Method for setting up a biofloc culture environment
By installing multiple microporous aeration pipes at the bottom of the aquaculture pond and continuously aerating, the problem of floc settling due to dissolved oxygen shortage was solved, and the continuous tumbling of the water and the increase of dissolved oxygen were achieved, ensuring the suspension of the biological flocs and the stability of water quality.
Patent Information
- Application Number
- CN202310505003.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-06
- Publication Date
- 2025-10-17
- Estimated Expiration
- 2043-05-06
AI Technical Summary
In existing biofloc culture technology, dissolved oxygen shortages cause the flocs to sink to the bottom and die, affecting water quality and aquaculture results.
Multiple aeration pipes are installed at the bottom of the aquaculture pond, including the first aeration pipe near the pond wall, the ring aeration pipe in the center of the pond, the fifth aeration pipe on the inner side wall of the pond, and the third and fourth aeration pipes at the bottom of the pond. All of them have a microporous structure and are continuously aerated by an air pump to create water tumbling and keep the biological flocs in suspension.
By continuously aerating the water to keep it turbulent, the sedimentation of bioflocs is prevented, the dissolved oxygen concentration is increased, and the water treatment effect and the healthy growth of aquaculture products are ensured.
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Figure CN116813107B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of biological floc culture, and particularly relates to a method for setting a biological floc culture environment. BACKGROUND
[0002] The biological floc is formed by heterotrophic bacteria and algae under controllable conditions, and is used for degrading ammonia nitrogen, nitrous acid nitrogen and organic carbon in water by microorganisms, converting them into bacterial proteins, and simultaneously using the floc itself as bait organisms for aquatic animals to form a microcycle, so as to control water quality and achieve secondary utilization of nutrients.
[0003] The in-situ water treatment of the aquaculture tail water can be achieved by the aquaculture mode based on the biological floc technology (Biofloc Aquaculture System, BAS), so as to achieve the standards of water saving, emission reduction and even zero water exchange, and the microbial environment can achieve disease prevention and control in aquaculture, which is one of the ways to solve the problems of resources and environment. The mode is gradually popularized in commercial aquaculture of shrimps and tilapias, and the biological floc is used for water treatment of the aquaculture system in the process of water treatment, which is mostly ex-situ aquaculture tail water treatment, is applied to freshwater aquaculture, and the biological floc is added in the process of aquaculture.
[0004] However, the biological floc aquaculture technology in the prior art often causes the floc to sink to the bottom and die due to the shortage of dissolved oxygen in the actual application process, which affects the water treatment effect of the biological floc, so as to increase the nitrite and ammonia nitrogen in the water body and affect the aquatic products in the pond. SUMMARY
[0005] The present application aims to provide a method for setting a biological floc culture environment, comprising the steps of:
[0006] A plurality of first aeration pipes are arranged near the side wall of the pool body at the bottom of the pool body accommodating the aquaculture water body, the distance between the first aeration pipe and the inner wall of the pool body is within 0.5 meters, both ends of each first aeration pipe are closed, and the pipe body of each first aeration pipe is communicated with the air outlet of the air pump;
[0007] The pipe body of the first aeration pipe is a microporous gas permeable structure;
[0008] The air pump is arranged to continuously aerate.
[0009] Further, the method further comprises the steps of:
[0010] An annular second aeration pipe is arranged at the center position of the bottom of the pool body, both ends of the annular second aeration pipe are communicated, the pipe body of the second aeration pipe is communicated with the air outlet of the air pump, and the pipe body of the second aeration pipe is a microporous gas permeable structure.
[0011] Further, the method further comprises the steps of:
[0012] A plurality of third aeration pipes are arranged in parallel in the pool body; a plurality of fourth aeration pipes in communication with the third aeration pipes are equidistantly arranged on the pipe body of each third aeration pipe, and the other end of the fourth aeration pipe is vertically arranged on the bottom of the pool body;
[0013] A fifth aeration pipe is arranged along the inner side wall of the pool body; one end of the fifth aeration pipe is closed, the other end of the fifth aeration pipe is in communication with the air pump, and the pipe body of the fifth aeration pipe is in communication with both ends of each third aeration pipe.
[0014] Further, the pool body is arranged in a square or circular shape.
[0015] Further, the pipe diameter of the first aeration pipe is 0.8-1.6 cm, and the length is 1.75 m.
[0016] Further, the fourth aeration pipe is provided with an oxygen stone at the end close to the pool bottom.
[0017] Further, the third aeration pipes are equidistantly arranged.
[0018] Further, the pipe diameter of the fifth aeration pipe is 32 mm, and the pipe diameter of the fourth aeration pipe is 0.5-1 cm.
[0019] Further, the pipe spacing of the third aeration pipe is 0.8-1 m.
[0020] Further, the rotating speed of the oxygen pump is 1400-1500 r / min.
[0021] The present application can keep the water in the pool in a rolling state without interruption, keep the biological floc in a suspended state in the breeding system, and increase the dissolved oxygen of the water body. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 The figure is a schematic diagram of an embodiment of the present application; the arrows in the figure show the flow direction of the water body under the action of the gas and the inner wall of the pool body;
[0023] Figure 2 The figure is a schematic diagram of another embodiment of the present application;
[0024] Marked legend:
[0025] Pool body 1, bottom wall 11, central part 111 of the bottom wall, inner side wall 12, first aeration pipe 2, plug 21, three-way valve 3, air pump 4, annular second aeration pipe 5, fifth aeration pipe 6, air outlet hole 7, third aeration pipe 8, fourth aeration pipe 9, oxygen stone 10. DETAILED DESCRIPTION
[0026] First, the related technology is explained and described.
[0027] Biofloc is a flocculent material formed by flocculation of organic matter, inorganic matter, protozoa and algae in water body, with heterotrophic microorganisms as the main body, and is composed of flocculent insoluble substances such as bacterial flocs, filamentous bacteria, attached microbial extracellular products, extracellular polymers, intracellular products (poly-β-hydroxybutyric acid, polyphosphate, polysaccharides, etc.), heterotrophic bacteria, nitrifying bacteria, denitrifying bacteria, algae, fungi, protozoa, etc.
[0028] The source of the biofloc for culture according to the present application includes: freshwater biofloc cultivated by the biofloc reactor recorded in the literature (Liu Wenchang, Luo Guozhi, Tan Hongxin, et al. Treatment effect of bioflocculation reactor on wastewater in pilot-scale recirculating aquaculture system; Transactions of the Chinese Society of Agricultural Engineering; 2016, 32(08)), and also includes biofloc adapted to seawater culture obtained by seawater acclimation of the above-mentioned source of freshwater biofloc.
[0029] The following examples further illustrate the present application, but should not be construed as limiting the present application. Modifications or substitutions of the method, steps or conditions of the present application, without departing from the spirit and essence of the present application, all belong to the scope of the present application.
[0030] Some embodiments include the steps of:
[0031] A plurality of first aeration pipes are arranged near the side wall of the pool body at the bottom of the pool body accommodating the aquaculture water body, the distance between the first aeration pipe and the inner wall of the pool body is within 0.5 meters, both ends of each first aeration pipe are closed, and the pipe body of each first aeration pipe is communicated with the air outlet of the air pump; the pipe body of the first aeration pipe is of a microporous structure,
[0032] A water outlet is arranged at the middle position of the bottom of the pool body;
[0033] The air pump is arranged to continuously aerate;
[0034] An annular second aeration pipe is arranged at the center position of the bottom of the pool body, both ends of the annular second aeration pipe are communicated, and the pipe body of the second aeration pipe is communicated with the air outlet of the air pump; the pipe body of the second aeration pipe is of a microporous structure.
[0035] Preferably, eight first aeration pipes are arranged in the pool body, the pipe diameter of the first aeration pipe is 0.8-1.6 cm, and the length is 1.75 m.
[0036] Preferably, the first aeration pipe and the second aeration pipe are microporous nanometer aeration pipes.
[0037] After the air pump is started to fill in the gas, the air meets the inner wall and flows back to rotate, so that the water in the pool water body is stirred in one direction. When the biological flocculation rotates to the center position with the water body, the water body is driven to roll by the annular aeration pipe at the center position of the pool body bottom, so as to prevent the biological flocculation from depositing and causing death.
[0038] Some embodiments include the steps of:
[0039] A plurality of first aeration pipes are arranged near the side wall of the pool body at the bottom of the pool body accommodating the culture water body, the distance between the first aeration pipe and the inner wall of the pool body is within 0.5 meters, both ends of each first aeration pipe are closed, and the pipe body of each first aeration pipe is communicated with the air outlet of the air pump; the pipe body of the first aeration pipe is a microporous structure;
[0040] A water outlet is arranged at the middle position of the pool body bottom;
[0041] The air pump is arranged to continuously aerate;
[0042] A plurality of third aeration pipes are arranged in parallel at a height of at least 0.5 meters from the pool body bottom in the pool body; a plurality of fourth aeration pipes are equally arranged on the pipe body of each third aeration pipe and communicated with the third aeration pipe, and the other end of the fourth aeration pipe is vertically arranged on the pool body bottom;
[0043] A fifth aeration pipe is arranged along the inner side wall of the pool body in the pool body, one end of the fifth aeration pipe is closed, the other end of the fifth aeration pipe is communicated with the air pump, and the pipe body of the fifth aeration pipe is communicated with both ends of each third aeration pipe;
[0044] Preferably, the third aeration pipes are equally arranged, the end of the fourth aeration pipe close to the pool bottom is provided with an oxygen stone, the pipe diameter of the fourth aeration pipe is 0.5-1 cm, and the pipe diameter of the fifth aeration pipe is 32 mm.
[0045] Preferably, the third aeration pipe is a PVC pipe, the fourth aeration pipe is a plastic hose, and the fifth aeration pipe is a PVC pipe.
[0046] Some embodiments, the pool body is arranged as a square or a circle.
[0047] Some embodiments, the air pump rotation speed is 1400-1500 r / min.
[0048] The technical solutions of the present application are further explained below by examples and in conjunction with the drawings.
[0049] Example 1
[0050] As Figure 1As shown, the tank body 1 is a 6m x 6m square cement tank. Eight first aeration pipes 2, each 1.75m long and 0.8-1.6cm in diameter, are fixed to the bottom wall 11 of the tank body 1, 0.5m away from the inner wall 12 of the tank body 1. Both ends of the first aeration pipes 2 are fitted with plugs 21, and the pipes are connected to the air pump outlet via a three-way valve 3. A ring 5 with a radius of 0.4m is formed by the first aeration pipes 2 at the center 111 of the bottom 11 of the tank body 1. The second aeration pipes 5 are fixedly connected at both ends via a three-way valve 3. The three-way valve 3 is also connected to the outlet of an air pump 4, which is used to inject air into the tank body 1. The first and second aeration pipes are microporous nano-aeration pipes.
[0051] After starting the air pump to fill the tank with gas, the air encounters the inner wall and flows back and forth, causing the water in the pool to roll and stir in one direction. When the biological flocs rotate to the center with the water, the annular aeration pipe located at the center of the bottom of the pool is inflated to drive the water to roll. The upward movement of the gas causes the water to flow to the surface and roll to both sides. The gas on the side close to the inner wall of the pool encounters the force of the inner wall and rotates and rolls again to form a backwash water flow.
[0052] Example 2
[0053] like Figure 2 As shown, eight sections of first aeration pipes 2 with a length of 1.5 meters and a diameter of 1.6 centimeters are fixed to the bottom 11 in the tank body 1, 0.5 meters away from the inner wall 12; both ends of the first aeration pipe 2 are provided with a plug 21, and the pipe body is provided with an air outlet connected to the air outlet of the air pump 4 through a three-way valve 3. The inner wall of the upper part of the tank body 1 is fixed with a fifth aeration pipe 6 with a diameter of 32 mm PVC on both opposite sides (the PVC pipe 6 is connected to the workshop aeration main pipeline, and the workshop main pipeline is equipped with a Roots blower (vacuum pump) HDSR175A), and one end is provided with a plug (not shown in the figure). ), and the other end is connected to the air outlet of the air pump 4; the outer wall of the PVC tube 6 is provided with a plurality of air outlet holes 7 1 m apart, and the air outlet holes 7 are respectively connected to the two ends of the third aeration tube 8 with a diameter of 0.8 cm and arranged in parallel. The tube body of the third aeration tube 8 is connected to a fourth aeration tube 9 with a length of 0.5-1 m, and the fourth aeration tube 9 is vertically arranged on the bottom wall 11 of the pool body; the other end of the fourth aeration tube 9 is connected to an oxygen stone 10. After the gas is input, the tiny pores on the oxygen stone generate bubbles to make the water body evenly turn upward, drive the biological flocs to stir and roll, and prevent the biological flocs from settling.
[0054] By adopting the methods of Example 1 and Example 2, nitrite and ammonia nitrogen in the aquaculture water containing biofloc can be treated, and aquatic products (in some embodiments, redfin pufferfish or hybrid pufferfish) cultured in the pond can survive normally.
[0055] It should be noted that the example combining part or all of the technologies of Example 1 and Example 2 is also within the optional range of the embodiments of the present application.
[0056] Oxygen stone, also known as bubble stone, the main role is to refine the air from the oxygen pump, so that oxygen is easily dissolved in water.
[0057] Test Example 1
[0058] The indicators of the aquaculture water body of Example 1 were measured by a multifunctional water quality analyzer, including dissolved oxygen, pH; the settling volume of biological floc within 15 min was measured by an Imhoff cone, recorded as settling material (FV-15). The test results are shown in Table 1.
[0059] Table 1 Results of water quality indicators of the aquaculture water body of the oxygenation method of Example 1
[0060]
[0061]
[0062] Test Example 2
[0063] A blank control example was set. The blank control example artificially stirred the water body in the pool body to increase the dissolved oxygen at regular intervals, and stirred once every 6 hours every day, each time for 30 minutes.
[0064] At the beginning of the test, the blank control example and Example 2 both contained equal amounts of biological floc and equal numbers of Oriental red pufferfish, with an average of 500 grams of biological floc per kiloliter and 20 Oriental red pufferfish per kiloliter of water body. The initial water quality of the aquaculture water body of the blank control example and Example 2 was the same. The test lasted for 6 days, and the results of the biological floc activity and the aquaculture product activity of the blank control example and Example 2 were recorded on the first day, the second day, the third day, and the sixth day, as shown in Table 2.
[0065] Table 2 Comparison results of Example 2 and the blank control example
[0066]
[0067]
[0068] Although the present application has been described in detail in the foregoing general description, specific embodiments and tests, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present application. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application are within the scope of the present application.
Claims
1. A method for setting up a biological floc cultivation environment, characterized in that: The steps include: A cement pool measuring 6m x 6m square was set up. Eight sections of first aeration pipes, each 1.75m long and 0.8-1.6cm in diameter, were fixed to the bottom wall of the pool, 0.5m away from the inner wall. Both ends of the first aeration pipes were fitted with plugs, and the pipes were connected to the air pump outlet via a three-way valve. A second aeration pipe, forming a ring with a radius of 0.4m, was installed in the center of the bottom of the pool. The two ends of the second aeration pipe were fixedly connected via a three-way valve. The three-way valve was also connected to the air pump outlet, which was used to fill the pool with air. The first and second aeration pipes were microporous nano-aeration pipes. After starting the air pump to fill the tank with gas, the air encounters the inner wall and flows back and forth, causing the water in the pool to roll and stir in one direction. When the biological flocs rotate to the center with the water, the annular aeration pipe located at the center of the bottom of the pool is inflated to drive the water to roll. The upward movement of the gas causes the water to flow to the surface and roll to both sides. The gas on the side close to the inner wall of the pool encounters the force of the inner wall and rotates and rolls again to form a backwash water flow.
Citation Information
Patent Citations
Litopenaeus vannamei outdoor culture device with aeration nanotubes connected with PVC (polyvinyl chloride) tubes
CN103535316A
External litopenaeus vannamei recirculating aquaculture system
CN110192538A
Homogeneous aeration tank
CN2390876Y