Single-chain binding protein mutant with increased thermal stability, nucleotide sequence and application
CN116693627BActive Publication Date: 2026-08-28HANGZHOU ZHONGCE BIO SCI&TECH CO LTD
Patent Information
- Application Number
- CN202310269776.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-03-16
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2043-03-16
AI Technical Summary
Technical Problem
[0003]本发明提供了热稳定性增加的单链结合蛋白突变体、核苷酸序列及应用，旨在解决现有的单链结合蛋白热稳定性较差的问题
Benefits of technology
[0018]本发明的单链结合蛋白突变体具有更好的的热稳定性，与野生型蛋白需保存在﹣20℃条件下不同，本发明的单链结合蛋白突变体可实现常温存储运输。
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Figure CN116693627B_ABST
Abstract
The application provides a single-chain binding protein mutant with increased thermal stability, a nucleotide sequence and an application, and aims to solve the problem of poor thermal stability of existing single-chain binding protein mutants. The application provides a single-chain binding protein mutant with increased thermal stability, and the amino acid sequence of the single-chain binding protein mutant is shown in SEQ ID NO. 1. The single-chain binding protein mutant has better thermal stability, and unlike the wild-type protein which needs to be stored at-20 DEG C, the single-chain binding protein mutant can be stored and transported at room temperature. In addition, under the condition of constant temperature amplification reaction at 60-65 DEG C, the mutant protein has better reaction performance compared with the wild-type.
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Citation Information
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