A synergistic nano-microemulsion for rodent sterilants and its application

Abstract

The present invention discloses a synergistic nano microemulsion for rodent sterilants and its application. The synergistic nano microemulsion for rodent sterilants comprises span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, hydrogenated castor oil polyoxyethylene ether and water. The volume ratio of span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, hydrogenated castor oil polyoxyethylene ether and water is 10-20:20-50:20-50:20-50:20-50:1000-2000. The synergistic nano microemulsion for rodent sterilants used in the present invention reduces the dosage of rodent sterilants by about 50%, saves costs, reduces the environmental residues of the drug and reduces the risk to other non-target organisms.

Description

Technical Field

[0001] The present invention belongs to the technical field of rodent infertility agents, and particularly relates to a nanoemulsion for enhancing the efficacy of rodent infertility agents and its application. Background Art

[0002] Currently, controlling rodent pests mainly relies on chemical killing mainly based on anticoagulants. However, problems such as short control efficacy, easy rebound of the population, harm to natural enemies, environmental pollution, and drug resistance are becoming increasingly serious, and it has an adverse impact on non-target species and human safety. Since the late 1950s, Chinese scholars have carried out research on infertility technologies, and screened out infertility agents with application potential such as quinestrol, cabergoline, and chlormadinone acetate, and have conducted in-depth research on the physiological mechanism and infertility effect of infertility agents. However, whether it is the application of anticoagulants or infertility agents, the active ingredients need to be delivered through baits. The palatability of bait plays a key role in the efficacy. Baits with good palatability can improve the utilization rate of bait and reduce the environmental pollution caused by drugs. However, endocrine-disrupting infertility agents such as quinestrol have poor palatability. For example, Rattus losea and Berylmys berdmorei have low food intake for quinestrol baits prepared by the soaking method and the adhesion method respectively. On the other hand, endocrine-disrupting infertility agents are poorly soluble in water, and the prepared baits have problems such as uneven dispersion and attachment of drugs and low effective dose, resulting in waste and abuse of drugs. In practice, by changing the base bait components in bait preparation, adding auxiliaries, and changing the bait preparation method and other means, the palatability of bait is improved, the intake of bait by harmful rodents is increased, the penetration and adsorption of drugs are improved, and thus the effective dose of drugs in bait is increased. Commonly used pesticide penetration solubilizers such as dimethylformamide, but it has irritating effects on eyes, skin and respiratory tract. It can cause mild to moderate upper respiratory tract irritation symptoms. Contaminating the skin can cause burns of varying degrees. Liver damage is often prominent in acute poisoning, and its residue will cause environmental pollution and pose a safety hazard to non-target animals. Therefore, there is an urgent need to develop a highly efficient, low-toxic and environmentally friendly synergist to improve the utilization rate of infertility agents and meet the requirements of reducing the amount of pesticides and chemical fertilizers and increasing efficiency. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a nanoemulsion for enhancing the efficacy of rodent infertility agents and its application in the preparation of rodent bait materials. The nanoemulsion for enhancing the efficacy of rodent infertility agents used in the present invention reduces the dosage of rodent infertility agents by about 50%, saves costs, reduces the environmental residue of drugs, and reduces the risk to other non-target organisms.

[0004] The first object of the present invention is to provide a synergistic nano microemulsion for rodent sterilants, which comprises span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, hydrogenated castor oil polyoxyethylene ether and water. The volume ratio of span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, hydrogenated castor oil polyoxyethylene ether and water is 10-20:20-50:20-50:20-50:20-50:1000-2000.

[0005] Diethylene glycol is a colorless, odorless, transparent, hygroscopic viscous liquid with a pungent sweet taste, no corrosion, and low toxicity. Melting point: -6.5 °C; boiling point: 245 °C; freezing point: -10.45 °C; relative density: 1.1161; refractive index: 1.4472; flash point: 123.9 °C; it is easily soluble in water, alcohol, acetone, ether, ethylene glycol and other polar solvents, and insoluble in benzene and carbon tetrachloride. Its chemical properties are similar to those of ethylene glycol. It can be directly used as a natural gas dehydration desiccant, textile lubricant, softener, and solvent for nitrocellulose, resin, grease, printing ink, etc. It can also be used to prepare cleaning agents and as a dispersing solvent in other daily chemicals such as printing ink. The molecular structure of diethylene glycol contains two functional groups, ether bond and hydroxyl group, which endow it with unique physical and chemical properties. Therefore, various chemical products such as ethers, acids, esters, and amines can be prepared from diethylene glycol and are widely used in industries such as petrochemical, rubber, plastic, textile, coating, adhesive, and pharmaceutical industries, with very wide applications. The acute toxicity of diethylene glycol is relatively low, and the oral median lethal dose LD 50 >5000 mg / kg for rats. Utilizing the carrier characteristics of diethylene glycol, it can also be used as an additive for pesticides. Adding a small amount of diethylene glycol to certain pesticides helps the pesticides penetrate to the body surface to improve the drug efficacy.

[0006] Microemulsion is a clear and transparent dispersion system composed of three substances: aqueous phase, surfactant, and oil phase. It has good stability in thermodynamics, with a low viscosity, appearing transparent or translucent in appearance and being isotropic. The reason for the thermodynamic stability of the microemulsion is the combined action of surfactants and co-surfactants in the system. The particle size of the microemulsion is generally <100nm and it also has good applications in many fields. Currently, microemulsification technology is mainly applied in fields such as cosmetics, food, pesticides, and pharmaceuticals. Due to the problems of poor water solubility, poor oil solubility, and poor stability of high-content bioactive ingredient substances, their application in the development of pesticide systems is somewhat restricted. However, microemulsion provides a better channel for the utilization of these high-content bioactive ingredient substances. It can be used as a functional ingredient carrier to achieve the water solubility or oil solubility of the substance, improve their dispersibility and bioavailability. Currently, based on the research of microemulsion, we have also developed a more environmentally friendly surfactant-free microemulsion with more obvious advantages. In this paper, according to the characteristics of diethylene glycol, a nano-microemulsion system is constructed: span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, polyoxyethylene hydrogenated castor oil, and water.

[0007] The present invention uses diethylene glycol as a main synergistic ingredient, in combination with a microemulsion (span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, polyoxyethylene hydrogenated castor oil, and water) that acts with drugs. Through quantitative multi-step treatment, the preparation and application of a nano-microemulsion for synergizing a sterilant are realized, with a simple procedure and easy operation.

[0008] The second object of the present invention is to provide a preparation method for the above-mentioned nano-microemulsion for synergizing a rodent sterilant, including the following steps:

[0009] (1) Mix the formulated amounts of span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, polyoxyethylene hydrogenated castor oil, and diethylene glycol solution.

[0010] (2) Add water to the above solution for dilution, with the water temperature at 60 - 80°C; stir until completely dissolved to obtain a nano-microemulsion for synergizing a rodent sterilant.

[0011] The third object of the present invention is to provide a rodent bait material containing the above-mentioned nano-microemulsion for synergizing a rodent sterilant as an active ingredient.

[0012] The fourth object of the present invention is to provide the application of the nano-microemulsion for synergizing a rodent sterilant in the preparation of a rodent bait material.

[0013] Preferably, it includes the following steps:

[0014] (1) Add a rodent sterilant to the nano microemulsion for enhancing the efficacy of rodent sterilants and stir until uniform to form a sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilants.

[0015] (2) Add bait materials to the sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilants in step (1), stir evenly, and dry or bake to obtain rodent bait materials containing the nano microemulsion for enhancing the efficacy of rodent sterilants.

[0016] Preferably, the addition of the rodent sterilant is to add 5 - 20 g of the rodent sterilant per 1000 mL of the nano microemulsion for enhancing the efficacy of rodent sterilants; the addition of the bait materials is to add 40 - 500 kg of the bait materials per 1000 mL of the sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilants.

[0017] Preferably, the rodent sterilant is a fat-soluble sterilant or a water-soluble sterilant.

[0018] Preferably, the rodent sterilant is quinestrol, chlormadinone acetate or cabergoline.

[0019] Preferably, the bait materials are selected from one or more of paddy rice, wheat and corn.

[0020] The fifth object of the present invention is to provide the application of the above-mentioned nano microemulsion for enhancing the efficacy of rodent sterilants or the above-mentioned rodent bait materials in inhibiting rodent reproduction.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] 1. The present invention uses diethylene glycol as a main synergistic component, in combination with a microemulsion (span80, dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, polyoxyethylene hydrogenated castor oil and water) for the drug action. Through quantitative multi-step treatment, the preparation and application of the synergistic nano microemulsion are realized, and the procedure is simple and easy to operate.

[0023] 2. The present invention fills the blank in the actual application field of the existing nano microemulsion for enhancing the efficacy of rodent sterilants.

[0024] 3. It reduces the dosage of the rodent sterilant by about 50%, saves costs, reduces the environmental residue of the drug and reduces the risk to other non-target organisms.

[0025] 4. The feeding index of the bait materials is high, significantly improving the prevention and control effect of the sterilant and reducing the rodent reproduction rate. Detailed implementation manners

[0026] The following examples are further descriptions of the present invention rather than limitations on the present invention.

[0027] Unless otherwise specified, the experimental materials and reagents in the present invention are all conventional commercially available products in the technical field.

[0028] Example 1:

[0029] Measure 10 mL of span80, 20 mL of dodecyl diphenyl ether disulfonate, 40 mL of isomeric alcohol polyoxyethylene ether, 20 mL of hydrogenated castor oil polyoxyethylene ether, and 30 mL of diethylene glycol, add 1000 mL of water at 80 °C, stir and mix evenly to obtain a sterile agent synergistic nanoemulsion solution; weigh 400 kg of fresh paddy rice, add 1000 mL of the prepared sterile agent synergistic nanoemulsion solution, and add 400 kg of bait material per 1000 mL of the sterile agent synergistic nanoemulsion solution, stir until uniform, and dry to obtain a synergistic medicated bait.

[0030] The test adult Kunming mice (white mice) were purchased from the Guangdong Provincial Medical Experimental Animal Center. They were individually caged (30 cm × 15 cm × 20 cm), with sawdust as bedding, and water and food (standard mouse feed, produced by the Guangdong Provincial Medical Experimental Animal Center, SCXK(Yue)2013 - 0002) were supplied in sufficient quantities. The room temperature was 25 °C ± 2 °C, and the photoperiod was 12L:12D (08:00 - 20:00). The test mice were acclimatized for more than 2 months before being used in the experiment.

[0031] Select 16 healthy adult female and male mice each, and cage them individually. The female / male mice were randomly divided into 2 groups (blank cereal bait group, synergistic cereal bait group), with 8 female and male mice in each group. First, they were fed with pure paddy rice for one week, and then 20 g of blank cereal bait or synergistic cereal bait (i.e., synergistic medicated bait) was separately fed to each mouse in the two groups every day. The remaining bait was collected every 3 days, dried and weighed, and the experiment was repeated three times for a total of 9 days. Then, the female and male mice in the two groups were caged together for 7 days, and they were fed with pure paddy rice during the co - housing period. Record the pregnancy and litter - bearing situations of the female mice in each group. The reproductive situations of the female mice in the blank cereal bait and synergistic cereal bait groups are shown in Table 1:

[0032] Table 1

[0033]

[0034] It can be seen from Table 1 that the use of the synergistic cereal bait alone had no effect on the reproduction of white mice, and the separate sterile agent synergistic nanoemulsion solution had no effect.

[0035] Example 2:

[0036] Measure 10 mL of span80, 40 mL of dodecyl diphenyl ether disulfonate, 30 mL of isomeric alcohol polyoxyethylene ether, 20 mL of hydrogenated castor oil polyoxyethylene ether, and 50 mL of diethylene glycol. Add 1000 mL of water at 70 °C and stir to mix evenly to obtain a sterile agent synergistic nanoemulsion solution. Weigh 1000 mL of the evenly mixed sterile agent synergistic nanoemulsion solution, add 20 g of quinestrol, and add 20 g of rodent sterile agent per 1000 mL of the sterile agent synergistic nanoemulsion solution. Stir until completely dissolved to obtain a sterile agent solution containing the sterile agent synergistic nanoemulsion. Weigh 400 kg of fresh paddy rice, add 1000 mL of the prepared sterile agent solution containing the sterile agent synergistic nanoemulsion, and add 400 kg of bait material per 1000 mL of the sterile agent solution containing the sterile agent synergistic nanoemulsion. Stir until uniform and dry to obtain a synergistic quinestrol bait.

[0037] The preparation method of the ordinary quinestrol bait solution is to add 20 g of quinestrol per 1000 mL of water and stir until completely dissolved to obtain a quinestrol solution. Weigh 400 kg of fresh paddy rice, add 1000 mL of the prepared quinestrol solution, and add 400 kg of bait material per 1000 mL of the quinestrol solution. Stir until uniform and dry to obtain an ordinary quinestrol bait.

[0038] The test adult Kunming mice (white mice) were purchased from the Guangdong Provincial Medical Experimental Animal Center. Adult Apodemus agrarius were captured from the farmland of Fengcun, Zengcheng City, Guangdong Province. The two types of mice were separately housed in single cages (30 cm × 15 cm × 20 cm), with sawdust as bedding, and water and food (standard mouse feed, produced by the Guangdong Provincial Medical Experimental Animal Center, SCXK (Guangdong) 2013 - 0002) were supplied abundantly. The room temperature was 25 °C ± 2 °C, and the photoperiod was 12L:12D (08:00 - 20:00). The test mice were acclimated for more than 2 months before the experiment.

[0039] Select 24 healthy adult female and male white mice each, and house them in single cages. The female / male mice were randomly divided into 3 groups (blank grain bait group, synergistic quinestrol bait group, and ordinary quinestrol bait group), with 8 female and male mice in each group. First, feed them with pure paddy rice for one week. Thereafter, 20 g of blank grain bait, synergistic quinestrol bait, or ordinary quinestrol bait were respectively fed to each mouse in the three groups every day. The remaining bait was collected every 3 days, dried, and weighed. The experiment was repeated three times for a total of 9 days (the feeding test of the three baits for Apodemus agrarius was the same as that for Kunming mice). The food intake of Kunming mice and Apodemus agrarius for the three baits [(total bait input - remaining bait) / (experimental days)] is shown in Table 2.

[0040] The three groups of female and male white mice were respectively caged together for 7 days, and they were allowed to freely eat pure paddy rice during the caging period. Record the pregnancy and littering situations of female mice in each group. The reproductive situations of female mice in the blank grain bait group, synergistic quinestrol bait group, and ordinary quinestrol bait group are shown in Table 3.

[0041] Table 2

[0042]

[0043] Table 3

[0044]

[0045] It can be seen from Table 2 that the food intake of Rattus nitidus and Mus musculus for the synergistic ethinylestradiol bait is significantly higher than that for the ordinary ethinylestradiol bait. Diethylene glycol can increase the food intake of Rattus nitidus and Mus musculus for ethinylestradiol and improve the palatability of ethinylestradiol. From the results of Table 3, it can be seen that the reproductive rate of Mus musculus after feeding on the synergistic ethinylestradiol bait is lower than that of the blank cereal bait group and the ordinary ethinylestradiol bait group, only 30% of the control group and 50% of the ordinary ethinylestradiol bait group. The number of reproductive offspring and the number of surviving offspring are both lower than those of the blank cereal bait group and the ordinary ethinylestradiol bait group. It shows that diethylene glycol has a synergistic effect on the sterility effect of ethinylestradiol.

[0046] Example 3:

[0047] Measure 10 mL of span80, 20 mL of dodecyl diphenyl ether disulfonate, 40 mL of isomeric alcohol polyoxyethylene ether, 20 mL of hydrogenated castor oil polyoxyethylene ether, and 30 mL of diethylene glycol. Add 1000 mL of water at 80 °C and stir evenly to obtain a sterility agent synergistic nano-microemulsion solution; weigh 1000 mL of the mixed sterility agent synergistic nano-microemulsion solution, add 10 g of chlormadinone acetate, and add 10 g of chlormadinone acetate per 1000 mL of the sterility agent synergistic nano-microemulsion solution, stir until completely dissolved to obtain a synergistic chlormadinone acetate solution. Weigh 400 kg of fresh paddy rice, add 1000 mL of the prepared synergistic chlormadinone acetate solution, and add 400 kg of bait material per 1000 mL of the synergistic chlormadinone acetate solution, stir until uniform, and dry to obtain a synergistic chlormadinone acetate bait.

[0048] The preparation method of the ordinary chlormadinone acetate bait solution is to add 10 g of chlormadinone acetate per 1000 mL of water, stir until completely dissolved to obtain a chlormadinone acetate solution. Weigh 400 kg of fresh paddy rice, add 1000 mL of the prepared chlormadinone acetate solution, and add 400 kg of bait material per 1000 mL of the chlormadinone acetate solution, stir until uniform, and dry to obtain an ordinary chlormadinone acetate bait.

[0049] The test adult Kunming mice (white mice) were purchased from the Guangdong Provincial Medical Experimental Animal Center. The adult yellow-haired mice were captured from the farmland in Feng Village, Zengcheng City, Guangdong Province. The two types of mice were separately housed in single cages (30 cm × 15 cm × 20 cm), with sawdust as bedding, and water and food (standard mouse feed, produced by the Guangdong Provincial Medical Experimental Animal Center, SCXK (Guangdong) 2013-0002) were supplied in sufficient quantities. The room temperature was 25°C ± 2°C, and the photoperiod was 12L:12D (08:00-20:00). The test mice were acclimatized for more than 2 months before being used in the experiment.

[0050] Thirty healthy adult female and male white mice were selected. After being housed in single cages for one week, the female / male mice were randomly divided into 3 groups (blank cereal bait group, enhanced chlormadinone acetate bait group, and ordinary chlormadinone acetate bait group), with 10 female and male mice in each group. The female and male white mice in the three groups were caged together, and pure paddy rice was used for feeding during the co-housing period. After 7 days of co-housing, the female and male mice were separated and fed in single cages. Each mouse in the three groups was given 20 g of blank cereal bait, enhanced chlormadinone acetate bait, or ordinary chlormadinone acetate bait every day. The remaining bait was collected every 3 days, dried, and weighed. The experiment was repeated three times for a total of 9 days (the feeding test of the three baits for yellow-haired mice was the same as that for white mice), and then they were fed with pure paddy rice. The pregnancy and litter production of female mice in each group were recorded.

[0051] The food intakes of white mice and yellow-haired mice for the three baits are shown in Table 4. The reproductive situations of female white mice in the blank cereal bait group, enhanced chlormadinone acetate bait group, and ordinary chlormadinone acetate bait group are shown in Table 5.

[0052] Table 4

[0053]

[0054] Table 5

[0055]

[0056] It can be seen from Table 4 that the food intakes of yellow-haired mice and white mice for the enhanced chlormadinone acetate bait are higher than those for the ordinary chlormadinone acetate bait, and diethylene glycol can improve the palatability of chlormadinone acetate. From the results of Table 5, it can be seen that the reproductive rate of white mice after taking the chlormadinone acetate bait is lower than that of the blank cereal bait group, and the reproductive rate of the enhanced chlormadinone acetate bait group is only 30% of that of the control group and 50% of that of the ordinary chlormadinone acetate bait group. The number of reproductive offspring and the number of surviving offspring in the enhanced chlormadinone acetate bait group are both lower than those in the blank cereal bait group and the ordinary chlormadinone acetate bait group. It shows that diethylene glycol has a synergistic effect on the sterility effect of chlormadinone acetate.

[0057] Example 4:

[0058] Measure 20 mL of Span80, 50 mL of dodecyl diphenyl ether disulfonate, 50 mL of isomeric alcohol polyoxyethylene ether, 50 mL of hydrogenated castor oil polyoxyethylene ether, and 50 mL of diethylene glycol. Add 2000 mL of water at 60°C and stir to mix evenly to obtain a sterile agent synergistic nano-microemulsion solution. Weigh 2000 mL of the evenly mixed sterile agent synergistic nano-microemulsion solution, add 5 g of cabergoline, and add 5 g of cabergoline per 2000 mL of the sterile agent synergistic nano-microemulsion solution. Stir until completely dissolved to obtain a synergistic cabergoline solution. Weigh 500 kg of fresh wheat, add 2000 mL of the prepared synergistic cabergoline solution, and add 500 kg of bait material per 2000 mL of the synergistic cabergoline solution. Stir until uniform and dry to prepare a synergistic cabergoline bait.

[0059] The preparation method of a common cabergoline bait solution is to add 5 g of cabergoline per 2000 mL of water, stir until completely dissolved to obtain a cabergoline solution. Weigh 500 kg of fresh wheat, add 2000 mL of the prepared cabergoline solution, and add 500 kg of bait material per 2000 mL of the cabergoline solution. Stir until uniform and dry to prepare a common cabergoline bait.

[0060] The test adult Kunming mice (white mice) were purchased from the Guangdong Provincial Medical Experimental Animal Center. They were individually caged (30 cm × 15 cm × 20 cm), with sawdust as bedding, and water and food (standard mouse feed, produced by the Guangdong Provincial Medical Experimental Animal Center, SCXK (Guangdong) 2013-0002) were supplied in sufficient amounts. The room temperature was 25°C ± 2°C, and the photoperiod was 12L:12D (08:00-20:00). The test mice were acclimated for more than 2 months before being used in the experiment.

[0061] Select 30 healthy adult female and male white mice each. The female / male mice were randomly divided into 3 groups (blank wheat-based bait group, synergistic cabergoline bait group, and common cabergoline bait group), with 10 female and male mice in each group. The female and male white mice in the three groups were caged together. During the co-housing period, they were first fed with pure paddy rice. After 7 days of co-housing, the female and male mice were separated and individually caged. Thereafter, 20 g of blank wheat-based bait, synergistic cabergoline bait, or common cabergoline bait was fed to each mouse in the three groups every day. The experiment was repeated three times and lasted for a total of 9 days. Then, they were fed with normal feed. Record the pregnancy and littering situations of the female mice in each group. The reproductive situations of the female mice in the blank wheat-based bait group, synergistic cabergoline bait group, and common cabergoline bait group are shown in Table 6.

[0062] Table 6

[0063]

[0064] As can be seen from the results in Table 6, the reproductive rates of the mice fed with the ergoline medicated bait with enhanced efficacy are lower than those of the blank group and the group fed with the ordinary ergoline medicated bait. The reproductive rate of its female mice is only 30% of that of the blank group and 60% of that of the group fed with the ordinary ergoline medicated bait. The number of reproductive offspring and the number of surviving offspring individuals in the group fed with the ergoline medicated bait with enhanced efficacy are both lower than those in the blank wheat-based bait group and the group fed with the ordinary ergoline medicated bait. It shows that diethylene glycol has a synergistic effect on the sterility effect of ergoline.

[0065] Example 5:

[0066] Measure 10 mL of span80, 20 mL of dodecyl diphenyl ether disulfonate, 20 mL of isomeric alcohol polyoxyethylene ether, 20 mL of hydrogenated castor oil polyoxyethylene ether, and 50 mL of diethylene glycol. Add 1000 mL of water at 80 °C and stir evenly to obtain a nanoemulsion solution for enhancing the efficacy of the sterilant; Weigh 1000 mL of the nanoemulsion solution for enhancing the efficacy of the sterilant after mixing, add 20 g of quinestrol, and add 20 g of rodent sterilant per 1000 mL of the nanoemulsion solution for enhancing the efficacy of the sterilant, and stir until completely dissolved to obtain a sterilant solution containing the nanoemulsion for enhancing the efficacy of the sterilant. Weigh 40 kg of fresh paddy rice, add 1000 mL of the prepared sterilant solution containing the nanoemulsion for enhancing the efficacy of the sterilant, add 40 kg of bait material per 1000 mL of the sterilant solution containing the nanoemulsion for enhancing the efficacy of the sterilant, stir until uniform, and dry to obtain the medicated bait with enhanced efficacy of quinestrol.

[0067] The preparation method of the ordinary quinestrol medicated bait solution is to add 20 g of quinestrol to 1000 mL of water and stir until completely dissolved to obtain a quinestrol solution. Weigh 40 kg of fresh paddy rice, add 1000 mL of the prepared quinestrol solution, add 40 kg of bait material per 1000 mL of the quinestrol solution, stir until uniform, and dry to obtain the ordinary quinestrol medicated bait.

[0068] Determination of quinestrol content in paddy rice: Experimental instruments and reagents: Agilent 1260 high performance liquid chromatograph, variable wavelength ultraviolet detector; Quinestrol reference substance (National Institutes for Food and Drug Control, batch number: 100048 - 201503, for content determination, calculated by C 25 H 32 O2, content is 98.6%); Methanol and absolute ethanol are chromatographically pure, and other reagents are all analytically pure. Chromatographic column: Agilent C18 (250 mm × 4.6 mm, 5 μm); Mobile phase: methanol - water (90∶10); Flow rate: 1.0 mL / min; Detection wavelength: 279 nm; Injection volume: 20 μL; Column temperature: 30 °C.

[0069] Weigh the quinestrol standard product and prepare quinestrol standard working solutions with concentrations of 10, 20, 30, 40, 50, and 60 μg / mL using a methanol solution. Respectively, take 20 μL of the quinestrol standard working solutions of 10, 20, 30, 40, 50, and 60 μg / mL for injection analysis. Using the injection volume of the standard product as the abscissa X and the chromatographic peak area as the ordinate Y, plot the standard curve to obtain the regression equation: Y = 5.612X - 3.02 (R 2 = 0.9999). Weigh 10 g of each of the ordinary quinestrol bait and the synergistic quinestrol bait in 3 portions. Separate the bait (paddy) into brown rice (ground brown rice) and rice husks. Dissolve it repeatedly with a methanol solution, filter through a 0.22 μm microporous filter membrane, and perform the determination on the machine (recovery rate > 90%). Calculate the quinestrol content in the brown rice and rice husks of the ordinary quinestrol bait and the synergistic quinestrol bait respectively (see Table 7).

[0070] Table 7

[0071]

[0072] It can be seen from the results in Table 7 that the drug content in the rice husks of the ordinary quinestrol bait accounts for 91.7% of the total drug amount, and the drug content in the brown rice accounts for 8.3% of the total drug amount; the drug content in the rice husks of the synergistic quinestrol bait accounts for 83.3% of the total drug amount, and the drug content in the brown rice accounts for 16.7% of the total drug amount. The sterile agent synergistic nano microemulsion can achieve rapid and effective dissolution of quinestrol and significantly increase the content of quinestrol in brown rice to 2 times that of the control group.

[0073] The description of the above embodiments is only used to help understand the technical solution and its core idea of the present invention. It should be pointed out that for those skilled in the art of this technology, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A nano microemulsion for enhancing the efficacy of rodent sterilants, characterized in that, It includes span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, polyoxyethylene hydrogenated castor oil and water, and the volume ratio of span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, diethylene glycol, polyoxyethylene hydrogenated castor oil and water is 10:20:40:30:20:1000, 10:40:30:50:20:1000, 20:50:50:50:50:2000 or 10:20:20:50:20:1000.

2. A preparation method of the nano microemulsion for enhancing the efficacy of rodent sterilant as claimed in claim 1, comprising the following steps: (1) Mix the formula amounts of span80, sodium dodecyl diphenyl ether disulfonate, isomeric alcohol polyoxyethylene ether, polyoxyethylene hydrogenated castor oil and diethylene glycol solution; (2) Add water to the above solution for dilution, with the water temperature at 60 - 80°C; stir until completely dissolved to obtain the nano microemulsion for enhancing the efficacy of rodent sterilant.

3. A rodent bait material, characterized in that: It contains the nano microemulsion for enhancing the efficacy of rodent sterilant as claimed in claim 1 as an active ingredient.

4. Application of the nano microemulsion for enhancing the efficacy of rodent sterilant as claimed in claim 1 in preparing rodent bait materials.

5. The application according to claim 4, characterized in that, It includes the following steps: (1) Add a rodent sterilant to the nano microemulsion for enhancing the efficacy of rodent sterilant and stir until uniform to form a sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilant; (2) Add bait materials to the sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilant in step (1), stir evenly, and dry or bake to obtain the rodent bait material containing the nano microemulsion for enhancing the efficacy of rodent sterilant.

6. The application according to claim 5, characterized in that, The addition amount of the rodent sterilant is to add 5 - 20 g of the rodent sterilant per 1000 mL of the nano microemulsion for enhancing the efficacy of rodent sterilant; the addition amount of the bait materials is to add 40 - 500 kg of bait materials per 1000 mL of the sterilant solution containing the nano microemulsion for enhancing the efficacy of rodent sterilant.

7. The application according to claim 4, 5 or 6, characterized in that, The rodent sterilant is a fat-soluble sterilant or a water-soluble sterilant.

8. The application according to claim 4, 5 or 6, characterized in that, The rodent sterilant is quinestrol, chlormadinone acetate or cabergoline.

9. The application according to claim 4, 5 or 6, characterized in that The bait materials are selected from one or more of paddy rice and wheat.

10. Application of the nano microemulsion for enhancing the efficacy of rodent sterilant as claimed in claim 1 or the rodent bait material as claimed in claim 3 in inhibiting rodent reproduction.

Images