A plant activator and its application

Abstract

The present invention discloses a plant activator and its application, belonging to the technical field of plant growth regulation. The active ingredients in the plant activator include hinokitiol and rape pollen extract. The present invention first combines hinokitiol and rape pollen extract as the active ingredients of the plant activator. The two cooperate with each other and jointly play a role in promoting the self-growth of crops, and can significantly improve the yield and quality of crops such as tomatoes. At the same time, it can reduce the use of chemical drugs, which is of great significance for improving the quality and efficiency of crops and the sustainable development of agriculture.

Description

Technical Field

[0001] The present invention relates to the technical field of plant growth regulation, and in particular, to a plant activator and its application. Background Art

[0002] Sabinol, also known as hinokitiol, is a monoterpenoid natural compound with a tropolone skeleton extracted from the trunk of Chamaecyparis obtusa var. formosana. It belongs to the tropolone family of compounds, has relatively wide biological activities and strong bactericidal ability, is a plant component with high safety, and is often used as an active ingredient in antibacterial agents and insect repellents.

[0003] In addition to containing metabolic product components such as proteins, active polypeptides, amino acids, vitamins, flavonoids, phytosterols, fatty acids and their derivatives, polyphenols (flavonoids), nitrogen-containing compounds, etc., rape pollen also contains active components such as polysaccharides, and has the functions of enhancing human immune function, antioxidant, anti-aging, improving digestive tract function, anti-fatigue, regulating blood lipids, inhibiting prostate hyperplasia, etc., and is often used as an efficacy component of health products.

[0004] At present, with the continuous development of chemical engineering technology and biotechnology, there are more and more research reports on plant activators. Plant activators can regulate and promote the self-growth ability of plants, and play an increasingly important role in improving the yield and quality of crops. Although there are a variety of existing plant activators, there is no report in the prior art on the application of combining sabinol and rape pollen extract in the preparation of plant activators. Summary of the Invention

[0005] The purpose of the present invention is to provide a plant activator and its application, which for the first time combines sabinol and rape pollen extract as the active ingredients of the plant activator, can promote the self-growth of crops, and significantly improve the yield and quality of crops such as tomatoes.

[0006] In a first aspect, an embodiment of the present application provides a plant activator, and the active ingredients in the plant activator include sabinol and rape pollen extract.

[0007] Furthermore, the weight ratio of sabinol to the rape pollen extract is (4~7):1.

[0008] Furthermore, the rape pollen extract is an alcohol extract of rape pollen.

[0009] Furthermore, the preparation method of the alcohol extract of rape pollen includes the following steps:

[0010] Mix the rapeseed pollen after wall-breaking treatment and an ethanol aqueous solution with a mass fraction of 60%~70% according to a weight ratio of 1:(8~12) to obtain a mixed solution;

[0011] Place the mixed solution in a water bath at a temperature of 55 - 65 °C for ultrasonic extraction for 40 - 60 minutes, and then filter and evaporate to concentrate to obtain a crude alcohol extract of rape pollen;

[0012] Pass the crude alcohol extract of rape pollen through a macroporous resin column. First, elute with an aqueous ethanol solution with a mass fraction of 2% - 3% at a flow rate of 2 - 3 times the column volume per hour, with an elution volume of 3 times the column volume, and discard the first eluate; then elute with an aqueous ethanol solution with a mass fraction of 80% - 85% at a flow rate of 2 - 3 times the column volume per hour, with an elution volume of 5 - 8 times the column volume, and collect the second eluate;

[0013] Concentrate and dry the second eluate to obtain an alcohol extract of rape pollen.

[0014] Further, the working parameters of the ultrasonic extraction include: an ultrasonic frequency of 30 - 40 kHz and a power of 700 - 850 W.

[0015] Further, the plant activator further includes an alcohol solvent.

[0016] Further, in terms of weight percentage, the concentration of the active ingredient in the plant activator is 5 - 8 g / L.

[0017] In a second aspect, the embodiments of the present application provide the application of the plant activator described in the first aspect in improving the quality and yield of crops.

[0018] In a third aspect, the embodiments of the present application provide a method for improving the quality and yield of tomatoes, the method comprising the following steps:

[0019] Add the plant activator described in any item of the first aspect to the planting soil for mixing to obtain a planting soil containing the plant activator;

[0020] Transplant tomato seedlings into the planting soil containing the plant activator, and then carry out maintenance management.

[0021] Further, based on every 1 kg of dry soil, the addition amount of the plant activator is 10 - 20 g.

[0022] Compared with the prior art, the above - mentioned solutions provided by the embodiments of the present application have at least the following beneficial effects:

[0023] An embodiment of the present application provides a plant activator. The active ingredients of the plant activator include hinokitiol and rapeseed pollen extract. For the first time, hinokitiol and rapeseed pollen extract are used in combination as the active ingredients of the plant activator. The two work synergistically to promote the self-growth of crops, and can significantly improve the yield and quality of crops such as tomatoes. At the same time, the use of chemical drugs can be reduced, which is of great significance for improving the quality and efficiency of crops and the sustainable development of agriculture. Detailed implementation manners

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Those not specified in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments not specified by the manufacturer are all conventional products that can be obtained through commercial purchase.

[0025] It should be noted that, without conflict, the embodiments in the present application and the features in the embodiments may be combined with each other. The present invention will be described in detail below with reference to specific embodiments.

[0026] The general idea of the technical solution provided by the embodiment of the present invention is as follows:

[0027] In a first aspect, an embodiment of the present application provides a plant activator, and the active ingredients in the plant activator include hinokitiol and rapeseed pollen extract.

[0028] An embodiment of the present application provides a plant activator. The active ingredients of the plant activator include hinokitiol and rapeseed pollen extract. For the first time, hinokitiol and rapeseed pollen extract are used in combination as the active ingredients of the plant activator. The two work synergistically to promote the self-growth of crops, and can significantly improve the yield and quality of crops such as tomatoes. At the same time, the use of chemical drugs can be reduced, which is of great significance for improving the quality and efficiency of crops and the sustainable development of agriculture.

[0029] As an implementation manner of the embodiment of the present application, the weight ratio of the hinokitiol to the rapeseed pollen extract is (4-7):1.

[0030] The inventors of the present application further found through research that when the weight ratio of the hinokitiol to the rapeseed pollen extract is controlled to be (4-7):1, the synergistic effect of the two is stronger, which is more conducive to improving the yield and quality of crops such as tomatoes. More preferably, the weight ratio of the hinokitiol to the rapeseed pollen extract is 6:1.

[0031] As an implementation manner of the embodiment of the present application, the rapeseed pollen extract is an alcohol extract of rapeseed pollen.

[0032] In this application, the rape pollen extract is preferably the alcohol extract of rape pollen. In some specific embodiments, the preparation method of the alcohol extract of rape pollen includes the following steps:

[0033] Mix the wall-broken rape pollen and an ethanol aqueous solution with a mass fraction of 60% - 70% according to a weight ratio of 1:(8 - 12) to obtain a mixed solution;

[0034] Place the mixed solution in a water bath at a temperature of 55 - 65°C for ultrasonic extraction for 40 - 60 minutes, then filter and evaporate and concentrate to obtain a crude alcohol extract of rape pollen;

[0035] Pass the crude alcohol extract of rape pollen through a macroporous resin column of models such as AB - 8, D101, ADS - 7, etc. First, elute with an ethanol aqueous solution with a mass fraction of 2% - 3% at a flow rate of 2 - 3 times the column volume per hour, and the elution volume is 3 times the column volume. Discard the first eluate; then elute with an ethanol aqueous solution with a mass fraction of 80% - 85% at a flow rate of 2 - 3 times the column volume per hour, and the elution volume is 5 - 8 times the column volume. Collect the second eluate;

[0036] Concentrate and dry the second eluate to obtain the alcohol extract of rape pollen.

[0037] As an implementation manner of the embodiment of this application, the working parameters of the ultrasonic extraction include: the ultrasonic frequency is 30 - 40 kHz, and the power is 700 - 850 W.

[0038] In some specific embodiments, preferably, the working parameters of the ultrasonic extraction include: the ultrasonic frequency is 35 kHz, and the power is 800 W.

[0039] As an implementation manner of the embodiment of this application, the plant activator further includes an alcohol solvent.

[0040] In some specific embodiments, the alcohol solvent is preferably ethanol.

[0041] As an implementation manner of the embodiment of this application, calculated by weight percentage, the concentration of the active ingredient in the plant activator is 5 - 8 g / L.

[0042] The concentration of the active ingredient in the plant activator provided by this application is relatively low, and the effect is good. In some specific embodiments, the concentration of the active ingredient can be 5 g / L, 6 g / L, 7 g / L, 8 g / L, etc.

[0043] In the second aspect, the embodiment of this application provides the application of the plant activator described in the first aspect in improving the quality and yield of crops.

[0044] The embodiments of the present application provide a plant activator that can promote the self - growth of crops, significantly improve the yield and quality of crops such as tomatoes. At the same time, it can reduce the use of chemical drugs, which is of great significance for improving the quality and efficiency of crops and the sustainable development of agriculture.

[0045] In a third aspect, the embodiments of the present application provide a method for improving the quality and yield of tomatoes, the method comprising the following steps:

[0046] Add the plant activator according to any one of the first aspect into the planting soil and mix to obtain the planting soil containing the plant activator;

[0047] Transplant tomato seedlings into the planting soil containing the plant activator, and then carry out maintenance management.

[0048] The present application mixes the provided plant activator with the soil and then plants tomato seedlings, which can significantly improve the yield and quality of tomatoes.

[0049] In the present application, the planting of tomatoes and the subsequent maintenance management can be carried out according to the tomato planting techniques disclosed in the prior art. Specifically, for example:

[0050] Planting method: When planting, pre - drill holes and water, 1 - 1.5 kg per hole. When planting seedlings, the nursery is 0.5 - 1.0 cm lower than the kneading surface, the nursery is covered with 0.5 - 1.0 cm of fine soil, and use the index finger. Gently press both sides of the nursery with the middle finger to fix the seedlings, and then pour a small amount of water to make the nursery close to the surrounding soil. 2 - 3 days after planting, pour a watering for the seedlings to prevent the seedlings from losing water and wilting. It can also be planted first and then covered with seedlings. After planting, check the seedlings, replenish the seedlings in time to ensure the integrity of the seedlings, and transfer 1500 - fold liquid of cutting - edge with a sprayer.

[0051] The subsequent maintenance management includes:

[0052] (1) Temperature management: After planting, try to maintain a relatively high temperature to reduce seedlings. The suitable temperature is 28°C - 30°C during the day, 20°C - 18°C at night, and 20°C - 22°C at 10°C. After slow seedlings, the indoor temperature is maintained at 25°C - 28°C during the day, 14°C - 16°C in the first half of the night, and 12°C - 14°C in the middle of the night. The suitable ground temperature is about 22°C, which is beneficial to flower bud differentiation and plant development; during the flowering period, it is maintained at 26°C - 30°C during the day and 18°C - 13°C at night, with a ground temperature of 18°C - 20°C; during the fruit setting period, 26°C - 30°C during the day and 15°C - 18°C at night; during the fruit expansion period, 25°C - 28°C during the day, 18°C - 14°C in the first half of the night, and 14°C - 12°C in the middle of the night, and the air humidity is preferably 50% - 65%. When the lowest external temperature drops to 12°C, it is the critical temperature index for sealing the shed at night. Tomato fruits can be harvested in early December. In winter, it is necessary to pay attention to keeping warm, and the solar greenhouse can be covered with insulation or straw.

[0053] (2) Water and fertilizer management: After planting, the outdoor temperature is high. It is recommended to use small water droplets to reduce the ground temperature. Generally, drip irrigation is carried out once every morning or evening for 60 minutes each time, about 2 cubic meters of water per acre, while ditches are generally watered 1 - 7 days in the morning or evening. When the first ear extends to a diameter of 4 - 5 cm and the third ear flower has been completed. When the fourth ear blooms, water and fertilizer are applied, and the fruits are set. Apply 10 kg of potassium sulfate compound fertilizer per acre with a dark ditch, water-soluble fertilizer with a total nutrient of 6 kg every 15 - 20 days (nitrogen: phosphorus: potassium = 15:15:15), water 8 kg, water once every 7 - 10 days, or apply 10 kg of urea and 10 kg of compound fertilizer with a total nutrient per acre by drip irrigation, and irrigate 2 cubic meters of water.

[0054] (3) Light management: It is cultivated in a greenhouse in winter and spring. During the growing period, from November to February, it will have to experience a period of low temperature, weak light, short sunshine, rainy and snowy weather. Select an EVA multi-functional film with good light transmittance to cover the greenhouse, and promptly remove dust, snow, etc. on the film with a mop.

[0055] As an implementation manner of the embodiment of the present application, based on every 1 kg of dry soil, the addition amount of the plant activator is 10 - 20 g.

[0056] In some specific embodiments, based on every 1 kg of dry soil, the addition amount of the plant activator can be 10 g, 11 g, 12 g, 13 g, 14 g, 15 g, 16 g, 17 g, 18 g, 19 g, 20 g, etc.

[0057] The features and performance of the present invention will be further described in detail below in conjunction with the embodiments.

[0058] Example 1

[0059] This example provides a plant activator. The active ingredient of the plant activator is composed of hinokitiol and rapeseed pollen extract with a weight ratio of 4:1. The preparation method of the plant activator includes the following steps:

[0060] Add 4 g of hinokitiol and 1 g of rapeseed pollen extract to 1 L of ethanol and stir to mix to obtain the plant activator; wherein, the rapeseed pollen extract is a commercially available product purchased from Xi'an Ruili Biotechnology Co., Ltd.

[0061] Example 2

[0062] This example provides a plant activator, which is only different from Example 1 in that: the preparation method of the rapeseed pollen extract includes the following steps: Mix the rapeseed pollen after wall-breaking treatment and an ethanol aqueous solution with a mass fraction of 65% according to a weight ratio of 1:10 to obtain a mixed solution; Place the mixed solution in a water bath at a temperature of 60 °C for ultrasonic extraction (ultrasonic frequency is 35 kHz, power is 800 W) for 50 minutes, and then filter and evaporate and concentrate to obtain a crude rapeseed pollen alcohol extract; Pass the crude rapeseed pollen alcohol extract through an AB-8 macroporous resin column, first elute with an ethanol aqueous solution with a mass fraction of 2.5% at a flow rate of 2.5 times the column volume per hour, the elution volume is 3 times the column volume, and discard the first eluate; Then elute with an ethanol aqueous solution with a mass fraction of 82% at a flow rate of 2.5 times the column volume per hour, the elution volume is 6 times the column volume, and collect the second eluate; Concentrate and dry the second eluate to obtain the rapeseed pollen extract.

[0063] Example 3

[0064] This example provides a plant activator, which is only different from Example 2 in that: the active ingredient of the plant activator is composed of rapeseed pollen extract and hinokitiol with a weight ratio of 4:1; specifically, adjust the dosage of rapeseed pollen extract to 4 g and the dosage of hinokitiol to 1 g.

[0065] Example 4

[0066] This example provides a plant activator, which is only different from Example 2 in that: the active ingredient of the plant activator is composed of hinokitiol and rapeseed pollen extract with a weight ratio of 7:1; specifically, adjust the dosage of hinokitiol to 7 g and the dosage of rapeseed pollen extract to 1 g.

[0067] Example 5

[0068] This example provides a plant activator, which is only different from Example 2 in that: the active ingredient of the plant activator is composed of hinokitiol and rapeseed pollen extract with a weight ratio of 6:1; specifically, the dosage of hinokitiol is adjusted to 6 g, and the dosage of rapeseed pollen extract is adjusted to 1 g.

[0069] Comparative Example 1

[0070] This example provides a plant activator, which is only different from Example 5 in that: the active ingredient of the plant activator is all hinokitiol; specifically, the dosage of hinokitiol is adjusted to 7 g, and the dosage of rapeseed pollen extract is adjusted to 0 g.

[0071] Comparative Example 2

[0072] This example provides a plant activator, which is only different from Example 5 in that: the active ingredient of the plant activator is all rapeseed pollen extract; specifically, the dosage of hinokitiol is adjusted to 0 g, and the dosage of rapeseed pollen extract is adjusted to 7 g.

[0073] Test Example

[0074] In this example, the plant activators obtained in Examples 1 - 5 and Comparative Examples 1 - 2 were subjected to effect tests.

[0075] Test materials: The tomato variety is Baiguo Qiangfeng; the potting soil has been previously sun-dried, sieved, and sterilized with a high-temperature steam boiler.

[0076] Test groups: Blank control group (no plant activator was used for soil mixing treatment); Experimental group 1 (soil mixing treatment was carried out with the plant activator obtained in Example 1); Experimental group 2 (soil mixing treatment was carried out with the plant activator obtained in Example 2); Experimental group 3 (soil mixing treatment was carried out with the plant activator obtained in Example 3); Experimental group 4 (soil mixing treatment was carried out with the plant activator obtained in Example 4); Experimental group 5 (soil mixing treatment was carried out with the plant activator obtained in Example 5); Experimental group 6 (soil mixing treatment was carried out with the plant activator obtained in Comparative Example 1); Experimental group 7 (soil mixing treatment was carried out with the plant activator obtained in Comparative Example 2).

[0077] Test method: 1) Soil mixing treatment: The plant activators obtained in Examples 1-6 and Comparative Examples 1-2 were respectively mixed with potting soil and volatilized fully for about one to two weeks to obtain planting soil containing the plant activator; among them, the dosage of the plant activator was 30 g, and the dosage of the potting soil was 3 kg. 2) Transplant tomato seedlings with three leaves and one heart and consistent growth vigor into the said planting soil containing the plant activator, and then carry out maintenance management. 3) Determination of tomato single-plant yield: The tomato was topped after leaving four spikes, the number, fruit weight and total mass of each tomato plant were recorded, and the average single-plant yield = total mass of four plants / 4. The determination results are shown in Table 1. Determination of tomato quality: The anthrone colorimetric method was used for the determination of soluble sugar, and the determination results are shown in Table 2.

[0078] Table 1

[0079]

[0080] Table 2

[0081]

[0082] In summary, the embodiments of the present application provide a plant activator, the active ingredients of which include hinokitiol and rapeseed pollen extract. For the first time, hinokitiol and rapeseed pollen extract are used in combination as the active ingredients of the plant activator. The two cooperate with each other and jointly play a role in promoting the self-growth of crops, and can significantly improve the yield and quality of crops such as tomatoes. At the same time, the use of chemical drugs can be reduced, which is of great significance for improving the quality and efficiency of crops and the sustainable development of agriculture.

[0083] The various embodiments of the present application may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be construed as a rigid limitation on the scope of the present application; therefore, it should be considered that the described range description has specifically disclosed all possible sub-ranges and single values within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. In addition, whenever a numerical range is indicated herein, it means including any cited number (fraction or integer) within the indicated range.

[0084] In addition, in the description of the specification of the present application, the terms "include", "comprise", etc. mean "including but not limited to". In this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations.

[0085] The embodiments described above are some, but not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

Claims

1. Use of a plant activator in increasing tomato yield, characterized in that, The active ingredients in the plant activator include hinokitiol and rapeseed pollen extract; The weight ratio of the hinokitiol to the rapeseed pollen extract is (4-7):1; The rapeseed pollen extract is an alcohol extract of rapeseed pollen; The preparation method of the alcohol extract of rapeseed pollen includes the following steps: Mix the wall-broken rapeseed pollen and an ethanol aqueous solution with a mass fraction of 60%-70% according to a weight ratio of 1:(8-12) to obtain a mixed solution; Place the mixed solution in a water bath at a temperature of 55-65°C for ultrasonic extraction for 40-60 minutes, then filter and evaporate and concentrate to obtain a crude alcohol extract of rapeseed pollen; Pass the crude alcohol extract of rapeseed pollen through a macroporous resin column. First, elute with an ethanol aqueous solution with a mass fraction of 2%-3% at a flow rate of 2-3 times the column volume per hour, and the elution volume is 3 times the column volume. Discard the first eluate; then elute with an ethanol aqueous solution with a mass fraction of 80%-85% at a flow rate of 2-3 times the column volume per hour, and the elution volume is 5-8 times the column volume. Collect the second eluate; Concentrate and dry the second eluate to obtain an alcohol extract of rapeseed pollen.

2. The application according to claim 1, characterized in that The working parameters of the ultrasonic extraction include: the ultrasonic frequency is 30-40 kHz and the power is 700-850 W.

3. The application according to claim 1, characterized in that, The plant activator also includes an alcohol solvent.

4. The application according to claim 1, characterized in that, Calculated by weight percentage, the concentration of the active ingredients in the plant activator is 5-8 g / L.

5. A method for improving the quality and yield of tomatoes, characterized in that, The method includes the following steps: Add the plant activator according to any one of claims 1-4 to the planting soil and mix to obtain the planting soil containing the plant activator; Transplant the tomato seedlings into the planting soil containing the plant activator, and then carry out maintenance management.

6. The method for improving the quality and yield of tomatoes according to claim 5, characterized in that, Calculated by every 1 kg of dry soil, the addition amount of the plant activator is 10-20 g.

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