Method for improving drought tolerance and promoting seed germination of elymus nutans or e. nutans

Abstract

The application discloses a method for improving drought resistance and promoting seed germination of elytraria acida or agropyron mongolicum, and relates to the field of plant drought stress. The elytraria acida seeds to be treated are mixed with corn cob particles with plant hormones or with auxin analogs and coating agents according to a mass ratio of 1-2:100:0.2 to obtain elytraria acida seed packages. The inventors use the idea of slowly releasing low-concentration plant hormones or auxin analogs, and set low-concentration plant hormones or auxin analogs in the seed coating in advance. The seed packages slowly release low-concentration plant hormones or auxin analogs after shallowly burying in the environment to absorb water, which can help the elytraria acida seeds germinate in a drought environment. Especially in an extremely dry environment, the drought resistance of the elytraria acida is significantly improved. In addition, a method for improving the drought resistance of agropyron mongolicum is provided, and a suitable range of naphthaleneacetic acid is blended with seed coating agents to prepare agropyron mongolicum seed packages.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant drought stress, in particular, to a method for improving drought tolerance and promoting seed germination of Elymus dahuricus or Agropyron mongolicum. BACKGROUND

[0002] Elymus dahuricus is a perennial bunchgrass of Poaceae, mainly distributed in the cold temperate zone of the northern hemisphere, and the main distribution areas are northwest, north China, northeast and southwest China. Elymus dahuricus has the characteristics of drought resistance, wind and sand resistance, salt and alkali tolerance, and developed root system, which can absorb more water. Elymus dahuricus has high yield, rich in crude protein, high nutritional value and high forage value. The leaf has a special xerophytic structure, which can curl in a dry environment, effectively reducing water loss and maintaining its growth. Elymus dahuricus is not only the main component of grassland and meadow, but also maintains the ecological balance of the region, and plays an important role in water and soil conservation, ecological restoration, grassland construction and development of animal husbandry.

[0003] Agropyron mongolicum, also known as sand grass, is a perennial herb of Poaceae, widely distributed in the temperate zone of Eurasia. In China, it is mainly distributed in the sandy habitats of dry grassland and desert grassland in northwest and north China, and can adapt to the environment of poor sandy soil, has good forage palatability, and is an important ecological grass species for improving grassland and increasing vegetation coverage and grass yield in the restoration and reconstruction of desert grassland in the north.

[0004] At present, although Elymus dahuricus or Agropyron mongolicum has the characteristics of drought resistance and wind and sand resistance, the seed germination rate is still low when it is used for grassland reseeding and planting due to the influence of drought environment stress.

[0005] Therefore, the present application is proposed. SUMMARY

[0006] The purpose of the present application is to provide a method for improving drought tolerance and promoting seed germination of Elymus dahuricus or Agropyron mongolicum, thereby improving the germination rate of seeds.

[0007] The inventor found that the reason for low seed germination rate is that traditional sowing, aerial seeding and reseeding planting are often accompanied by watering to promote seed germination, but in dry and semi-dry environment, the climate is dry and the evaporation amount is large, the water evaporates quickly, the water is consumed before the seed germinates, and the seed is stressed by drought, thereby resulting in low overall germination rate.

[0008] The present application is realized as follows:

[0009] In a first aspect, the present application provides a method for improving drought tolerance of Elymus nutans, comprising the following steps: mixing seeds to be treated with porous materials, coating agents with plant hormones or with auxin analogues according to a mass ratio of 1-2:100:0.2 to obtain Elymus nutans seed packets; the plant hormones are selected from gibberellins, and the auxin analogues are selected from naphthalene acetic acid;

[0010] The porous materials are selected from corn cobs, coconut coir or matrix soil.

[0011] The porous materials with plant hormones are prepared by immersing the porous materials in a solution of naphthalene acetic acid at a concentration of 0.3-0.9 mg / L.

[0012] The porous materials with auxin analogues are prepared by immersing the porous materials in a solution of gibberellins at a concentration of 50-250 mg / L.

[0013] The inventors have found through long-term experimental research that even if seeds are treated by seed soaking with plant hormones or auxin analogues, the seed germination is easily inhibited, and the effect is opposite, i.e., the seed germination rate is low. In order to completely change the problem of low seed germination rate of highland drought-tolerant plant seeds, the inventors use the idea of slow release of low-concentration plant hormones or auxin analogues, and set low-concentration plant hormones or auxin analogues in the seed coating in advance. After the seed packet is used by shallow burying, a small amount of water can be absorbed in the environment, and after water absorption, the seed packet slowly releases low-concentration gibberellins, which can help the Elymus nutans seeds germinate in a dry environment. By mixing the seeds, the porous materials and the coating agents according to a specific mass ratio, the Elymus nutans seed packets can be obtained, which can make the Elymus nutans have higher drought tolerance, especially in an extremely dry environment, compared with uncoated seeds, the drought tolerance of the Elymus nutans is significantly improved. In addition, the seed packet is not easily blown away by the wind after being formed into a lump, and the utilization rate of the seeds for sowing is improved.

[0014] In other embodiments, the porous materials can also be other porous loose wood materials.

[0015] In a preferred embodiment of the present application, the porous materials with plant hormones are prepared by immersing the porous materials in a solution of naphthalene acetic acid at a concentration of 0.3-0.9 mg / L. Immersing the porous materials at the above concentration helps the seed packet to slowly release low-concentration naphthalene acetic acid after water absorption, which promotes the germination of Elymus nutans seeds. If the concentration is too high, the germination of Elymus nutans seeds will be inhibited to a certain extent. Below the above concentration range, the effect of improving the drought tolerance of Elymus nutans is limited.

[0016] The concentration of the naphthalene acetic acid solution includes but is not limited to 0.6 mg / L, 0.8 mg / L, 0.9 mg / L, 1 mg / L, 1.1 mg / L or 1.2 mg / L.

[0017] 0.6-1.2 mg / L concentration of naphthalene acetic acid can significantly improve the germination of Puccinellia distans seeds under extreme drought stress (-1.5 Mpa < environmental osmotic potential < -1 Mpa), and the germination rate of Puccinellia distans seeds is increased by about 14.3%.

[0018] In an alternative embodiment, the soaking is 6-24 hours, and the soaking is removed and drained.

[0019] In a preferred embodiment of the application, the porous material with auxin analogues is prepared by soaking the porous material in a gibberellin solution at a concentration of 450-250 mg / L. At the above concentration, the germination rate of Puccinellia distans seeds under environmental osmotic potential -1.4 Mpa to -1 (severe) drought stress can be increased by 27%-67.5%, and the drought tolerance of Puccinellia distans can be improved.

[0020] For example, the porous material is soaked in a gibberellin solution at a concentration of 50-150 mg / L, or 100-200 mg / L, or 150-250 mg / L.

[0021] The inventor has proven that when the gibberellin concentration is 50 mg / L≤250 mg / L, the germination rate of Puccinellia distans seeds under environmental osmotic potential -1.4 Mpa to -1 (severe) drought stress can be increased by 31.02%-80.51%.

[0022] In an alternative embodiment, the soaking is 6-24 hours, and the soaking is removed and drained.

[0023] In an alternative embodiment, the soaking is 12 hours.

[0024] In a preferred embodiment of the application, the Puccinellia distans seeds to be treated are mixed with the porous material with plant hormones or auxin analogues and the coating agent, and then stirred.

[0025] In an alternative embodiment, the stirring temperature is less than 10°C; to avoid the seeds from triggering premature germination under suitable temperature conditions when encountering water, resulting in a decrease in the germination rate of the seeds after being sown.

[0026] In an alternative embodiment, the stirring temperature is 5-8°C. For example, 5°C, 6°C, 7°C, or 8°C.

[0027] The mixing of the Puccinellia distans seeds to be treated with the porous material with plant hormones or auxin analogues and the coating agent, as well as the stirring process, are all carried out in complete darkness. The seed germination effect is better using complete darkness.

[0028] In an alternative embodiment, the coating agent is selected from at least one of a water-retaining agent, an anti-freezing agent, an osmotic agent, a film-forming agent, a binding agent, and a filling agent.

[0029] In an alternative embodiment, the water-retaining agent is carboxymethyl cellulose.

[0030] In an alternative embodiment, the anti-freezing agent is glycerol.

[0031] In an alternative embodiment, the osmotic agent is a water-soluble thioketone.

[0032] In an alternative embodiment, the film-forming agent is chitosan.

[0033] In an alternative embodiment, the binding agent is selected from at least one of a styrene-acrylic emulsion and carboxymethyl cellulose.

[0034] In an alternative embodiment, the filling agent includes at least one of bentonite and talcum powder.

[0035] In a second aspect, the present application further provides a method for promoting germination of Puccinellia distans seeds, wherein the Puccinellia distans seeds obtained by the method for improving drought tolerance of Puccinellia distans seeds are cultured under the following conditions: complete darkness and constant temperature of 15-30°C; or light and dark alternation (12h light / 12h dark) and constant temperature of 15-20°C.

[0036] The inventors have found that the seeds of Puccinellia distans have better germination effect under the above conditions. In an embodiment, the seeds cultured under the above conditions can be transplanted to a field after germination.

[0037] In a third aspect, the present application further provides a method for promoting germination of Puccinellia distans seeds under extremely dry conditions, wherein the Puccinellia distans seeds obtained by the method for improving drought tolerance of Puccinellia distans seeds are embedded in the surface layer of soil, and if the range of the environmental osmotic potential measured in the field is -1.5MPa

[0038] or if the range of the environmental osmotic potential measured in the field is -1.5MPa

[0039] Preferably, if the range of the environmental osmotic potential measured in the field is -1.2MPa

[0040] The seed package is used by shallow burying, and only a small amount of water is absorbed in an extremely dry environment. After absorbing water, the seed package slowly releases low-concentration gibberellins, which can help the seed germination of the elymus dahuricus in a dry environment and improve the germination rate. Before planting, the environmental osmotic potential is measured on site, and the appropriate elymus dahuricus seed package is selected according to the environmental osmotic potential of different planting sites. After screening and verification, the inventor finds that the elymus dahuricus seed package with gibberellins has a better effect of greatly improving the germination rate of the elymus dahuricus seed, and the improvement is 27% to 80.51%.

[0041] In a fourth aspect, the application also provides a method for improving the drought resistance of elymus dahuricus, which comprises: mixing the elymus dahuricus seeds to be treated with a porous material with a growth hormone analogue and a coating agent according to a mass ratio of 1-2:100:0.2 to obtain an elymus dahuricus seed package; and the growth hormone analogue is selected from naphthalene acetic acid.

[0042] By preparing a seed coating for the elymus dahuricus seed, the drought resistance of the elymus dahuricus can be improved, and the seed germination rate of the elymus dahuricus under dry conditions is greatly improved. The inventor finds that the required naphthalene acetic acid concentration of the elymus dahuricus seed is extremely low and difficult to control, and exceeding the appropriate range will inhibit the seed germination. The inventor can achieve the effect of promoting the seed germination of the elymus dahuricus by preparing a seed coating for the elymus dahuricus, which slowly releases low-concentration naphthalene acetic acid.

[0043] The seed package can absorb a small amount of water in the environment after being used by shallow burying, and slowly releases low-concentration naphthalene acetic acid after absorbing water, which can help the seed germination of the elymus dahuricus in a dry environment.

[0044] The porous material is easy to obtain, the method for improving the drought resistance of the elymus dahuricus is simple and easy to implement, and the elymus dahuricus seed package is less affected by the environment.

[0045] In a preferred embodiment of the application, the porous material with the growth hormone analogue is prepared by immersing the porous material in a solution of 0.3-1.2 mg / L naphthalene acetic acid.

[0046] When the naphthalene acetic acid concentration is 0.3 mg / L to 0.6 mg / L, the germination rate of the elymus dahuricus seed under the environmental osmotic potential of 0 to -0.5 Mpa (mild) drought stress can be improved by 10% to 27%;

[0047] When the naphthalene acetic acid concentration is 0.6 mg / L to 1.2 mg / L, the germination rate of the elymus dahuricus seed under the environmental osmotic potential of -0.5 to -1.1 Mpa (severe) drought stress can be improved by 3% to 30%.

[0048] In an alternative embodiment, the immersion time is 6-24 hours, and the porous material is taken out and drained.

[0049] In an alternative embodiment, the seeds are soaked for 12 hours.

[0050] In an alternative embodiment, the seeds to be treated are mixed with the porous material with the auxin analog, the coating agent, and further comprising stirring.

[0051] In an alternative embodiment, the stirring temperature is less than 10℃; avoiding the seeds from triggering early germination when exposed to water at suitable temperature, resulting in a decrease in the germination rate of the seeds after being sown.

[0052] In an alternative embodiment, the stirring temperature is 5-8℃. For example, the stirring temperature is 5℃, 6℃, 7℃, or 8℃.

[0053] In a fifth aspect, the present application further provides a method for promoting the germination of the seeds of Mongolian ice grass, wherein the seeds of Mongolian ice grass obtained by the method for improving the drought tolerance of Mongolian ice grass are cultured under the following conditions: complete darkness, constant temperature 15-25℃; or light and dark alternation (12h light / 12h dark), constant temperature 15-25℃.

[0054] The inventors have found that the seeds of Mongolian ice grass cultured under the condition of light and dark alternation (12h light / 12h dark), constant temperature 15-25℃ have the best germination effect, and the lengths of the radicle and plumule are longer.

[0055] The constant temperature is, for example, 15℃, 18℃, 21℃, 22℃, 23℃, 24℃, or 25℃.

[0056] In a sixth aspect, the present application further provides a method for promoting the germination of the seeds of Mongolian ice grass under drought conditions, wherein the seeds of Mongolian ice grass obtained by the method for improving the drought tolerance of Mongolian ice grass are embedded in the surface layer of soil, and if the environmental osmotic potential measured in the field is in the range of -0.5MPa≤environmental osmotic potential<0MPa, the seeds of Mongolian ice grass with naphthalene acetic acid are selected; the porous material with naphthalene acetic acid is prepared by the following method: the porous material is soaked in a solution of naphthalene acetic acid with a concentration of 0.3mg / L≤naphthalene acetic acid concentration≤0.6mg / L; and then the porous material with naphthalene acetic acid is used to prepare the seeds of Mongolian ice grass with naphthalene acetic acid. When -0.5MPa≤environmental osmotic potential≤-0.2Mpa, the use of 0.6mg / L naphthalene acetic acid concentration can increase the germination rate by about 13.4%; when -0.2Mpa<environmental osmotic potential<0Mpa, the use of 0.3mg / L naphthalene acetic acid concentration can increase the germination rate by about 18.3%.

[0057] If the range of the measured environmental osmotic potential in the field is -1.1 MPa < environmental osmotic potential < -0.5 MPa, the seed package of Agropyron mongolicum with naphthalene acetic acid is selected; the porous material with naphthalene acetic acid is prepared by the following method: the porous material is soaked in a solution of naphthalene acetic acid with a concentration of 0.6 mg / L < naphthalene acetic acid concentration < 1.2 mg / L; and then the porous material with naphthalene acetic acid is used to prepare the seed package of Agropyron mongolicum with naphthalene acetic acid.

[0058] In actual sowing, the environmental osmotic potential in the field is measured first, and then the porous material is soaked in a naphthalene acetic acid solution with a suitable concentration according to the environmental osmotic potential, and then the corresponding seed package is prepared.

[0059] The present application has the following beneficial effects:

[0060] (1) The inventor adopts the idea of slow release of low-concentration plant hormones or auxin analogs, and sets low-concentration plant hormones or auxin analogs in the seed coating in advance. After the seed package is used by shallow burying, a small amount of water can be absorbed in the environment, and after water absorption, the seed package slowly releases low-concentration plant hormones or auxin analogs, which can help the seed germination of Agropyron cristatum in a drought environment. The seed, porous material and coating agent are mixed in a specific mass ratio to obtain the seed package of Agropyron cristatum, which can make Agropyron cristatum have higher drought resistance, especially in extremely dry environments, compared with uncoated seeds, the drought resistance of Agropyron cristatum is significantly improved.

[0061] (2) The seed package of the inventor is not easy to be blown away in the environment after being baled, which improves the utilization rate of sown seeds.

[0062] (3) A method for promoting seed germination of Agropyron cristatum and seed germination of Agropyron cristatum under extremely dry conditions is also provided. The method can select appropriate seed packages of Agropyron cristatum according to the environmental osmotic potential of the sowing site, and adapt to the local conditions.

[0063] (4) The inventor also provides a method for improving the drought resistance of Agropyron mongolicum, which adopts a suitable range of naphthalene acetic acid and seed coating agent to blend and prepare a seed package of Agropyron mongolicum. After the seed package is used by shallow burying, a small amount of water can be absorbed in the environment, and after water absorption, the seed package slowly releases low-concentration auxin analogs, which can help the seed germination of Agropyron mongolicum in a drought environment.

[0064] (5) A method for promoting seed germination of Agropyron mongolicum and a method for promoting seed germination of Agropyron mongolicum under dry conditions are also provided, which can help significantly improve the germination rate of Agropyron mongolicum seeds, especially under dry conditions. BRIEF DESCRIPTION OF DRAWINGS

[0065] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some of the embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0066] Figure 1 The germination rate of Agrostis stolonifera seeds under different temperature and light conditions;

[0067] Figure 2 The experimental result graph of the influence of adding gibberellin on the germination of Agrostis stolonifera seeds in a drought environment;

[0068] Figure 3 The experimental result graph of the influence of adding different concentrations of naphthalene acetic acid on the germination of Agrostis stolonifera seeds in a drought environment;

[0069] Figure 4 The germination rate of Agrostis stolonifera seeds under different temperature and light conditions;

[0070] Figure 5 The experimental result graph of the influence of adding different concentrations of naphthalene acetic acid on the germination of Agrostis stolonifera seeds in a drought environment;

[0071] Figure 6 The experimental result graph of the influence of adding different concentrations of naphthalene acetic acid on the germination of Agrostis stolonifera seeds in a drought environment;

[0072] Figure 7 The experimental result graph of the germination rate of Agrostis stolonifera seeds after the seed coating prepared by different concentrations of naphthalene acetic acid or gibberellin is sown under different environmental precipitation conditions;

[0073] Figure 8 The experimental result graph of the germination rate of Agrostis stolonifera seeds after the seed coating prepared by different concentrations of naphthalene acetic acid or gibberellin is sown under different environmental precipitation conditions. DETAILED DESCRIPTION

[0074] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below. If the specific conditions are not indicated in the embodiments, the conventional conditions or the conditions suggested by the manufacturer are adopted. If the reagents or instruments are not indicated by the manufacturer, they are all conventional products that can be purchased in the market.

[0075] The features and performances of the present application will be further described in detail below in combination with the embodiments. (I)

[0077] Embodiment 1

[0078] The embodiment provides a method for improving drought resistance of Elymus nutans.

[0079] 1. The corn cob is made into powder, and the powder particles are single block particles or spherical particles with a diameter of about 2-3 mm;

[0080] 2. The crushed corn cob particles are sprayed with 0.5% (w / v) sodium hypochlorite solution or 75% (w / v) alcohol for sterilization and disinfection treatment;

[0081] 3. The sterilized corn cob particles are sprayed with sterile distilled water for washing, and the sterilization residual liquid is washed away, and the washing process is repeated about 3-5 times according to the sterilization condition;

[0082] 4. The sterilized and washed corn cob particles are placed in an oven for drying;

[0083] 5. The dried corn cob particles are soaked in a naphthalene acetic acid solution with a concentration of 0.6 mg / L for 24 hours, and then taken out and drained;

[0084] 6. The Elymus nutans seeds with uniform size and full grains are selected, and 0.05% sodium hypochlorite solution is sprayed for disinfection; the sodium hypochlorite solution is sprayed for 2-3 seconds, and then the seeds are washed with distilled water with a temperature of 5-8℃;

[0085] 7. The disinfected Elymus nutans seeds, the corn cob particles treated in step 5 and carboxymethyl cellulose are stirred and mixed, and the mixing mass ratio is 1:100:0.2, and the stirring temperature is controlled to be 8℃;

[0086] 8. The mixed material in step 7 is pressed into a block, and the length, width and height of the block are about 10-15 cm, and the block can be a cube or a cuboid, that is, the Elymus nutans seed bag.

[0087] In addition, the embodiment also provides a method for promoting germination of Elymus nutans seeds.

[0088] The Elymus nutans seed bag is cultured to germination in complete darkness and at a constant temperature of 20℃, and then is transplanted to a field for culture.

[0089] Embodiment 2

[0090] The embodiment provides a method for improving drought resistance of Elymus nutans, and the difference from the embodiment 1 is only that step 5 is different, and the other steps are the same.

[0091] 5. The dried corn cob particles are soaked in a naphthalene acetic acid solution with a concentration of 0.9 mg / L for 24 hours, and then taken out and drained.

[0092] Embodiment 3

[0093] The difference compared with Example 1 is only that step 5 is different, and the other steps are the same.

[0094] 5. The dried corn cob particles are soaked in a naphthaleneacetic acid solution with a concentration of 1.2 mg / L, and soaked for 24 hours, and then drained.

[0095] Example 4

[0096] The present example provides a method for improving the drought tolerance of Agropyron cristatum, which uses gibberellin and corn cob particles for blending, and the specific steps are as follows:

[0097] 1. Corn cob is made into powder, and the powder particles can be square or spherical particles with a particle size of about 2-3 mm in diameter;

[0098] 2. The crushed corn cob particles are sprayed with 0.5% (w / v) sodium hypochlorite solution or 75% (w / v) alcohol for disinfection and sterilization treatment;

[0099] 3. The sterilized corn cob particles are rinsed with sterile distilled water, and the disinfection residue is washed off, and the rinsing process is repeated about 3-5 times depending on the disinfection situation;

[0100] 4. The sterilized and rinsed corn cob particles are placed in an oven for drying;

[0101] 5. The dried corn cob particles are soaked in a gibberellin solution with a concentration of 50 mg / L, soaked for 24 hours, and then drained;

[0102] 6. Select Agropyron cristatum seeds with uniform size and full grains, and use 0.05% sodium hypochlorite solution for disinfection, and rinse with distilled water at a temperature of 5-8°C after the sodium hypochlorite solution is sprayed for 2-3 seconds;

[0103] 7. Agropyron cristatum seeds, corn cob particles treated in step 5, and carboxymethyl cellulose are mixed by stirring, with a mass ratio of 1:100:0.2, and the stirring temperature is controlled at 8°C;

[0104] 8. The mixed material in step 7 is pressed into a block, which can be a cube or a cuboid with a length, width, and height of about 10-15 cm, which is an Agropyron cristatum seed package.

[0105] Example 5

[0106] The present example provides a method for improving the drought tolerance of Agropyron cristatum, which uses gibberellin and corn cob particles for blending, and the difference compared with Example 4 is only that step 5 is different, and the other steps are the same.

[0107] 5. The dried corn cob particles are soaked in a gibberellin solution with a concentration of 100 mg / L, soaked for 24 hours, and then drained.

[0108] Example 6

[0109] The present example provides a method for improving the drought tolerance of Elymus nutans, which uses gibberellin and corn cob particles for blending. Compared with Example 4, the only difference is that step 5 is different, and the remaining steps are the same.

[0110] 5. The dried corn cob particles are soaked in a solution of gibberellin with a concentration of 150 mg / L, and soaked for 24 hours. Then, the particles are taken out and drained.

[0111] Example 7

[0112] The present example provides a method for improving the drought tolerance of Elymus nutans, which uses gibberellin and corn cob particles for blending. Compared with Example 4, the only difference is that step 5 is different, and the remaining steps are the same.

[0113] 5. The dried corn cob particles are soaked in a solution of gibberellin with a concentration of 200 mg / L, and soaked for 24 hours. Then, the particles are taken out and drained.

[0114] Example 8

[0115] The present example provides a method for improving the drought tolerance of Elymus nutans, which uses gibberellin and corn cob particles for blending. Compared with Example 4, the only difference is that step 5 is different, and the remaining steps are the same.

[0116] 5. The dried corn cob particles are soaked in a solution of gibberellin with a concentration of 250 mg / L, and soaked for 24 hours. Then, the particles are taken out and drained.

[0117] Example 9

[0118] The present example provides a method for promoting the germination of Elymus nutans seeds under extremely dry conditions, which embeds the Elymus nutans seeds obtained from any one of Examples 1-8 above into the surface layer of soil.

[0119] If the range of the measured environmental osmotic potential is -1.5 MPa < environmental osmotic potential < -1 MPa, the Elymus nutans seed package with gibberellin (Examples 4-8) is selected.

[0120] Or if the range of the measured environmental osmotic potential is -1.5 MPa < environmental osmotic potential < -1.1 MPa, the Elymus nutans seed package with naphthalene acetic acid (Examples 1-3) is selected, preferably the Elymus nutans seed package with gibberellin.

[0121] In an alternative embodiment, if the range of the measured environmental osmotic potential is -1.2 MPa < environmental osmotic potential < -1 MPa, the Elymus nutans seed package with gibberellin is selected.

[0122] Comparative Example 1

[0123] The difference compared with Example 1 is only that step 5 is different, and the rest of the steps are the same.

[0124] 5, the dried corn cob particles are placed in water without auxin analogues and without plant hormones, soaked for 24 h, taken out and drained.

[0125] Comparative Example 2

[0126] The difference compared with Example 1 is only that step 5 is different, and the rest of the steps are the same.

[0127] 5, the dried corn cob particles are placed in a naphthalene acetic acid solution with a concentration of 0.3 mg / L, soaked for 24 h, taken out and drained.

[0128] Comparative Example 3

[0129] The difference compared with Example 1 is only that step 5 is different, and the rest of the steps are the same.

[0130] 5, the dried corn cob particles are placed in a naphthalene acetic acid solution with a concentration of 1.5 mg / L, soaked for 24 h, taken out and drained.

[0131] Comparative Example 4

[0132] The difference compared with Example 4 is only that step 5 is different, and the rest of the steps are the same.

[0133] 5, the dried corn cob particles are placed in a gibberellin solution with a concentration of 20 mg / L, soaked for 24 h, taken out and drained.

[0134] Comparative Example 5

[0135] The difference compared with Example 4 is only that step 5 is different, and the rest of the steps are the same.

[0136] 5, the dried corn cob particles are placed in a gibberellin solution with a concentration of 300 mg / L, soaked for 24 h, taken out and drained.

[0137] Experimental Example 1

[0138] This experimental example aims to explore the optimal temperature and light of Agrostis stolonifera, in order to clarify the optimal light and temperature conditions suitable for the germination of Agrostis stolonifera seeds.

[0139] In the artificial intelligence climate incubator, light and dark alternation (12 h light / 12 h dark) and continuous darkness (24 h dark) are set as two light conditions; 15 / 5, 20 / 10, 25 / 15, 30 / 20℃, four light and dark temperature treatment conditions and six constant temperature treatments (5, 10, 15, 20, 25, 30℃) are set.

[0140] Procedure: Sterilized seeds of Agrostis stolonifera were evenly placed in 90mm Petri dishes with double layers of filter paper, 25 seeds per dish, and distilled water was added. Four replicates were set up for each treatment. The number of seeds germinating normally was recorded every day during the experiment, and the filter paper was kept moist by adding distilled water as needed. The radicle length was more than half the length of the seed was determined to be germination, and the germinated seeds were removed from the Petri dishes. The test lasted for 14 days, and the germination rate was calculated on the 5th day and the germination rate was calculated on the 14th day. The Petri dishes for continuous dark treatment were wrapped with tin foil to block light, and the seed germination statistics were completed quickly under weak light.

[0141] Results are shown in the table below Figure 1 The optimal results were: alternating light and dark + constant temperature 20°C, the highest germination rate was about 95±5.0%, constant temperature 25°C + alternating light and dark 12 hours, the longest germination radicle was 3.95±0.46 cm, and constant temperature 20°C + alternating light and dark, the longest radicle was 6.55±0.37 cm.

[0142] The best seed germination effect was obtained by using complete darkness and constant temperature 20-30°C for Agrostis stolonifera, so the seed package should be completely shaded during the production process, and the seed sterilization and stirring process should be carried out in an environment below 10°C to avoid premature germination of the seeds under suitable temperature conditions when they come into contact with water, resulting in a decrease in the germination rate after sowing the seed package. The difference in seed germination rate was not significant under light and dark temperature treatment, and the temperature range was 20-30°C.

[0143] As shown in Comparative Examples 4 and 5, concentrations exceeding 250 mg / L inhibited seed germination, and concentrations below 50 mg / L had little effect compared to the water group.

[0144] Experimental Example 2

[0145] The suitable concentration of gibberellin that can stimulate the germination of Agrostis stolonifera seeds that have been subjected to drought stress was studied.

[0146] T0 (0 mg / L), G1 (50 mg / L), G2 (100 mg / L), G3 (150 mg / L), G4 (200 mg / L), and G5 (250 mg / L) were set up for Agrostis stolonifera seed treatment, and PEG solutions with osmotic potentials of 0 MPa, -0.3 MPa, -0.6 MPa, -0.9 MPa, -1.2 MPa, and -1.5 MPa were configured under constant temperature 25°C to simulate different degrees of drought stress. The seeds were placed in 90mm Petri dishes with double layers of filter paper, 25 seeds per dish, and different concentrations of 8ml PEG-6000 solution was added. The Petri dishes were sealed with Parafilm, placed in a 25°C constant temperature artificial climate incubator, and subjected to germination test under light and dark alternation (12h light / 12h dark), with 3 replicates for each treatment.

[0147] Reference to experimental results Figure 2 As shown in the table, gibberellin has inhibitory effect on the seed germination of Puccinellia distans under non-stress or mild drought stress environment. When extreme drought occurs (-1 Mpa < environmental osmotic potential < -1.5 Mpa), 50-250 mg / L concentration of gibberellin can promote the seed germination of Puccinellia distans, and the germination rate is increased from 0 to about 82.3%.

[0148] Experimental Example 3

[0149] Different concentrations of naphthalene acetic acid (T0 (0 mg / L), G1 (0.3 mg / L), G2 (0.6 mg / L), G3 (0.9 mg / L), G4 (1.2 mg / L), G5 (1.5 mg / L)) were used to soak Puccinellia distans seeds for 12 hours, and PEG solutions with osmotic potentials of 0 MPa, -0.3 MPa, -0.6 MPa, -0.9 MPa, -1.2 MPa, and -1.5 MPa at a constant temperature of 20°C were used to simulate different degrees of drought stress. The soaked pasture seeds were placed in 90 mm culture dishes lined with double layers of filter paper, 8 ml of PEG-6000 solution with different concentrations was added, the culture dishes were sealed with Parafilm sealing film, and the germination test was carried out in a constant temperature artificial climate incubator at 25°C under complete darkness. Each treatment was set up with 3 replicates.

[0150] Reference to experimental results Figure 3 As shown in the table, the results show that 0.3-0.9 mg / L concentration of naphthalene acetic acid can significantly improve the seed germination of Puccinellia distans under extreme drought stress (-1 Mpa < environmental osmotic potential < -1.5 Mpa). The improvement effect is about 14.3%.

[0151] Experimental Example 4

[0152] Effect of seed coating prepared by adding gibberellin and naphthalene acetic acid on the germination rate of Puccinellia distans seeds.

[0153] Experimental method:

[0154] 1. Corn cob powder was prepared, and the powder particles were square particles with a diameter of about 2-3 mm;

[0155] 2. The crushed corn cob particles were sprayed with 75% (w / v) alcohol for disinfection and sterilization treatment;

[0156] 3. The sterilized corn cob particles were rinsed with sterile distilled water, and the disinfection residue was washed off. The rinsing process was repeated about 3-5 times depending on the disinfection situation;

[0157] 4. The sterilized and rinsed corn cob particles were placed in an oven for drying;

[0158] 5, the dried corncob particles are respectively placed in gibberellin solutions with concentrations of 0.3, 0.6, 0.9, 1.2, 1.5 mg / L naphthalene acetic acid (N1-N5) and 50, 100, 150, 200, 250 mg / L (G1-G5), and soaked for 24 hours, and then taken out and drained;

[0159] 6, select uniform size, full grain Puccinellia pezeris seeds, and spray disinfect with 0.05% sodium hypochlorite solution, and rinse clean with distilled water at a temperature of 5-8°C after spraying with the sodium hypochlorite solution for 2-3 seconds;

[0160] 7, mix the disinfected Puccinellia pezeris seeds, the corncob particles treated in step 5, and carboxymethyl cellulose, with a mixing mass ratio of 1:100:0.2, and control the stirring temperature at 8°C;

[0161] 8, press the mixture obtained in step 7 into blocks, and make Puccinellia pezeris seed packets in the shape of cubes with a length, width, and height of about 10-15 cm, each seed packet containing about 50 Puccinellia pezeris seeds.

[0162] 9, place the seed packets into a greenhouse with an environmental temperature of 25°C, and plant them in flowerpots for cultivation, with one seed packet planted in each flowerpot (diameter 50 cm), simulate the annual precipitation environment in arid regions, and set the gradient precipitation from wet to dry as P1 (150%), P2 (125%), P3 (100%), P4 (75%), and P5 (50%) times the environmental precipitation (about 185 mm in the central arid region of Ningxia), and perform seed water replenishment. Observe the seed germination rate after one growth cycle;

[0163] Results are shown in the table Figure 7 As shown in the table, the results show that under the P1 (150%) environmental precipitation condition, the seed packets soaked in gibberellin (G1-G4) can slightly increase the germination of Puccinellia pezeris, and the germination rate of the Puccinellia pezeris seed packets soaked in naphthalene acetic acid with concentrations of N3-N5 and G5 gibberellin concentration shows a significant inhibitory effect;

[0164] 2: Under the P2 (125%) and P3 (100%) environmental precipitation conditions, the Puccinellia pezeris seed packets soaked in gibberellin and naphthalene acetic acid all show an inhibitory effect on germination;

[0165] 3: Gibberellin, naphthalene acetic acid soaking after the seed package germination rate of Agrostis stolonifera is higher than the water treatment group under the condition of P4 (75%) and P5 (50%) environmental precipitation, P4 environmental precipitation conditions, G1-G5 concentration of gibberellin soaking can improve the germination rate of Agrostis stolonifera about 3%-34.3%; N1-N5 concentration of naphthalene acetic acid soaking can improve the germination rate of Agrostis stolonifera about 2.3%-12%; P5 environmental precipitation conditions, G1-G5 gibberellin soaking can improve the germination rate of Agrostis stolonifera about 19.1%-45.3%; N1-N4 gibberellin soaking can improve the germination rate of Agrostis stolonifera about 6%-13.3%;

[0166] 4: P3, P4 drought conditions, G3-G4 concentration of gibberellin; N2-N3 concentration of naphthalene acetic acid soaking after the seed package germination rate is higher. (II)

[0168] Example 1

[0169] The embodiment provides a method for improving drought resistance of Agrostis stolonifera, and the specific steps are as follows:

[0170] 1. The corn cob is made into powder, and the powder particles are single block particles with a size of about 2-3mm in diameter.

[0171] 2. The crushed corn cob particles are sprayed with 0.5% (w / v) sodium hypochlorite solution or 75% (w / v) alcohol for disinfection and sterilization treatment.

[0172] 3. The sterilized corn cob particles are sprayed with sterile distilled water for rinsing, and the disinfection residue is rinsed off. The rinsing process is repeated about 3-5 times according to the disinfection condition.

[0173] 4. The sterilized and rinsed corn cob particles are placed in an oven for drying.

[0174] 5. The dried corn cob particles are soaked in a 0.3mg / L naphthalene acetic acid solution for 24 hours, and then drained.

[0175] 6. Select uniform size and full grain Agrostis stolonifera seeds, and use 0.05% sodium hypochlorite solution for disinfection. After 2-3 seconds of contact with the sodium hypochlorite solution, rinse with distilled water at a temperature of 5-8℃.

[0176] 7. Mix the disinfected Agrostis stolonifera seeds, the corn cob particles treated in step 5, and carboxymethyl cellulose by stirring, and the mixing mass ratio is 1:100:0.2. The stirring temperature is controlled at 8℃.

[0177] 8. The mixed material in step 7 is pressed into a block, which can be a cube or a cuboid with a length, width and height of about 10-15cm, i.e. an Agrostis stolonifera seed package.

[0178] Example 2

[0179] The embodiment provides a method for improving drought tolerance of Mongolian wheatgrass, and 0.6 mg / L naphthalene acetic acid is blended with corn cob particles, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0180] Example 3

[0181] The embodiment provides a method for improving drought tolerance of Mongolian wheatgrass, and 0.9 mg / L naphthalene acetic acid is blended with corn cob particles, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0182] Example 4

[0183] The embodiment provides a method for improving drought tolerance of Mongolian wheatgrass, and 1.2 mg / L naphthalene acetic acid is blended with corn cob particles, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0184] Example 5

[0185] The embodiment provides a method for improving drought tolerance of Mongolian wheatgrass, and 1.2 mg / L naphthalene acetic acid is blended with corn cob particles, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0186] Comparative example 1

[0187] The embodiment provides a method for improving drought tolerance of Mongolian wheatgrass, and 1.5 mg / L naphthalene acetic acid is blended with corn cob particles, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0188] Comparative examples 2-7

[0189] Comparative examples 2-7 provide a method for improving drought tolerance of Mongolian wheatgrass, and comparative examples 2-7 respectively use gibberellin T0 (0 mg / L), G1 (50 mg / L), G2 (100 mg / L), G3 (150 mg / L), G4 (200 mg / L), G5 (250 mg / L) and corn cob particles are blended, and compared with example 1, only step 5 is different, and the remaining steps are the same.

[0190] Experimental example 1

[0191] The embodiment is to explore the influence of temperature and light on seed germination, and to determine the optimum light and temperature conditions suitable for Mongolian wheatgrass seed germination.

[0192] Methods: The light and dark alternating (12h light / 12h dark) and continuous darkness (24h dark) two light conditions were set in the artificial intelligence climate incubator; 15 / 5, 20 / 10, 25 / 15, 30 / 20℃, four light and dark temperature treatment conditions and six constant temperature treatments (5, 10, 15, 20, 25, 30℃) were set.

[0193] Process: Sterilized pasture seeds were evenly placed in 90mm culture dishes with double-layer filter paper, and an appropriate amount of distilled water was added, with 4 replicates for each treatment. The number of normally germinated seeds was recorded every day during the experiment, and an appropriate amount of distilled water was added to keep the filter paper moist at all times. The radicle length exceeding half the length of the seed was determined as germination, and the germinated seeds were removed from the culture dish. The test lasted for 14 days, and the germination potential was calculated on the 5th day and the germination rate on the 14th day. The culture dishes for continuous darkness treatment were wrapped with tin foil to block light, and the seed germination statistics were completed quickly under weak light.

[0194] Results refer to Figure 4 The optimal results are as follows: light and dark alternating + constant temperature 20℃, the highest germination rate is about 95±5.0%, constant temperature 25℃+light and dark alternating 12 hours, the longest germination radicle is 3.95±0.46cm, constant temperature 20℃+light and dark alternating, the longest embryo bud length is 6.55±0.37cm.

[0195] The germination effect of Mongolian ice grass seeds cultured at constant temperature 20-25℃ with light and dark alternating (12h light / 12h dark) is the best, so there is no need to block light during the seed bag production process. The sterilization and stirring process of Mongolian ice grass seeds should be carried out in an environment of 5-8℃ to avoid premature germination of seeds triggered by water under suitable temperature conditions, resulting in a decrease in germination rate after sowing.

[0196] Experimental Example 2

[0197] This experimental example screened out the suitable concentration of naphthalene acetic acid that can stimulate the germination of Mongolian ice grass seeds that have been subjected to drought stress.

[0198] Different naphthalene acetic acid concentration (T0 (0 mg / L), G1 (0.3 mg / L), G2 (0.6 mg / L), G3 (0.9 mg / L), G4 (1.2 mg / L), G5 (1.5 mg / L)) was used to soak the grass seeds for 12 h, and the PEG solution with osmotic potential of 0 MPa, -0.3 MPa, -0.6 MPa, -0.9 MPa, -1.2 MPa, and -1.5 MPa at a constant temperature of 20 ℃ was used to simulate different degrees of drought stress. The soaked grass seeds were placed in 90 mm culture dishes with double layers of filter paper, 8 ml of PEG-6000 solution with different concentrations was added, the culture dishes were sealed with Parafilm sealing film, and the culture dishes were placed in a constant temperature artificial climate incubator at 20 ℃. The light and dark were alternated (12 h light / 12 h dark) to conduct the germination test. Each treatment was set with 3 replicates.

[0199] Results are shown in Figure 5 As shown in the results, when the naphthalene acetic acid concentration was 0.3 mg / L to 0.6 mg / L, the germination rate of the Mongolian ice grass seeds under the environmental osmotic potential of 0 to -0.5 Mpa (mild) drought stress was increased by 10% to 27%;

[0200] When the naphthalene acetic acid concentration was 0.6 mg / L to 1.2 mg / L, the germination rate of the Mongolian ice grass seeds under the environmental osmotic potential of -1.1 to -0.5 Mpa (severe) drought stress was increased by 3 to 30%. When the naphthalene acetic acid concentration was greater than 1.2 mg / L, the germination of the Mongolian ice grass seeds was inhibited.

[0201] Experimental Example 3

[0202] In this experimental example, the suitable concentration of gibberellin that can stimulate the germination of the Mongolian ice grass seeds that have suffered from drought stress was screened.

[0203] The steps were the same as those in Experimental Example 2, and gibberellin was added with concentrations of T0 (0 mg / L), G1 (50 mg / L), G2 (100 mg / L), G3 (150 mg / L), G4 (200 mg / L), and G5 (250 mg / L).

[0204] Results are shown in Figure 6 As shown in the results, it was found through the experiment that the gibberellin with concentrations of G1 to G5 had an inhibitory effect on the Mongolian ice grass seeds under different degrees of drought stress, and the germination rate of the seeds was reduced by about 6.7% to 29.3%. The higher the concentration of the added gibberellin, the more obvious the inhibitory effect on the Mongolian ice grass seeds.

[0205] Experimental Example 4

[0206] The effect of the seed coating prepared by adding gibberellin and naphthalene acetic acid on the germination rate of the Mongolian ice grass seeds.

[0207] Experimental method:

[0208] 1. Corn cob is made into powder, and the powder particles are single block particles with a size of about 2-3 mm in diameter;

[0209] 2. The crushed corn cob particles are sprayed with 75% (w / v) alcohol for sterilization and disinfection treatment;

[0210] 3. The sterilized corn cob particles are sprayed with sterile distilled water for rinsing, and the disinfection residue is rinsed out. The rinsing process is repeated about 3-5 times depending on the disinfection situation;

[0211] 4. The sterilized and rinsed corn cob particles are placed in an oven for drying;

[0212] 5. The dried corn cob particles are respectively placed in naphthalene acetic acid solutions with concentrations of 0.3, 0.6, 0.9, 1.2, 1.5 mg / L (N1-N5) and gibberellins with concentrations of 50, 100, 150, 200, 250 mg / L (G1-G5) for soaking, and are taken out after 24 hours of soaking and drained of water. CK is soaked in water;

[0213] 6. Select uniform-sized and full-grain Mongolian ice grass seeds, and spray them with 0.05% sodium hypochlorite solution for disinfection. After 2-3 seconds of contact with the sodium hypochlorite solution, rinse the seeds thoroughly with distilled water at a temperature of 5-8°C;

[0214] 7. Mix the disinfected Mongolian ice grass seeds, the corn cob particles treated in step 5, and carboxymethyl cellulose by stirring, with a mixing mass ratio of 1:100:0.2, and control the stirring temperature at 8°C;

[0215] 8. Press the mixture obtained in step 7 into blocks to form Mongolian ice grass seed packets in the shape of cubes with lengths, widths, and heights of about 10-15 cm. Each seed packet contains about 50 Mongolian ice grass seeds.

[0216] 9. Place the prepared seed packets in a greenhouse with an environmental temperature of 25°C, and plant them in flowerpots for cultivation. Plant 1 seed packet in each flowerpot (diameter 50 cm), simulate the annual precipitation environment in arid areas, and set up gradient precipitation of P1 (150%), P2 (125%), P3 (100%), P4 (75%), and P5 (50%) times the environmental precipitation (about 185 mm in the central arid region of Ningxia) for seed water replenishment. Observe the seed germination rate after one growth cycle;

[0217] Results are shown in the following table: Figure 8 The results show that:

[0218] (1) The addition of gibberellin inhibits the germination of Mongolian ice grass seed packets;

[0219] (2) When the environment is in normal precipitation conditions (P3), the seed germination rate of the seed package with naphthalene acetic acid added at N1-N3 concentration can be increased by about 14.7%-33.4%;

[0220] (3) When the environment is in drought conditions (P4), the seed germination rate of the seed package with naphthalene acetic acid added at N1-N5 concentration can be increased by about 1.3%-28%, and the seed germination rate of the seed package with naphthalene acetic acid added at N4 concentration is the highest, about 28%;

[0221] (4) When the environment is in extreme drought conditions (P5), the seed germination rate of the seed package with naphthalene acetic acid added at N2 concentration can be increased from 0 to 17.3%.

[0222] The above only describes the preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method of promoting seed germination of Agropyron cristatum under extremely dry conditions, characterized in that, The Elymus nutans seed is embedded in the surface layer of soil, and if the environmental osmotic potential measured in the field is in the range of -1.5 MPa < environmental osmotic potential < -1 MPa, the Elymus nutans seed package with gibberellin is selected; Or if the environmental osmotic potential measured in the field is in the range of -1.5 MPa < environmental osmotic potential < -1.2 MPa, the Elymus nutans seed package with naphthalene acetic acid is selected; The preparation method of the Elymus nutans seed package comprises the following steps: mixing the Elymus nutans seeds to be treated with porous materials with plant hormones or with auxin analogues, and coating agents according to a mass ratio of 1-2:100:0.2 to obtain the Elymus nutans seed package; the plant hormones are selected from gibberellin, and the auxin analogues are selected from naphthalene acetic acid; the porous materials are corn cobs; the coating agents are selected from at least one of water-retaining agents, antifreeze agents, penetrants, film-forming agents, adhesives, and fillers; the water-retaining agent is carboxymethyl cellulose; The porous materials with plant hormones are prepared by the following method: soaking the porous materials in a solution of naphthalene acetic acid with a concentration of 0.3-0.9 mg / L; The porous materials with auxin analogues are prepared by the following method: soaking the porous materials in a solution of gibberellin with a concentration of 50-250 mg / L.

2. The method of facilitating germination of Agropyron seed under conditions of extreme drought according to claim 1, wherein, If the environmental osmotic potential measured in the field is in the range of -1.2 MPa < environmental osmotic potential < -1 MPa, the Elymus nutans seed package with gibberellin is selected.

3. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 1, characterized in that, The porous materials are soaked in a solution of naphthalene acetic acid with a concentration of 0.3-0.9 mg / L for 6-24 hours, and then taken out and drained.

4. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 3, characterized in that, The porous materials are soaked in a solution of gibberellin with a concentration of 50-250 mg / L for 6-24 hours, and then taken out and drained.

5. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 4, characterized in that, The soaking time is 12 hours.

6. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 1, wherein, After the Elymus nutans seeds to be treated are mixed with the porous materials with plant hormones or with auxin analogues and coating agents, stirring is further included; the stirring temperature is less than 10°C.

7. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 6, characterized in that, The stirring temperature is 5-8°C.

8. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 1, wherein, Both the mixing of the Elymus nutans seeds to be treated with the porous materials with plant hormones or with auxin analogues and the coating agents and the stirring process are completely in the dark.

9. The method of enhancing seed germination of Agropyron cristatum under extreme drought conditions according to claim 8, characterized in that, The antifreeze agent is glycerol; the penetrant is a water-soluble thione; the film-forming agent is chitosan; the adhesive is selected from at least one of phenylpropyl emulsion and carboxymethyl cellulose; and the filler comprises at least one of bentonite and talcum powder.

10. A method of promoting seed germination of Agropyron mongolicum under drought conditions, characterized by, The Agropyron mongolicum seed is embedded in the surface layer of soil, and if the environmental osmotic potential measured in the field is in the range of -0.5 MPa ≤ environmental osmotic potential < 0 MPa, the Agropyron mongolicum seed package with naphthalene acetic acid is selected; the porous materials with naphthalene acetic acid are prepared by the following method: soaking the porous materials in a solution of naphthalene acetic acid with a concentration of 0.3 mg / L < naphthalene acetic acid concentration ≤ 0.6 mg / L; and then the porous materials with naphthalene acetic acid are used to prepare the Agropyron mongolicum seed package with naphthalene acetic acid; If the environmental osmotic potential measured in the field is in the range of -1.1 MPa < environmental osmotic potential < -0.5 MPa, the Agropyron mongolicum seed package with naphthalene acetic acid is selected; the porous materials with naphthalene acetic acid are prepared by the following method: soaking the porous materials in a solution of naphthalene acetic acid with a concentration of 0.6 mg / L < naphthalene acetic acid concentration ≤ 1.2 mg / L; Then the porous material with naphthalene acetic acid is used to prepare the seed package of Mongolian ice grass with naphthalene acetic acid; The preparation method of the seed package of Mongolian ice grass comprises the following steps: mixing the seed of Mongolian ice grass to be treated with the porous material with auxin analog, and coating agent according to the mass ratio of 1-2:100:0.2 to obtain the seed package of Mongolian ice grass; the auxin analog is selected from naphthalene acetic acid; the porous material is corn cob; the coating agent is selected from at least one of water-retaining agent, antifreezing agent, penetrating agent, film-forming agent, adhesive and filling agent; the water-retaining agent is carboxymethyl cellulose.

11. The method for promoting seed germination of Agropyron mongolicum under drought conditions according to claim 10, characterized in that, The porous material with auxin analog is prepared by the following method: soaking the porous material in the solution of 0.3-1.2 mg / L naphthalene acetic acid.

12. The method for promoting seed germination of Agropyron mongolicum under drought conditions according to claim 11, characterized in that, Soaking for 6-24 h, and then taking out and draining.

13. The method for promoting seed germination of Agropyron mongolicum under drought conditions according to claim 12, characterized in that, Soaking for 12 h.

14. The method for promoting seed germination of Agropyron mongolicum under drought conditions according to claim 10, characterized in that, After mixing the seed of Mongolian ice grass to be treated with the porous material with auxin analog and coating agent, stirring is further included; the stirring temperature is less than 10℃.

15. The method for promoting seed germination of Agropyron mongolicum under drought conditions according to claim 14, characterized in that, The stirring temperature is 5-8℃.

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