Microfluidic joint detection card shell and device

Abstract

The application discloses a microfluidic joint detection card shell and device, and relates to the technical field of reagent detection. The microfluidic joint detection card shell comprises a bottom plate and a cover plate, wherein the cover plate is connected with the bottom plate; the bottom plate has a stepped structure, a liquid storage groove, a flow guide channel and a sample loading area; the stepped structure is distributed in an arc line shape; a capillary layer is formed between the stepped structure and the cover plate; the liquid storage groove and the flow guide channel are located on both sides of the stepped structure; one end of the flow guide channel is connected with the stepped structure; the sample loading area is in communication with the other end of the flow guide channel; the flow guide channel and the sample loading area both have a plurality of; the cover plate is provided with a first flow guide channel; the first flow guide channel is located above the flow guide channel; and the projection of the first flow guide channel along the direction perpendicular to the cover plate is at least partially located on the stepped structure. The joint detection card shell can reduce the reading error or result deviation caused by the detection time difference and sample difference.

Description

Technical Field

[0001] This application relates to the field of reagent testing technology, and in particular to a microfluidic multi-detection cassette and device. Background Technology

[0002] In related technologies, each test item in a multi-test card corresponds to a sample well. Each sample application can only complete one test item, or a single well cannot ensure uniform sample distribution and simultaneous testing of all test items, making the testing operation cumbersome. The inability to apply samples simultaneously and uneven sample distribution can easily lead to sample differences that affect test results and reduce accuracy.

[0003] In one type of multi-sample test card, a sample storage tank is fitted to the bottom of the sample well, and a flow guide tube is fitted to the sample storage tank. The end of the flow guide tube contacts the test strip. This design can meet the requirements for multiple tests simultaneously. However, the sample dispensing operation and differences in the length of the flow guide tube can easily lead to uneven sample distribution and differences in the sample loading time of the test strip, resulting in errors in the results of the multiple tests. Furthermore, the size of the flow guide tube limits the liquid flow time due to capillary action, making it difficult to implement in practical applications. In another type of multi-sample test card, the five TORCH IgM tests are integrated into one test card, with each test corresponding to a sample well and a result observation window. The five-test IgM chromatographic test strip cannot fully interpret the user's infection status, and the five sample wells in this design make the sample dispensing operation cumbersome, easily causing errors in sample loading time and leading to deviations in the judgment results of sample differences. Summary of the Invention

[0004] This application aims to address at least one of the technical problems existing in the prior art. To this end, this application proposes a microfluidic multi-test device that enables multiple test strips to start testing simultaneously, while reducing the error in test results caused by testing operation and interpretation.

[0005] This application also proposes a microfluidic joint inspection device having the aforementioned microfluidic joint inspection cassette.

[0006] One embodiment of this application includes a microfluidic testing cassette, comprising a base plate and a cover plate, the cover plate being connected to the base plate; the base plate having a stepped structure, a liquid storage tank, a flow channel, and a sample loading area, the stepped structure being arc-shaped, a capillary layer being formed between the stepped structure and the cover plate, the liquid storage tank and the flow channel being located on both sides of the stepped structure, one end of the flow channel being connected to the stepped structure, and the sample loading area being connected to the other end of the flow channel, with multiple flow channels and sample loading areas; the cover plate is provided with a first flow channel, the first flow channel being located above the flow channel, and at least part of the projection of the first flow channel along a direction perpendicular to the cover plate being located on the stepped structure.

[0007] Furthermore, the stepped structure includes a first step, the first step having a first end face, and the distance between the first end face and the bottom of the liquid storage tank is 0.3 to 1.0 mm.

[0008] Furthermore, the stepped structure also includes a second step, the second step having a second end face, the distance between the second end face and the first end face being 0.3 to 1.0 mm.

[0009] Furthermore, the second end face and the cover plate define the capillary layer, the height of which is 0.3-0.6 mm.

[0010] Furthermore, the length of the diversion channel is 3 to 6 mm.

[0011] Furthermore, the cover plate is also provided with a first protrusion, the first flow channel is at least partially connected to the first protrusion, and the projection of the first protrusion in the vertical direction is located within the sample loading area.

[0012] Furthermore, the first flow channel extends through the first protrusion.

[0013] Furthermore, along the direction perpendicular to the cover plate, the first protrusion extends 0.2 to 0.5 mm beyond the first flow channel.

[0014] Furthermore, the base plate is provided with a second flow channel, one end of which is connected to the liquid storage tank, and the other end of which extends to the end face of the stepped structure.

[0015] Furthermore, the cover plate has a sample dispensing hole, the position of which corresponds to the liquid storage tank. The cover plate is also provided with a second protrusion, which surrounds the sample dispensing hole and is arranged radially.

[0016] Furthermore, along the direction close to the sample dispensing hole, the height of the second protrusion gradually decreases.

[0017] Another embodiment of this application provides a microfluidic joint inspection device, which includes the microfluidic joint inspection cassette as described above.

[0018] The aforementioned microfluidic joint detection cassette and microfluidic joint detection device have at least the following beneficial effects: during detection, the sample loading part of the test strip is placed in the sample loading area, and the sample to be tested in the storage tank reaches the sample loading area after passing through the stepped structure, the first guide channel and the guide channel, and comes into contact with the sample loading part of the test strip located in the sample loading area to complete the sample loading. The stepped structure is located on one side of the liquid storage tank, and the side of the stepped structure closest to the liquid storage tank is arc-shaped. This ensures that the distance from the sample to be tested in the liquid storage tank to each first guide channel or guide channel is equal, which helps to keep the time for the sample to reach each test strip consistent. This facilitates the even distribution of the sample to be tested in the liquid storage tank to each sample loading area, and also allows each test strip to start testing simultaneously. The sample to be tested is mixed in the liquid storage tank before being uniformly distributed to each sample loading area, avoiding detection errors and result deviations caused by sample differences, differences among multiple tested samples, and differences in detection time that may occur when adding samples in stages. It also improves the user's ease of operation. A capillary layer is formed between the stepped structure and the cover plate. When the sample volume exceeds the volume of the liquid storage tank, the capillary layer acts as a capillary force to evenly distribute the liquid to the inlet of each guide channel. At the same time, the cover plate is provided with a first guide channel, and the liquid at the inlet is self-driven and guided into the sample loading area by the capillary action of the first guide channel. Under the action of the first guide channel and capillary layer, the sample to be tested in the storage tank can be uniformly and self-driven to the sample loading area, which helps to reduce the error caused by the sample loading operation. In addition, the first guide channel and the guide channel can guide the sample to be tested to flow to the sample loading area. The cross-sectional area of ​​the guide channel is larger, which can transport more samples to be tested compared to setting only the first guide channel, thus shortening the sample loading time.

[0019] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0020] The present application will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0021] Figure 1 This is a schematic diagram of the overall structure of the microfluidic joint inspection card holder according to one embodiment of this application;

[0022] Figure 2 This is a top view schematic diagram of a microfluidic joint inspection card holder according to one embodiment of this application;

[0023] Figure 3 for Figure 2 Schematic diagram of the cross section of AA;

[0024] Figure 4 for Figure 3 A magnified view of part B in the middle section;

[0025] Figure 5 This is a schematic diagram of the structure of the bottom plate in a microfluidic joint inspection card case according to one embodiment of this application;

[0026] Figure 6 This is a top view of the bottom plate of the microfluidic joint inspection card case according to one embodiment of this application;

[0027] Figure 7 for Figure 6 Cross-sectional view of CC;

[0028] Figure 8 for Figure 7 A magnified view of part D in the middle;

[0029] Figure 9 This is a schematic diagram of the structure of the back of the cover plate according to one embodiment of this application.

[0030] Figure label:

[0031] 100. Base plate; 110. Stepped structure; 111. First step; 1111. First end face; 112. Second step; 1121. Second end face; 120. Liquid storage tank; 130. Flow channel; 140. Sample loading area; 150. Third protrusion; 160. Second flow channel; 170. Placement area; 180. Connecting column;

[0032] 200, Cover plate; 210, Sample dispensing hole; 220, Observation window; 230, First flow channel; 240, First protrusion; 250, Second protrusion. Detailed Implementation

[0033] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0034] In the description of this application, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0035] In the description of this application, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0036] In the description of this application, unless otherwise expressly defined, terms such as "setup," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this application in conjunction with the specific content of the technical solution.

[0037] In the description of this application, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0038] One embodiment of this application discloses a microfluidic testing device, including a microfluidic testing cassette and test strips. The microfluidic testing cassette includes a base plate 100, which is provided with a liquid storage tank 120, a sample loading area 140, and multiple placement areas 170 for placing test strips. The test strips are placed in the placement areas 170, and the sample loading portion of the test strips is located in the sample loading area 140. During testing, the sample to be tested in the liquid storage tank 120 flows to the sample loading area 140 and comes into contact with the sample loading portion located in the sample loading area 140, thereby achieving sample loading and initiating testing.

[0039] It should be noted that the microfluidic combined detection device of this application embodiment can be applied to the detection of TORCH IgG, IgM ten items, TORCHIgM five items, and four infectious diseases.

[0040] Another embodiment of this application discloses a microfluidic joint inspection card holder, such as Figures 1 to 4As shown, the microfluidic joint inspection casing includes a base plate 100 and a cover plate 200, which are connected to the base plate 100. The base plate 100 has a stepped structure 110, a liquid storage tank 120, a flow channel 130, and a sample loading area 140. The stepped structure 110 is arc-shaped, and a capillary layer is formed between the stepped structure 110 and the cover plate 200. The liquid storage tank 120 and the flow channel 130 are located on both sides of the stepped structure 110. One end of the flow channel 130 is connected to the stepped structure 110, and the sample loading area 140 is connected to the other end of the flow channel 130. Multiple flow channels 130 and sample loading areas 140 are present. The cover plate 200 has a first flow channel 230 located above the flow channel 130, and at least part of the projection of the first flow channel 230 along a direction perpendicular to the cover plate 200 lies on the stepped structure 110. The first flow channel 230 is a capillary channel that drives the sample to be tested from one side of the stepped structure 110 to the side of the upper sample area 140. The capillary layer generates capillary force on the sample to be tested, which can drive the sample to be tested to move in a specific direction. The capillary layer is formed between the stepped structure 110 and the cover plate 200. The capillary force of the capillary layer on the sample to be tested is limited. Therefore, the setting of the first flow channel 230 can further drive the sample to be tested in the flow channel 130 towards the upper sample area 140.

[0041] In practical applications, the number of structures such as the diversion channel 130, the sample loading area 140, and the first diversion channel 230 can be adjusted to meet different joint testing requirements.

[0042] In this embodiment, the stepped structure 110 is distributed in an arc shape, making the liquid storage tank 120 appear as a fan shape. The lengths of each guide channel 130 and each first guide channel 230 are consistent. This ensures that the sample to be tested in the liquid storage tank 120 reaches each sample loading area 140 at the same time, facilitating the simultaneous detection of multiple test strips.

[0043] During testing, the sample loading part of the test strip is placed in the sample loading area 140. The sample to be tested in the liquid storage tank 120 flows through the stepped structure 110, the first guide channel 230 and the 230 guide channel 130 and then reaches the sample loading area 140, and comes into contact with the sample loading part of the test strip to complete the sample loading.

[0044] In the embodiments of this application, the stepped structure 110 is located on one side of the liquid storage tank 120, and the side of the stepped structure 110 near the liquid storage tank 120 is arc-shaped, so that the distance from the sample to be tested in the liquid storage tank 120 to each first guide channel 230 or guide channel 130 is equal. This is beneficial to ensure that the time for the sample to be tested in the liquid storage tank 120 to reach each test strip is consistent, and also beneficial to ensure that the sample to be tested in the liquid storage tank 120 is evenly distributed to each sample loading area 140, so that each test strip can start testing simultaneously; after the sample to be tested is mixed in the liquid storage tank 120... The samples are then uniformly distributed to each sample loading area 140 to avoid detection errors and result deviations caused by factors such as sample differences, differences between multiple test items, and differences in detection time that exist in multiple sample loading. At the same time, it also improves the user's convenient operation experience. A capillary layer is formed between the stepped structure 110 and the cover plate 200. When the sample volume exceeds the volume of the liquid storage tank 120, the capillary layer plays a capillary role to distribute the liquid evenly to the inlet of each guide channel 130. At the same time, the cover plate 200 is provided with a first guide channel 230. The liquid at the inlet is self-driven and guided into the sample loading area under the capillary action of the first guide channel. Under the action of the first guide channel 230 and the capillary layer, the sample to be tested in the storage tank 120 can be uniformly and self-driven to the sample loading area 140, which helps to reduce the error caused by the sample loading operation. In addition, the first guide channel 230 and the guide channel 130 can guide the sample to be tested to flow to the sample loading area 140. The cross-sectional area of ​​the guide channel 130 is large, which can transport more samples to be tested, thus shortening the sample loading time.

[0045] In some embodiments of this application, see Figure 2 and Figure 3 As shown, the cover plate 200 is provided with an observation window 220 to facilitate observation of the test results.

[0046] In some embodiments of this application, see Figure 5 As shown, the base plate 100 and the cover plate 200 enclose a detection cavity. The base plate 100 is provided with a test strip placement area 170 to restrict the movement of the test strip within the detection cavity. Further, the sample loading area 140 is provided with a third protrusion 150, which supports the test strip placed within the sample loading area 140. Further, the base plate 100 is also provided with a connecting post 180, which is used to connect and fix to the cover plate 200.

[0047] In some embodiments of this application, see Figure 4 and Figure 5As shown, the stepped structure 110 includes a first step 111, which has a first end face 1111. The distance between the first end face 1111 and the bottom of the liquid storage tank 120 is 0.3–1.0 mm. Specifically, the first end face 1111 is the end face of the first step 111 closest to the cover plate 200. When adding the sample to be tested into the liquid storage tank 120, the first step 111 acts as a buffer, reducing the possibility of liquid in the liquid storage tank 120 overflowing the stepped structure 110 and entering the capillary layer before being properly mixed. This ensures that the sample is mixed before loading, facilitating the simultaneous testing of multiple test strips. The distance between the first end face 1111 and the bottom of the liquid storage tank 120, i.e., the height of the first step 111, is set to 0.3–1.0 mm to ensure that the sample in the liquid storage tank 120 has sufficient volume to meet the requirements of simultaneous loading and testing of multiple test strips. In practical applications, the height of the first step 111 can be set to 0.4mm, 0.5mm, 0.6mm, 0.7mm, 0.8mm, etc., as needed.

[0048] For some embodiments of this application, please refer to [link / reference]. Figure 4 and Figure 5 As shown, the stepped structure 110 also includes a second step 112, which has a second end face 1121. The distance between the second end face 1121 and the first end face 1111 is 0.3–1.0 mm. Specifically, the second end face 1121 is the end face of the second step 112 closest to the end cap. The second step 112 is higher than the first end face 1111 by a certain height, increasing the volume of the liquid storage tank 120. It is worth understanding that by changing the height of the second step 112, the volume of the liquid storage tank 120 can be changed.

[0049] In some embodiments of this application, the second end face 1121 and the cover plate 200 define a capillary layer, the height of which is 0.3-0.6 mm. Specifically, the gap between the second end face 1121 and the cover plate 200 forms the aforementioned capillary layer, and the distance between the second end face 1121 and the cover plate 200 is the height of the capillary layer. Setting the height of the capillary layer to 0.3-0.6 mm enables the capillary layer to form a self-driven capillary action, driving the sample to be tested in the liquid storage tank 120 to flow from the liquid storage tank 120 to the sample area 140. In practical applications, the height of the capillary layer can be adaptively adjusted as needed, and the specific value can be set to 0.3 mm, 0.4 mm, 0.5 mm, etc., which are not limited here.

[0050] In some embodiments of this application, the length of the flow channel 130 is 3–6 mm. Specifically, the flow channel 130 is adapted to the first flow channel 230. At the start of the testing process, the sample to be tested first flows through the first flow channel 230 to the sample loading area 140. Then, the remaining sample to be tested, guided by the first flow channel 230, flows along the flow channel 130 to the sample loading area 140 and contacts the test strip located in the sample loading area 140 to complete the sample loading. Setting the length of the flow channel 130 to 3–6 mm facilitates the simultaneous start of testing for multiple items. It should be noted that when the length of the guide channel 130 is less than 3 mm, the sample to be tested may directly flow into the guide channel 130 and directly enter the sample loading area 140 along the guide channel 130. In this case, the sample to be tested may not pass through the first guide channel 230, making it impossible to control the time for the sample to flow from the storage tank 120 to the sample loading area 140, which may result in multiple test strips not being able to be tested simultaneously. When the length of the guide channel 130 is greater than 6 mm, the time required for the sample to be tested to reach the sample loading area 140 through the first guide channel 230 and the guide channel 130 is too long. As an example, in some embodiments, the time required for the sample to reach the sample loading area 140 through the first guide channel 230 and the guide channel 130 is greater than 1 second. The sample loading speed is slow, which may easily lead to sample loading deviation and is not conducive to shortening the detection time.

[0051] In some embodiments of this application, the cover plate 200 is further provided with a first protrusion 240, and the first flow channel 230 is at least partially connected to the first protrusion 240, with the projection of the first protrusion 240 in the vertical direction located within the sample loading area 140. Specifically, the first protrusion 240 contacts the sample loading portion of the test strip to guide the sample to be tested in the first flow channel 230 to the sample loading portion of the test strip, thereby ensuring timely sample loading.

[0052] In some embodiments of this application, see Figure 9 As shown, the first flow channel 230 is disposed through the first protrusion 240. This allows the first flow channel 230 to be connected to one side of the test strip, which helps to balance the air pressure at both ends of the first flow channel 230, so that the sample to be tested in the liquid storage tank 120 can flow up to the sample area 140 along the first flow channel 230.

[0053] For some embodiments of this application, please refer to [link / reference]. Figure 9As shown, along a direction perpendicular to the cover plate 200, the first protrusion 240 extends 0.2–0.5 mm beyond the bottom surface of the first flow channel 230, allowing the sample to be tested, flowing through the first flow channel 230 to the sample loading area 140, to immediately enter the sample loading section of the test strip along the first protrusion 240. The sample loading section of the test strip is typically made of glass fiber, a porous and highly absorbent material. If the first protrusion 240 is not present or its height is less than 0.2 mm, the liquid at the end of the first flow channel 230 may not contact the sample loading section, preventing timely sample loading and testing. When the height of the first protrusion 240 is greater than 0.5 mm, it will tightly press against the sample loading section of the test strip, making sample loading difficult and preventing the test from being completed.

[0054] In some embodiments of this application, the base plate 100 is provided with a second flow channel 160. One end of the second flow channel 160 is connected to the liquid storage tank 120, and the other end of the second flow channel 160 extends to the end face of the stepped structure 110. Specifically, the second flow channel 160 extends from the bottom of the liquid storage tank 120 toward the flow channel 130, and the flow channel 130 is located on the extended side of the second flow channel 160. The second flow channel 160 can guide the flow of the sample to be tested toward the stepped structure 110. The provision of the second flow channel 160 can accelerate the flow of the sample to be tested from the liquid storage tank 120 to the stepped structure 110, thereby shortening the detection time. It should be noted that the end of the second flow channel 160 away from the liquid storage tank 120 is at a certain distance from the flow channel 130 to reduce the direct flow of the sample to be tested from the stepped structure 110 into the flow channel 130.

[0055] Furthermore, the end of the second flow channel 160 away from the liquid storage tank 120 and the end of the first flow channel 230 near the liquid storage tank 120 overlap at least partially in the direction perpendicular to the cover plate 200, so that after flowing along the second flow channel 160 to the stepped structure 110, it can continue to flow along the first flow channel 230 to the sample area 140, thereby further improving the sample loading efficiency and shortening the detection time.

[0056] In one embodiment of this application, see [link to embodiment]. Figures 4 to 8 As shown, the end of the second flow channel 160 that connects to the liquid storage tank 120 is set below the bottom surface of the liquid storage tank 120 to facilitate the flow of the sample to be tested in the liquid storage tank 120 to the second flow channel 160.

[0057] In some embodiments of this application, see Figures 1 to 4As shown, the cover plate 200 has a sample application hole 210, which corresponds to the position of the liquid storage tank 120. The cover plate 200 is also provided with a second protrusion 250, which is arranged radially around the sample application hole 210. The second protrusion 250 can guide the air present in the guide channel 130 and the first guide channel 230 in the form of bubbles to one side of the sample application hole 210 when the sample to be tested flows from the liquid storage tank 120 to the sample area 140. This serves to remove the gas in the guide channel 130 and the first guide channel 230, so as to avoid the air in the guide channel 130 and the first guide channel 230 from obstructing the movement of the sample to be tested to the sample area 140.

[0058] In some embodiments of this application, see Figure 4 As shown, the height of the second protrusion 250 gradually decreases along the direction near the sample dispensing hole 210. This facilitates the movement of the aforementioned air bubble towards the side near the sample dispensing hole 210. It should be understood that the height of the second protrusion 250 refers to the distance between the second protrusion 250 and the end face of the cover plate 200 in the direction perpendicular to the cover plate 200.

[0059] The embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this application. Furthermore, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other.

Claims

1. A microfluidic co-detection card shell, characterized in that, The bottom plate and the cover plate are connected in cooperation; The bottom plate has a stepped structure, a liquid storage groove, a flow guide channel and a sample loading area, the stepped structure is distributed in an arc shape, so that the liquid storage groove is in a fan shape, a capillary layer is formed between the stepped structure and the cover plate, the liquid storage groove and the flow guide channel are located on both sides of the stepped structure, one end of the flow guide channel is connected with the stepped structure, the sample loading area is in communication with the other end of the flow guide channel, and the flow guide channel and the sample loading area each have a plurality of; The cover plate is provided with a first flow guide channel, the first flow guide channel is a capillary channel, the first flow guide channel is located above the flow guide channel, and a projection of the first flow guide channel in a direction perpendicular to the cover plate is at least partially located on the stepped structure; The bottom plate is provided with a second flow guide channel, one end of the second flow guide channel is in communication with the liquid storage groove, and the other end of the second flow guide channel extends to an end face of the stepped structure; The stepped structure includes a first step, and the first step has a first end face; The stepped structure further includes a second step, and the second step has a second end face; The second end face and the cover plate define the capillary layer.

2. The microfluidic multiplexed assay cartridge of claim 1, wherein, The distance between the first end face and the bottom of the liquid storage groove is 0.3-1.0 mm.

3. The microfluidic multiplexed assay cartridge of claim 2, wherein, The distance between the second end face and the first end face is 0.3-1.0 mm.

4. The microfluidic multiplexed assay cartridge of claim 3, wherein, The height of the capillary layer is 0.3-0.6 mm.

5. The microfluidic multiplexed assay cartridge of claim 1, wherein, The length of the flow guide channel is 3-6 mm.

6. The microfluidic multiplexed assay cartridge of claim 1, wherein, The cover plate is further provided with a first protrusion, the first flow guide channel is at least partially connected with the first protrusion, and a projection of the first protrusion in a vertical direction is located in the sample loading area.

7. The microfluidic multiplexed assay cartridge of claim 6, wherein, The first flow guide channel is arranged through the first protrusion.

8. The microfluidic multiplexed assay cartridge of claim 6 or 7, wherein, In a direction perpendicular to the cover plate, the first protrusion protrudes from the first flow guide channel by 0.2-0.5 mm.

9. The microfluidic multiplexed assay cartridge of claim 1, wherein, The cover plate has a sample loading hole, the position of the sample loading hole corresponds to the liquid storage groove, the cover plate is further provided with a second protrusion, the second protrusion surrounds the sample loading hole and is arranged in a radial manner.

10. The microfluidic multiplexed assay cartridge of claim 9, wherein, In a direction close to the sample loading hole, the height of the second protrusion gradually decreases.

11. A microfluidic multiplexed testing device, comprising: The microfluidic joint detection card shell comprises the microfluidic joint detection card shell according to any one of claims 1-10.

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