Use of curcumin in regulating plant chlorophyll content

Abstract

The present invention discloses the use of curcumin in regulating the chlorophyll content of plants, belonging to the technical field of plant growth regulators. Specifically, curcumin can regulate the growth of plants and at the same time can also regulate the chlorophyll content in plants. The research of the present invention finds that curcumin can both promote and inhibit seed germination, and it mainly inhibits or promotes seed germination by regulating the levels of endogenous hormones in plants; in addition, this growth regulator can also enhance or weaken the photosynthesis of leaves by regulating the degradation and synthesis of chlorophyll in plants, and regulate energy metabolism so as to control the plant height and flowering of plant seedlings.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and particularly relates to the use of curcumin in regulating the chlorophyll content of plants. Background Art

[0002] The long-term use of chemical pesticides has brought various drawbacks, such as the generation of pest resistance, residual toxicity, and environmental pollution. At present, many highly toxic, highly residual, and persistent pesticides have been banned. An ideal pesticide should be highly effective against pests, safe for non-target organisms, easily decomposable itself, and the decomposition products should be harmless to the environment. Plants are a natural treasure house of bioactive compounds. It is estimated that plants produce more than 400,000 secondary metabolites. Terpenoids, alkaloids, flavonoids, steroids, phenols, unique amino acids, and polysaccharides in these metabolites all have insecticidal or antibacterial activities. Exploring new active lead compounds or new action targets from plants and developing new pesticides through analog synthesis or bio-rational design have become the hotspots in current pesticide chemistry and pesticide toxicology research...

[0003] In the late 1950s, it was first reported that wheat and corn contain 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one, abbreviated as "DIMBOA". As a methoxylated hydroxamic acid, DIMBOA exists in plants in the form of glycosides. When the plant is damaged, it can be converted into a more toxic aglycone under the action of glycosidase. Xu Guofeng et al. reported that when the concentration is 1.0 mg / ml, the spore germination inhibition rate of DIMBOA against Puccinia striiformis f. sp. tritici can reach 79.31%.

[0004] Curcuma longa L. is a plant of the Zingiberaceae family and is a traditional Chinese medicine. Curcumin (Cur) is a polyphenolic compound extracted from the rhizomes of Curcuma longa L. It has attracted much attention because of its various biological activities such as antioxidant, anti-inflammatory, anti-apoptotic, and immunomodulatory effects. Cur is a multifunctional molecule that can effectively penetrate the blood-brain barrier and neuronal membranes, regulate neurotransmitter levels, cell signaling, and brain function, inhibit mitochondrial dysfunction, reduce oxidative stress, and decrease neurodegeneration, thereby improving neuronal and behavioral dysfunction. Curcumin also has good photosensitivity. In photodynamic therapy, because it is edible and has low toxicity and side effects, it has broad prospects in tumor treatment. Using the Chinese medicine monomer curcumin in combination with photodynamic therapy at a small dose and multiple frequencies can effectively kill cervical cancer tumor cells. In food preservation, an antioxidant carboxymethyl cellulose edible film incorporated with curcumin-loaded nanoparticles can effectively delay the oxidative deterioration of chilled meat and extend the shelf life. In addition, in recent years, the research on the application of curcumin in aquaculture has gradually increased. It has been found that curcumin can significantly improve the growth performance, digestive ability, and antioxidant ability of yellow catfish, and has a certain antibacterial effect on the main pathogenic bacteria in aquaculture such as Aeromonas hydrophila. Currently, there is no research on the application of curcumin in weed control. And currently, there is no research on the application of curcumin in plant growth regulation. Summary of the Invention

[0005] In view of the above deficiencies in the prior art, the present invention provides a use of curcumin in regulating the chlorophyll content of plants.

[0006] To achieve the above object, the technical solution adopted by the present invention to solve its technical problems is:

[0007] Use of curcumin in regulating the chlorophyll content of plants.

[0008] Further, the plant is a cereal or cruciferous plant.

[0009] Further, the cereal plants are rice and wheat, and the cruciferous plant is Shanghaiqing.

[0010] Use of curcumin in regulating seed germination.

[0011] Further, soak the plant seeds in a solution with a curcumin concentration of 1×10 1 ~1×10 -3 mg / L for 10 - 24 h; wherein, the curcumin concentration is based on the curcumin concentration.

[0012] Further, soak the plant seeds in a solution with a curcumin concentration of 2×10 -2 ~1×10 -3 mg / L; wherein, the curcumin concentration is based on the curcumin concentration.

[0013] The beneficial effects of adopting the above further technical solution are as follows: The solution containing curcumin within this concentration range can promote seed germination, thereby increasing the germination rate.

[0014] Use of curcumin in promoting plant growth and flowering during the seedling stage.

[0015] Furthermore, during the seedling stage of the plant, a solution with a curcumin concentration of 1×10 1 ~1×10 -3 mg / L is applied to the plant 3 to 5 times, and the application amount of the solution per plant each time is 0.2 to 0.5 L; among them, the curcumin concentration is based on the curcumin concentration.

[0016] Furthermore, during the seedling stage of the plant, a solution with a curcumin concentration of 2×10 -1 ~2×10 -3 mg / L is applied to the plant; among them, the curcumin concentration is based on the curcumin concentration.

[0017] The beneficial effects of adopting the above further technical solution are as follows: The solution containing curcumin within this concentration range can increase the chlorophyll content of plant leaves, promote the elongation of plant seedlings, and promote plant flowering.

[0018] A preparation for regulating the chlorophyll content of plants, including the above-mentioned curcumin and adjuvants.

[0019] Furthermore, the curcumin concentration is 2×10 -1 ~2×10 -3 mg / L.

[0020] Furthermore, the preparation is an aqueous solution, suspension, wettable powder or granule.

[0021] A growth regulator containing curcumin, the growth regulator includes curcumin, and the mass fraction of curcumin in the growth regulator is 20 - 36%; preferably, the mass fraction of curcumin in the growth regulator is 25%.

[0022] When the growth regulator is an aqueous solution, the growth regulator further includes a solvent with a mass fraction of 0.1%, a stabilizer with a mass fraction of 2%, an antifreeze with a mass fraction of 5%, and a preservative with a mass fraction of 0.1 - 0.5%; among them, the solvent is xylene, toluene or cyclohexanone, the stabilizer is triphenyl phosphite or epichlorohydrin, the antifreeze is ethylene glycol, propylene glycol, glycerol or urea, and the preservative is benzoic acid or sodium benzoate.

[0023] When the growth regulator is a suspension agent, the growth regulator further includes a dispersant with a mass fraction of 3%, a wetting agent of 5-7%, a thickening agent of 2%, a preservative of 0.1-0.5%, an antifoaming agent of 0.1-0.5% and an antifreezing agent of 4%; wherein, the dispersant is alkyl naphthalene sulfonate, polycarboxylate or lignin sulfonate, the wetting agent is alkylphenol polyoxyethylene phosphate, phenethylphenol polyoxyethylene phosphate, alkyl sulfate, alkyl sulfonate or naphthalene sulfonate, the thickening agent is xanthan gum, magnesium aluminum silicate or bentonite, the preservative is benzoic acid or sodium benzoate, the antifoaming agent is silicone-based, such as polydimethylsiloxane; the antifreezing agent is glycerol, urea, ethylene glycol or propylene glycol.

[0024] When the growth regulator is a wettable powder, the growth regulator further includes a dispersant with a mass fraction of 6%, a wetting agent of 3% and a filler of 4-5%; wherein, the dispersant is polycarboxylate, lignin sulfonate or alkyl naphthalene sulfonate, the wetting agent is alkyl sulfonate, alkyl sulfate or naphthalene sulfonate, and the filler is light calcium carbonate, talcum powder, diatomite, kaolin or attapulgite.

[0025] When the growth regulator is a granule, the growth regulator further includes a dispersant with a mass fraction of 4%, a wetting agent of 5%, a disintegrant of 5%, a binder of 0.3% and a filler of 28-37%; wherein, the dispersant is polycarboxylate, lignin sulfonate or alkyl naphthalene sulfonate, the wetting agent is alkyl sulfate, polyvinyl alcohol, alkyl sulfonate or naphthalene sulfonate, the disintegrant is citric acid, ammonium sulfate, glucose, urea or sodium bicarbonate, the binder is corn starch, microcrystalline cellulose or diatomite, and the filler is light calcium carbonate, talcum powder, diatomite, kaolin or attapulgite.

[0026] In addition, in practice, various auxiliaries in the growth regulators of the above different dosage forms (the auxiliaries are any several of emulsifiers, wetting agents, stabilizers, thickening agents, preservatives, antifreezing agents, dispersants, fillers, disintegrants and binders) can be used as the auxiliaries in the growth regulator in this embodiment as long as they can improve the physical and chemical properties of the medicament, enhance the efficacy and facilitate transportation and storage and other properties during the processing or use of the growth regulator.

[0027] Advantages of the present invention:

[0028] The growth regulator in the present invention can both promote and inhibit seed germination. It mainly realizes the inhibition or promotion of seed germination by regulating the level of plant endogenous hormones; in addition, the growth regulator can also control the plant height and flowering of plant seedlings by regulating the degradation and synthesis of chlorophyll in plants, enhancing or weakening the photosynthesis of leaves and regulating energy metabolism. Specific embodiments

[0029] The specific embodiments of the present invention will be described below to facilitate those skilled in the art of this technology to understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those of ordinary skill in the art of this technology, as long as various changes are within the spirit and scope of the present invention defined and determined by the appended claims, these changes are obvious, and all inventions and creations using the concept of the present invention are within the scope of protection.

[0030] Example 1: Effect of Curcumin on Seed Germination

[0031] Rice seeds (variety: Chuanyou 6203), wheat seeds (variety: Chuanmai 88), and Shanghaiqing seeds (variety: Siji Shanghaiqing) were taken.

[0032] Prepare the experimental solutions: Dilute hematoporphyrin monomethyl ether with a concentration of 80% to solutions of 1×10 2 、5×10 2 、1×10 3 、5×10 3 、5×10 4 、5×10 5 、1×10 6 times respectively with distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80. Use distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as the control group.

[0033] Conduct the seed soaking and germination test on rice, wheat, and Shanghaiqing seeds. Specifically, place 50 seeds in each petri dish, repeat each concentration 3 times. After culturing at 25°C for 72 hours, observe the germination of wheat and Shanghaiqing. After culturing under the same conditions for 120 hours, observe the germination of rice. Calculate the germination rate according to the following formula. The results are shown in Table 1. The germination rate calculation formula is as follows:

[0034]

[0035] Table 1: Effects of Different Concentrations of Curcumin on the Germination Rates of Rice, Wheat, and Shanghaiqing Seeds

[0036]

[0037]

[0038] Note: IBM SPSS Statistics 25 data processing software was used for statistical analysis, and Duncan's new multiple range method was used for significance analysis of differences. Different lowercase letters marked after the data in the same column indicate significant differences (P<0.05). The same applies hereinafter.

[0039] Table 1 shows that curcumin has a certain effect on the germination of rice, wheat, and Shanghaiqing seeds. When the concentration of the agent is 1×10 1It slightly inhibits the germination of rice seeds at mg / L, and the effect of promoting the germination of rice seeds is better when treated with the agent at a concentration of 2×10 -2 ~1×10 -3 mg / L; in the treatment of wheat with the agent, the germination rate reaches the highest when soaked in the agent at a concentration of 2×10 -2 mg / L; in the treatment of Shanghaiqing, the germination rate of seeds is the highest when the treatment concentration of the agent is 2×10 -3 mg / L. Generally speaking, there is no significant difference in the germination rate between all treatment groups of the agent on wheat and Shanghaiqing and their corresponding control groups. The agent concentration is 2×10 -2 ~1×10 -3 mg / L is the best for promoting the germination of the three crops.

[0040] Example 2 Effects of curcumin on plant growth traits

[0041] 1. Effects on chlorophyll content at the seedling stage of plants

[0042] Select healthy rice seedlings, wheat seedlings, and Shanghaiqing seedlings with similar growth conditions, transplant them into pots, 5 plants per pot, 10 pots per group, and repeat each group 3 times.

[0043] Prepare the same experimental solution as in Example 1, and use distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as the control group.

[0044] Spray each group of potted plants. Each spraying cycle is 5 days, and the single spraying amount per plant is 0.2L. 5 days after the 3rd spraying, use a SPAD-502 portable chlorophyll meter to measure the chlorophyll content, and record the SPAD value of each plant. The results are shown in Table 2.

[0045] Table 2 Effects of curcumin at different concentrations on the chlorophyll content of rice, wheat, and Shanghaiqing

[0046]

[0047]

[0048] It can be seen from Table 2 that curcumin has a certain effect on the change of chlorophyll content at the seedling stage of rice, wheat, and Shanghaiqing. It shows that when the agent concentration is relatively high, it has more or less inhibitory effects on the chlorophyll content of the three crops. For rice, the chlorophyll content of the rice treatment group at a concentration of 2×10 -1 ~2×10 -3 mg / L is significantly higher than that of the control group and other concentration treatment groups; for the effect on wheat, there is no significant difference in the chlorophyll content between the treatment groups of different concentrations of the agent on wheat and the control group, but the chlorophyll content is the lowest at the highest concentration; for Shanghaiqing, the agent concentration is 2×10 -1 ~2×10-3 The chlorophyll content in the leaves of the mg / L treatment group was significantly higher than that of the control group; overall, under the action of the agent at a concentration of 2×10 -2 mg / L, the chlorophyll content in the leaves of the three crops reached the maximum value.

[0049] 2. Effects on plant seedling length

[0050] Select healthy rice seedlings, wheat seedlings, and Shanghai green vegetable seedlings with similar growth conditions, transplant them into pots, 5 plants per pot, 10 pots per group, and repeat each group 3 times.

[0051] Prepare the same experimental solution as in Example 1, and use distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as the control group.

[0052] Spray each group of potted plants. Each spraying cycle is 5 days, and the single spraying amount per plant is 0.2L. Record the seedling length of each plant 5 days after the 3rd spraying, and the results are shown in Table 3.

[0053] Table 3 Effects of curcumin at different concentrations on the seedling length of rice, wheat, and Shanghai green vegetables

[0054]

[0055]

[0056] Table 3 shows that curcumin had a certain effect on the seedling length of rice, wheat, and Shanghai green vegetables. For rice and Shanghai green vegetables, there was no significant difference in the seedling length between the treatment groups with different concentrations of the agent and the control group. The seedling length of the crops in the treatment group with a concentration of 2×10 -2 mg / L was the largest compared to other treatments; for wheat, the seedling length of the treatment group with a concentration of 2×10 -1 ~2×10 -3 mg / L was significantly higher than that of the control group, and the seedling length value was also the largest when the concentration reached 2×10 -2 mg / L; overall, the highest concentration of the agent had a certain inhibitory effect on the elongation of the seedling stage of the three crops, and when the agent concentration reached 2×10 -2 mg / L, it had a promoting effect on crop growth.

[0057] 3. Effects on plant flowering

[0058] Select healthy heading rice and wheat with similar growth conditions, transplant them into pots, 1 plant per pot, 10 pots per group, and repeat each group 3 times.

[0059] Prepare the same experimental solution as in Example 1, and use distilled water containing 0.1% dimethyl sulfoxide and 0.1% Tween-80 as the control group.

[0060] The example adopted the pot experiment method, spraying each group of potted plants. 10 main panicles with the same initial heading stage were selected for each treatment group, and fixed plants and panicles were observed. Starting from the first day of flowering, the flowering amount was recorded once a day. After each observation and recording of the data, the already opened glumes were cut off until the flowering ended, and the flowering rate was statistically calculated. The results are shown in Table 4. The calculation formula for the flowering rate is as follows:

[0061]

[0062] Table 4 Effects of Curcumin at Different Concentrations on the Flowering of Rice and Wheat

[0063]

[0064] Table 4 shows that curcumin had a certain effect on the flowering of both rice and wheat. Generally, when the concentration of the medicament was relatively high, it showed a significant inhibitory effect on the flowering of rice. When the concentration of the medicament was 2×10 -1 ~2×10 -3 mg / L, the flowering rate of the rice treatment group was significantly higher than that of the control group, and the flowering rate reached the highest under the treatment of 2×10 -2 mg / L of the medicament; there was no significant difference in the flowering rate between the treatment groups and the control group of wheat with different concentrations of the medicament, but the flowering rate was the lowest at the highest concentration, and the flowering rate was relatively high under the action of the medicament with a concentration of 2×10 -2 mg / L. Generally, there was a phenomenon of slightly inhibiting flowering at high concentrations. The effect of promoting the flowering of the two crops was the best under the action of the medicament with a concentration of 2×10 -2 mg / L.

[0065] Finally, it should be noted that the above specific embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit. Although the present invention has been described in detail with reference to the examples, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. Use of curcumin in enhancing the chlorophyll content of plants, wherein the dosage of curcumin is 2×10 -1 ~2×10 -3 mg / L; the plants are cereal plants and cruciferous plants; the cereal plants are rice and wheat, and the cruciferous plant is Shanghaiqing.

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