Application of antibacterial compound in inhibiting Vibrio, antibacterial and cleaning composition

Abstract

The present invention provides an application of an antibacterial compound in inhibiting Vibrio, an antibacterial and washing composition, and relates to the technical field of antibacterial compounds. The compound used to inhibit Vibrio of the present invention is of natural origin, non-antibiotic, and has a very effective inhibitory effect on Vibrio. The Vibrio-inhibiting composition provided by the present invention contains 0.005% to 0.1% of the above-mentioned antibacterial compound and 0.005% to 0.5% of citrate. The antibacterial effect of the antibacterial compound is further enhanced by citrate, filling the technical gap in fruit and vegetable dishwashing detergents and hand cleansers in inhibiting the growth of Vibrio.

Description

Technical Field

[0001] The invention relates to the technical field of antibacterial compounds, in particular to an application of an antibacterial compound in inhibiting Vibrio, and an antibacterial and cleaning composition. Background Art

[0002] Vibrio bacteria are widely found in aquatic and marine environments, and can cause seafood and fish contamination and human infectious diseases. Internationally recognized pathogenic Vibrio include 11 human pathogens such as Vibrio cholerae, Vibrio metschnikovii, Vibrio parahaemolyticus, and Vibrio vulnificus. Due to the problem of excessive use of antibiotics in recent years, the presence of a variety of microbial non-degradable antibiotics has been found in aquatic environments, which can lead to the development of drug resistance in human and aquatic pathogens.

[0003] The emergence of antibiotic-resistant pathogenic Vibrio will seriously threaten human health. Careless use of tableware contaminated by these bacteria or consumption of contaminated food such as fish, seafood, and fruits and vegetables will lead to severe abdominal pain, diarrhea, hemolysis, and even death. However, the fruit and vegetable tableware washing industry and the food industry are currently focusing on Escherichia coli, Staphylococcus aureus, and Salmonella in terms of sterilization and antibacterial inhibition. There is still a lack of sufficient attention and effective preventive measures for pathogenic Vibrio infections and the health problems they cause.

[0004] In recent years, reports of Vibrio infections caused by eating food contaminated by pathogenic Vibrio and causing serious gastrointestinal diseases and hemolysis have increased year by year. Therefore, it is urgent to develop a new antibacterial agent that is superior to broad-spectrum antibiotics and can be safely and effectively used in the fruit and vegetable tableware washing industry and the food seasoning industry to inhibit the growth and reproduction of pathogenic Vibrio and thus reduce the risk of oral infection by Vibrio. In addition, the use of non-antibiotic, biodegradable compounds / compounds of natural origin is an inevitable trend in the development of antibacterial and antibacterial products, and has broad market application prospects.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The first purpose of the present invention is to provide an application of an antibacterial compound in inhibiting Vibrio. The antibacterial compound is of natural origin, is non-antibiotic, and has an effective inhibitory effect on Vibrio.

[0007] The second object of the present invention is to provide a composition for inhibiting Vibrio, wherein the antibacterial effect of the antibacterial compound is further enhanced by citrate.

[0008] The third object of the present invention is to provide a cleaning composition, which alleviates the problem of the prior art lacking a cleaning product having an inhibitory effect on Vibrio.

[0009] The fourth object of the present invention is to provide the use of the above-mentioned antibacterial compound, the above-mentioned Vibrio-inhibiting composition or the above-mentioned cleaning composition.

[0010] In order to solve the above technical problems, the present invention particularly adopts the following technical solutions:

[0011] According to one aspect of the present invention, there is provided the use of an antibacterial compound of formula (I) in inhibiting Vibrio;

[0012]

[0013] Among them, R 1 OH or OCH 3 , R 2 is H or OH, R 3 is H or OH.

[0014] Preferably, the Vibrio includes at least one of Vibrio vulnificus and Vibrio alginolyticus;

[0015] Preferably, in formula (I), R 1 For OH, R 2 For H, R 3 is H, and the antibacterial compound of formula (Ⅰ) is cinnamic acid;

[0016] Preferably, in the application, the working concentration of the antibacterial compound of formula (I) is 0.005% to 0.1%, preferably 0.01% to 0.1%, and more preferably 0.05%.

[0017] Preferably, the antibacterial compound of formula (I) and citrate are used in combination in the application;

[0018] Preferably, the citrate comprises one or more of sodium citrate, sodium dihydrogen citrate, potassium citrate and ammonium citrate;

[0019] Preferably, in the application, the working concentration of the citrate is 0.005% to 0.5%, preferably 0.01% to 0.5%, and more preferably 0.1%.

[0020] According to one aspect of the present invention, there is also provided a composition for inhibiting Vibrio, comprising an antibacterial compound of formula (I) and a citrate;

[0021]

[0022] Among them, R 1 OH or OCH 3 , R2 is H or OH, R 3 is H or OH;

[0023] The working concentration of the antibacterial compound of formula (I) in the composition is 0.005% to 0.1%, and the working concentration of the citrate is 0.005% to 0.5%.

[0024] According to one aspect of the present invention, there is also provided a cleaning composition comprising (a) or (b), and a cleaning component;

[0025] (a) Antibacterial compounds of formula (I):

[0026]

[0027] Among them, R 1 OH or OCH 3 , R 2 is H or OH, R 3 is H or OH.

[0028] (b) The above-mentioned Vibrio-inhibiting composition.

[0029] According to one aspect of the present invention, there is also provided the above-mentioned composition for inhibiting Vibrio, or the use of the above-mentioned cleaning composition in at least one of the following:

[0030] (i) Preparation of antibacterial agents; (ii) Preparation of cleaning agents; (iii) Preparation of antibacterial drugs; (iv) Inhibition of Vibrio for non-diagnostic and non-therapeutic purposes; (v) At least one of fruit and vegetable cleaning, tableware cleaning and hand cleaning.

[0031] According to one aspect of the present invention, there is also provided an antibacterial product, which includes an antibacterial agent, a cleaning agent or an antibacterial drug. The antibacterial product includes the above-mentioned Vibrio inhibiting composition or the above-mentioned cleaning composition.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The antibacterial compound shown in formula (I) provided by the present invention is derived from plant extracts and is a non-antibiotic antibacterial compound. Experiments have confirmed that it can effectively inhibit the growth of Vibrio at a relatively low concentration. The experiment also found that after the combination of it and citrate, the effect of inhibiting Vibrio will be further enhanced. Based on this discovery, the antibacterial composition and washing composition provided by the present invention have the effect of inhibiting Vibrio. The antibacterial compound, the antibacterial composition and the washing composition are applied to antibacterial agents and cleaning agents, filling the technical gap in fruit and vegetable dishwashing detergents and hand cleansers in inhibiting the growth of Vibrio. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1 The inhibitory effect of the detergent composition with or without cinnamon extract added in Effect Example 1 of the present invention on the growth of Vibrio vulnificus;

[0036] Figure 2 The inhibitory effect of the detergent composition with or without cinnamon extract added in Effect Example 1 of the present invention on the growth of Vibrio alginolyticus;

[0037] Figure 3 The inhibitory effect of adding / not adding cinnamon extract-citrate complex detergent on the growth of Vibrio vulnificus in Effect Example 2 of the present invention;

[0038] Figure 4 The inhibitory effect of adding / not adding cinnamon extract-citrate complex detergent on the growth of Vibrio alginolyticus in Effect Example 2 of the present invention;

[0039] Figure 5 The figures are the inhibitory effects of cinnamic acid and rosemary extract on the growth of Vibrio alginolyticus in Effect Example 3 of the present invention. DETAILED DESCRIPTION

[0040] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0042] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0043] It should be further understood that the term "and / or" used in the present description and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0044] All percentages, parts and ratios are calculated based on the total weight of the compositions of the present invention, unless otherwise specified.

[0045] All weights as they pertain to listed ingredients are based on the active level and, therefore, do not include solvents or by-products that may be included in commercially available materials, unless otherwise specified. "%" herein refers to percent by weight.

[0046] According to one aspect of the present invention, there is provided a use of an antibacterial compound in inhibiting Vibrio, wherein the antibacterial compound is as shown in formula (I);

[0047]

[0048] Among them, R 1 OH or OCH 3 , R 2 is H or OH, R 3 is H or OH.

[0049] In the application provided by the present invention, the antibacterial compound is derived from a plant extract and is a non-antibiotic antibacterial compound. It should be noted that in the practical application of the present invention, the source of the compound represented by formula (I) is not limited, and it can be a compound extracted from a plant, or a compound obtained by chemical synthesis to meet the requirements of formula (I), or a compound extracted from a plant and further processed artificially.

[0050] In an optional embodiment, in formula (I), R 1 For OH, R 2 For H, R 3 is H, and the antibacterial compound of formula (I) is cinnamic acid.

[0051] In an optional embodiment, the vibrio includes at least one of Vibrio vulnificus and Vibrio alginolyticus. Experimental verification shows that the above formula (I) can effectively inhibit the vibrio including Vibrio vulnificus and Vibrio alginolyticus.

[0052] In an optional embodiment, in the application, the working concentration of the antibacterial compound of formula (I) is 0.005% to 0.1%, for example, it can be but not limited to 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or 0.1%, or a range between any two of the above points, preferably 0.01% to 0.1%, and more preferably 0.05%.

[0053] In an optional embodiment, the antibacterial compound of formula (I) and citrate are used in combination in the application. Experiments have found that citrate has a synergistic effect on the inhibition of Vibrio by the antibacterial compound of formula (I). The combined use of the antibacterial compound of formula (I) and citrate has a better inhibitory effect on Vibrio than the antibacterial compound of formula (I) alone.

[0054] The citrate used in combination with the antibacterial compound of formula (I) can be selected from conventional citrates in the art, including but not limited to one or more of sodium citrate, sodium dihydrogen citrate, potassium citrate and ammonium citrate. In an optional embodiment, in the application, the working concentration of the citrate is 0.005% to 0.5%, for example, it can be but not limited to 0.005%, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4% or 0.5%, or the range between any two of the above points, preferably 0.01% to 0.5%, and more preferably 0.1%.

[0055] Based on the concept of using the antibacterial compound of formula (I) and citrate in combination to inhibit Vibrio, the present invention also provides a composition for inhibiting Vibrio, which comprises the antibacterial compound of formula (I) and citrate. In an optional embodiment, in formula (I), R 1 For OH, R 2 For H, R 3 is H, and the antibacterial compound of formula (I) is cinnamic acid. The citrate can be selected from conventional citrates in the art, including but not limited to one or more of sodium citrate, sodium dihydrogen citrate, potassium citrate and ammonium citrate. In an optional embodiment, the citrate includes sodium citrate.

[0056] In the antibacterial composition provided by the present invention, the working concentration of the antibacterial compound of formula (I) is 0.005% to 0.1%, for example, it can be but not limited to 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or 0.1%, or the range between any two of the above points. The working concentration of citrate is 0.005% to 0.5%, for example, it can be but not limited to 0.005%, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4% or 0.5% or the range between any two of the above points.

[0057] In an optional embodiment, the working concentration of the antibacterial compound of formula (I) in the Vibrio inhibiting composition is 0.01% to 0.1%, and the working concentration of citrate is 0.01% to 0.5%.

[0058] In an optional embodiment, the working concentration of the antibacterial compound of formula (I) in the Vibrio inhibiting composition is 0.05%, and the working concentration of citrate is 0.1%.

[0059] According to one aspect of the present invention, there is provided a cleaning composition comprising (a) or (b), and a cleaning component;

[0060] (a) the antibacterial compound of the above formula (I):

[0061] (b) The Vibrio-inhibiting composition according to the aforementioned embodiment.

[0062] In an optional embodiment, the washing components include conventional cleaning components in the art, which are not limited in the present invention, and include but are not limited to at least one of surfactants, solvents, enzymes, polymers and auxiliaries; 0% in the following embodiment means that the component is not contained.

[0063] In an optional embodiment, the content of the polymer is 0% to 5%, for example, it can be but not limited to 0.1%, 0.5%, 1%, 2%, 3%, 4% or 5%, or the range between any two of the above points. The polymer is an optional polymer acceptable in the field of washing, for example, but not limited to a polymer used for rheology modifier, anti-redeposition agent, fluorescent whitening agent or phosphorus substitute. Specific examples of polymers include but are not limited to hydroxyethyl cellulose, carboxymethyl cellulose, polyacrylate, polyurethane, polyacrylate, methyl cellulose ether, polycarboxylate, sodium tripolyphosphate or maleic acid methyl methacrylate copolymer, etc., and the present invention does not limit this.

[0064] In an optional embodiment, the surfactant includes one or a mixture of cationic surfactants, nonionic surfactants and anionic surfactants, preferably including cationic surfactants and / or nonionic surfactants. In an optional embodiment, the anionic surfactant includes but is not limited to one or a combination of carboxylates, sulfonates, sulfates and phosphates; in an optional embodiment, the cationic surfactant includes one or a combination of quaternary ammonium surfactants, heterocyclic surfactants, and polymeric cationic surfactants; in an optional embodiment, the nonionic surfactant includes but is not limited to one or a combination of fatty alcohol alkoxylates, fatty acid alkoxylates, fatty acid methyl ester ethoxylates, alkyl polysaccharides, fatty acid alkyl alcohol amides and ethoxylated anhydrous sorbitan esters.

[0065] In an optional embodiment, the content of surfactant in the detergent composition is 0% to 15%, for example, it can be but not limited to 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%, or a range between any two of the above points.

[0066] In an optional embodiment, when the detergent composition contains a cationic surfactant, the concentration of the cationic surfactant is no more than 10%, for example, it can be but not limited to 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, or a range between any two of the above points.

[0067] In an optional embodiment, the solvent includes but is not limited to monopropylene glycol and / or methylpropylene glycol;

[0068] In an optional embodiment, the solvent content is 0% to 15%, for example, it can be but not limited to 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%, or a range between any two of the above points.

[0069] In an optional embodiment, the enzyme includes, but is not limited to, one or a combination of proteases, amylases, cellulases, lipases, mannanases, pectinases, pectin lyases and xanthanases.

[0070] In an optional embodiment, the content of the enzyme is 0% to 0.5%, for example, it can be but not limited to 0.1%, 0.2%, 0.3%, 0.4% or 0.5%, or a range between any two of the above points.

[0071] In an optional embodiment, the auxiliary agent includes one or a combination of a co-solvent, a pH stabilizer and a flavor.

[0072] In an optional embodiment, the washing component is as shown in Table 1, including a surfactant, a solvent and other components, wherein 0% means that the corresponding component is not contained, but it should be noted that the washing component described in Table 1 includes at least one surfactant, at least one solvent and at least one other component. The substrate can be a conventional substrate for washing components in the art, such as water.

[0073] Table 1

[0074]

[0075]

[0076] In an optional embodiment, the detergent components include 8% fatty alcohol polyoxyethylene ether, 2% sodium alkylbenzene sulfonate, 2% alkyl glucoside, 2% propylene glycol, 0.01% sodium chloride and 0.1% fragrance.

[0077] In an alternative embodiment, the cleaning composition is water-based.

[0078] In an optional embodiment, the above-mentioned cleaning composition is prepared as follows: the antibacterial compound of formula (I), or the antibacterial compound of formula (I) and citrate are respectively dissolved or dispersed in an appropriate amount of water to obtain a mother liquor with a higher concentration; the above-mentioned surfactant is dissolved in water, and then the remaining components are added, and then the above-mentioned antibacterial compound of formula (I), or the antibacterial compound of formula (I) and citrate mother liquor are added, and then an appropriate amount of water is added to disperse evenly.

[0079] In an optional embodiment, the detergent composition includes 0.005% to 0.1% of the antibacterial compound of formula (I), for example, it can be but not limited to 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or 0.1%, or a range between any two of the above points; or, it includes 0.005% to 0.1% of the antibacterial compound of formula (I), for example, it can be but not limited to 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09% or 0.1%, or a range between any two of the above, and 0.005% to 0.5% citrate, for example, but not limited to 0.005%, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4% or 0.5% or a range between any two of the above.

[0080] In an optional embodiment, the cleaning composition comprises 0.01% to 0.1% of the antibacterial compound of formula (I) above, or comprises 0.01% to 0.1% of the antibacterial compound of formula (I) above and 0.01% to 0.5% of citrate.

[0081] In an optional embodiment, the cleaning composition includes 0.05% of the antibacterial compound of formula (I), or includes 0.01% to 0.1% of the antibacterial compound of formula (I) and 0.1% of citrate.

[0082] In an optional embodiment, the antibacterial compound of formula (I) is cinnamic acid.

[0083] In an optional embodiment, the citrate is sodium citrate.

[0084] According to another aspect of the present invention, there is also provided the above-mentioned composition for inhibiting Vibrio, or the use of the above-mentioned cleaning composition in at least one of the following:

[0085] (i) Preparation of antibacterial agents; (ii) Preparation of cleaning agents; (iii) Preparation of antibacterial drugs; (iv) Inhibition of Vibrio for non-diagnostic and non-therapeutic purposes; (v) At least one of fruit and vegetable cleaning, tableware cleaning and hand cleaning.

[0086] According to another aspect of the present invention, a kind of antibacterial product is also provided, and the antibacterial product includes an antibacterial agent, a cleaning agent or an antibacterial drug, which includes the above-mentioned composition for inhibiting Vibrio, or the above-mentioned washing composition. The antibacterial product, according to its specific type, also includes optional adjuvants acceptable to the corresponding field, for example, when the antibacterial product is a cleaning agent or an antibacterial agent, it also optionally includes an adjuvant acceptable to the field of daily chemicals. In an optional embodiment, the cleaning agent is a fruit and vegetable cleaning agent or a hand cleaning agent. When the antibacterial product is an antibacterial drug, it also optionally includes optional adjuvants acceptable to the pharmaceutical field, including but not limited to one or more of excipients, solvents, diluents, pH adjusting agents, preservatives, humectants and cosolvents.

[0087] The present invention is further described below by means of specific examples. However, it should be understood that these examples are only used for more detailed description and should not be construed as limiting the present invention in any form.

[0088] The pathogenic Vibrio described in the following implementations are Vibrio alginolyticus and Vibrio vulnificus.

[0089] Example 1

[0090] This embodiment provides a cleaning composition, including a model cleaning composition and cinnamic acid (Cinnamicacid, CA). The composition of the model cleaning composition is shown in Table 2, and the content of cinnamic acid is 0.05%.

[0091] Table 2

[0092] Material Name content / % Fatty alcohol polyoxyethylene ether 8 Sodium alkylbenzene sulfonate 2 Alkyl glucoside 2 Propylene glycol 2 Sodium chloride 0.01 essence 0.1 Matrix Replenish to 100

[0093] Example 2

[0094] This embodiment provides a cleaning composition, including a model cleaning composition, cinnamic acid (CA) and sodium citrate. The composition of the model cleaning composition is shown in Table 2, the content of cinnamic acid is 0.05%, and the content of citrate is 0.1%.

[0095] Example 3

[0096] This embodiment provides a cleaning composition, including a model cleaning composition, cinnamic acid (CA) and sodium citrate. The composition of the model cleaning composition is shown in Table 2, the content of cinnamic acid is 0.005%, and the content of citrate is 0.5%.

[0097] Example 4

[0098] This embodiment provides a cleaning composition, including a model cleaning composition, cinnamic acid (CA) and sodium citrate. The composition of the model cleaning composition is shown in Table 2, the content of cinnamic acid is 0.1%, and the content of citrate is 0.005%.

[0099] Example 5

[0100] This embodiment provides a cleaning composition, including a model cleaning composition and rosmarinic acid (RA), the composition of the model cleaning composition is shown in Table 2, and the content of rosmarinic acid is 0.05%. Rosmarinic acid is shown in formula (II):

[0101]

[0102] Among them, R 1 C 9 O 5 H 8 , R 2 For OH, R 3 For OH.

[0103] Comparative Example 1:

[0104] This comparative example provides a washing composition, which only includes a model washing composition. The composition of the model washing composition is shown in Table 2.

[0105] Comparative Example 2

[0106] This comparative example provides a washing composition, including a model washing composition and sodium citrate. The composition of the model washing composition is shown in Table 2, and the content of the citrate is 0.1%.

[0107] Effect example

[0108] The method for evaluating the effect of inhibiting Vibrio growth in the following effect examples is as follows:

[0109] 1. Inoculate Vibrio vulnificus and Vibrio alginolyticus into Marine broth medium (MB medium) respectively, culture at 37°C until OD600 = 0.6-0.8, add glycerol at a volume ratio of 1:1 (final glycerol concentration 50%), and dispense 10 μL into cryopreservation tubes. After aliquoting, quickly freeze in liquid nitrogen to obtain Vibrio cryopreservation solution that can be stored and used for a long time.

[0110] 2. Experiment on evaluating the effect of inhibiting Vibrio growth: Take 10 μL of the above frozen solution, re-dissolve it in a 30°C water bath, add it into 5 ml of MB liquid culture medium containing 0.2% to 0.5% DMSO, and culture it at 30°C until OD600 = 0.6 to 0.8.

[0111] 3. Inoculate 10 μL of the culture medium in 2 into 5 ml of the test sample and shake for 2 h at 30°C.

[0112] 4. Inoculate 2 μL of the treated solution in 3 into 200 μL MB medium and culture at 30°C. Measure the OD600 value of the bacterial solution every hour. Repeat each experiment 3 times.

[0113] The antibacterial rate of the test object was calculated according to the OD600 value when the microorganism reached its growth stationary phase. The calculation formula is as follows:

[0114]

[0115] Exp means test group, and Con means control group.

[0116] The composition of the MB medium is: 3.0 g / L meat extract, 5.0 g / L meat peptone, 10.0 g / L sodium chloride, and phosphate buffer adjusted to pH 7.0.

[0117] Effect Example 1

[0118] Inhibitory effect of detergents with / without cinnamon extract on the growth of Vibrio vulnificus and Vibrio alginolyticus:

[0119] The test samples were prepared as follows:

[0120] Example 1 The detergent composition test sample was prepared as follows: cinnamic acid was first prepared into a stock solution with a concentration of 0.5%, and 0.5 ml of cinnamic acid stock and the remaining components of the detergent composition according to the formula amounts recorded in Example 1 were added to 5 ml of MB culture medium.

[0121] Comparative Example 1 The detergent composition test sample was prepared as follows: each detergent composition component in Comparative Example 1 was added to 5 ml of MB medium according to the formula amount recorded in Comparative Example 1.

[0122] A blank control was set up and the experimental groups were as follows:

[0123] Table 3 Experimental groups

[0124] Group MB medium Mode detergent 0.05% CA Blank control group + - - Comparative Example 1 + + - Example 1 + + +

[0125] Experimental results:

[0126] (1) Vibrio vulnificus:

[0127] The results show that the detergent composition (Example 1) with 0.05% cinnamic acid (CA) added has a significant inhibitory effect on the growth of Vibrio vulnificus. After being treated with the detergent composition of Example 1, the growth of Vibrio vulnificus is still stably inhibited even after a culture period of up to 10 hours. When the culture time is 10 hours, the inhibition rates of different test samples are shown in Table 4:

[0128] Table 4 Inhibition rate of Vibrio vulnificus after 10h of culture time

[0129] Antibacterial rate (%) Blank control group —— Comparative Example 1 —— Example 1 77.7

[0130] The results show that the treatment with comparative example 1 (model detergent composition) has no significant inhibitory effect on the growth of Vibrio vulnificus. After the treatment with example 1, the inhibition rate of the growth of Vibrio vulnificus is still as high as 77.7% at a culture time of 10 hours.

[0131] (2) Vibrio alginolyticus:

[0132] The results show that the detergent composition (Example 1) with 0.05% cinnamic acid (CA) added has a significant inhibitory effect on the growth of Vibrio alginolyticus. After being treated with the detergent composition of Example 1, the growth of Vibrio alginolyticus was stably inhibited for a culture time of 0 to 10 hours. When the culture time was 10 hours, the inhibition rates of different test samples are shown in Table 5:

[0133] Table 5 Inhibition rate of Vibrio alginolyticus after 10 h of culture

[0134] Antibacterial rate (%) Blank control group —— Comparative Example 1 —— Example 1 88.1

[0135] The treatment with comparative example 1 (model detergent) had no inhibitory effect on the growth of Vibrio alginolyticus. After the treatment with example 1, even after 10 hours of cultivation, the inhibition rate on the growth of Vibrio alginolyticus was still as high as 88.1%.

[0136] Effect Example 2

[0137] The test samples for the inhibitory effect of cinnamon extract-citrate complex on the growth of Vibrio vulnificus and Vibrio alginolyticus with / without addition were prepared as follows:

[0138] The preparation method of Comparative Example 1 is the same as that of Effect Example 1.

[0139] Example 2 The detergent composition test sample was prepared as follows: cinnamic acid and sodium citrate were prepared into stocks with concentrations of 0.5% and 1%, respectively, and 0.5 ml of the stock and the remaining components of the detergent composition were added to 5 ml of MB culture medium according to the formula amounts recorded in Example 2.

[0140] Comparative Example 2 The detergent composition test sample was prepared as follows: sodium citrate was prepared into a stock with a concentration of 1%, and 0.5 ml of the stock and the remaining components of the detergent composition according to the formula amounts recorded in Comparative Example 2 were added to 5 ml of MB culture medium.

[0141] A blank control was set up and the experimental groups were as follows:

[0142] Table 6 Effect Example 2 Experimental Grouping

[0143]

[0144] Experimental results:

[0145] (1) Vibrio vulnificus: The inhibitory effects and inhibition rates of different test samples on the growth of Vibrio vulnificus are as follows: Figure 3 And as shown in Table 7.

[0146] The results show that Comparative Example 2 (model detergent with 0.1% SC added) has a certain inhibitory effect on Vibrio vulnificus when cultured for 0 to 6 hours, but after 7 hours, there is no significant inhibitory effect on the growth of Vibrio vulnificus, that is, 0.1% SC has no significant inhibitory effect on Vibrio vulnificus. After being treated with Example 2 (model detergent composition with 0.05% CA-0.1% SC complex added), the growth of Vibrio vulnificus has been stably inhibited for 0 to 10 hours of culture time. When the culture time is 10 hours, the inhibition rates of different test samples are shown in Table 7:

[0147] Table 7 Inhibition rate of different test samples against Vibrio vulnificus after 10 h of incubation

[0148] Antibacterial rate (%) Blank control group —— Comparative Example 1 —— Comparative Example 2 —— Example 2 82.5

[0149] After treatment with Example 2 (a model detergent with 0.05% CA-0.1% SC complex added), after 10 hours of cultivation, the inhibition rate of Vibrio vulnificus growth was 82.5%, which was slightly higher than the inhibition rate in Example 1 (77.7%), indicating that SC has a certain synergistic effect on CA in the antibacterial effect on Vibrio vulnificus.

[0150] (2) Vibrio alginolyticus: The inhibitory effects and inhibition rates of the same test samples on the growth of Vibrio alginolyticus are as follows: Figure 4 As shown in Table 8:

[0151] The results show that the growth inhibition effect of Comparative Example 2 (model detergent with 0.1% SC added) on Vibrio alginolyticus is not significant, that is, 0.1% SC has no significant antibacterial effect on Vibrio alginolyticus. After treatment with Example 2 (model detergent with 0.05% CA-0.1% SC complex added), the growth of Vibrio alginolyticus has been stably inhibited for 0 to 10 hours of incubation time. When the incubation time is 10 hours, the antibacterial rates of different test samples are shown in Table 8:

[0152] Table 8 Inhibition rate of different test samples against Vibrio alginolyticus after 10 h of incubation

[0153] Group Antibacterial rate (%) Blank control group —— Comparative Example 1 —— Comparative Example 2 —— Example 2 88.9

[0154] After treatment in Example 2, the inhibition rate of Vibrio alginolyticus growth was 88.9% after 10 hours of culture, which was not significantly different from the inhibition rate (88.1%) in Example 1. This indicates that unlike the synergistic effect on the antibacterial effect of Vibrio vulnificus, the antibacterial effect of SC on CA did not show a synergistic effect on Vibrio alginolyticus.

[0155] Effect Example 3

[0156] Comparison of the inhibitory effects of cinnamic acid (CA) and rosmarinic acid (RA) on the growth of Vibrio alginolyticus:

[0157] The test samples were prepared as follows:

[0158] The preparation methods of Comparative Example 1 and Example 1 are the same as those of Effect Examples 1 and 2.

[0159] Example 5 The detergent composition test sample was prepared as follows: rosmarinic acid was prepared into a stock with a concentration of 0.5%, and 0.5 ml of the stock and the remaining components of the detergent composition according to the formula amounts recorded in Example 5 were added to 5 ml of MB culture medium, and the final concentration of rosmarinic acid was 0.05%.

[0160] A blank control was set up and the experimental groups were as follows:

[0161] Table 9 Effect Example 3 Experimental Grouping

[0162] Group MB medium Model washing composition 0.05% CA 0.05% RA Blank control + - - - Comparative Example 1 + + - - Example 5 + + - + Example 1 + + + -

[0163] Experimental results: The inhibitory effects and inhibition rates of different test samples on the growth of Vibrio alginolyticus are as follows: Figure 5 As shown in Table 10:

[0164] The results show that Example 5 (a model detergent composition with 0.05% RA added) and Example 1 (a model detergent composition with 0.05% CA added) both exhibited significant inhibitory effects on the growth of Vibrio alginolyticus, and the inhibitory effect of Example 1 was superior to that of Example 5, and when the culture time was greater than 7 hours, the inhibitory effect of Example 5 gradually weakened, but the inhibitory effect of Example 1 was still relatively stable. After being treated with different test samples and cultured for 10 hours, the inhibitory rate of Vibrio alginolyticus growth is shown in Table 10.

[0165] Table 10 Inhibition rate of different test samples against Vibrio alginolyticus after 10 h of incubation

[0166] Antibacterial rate (%) Blank control —— Comparative Example 1 —— Example 5 67.8 Example 1 87.8

[0167] After being treated in Example 1, the inhibition rate of Vibrio alginolyticus growth was 87.8% after 10 hours of culture, which was significantly higher than 67.8% in Comparative Example 1. This indicates that the cinnamic acid extract and the composition thereof described in the present invention are significantly better than rosemary extract in inhibiting the growth of pathogenic Vibrio.

[0168] Effect Example 4

[0169] The test sample preparation is shown in Example 1. The inhibition rates of different test samples on the growth of Vibrio alginolyticus / Vibrio vulnificus are shown in Table 11:

[0170] Antibacterial rate (%) Example 3 14.5 / 15.5 Example 4 90.5 / 90.2

[0171] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Application of cinnamic acid in inhibiting Vibrio, wherein the Vibrio is Vibrio vulnificus or Vibrio alginolyticus; in the application, the working concentration of cinnamic acid is 0.005% to 0.1%.

2. The use according to claim 1, It is characterized in that The working concentration of the cinnamic acid is 0.05% to 0.1%.

3. The use according to any one of claims 1 to 2, It is characterized in that In the application, cinnamic acid and citrate are used in combination, and the working concentration of the citrate is 0.005% to 0.5%; the citrate is sodium citrate, sodium dihydrogen citrate, potassium citrate or ammonium citrate.

4. The use according to claim 3, It is characterized in that The working concentration of the citrate is 0.1% to 0.5%.

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