet 121, and the mixing part 122 is provided at the bottom of the liquid inlet 121. The mixing part 122 has multiple parts and each corresponds to multiple sets of liquid inlets. The liquid outlet holes 1222a are provided on the four sides of the mixing part 122.

[0065] In this invention, a positioning groove is provided at the top center of the liquid inlet 121, and snap-fit ​​grooves are provided on opposite sides of the liquid inlet 121. A positioning part that mates with the positioning groove is provided at the center of the mounting groove 1541. A snap-fit ​​part that mates with the snap-fit ​​groove is provided in the clamping part 1551. The snap-fit ​​groove has a first inclined surface, and the snap-fit ​​part has a second inclined surface. When the clamping part 1551 clamps the tamping head 12, the second inclined surface abuts against the first inclined surface and provides upward support, so that the top of the tamping head 12 fits tightly against the bottom of the mounting groove 1541. To facilitate the assembly of the tamping head 12, a chamfer is also provided at the top of the mounting groove 1541. The liquid outlet 1222a has four sides of the tamping part 122, which can make the liquid discharge more uniform. The number of liquid outlets 1222a on different sides can be the same or different.

[0066] In embodiments of the present invention, such as Figure 4 and Figure 5 As shown, the mortar mixing section 122 includes a connecting section 1221, a liquid outlet section 1222 and a mortar mixing section 1223 connected in sequence. The connecting section 1221 is connected to the bottom of the liquid inlet section 121. The liquid outlet holes 1222a are provided on the four sides of the liquid outlet section 1222. The sides and bottom of the mortar mixing section 1223 are provided with mortar mixing grooves.

[0067] Understandably, gels are sticky and tend to adhere to the outer surface of the crushing section 1223 and the inner wall of the square hole during the crushing process. Therefore, in this invention, the liquid outlet holes 1222a are arranged on the four sides of the liquid outlet section 1222, which not only serve to add liquid but also to clean the crushing section 1223 and the square hole. The crushing grooves located on the outer surface of the crushing section 1223 are arc-shaped grooves and are arranged along the length of the crushing section 1223. The crushing grooves located at the bottom of the crushing section 1223 are circular grooves and are evenly distributed at the bottom of the crushing section 1223.

[0068] In an embodiment of the present invention, the length of the crushing part 1223 is defined as L1 and the width as D1, and the deep hole plate 13 has a plurality of square holes, the length of the square holes is defined as L2 and the width as D2, L1 < L2 and D1 < D2.

[0069] During the crushing process, the tamping mechanism 15 drives the tamping head 12 to move up and down, back and forth, and left and right inside the square hole. The back and forth and left and right movements allow the tamping head 12 to approach any inner wall of the square hole. The up and down movement simulates the tamping action and simultaneously scrapes off the gel adhering to any inner wall, allowing more gel to react with the fusion liquid and improving the recovery rate. Because the length of the tamping section 1223 is less than the length of the square hole, and the width of the tamping section 1223 is also less than the width of the square hole, it ensures that the tamping section 1223 can move back and forth and left and right within the square hole. Furthermore, the deep hole plate 13 is injection molded, and each square hole has a demolding angle for easy demolding. Therefore, the tamping section 122 of this invention also adjusts its taper according to the inclination angle of the square hole to better perform the tamping or scraping action.

[0070] In embodiments of the present invention, such as Figure 8 and Figure 9 As shown, the mortar mixing mechanism 15 also includes an anti-drip component 156, which includes a connecting seat 1561 and a drip-collecting plate 1562. The connecting seat 1561 is disposed on the first sliding seat 152. The drip-collecting plate 1562 slides horizontally with the connecting seat 1561 under the drive of the fourth power device.

[0071] Understandably, after the mixing head 12 completes the mixing process, the mixing mechanism 15 moves the used mixing head 12 to the position of the waste chute 114 and releases it, causing the mixing head 12 to fall into the first waste box 115 below along the direction of the chute. Since some fusion liquid remains on the surface of the mixing head 12, an anti-drip component 156 is provided to prevent the fusion liquid from dripping during the movement of the mixing head 12. In this invention, the fourth power device is a gear and rack transmission mechanism, the specific structure of which will not be described in detail. After the mixing is completed, the mixing mechanism 15 drives the mixing head 12 to slide upward, and at the same time, the fourth power device drives the dripping plate 1562 to slide horizontally to the position below the mixing head 12 to catch the dripping fusion liquid.

[0072] In embodiments of the present invention, such as Figure 3 As shown, the working area for tamping is defined as the tamping working area. The heating device 1121 is located in the tamping working area. The heating device 1121 is provided with a heating groove for positioning the deep hole plate 13. The top of the heating device 1121 is detachably provided with a pressing plate 1122.

[0073] In this invention, the heating device 1121 specifically includes a heat insulation plate, heating aluminum blocks, a heat insulation section, and pressure strips. The heat insulation plate has multiple placement slots, and multiple heating aluminum blocks are placed in the multiple placement slots respectively, with the top of the heating aluminum blocks flush with the top of the heat insulation plate. The heat insulation section is placed on top of the heat insulation plate and is composed of multiple limiting frames, each corresponding to a multiple heating aluminum block, to limit the placement of the deep hole plate 13. There are two pressure strips, located on opposite sides of the top of the heat insulation section. The pressure strips are equipped with positioning pins, and the pressure plate 1122 is equipped with corresponding positioning holes. The positioning pins and positioning holes can limit the placement of the pressure plate 1122. The deep hole plate 13 has positioning steps. When the pressure plate 1122 is installed, the bottom of the pressure plate 1122 abuts against the positioning steps, pressing the deep hole plate 13 to ensure that the deep hole plate 13 does not shake during the adhesive mixing process.

[0074] In embodiments of the present invention, such as Figure 12 As shown, the liquid aspiration mechanism 16 includes a second guide rail 161, a third sliding seat 162, a fourth sliding seat 163, and a pipette 164. The second guide rail 161 is slidably engaged with the frame 11 in the X-axis direction under the drive of a fifth power device; the third sliding seat 162 is slidably engaged with the second guide rail 161 in the Y-axis direction under the drive of a sixth power device; the fourth sliding seat 163 is slidably engaged with the third sliding seat 162 in the Z-axis direction under the drive of a seventh power device; and the pipette 164 is disposed on the fourth sliding seat 163.

[0075] In this invention, the fifth power device is the same as the first power device, both being gear and rack transmission mechanisms. The two ends of the second guide rail 161 are equipped with gears driven by a motor. The gears mesh with the rack, and the motor drives the second guide rail 161 to slide in the X-axis direction. The sixth and seventh power devices are both belt transmission mechanisms. Both rack and rack transmission mechanisms and belt transmission mechanisms are common mechanical transmission structures and will not be described in detail here. The liquid suction mechanism 16 drives the suction head 14 to move up and down, left and right, and back and forth through the three power devices. When the suction head 14 is suctioning liquid in the deep hole plate 13 of the gel-mixing working area, because there is gel in the square hole, to avoid insufficient liquid suction due to suctioning at the same position, the liquid suction mechanism 16 will drive the suction head 14 to move back and forth and left and right to absorb more solution.

[0076] The above description is merely an optional embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A PAGE gel recovery device, characterized in that, The PAGE gel recovery device includes an adjacently arranged gel mixing and liquid aspiration mechanism and a fusion solution supply mechanism. The gel mixing and liquid aspiration mechanism includes: The frame has a worktable, and the worktable is provided with a tamping head placement seat, a deep hole plate placement seat and a suction head placement seat in sequence along the X-axis direction. The deep hole plate placement seat is provided with multiple working areas along the X-axis direction, and one of the working areas is provided with a heating device for heating the deep hole plate. A tamping head, wherein the tamping head is placed in the tamping head placement seat; A deep hole plate, wherein the deep hole plate is placed in a deep hole plate placement seat; A suction head, which is placed in a suction head holder, has a filter element at its front end; The mixing mechanism is used for mixing and adding liquid. The mixing mechanism is connected to the fusion liquid supply mechanism through a hose. The mixing mechanism is movably mounted on the frame. The mixing mechanism grabs the mixing head and moves it to the position of the deep hole plate to mix or add liquid. A liquid suction mechanism, movably mounted on the frame, is used to transfer liquid from one deep-hole plate to another deep-hole plate; The adhesive mixing mechanism includes: The first guide rail slides in the X-axis direction with the frame under the drive of the first power device; The first sliding seat slides in the Y-axis direction with the first guide rail under the drive of the second power device. The second sliding seat is slidably engaged with the first sliding seat in the Z-axis direction under the drive of the third power device; The dispenser is connected to the fusion liquid supply mechanism via a hose and is connected to the second sliding seat via a connecting rod. The bottom of the dispenser is provided with a mounting groove, and the mixing head is located in the mounting groove and communicates with the dispenser. The mixing head has a liquid outlet hole for discharging liquid. An electric gripper is used to hold the tamping head. The electric gripper is disposed on the second sliding seat and has two gripping parts located on opposite sides of the mounting groove.

2. The PAGE gel recovery device as described in claim 1, characterized in that, The mixing head placement seat has N first workstations along the Y-axis direction, each work area has N second workstations along the Y-axis direction, and the suction head placement seat has N third workstations along the Y-axis direction. The first workstations, second workstations, and third workstations are in a one-to-one correspondence.

3. The PAGE gel recovery device as described in claim 1, characterized in that, The distributor is provided with multiple connectors that are connected to the fusion liquid supply mechanism. The bottom of the mounting groove is provided with multiple sets of drain ports to correspond to the multiple connectors. Each set of drain ports includes multiple drain holes. The top of the mixing head is provided with multiple sets of inlets to correspond to the multiple drain ports. Each set of inlets includes multiple inlet holes. The number of inlet holes and the number of drain holes are the same and their positions correspond. The outlet hole is connected to the inlet hole.

4. The PAGE gel recovery device as described in claim 3, characterized in that, The mixing head includes a liquid inlet and a mixing part connected to each other. Multiple sets of liquid inlets are provided in the liquid inlet. The mixing part is located at the bottom of the liquid inlet. The mixing part has multiple mixing parts, each corresponding to a set of liquid inlets. The liquid outlet is located on the four sides of the mixing part.

5. The PAGE gel recovery device as described in claim 4, characterized in that, The mixing section includes a connecting section, a liquid outlet section, and a mixing section connected in sequence. The connecting section is connected to the bottom of the liquid inlet section. The liquid outlet holes are located on the four sides of the liquid outlet section. The sides and bottom of the mixing section are provided with mixing grooves.

6. The PAGE gel recovery device as described in claim 5, characterized in that, The length of the crushing section is defined as L1 and the width as D1. The deep hole plate has multiple square holes, and the length of the square holes is defined as L2 and the width as D2, where L1 < L2 and D1 < D2.

7. The PAGE gel recovery device as described in claim 1, characterized in that, The adhesive mixing mechanism further includes an anti-drip component, which includes: Connecting seat, the connecting seat being disposed on the first sliding seat; A drain plate, which slides horizontally with the connecting seat under the drive of the fourth power device.

8. The PAGE gel recovery device as described in claim 1, characterized in that, The working area for mixing the adhesive is defined as the mixing working area. The heating device is located in the mixing working area. The heating device is provided with a heating groove for positioning the deep hole plate. A clamping plate is detachably provided on the top of the heating device.

9. The PAGE gel recovery device as described in claim 1, characterized in that, The liquid suction mechanism includes: The second guide rail slides in the X-axis direction with the frame under the drive of the fifth power device; The third sliding seat is slidably engaged with the second guide rail in the Y-axis direction under the drive of the sixth power device; The fourth sliding seat is slidably engaged with the third sliding seat in the Z-axis direction under the drive of the seventh power device; A pipette, wherein the pipette is disposed on the fourth sliding seat.

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