Use of a cual double metal hydroxide

Abstract

The application relates to a double-metal hydroxide, in particular to application of a CuAl double-metal hydroxide, in particular to application of the CuAl double-metal hydroxide in preparation of a preparation for inactivation of pathogenic bacteria, fungi and viruses. The CuAl double-metal hydroxide is prepared by a formamide liquid phase stripping method. Compared with the prior art, the application solves the problem of high cost of a silver ion loaded layered double-metal hydroxide in the prior art, and realizes preparation of a low-cost, high-performance and green antibacterial material with a bactericidal and antiviral effect.

Description

Technical Field

[0001] This invention relates to a bimetallic hydroxide, specifically to the application of a CuAl bimetallic hydroxide. Background Technology

[0002] Many viruses, including Ebola and coronaviruses, are highly contagious and pathogenic. Furthermore, their rapid mutation rate makes vaccine development difficult to keep pace with viral mutations, posing a significant threat to human development. Physical isolation methods such as wearing masks and maintaining social distancing cannot kill the virus.

[0003] The emergence and spread of drug-resistant pathogens that have acquired new resistance mechanisms and led to antimicrobial resistance continue to threaten our ability to treat common infections with antibiotics. Of particular concern is the rapid global spread of multidrug-resistant and pan-drug-resistant bacteria (also known as "superbugs"), resulting in a large proportion of infections that cannot be treated with existing antimicrobial agents such as antibiotics.

[0004] With the continuous emergence of new viruses, viral variants, and drug-resistant bacteria and fungi, the key to preventing their spread lies in stopping the transmission of viruses, bacteria, and fungi among the population and promptly eliminating pathogens in the environment. However, physical disinfection methods such as heating or ultraviolet light require specialized equipment and energy supplies, and these methods can also cause harm to the human body, such as burns or eye damage. The large-scale use of chemical disinfectants can easily produce toxic substances, damage the environment, and pose serious threats to the health of animals and plants.

[0005] As is well known, silver ions have certain bactericidal and disinfecting effects. With the development of technology, loading silver ions onto novel materials—layered bimetallic hydroxides—has become one of the hot topics in the application of silver ion sterilization and disinfection, as described in Chinese patent CN202210504918.4. However, silver ions are a precious metal with a high price, and using them for sterilization and disinfection significantly increases costs and can lead to the loss of precious metals. Therefore, it is necessary to find a suitable new material to replace layered bimetallic hydroxides loaded with silver ions in order to reduce costs and minimize the loss of precious metals. Summary of the Invention

[0006] The purpose of this invention is to provide an application of CuAl bimetallic hydroxide to solve at least one of the above-mentioned problems, thereby addressing the high cost of layered bimetallic hydroxides loaded with silver ions used for sterilization and disinfection in the prior art, and achieving low-cost, high-performance inactivation of pathogenic microorganisms.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] The application of a CuAl bimetallic hydroxide in the preparation of formulations for the inactivation of pathogenic microorganisms (including pathogenic bacteria, fungi, and viruses), particularly for the inactivation of SARS-CoV-2 virus. The formulation may be a liquid preparation and can be applied by spraying or smearing.

[0009] Preferably, the CuAl bimetallic hydroxide is prepared by formamide liquid-phase exfoliation.

[0010] Preferably, the preparation method of the CuAl bimetallic hydroxide includes the following steps:

[0011] S1: Dissolve the copper source and aluminum source in deionized water to form a precursor mixture;

[0012] S2: Add the precursor mixture obtained in step S1 to the formamide solution and stir at low temperature under an inert gas atmosphere to obtain the reaction stock solution;

[0013] S3: Add sodium hydroxide solution to the reaction stock solution obtained in step S2 to adjust the pH to be alkaline. After centrifugation and washing, CuAl bimetallic hydroxide is obtained.

[0014] Preferably, the copper source is copper nitrate trihydrate, the aluminum source is aluminum nitrate nonahydrate, and the mass ratio of the copper source to the aluminum source is 90-500:40-250.

[0015] Preferably, the formamide solution has a volume fraction of 15-33%, and the volume ratio of the precursor mixture to the formamide solution is 1:1; the inert gas atmosphere is a nitrogen atmosphere, and the low-temperature stirring temperature is -5 to 5°C for 10-30 minutes.

[0016] Preferably, the concentration of the sodium hydroxide solution is 0.5-4M, and the pH is adjusted to 9-12; the centrifugation speed is 6000-8000 r / min.

[0017] Preferably, the CuAl bimetallic hydroxide is prepared as a storage solution for storage, and the storage solution is diluted into a working solution for use.

[0018] Preferably, the solvent for both the storage solution and the working solution is DMEM culture medium.

[0019] Preferably, the CuAl bimetallic hydroxide is dissolved in DMEM medium to prepare a 1 mg / mL stock solution.

[0020] Preferably, the CuAl bimetallic hydroxide is dissolved in DMEM medium and diluted to a working solution of 0.016-50 μg / mL.

[0021] The working principle of this invention is as follows:

[0022] The mechanism of action of CuAl hydroxide stems from the fact that Cu, among inorganic bactericides, possesses good antibacterial activity. Its antibacterial mechanism is generally believed to involve the gradual dissolution of Cu during the action of the antibacterial agent. 2+ Metal ions react with sulfhydryl and amino groups (containing S and N functional groups) in proteins and nucleic acids, reducing protein or enzyme activity and inhibiting bacterial metabolism and reproduction. The Al-O structure has been reported to have antibacterial effects, with Al-(OH) and Al-(OH)2 at its edge sites exhibiting strong antibacterial activity. Simultaneously, under light conditions, the surface of layered bimetallic hydroxides contains numerous hydroxyl groups. The hydroxide ions released in an aqueous environment generate a large number of reactive oxygen species (such as hydroxyl radicals ·OH) under light, damaging the bacterial cell membrane and thus disrupting the physiological structure of microorganisms, affecting cell growth and metabolism.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) CuAl hydroxide was prepared in one step by low-temperature formamide coprecipitation. The obtained CuAl hydroxide has a hexagonal nanosheet structure. The synthesis method is simple, easy to operate, mild, and the target product has high purity. It is safe and non-toxic and can be synthesized in large quantities at low cost.

[0025] (2) CuAl hydroxide material was used to inactivate pathogenic microorganisms, and the results showed that it had excellent virus killing and antibacterial effects. In the application of virus inactivation, under natural light and ultraviolet conditions, the CuAl hydroxide treatment group could significantly inhibit the infection of Vero-E6 cells by SARS-CoV-2 virus. In the antibacterial activity experiment, CuAl hydroxide showed extremely strong antibacterial activity.

[0026] (3) All reagents used in the preparation process are commercial products and do not require further processing.

[0027] (4) The synthesis method is simple. The antiviral and antibacterial effects of CuAl hydroxide do not depend on the role of the precious metal Ag, which can greatly reduce the preparation cost and the obtained material is easy to apply on a large scale.

[0028] This invention fully utilizes the structural adjustability of bimetallic hydroxides by simultaneously introducing Cu and Al elements into the bimetallic hydroxide layer. During the interaction with viruses and bacteria, the antiviral and antibacterial properties of CuAl hydroxides are further enhanced through the synergistic effect of Cu and Al elements. Attached Figure Description

[0029] Figure 1The images show the CuAl hydroxide prepared in Example 1 and the CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1.

[0030] Figure 2 The X-ray diffraction patterns of CuAl hydroxide prepared in Example 1 and CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1 are shown below.

[0031] Figure 3 The X-ray photoelectron spectra of CuAl hydroxide prepared in Example 1 and CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1 are shown. In the figures, a and c are the X-ray photoelectron spectra of Cu in the products of Example 1 and Comparative Example 1, respectively; b and d are the X-ray photoelectron spectra of Al in the products of Example 1 and Comparative Example 1, respectively; and e is the X-ray photoelectron spectra of Ag in the product of Comparative Example 1.

[0032] Figure 4 The images shown are transmission electron microscope (TEM) images of the CuAl hydroxide prepared in Example 1, where a is a TEM image of the product of Example 1, and b and c are the material and the distribution of Cu, Al and O on the material surface, respectively.

[0033] Figure 5 The image shows a transmission electron microscope (TEM) image of CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1. In the image, a is the TEM image of the product of Comparative Example 1, and b, c, and d are the material and the distribution of Cu, Al, O, and Ag on the material surface, respectively.

[0034] Figure 6 Fourier transform-infrared absorption spectra of CuAl hydroxide prepared in Example 1 and CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1.

[0035] Figure 7 The UV-Vis absorption spectra of CuAl hydroxide prepared in Example 1 and CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1 are shown.

[0036] Figure 8 The virus-killing effects of CuAl hydroxide prepared in Example 1 and CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1 are shown, where a represents the virus-killing effect of the product in Example 1 and b represents the virus-killing effect of the product in Comparative Example 1. Detailed Implementation

[0037] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0038] Unless otherwise specified, the reagents used in the following examples are commercially available products that are routinely available to those skilled in the art, and the methods employed are well-known in the art.

[0039] Example 1

[0040] Preparation of CuAl hydroxide:

[0041] Dissolve 181.2 mg of copper nitrate trihydrate and 94 mg of aluminum nitrate nonahydrate in 20 mL of deionized water. Add the mixture dropwise to a three-necked flask containing 20 mL of 23% formamide solution, then transfer to a cryogenic reaction vessel and maintain the temperature at 0°C. Purge with nitrogen and stir for 15 minutes. Slowly add a pre-prepared 2.5 M sodium hydroxide solution dropwise to the three-necked flask to maintain the pH at 10. Maintain the cryogenic nitrogen atmosphere; the reaction should complete within 15 minutes. Collect the product by centrifugation, wash repeatedly with deionized water and anhydrous ethanol, and then keep it moist for later use.

[0042] Comparative Example 1

[0043] Preparation of CuAl hydroxide loaded with Ag nanoparticles:

[0044] The preparation method is basically the same as in Example 1, except that 1 mg of silver nitrate is added to each of the mixed solutions of copper nitrate trihydrate and aluminum nitrate nonahydrate.

[0045] Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Inactivation Test:

[0046] Virus inactivation: CuAl hydroxide and CuAl hydroxide powder loaded with Ag nanoparticles were dissolved in DMEM medium to prepare a 1 mg / mL stock solution. During the experiment, the CuAl hydroxide and Ag nanoparticle-loaded CuAl hydroxide powder stock solution was diluted in DMEM medium to final concentrations of 50 μg / mL, 10 μg / mL, 2 μg / mL, 0.4 μg / mL, 0.08 μg / mL, and 0.016 μg / mL, respectively, into working solutions. SARS-CoV-2 (10 5 PFU was mixed with different concentrations of working solution and incubated at room temperature for 0.5 hours. Each compound was divided into two groups: one group was incubated in the dark, and the other group was incubated under natural light. Then, the mixture was added to Vero-E6 cells. After 24 hours, the infection rate of SARS-CoV-2 in each group was detected by immunofluorescence.

[0047] The method for evaluating the virus-killing effect of the above-mentioned CuAl hydroxide and CuAl hydroxide loaded with Ag nanoparticles is as follows: the virus-killing effect of the materials is evaluated by detecting the infection rate of SARS-CoV-2 virus in Vero-E6 cells by immunofluorescence.

[0048] Specific experimental steps:

[0049] A 1 mg / mL stock solution was prepared by dissolving CuAl hydroxide and CuAl hydroxide powder loaded with Ag nanoparticles in DMEM medium.

[0050] Twelve hours in advance, seed Vero-E6 cells into 24-well plates to ensure a cell density of 80%–90% during viral infection.

[0051] During the experiment, CuAl hydroxide and CuAl hydroxide stock solution loaded with Ag nanoparticles were diluted with DMEM medium to make working solutions with final concentrations of 50 μg / mL, 10 μg / mL, 2 μg / mL, 0.4 μg / mL, 0.08 μg / mL and 0.016 μg / mL, respectively.

[0052] SARS-CoV-2(10 5 PFU was mixed with different concentrations of compounds and incubated at room temperature for 0.5 hours. CuAl hydroxide and CuAl hydroxide loaded with Ag nanoparticles were each divided into two groups, one group was incubated under dark conditions and the other group was incubated under natural light.

[0053] Take 100 μL of virus from each treatment group, mix it with CuAl hydroxide (Example 1) and CuAl hydroxide loaded with Ag nanoparticles (Comparative Example 1), add it to the prepared Vero-E6 cells, mix gently, and incubate in an incubator at 37°C and 5% CO2.

[0054] After 2 hours, discard the supernatant and add fresh DMEM culture medium.

[0055] 24 hours after viral infection, immunofluorescence experiments were performed using SARS-CoV-2N protein antibodies to detect the SARS-CoV-2 virus infection rate in each group. The half-maximal inhibitory concentration (EC50) of different materials against SARS-CoV-2 was calculated to evaluate the inhibitory effects of CuAl hydroxide and CuAl hydroxide loaded with Ag nanoparticles on SARS-CoV-2 infection.

[0056] Experimental results are as follows Figure 8Under natural light conditions, the EC50 of CuAl hydroxide material loaded with Ag nanoparticles against SARS-CoV-2 was 2.153 μg / mL, which was higher than the EC50 of CuAl hydroxide material without Ag nanoparticles (1.056 μg / mL). Therefore, CuAl hydroxide has a better anti-SARS-CoV-2 effect than CuAl hydroxide loaded with Ag nanoparticles.

[0057] Antifungal activity assay:

[0058] To evaluate the antifungal activity of the new material, we determined its minimum inhibitory concentrations (MICs) in vitro using the Candida albicans SC5314 strain. These yeast cells were cultured on yeast extract peptone glucose medium (YPD) overnight at 30°C to obtain single colonies. These single colonies were resuspended in YPD broth to a turbidity of 1.0 × 10⁻⁶. 8 cells / mL. MICs were determined by microdilution assays according to the large-dilution reference method of the National Clinical Laboratory Standards Board (NCCLSB).

[0059] Candida albicans strain SC5314 (100 μL, 5.0 × 10⁻⁶ ...) was added. 4 Yeast cells (200 μL / mL) were added to wells in rows A and B of a 96-well plate. Row A contained 200 μL of yeast cells and the antimicrobial material for testing, while row B contained only 200 μL of yeast cells. 200 μL of RPMI 1640 medium (Gibco, Langley, OK) was added to wells in row C as a blank control, and 200 μL of RPMI 1640 medium and the antimicrobial material were added to wells in row D. The antimicrobial material (titanium-based compound) was dispersed in phosphate-buffered saline (PBS) and serially diluted twofold in RPMI 1640 medium to a final concentration range of 0.390–200 μg / mL. Fluconazole (FLC) was used as a positive control, with a final concentration range of 0.125–64 μg / mL.

[0060] The fungal growth was determined by measuring the optical density at a wavelength of 620 nm after incubation at 30℃ for 24 hours without shaking. The experimental results are shown in Table 1. It can be seen that the antifungal activity of CuAl hydroxide is similar to that of CuAl hydroxide loaded with Ag nanoparticles. Therefore, the antifungal activity of this material does not depend on the loading of the noble metal Ag.

[0061] Table 1 Results of antifungal activity assay

[0062] name MIC (μg / mL) CuAl hydroxide 8 CuAl hydroxide loaded with Ag nanoparticles 8

[0063] Figure 1These are real digital photos of CuAl hydroxide and CuAl hydroxide material loaded with Ag nanoparticles prepared in Example 1 and Comparative Example 1. It can be seen that both materials have the Tyndall effect, which indicates that the materials have an ultrathin structure.

[0064] Figure 2 The X-ray diffraction patterns of CuAl hydroxide and CuAl hydroxide material loaded with Ag nanoparticles prepared in Example 1 and Comparative Example 1 are obtained at a scanning speed of 4° / min and a scanning range of 5-75°. Both are close to the standard card (PDF#37-0630) of carbonate-intercalated copper-aluminum bimetallic hydroxide. Slight peak shifts and differences may be due to interlayer spacing variations caused by nitrate intercalation, consistent with the diffraction peak characteristics of layered bimetallic hydroxides.

[0065] Figure 3 Examples 1 and 2 are CuAl hydroxide and CuAl hydroxide materials loaded with Ag nanoparticles prepared in Comparative Example 1. (a, c)Cu, (b, d)Al and (e)Ag are X-ray photoelectron spectra of the corresponding elements. It can be seen that: 1) Cu in CuAl hydroxide mainly exhibits a divalent state. Compared with before Ag doping, the valence state of Cu decreases after loading, and Cu with 0 or 1 valence states appears. 2) The Al element does not change much before and after loading. 3) Ag element is successfully loaded.

[0066] Figure 4 The images show transmission electron microscopy (TEM) images of the CuAl hydroxide material prepared in Example 1 (a), which show small hexagonal nanosheets and large rod-shaped morphologies; and transmission electron microscopy (TEM) images and energy dispersive spectroscopy (EDS) elemental distribution maps of the CuAl hydroxide material (b) and (c), which show that Cu, Al, and O are uniformly distributed on the surface.

[0067] Figure 5 The images show: (a) Transmission electron microscopy (TEM) images of CuAl hydroxide loaded with Ag nanoparticles prepared in Comparative Example 1, exhibiting sheet-like, mesh-like, and granular morphologies; (b) TEM images and energy-dispersive X-ray spectroscopy (EDS) elemental distribution diagrams of CuAl hydroxide loaded with Ag nanoparticles, showing uniform distribution of Cu, Al, and O on the nanosheet surface; (c) TEM images and EDS elemental distribution diagrams of CuAl hydroxide loaded with Ag nanoparticles, showing only Cu distribution in the mesh-like structure, potentially indicating the formation of new Cu-based compound structures; and (d) TEM images and EDS elemental distribution diagrams of CuAl hydroxide loaded with Ag nanoparticles, showing Ag loaded on CuAl hydroxide in the form of nanoparticles.

[0068] Figure 6These are the Fourier transform-infrared spectra of CuAl hydroxide and CuAl hydroxide materials loaded with Ag nanoparticles prepared in Example 1 and Comparative Example 1. It can be seen that the layered bimetallic hydroxides before and after Ag loading both have a wavelength at 3480 cm⁻¹. -1 1630cm -1 1380cm -1 Infrared transmission peaks appeared at the locations, which were attributed to the OH, HOH, and NO3 between the LDH layers of water, respectively. - The MO transmission peak is consistent with the classic LDH structure;

[0069] Figure 7 The images show the UV-Vis absorption spectra of CuAl hydroxide and CuAl hydroxide material loaded with Ag nanoparticles prepared in Example 1 and Comparative Example 1. After loading Ag, the sample is easily discolored by light and its properties change. It can be seen that the light absorption performance decreases after loading Ag.

[0070] Figure 8 This study tested the inactivation activity against Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2). The infection rate of SARS-CoV-2 virus in Vero-E6 cells was detected by immunofluorescence to evaluate the virus-killing effect of the material. Under natural light conditions, the EC50 of the Ag-loaded CuAl hydroxide material against SARS-CoV-2 was 2.153 μg / mL, which was higher than the EC50 of the unloaded CuAl hydroxide material (1.056 μg / mL). Therefore, CuAl hydroxide exhibited superior anti-SARS-CoV-2 efficacy compared to Ag-loaded CuAl hydroxide.

[0071] Table 2 shows the elemental contents of CuAl hydroxide and CuAl hydroxide materials loaded with Ag nanoparticles prepared in Example 1 and Comparative Example 1, as measured by inductively coupled plasma mass spectrometry.

[0072] Table 2 Results of elemental content determination

[0073] Cu (mg / mL) Al (mg / mL) Ag (mg / mL) CuAl hydroxide 1.64 0.260 / CuAl hydroxide loaded with Ag nanoparticles 3.32 0.48 0.096

[0074] Example 2

[0075] Preparation of CuAl hydroxide materials:

[0076] Dissolve 362.4 mg of copper nitrate trihydrate and 188 mg of aluminum nitrate nonahydrate in 40 mL of deionized water. Then, add the mixture dropwise to a three-necked flask containing 40 mL of 23% (v / v) formamide solution. Transfer the flask to a cryogenic reaction vessel, maintain the temperature at 0°C, and then purge with nitrogen and stir for 10 minutes. Finally, slowly add the prepared 2.5 M sodium hydroxide solution dropwise to the flask to maintain the pH at 10. Maintain the cryogenic nitrogen environment; the reaction should complete within 15 minutes. Collect the product by centrifugation, wash repeatedly with deionized water and anhydrous ethanol, and then keep it moist for later use.

[0077] The CuAl hydroxide material prepared in this embodiment showed similar performance in the inactivation and antifungal activity tests for Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) as in Example 1.

[0078] Example 3

[0079] Preparation of CuAl hydroxide materials:

[0080] Dissolve 90.6 mg of copper nitrate trihydrate and 48 mg of aluminum nitrate nonahydrate in 10 mL of deionized water. Then, add the mixture dropwise to a three-necked flask containing 10 mL of 23% (v / v) formamide solution. Transfer the flask to a cryogenic reaction vessel, maintain the temperature at 0°C, and then purge with nitrogen and stir for 10 minutes. Finally, slowly add the prepared 0.5 M sodium hydroxide solution dropwise to the flask to maintain the pH at 10. Maintain the cryogenic nitrogen environment; the reaction should complete within 15 minutes. Collect the product by centrifugation, wash repeatedly with deionized water and anhydrous ethanol, and then keep it moist for later use.

[0081] The CuAl hydroxide material prepared in this embodiment showed similar performance in the inactivation of SARS-CoV-2 and antifungal activity tests as in Example 1.

[0082] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. The application of a CuAl bimetallic hydroxide in the preparation of formulations for inactivating SARS-CoV-2 virus; The CuAl bimetallic hydroxide was prepared by a formamide liquid-phase exfoliation method, comprising the following steps: S1: Dissolve the copper source and aluminum source in deionized water to form a precursor mixture; S2: Add the precursor mixture obtained in step S1 to the formamide solution and stir at low temperature under an inert gas atmosphere to obtain the reaction stock solution; S3: Add sodium hydroxide solution to the reaction stock solution obtained in step S2 to adjust the pH to be alkaline, and obtain CuAl bimetallic hydroxide after centrifugation and washing; The CuAl bimetallic hydroxide described herein has hexagonal nanosheet and rod-shaped morphologies.

2. The application of the CuAl bimetallic hydroxide according to claim 1, characterized in that, The copper source is copper nitrate trihydrate, and the aluminum source is aluminum nitrate nonahydrate. The mass ratio of the copper source to the aluminum source is 90-500:40-250.

3. The application of the CuAl bimetallic hydroxide according to claim 1, characterized in that, The formamide solution has a volume fraction of 15-33%, and the volume ratio of the precursor mixture to the formamide solution is 1:1; the inert gas atmosphere is a nitrogen atmosphere, and the low-temperature stirring temperature is -5 to 5℃ for 10-30 min.

4. The application of the CuAl bimetallic hydroxide according to claim 1, characterized in that, The concentration of the sodium hydroxide solution is 0.5-4M, and the pH is adjusted to 9-12; the centrifugation speed is 6000-8000 r / min.

5. The application of a CuAl bimetallic hydroxide according to any one of claims 1-4, characterized in that, The CuAl bimetallic hydroxide is prepared as a storage solution for storage, and the storage solution is diluted to form a working solution for use.

6. The application of the CuAl bimetallic hydroxide according to claim 5, characterized in that, The solvent for both the storage solution and the working solution is DMEM culture medium.

7. The application of the CuAl bimetallic hydroxide according to claim 6, characterized in that, The CuAl bimetallic hydroxide was dissolved in DMEM medium to prepare a 1 mg / mL stock solution.

8. The application of the CuAl bimetallic hydroxide according to claim 6, characterized in that, The CuAl bimetallic hydroxide was dissolved in DMEM medium and diluted to a working solution of 0.016-50 μg / mL.

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