A method for rapidly cultivating P5 shrimp larvae

Abstract

This invention provides a method for rapidly cultivating P5 shrimp larvae, comprising the following steps: transferring nauplii into a larval rearing pond for cultivation; after more than 80% of the nauplii have developed into zoea larvae, feeding them artificial feed for zoea larvae; after more than 80% of the zoea larvae have developed into mysid larvae, feeding them feed for Artemia nauplii and mysid larvae; after more than 80% of the mysid larvae have metamorphosed into shrimp larvae, feeding them rotifers and shrimp flakes; the artificial feed for zoea larvae includes: 45-55 parts of Schizochytrium powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil. The artificial feed for mysid larvae includes: 40-44 parts of shrimp flakes, 12-16 parts of tubifex worm powder, 5-10 parts of mannan oligosaccharide, 10-14 parts of scabra oil, and 20-30 parts of *Eupatorium fortunei* oil. This invention shortens the development time while obtaining high-quality P5 shrimp larvae, and throughout the entire cultivation process, the shrimp larvae mortality rate is low and the seedling survival rate is high.

Description

Technical Field

[0001] This invention relates to the field of shrimp seedling technology, specifically to a method for rapidly cultivating P5 shrimp seedlings. Background Technology

[0002] The growth and development of shrimp is divided into stages such as fertilized egg, nauplius, zoea, mysid larva, juvenile shrimp, and adult shrimp. P5 shrimp larvae refer to 5-day-old larvae, also known as juvenile shrimp. P5 shrimp larvae begin to live on the bottom or against the wall, consuming larger animal feed, and are basically ready to leave the shrimp hatchery and enter the outdoor shrimp pond for culture.

[0003] The Pacific white shrimp (Litopenaeus vannamei) is a large tropical shrimp, rich in nutrients and delicious in taste, widely loved by consumers. It has become a widely promoted shrimp farming species in my country. After hatching, the fertilized eggs of the Pacific white shrimp develop into nauplius larvae. After about 48 hours, the nauplius larvae develop into zoea larvae, and after about 4 days, they enter the mysid larval stage, beginning to show the appearance of small shrimp. After another 4 days, the mysid larvae metamorphose into juvenile shrimp, which can swim horizontally and are basically similar in appearance to adult shrimp. The development from nauplius larvae to P5 shrimp larvae involves multiple metamorphoses, taking about 14-15 days. For large-scale production, this 15-day larval rearing cycle is relatively long and cannot meet the requirements for rapid shrimp larval production. Currently, there is no effective method for rapidly cultivating P5 shrimp larvae. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the present invention provides a method for rapidly cultivating P5 shrimp larvae.

[0005] The technical solution of this invention is as follows:

[0006] A method for rapidly cultivating P5 shrimp larvae includes the following steps:

[0007] Nauplius larvae were transferred to larval rearing tanks for cultivation, with a rearing density controlled at 100,000-150,000 larvae / m². 3 Water temperature 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, water depth 0.8-0.9m;

[0008] After more than 80% of the nauplii develop into daphnia, artificial feed for daphnia is provided.

[0009] More than 80% of the daphnia larvae develop into mysid larvae and are then fed with the same food as Artemia nauplii and mysid larvae.

[0010] More than 80% of the mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp chips;

[0011] The artificial feed for daphnia larvae includes the following components by weight: 45-55 parts of schistocytic algae powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil.

[0012] Artificial feed for mysid shrimp larvae consists of the following components by weight: 40-44 parts shrimp chips, 12-16 parts tubifex worm powder, 5-10 parts mannan oligosaccharide, 10-14 parts cypermethrin oil, and 20-30 parts agastache oil.

[0013] Preferably, the artificial feed for Daphnia davidii larvae comprises the following components in parts by weight: 50 parts of Schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil.

[0014] Preferably, the artificial feed for mysid shrimp larvae comprises the following components in parts by weight: 40 parts shrimp chips, 16 parts tubifex worm powder, 5 parts mannan oligosaccharide, 14 parts saccharin oil, and 25 parts agastache oil.

[0015] Preferably, after more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 1.0-2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0016] Preferably, after more than 80% of the daphnia larvae develop into mysid larvae, they are fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii are fed at a rate of 8-15 nauplii per mysid per day, divided into 3-6 feedings. The mysid larvae are fed artificial feed 3-5 times per day, at a rate of 1.5-3.0 g / m³ each time. 3 First, feed the mysid shrimp larvae artificial feed, and then feed them Artemia nauplii larvae half an hour later, feeding them at intervals.

[0017] Preferably, after more than 80% of the mysid shrimp larvae metamorphose into baby shrimp, they are fed rotifers and shrimp meal. For baby shrimp (P1-P5), the diet consists mainly of rotifers, supplemented with shrimp meal. Shrimp meal is fed 6-8 times per day, at a rate of 2-3 g / m³ per feeding. 3 The amount of rotifers fed is 50-100 per juvenile shrimp per day, divided into 3-6 feedings.

[0018] Preferably, the shrimp larvae are Litopenaeus vannamei shrimp larvae.

[0019] Compared with the prior art, the beneficial results of the present invention are as follows:

[0020] This invention shortens the cultivation time for P5 shrimp larvae by more than 40 hours, achieving a larvae survival rate of over 90%. While shortening the development time, it obtains high-quality P5 shrimp larvae, and throughout the entire cultivation process, the larvae mortality rate is low, and the survival rate is high.

[0021] This invention enables large-scale cultivation, and the cultivation scheme is relatively simple, making it suitable for industrial implementation. Detailed Implementation

[0022] To enable those skilled in the art to better understand the technical content of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0023] Extraction method of perilla leaf oil / sharp rabbit lip flower oil / sai sunflower oil / peppermint oil / coix root oil: Take dried medicinal materials, crush them to obtain powder; add water at a material-to-liquid mass ratio of 1:20 and soak for 6 hours, then steam distill at 200℃ for 6 hours, and separate the oil and water to obtain perilla leaf oil / sharp rabbit lip flower oil / sai sunflower oil / peppermint oil / coix root oil.

[0024] Lagochilusleia canthus Fisch. (Lagochilusleia canthus Fisch., a plant in the Lamiaceae family) is the whole herb.

[0025] Perilla leaves: the leaves of Perilla frutescens, a plant belonging to the genus Perilla in the family Lamiaceae.

[0026] Malvastrum coromandelianum: The whole herb of Malvastrum coromandelianum, a plant in the Malvaceae family.

[0027] Peilan: The dried aerial parts of Eupatorium fortunei Turcz., a plant belonging to the genus Eupatorium in the family Asteraceae.

[0028] Job's tears root: the root of the grass Coix lacryma-jobi L.

[0029] Example 1:

[0030] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0031] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0032] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³.3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0033] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0034] Artificial feed for daphnia larvae: 50 parts of schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0035] Artificial feed for juvenile mysid shrimp: 40 parts shrimp chips, 16 parts tubifex powder, 5 parts mannan oligosaccharide, 14 parts saccharin oil, and 25 parts agastache oil are mixed, granulated, and sieved through a 150-mesh screen.

[0036] Example 2:

[0037] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0038] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 1.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0039] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 15 nauplii per mysid per day, divided into three feedings. The mysid larvae were given artificial feed five times a day, at a dose of 1.5 g / m³ each time. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0040] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 8 times per day, 2g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 100 per juvenile shrimp per day, divided into 3 feedings.

[0041] Artificial feed for daphnia larvae: 50 parts of schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0042] Artificial feed for juvenile mysid shrimp: 40 parts shrimp chips, 16 parts tubifex powder, 5 parts mannan oligosaccharide, 14 parts saccharin oil, and 25 parts agastache oil are mixed, granulated, and sieved through a 150-mesh screen.

[0043] Example 3:

[0044] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0045] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0046] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0047] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0048] Artificial feed for daphnia larvae: 55 parts of schizochytrium powder, 15 parts of soy milk, 15 parts of egg yolk, and 15 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0049] Artificial feed for juvenile mysid shrimp: 44 parts shrimp chips, 12 parts tubifex powder, 10 parts mannan oligosaccharide, 14 parts cypermethrin oil, and 20 parts agastache oil are mixed, granulated, and sieved through a 150-mesh sieve.

[0050] Example 4:

[0051] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0052] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0053] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0054] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0055] Artificial feed for daphnia larvae: 45 parts of schizochytrium powder, 27 parts of soy milk, 10 parts of egg yolk, and 18 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0056] Artificial feed for juvenile mysid shrimp: 40 parts shrimp chips, 15 parts tubifex powder, 5 parts mannan oligosaccharide, 10 parts saccharin oil, and 30 parts agastache oil are mixed, granulated, and sieved through a 150-mesh screen.

[0057] Example 5:

[0058] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0059] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0060] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0061] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0062] Artificial feed for Daphnia larvae: Mix 50 parts of Schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of perilla leaf oil, granulate, and sieve through a 250-mesh screen.

[0063] Artificial feed for juvenile mysid shrimp: 40 parts shrimp chips, 16 parts tubifex powder, 5 parts mannan oligosaccharide, 14 parts saccharin oil, and 25 parts agastache oil are mixed, granulated, and sieved through a 150-mesh screen.

[0064] Example 6:

[0065] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0066] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0067] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0068] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0069] Artificial feed for daphnia larvae: 50 parts of schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0070] Artificial feed for juvenile mysid shrimp: 40 parts shrimp chips, 16 parts tubifex powder, 5 parts mannan oligosaccharide, 14 parts coix root oil, and 25 parts agastache oil are mixed, granulated, and sieved through a 150-mesh sieve.

[0071] Example 7:

[0072] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0073] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0074] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0075] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0076] Artificial feed for daphnia larvae: 50 parts of schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil are mixed, granulated, and sieved through a 250-mesh sieve.

[0077] Artificial feed for mysid shrimp larvae: 40 parts shrimp chips, 16 parts tubifex powder, 5 parts mannan oligosaccharide, 14 parts saccharin oil, and 25 parts coix root oil are mixed, granulated, and sieved through a 150-mesh sieve.

[0078] Example 8:

[0079] After collecting and counting the nauplius larvae of Litopenaeus vannamei, they were transferred to larval rearing tanks with a height of 1.2m. The water was filtered and disinfected seawater, and the rearing density was controlled at 100,000-150,000 larvae / m². 3 The water temperature should be 27℃-29℃, pH 7.8-8.1, salinity 28‰-30‰, dissolved oxygen ≥5mg / L, and water depth 0.8-0.9m. One hour after the nauplius larvae are transferred into the rearing pond, cephalexin (1μL / L) should be administered once for each of the zoea larvae, mysid larvae, and postlarvae stages.

[0080] After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change 10-15 cm of water daily after the water level is full.

[0081] After over 80% of the zoea larvae developed into mysid larvae, they were fed with the same feed as Artemia nauplii and mysid larvae. The Artemia nauplii were fed 8 nauplii per mysid per day, divided into 6 feedings. The mysid larvae were given artificial feed 3 times a day, at a dose of 3.0 g / m³. 3 First feed the mysid shrimp larvae with artificial feed, then feed them Artemia nauplii half an hour later, and feed them at intervals.

[0082] Over 80% of mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp meal. Baby shrimp from P1 to P5 are primarily fed rotifers, supplemented with shrimp meal. Shrimp meal is fed 6 times per day, 3g / m² per feeding, sieved through an 80-mesh sieve. 3 The amount of rotifers fed is 50 per juvenile shrimp per day, divided into 6 feedings.

[0083] Artificial feed for Daphnia larvae: Mix 50 parts of Schizochytrium powder, 20 parts of soy milk, and 10 parts of egg yolk, granulate, and sieve through a 250-mesh sieve.

[0084] Artificial feed for mysid shrimp larvae: 40 parts shrimp chips, 16 parts tubifex powder, and 5 parts mannan oligosaccharide are mixed, granulated, and sieved through a 150-mesh screen.

[0085] The survival rate and development time of each of the above embodiments were statistically analyzed. At least three parallel experiments were conducted in each group. The survival rate refers to the percentage of nauplii that were raised to adult shrimp with a body length ≥0.6cm; the development time refers to the time interval between 90% of the nauplii developing into P5 shrimp with a body length of ≥0.6cm.

[0086] The difference between Example 5 and Example 1 was that the oil from *Hypericum perforatum* was replaced with perilla leaf oil; the difference between Example 6 and Example 1 was that the oil from *Cyperus rotundus* was replaced with coix root oil; the difference between Example 7 and Example 1 was that the oil from *Eupatorium fortunei* was replaced with coix root oil; Example 8 used the same method for cultivating P5 shrimp larvae as before the improvement. The results showed that the growth and development of shrimp in groups 1-4 of Examples were better than in the other groups. The development time of high-quality P5 shrimp larvae was significantly shorter (p < 0.01) than in the other groups, shortened by more than 40 hours.

[0087] The seedling rate of Example 8 was the lowest, at around 50%, while the seedling rates of Examples 1-4 were the highest, exceeding 90%, significantly higher than that of Example 8. The seedling rates of all other groups were also significantly different from those of Examples 1-4 (p < 0.01).

[0088] The above results show that the present invention achieves high-quality P5 shrimp larvae while shortening the development time, and the shrimp larvae mortality rate is low and the seedling survival rate is high throughout the entire cultivation process.

[0089] Table 1

[0090] Group Seedling survival rate / % Development time / h Example 1 Group 92.4％ 210 Example 2 group 90.5％ 218 Example 3 Group 90.6％ 215 Example 4 group 93.3％ 216 Example 5 group 81.7％ 276 Example 6 group 75.0％ 259 Example 7 group 87.4％ 248 Example 8 group 50.8％ 298

[0091] The above description is only a partial embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for rapidly cultivating P5 shrimp larvae, characterized in that, The shrimp larvae are Litopenaeus vannamei shrimp larvae, and the method includes the following steps: Nauplius larvae were transferred to larval rearing tanks for cultivation, with a rearing density controlled at 100,000-150,000 larvae / m². 3 The water depth is 0.8-0.9m. After more than 80% of the nauplii develop into daphnia, artificial feed for daphnia is provided. More than 80% of the daphnia larvae develop into mysid larvae and are then fed artificial feed containing artichoke nauplius and mysid larvae. More than 80% of the mysid shrimp larvae metamorphose into baby shrimp and are then fed rotifers and shrimp chips; The artificial feed for Daphnia davidii larvae consists of the following components by weight: 45-55 parts of Schizochytrium powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil. Artificial feed for mysid shrimp larvae consists of the following components by weight: 40-44 parts shrimp chips, 12-16 parts tubifex worm powder, 5-10 parts mannan oligosaccharide, 10-14 parts cypermethrin oil, and 20-30 parts agastache oil.

2. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, The artificial feed for Daphnia davidii larvae consists of the following components by weight: 50 parts of Schizochytrium powder, 20 parts of soy milk, 10 parts of egg yolk, and 20 parts of *Gnaphalium affine* oil.

3. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, Artificial feed for mysid shrimp larvae consists of the following components by weight: 40 parts shrimp chips, 16 parts tubifex worm powder, 5 parts mannan oligosaccharide, 14 parts cypermethrin oil, and 25 parts agastache oil.

4. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, After more than 80% of the nauplii have developed into daphnia, artificial feed for daphnia is introduced, with a feeding amount of 1.0-2.0 g / m³ each time. 3 Raise the water level by 10-15 cm daily, and change the water by 10-15 cm daily after the water level is full.

5. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, After more than 80% of the zoea larvae develop into mysid larvae, they are fed with artificial feed containing Artemia nauplii and mysid larvae. The amount of Artemia nauplii is 8-15 per mysid larvae per day, divided into 3-6 feedings. The artificial feed for mysid larvae is given 3-5 times per day, at a dose of 1.5-3.0 g / m³ each time. 3 First, feed the mysid shrimp larvae artificial feed, and then feed them Artemia nauplii larvae half an hour later, feeding them at intervals.

6. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, After more than 80% of the mysid shrimp larvae metamorphose into baby shrimp, they are fed rotifers and shrimp flakes. The diet of baby shrimp from P1 to P5 is mainly rotifers, supplemented with shrimp flakes. The amount of shrimp flakes is 6-8 times / day, 2-3g / m² each time. 3 The amount of rotifers fed is 50-100 per juvenile shrimp per day, divided into 3-6 feedings.

7. The method for rapidly cultivating P5 shrimp larvae according to claim 1, characterized in that, Nauplii were transferred to a larval rearing tank for cultivation at a water temperature of 27℃-29℃, pH of 7.8-8.1, salinity of 28‰-30‰, and dissolved oxygen of ≥5mg / L.