Metal chelate chromatography medium and method for its production

Abstract

The present application relates to the technical field of protein purification, and particularly relates to a metal chelation chromatography medium and a preparation method thereof, which has a structure as shown in formula I: wherein Q is agarose microspheres; L is a connecting arm, contains 2-8 carbon atoms, and further contains one or more ether groups and / or one or more hydroxyl groups. The metal chelation chromatography medium of the present application can form 2 five-membered rings and 2 six-membered rings with chelated ions such as Ni 2+ , Co 2+ , Cu 2+ , Zn 2+ , Fe 2+ , etc. to occupy 3 coordination orbitals of, for example, Ni 2+ , and the other 2 nitrogen atoms occupy 2 coordination orbitals. In terms of structure, the binding capacity with Ni 2+ is more firm, and the binding is less likely to fall off. The remaining 1 coordination orbital does not affect the binding with a histidine tag.

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