Plant moisturizing lotion and method of making same

Abstract

The present application relates to the technical field of skin care products, and particularly relates to a plant moisturizing lotion and a preparation method thereof.A plant moisturizing lotion comprises the following components in parts by weight: solvent 70-85 parts, humectant 1-10 parts, emollient 5-20 parts, emulsifier 2-10 parts, thickening agent 0.1-1 part and chelating agent 0.01-0.1 part; the humectant comprises stem extract of dendrobium and extract of Galanthus orbiculatus in a mass ratio of (1-5):(1-5). The plant moisturizing lotion of the present application can effectively improve the moisture content of the skin by matching the components, has the effects of resisting primary marks and repairing the skin barrier, and has excellent heat resistance and cold resistance, and a refreshing and smooth skin feel.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, and in particular to a plant-based moisturizing lotion and its preparation method. Background Technology

[0002] Skin is a living tissue that relies on moisture to achieve optimal function and performance. Sufficient skin hydration plays a crucial role in skin health; adequate hydration promotes enzyme reactions, accelerates the maturation of the stratum corneum, and maintains its elasticity. Infants have a high hydration level in their stratum corneum, resulting in smooth, delicate, and elastic skin. Skin hydration decreases with age. When the stratum corneum's hydration level drops to 10%, it leads to dryness, dullness, reduced elasticity, and increased wrinkles—signs of skin aging. Simultaneously, it weakens the skin barrier function, making it easier for harmful external substances to penetrate, potentially leading to skin diseases. Therefore, moisturizing plays an indispensable role in cosmetic skincare products.

[0003] Moisturizing lotions contain a certain amount of water to replenish skin moisture, and also contain a small amount of oil to further hydrate the skin. Sodium hyaluronate, allantoin, betaine, and trehalose are common additives in moisturizing cosmetics. Due to the increasing demand for natural, non-irritating, and side-effect-free cosmetics, the application of natural plants in skincare products is gradually increasing. Most ordinary lotions tend to have a relatively simple moisturizing function, and often overlook consumers' demands regarding the feel and experience of moisturizing creams.

[0004] Based on the above issues, it is essential to develop a plant-based skincare lotion that has multiple functions, a smooth and moisturizing feel, and is quickly absorbed, so that the product can moisturize while fighting initial signs of aging and improving the skin barrier function.

[0005] In view of this, the present invention is hereby proposed. Summary of the Invention

[0006] One object of the present invention is to provide a plant-based moisturizing lotion that can effectively increase the skin's moisture content and provide a refreshing and smooth skin feel.

[0007] Another object of the present invention is to provide a method for preparing plant-based moisturizing emulsions.

[0008] To achieve the above-mentioned objectives of the present invention, one aspect of the present invention provides a plant-based moisturizing emulsion, comprising the following components in parts by weight:

[0009] The solvent consists of 70-85 parts, humectant 1-10 parts, emollient 5-20 parts, emulsifier 2-10 parts, thickener 0.1-1 parts, and chelating agent 0.01-0.1 parts.

[0010] The moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (1-5):(1-5).

[0011] In a specific embodiment of the present invention, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (3.5-4.5):(3-4). Further, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of 4:3.5.

[0012] In a specific embodiment of the present invention, the Dendrobium stem extract is an aqueous extract of Dendrobium stem, and the Hydrangea extract is an aqueous extract of Hydrangea fungus.

[0013] In a specific embodiment of the present invention, the molecular weight of the Dendrobium stem extract is 10 wDa to 50 wDa.

[0014] In a specific embodiment of the present invention, the moisturizer further includes sodium hyaluronate. Further, the content of sodium hyaluronate in the moisturizer is 0.05 wt% to 0.5 wt%, preferably 0.1 wt% to 0.2 wt%.

[0015] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0016] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized, deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m), and the filtrate was collected.

[0017] (b) The filtrate and water were used to extract *Hydrangea macrophylla* at a material-to-liquid ratio of 1:(10-100)(m / m), and the extract was then decolorized, deodorized, and filtered.

[0018] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0019] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized and deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m). The extract was separated by membrane separation and Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa was collected.

[0020] (b) The extract of *Hydrangea macrophylla* was carried out using Dendrobium polysaccharide extract and water at a material-to-liquid ratio of 1:(10-100)(m / m). The extract was then decolorized, deodorized, and filtered.

[0021] In a specific embodiment of the present invention, the extraction temperature is 40–90°C; the extraction time is 1–4 hours.

[0022] In a specific embodiment of the present invention, the *Hydrangea macrophylla* is subjected to high-temperature micro-pressure or steam treatment before extraction.

[0023] In a specific embodiment of the present invention, the preparation of the moisturizer further includes: mixing the mixture of the Dendrobium stem extract and the Hydrangea macrophylla extract with sodium hyaluronate in a certain proportion, and then subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment; or, mixing the Dendrobium stem extract with sodium hyaluronate in a certain proportion, subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment, and then mixing it with the Hydrangea macrophylla extract.

[0024] In a specific embodiment of the present invention, the solvent includes water;

[0025] The emollients include one or more of hydrogenated polyisobutylene, caprylic / capric triglyceride, polydimethylsiloxane, polydimethylsiloxane alcohol, squalane, glycerides, jojoba esters, tocopherol, phytosterols, macadamia oleate, avocado extract, and tocopheryl acetate.

[0026] The emulsifier includes any one or more of behenol, sucrose stearate, cetyl alcohol, cocoyl glucoside, glyceryl stearate, PEG-100 stearate, behenol, sucrose stearate, sodium stearoyl lactylate, hydrogenated lecithin, cetearyl alcohol, and polyglycerol-3-methylglucose diisostearate.

[0027] The thickener includes any one or more of the following: acrylate / C10-30 alkanol acrylate crosspolymers, xanthan gum, carbomer, high molecular weight cellulose, gum arabic, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, and acrylates.

[0028] The chelating agent includes disodium EDTA;

[0029] The plant-based moisturizing emulsion further includes 0.1 to 1 part by weight of a preservative; the preservative includes any one or more of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, methylparaben, benzyl alcohol, benzoic acid, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea, and iodopropynyl butylcarbamate.

[0030] Another aspect of the present invention provides a method for preparing a plant-based moisturizing emulsion, comprising the following steps:

[0031] (1) Mix the humectant, thickener and chelating agent in a solvent and heat to 80-82°C, then keep warm.

[0032] (2) Mix the emollient and emulsifier and heat to 80-82°C. Stir and melt into a liquid. Add the mixture to the material obtained in step (1) to obtain a mixture.

[0033] (3) Homogenize the mixture until it is uniformly emulsified, defoam and cool down; when it is cooled to 70-75℃, homogenize it until uniform; then cool it down to 60-65℃, keep it warm and stir to obtain plant moisturizing emulsion.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0035] (1) The plant-based moisturizing emulsion of the present invention can effectively increase the skin's moisture content through the combination of its components, and has the effects of anti-scarring and repairing the skin barrier. It also has excellent heat and cold resistance, as well as a refreshing and smooth skin feel. Among them, the moisturizing agent is a combination of Dendrobium and Hydrangea macrophylla, which can endogenously promote the production of hyaluronic acid, promote the expression of aquaporin AQP3, promote the synthesis of tight junction protein Claudin-1 and filaggrin FLG, and promote the operation of the skin's own moisturizing system to achieve moisturizing effect.

[0036] (2) The preparation method of the plant moisturizing emulsion of the present invention is simple to operate and the conditions are mild. Attached Figure Description

[0037] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0038] Figure 1 The results of the skin elasticity test of this invention show the changes in crow's feet wrinkles.

[0039] Figure 2 The rheological test results are for single solutions and combined solutions of sodium hyaluronate and Dendrobium polysaccharide of the present invention.

[0040] Figure 3 The results show the moisture absorption rate of single and combined samples of sodium hyaluronate and Dendrobium polysaccharide according to the present invention. Detailed Implementation

[0041] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0042] This invention provides a plant-based moisturizing emulsion, comprising the following components in parts by weight:

[0043] The solvent consists of 70-85 parts, humectant 1-10 parts, emollient 5-20 parts, emulsifier 2-10 parts, thickener 0.1-1 parts, and chelating agent 0.01-0.1 parts.

[0044] The moisturizers include Dendrobium stem extract and Hydrangea extract in a mass ratio of (1-5):(1-5).

[0045] The plant-based moisturizing emulsion of this invention, through the combination of its various components, can effectively increase the skin's moisture content, while also having anti-aging and skin barrier repair effects. It also has excellent heat and cold resistance, as well as a refreshing and smooth skin feel. Among them, the moisturizing agent uses a combination of Dendrobium and Hydrangea macrophylla, which can endogenously promote the production of hyaluronic acid, promote the expression of aquaporin AQP3, promote the synthesis of tight junction protein Claudin-1 and filaggrin FLG, and promote the operation of the skin's own moisturizing system to achieve moisturizing effect.

[0046] In different embodiments, the amounts of each component in the plant-based moisturizing emulsion, by weight, can be exemplarily exemplified as follows:

[0047] The amount of solvent can be 70 parts, 72 parts, 75 parts, 78 parts, 80 parts, 82 parts, 85 parts, or any combination thereof;

[0048] The amount of moisturizer used can be 1 part, 2 parts, 5 parts, 8 parts, 10 parts, or any combination thereof;

[0049] The amount of moisturizer used can be 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, or any combination thereof;

[0050] The amount of emulsifier can be 2 parts, 5 parts, 8 parts, 10 parts, or any combination thereof;

[0051] The amount of thickener can be 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, or any combination thereof;

[0052] The amount of chelating agent can be 0.01 parts, 0.02 parts, 0.05 parts, 0.08 parts, 0.1 parts, or any combination thereof.

[0053] In different embodiments, the mass ratio of Dendrobium stem extract and Hydrangea extract in the humectant can be 1:1, 1:2, 1:3, 1:4, 1:5, 2:1, 2:3, 2:5, 3:1, 3:2, 3:4, 3:5, 4:1, 4:3, 4:5, 5:1, 5:2, 5:3, 5:4, or any combination thereof.

[0054] In a specific embodiment of the present invention, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (3.5-4.5):(3-4). Further, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of 4:3.5.

[0055] It should be noted that in the moisturizer of the present invention, the amounts of Dendrobium stem extract and Hydrangea extract are calculated based on the amounts of the raw materials Dendrobium stem and Hydrangea used.

[0056] Extraction using Dendrobium stems and Hydrangea macrophylla in the above ratio is more conducive to promoting the production of hyaluronic acid endogenously, promoting the expression of aquaporin AQP3, and promoting the synthesis of tight junction protein Claudin-1 and filaggrin FLG.

[0057] In a specific embodiment of the present invention, the Dendrobium stem extract is an aqueous extract of Dendrobium stem, and the Hydrangea extract is an aqueous extract of Hydrangea fungus.

[0058] In a specific embodiment of the present invention, the molecular weight of Dendrobium polysaccharide in the Dendrobium stem extract is 10 wDa to 50 wDa.

[0059] In different embodiments, the molecular weight of the Dendrobium polysaccharide in the Dendrobium stem extract can be within the range of 10 wDa, 15 wDa, 20 wDa, 25 wDa, 30 wDa, 35 wDa, 40 wDa, 45 wDa, 50 wDa, or any combination thereof.

[0060] Using Dendrobium polysaccharides within the above molecular weight range, it has been verified through moisturizing efficacy tests and skin feel evaluations that they have the best effect on promoting AQP3 expression and skin feel.

[0061] In a specific embodiment of the present invention, the moisturizer further includes sodium hyaluronate. Further, the content of sodium hyaluronate in the moisturizer is 0.05 wt% to 0.5 wt%, preferably 0.1 wt% to 0.2 wt%.

[0062] In various embodiments, the content of sodium hyaluronate in the humectant may be a range of 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.25 wt%, 0.3 wt%, 0.35 wt%, 0.4 wt%, 0.45 wt%, 0.5 wt%, or any combination thereof.

[0063] Sodium hyaluronate is used, which can form supramolecular interactions with Dendrobium polysaccharides to form a corresponding network structure, which has the effects of water capture, slow release and deep penetration.

[0064] The molecular weight of sodium hyaluronate can be adjusted according to actual needs. For example, the molecular weight of sodium hyaluronate can be 1000 Da to 200 wDa, such as 1000 Da, 5000 Da, 1 wDa, 5 wDa, 10 wDa, 20 wDa, 50 wDa, 100 wDa, 200 wDa, or any combination thereof.

[0065] In a specific embodiment of the present invention, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract is (1-6):1.

[0066] In different embodiments, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract can be 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1, 5.5:1, 6:1, or any combination thereof.

[0067] When the content of sodium hyaluronate in the moisturizer meets the above requirements, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract is (1-6):1. For example, when the content of sodium hyaluronate is 0.15wt%, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the obtained moisturizer is close to 5:2.

[0068] In a specific embodiment of the present invention, Dendrobium includes Dendrobium officinale.

[0069] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0070] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized, deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m), and the filtrate was collected.

[0071] (b) The filtrate and water were used to extract *Hydrangea macrophylla* at a material-to-liquid ratio of 1:(10-100)(m / m), and the extract was then decolorized, deodorized, and filtered.

[0072] In a specific embodiment of the present invention, the preparation of the humectant includes:

[0073] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized and deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m). The extract was separated by membrane separation and Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa was collected.

[0074] (b) The extract of *Hydrangea macrophylla* was carried out using Dendrobium polysaccharide extract and water at a material-to-liquid ratio of 1:(10-100)(m / m). The extract was then decolorized, deodorized, and filtered.

[0075] In the preparation method of the above-mentioned moisturizer, water is used as the extraction solvent. First, the Dendrobium stem is extracted, and the Dendrobium stem extract is separated by membrane separation to collect the Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa. Then, the Dendrobium polysaccharide extract and water are used as extraction solvents to extract Hydrangea macrophylla. In different embodiments, in step (a), the feed-liquid ratio can be 1:10 (m / m), 1:20 (m / m), 1:30 (m / m), 1:40 (m / m), 1:50 (m / m), 1:60 (m / m), 1:70 (m / m), 1:80 (m / m), or any combination thereof; in step (b), the feed-liquid ratio can be 1:10 (m / m), 1:20 (m / m), 1:30 (m / m), 1:40 (m / m), 1:50 (m / m), 1:60 (m / m), 1:70 (m / m), 1:80 (m / m), 1:90 (m / m), 1:100 (m / m), or any combination thereof.

[0076] It should be noted that in step (b), Dendrobium polysaccharide extract (or filtrate) and water are used together as extraction solvents to extract Hydrangea macrophylla. The material-liquid ratio refers to the ratio of the mass of Hydrangea macrophylla relative to the mass of Dendrobium polysaccharide extract (or filtrate) and water.

[0077] In another specific embodiment of the present invention, the preparation of the moisturizer includes: extracting Dendrobium stems and Hydrangea macrophylla separately using water as the extraction solvent and a material-to-liquid ratio of 1:(10-100)(m / m); decolorizing and deodorizing the extract and filtering it; and then mixing and compounding the Dendrobium stem extract and Hydrangea macrophylla extract.

[0078] In a specific embodiment of the present invention, the extraction temperature is 40–90°C; the extraction time is 1–4 hours.

[0079] For the above extraction method, the extraction temperature can be 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃, 85℃, 90℃ or any combination thereof, and the extraction time can be 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h or any combination thereof.

[0080] In a specific embodiment of the present invention, decolorization and deodorization include: treating the extract with activated carbon or hydrogen peroxide, and collecting the filtrate. Further, the amount of activated carbon added is 0.1 wt% to 2 wt% of the extract; the amount of hydrogen peroxide added is 0.01 wt% to 1 wt% of the extract.

[0081] In a specific embodiment of the present invention, the filtration process includes coarse filtration and fine filtration. Further, the coarse filtration includes paperboard filtration; the fine filtration includes both paperboard filtration and diatomaceous earth filtration.

[0082] In a specific embodiment of the present invention, the *Hydrangea macrophylla* is subjected to high-temperature micro-pressure or steam treatment before extraction. Further, the high-temperature micro-pressure treatment includes: a temperature of 110–121°C, a pressure of 0.15–0.23 MPa, and a micro-pressure time of 5–20 min; the steam treatment includes: steaming at 90–100°C for 5–20 min. Wherein, the pressure in the high-temperature micro-pressure treatment refers to absolute pressure, which is the sum of gauge pressure (relative pressure) and atmospheric pressure.

[0083] In different embodiments, the high-temperature micro-pressure treatment can be performed at temperatures ranging from 110°C, 112°C, 114°C, 115°C, 116°C, 118°C, 120°C, 121°C, or any combination thereof; the pressure can be ranging from 0.15MPa, 0.16MPa, 0.18MPa, 0.2MPa, 0.22MPa, 0.23MPa, or any combination thereof; and the treatment time can be ranging from 5min, 8min, 10min, 12min, 15min, 18min, 20min, or any combination thereof.

[0084] High-temperature micro-pressure or steam treatment helps to promote the extraction of active ingredients from *Hydrangea macrophylla*, thereby further enhancing the moisturizing ability of the resulting moisturizer.

[0085] In a specific embodiment of the present invention, the preparation of the moisturizer further includes: mixing a mixture of Dendrobium stem extract and Hydrangea macrophylla extract with sodium hyaluronate in a certain proportion, and then subjecting it to homogenization, microwave, ultrasonic, and / or micro-pressure treatment. The Dendrobium stem extract may be Dendrobium stem extract without membrane separation, or Dendrobium polysaccharide extract with membrane separation.

[0086] In another specific embodiment of the present invention, the preparation of the moisturizer further includes: mixing Dendrobium stem extract with sodium hyaluronate in a certain proportion, and subjecting the mixture to homogenization, microwave, ultrasonic and / or micro-pressure treatment; and then mixing it with Hydrangea macrophylla extract.

[0087] The homogenization conditions may include: a rotation speed of 2000–5000 rpm and a homogenization time of 5–30 min; the micro-pressure conditions may include: a temperature of 110–121℃, a pressure of 0.15–0.23 MPa, and a micro-pressure time of 5–20 min; the microwave conditions may include: a microwave power of 200–800 W and a microwave time of 5–20 min; and the ultrasonic conditions may include: an ultrasonic power of 50–400 W, an ultrasonic temperature of 30–60℃, and an ultrasonic time of 5–20 min. The pressure in the micro-pressure treatment refers to absolute pressure, which is the sum of gauge pressure (relative pressure) and atmospheric pressure.

[0088] In a specific embodiment of the present invention, the solvent includes water;

[0089] Emollients include any one or more of hydrogenated polyisobutylene, caprylic / capric triglyceride, polydimethicone, polydimethicone alcohol, squalane, glycerides, jojoba esters, tocopherol, phytosterols, macadamia oleate, avocado extract, and tocopheryl acetate.

[0090] The emulsifiers include any one or more of behenol, sucrose stearate, cetyl alcohol, cocoyl glucoside, glyceryl stearate, PEG-100 stearate, sodium stearoyl lactylate, hydrogenated lecithin, cetearyl alcohol, and polyglycerol-3-methylglucose diisostearate.

[0091] Thickeners include any one or more of the following: acrylate / C10-30 alkanol acrylate crosspolymers, xanthan gum, carbomer, high molecular weight cellulose, gum arabic, sodium acrylate / sodium acryloyl dimethyl taurate copolymer, and acrylates.

[0092] Chelating agents include disodium EDTA;

[0093] Plant-based moisturizing emulsions may also include 0.1 to 1 part by weight of preservatives; the preservatives include any one or more of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, methylparaben, benzyl alcohol, benzoic acid, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea and iodopropynyl butylcarbamate.

[0094] In different embodiments, the amount of preservative can be 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, or any combination thereof.

[0095] The plant-based moisturizing emulsion of the present invention is not limited to one type of component, but can be formulated with multiple components.

[0096] Another aspect of the present invention provides a method for preparing a plant-based moisturizing emulsion, comprising the following steps:

[0097] (1) Mix the humectant, thickener and chelating agent in a solvent and heat to 80-82°C, then keep warm.

[0098] (2) Mix the emollient and emulsifier and heat to 80-82°C. Stir and melt into a liquid. Add the mixture to the material obtained in step (1) to obtain a mixture.

[0099] (3) Homogenize the mixture until it is uniformly emulsified, defoam and cool down; when it is cooled to 70-75℃, add the preservative and homogenize until uniform; then cool down to 60-65℃, keep warm and stir to obtain plant moisturizing emulsion.

[0100] In a specific embodiment of the present invention, in step (1), the heat preservation time is 20-25 minutes. Further, homogenization can be performed for 1-2 minutes before the heat preservation process.

[0101] In a specific embodiment of the present invention, in step (3), the homogenization process is carried out until the emulsion is uniform, and the homogenization time is 3 to 5 minutes; after adding the preservative, the homogenization time is 1 to 2 minutes; and the heat preservation and stirring process is carried out for 20 to 25 minutes.

[0102] The raw material information used in the following specific embodiments may be as follows, but is not limited to:

[0103] Betaine: Supplier is Weisbo; Glycerin: Supplier is Sven; Aloe extract: Supplier is Beijing Dongfang Miaosen Biotechnology Co., Ltd.; Hydrogenated polyisobutylene: Supplier is Lebaokang; Caprylic / capric triglyceride: Supplier is Croda; Polydimethylsiloxane: Supplier is Dow Corning; Polydimethylsiloxane alcohol: Supplier is Dow Corning; Cetyl alcohol: Supplier is Miaosen; Cocoyl glucoside: Supplier is Miaosen; Glyceryl stearate: Supplier is Croda; PEG-100 stearate: Supplier is Croda; Sodium stearoyl lactylate: Supplier is Muqi; Hydrogenated lecithin: Supplier is NIKKOL; Acrylic (ester) crosspolymer / C10-30 alkanol acrylate crosspolymer: Supplier is Lubrizol; Carbomer: Supplier is Lubrizol; Xanthan gum: Supplier is Rhodia; Disodium EDTA: Supplier is AkzoNobel; 1,2-Hexanediol: Supplier is Rentai; 1,2-Pentanediol: Supplier is Huayu; p-Hydroxyacetophenone, phenoxyethanol and ethylhexylglycerin: Supplier is THOR.

[0104] Examples 1-8

[0105] Examples 1-8 of this invention provide plant-based moisturizing emulsions and their preparation methods. The raw material composition of the plant-based moisturizing emulsions is shown in Table 1. The specific preparation methods include the following steps:

[0106] (1) Soak the humectant, thickener and chelating agent in a preparation pot containing solvent water, heat to 80-82°C until completely dissolved and homogenized, homogenize for 1-2 minutes, keep warm for 20 minutes to sterilize and defoam, and set aside.

[0107] (2) Mix the emollient and emulsifier and heat to 80-82°C. Stir and melt into a liquid, then add to the preparation pot in step (1).

[0108] (3) Turn on the homogenizer and homogenize for 3-5 minutes until the emulsion is completely homogeneous. After defoaming, cool down. Cool down to 70-75℃, add preservative, and homogenize at low speed for 1-2 minutes until homogeneous. Then cool down to 60℃, keep warm and stir for 20 minutes until homogeneous to obtain plant moisturizing emulsion.

[0109] Table 1. Raw material composition (parts by weight) of different plant-based moisturizing emulsions

[0110]

[0111]

[0112] Among them, emollient A includes jojoba esters, tocopherols, phytosterols, macadamia oleate, and avocado extract in a mass ratio of 2:2:3:3:2.

[0113] Emollient B comprises hydrogenated polyisobutylene, caprylic / capric triglyceride, polydimethylsiloxane, avocado extract and tocopheryl acetate in a mass ratio of 2:6:2:2:1.

[0114] Emulsifier A includes behenol, sucrose stearate, polyglycerol-3-methylglucose diisostearate, and hydrogenated lecithin in a mass ratio of 1:1:1:0.5.

[0115] Emulsifier B comprises cocoyl glucoside, sucrose stearate, and sodium stearoyl lactylate in a mass ratio of 2:2:1;

[0116] Thickener A comprises carbomer and xanthan gum in a mass ratio of 1:1;

[0117] Thickener B is an acrylate / C10-30 alkanol acrylate crosslinked polymer;

[0118] Preservative A comprises 1,2-hexanediol and p-hydroxyacetophenone in a mass ratio of 1:1;

[0119] Preservative B comprises phenoxyethanol and ethylhexylglycerin in a mass ratio of 9:1.

[0120] The preparation method of moisturizer A includes:

[0121] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0122] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter and collect the filtrate.

[0123] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 10wDa and 50wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa is collected.

[0124] (d) Add sodium hyaluronate to the Dendrobium polysaccharide extract obtained in step (c) to obtain a compound solution; homogenize the compound solution in a homogenizer at 2500 rpm for 10 min.

[0125] (e) Extraction of Hydrangea macrophylla: Hydrangea macrophylla was mixed at a material-to-liquid ratio of 1:60 (m / m), stirred at room temperature for 30 min, and then extracted at 60℃ for 2 h. After extraction, 0.5 wt% activated carbon was added for decolorization and deodorization treatment, followed by fine filtration and collection of filtrate.

[0126] (f) Compounding: The compound solution after homogenization in step (d) is mixed with the filtrate obtained in step (e), and 23% glycerol (by mass fraction of the mixture) is added to obtain the mixture.

[0127] (g) Sterilization: Add 1% 1,2-pentanediol and 1.5% 1,2-hexanediol (by mass fraction of the final product) to the material obtained in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0128] In step (d), the amount of sodium hyaluronate used is 0.15% of the combined mass of the Dendrobium polysaccharide extract in step (c), the Hydrangea spp. extract filtrate obtained in step (e), and the glycerol in step (f).

[0129] The preparation of moisturizer B includes:

[0130] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0131] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter and collect the filtrate.

[0132] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 10wDa and 50wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa is collected.

[0133] (d) Add a certain amount of water to the Dendrobium polysaccharide extract obtained in step (c) (use all of the Dendrobium polysaccharide extract and make up the rest with water), and mix it with Hydrangea macrophylla at a material-to-liquid ratio of 1:60 (m / m). Stir at room temperature for 30 minutes, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze and collect the filtrate.

[0134] (e) Filtration: The filtrate obtained in step (d) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0135] (f) Compounding: Add a certain amount of glycerol to the filtrate obtained in step (e) to obtain a compound solution.

[0136] (g) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0137] In step (f), the mass fraction of glycerol in the compound solution is 23%; in step (g), the mass fractions of 1,2-pentanediol and 1,2-hexanediol in the final product are 1% and 1.5%, respectively.

[0138] Example 9

[0139] This embodiment provides a plant-based moisturizing emulsion and its preparation method. Referring to Embodiment 1, the only difference is that moisturizer A is replaced with an equal weight of moisturizer C.

[0140] The preparation method of humectant C used in this embodiment includes:

[0141] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0142] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter with gauze and collect the filtrate.

[0143] (c) Add a certain amount of water to the filtrate obtained in step (b) (use all the filtrate and make up the rest with water), and stir with *Hydrangea macrophylla* at a material-to-liquid ratio of 1:60 (m / m) for 30 minutes at room temperature, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze to collect the filtrate.

[0144] (d) Filtration: The filtrate obtained in step (c) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0145] (e) Compounding: Add a certain amount of glycerol to the filtrate obtained in step (d) to obtain a compound solution.

[0146] (f) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (e), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0147] In step (e), the mass fraction of glycerol in the compound solution is 23%; in step (f), the mass fractions of 1,2-pentanediol and 1,2-hexanediol in the final product are 1% and 1.5%, respectively.

[0148] Example 10

[0149] This embodiment provides a plant-based moisturizing emulsion and its preparation method. Referring to Embodiment 1, the only difference is that moisturizer A is replaced with an equal weight of moisturizer D.

[0150] The preparation methods of moisturizer D include:

[0151] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0152] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter with gauze and collect the filtrate.

[0153] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 1wDa and 10wDa membranes to collect Dendrobium polysaccharide extracts with molecular weights between 1w and 10wDa.

[0154] (d) Add a certain amount of water to the Dendrobium polysaccharide extract obtained in step (c) (use all of the Dendrobium polysaccharide extract and make up the rest with water), and mix it with Hydrangea macrophylla at a material-to-liquid ratio of 1:60 (m / m). Stir at room temperature for 30 minutes, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze and collect the filtrate.

[0155] (e) Filtration: The filtrate obtained in step (d) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0156] (f) Compounding: Add a certain amount of glycerol and sodium hyaluronate to the filtrate obtained in step (d) to obtain a compound solution.

[0157] (g) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0158] In step (f), the compound solution contains 23% glycerol and 0.15% sodium hyaluronate by mass; in step (g), 1,2-pentanediol and 1,2-hexanediol account for 1% and 1.5% of the final product by mass, respectively.

[0159] Example 11

[0160] This embodiment provides a plant-based moisturizing emulsion and its preparation method. Referring to Embodiment 1, the only difference is that moisturizer A is replaced with an equal weight of moisturizer E.

[0161] The only difference between the preparation methods of moisturizer E and moisturizer D is step (c).

[0162] In the preparation of moisturizer E, step (c) is as follows: the filtrate obtained in step (b) is filtered using 1000Da and 1wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 1000Da and 1wDa is collected.

[0163] Comparative Example 1

[0164] Comparative Example 1 provides a moisturizing emulsion and its preparation method, referring to Example 1, except that moisturizer A is replaced with an equal weight of moisturizer F.

[0165] Moisturizer F comprises betaine and glycerin in a mass ratio of 1:4.

[0166] Comparative Example 2

[0167] Comparative Example 2 provides a moisturizing emulsion and its preparation method, referring to Example 1, except that moisturizer A is replaced with an equal weight of moisturizer G.

[0168] Moisturizer G is aloe vera extract.

[0169] Comparative Examples 3-6

[0170] Comparative Examples 3-6 provide moisturizing emulsions and their preparation methods, referring to Example 1, except that the raw material composition of the moisturizing emulsions is different from that of Example 1.

[0171] The raw material composition of the moisturizing emulsions in Comparative Examples 3 to 6 is shown in Table 2.

[0172] Table 2. Raw material composition (parts by weight) of different moisturizing lotions

[0173]

[0174] Emulsifier C comprises SIMULGEL FL and EMT-10 (supplier: SEPPIC) in a mass ratio of 0.3:0.2.

[0175] Comparative Example 7

[0176] Comparative Example 7 provides a moisturizing emulsion and its preparation method, referring to Example 1, except that moisturizer A is replaced with an equal weight of moisturizer H.

[0177] The preparation of humectant H includes:

[0178] (a) Raw material pretreatment: Weigh the Dendrobium officinale stems, crush them and pass them through an 80-mesh sieve.

[0179] (b) Extraction: Dendrobium officinale stems were added to pure water at a ratio of 1:40 (m / m) and stirred at room temperature for 30 min. Then, they were extracted at 60℃ for 2 h. After extraction, 0.5% activated carbon was added for decolorization. The solution was then filtered through gauze and the filtrate was collected.

[0180] (c) Filtration: The filtrate obtained in step (b) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0181] (d) Compounding: Add 23% glycerol (mass fraction of compounded solution) to the filtrate obtained in step (c) to obtain compounded solution.

[0182] (e) Sterilization: Add 1% pentanediol and 1.5% hexanediol (by mass fraction of the final product) to the compound solution in step (d), and sterilize by stirring at 98-100°C for 40 minutes. After cooling, fill the package.

[0183] Comparative Example 8

[0184] Comparative Example 8 provides a moisturizing emulsion and its preparation method, referring to Example 1, except that moisturizer A is replaced with an equal weight of moisturizer I.

[0185] The preparation of moisturizer I includes:

[0186] (a) Raw material pretreatment: Weigh out the *Hydrangea macrophylla* and treat it under high temperature and micro pressure (110℃, 20min).

[0187] (b) Extraction: Add the *Hydrangea macrophylla* to pure water at a ratio of 1:60 (m / m), stir at room temperature for 30 min, and then extract at 60℃ for 2 h. After extraction, add 0.5% activated carbon for deodorization treatment, and then filter with gauze to collect the filtrate.

[0188] (c) Filtration: The filtrate obtained in step (b) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0189] (d) Compounding: Add 23% glycerol (mass fraction of compounded solution) to the filtrate obtained in step (c) to obtain compounded solution.

[0190] (e) Sterilization: Add 1% pentanediol and 1.5% hexanediol (by mass fraction of the final product) to the compound solution in step (d), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0191] Experimental Example 1

[0192] (1) Heat resistance test

[0193] The emulsions of the examples and comparative examples were kept at 45±2℃ for 1 month, 2 months and 3 months. After returning to room temperature, the presence or absence of oil-water separation was observed compared with before the test. ○ indicates no separation; × indicates separation.

[0194] (2) Cold resistance test

[0195] The emulsions of the examples and comparative examples were kept at -17±2℃ for 1 month, 2 months and 3 months. After returning to room temperature, they were observed to see if there was any significant difference in properties compared with before the experiment. ○ indicates no difference; × indicates a difference.

[0196] Table 3 Results of heat resistance and cold resistance tests

[0197]

[0198] The test results above show that the moisturizing emulsion of the present invention did not change significantly before and after the cold resistance and heat resistance tests, indicating that the moisturizing emulsion of the present invention has excellent cold resistance and heat resistance.

[0199] Experiment Example 2

[0200] Human body moisturizing effect test

[0201] Fifteen volunteers were selected and divided into three groups. The test samples (Examples 1-11 and Comparative Examples 1-8) of lotion were applied to different areas of the flexor surface of the volunteers' forearms. The moisture content of the corresponding areas of the volunteers' forearms was measured using a Corneometer skin moisture content analyzer before application and at 1 hour, 2 hours, 4 hours, and 8 hours after application. A higher skin moisture content value indicates higher skin hydration and better moisturizing effect. The test results are shown in Table 4.

[0202] Table 4 Results of Human Body Moisturizing Efficacy Test

[0203]

[0204]

[0205] As can be seen from the above test results, the moisturizing lotion of the present invention can significantly increase the skin's moisture content both instantly and for a long time, effectively moisturizing and increasing the skin's moisture content.

[0206] Water helps resist initial scratches and improves barrier function

[0207] The Cutometer Dual MPA580 skin elasticity analyzer was used, and the R² value was selected to characterize skin elasticity. A larger rate of change in the mean R² value indicates better skin elasticity. Changes in eye wrinkles were measured using the EvaSKIN (EOTECH, France) digital optical 3D image analyzer to evaluate the moisturizing efficacy of the samples. The Transepidermal Water Loss (TEWL) value was measured using the Aqua Flux (AF200, BIOX, UK) analyzer to characterize skin barrier function; a smaller TEWL value indicates slower transepidermal water loss and better barrier function.

[0208] Test area: Facial test (ages 25-30);

[0209] Number of participants: 20 volunteers were selected and divided into 4 groups of 5 people each. Each group used a different sample.

[0210] Test time points: week 0, week 2, week 4, week 6;

[0211] Test samples: Example 1, Example 8, Comparative Example 4, Emulsion matrix (the emulsion matrix does not contain moisturizer and is supplemented with solvent).

[0212] The test results are shown in Table 5 and Figure 1 .

[0213] Table 5 Results of Skin Elasticity Test

[0214]

[0215] The test results above show that the moisturizing lotion of the present invention can effectively improve skin elasticity, improve fine lines, enhance skin elasticity, tighten the skin, and has the effect of combating initial signs of aging with water.

[0216] Table 6 shows the results of the transdermal water loss test.

[0217] Table 6 Results of transdermal water loss test in skin

[0218]

[0219] The test results above show that the moisturizing lotion of the present invention can significantly reduce the transepidermal water loss (TEWL) value and effectively repair the skin barrier.

[0220] Sensory evaluation

[0221] Volunteers: 20 volunteers aged 18-40, each volunteer used all samples and evaluated each sample separately.

[0222] Application area: Arm

[0223] Test samples: Examples 1-11, Comparative Examples 1-8

[0224] Instructions for use: Apply 0.25mL of the corresponding lotion to different parts of the arm. The results are evaluated using a 5-point scale to assess the skin feel after use.

[0225] 4-5 points: The feeling is very obvious;

[0226] 3-4 points: The feeling is quite obvious;

[0227] 2-3 points: Mild sensation;

[0228] 1-2 points: No relevant feelings.

[0229] The test results are shown in Table 7.

[0230] Table 7 Sensory Evaluation Results

[0231]

[0232] The sensory test results above indicate that the emulsion formula of this invention uses specific moisturizing plant extracts, providing excellent lubrication and hydration, and is absorbed quickly without any stickiness or heaviness. This moisturizing emulsion of the present invention lubricates the skin and has excellent moisturizing effects.

[0233] Experimental Example 3

[0234] This invention utilizes membrane separation technology to separate and purify Dendrobium polysaccharides of different molecular weights from Dendrobium extract, yielding four samples: 10w–50wDa, 1w–10wDa, 1000Da–1wDa, and whole-fraction (not separated). These correspond to the Dendrobium polysaccharide extracts obtained in step (c) of humectant A, step (c) of humectant D, step (c) of humectant E, and the filtrate obtained in step (b) of humectant A, respectively. Through AQP3 gene expression efficacy testing and skin sensitivity evaluation, the optimal Dendrobium polysaccharide molecular fragments in terms of efficacy and skin sensitivity are selectively identified.

[0235] 1. AQP3 gene expression test

[0236] This experiment first used the MTT assay to detect cell viability and screen the maximum safe concentration for cell sample administration. The expression levels of each gene were then detected by quantitative real-time PCR. The test groupings and results are shown in Tables 8-9. The sample preparation method included adding 0.00781 wt% of each Dendrobium polysaccharide extract to the culture medium and heating and stirring at 55-60°C for 10-15 min.

[0237] Table 8 AQP3 Test Grouping

[0238]

[0239] Table 9. Test results of AQP3 in Dendrobium polysaccharides of different molecular weights

[0240]

[0241]

[0242] The test results above show that Dendrobium polysaccharides with molecular weights of 10w-50wDa and 1w-10wDa can promote AQP3 gene expression, with Dendrobium polysaccharides with molecular weights of 10w-50wDa showing better effects.

[0243] 2. Skin feel test

[0244] Different molecular weight Dendrobium polysaccharide extracts: 10w–50wDa (Sample 1), 1w–10wDa (Sample 2), 1000Da–1wDa (Sample 3), and the whole segment (Sample 4) were each prepared as 5wt% aqueous solutions. Eight testers applied the extracts and evaluated their smoothness and other skin feel, ranking them by preference. The specific preparation of the aqueous solutions involved adding each molecular weight Dendrobium polysaccharide extract at 5wt% to water and stirring for 10–15 minutes. The test results are shown in Table 10.

[0245] Table 10 Results of skin feel test for Dendrobium polysaccharides of different molecular weights

[0246] Trial user serial number Preference 1 1＞4＞2＞3 2 1＞4＞2＞3 3 1＞4＞2＞3 4 1＞4＞2＞3 5 1＞4＞2＞3 6 1＞4＞2＞3 7 1＞4＞2＞3 8 1＞4＞2＞3

[0247] The test results above show that all eight testers agreed that the 10w-50wDa Dendrobium polysaccharide had the best smoothness, followed by the whole-section sample, with significant differences between the samples. In conclusion, the 10w-50wDa Dendrobium polysaccharide has superior moisturizing effect and skin feel.

[0248] Experiment Example 4

[0249] Verification of supramolecular interactions

[0250] Rheological test:

[0251] Test sample:

[0252] 0.5% HA: i.e., 0.5 wt% sodium hyaluronate aqueous solution;

[0253] 0.5% Dendrobium polysaccharide: i.e., 0.5wt% of an aqueous solution of Dendrobium polysaccharide (lyophilized powder of Dendrobium polysaccharide extract obtained in step (c) of humectant A) with a molecular weight of 10w~50wDa;

[0254] 0.5% Dendrobium polysaccharide + HA solution mixed sample: i.e. 0.5wt% (sodium hyaluronate + Dendrobium polysaccharide with a molecular weight of 10w~50wDa) aqueous solution (the mass ratio of Dendrobium polysaccharide to HA is 5:2, and Dendrobium polysaccharide is the lyophilized powder of Dendrobium polysaccharide extract obtained in step (c) as humectant A);

[0255] 0.5% Dendrobium polysaccharide-HA supramolecular polysaccharide assembly sample: The lyophilized powder of 10w~50wDa Dendrobium polysaccharide extract obtained in step (c) of humectant A was compounded with HA at a mass ratio of 5:2 and dissolved in water. After homogenization in a homogenizer (2500rpm, 10min), the mixture was lyophilized to obtain the Dendrobium polysaccharide-HA supramolecular polysaccharide assembly product. Then, the product was prepared into a 0.5wt% aqueous solution.

[0256] In the preparation of the above samples, they were dispersed and dissolved in distilled water at the corresponding concentration, heated and stirred in a water bath at 40°C for 1 hour, and then placed at room temperature and shaken in a shaker at room temperature for 4 hours.

[0257] Rheological tests were performed on the above samples to investigate the viscosity differences between different samples and to verify the existence of supramolecular interactions. The test results are shown in […]. Figure 2 .

[0258] The rheological test results show that both the Dendrobium polysaccharide solution and the HA solution have very low viscosity. However, the viscosity increases significantly when they are combined. The viscosity of the Dendrobium polysaccharide-HA supramolecular polysaccharide assembly solution is higher than that of HA or Dendrobium polysaccharide single solutions at the same concentration. Therefore, there is a synergistic effect between Dendrobium polysaccharide and HA molecules, which intensifies the polymerization of hydrogen bonds between polysaccharide molecules. This results in the viscosity of the Dendrobium polysaccharide-HA supramolecular system being higher than that of HA or Dendrobium polysaccharide single polysaccharide solutions at the same concentration, thus exhibiting a thickening effect. Solution mixing also has a thickening effect, but it is not as strong as that of supramolecular polysaccharides, indicating that the supramolecular polysaccharide assembly has strong interactions.

[0259] Enhanced efficacy of supramolecular combination

[0260] This study investigated the hygroscopic effects of Dendrobium polysaccharide, sodium hyaluronate, a mixture of Dendrobium polysaccharide and sodium hyaluronate solutions, and Dendrobium polysaccharide-HA supramolecular polysaccharide assembly samples under high humidity conditions through in vitro hygroscopic tests, thus verifying the efficacy of supramolecular samples.

[0261] Sample 1: Sodium hyaluronate;

[0262] Sample 2: Dendrobium polysaccharide with a molecular weight of 10w-50wDa (the Dendrobium polysaccharide extract obtained in step (c) of humectant A was dried);

[0263] Sample 3: Dendrobium polysaccharide + HA solution mixture (the freeze-dried powder of Dendrobium polysaccharide extract obtained in step (c) of humectant A was mixed with HA in solution at a mass ratio of 5:2 and then dried);

[0264] Sample 4: Dendrobium polysaccharide-HA supramolecular polysaccharide assembly (the freeze-dried powder of Dendrobium polysaccharide extract obtained in step (c) of humectant A was compounded with HA at a mass ratio of 5:2 and dissolved in water, homogenized in a homogenizer (2500 rpm, 10 min) and then dried).

[0265] The above samples (set up in triplicate) were dried to constant weight and then placed in a desiccator containing a saturated ammonium sulfate solution (theoretical relative humidity 85%). Their mass was measured and recorded at set time points of 0, 2, 4, and 8 hours. The moisture absorption rate of the samples was determined based on their mass before and after drying. The test results are shown below. Figure 3 .from Figure 3It can be seen that within 8 hours, the moisture absorption effect of sample 4 is significantly better than that of samples 1, 2 and 3, that is, the supramolecular samples of Dendrobium polysaccharide and sodium hyaluronate have a synergistic effect.

[0266] The test results above show that the moisturizer used in this invention has a synergistic effect when Dendrobium polysaccharide and sodium hyaluronate are assembled through supramolecular polysaccharide. While precisely promoting the high expression of AQP3, it forms a "DHA network" to achieve water capture, slow release and deep penetration.

[0267] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant-based moisturizing emulsion, characterized in that, Includes the following components by weight: The solvent consists of 70-85 parts, humectant 1-10 parts, emollient 5-20 parts, emulsifier 2-10 parts, thickener 0.1-1 parts, and chelating agent 0.01-0.1 parts. The moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (1-5):(1-5); the moisturizer also comprises sodium hyaluronate. The preparation of the moisturizer includes: (a) Using water as the extraction solvent, the stems of Dendrobium officinale were extracted, decolorized and deodorized, and filtered at a material-to-liquid ratio of 1:(10-80). The extract was separated by membrane separation and Dendrobium officinale polysaccharide extract with a molecular weight between 10w and 50wDa was collected. (b) The Dendrobium polysaccharide extract and water were used to extract Hydrangea macrophylla at a material-to-liquid ratio of 1:(10-100), and the extract was then decolorized, deodorized, and filtered; the Hydrangea macrophylla was subjected to high-temperature micro-pressure treatment before extraction. Alternatively, the preparation of the humectant includes: extracting Dendrobium officinale stems and Hydrangea macrophylla separately using water as the extraction solvent at a material-to-liquid ratio of 1:(10~100); decolorizing and deodorizing the extracts and filtering them; during the extraction of Dendrobium officinale stems, the process further includes: filtering the obtained filtrate using 10wDa and 50wDa membranes to collect Dendrobium officinale polysaccharide extracts with molecular weights between 10w and 50wDa; subjecting Hydrangea macrophylla to high-temperature micro-pressure treatment before extraction; and then mixing and compounding the Dendrobium officinale stem extract and Hydrangea macrophylla extract.

2. The plant-based moisturizing emulsion according to claim 1, characterized in that, The moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (3.5–4.5):(3–4).

3. The plant-based moisturizing emulsion according to claim 2, characterized in that, The moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of 4:3.

5.

4. The plant-based moisturizing emulsion according to any one of claims 1 to 3, characterized in that, The content of sodium hyaluronate in the moisturizer is 0.05wt% to 0.5wt%.

5. The plant-based moisturizing emulsion according to claim 4, characterized in that, The content of sodium hyaluronate in the moisturizer is 0.1wt% to 0.2wt%.

6. The plant-based moisturizing emulsion according to claim 1, characterized in that, In the preparation of Dendrobium stem extract and / or Hydrangea extract, the extraction temperature is 40–90°C; the extraction time is 1–4 h.

7. The plant-based moisturizing emulsion according to claim 1, characterized in that, The preparation of the moisturizer further includes: mixing the mixture of the Dendrobium stem extract and the Hydrangea macrophylla extract with sodium hyaluronate in a certain proportion, and then subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment; Alternatively, the Dendrobium stem extract can be mixed with sodium hyaluronate in a certain proportion, homogenized, microwaved, ultrasonicated and / or micro-pressurized, and then mixed with the Hydrangea macrophylla extract.

8. The plant-based moisturizing emulsion according to claim 1, characterized in that, It has at least one of the following characteristics: (1) The solvent includes water; (2) The chelating agent includes disodium EDTA; (3) It also includes 0.1 to 1 part by weight of preservative; the preservative includes any one or more of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, methylparaben, benzyl alcohol, benzoic acid, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea and iodopropynyl butylcarbamate.

9. The plant-based moisturizing emulsion according to claim 1, characterized in that, It has at least one of the following characteristics: (1) The emollients include any one or more of hydrogenated polyisobutylene, polydimethylsiloxane, polydimethylsiloxane alcohol, squalane, glycerides, jojoba esters, tocopherol, phytosterols, macadamia oleate, avocado extract and tocopheryl acetate. (2) The emulsifier includes any one or more of behenol, sucrose stearate, cetyl alcohol, cocoyl glucoside, glyceryl stearate, PEG-100 stearate, sodium stearoyl lactylate, hydrogenated lecithin, cetearyl alcohol and polyglycerol-3-methylglucose diisostearate; (3) The thickener includes any one or more of xanthan gum, high molecular weight cellulose, gum arabic and acrylic (ester) compounds.

10. The plant-based moisturizing emulsion according to claim 9, characterized in that, The glycerides include caprylic / capric triglycerides.

11. The plant-based moisturizing emulsion according to claim 9, characterized in that, The acrylic (ester) thickeners include any one or more of acrylic (ester) / C10-30 alkanol acrylate crosspolymers, carbomer, and sodium acrylate / sodium acryloyldimethyl taurate copolymers.

12. The method for preparing the plant-based moisturizing emulsion according to any one of claims 1 to 11, characterized in that, Includes the following steps: (1) Mix the humectant, thickener, and chelating agent in a solvent and heat to 80-82°C, then keep warm. (2) Mix the emollient and emulsifier and heat to 80-82°C. Stir and melt into a liquid. Then add the mixture to the material obtained in step (1) to obtain a mixture. (3) Homogenize the mixture until it is uniformly emulsified, defoam, and then cool it down; When the temperature drops to 70-75℃, homogenize until uniform; then cool to 60-65℃, keep warm and stir to obtain plant moisturizing emulsion.

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