An anti-aging skin conditioning agent and its application

Abstract

The present invention provides an anti-aging skin conditioner and its application, wherein the anti-aging skin conditioner comprises a quezui tea extract and a peony root bark extract. The quezui tea extract of the present invention can significantly inhibit the increase of senescent proteins in fibroblasts induced by D-galactose amino acid (D-gal), exhibits excellent anti-aging potential, has excellent effect of inhibiting skin cell aging, and can significantly improve the aging condition of the skin. At the same time, the peony root bark extract can promote skin blood circulation, activate aging epidermal cells, and eliminate wrinkles. It is used in combination with the quezui tea extract, and the two have a synergistic effect in anti-aging efficacy, which can further improve the anti-aging effect of the skin conditioner.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to an anti-aging skin conditioning agent and application thereof. Background Art

[0002] Aging is an inevitable natural law in the process of life. It is affected by genetic factors, environmental factors and the complex morphological structure of the whole body. The mechanism of aging is still in the research stage. There are many theories of mechanism, such as somatic mutation theory, free radical theory, neuroendocrine regulation theory, gene attenuation theory, etc. In the process of aging, the living body forms intermolecular cross-linking of collagen, which not only reduces the elasticity and permeability of the skin, weakens the diffusion performance of nutrients and waste, but also reduces the solubility of collagen, making it difficult to be hydrolyzed by collagenase, resulting in decreased skin elasticity, wrinkles that are difficult to smooth and continue to deepen, thus accelerating the aging process of the skin. Therefore, skin care products that delay skin aging are becoming more and more popular.

[0003] CN111329810A discloses an antioxidant anti-aging cosmetic composition, which relates to the field of cosmetic technology. The antioxidant anti-aging cosmetic composition comprises the following components by weight: composite vegetable oil; niacinamide; yeast extract; mirabilis leaf / stem extract; sodium hyaluronate; carnosine; 3-o-ethyl ascorbic acid; ferulic acid. The invention realizes antioxidant functions from multiple levels and multiple pathways through the combined action of various components, and has multiple antioxidant effects such as actively scavenging free radicals and activating the human body to increase the content of enzymes that scavenges free radicals.

[0004] CN105030610A discloses an anti-aging composition, comprising a basic component and an anti-aging factor dispersed in the basic component, wherein the anti-aging factor is selected from at least three of soybean germ extract, wheat germ extract, safflower germ extract, and corn germ extract. At least three of the soybean germ extract, wheat germ extract, safflower germ extract, and corn germ extract produce a synergistic anti-aging effect with each other, and can synergistically promote collagen synthesis, quickly smooth skin fine lines, and repair damaged skin, thereby achieving the purpose of delaying skin aging.

[0005] However, the anti-aging effects of the above existing technologies are not obvious and cannot fundamentally solve the problem of skin aging. Based on this, it is necessary to develop a more effective anti-aging product to meet people's needs. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide an anti-aging skin conditioning agent and application thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides an anti-aging skin conditioner, comprising a Camellia sinensis extract and a Paeonia suffruticosa root bark extract.

[0009] The extract of quezui tea has the effects of dispelling wind and dampness, relaxing muscles and activating collaterals, and has a good therapeutic effect on high uric acid and its complications, and is mostly used in the treatment of diseases. The anti-aging skin conditioner of the present invention uses the extract of quezui tea because the staff of the present invention unexpectedly found that the extract of quezui tea can significantly inhibit the increase of senescent proteins in fibroblasts induced by D-galactose (D-gal), showing excellent anti-aging potential, and has excellent inhibitory effect on skin cell aging, which can significantly improve the aging condition of the skin. At the same time, the peony root bark extract can promote skin blood circulation, activate aging epidermal cells, and eliminate wrinkles. It is used in combination with the extract of quezui tea, and the two have a synergistic effect in anti-aging efficacy, which can further improve the anti-aging effect of the skin conditioner.

[0010] Preferably, the mass ratio of the Camellia oleifera extract to the Paeonia suffruticosa root bark extract is 1:(0.2-0.8), for example, it can be 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6 or 1:0.7, etc. Specific point values ​​within the above numerical ranges can be selected and will not be described one by one here.

[0011] In a second aspect, the present invention provides a use of the anti-aging skin conditioner as described in the first aspect in cosmetics.

[0012] Preferably, the cosmetics include essence, lotion, emulsion, cream or mask;

[0013] Preferably, the mass percentage of the anti-aging skin conditioner in the cosmetic is 1-15%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc. Specific point values ​​within the above numerical ranges can be selected and will not be described here one by one.

[0014] In a third aspect, the present invention provides an essence with anti-aging and moisturizing effects, wherein the essence with anti-aging and moisturizing effects comprises the anti-aging skin conditioner as described in the first aspect, a moisturizer, a thickener, an emollient, and deionized water.

[0015] The essence with anti-aging and moisturizing effects of the present invention can provide more nutrition and energy to skin cells through the combination of the anti-aging skin conditioner with moisturizers, emollients, thickeners and other components, thereby having a better effect of delaying skin aging, and can also achieve better effects of moisturizing and tightening the skin.

[0016] Preferably, the essence with anti-aging and moisturizing effects comprises, by weight, 1-15 parts of the anti-aging skin conditioner as described in the first aspect, 5-20 parts of moisturizer, 0.1-0.5 parts of thickener, 1-10 parts of emollient, and 1-90 parts of deionized water.

[0017] The amount of the anti-aging skin conditioner added to the essence with anti-aging and moisturizing effects of the present invention can be 2 parts, 4 parts, 5 parts, 6 parts, 8 parts, 10 parts, 12 parts or 14 parts, etc.;

[0018] The amount of the moisturizer added can be 6 parts, 7 parts, 9 parts, 10 parts, 12 parts, 15 parts, 17 parts or 19 parts, etc.;

[0019] The thickener may be added in an amount of 0.1, 0.2, 0.3, 0.4 or 0.5 parts, etc.;

[0020] The amount of the emollient added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0021] The added amount of the deionized water can be 5 parts, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts or 90 parts, etc.

[0022] Preferably, the moisturizing agent includes any one of glycerin, propylene glycol, butylene glycol, hexylene glycol or sodium hyaluronate, or a combination of at least two thereof.

[0023] Preferably, the moisturizer is a combination of glycerin, butylene glycol and sodium hyaluronate;

[0024] Preferably, the mass ratio of glycerol, butylene glycol and sodium hyaluronate is (2-5):(1-2):(0.01-1).

[0025] Wherein "2-5" can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0026] Wherein "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0027] Here, “0.01-1” can be 0.02, 0.03, 0.05, 0.1, 0.2, 0.4, 0.5, 0.8 or 0.9, etc.

[0028] Preferably, the thickener includes any one or a combination of at least two of carbomer, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, ammonium acryloyldimethyl taurate / VP copolymer or hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer.

[0029] Preferably, the emollient comprises any one of polydimethylsiloxane, squalane or caprylic / capric triglyceride or a combination of at least two thereof.

[0030] Preferably, the essence with anti-aging and moisturizing effects also includes a soothing and anti-inflammatory agent.

[0031] Preferably, the mass percentage of the soothing anti-inflammatory agent in the total amount of the essence with anti-aging and moisturizing effects is 1-5%, for example, it can be 1.5%, 2%, 2.5%, 3%, 3.5%, 4% or 4.5%.

[0032] Preferably, the soothing anti-inflammatory agent comprises any one of purslane extract, gardenia fruit extract, calendula extract, scutellaria oil, ophiopogon root extract or frankincense resin extract, or a combination of at least two thereof.

[0033] Preferably, the soothing anti-inflammatory agent is a combination of purslane extract, gardenia fruit extract and calendula extract.

[0034] In the present invention, the combination of the purslane extract, the gardenia fruit extract and the calendula extract can effectively and quickly regulate and relieve the inflammatory response and discomfort symptoms of the skin, thereby maintaining the skin in a stable and healthy state.

[0035] Preferably, the mass ratio of the purslane extract, the gardenia fruit extract and the calendula officinalis extract is (1-3):(0.1-1):(0.5-1.5).

[0036] Where "1-3" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5 or 2.8, etc.;

[0037] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0038] “0.5-1.5” can mean 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.

[0039] Preferably, the essence with anti-aging and moisturizing effects further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

[0040] In a fourth aspect, the present invention provides a method for preparing the essence with anti-aging and moisturizing effects as described in the third aspect, the preparation method comprising:

[0041] (1) Add deionized water, moisturizer, and thickener into a water phase pot, stir and heat up and keep warm; add emollient into an oil phase pot, stir and heat up and keep warm;

[0042] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsifying pot, homogenized, cooled, and the anti-aging skin conditioning agent as described in the first aspect is added and mixed evenly to obtain the product.

[0043] Preferably, the insulation temperature in step (1) is independently 75-80°C (for example, 75°C, 76°C, 77°C, 78°C or 79°C, etc.), and the insulation time is independently 1-10min (2min, 3min, 4min, 5min, 6min, 7min, 8min or 9min, etc.).

[0044] Preferably, the homogenization temperature in step (2) is 75-80°C (for example, 75°C, 76°C, 77°C, 78°C or 79°C, etc.), and the homogenization time is 5-10 min (for example, 5 min, 6 min, 7 min, 8 min, 9 min or 10 min, etc.).

[0045] Preferably, the temperature in step (2) is lowered to 35-45°C, for example, 36°C, 37°C, 38°C, 39°C, 40°C, 41°C, 42°C, 43°C or 44°C.

[0046] Preferably, step (2) further comprises adding any one or a combination of at least two of a soothing anti-inflammatory agent, a pH adjuster, a fragrance or a preservative.

[0047] Specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] (1) The quezui tea extract of the present invention can significantly inhibit the increase of senescent proteins in fibroblasts induced by D-galactose (D-gal), showing excellent anti-aging potential, having excellent inhibitory effect on skin cell aging, and can significantly improve the aging condition of the skin. At the same time, the peony root bark extract can promote skin blood circulation, activate aging epidermal cells, and eliminate wrinkles. When used in combination with the quezui tea extract, the two have a synergistic effect in anti-aging efficacy, which can further improve the anti-aging effect of the skin conditioner.

[0050] (2) The essence with anti-aging and moisturizing effects of the present invention can provide more nutrition and energy to skin cells through the combination of the anti-aging skin conditioner with moisturizers, emollients, thickeners and other components, thereby having a better effect of delaying skin aging, and can also have a better effect of moisturizing and tightening the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1This is a diagram showing the antioxidant effect of the essence in Test Example 2 in zebrafish. DETAILED DESCRIPTION

[0052] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0053] The quezui tea extract described in the embodiment of the present invention is prepared by the following method, which is:

[0054] (1) 100 g of dried Camellia sinensis tea was extracted by heating and reflux with 60% ethanol-water, with a solid-liquid ratio of 1:10 g / mL, an extraction temperature of 65°C, a time of 2 h, and two extraction times;

[0055] (2) The extract was concentrated under reduced pressure at 55°C until there was no alcohol taste, and then cooled at 4°C for 12 h. After the precipitate was fully separated, it was centrifuged at 3000 r / min for 15 min to collect the precipitate.

[0056] (3) The precipitate was heated and redissolved in distilled water, and loaded onto MCI-gelCHP 20P resin for separation and elution. The separation and elution steps were as follows: 2 BV of water elution, 5 BV of 20% ethanol-water elution, and 5 BV of 50% ethanol-water elution;

[0057] (4) Collect the 50% ethanol-water elution fraction, concentrate under reduced pressure at 50° C. to a solid content of 25%, and freeze-dry to obtain the product.

[0058] The peony root bark extract was purchased from Shanghai Jiyan Biotechnology Co., Ltd.

[0059] The purslane extract was purchased from Shanghai Jiyan Biotechnology Co., Ltd.

[0060] The marigold extract was purchased from Modern Biolande Biotechnology Co., Ltd.

[0061] The gardenia fruit extract was purchased from Shanghai Jiyan Biotechnology Co., Ltd.;

[0062] The acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer was purchased from Lubrizol Specialty Chemical Manufacturing (Shanghai) Co., Ltd., PEMULEN TR-1POLYMER;

[0063] The polydimethylsiloxane was purchased from Dow Chemical (Shanghai) Co., Ltd., PMX200-350cst.

[0064] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0065] Example 1

[0066] This embodiment provides an anti-aging skin conditioner, which is a Camellia sinensis extract and a Paeonia suffruticosa root bark extract in a mass ratio of 1:0.5.

[0067] Example 2

[0068] This embodiment provides an anti-aging skin conditioner, which is a Camellia sinensis extract and a Paeonia suffruticosa root bark extract in a mass ratio of 1:0.2.

[0069] Example 3

[0070] This embodiment provides an anti-aging skin conditioner, wherein the anti-aging skin conditioner is a Camellia sinensis extract and a Paeonia suffruticosa root bark extract in a mass ratio of 1:0.8.

[0071] Example 4

[0072] This embodiment provides an anti-aging skin conditioner, which is a Camellia sinensis extract and a Paeonia suffruticosa root bark extract in a mass ratio of 1:1.

[0073] Example 5

[0074] This embodiment provides an anti-aging skin conditioner, wherein the anti-aging skin conditioner is a Camellia sinensis extract and a Paeonia suffruticosa root bark extract in a mass ratio of 1:0.1.

[0075] Comparative Example 1

[0076] This comparative example provides an anti-aging skin conditioning agent, wherein the anti-aging skin conditioning agent is only a Camellia sinensis extract.

[0077] Comparative Example 2

[0078] This comparative example provides an anti-aging skin conditioner, wherein the anti-aging skin conditioner is only the peony root bark extract.

[0079] Comparative Example 3

[0080] This comparative example provides an anti-aging skin conditioner, which is a Camellia sinensis extract and a grape seed extract in a mass ratio of 1:0.5.

[0081] Application Example 1

[0082] This application example prepares an essence with anti-aging and moisturizing effects, and its formula is as follows:

[0083]

[0084] Its preparation method is:

[0085] (1) Add deionized water, moisturizer, and thickener into a water phase pot, stir and heat to 80°C and keep warm for 5 minutes; add emollient into an oil phase pot, stir and heat to 80°C and keep warm for 5 minutes;

[0086] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsifying pot, homogenized at 80° C. for 10 minutes, cooled to 40° C., and the skin conditioner as in Example 1 is added and mixed evenly to obtain the product.

[0087] Application Example 2-5

[0088] This application example provides five essences with anti-aging and moisturizing effects. The only difference between their formulas and those of Application Example 1 is that the skin conditioners of Example 1 are replaced with skin conditioners of Examples 2-5 with the same added amounts, and other conditions remain unchanged. The preparation method refers to that of Application Example 1.

[0089] Application Example 6

[0090] This application example provides an essence with anti-aging and moisturizing effects. The formula of this essence differs from that of application example 1 only in that the moisturizer does not contain glycerin, and the reduced amount thereof is distributed to butylene glycol and sodium hyaluronate in proportion to the number of parts, while the other ingredients and contents remain unchanged. The preparation method is the same as that of application example 1.

[0091] Application Example 7

[0092] This application example provides an essence with anti-aging and moisturizing effects, the formula of which is different from that of application example 1 only in that the moisturizer does not contain butylene glycol, and the reduced amount thereof is distributed to glycerol and sodium hyaluronate in proportion, while the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0093] Application Example 8

[0094] This application example provides an essence with anti-aging and moisturizing effects, the formula of which is different from that of application example 1 only in that the moisturizer does not contain sodium hyaluronate, and the reduced amount thereof is distributed to glycerol and butylene glycol in proportion, while the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0095] Application Example 9

[0096] This application example prepares an essence with anti-aging and moisturizing effects, and its formula is as follows:

[0097]

[0098]

[0099] Its preparation method is:

[0100] (1) Add deionized water, moisturizer, and thickener into a water phase pot, stir and heat to 80°C and keep warm for 5 minutes; add emollient into an oil phase pot, stir and heat to 80°C and keep warm for 5 minutes;

[0101] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsification pot, homogenized at 80°C for 10 minutes, cooled to 40°C, and the skin conditioner and soothing antioxidant are added in sequence and mixed evenly.

[0102] Application Example 10

[0103] This application example provides an essence with anti-aging and moisturizing effects. The formula of this essence is different from that of application example 9 only in that the soothing anti-inflammatory agent does not contain purslane extract, and its reduced amount is distributed to gardenia fruit extract and calendula extract in proportion, while other ingredients and contents remain unchanged. The preparation method is referred to application example 10.

[0104] Application Example 11

[0105] This application example provides an essence with anti-aging and moisturizing effects. The formula of this essence is different from that of application example 9 only in that the soothing anti-inflammatory agent does not contain gardenia fruit extract, and its reduced amount is distributed to purslane extract and calendula extract in proportion, while other ingredients and contents remain unchanged. The preparation method is referred to application example 10.

[0106] Application Example 12

[0107] This application example provides an essence with anti-aging and moisturizing effects. The formula of this essence is different from that of application example 9 only in that the soothing anti-inflammatory agent does not contain calendula extract, and its reduced amount is distributed to purslane extract and gardenia fruit extract in proportion, while other ingredients and contents remain unchanged. The preparation method is referred to application example 10.

[0108] Comparative Application Examples 1-3

[0109] This comparative application example provides two serums with anti-aging and moisturizing effects, the formula of which is different from that of application example 1 only in that the anti-aging skin conditioning agent of example 1 is replaced with the skin conditioning agents of comparative examples 1-3 with the same addition amount, and the other ingredients and contents remain unchanged. The preparation method is referred to application example 1.

[0110] Comparative Application Example 4

[0111] This comparative application example provides an essence with anti-aging and moisturizing effects, the formula of which differs from that of application example 1 only in that it does not contain the anti-aging skin conditioning agent, and the other ingredients and contents remain unchanged. The preparation method is similar to that of application example 1.

[0112] Test Example 1

[0113] Experiment on promoting elastin production

[0114] The serum with anti-aging and moisturizing effects provided in Example 1-12 and the serum with anti-aging and moisturizing effects provided in Comparative Example 1-4 were used to conduct an experiment to promote elastin production, and the method was as follows:

[0115] (1) Human fibroblasts were seeded on a 96-well plate at a density of 20,000 cells per well, and culture medium was added. The plates were cultured in a 37°C incubator for 24 hours. After the cells adhered to the wall, new culture medium was replaced, and each test sample (the final concentration of the control sample was 50 mg / mL) was added. A 20 mmol / L PBS solution was used as a blank control, and the plates were cultured in a 37°C incubator for 2 days.

[0116] (2) After the culture is completed, the cells are washed with cell lysis buffer and ultrasonically disrupted. The content of elastin is detected using the Biocolor elastin detection kit: the sample is treated with an elastin precipitant to precipitate the elastin, centrifuged, the supernatant is removed, the dye and reaction solution are added to the precipitate to react to form an elastin-dye complex, centrifuged and washed three times with PBS solution to remove unbound dye, a dye releaser is added to release the dye in the elastin-dye complex, the absorbance of the sample at 513 nm is measured using a spectrophotometer, and the expression level of elastin is calculated based on the absorbance-dye concentration standard curve (the expression level of the blank group is taken as 1);

[0117] The specific test results are shown in Table 1 below:

[0118] Table 1

[0119] Test samples Elastin expression Test samples Elastin expression Application Example 1 6.8 Application Example 9 6.4 Application Example 2 6.7 Application Example 10 6.7 Application Example 3 6.5 Application Example 11 6.6 Application Example 4 5.2 Application Example 12 6.3 Application Example 5 5.4 Comparative application example 1 3.5 Application Example 6 5.8 Comparative Application Example 2 2.9 Application Example 7 6.5 Comparative Application Example 3 5.5 Application Example 8 6.6 Comparative Application Example 4 2.0

[0120] From the test data in Table 1, it can be seen that the expression of elastin in the essence with anti-aging and moisturizing effects provided in Application Examples 1-3 of the present invention is above 6.5, while the expression of elastin in the essence with anti-aging and moisturizing effects in Comparative Application Example 4 is only 2.0, which indicates that the addition of anti-aging skin conditioners to the essence with anti-aging and moisturizing effects of the present invention can stimulate cell growth, increase skin elasticity, and thus improve skin condition. The expression of elastin in the essence with anti-aging and moisturizing effects described in Comparative Application Examples 1-2 is only 3.5 or 2.9, which fully indicates that the tea extract and the peony root bark extract cooperate with each other and have a synergistic effect in promoting the expression of elastin.

[0121] Test Example 2 Antioxidant Test

[0122] The essences of Example 1 and Comparative Application Examples 1-4 were used to perform ROS scavenging tests in zebrafish embryos.

[0123] Experimental organisms: 2 dpf zebrafish larvae

[0124] Test method: Select 2dpf zebrafish larvae with normal development and place them in a six-well cell culture plate, with 10 larvae per well. Remove the embryo culture medium in the six-well plate without harming the larvae, and quickly add 3mL of 0.1% test dilution to each well. Wrap the six-well cell culture plate with aluminum foil and incubate it in a biochemical incubator (28.5℃±1.0℃) in the dark for 20h. After the incubation, wash off the test culture medium, replace it with the embryo culture medium containing the active oxygen test solution (CM-H2DCFD detection reagent), incubate for 2h±1h, wash off the culture medium, place the zebrafish sideways, and take pictures of the larvae under a fluorescent stereo microscope according to uniform photography parameters. .

[0125] The calculation method of reactive oxygen species (ROS) clearance rate is as follows:

[0126]

[0127] In the above formula, T is the average value of the "average signal intensity" of ROS in the fish embryos of the test substance treatment group, and C is the average value of the "average signal intensity" of ROS in the fish embryos of the blank control group.

[0128] The specific test results are shown in Table 2 and Figure 1 As shown:

[0129] Table 2

[0130] Test samples ROS clearance rate % Application Example 1 85 Comparative application example 1 60 Comparative Application Example 2 30 Comparative Application Example 3 70 Comparative Application Example 4 20 Blank control group 0

[0131] As shown in Table 2, the essence with anti-aging and moisturizing efficacy prepared in Application Example 1 has a scavenging rate of 85% for ROS, indicating that the components in the essence with anti-aging and moisturizing efficacy of the present invention cooperate with each other, synergistically enhance the effect, and have a good antioxidant effect. As shown in Comparative Example Application Examples 1-2, the tea extract and the peony root bark extract in the anti-aging skin conditioner cooperate with each other, which can significantly enhance the antioxidant effect of the essence with anti-aging and moisturizing efficacy.

[0132] Test Example 3

[0133] Anti-wrinkle effect experiment

[0134] The anti-wrinkle effect experiment was conducted on the essence with anti-aging and moisturizing effects provided in Example 1-5 and the essence with anti-aging and moisturizing effects provided in Comparative Example 1-4. The specific test method is as follows:

[0135] (1) Subjects: 90 female volunteers aged 35-55 with healthy skin were randomly divided into 9 groups (5 males and 5 females in each group);

[0136] (2) Experimental instruments: Skin wrinkle tester VL650 produced by German CK company;

[0137] (3) Test method: A skin wrinkle tester VL650 produced by German CK Company was used and a silicone sleeve was used to measure the wrinkle depth on the forehead of the subject. The forehead wrinkles of the subject were tested respectively, and the average value of the test data was taken for three times. The specific method of using the essence with anti-aging and moisturizing effects was as follows: cleansing the face at 8 am, and after cleansing, 0.5±0.05 g of each test sample essence was applied to the face; cleansing the face at 8 pm, and after cleansing, 0.5±0.05 g of each test sample essence was applied to the face. The experimental period lasted for 60 days. Finally, the percentage of wrinkle depth reduction after 30 days and 60 days of treatment was calculated.

[0138] The specific test results are shown in Table 3 below:

[0139] Table 3

[0140]

[0141] It can be seen from the test data in Table 3 that the percentage of wrinkle depth reduction after 60 days of the essence with anti-aging and moisturizing effects provided by Application Examples 1-3 of the present invention is more than 24%, which fully demonstrates that the various components in the essence with anti-aging and moisturizing effects cooperate with each other and synergize to effectively fight skin wrinkles and slow down the skin aging process, reduce the generation of static lines, and improve the dynamic lines of the skin by promoting the expression of skin elastin and the proliferation and growth of cells.

[0142] Test Example 4

[0143] Moisturizing performance test:

[0144] The essences with anti-aging and moisturizing effects prepared in Application Examples 1 and 6-8 were tested, and the changes in skin moisture content of the test subjects were compared before and after use (the usage operation was kept consistent). A total of 40 volunteers were included, including 28 females and 12 males, aged 20-40 years old with an average age of 28 years old. They were randomly divided into 4 groups, with 7 females and 3 males in each group.

[0145] The subjects washed their forearms and sat quietly for 30 minutes in a set humidity environment. A 3×3 cm 2 The left arm was used as the test area for the test sample, and the symmetrical area on the right arm was used as the blank control. The moisture content of each experimental part was detected by Corneometer CM825. The test was repeated 3 times and the average value was recorded.

[0146] The formula for calculating skin hydration rate is:

[0147] The average skin hydration rate of the subjects after using the samples for 2 hours is shown in Table 4:

[0148] Table 4

[0149] Group Skin hydration rate Application Example 1 45.6％ Application Example 6 35.8％ Application Example 7 37.7％ Application Example 8 38.2％

[0150] It can be seen from the data in Table 4 that the essence with anti-aging and moisturizing effects of the present invention has excellent moisturizing effects; the three ingredients of glycerin, butylene glycol and sodium hyaluronate in the moisturizer have a certain synergistic effect on the above effects, and the combination of the three is indispensable.

[0151] Test Example 5: Anti-inflammatory efficacy test

[0152] Test sample: the essence with anti-aging and moisturizing effects provided in Application Examples 1 and 9-12;

[0153] The test method is as follows:

[0154] (1) SLS-induced 3D epidermal model to detect inflammatory factors

[0155] After the 3D epidermal model is stably cultured, an induction group, a sample group, and a blank control group are set up; 0.3% SLS is added to the epidermal models of the induction group and the sample group, and induced for 5-30 minutes at 37°C, 5% CO2, and saturated humidity. After being thoroughly rinsed with DPBS buffer, the essence is applied to the surface of the epidermal model and incubated for 36-48 hours. The cultured culture medium is collected, and the IL-1α and IL-8 contents are detected using an ELISA kit. The operating steps are consistent with the instructions for the kit. The standard curve is calculated using the software, and the relative expression is calculated with the induction control group as 100%. Relative expression = sample group content / induction control group content

[0156] (2) UV-induced 3D epidermal model to detect inflammatory factors

[0157] After the 3D epidermal model is stably cultured, an induction group, a sample group and a blank control group are set up; the epidermal models of the induction group and the sample group are irradiated with UV using an ultraviolet solar simulator, and the irradiation amount is monitored using a UV-meter, and the UVA dose is 5-20J, and the UVB dose is 50-200mJ. The epidermal models are moved one by one into the well plate with culture medium for post-incubation for 36-48h. The post-cultured culture medium is collected, and the PGE2 content is detected using an ELISA kit. The operating steps are consistent with the instructions for the kit. The standard curve is calculated using the software, and the relative expression is calculated with the induced control group as 100%, and the relative expression = sample group content / induced control group content.

[0158] (3) LPS-induced Raw264.7 cells to detect inflammatory factors

[0159] (3.1) MTT assay and analysis of cytotoxicity of test substances

[0160] Mouse macrophages RAW264.7 with good cell morphology and in the logarithmic growth phase were collected and inoculated into 96-well plates for overnight culture. After gradient dilution of the essence, it was added to the cells for post-incubation, and the MTT assay was performed on the cells. The absorbance at a wavelength of 570nm was read on the microplate reader, and 630nm was used as the reference wavelength. The measurement results were recorded and the cell activity was calculated. Cell survival rate = [(sample group-blank group) / (control group-blank group)] x 100%, and the concentration with cell activity greater than 90% was selected for subsequent anti-inflammatory tests.

[0161] (3.2) Detection of TNF-α, IL-6, and NO secretion in the supernatant of LPS-induced RAW264.7 cell inflammation model

[0162] RAW264.7 cells were inoculated in a 24-well cell culture plate and cultured overnight. A blank control group, an LPS-induced group, and a sample group were set up. Except for the blank control group, the LPS-induced group and the sample group were added with LPS to a final concentration of 1 μg / mL, and cultured for 24 hours at 37°C, 5% CO2, and saturated humidity. The cell culture medium was collected, and the TNF-α and IL-6 content of the supernatant was detected using a Lianke mouse ELISA kit, and the NO content of the supernatant was detected using a Griess reagent. The operation steps were referred to the instructions of the kit. The standard curve was calculated using data software, and the content of TNF-α, IL-6, and NO was calculated, and the relative expression was calculated with the LPS-induced group as 100%.

[0163] The above-mentioned TNF-α, IL-6, IL-1α, IL-8 and PGE2 inflammatory factors and NO were analyzed and analyzed using One-way ANOVA. All statistical analyses were two-tailed. p<0.05 was considered to be significantly different, indicated by "*", p<0.01 was considered to be very significantly different, indicated by "**", and p<0.001 was considered to be extremely significantly different, indicated by "***".

[0164] The experimental significant differences of the 6 test substances detected in this example at the cellular level and 3D skin level are summarized in Table 5. Evaluation and scoring are performed according to the standards in Table 6. The anti-inflammatory efficacy evaluation results of the test substances are shown in Table 7.

[0165] Table 5

[0166]

[0167]

[0168] Table 6

[0169]

[0170] Table 7

[0171]

[0172] The anti-inflammatory effects of the test substances were ranked according to the summary scores, and it was found that compared with Application Example 1, Application Examples 9 and 10-12 were higher than Application Example 1 in the comprehensive scores, and the anti-inflammatory and soothing effects were stronger than Application Example 1. This shows that the comparison between Application Example 9 and Application Examples 10-12 fully illustrates that the purslane extract, gardenia fruit extract and calendula extract in the soothing anti-inflammatory agent of the present invention cooperate with each other, have a synergistic effect, can effectively inhibit the release and transmission of multiple inflammatory factors, can play an anti-inflammatory and soothing role, and thus effectively repair inflamed skin.

[0173] Test Example 6: Red blood cell hemolysis test

[0174] The red blood cell haemolysis test (RBC) is one of the alternative methods to the rabbit eye irritation test. The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of cell hemoglobin dissolution and the degree of denaturation. The RBC test can be used to evaluate the eye irritation of cosmetics.

[0175] The serum with anti-aging and moisturizing effect prepared in Example 1-12 was subjected to red blood cell hemolysis experiment. The experiment adopted the RBC experimental test method and judgment standard of the European Alternative Method Validation Center. The specific judgment standard is shown in Table 8, where HD 50 is the sample concentration when 50% of red blood cells are hemolyzed, DI is the protein denaturation index, and L / D is HD 50 Ratio to DI.

[0176] Table 8

[0177] L / D Grading L / D>100 Non-irritating 10﹤L / D≦100 Slightly irritating 1﹤L / D≦10 Mild irritant 0.1﹤L / D≦1 Moderate irritation L / D≦0.1 Severe irritation

[0178] The irritation results of the samples prepared in Application Examples 1-13 are shown in Table 9.

[0179] Table 9

[0180]

[0181]

[0182] From the sample irritation results in Table 9, it can be seen that the irritation classification of the essence with anti-aging and moisturizing effects prepared in Application Examples 1-12 of the present invention is non-irritating, indicating that each component in the essence with anti-aging and moisturizing effects of the present invention is mild and non-irritating.

[0183] The applicant declares that the present invention illustrates an anti-aging skin conditioning agent and its application through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0184] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0185] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. An anti-aging skin conditioning agent, characterized in that The anti-aging skin conditioner includes a camellia sinensis extract and a peony root bark extract; The mass ratio of the quezui tea extract to the peony root bark extract is 1:(0.2-0.8).

2. Use of the anti-aging skin conditioning agent according to claim 1 in cosmetics.

3. The use according to claim 2, characterized in that The cosmetics include essence, lotion, emulsion, cream or mask.

4. The use according to claim 2, characterized in that The mass percentage of the anti-aging skin conditioner in the cosmetic is 1-15%.

5. An essence with anti-aging and moisturizing effects, characterized in that: The essence with anti-aging and moisturizing effects comprises the anti-aging skin conditioner as claimed in claim 1, a moisturizer, a thickener, an emollient, and deionized water.

6. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The essence with anti-aging and moisturizing effects comprises, by weight, 1-15 parts of the anti-aging skin conditioner according to claim 1, 5-20 parts of moisturizer, 0.1-0.5 parts of thickener, 1-10 parts of emollient, and 1-90 parts of deionized water.

7. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The moisturizing agent includes any one of glycerin, propylene glycol, butylene glycol, hexylene glycol or sodium hyaluronate, or a combination of at least two of them.

8. The essence with anti-aging and moisturizing effects according to claim 7, characterized in that: The moisturizer is a combination of glycerin, butylene glycol and sodium hyaluronate.

9. The essence with anti-aging and moisturizing effects according to claim 8, characterized in that: The mass ratio of the glycerol, butylene glycol and sodium hyaluronate is (2-5): (1-2): (0.01-1).

10. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The thickener includes any one of carbomer, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, ammonium acryloyldimethyl taurate / VP copolymer or hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer or a combination of at least two thereof.

11. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The emollient includes any one of polydimethylsiloxane, squalane or caprylic / capric triglyceride or a combination of at least two thereof.

12. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The essence with anti-aging and moisturizing effects also includes a soothing and anti-inflammatory agent.

13. The essence with anti-aging and moisturizing effects according to claim 12, characterized in that: The mass percentage of the soothing anti-inflammatory agent in the total amount of the essence with anti-aging and moisturizing effects is 1-5%.

14. The essence with anti-aging and moisturizing effects according to claim 12, characterized in that: The soothing anti-inflammatory agent includes any one of purslane extract, gardenia fruit extract, calendula extract, sphenanthera oil, ophiopogon root extract or frankincense resin extract or a combination of at least two thereof.

15. The essence with anti-aging and moisturizing effects according to claim 14, characterized in that: The soothing anti-inflammatory agent is a combination of purslane extract, gardenia fruit extract and calendula extract.

16. The essence with anti-aging and moisturizing effects according to claim 15, characterized in that: The mass ratio of the purslane extract, the gardenia fruit extract and the calendula extract is (1-3): (0.1-1): (0.5-1.5).

17. The essence with anti-aging and moisturizing effects according to claim 5, characterized in that: The essence with anti-aging and moisturizing effects further comprises any one of a pH regulator, a preservative or a fragrance, or a combination of at least two of them.

18. The method for preparing the essence with anti-aging and moisturizing effects according to any one of claims 5 to 11, characterized in that: The preparation method comprises: (1) Add deionized water, moisturizer, and thickener to the water phase pot, stir and heat up and keep warm; add emollient to the oil phase pot, stir and heat up and keep warm; (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsifying pot, homogenized, cooled, and the anti-aging skin conditioning agent as claimed in claim 1 is added and mixed evenly to obtain the product.

19. The method for preparing the essence with anti-aging and moisturizing effects according to claim 18, characterized in that: The insulation temperature in step (1) is independently 75-80° C., and the insulation time is independently 1-10 min.

20. The method for preparing the essence with anti-aging and moisturizing effects according to claim 18, characterized in that: The homogenization temperature in step (2) is 75-80°C, and the homogenization time is 5-10 minutes.

21. The method for preparing the essence with anti-aging and moisturizing effects according to claim 18, characterized in that: Step (2) is cooling to 35-45°C.

22. The method for preparing the essence with anti-aging and moisturizing effects according to claim 18, characterized in that: Step (2) further comprises adding any one or a combination of at least two of a soothing anti-inflammatory agent, a pH adjusting agent, a fragrance or a preservative.

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