A lateral flow immunoassay kit and method for detecting aflatoxin B1

By employing a biotin-streptavidin directional conjugation strategy and smartphone photography technology, a nanobody LFIA test strip was developed, which solved the problem of the nanobody activity being affected and achieved rapid, accurate, and stable on-site detection of aflatoxin B1, with broad application potential.

CN116840460BActive Publication Date: 2026-01-30INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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Patent Information

Application Number
CN202310516218.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-05-09
Publication Date
2026-01-30
Estimated Expiration
2043-05-09

AI Technical Summary

Technical Problem

In existing technologies, the activity of nanobody-based lateral flow immunoassay test strips is easily affected during the connection process between the nanobody and the nanomaterial when detecting mycotoxins, resulting in unsatisfactory performance. Furthermore, traditional methods are complex and time-consuming, making it difficult to achieve rapid and accurate on-site detection.

Method used

A biotin/streptavidin nanobody-biotin/streptavidin@quantum dot probe was prepared using a biotin-streptavidin directional conjugation strategy. Combined with smartphone photography technology, a lateral flow immunoassay kit was developed for the detection of aflatoxin B1.

Benefits of technology

It achieves significant retention of nanobody activity, with a detection limit as low as 0.106 ng·mL⁻¹ and an IC50 of 0.86 ng·mL⁻¹. It has the capability of rapid, accurate, stable, and instrument-free on-site analysis, and exhibits high stability and tolerance in complex environments.

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Abstract

This invention discloses a lateral immunoassay kit and method for detecting aflatoxin B1, belonging to the field of lateral immunoassay technology. The kit includes a nanobody-biotin / streptavidin@quantum dot probe and a lateral immunoassay test strip. The lateral immunoassay test strip has a T-line and a C-line on its nitrocellulose membrane. The T-line is coated with AFB1-BSA antigen, and the C-line is coated with biotin-labeled bovine serum albumin. The directional coupling strategy of this invention overcomes the defect of reduced nanobody activity caused by random modification, greatly preserving the activity of the nanobody. Utilizing smartphone photography for grayscale reading, it features rapid, accurate, stable, and instrument-free on-site analysis, possessing broad application potential and significant market prospects.
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