An array-type multi-field lensless microscopy imaging system

Abstract

The present invention relates to the field of biological microscopic imaging technology, and specifically provides an array-type multi-field lensless microscopic imaging system, which aims to solve the problems in the prior art of poor precision and poor stability of multi-field imaging technology, resulting in unsatisfactory imaging effects, and the time-consuming displacement of the displacement stage. The present invention includes a lighting system, including a plurality of LED lamps; a plurality of image sensors arranged on a base, which are arranged below the lighting system, and each image sensor corresponds to a plurality of LED lamps for lighting from different angles. The present invention integrates a plurality of image sensors on a base, so that when observing different samples, there is no need to move the lighting system or the image sensor, thereby avoiding the problems of low precision and instability caused by the movement of the displacement stage, as well as the time-consuming displacement, thereby achieving better imaging effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological microscopic imaging, and in particular relates to an array-type multi-field lensless microscopic imaging system. Background Art

[0002] Achieving multi-field imaging is of great significance in microscopy, as it can improve imaging efficiency, enable simultaneous imaging of multiple control groups, and enable large sample imaging through stitching. In existing technologies, multi-field imaging is generally achieved through automatic control of a high-precision microscope XY stage and mechanical scanning. However, when imaging different samples, the stage needs to be controlled to move between them, which can lead to the stage not being able to accurately align with the sample, resulting in low imaging accuracy. Furthermore, the stage can shake when moving, causing unstable imaging. Furthermore, the stage movement process is relatively time-consuming. To minimize the negative impact of these characteristics, it is necessary to continuously pursue high-precision, high-synchronization, and high-response XY stages and control methods, but this increases the cost of the control system and makes implementation more difficult. Summary of the Invention

[0003] The present invention provides an array-type multi-field lensless microscopic imaging system, which aims to solve the problems in the prior art of multi-field imaging technology, such as poor precision and stability leading to unsatisfactory imaging effects, and the time-consuming displacement of the translation stage.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] The present invention provides an array-type multi-field lensless microscopic imaging system, comprising:

[0006] A lighting system, including several LED lights;

[0007] A plurality of image sensors are arranged below the lighting system, and each image sensor corresponds to a plurality of LED lights that illuminate from different angles;

[0008] A base, wherein the plurality of image sensors are arranged in a central area of ​​the base;

[0009] A microcontrol system is connected to the lighting system and the image sensor, and the microcontrol system controls the switch of the LED light and controls the image sensor to acquire an image.

[0010] A further solution is that a plurality of image sensors are arranged in an array on the base.

[0011] Based on the above solution, the array arrangement of several image sensors can improve the integration level of the array-type multi-field lensless microscopic imaging system of the present invention.

[0012] A further solution is that each of the image sensors corresponds to an independent group of LED lights that illuminate from different angles; or, several of the image sensors share a group of LED lights that illuminate from different angles.

[0013] A further solution: the lighting system includes: a plurality of groups of double-arc light strips; the double-arc light strips correspond to the image sensors one by one and form a plurality of field of view groups; the plurality of field of view groups are arranged on the base, and the plurality of groups of double-arc light strips are gathered together and arranged crosswise;

[0014] The double arc light strip comprises two arc light strips whose planes are perpendicular to each other, and each arc light strip is provided with a plurality of LED lights at equal intervals;

[0015] The image sensor is arranged directly below the intersection of the two arc light strips, and the distance from each LED lamp to the detection position of the image sensor is equal.

[0016] Based on this solution, each image sensor corresponds to one dual-arc light strip, allowing the sample at each image sensor's detection position to be fully illuminated by the LED lights on the dual-arc light strip, thereby allowing the image sensor to obtain a comprehensive image of the sample. Furthermore, by combining multiple sets of dual-arc light strips and crisscrossing them, the array-type multi-field lensless microscopy system can be made smaller without compromising imaging quality.

[0017] A further solution: there are four field of view groups; the four image sensors are respectively arranged at the four corners of the square area on the base.

[0018] A further solution is that a light strip mounting portion is provided on the base, and the four groups of double-arc light strips are all arranged on the base through the light strip mounting portion, and the four groups of double-arc light strips all pass over the area where the four image sensors are located from above.

[0019] Based on the above solution, the double-arc light strip passes over the area where the four image sensors are located from above, which can prevent interference and obstruction from the double-arc light strips in other viewing angle groups.

[0020] A further solution: one of the two arc light strips in each field of view group is arranged horizontally and the other is arranged vertically;

[0021] The horizontal arc light strips in the first field of view group are respectively crossed and located above the longitudinal arc light strips in the second field of view group, above the longitudinal arc light strips in the third field of view group, and below the longitudinal arc light strips in the fourth field of view group; the longitudinal arc light strips in the first field of view group are respectively crossed and located above the horizontal arc light strips in the fourth field of view group, below the horizontal arc light strips in the second field of view group, and below the horizontal arc light strips in the third field of view group;

[0022] The horizontal arc light strips in the second field of view group are respectively crossed and located above the longitudinal arc light strip in the first field of view group, below the longitudinal arc light strip in the third field of view group, and below the longitudinal arc light strip in the fourth field of view group; the longitudinal arc light strips in the second field of view group are respectively crossed and located below the horizontal arc light strip in the first field of view group, above the horizontal arc light strip in the fourth field of view group, and above the horizontal arc light strip in the third field of view group;

[0023] The horizontal arc light strips in the third field of view group are respectively crossed and located below the longitudinal arc light strip in the second field of view group, above the longitudinal arc light strip in the first field of view group, and above the longitudinal arc light strip in the fourth field of view group; the longitudinal arc light strips in the third field of view group are respectively crossed and located above the horizontal arc light strip in the second field of view group, below the horizontal arc light strip in the fourth field of view group, and below the horizontal arc light strip in the first field of view group;

[0024] The horizontal arc light strips in the fourth field of view group are respectively crossed and located above the longitudinal arc light strip in the third field of view group, below the longitudinal arc light strip in the first field of view group, and below the longitudinal arc light strip in the second field of view group; the longitudinal arc light strips in the fourth field of view group are respectively crossed and located above the horizontal arc light strip in the first field of view group, above the horizontal arc light strip in the second field of view group, and below the horizontal arc light strip in the third field of view group.

[0025] Based on the above solution: the cross setting of multiple groups of double arc light strips can not only reduce the occupied space of the double arc light strips on the basis of multiple fields of view and achieve the highest integration; but also can achieve the least obstruction of the LED lights by multiple groups of double arc light strips, thereby achieving better imaging effect.

[0026] A further embodiment of the present invention is a method for obtaining a sample image comprising the following steps:

[0027] S100, the microcontroller controls each LED light on each group of the double-arc light strips to light up in sequence, and controls the corresponding image sensor to take pictures of the sample to obtain sample images at different angles; the image sensor temporarily stores the obtained sample images in the microcontroller;

[0028] S200 , repeating step S100 for each of the remaining field of view groups in sequence, so that each field of view group obtains a sample image corresponding to a different angle of the sample.

[0029] A further solution: each detection position of the image sensor is provided with a sample slot.

[0030] Based on the above solution, the sample tank facilitates the placement of liquid samples on the detection position of the image sensor.

[0031] A further solution: the microcontrol system is connected to a computer.

[0032] The beneficial effects of the present invention are:

[0033] 1. The present invention integrates several image sensors on a base, and several image sensors are arranged below the illumination system to form a static and multi-field microscope system. When observing different samples, there is no need to move the illumination system or the image sensor, thus avoiding the low precision and instability caused by the movement of the translation stage, as well as the time-consuming displacement problems, thereby achieving better imaging effects.

[0034] 2. In the present invention, several image sensors are integrated into one base, which can make the array-type multi-field lensless microscopic imaging system smaller in size without affecting the imaging effect.

[0035] 3. The present invention does not require high-precision scanning devices or control means, and has low cost and low implementation difficulty. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0037] Figure 1 It is a structural schematic diagram of an array-type multi-field lensless microscopic imaging system of the present invention;

[0038] Figure 2 It is a schematic structural diagram of a visual field group in the present invention;

[0039] Figure 3 It is a structural schematic diagram of the base in the present invention.

[0040] Description of the numbers in the figure:

[0041] 1-Dual arc light strip; 2-LED light; 3-Image sensor; 4-Sample slot; 5-Base; 6-Light strip mounting portion; 7-Image sensor mounting portion. DETAILED DESCRIPTION

[0042] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0043] like Figure 1-3 As shown, this embodiment provides an array-type multi-field lensless microscopic imaging system, comprising:

[0044] A lighting system including a plurality of LED lights 2;

[0045] A plurality of image sensors 3 are arranged below the lighting system, and each image sensor 3 corresponds to a plurality of LED lights 2 that illuminate from different angles;

[0046] A base 5, wherein the plurality of image sensors 3 are arranged in a central area of ​​the base 5;

[0047] The micro control system is connected to the lighting system and the image sensor 3 , and the micro control system controls the switch of the LED light 2 and controls the image sensor 3 to acquire an image.

[0048] The plurality of image sensors 3 are arranged in an array on the base 5. The array includes a circular array and / or a rectangular array.

[0049] The image sensor 3 may be a CMOS (Complementary Metal-Oxide-Semiconductor) image sensor, a CCD (Charge Coupled Device) image sensor, or the like.

[0050] The microcontrol system can adopt Raspberry Pi, single-chip microcomputer, FPGA (Field Programmable Gate Array) or Jetson Nano (a powerful embedded system) and the like.

[0051] For the lighting system, one of the solutions is:

[0052] The lighting system includes an annular light strip disposed transversely above the plurality of image sensors 3. The annular light strip is provided with equally spaced LED lights 2, each spaced at equal polarization angles. The plurality of image sensors 3 share the LED lights 2 on the annular light strip, each illuminating from different angles.

[0053] Among them, a plurality of the image sensors 3 and the annular light strip are connected to a micro control system, and the micro control system controls the switch of the LED light 2 and controls the image sensor 3 to acquire an image.

[0054] Another option is:

[0055] The lighting system includes: a plurality of sets of double-arc light strips 1; each of the image sensors 3 corresponds to an independent set of LED lights 2 that illuminate from different angles; that is, the double-arc light strips 1 correspond to the image sensors 3 one-to-one, forming a plurality of field of view groups; the plurality of field of view groups are arranged on the base 5, and the plurality of sets of double-arc light strips 1 are converged and arranged crosswise;

[0056] The double arc light strip 1 comprises two arc light strips whose planes are perpendicular to each other, and each arc light strip is provided with a plurality of LED lights 2 at equal intervals;

[0057] The image sensor 3 is arranged directly below the intersection of the two arc light strips, and the distance from each LED lamp 2 to the detection position of the image sensor 3 is equal.

[0058] like Figure 2 As shown, a specific example of a single field of view group in the above solution is:

[0059] The two arc light strips on the double arc light strip 1 are arranged in a horizontal direction and a vertical direction, respectively.

[0060] The number of the LED lamps 2 arranged on the double arc light strip 1 is as large as possible while adjacent LED lamps 2 do not overlap with each other; the number of the LED lamps 2 on the arc light strip can be 39, 41 or 43, etc.

[0061] The polarization angles of the intervals between each of the LED lights 2 on the double-arc light strip 1 are equal; for example, when the number of the LED lights 2 on the double-arc light strip 1 is 41, the polarization angles of the intervals between each of the LED lights 2 are 6 degrees.

[0062] The distance between each LED lamp 2 on the double-arc light strip 1 and the sample can be 9 cm, 10 cm, or 11 cm.

[0063] In addition, it should be noted that the number of the LED lights 2 on the double-arc light strip 1, the polarization angle between each LED light 2, and the distance from the LED light 2 to the detection position of the image sensor 3 can be adjusted according to actual conditions.

[0064] The array-type multi-field lensless microscopic imaging system constructed by forming a plurality of field-of-view groups by one-to-one correspondence between the double-arc light strips 1 and the image sensors 3 is specifically as follows based on any of the above solutions:

[0065] The image sensors 3 are arranged in a rectangular array on the base 5. The lateral distance between adjacent image sensors 3 is equal, and the longitudinal distance between adjacent image sensors 3 is equal, and this distance can be adjusted based on actual conditions. For example, the image sensors 3 can be arranged with a lateral distance of 6 cm and a longitudinal distance of 6 cm.

[0066] The base 5 is provided with a light strip mounting portion 6 for mounting the double arc light strip 1 and an image sensor mounting portion 7 for mounting the image sensor 3 .

[0067] Some preferred cases are: Figure 1 and Figure 3 As shown, there are four field of view groups; the four image sensors 3 are arranged at the four corners of the square area on the base 5 in a manner of being 6 cm apart in the horizontal direction and 6 cm apart in the vertical direction.

[0068] The four groups of double-arc light strips 1 are all arranged on the base 5 through the light strip mounting portion 6 , and the four groups of double-arc light strips 1 pass over the areas where the four image sensors 3 are located from above.

[0069] Specifically, such as Figure 3 As shown, the light strip mounting portion 6 is a plurality of steps arranged on the base 5 and close to the edge, wherein four steps form a group for mounting the four ends of a group of the double arc light strip 1 .

[0070] Four image sensor mounting parts 7 are provided in the central area of ​​the base 5 . The four image sensor mounting parts 7 are arranged with a horizontal distance of 6 cm and a vertical distance of 6 cm between them. The four image sensors 3 are respectively provided on the four image sensor mounting parts 7 .

[0071] like Figure 1 As shown, the crossing method of the double arc light strip 1 for the four viewing angle groups is as follows, wherein the four viewing angle groups are arranged in a clockwise order, namely, viewing angle group 1, viewing angle group 2, viewing angle group 3, and viewing angle group 4;

[0072] The horizontal arc light strips in the first field of view are respectively crossed and located above the longitudinal arc light strips in the second field of view group, above the longitudinal arc light strips in the third field of view group, and below the longitudinal arc light strips in the fourth field of view group; the longitudinal arc light strips in the first field of view are respectively crossed and located above the horizontal arc light strips in the fourth field of view group, below the horizontal arc light strips in the second field of view group, and below the horizontal arc light strips in the third field of view group;

[0073] The horizontal arc light strips in the second field of view group are respectively crossed and located above the longitudinal arc light strip in the first field of view, below the longitudinal arc light strip in the third field of view group, and below the longitudinal arc light strip in the fourth field of view group; the longitudinal arc light strips in the second field of view group are respectively crossed and located below the horizontal arc light strip in the first field of view, above the horizontal arc light strip in the fourth field of view group, and above the horizontal arc light strip in the third field of view group;

[0074] The horizontal arc light strips in the third field of view group are respectively crossed and located below the longitudinal arc light strip in the second field of view group, above the longitudinal arc light strip in the first field of view group, and above the longitudinal arc light strip in the fourth field of view group; the longitudinal arc light strips in the third field of view group are respectively crossed and located above the horizontal arc light strip in the second field of view group, below the horizontal arc light strip in the fourth field of view group, and below the horizontal arc light strip in the first field of view;

[0075] The horizontal arc light strips in the four groups of visual fields are respectively crossed and located above the longitudinal arc light strips in the three groups of visual fields, below the longitudinal arc light strips in the one group of visual fields, and below the longitudinal arc light strips in the two groups of visual fields; the longitudinal arc light strips in the four groups of visual fields are respectively crossed and located above the horizontal arc light strips in the one group of visual fields, above the horizontal arc light strips in the two groups of visual fields, and below the horizontal arc light strips in the three groups of visual fields.

[0076] Based on any of the above solutions, each field of view group is connected to a micro-control system, which controls the switching of the corresponding LED light 2 and controls the image sensor 3 to acquire images. Multiple micro-control systems are connected to the computer.

[0077] In some alternative solutions, the combination of the microcontrol system and the computer can be replaced by any chip with high-speed computing capabilities, such as a DSP (Digital Signal Processing) chip, an FPGA (Field Programmable Gate Array) or an ASIC (Application Specific Integrated Circuit). These chips can achieve both high-speed computing and control functions.

[0078] In this embodiment, the method for obtaining a sample image by using an array-type multi-field lensless microscopy imaging system includes the following steps:

[0079] S100, the microcontroller controls each LED light on each group of the double-arc light strips to light up in sequence, and controls the corresponding image sensor to take pictures of the sample to obtain sample images at different angles; the image sensor temporarily stores the obtained sample images in the microcontroller;

[0080] S200 , repeating step S100 for each of the remaining field of view groups in sequence, so that each field of view group obtains a sample image corresponding to a different angle of the sample.

[0081] Taking four viewing angle groups, and each group of the double arc light strip 1 being provided with 41 LED lights 2 as an example, the specific case of the above solution is as follows:

[0082] In the first step, the microcontrol system corresponding to the microscope group controls the first LED light 2 on the double arc light strip 1 to light up, and controls the image sensor 3 to take a picture of the sample to obtain a sample image at a first angle; the image sensor 3 temporarily stores the sample image in the corresponding microcontrol system; and the microcontrol system controls the first LED light 2 to turn off;

[0083] In the second step, the microcontrol system corresponding to the microscope group controls the second LED light 2 on the double arc light strip 1 to light up, and controls the image sensor 3 to take a picture of the sample to obtain a sample image at a second angle; the image sensor 3 temporarily stores the sample image in the corresponding microcontrol system; the microcontrol system controls the second LED light 2 to turn off; and so on, until the microcontrol system controls the 41st LED light 2 to turn off; the microcontrol system corresponding to the microscope group stores 41 sample images of the samples in the microscope group;

[0084] In the third step, the microcontrol system corresponding to the second microscope group controls the first LED light 2 on the double arc light strip 1 to light up, and controls the image sensor 3 to take a picture of the sample to obtain a sample image at a first angle; the image sensor 3 temporarily stores the sample image in the corresponding microcontrol system; the microcontrol system controls the first LED light 2 to turn off; and so on, until the microcontrol system controls the 41st LED light 2 to turn off; the microcontrol system corresponding to the second microscope group stores 41 sample images of the samples in the second microscope group;

[0085] In the fourth step, the microcontrol system corresponding to the three microscope groups controls the first LED light 2 on the double arc light strip 1 to light up, and controls the image sensor 3 to take a picture of the sample to obtain a sample image at a first angle; the image sensor 3 temporarily stores the sample image in the corresponding microcontrol system; the microcontrol system controls the first LED light 2 to turn off; and so on, until the microcontrol system controls the 41st LED light 2 to turn off; the microcontrol system corresponding to the three microscope groups stores 41 sample images of the samples in the three microscope groups;

[0086] Step 5: The microcontrol system corresponding to the four microscope groups controls the first LED light 2 on the double arc light strip 1 to light up, and controls the image sensor 3 to take a picture of the sample to obtain a sample image at a first angle; the image sensor 3 temporarily stores the sample image in the corresponding microcontrol system; the microcontrol system controls the first LED light 2 to turn off; and so on, until the microcontrol system controls the 41st LED light 2 to turn off; the microcontrol system corresponding to the four microscope groups stores 41 sample images of the samples in the four microscope groups;

[0087] On the basis of the above scheme, the corresponding microcontrol systems in the microscope group 1, the microscope group 2, the microscope group 3 and the microscope group 4 compress, name and transmit 41 sample images to the computer, and the computer performs three-dimensional reconstruction of the samples in the microscope group 1, the samples in the microscope group 2, the samples in the microscope group 3 and the samples in the microscope group 4 respectively through the received sample images; the specific three-dimensional reconstruction algorithm comes from Zheng W, Wang J, Zhou Y, et al. Lensless holographic microscope with a time and memory-saving algorithm for large-volume imaging of organoids[J]. Optics Letters, 2023, 48(3): 771-774. (published in the journal Optics Letters, the article is titled: A lensless holographic microscope for large-volume organoid imaging through a time-saving and memory-saving algorithm, and the authors are Zheng Weiqiang (co-first author), Wang Juan (co-first author), Zhou Yunhong, Zeng Qiang, Zhang Cheng, Liu Li, Yu Hui and Yang Yuting (corresponding authors)).

[0088] Among them, the process of the microcontrol system corresponding to the first microscope group transmitting the sample image to the computer and the process of the computer performing three-dimensional reconstruction of the sample is carried out in parallel with the process of the microcontrol system corresponding to the second microscope group controlling the switching of the LED light 2. Similarly, the process of the microcontrol system corresponding to the second microscope group transmitting the sample image to the computer and the process of the computer performing three-dimensional reconstruction of the sample is carried out in parallel with the process of the microcontrol system corresponding to the third microscope group controlling the switching of the LED light 2. The process of the microcontrol system corresponding to the third microscope group transmitting the sample image to the computer and the process of the computer performing three-dimensional reconstruction of the sample is carried out in parallel with the process of the microcontrol system corresponding to the fourth microscope group controlling the switching of the LED light 2.

[0089] Specific examples for the above scheme are:

[0090] The detection position of the image sensor 3 in each field of view group is provided with a sample slot 4 for placing liquid samples. The bottom thickness of the sample slot 4 is ≤ 3 mm. It should be noted that the sample slot 4 does not necessarily have to be provided on the image sensor 3. When the sample is solid, the sample can be placed directly on the detection position of the image sensor 3 without the sample slot 4.

[0091] As for the sample tank 4, any device with a thin and transparent bottom, such as a glass slide, a microfluidic chip, a culture dish, etc., can be selected.

[0092] The present invention is not limited to the above-mentioned optional implementation modes. Anyone can derive other forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, any technical solution that falls within the scope defined by the claims of the present invention falls within the scope of protection of the present invention.

Claims

1. An array-type multi-field lensless microscopic imaging system, characterized in that: include: A lighting system, including several LED lights; A plurality of image sensors are arranged below the lighting system, and each image sensor corresponds to a plurality of LED lights that illuminate from different angles; A base, wherein the plurality of image sensors are arranged in a central area of ​​the base; A microcontrol system is connected to the lighting system and the image sensor, and the microcontrol system controls the switching of the LED light and controls the image sensor to acquire an image; The lighting system includes: a plurality of groups of double-arc light strips; the double-arc light strips correspond to the image sensors one by one and form a plurality of field of view groups; the plurality of field of view groups are arranged on the base, and the plurality of groups of double-arc light strips are gathered together and arranged crosswise; The double arc light strip comprises two arc light strips whose planes are perpendicular to each other, and each arc light strip is provided with a plurality of LED lights at equal intervals; The image sensor is arranged directly below the intersection of the two arc light strips, and the distance from each LED lamp to the detection position of the image sensor is equal.

2. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: A plurality of image sensors are arranged on the base in an array.

3. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: Each of the image sensors corresponds to an independent group of LED lights that illuminate from different angles; or, several of the image sensors share a group of LED lights that illuminate from different angles.

4. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: There are four field of view groups; the four image sensors are respectively arranged at the four corners of the square area on the base.

5. The array-type multi-field lensless microscopic imaging system according to claim 4, characterized in that: The base is provided with a light strip mounting portion, and the four groups of double-arc light strips are all arranged on the base through the light strip mounting portion, and the four groups of double-arc light strips all pass over the areas where the four image sensors are located from above.

6. The array-type multi-field lensless microscopic imaging system according to claim 5, characterized in that: The two arc light strips in each field of view group are arranged in a horizontal direction and a vertical direction respectively; The horizontal arc light strips in the first field of view group are respectively crossed and located above the longitudinal arc light strips in the second field of view group, above the longitudinal arc light strips in the third field of view group, and below the longitudinal arc light strips in the fourth field of view group; the longitudinal arc light strips in the first field of view group are respectively crossed and located above the horizontal arc light strips in the fourth field of view group, below the horizontal arc light strips in the second field of view group, and below the horizontal arc light strips in the third field of view group; The horizontal arc light strips in the second field of view group are respectively crossed and located above the longitudinal arc light strip in the first field of view group, below the longitudinal arc light strip in the third field of view group, and below the longitudinal arc light strip in the fourth field of view group; The longitudinal arc light strips in the second field of view group are respectively crossed and located below the transverse arc light strips in the first field of view group, above the transverse arc light strips in the fourth field of view group, and above the transverse arc light strips in the third field of view group; The horizontal arc light strips in the third field of view group are respectively crossed and located below the longitudinal arc light strip in the second field of view group, above the longitudinal arc light strip in the first field of view group, and above the longitudinal arc light strip in the fourth field of view group; The longitudinal arc light strips in the third field of view group are respectively crossed and located above the transverse arc light strips in the second field of view group, below the transverse arc light strips in the fourth field of view group, and below the transverse arc light strips in the first field of view group; The horizontal arc light strips in the fourth field of view group are respectively crossed and located above the longitudinal arc light strip in the third field of view group, below the longitudinal arc light strip in the first field of view group, and below the longitudinal arc light strip in the second field of view group; the longitudinal arc light strips in the fourth field of view group are respectively crossed and located above the horizontal arc light strip in the first field of view group, above the horizontal arc light strip in the second field of view group, and below the horizontal arc light strip in the third field of view group.

7. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: The method for obtaining a sample image comprises the following steps: S100, the microcontrol system sequentially controls each LED light on each group of the double-arc light strips to light up, and controls the corresponding image sensor to take pictures of the sample to obtain sample images at different angles; the image sensor temporarily stores the obtained sample images in the microcontrol system; S200 , repeating step S100 for each of the remaining field of view groups in sequence, so that each field of view group obtains a sample image corresponding to a different angle of the sample.

8. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: Each detection position of the image sensor is provided with a sample slot.

9. The array-type multi-field lensless microscopic imaging system according to claim 1, characterized in that: The microcontrol system is connected to a computer.

Images