Use of an antiredeposition composition, fabric detergent and biosurfactant

Abstract

The application provides a use of an anti-redeposition composition, a fabric detergent and a biosurfactant. The application provides a new use of a biosurfactant in fabric washing, the biosurfactant or the anti-redeposition composition containing the biosurfactant is produced by bacteria, is a green source material, is harmless to human bodies, is friendly to the environment, is biodegradable, is harmless in long-term use, and meets the development direction of green detergent; the biosurfactant not only has an anti-redeposition function, but also can synergize with other surfactants in the fabric washing process, and improves the cleaning power of the detergent.

Description

Technical Field

[0001] This application belongs to the field of daily chemical technology, specifically relating to the use of an anti-redeposition composition, a fabric detergent, and a biosurfactant. Background Technology

[0002] Biosurfactants are amphiphilic substances produced by microorganisms during metabolism under specific culture conditions. They possess not only the common properties of surfactants, such as good emulsifying, dispersing, cleaning, solubilizing, wetting, foaming, and surface tension reduction, but also advantages over surfactants produced through chemical synthesis or petroleum refining. These advantages include non-toxicity or low toxicity, biodegradability, low irritation, and good compatibility with humans and the environment. Furthermore, biosurfactants exhibit diverse structures, making them suitable for specialized applications. Biosurfactants mainly include lipopeptides, glycolipids, and phospholipids.

[0003] Lipopeptide biosurfactants are metabolic products produced during microbial fermentation. They are amphoteric substances composed of hydrophilic cyclic oligopeptides and hydrophobic fatty acid chains linked by lactone bonds. They mainly originate from microorganisms such as Bacillus and Streptomyces. Their molecular structure is asymmetrical, exhibiting strong polarity and amphiphilicity (both water and oil affinity), significantly reducing surface and interfacial tension. This type of surfactant mainly includes surfactants such as iturins and fengycin. Surfactins consist of a 12-17 carbon β-hydroxy fatty acid linked to a 7-α-amino acid cyclic heptapeptide linked by lactone bonds. Fengycin consists of a 14-17 carbon β-hydroxy fatty acid linked to a 10-amino acid cyclic peptide chain; the cyclic peptide structure of fengycin is formed by the peptide chain itself, without the β-hydroxy fatty acid participating. Iturins consist of a β-amino fatty acid and a peptide chain linked by amide bonds. Most bacterial metabolic products, such as itursin, have a heptapeptide peptide chain that forms a macrocycle with β-amino fatty acids containing 14-17 carbon atoms.

[0004] Glycolipid biosurfactants are lipids containing sugars but not phosphates, composed of glycosyl groups linked to long-chain fatty acids or hydroxy fatty acids. Based on their chemical structure, glycolipids can be classified into glycol esters, glycolol esters, and glycosides, such as rhamnolipids, sophorolipids, and mannose-erythritol esters. The hydrophilic group of rhamnolipids generally consists of 1-2 rhamnose molecules, while the hydrophobic group consists of 1-2 saturated or unsaturated fatty acid molecules of different carbon chain lengths. Depending on the number of rhamnose molecules in the hydrophilic group, rhamnolipids are usually classified into monorhamnolipids and dirhamnolipids, their composition mainly depending on the fermentation strain and culture medium conditions. Sophorolipids consist of two parts: a hydrophilic sophorose group (two glucose molecules linked by a β-1,2 glycosidic bond) and a hydrophobic saturated or unsaturated long-chain ω- (or ω-1) hydroxy fatty acid. Sophorolipids are classified into lactone and acid types, usually a mixture of both structures. Mannose erythritol ester is a nonionic biosurfactant composed of hydrophilic groups of polar sugar groups and hydrophobic groups of nonpolar fatty acyl chains.

[0005] Phospholipids are a class of biosurfactants with phosphate groups as hydrophilic groups, including two main categories: glycerophospholipids and sphingomyelins.

[0006] The purpose of using fabric detergents is to remove various types of dirt from fabrics (e.g., water-soluble dirt, particulate dirt, grease, or a combination of these). During washing, various dirt particles detach from the fabric surface and form an unstable dispersion in the water, causing these particles to redeposit on the fabric surface. After repeated washing, fabrics become stiff, rough, dull, and grayish due to dirt buildup, affecting not only their performance but also their lifespan. The detergency of fabric detergents includes primary and secondary detergency. Primary detergency refers to the detergent removing dust, protein, grease, and other dirt from the fabric and dispersing them in the water. Secondary detergency prevents the dispersed dirt in the water from redepositing onto the fabric. However, the secondary detergency of detergents is relatively poor. To address this issue, anti-redeposition agents are typically added to the detergent. Anti-redeposition agents can function through various mechanisms. It is generally believed that anti-redeposition agents can suspend dirt in water, effectively preventing dirt from redepositing onto fabrics, thereby improving problems such as hardening, roughness, and dullness of fabrics after washing, and enhancing the washing performance of detergents.

[0007] Anti-redeposition agents play a crucial role in various forms of detergents, such as laundry liquid and laundry pods. my country is a major global producer of synthetic detergents, resulting in a large demand for anti-redeposition agents. As liquid detergents trend towards concentration and eco-friendliness, market requirements for anti-redeposition agents are also gradually shifting towards greener and more efficient formulations.

[0008] Currently used anti-redeposition agents are mostly polymers, such as sodium polyacrylate, carboxymethyl cellulose, and styrene-acrylic acid copolymers. Existing technologies add anti-redeposition agents to liquid detergents, but some reduce the detergent's transparency and stability; others act as thickeners, significantly increasing the detergent's viscosity, complicating the production process, and making them unsuitable for highly concentrated formulations; still others only achieve anti-redeposition effects on certain fabric materials. Each has its own advantages and disadvantages, and none have demonstrated sufficient effectiveness. Summary of the Invention

[0009] In view of the problems existing in the prior art, this application provides a green, environmentally friendly, biodegradable, safe and efficient anti-redeposition composition, fabric detergent and use of biosurfactant.

[0010] Specifically, this application relates to the following aspects: 1. An anti-redeposition composition comprising 0.0001wt%-99.9999wt% of a biosurfactant as an active ingredient for preventing dirt redeposition.

[0011] 2. The anti-redeposition composition according to claim 1, wherein the dirt is dirt washed off from the fabric.

[0012] 3. The anti-redeposition composition according to item 1 or 2, wherein the biosurfactant is the sole active ingredient for preventing dirt redeposition.

[0013] 4. The anti-redeposition composition according to any one of items 1-3, wherein the biosurfactant is selected from one or more of lipopeptides, glycolipids and phospholipids.

[0014] 5. The anti-redeposition composition according to claim 4, wherein the lipopeptide is selected from one or more of the group consisting of: surfactants, iturobrine, fenbufen, lichenin, babylin, permiracin, spore-forming fungicides, antifungal fungicides, phosphatidylin, fusarium fungicides, kurstakin, spore-forming fungicides, polymyxins, octapeptide, and polypeptide fungicides.

[0015] 6. The anti-redeposition composition according to item 4, wherein the glycolipid is selected from one or more of the group consisting of: rhamnolipid, sophorolipid, mannose erythritol lipolipid, trehalose lipolipid, and cellobiose lipolipid.

[0016] 7. The anti-redeposition composition according to item 4, wherein the phospholipid is selected from one or more of the group consisting of lecithin, cephalin, inositol phospholipid, serine phospholipid, and phosphorylethanolamine.

[0017] 8. The anti-redeposition composition according to any one of items 1-7, wherein the anti-redeposition composition further comprises one or more of a preservative, a solvent, a pH adjuster, and a detergent additive.

[0018] 9. A fabric detergent comprising the anti-redeposition composition described in any one of items 1-8.

[0019] 10. The fabric detergent according to item 9, wherein the content of the biosurfactant in the fabric detergent is 0.01wt%-50wt%.

[0020] 11. The fabric detergent according to claim 9 or 10, wherein the fabric detergent further comprises one or more of surfactants, detergent aids, preservatives, enzymes, pH adjusters, and fragrances.

[0021] 12. The fabric detergent according to claim 11, wherein the surfactant content is 5wt%-80wt%, the detergent additive content is 0-20wt%, the preservative content is 0.01wt%-20wt%, the enzyme content is 0.01wt%-1wt%, the pH adjuster content is 0.01wt%-1wt%, and the fragrance content is 0.01wt%-5wt%.

[0022] 13. The fabric detergent according to any one of claims 9-12, wherein the pH of the fabric detergent is 6-11, preferably 7.5-9.5.

[0023] 14. The fabric detergent according to any one of claims 9-13, wherein the fabric detergent is a liquid detergent, laundry pods, laundry powder, pre-wash agent, shoe cleaner or laundry soap.

[0024] 15. Uses of biosurfactants in fabric washing.

[0025] 16. The use according to item 15, wherein the biosurfactant acts as an anti-fouling agent during fabric washing.

[0026] 17. The use according to item 16, wherein the dirt is dirt washed off from a fabric.

[0027] 18. The anti-redeposition composition according to any one of items 15-17, wherein the biosurfactant is selected from one or more of lipopeptides, glycolipids and phospholipids.

[0028] 19. According to the use described in item 18, the lipopeptide is selected from one or more of the group consisting of: surfactants, iturobrine, fenbufen, lichenin, babylin, permiracin, spore-forming fungicides, antifungal fungicides, phosphatidylin, fusarium fungicides, kurstakin, spore-forming fungicides, polymyxins, octapeptide, and polypeptide fungicides.

[0029] 20. The use according to item 19, wherein the lipopeptide content in the fabric washing process is 0.01 g / L to 0.1 g / L.

[0030] 21. The use according to item 18, wherein the glycolipid is selected from one or more of the group consisting of: rhamnolipid, sophorolipid, mannose erythritol lipolipid, trehalose lipolipid and cellobiose lipolipid.

[0031] 22. According to the use described in item 21, the glycolipid content in the fabric washing process is 0.022 g / L to 0.1 g / L.

[0032] 23. The use according to item 18, wherein the phospholipid is selected from one or more of the group consisting of lecithin, cephalin, inositol phospholipid, serine phospholipid, and phosphorylethanolamine.

[0033] 24. The use according to item 23, wherein the phospholipid content in the fabric washing process is 0.006 g / L to 0.2 g / L.

[0034] 25. The use according to any one of items 15-24, wherein the biosurfactant is applied in the form of an anti-redeposition composition according to any one of items 1-8, or a fabric detergent according to any one of items 9-14.

[0035] This application provides a new use of biosurfactants in fabric washing. Biosurfactants or anti-redeposition compositions containing biosurfactants are produced by bacteria, are green-source raw materials, are harmless to the human body and environmentally friendly, are biodegradable, and have no harm with long-term use, which is in line with the development direction of green detergents. Biosurfactants not only have anti-redeposition function, but can also synergistically enhance the cleaning power of detergents by working with other surfactants during the fabric washing process. Attached Figure Description

[0036] Figure 1 Images of T-shirts washed with detergent 1 and detergent 2 in Example 5; Figure 2 The image shows a T-shirt washed with detergent 3 and detergent 4 in Example 5. Detailed Implementation

[0037] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.

[0038] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The present application is further described below with reference to specific embodiments, but is not intended to limit the scope of the application.

[0039] On the one hand, this application provides an anti-redeposition composition comprising 0.0001wt%-99.9999wt% of a biosurfactant as an active ingredient for preventing dirt redeposition.

[0040] In this application, "redeposition" and "secondary deposition" have the same meaning, referring to preventing or inhibiting the redeposition of dirt, for example, preventing or inhibiting the redeposition of dirt washed off a fabric.

[0041] In one specific embodiment, in the anti-redeposition composition, a biosurfactant is the sole active ingredient that inhibits the redeposition of dirt washed off the fabric. "Sole active ingredient" means that the anti-redeposition composition does not contain any other ingredients with anti-redeposition properties.

[0042] The biosurfactants of this application are surface-active metabolites produced by microorganisms during metabolism under specific culture conditions, including lipopeptides, glycolipids, and phospholipids.

[0043] The lipopeptides of this application may be cyclic or linear lipopeptides, preferably having one of the following formulas (I)-(III): (I) (II) (III) In this structure, R represents the carbon chain of the fatty acid chain, and A1, A2, A3…Am represent the 1st, 2nd, 3rd…mth amino acids on the peptide chain, respectively. The carboxyl group of the fatty acid is attached to the N-terminus of A1, and the C-terminal carboxyl group of Am is attached to the hydroxyl or amino groups of the fatty acid or other amino acids on the peptide chain to form a cyclic structure. Linear lipopeptides are linear products formed after the ring-opening of cyclic lipopeptides.

[0044] The lipopeptide can be any type of lipopeptide known in the art, such as those from the surfactant family, the iturobrine family, and the fenbufen (also known as fenbufen) family.

[0045] In one specific embodiment, the lipopeptide is selected from one or more of the following groups: surfactant, irisin, fenbufen, lichenin, babilin, peramitracin, spore-forming mycotoxin, antifungal mycotoxin, phosphatidylcholine, fusarium oxytocin, kurstakin, spore-forming mycotoxin, polymyxin, octapeptide, and polypeptide mycotoxin.

[0046] In one specific embodiment, the lipopeptide is a surfactant.

[0047] In one specific embodiment, the lipopeptide is itursin.

[0048] In one specific embodiment, the lipopeptide is fenestrated mustard.

[0049] The lipopeptide can be produced by bacteria, which can be wild-type or genetically engineered bacteria. For example, the bacteria can be selected from the group consisting of Bacillus, Pseudomonas, Streptomyces, and Arthrobacter.

[0050] In one specific embodiment, the lipopeptide is obtained by culturing Bacillus.

[0051] In one specific embodiment, the lipopeptide is obtained by culturing Pseudomonas bacteria.

[0052] In one specific embodiment, the lipopeptide is obtained by culturing Bacillus and regulating the expression of lipopeptide synthesis-related genes. These genes are selected from one or more of the following groups: lipopeptide synthesis gene srfA or fen (a fen-like substance), lic (a lichenin-like substance), bam (a spore-forming mycotoxin-like substance), mycotoxin-like substance, itu (a spore-forming mycotoxin-like substance), pps (a phosphatidylin-like substance), fusin (a fusin-like substance), krs (a spore-forming mycotoxin-like substance), pmx (a polymyxin-like substance), octapeptide-like substance, ycxA (a transmembrane transport protein-like substance), yngH (a biotin carboxylase-like substance), spore synthesis genes spoIVA / B / C / F, spoVA / B / D / E, and leucine synthesis pathway genes leuABCD / ilvK. The glycolipids of this application include various types of glycolipids produced by microbial fermentation, such as rhamnolipids, sophorolipids, and mannose-erythritol lipolipids.

[0053] In one specific embodiment, the glycolipid is selected from one or more of the following groups: rhamnolipid, sophorolipid, mannose erythritol lipolipid, trehalose lipolipid, and cellobiose lipolipid.

[0054] In one specific embodiment, the glycolipid is rhamnolipid.

[0055] In one specific embodiment, the glycolipid is sophorolipid.

[0056] The phospholipids in this application include various types of phospholipids produced by microbial fermentation, which are divided into two major categories: glycerol phospholipids and neurol phospholipids, such as lecithin, cephalin, inositol phospholipids, serine phospholipids, and phosphoryl ethanolamine.

[0057] In one specific embodiment, the phospholipid is phosphorylethanolamine.

[0058] Those skilled in the art will understand that the biosurfactants of this application can be obtained commercially or cultivated in-house. For example, the lipopeptides of this application can be produced by fermentation using Bacillus subtilis THY-7 / Pg3-srfA constructed in CN201810865295.7. Specifically, the fermentation broth obtained by directly fermenting Bacillus subtilis THY-7 / Pg3-srfA can be used as an anti-redeposition composition; or further, the pH of the fermentation broth can be adjusted to acidity, causing the lipopeptide surfactant to precipitate, which is then separated by solid-liquid separation and dried to obtain a product containing lipopeptide surfactant as an anti-redeposition composition. The content of surfactant in the composition can be above 70 wt%, for example, 70 wt%-99 wt%. The content of biosurfactant in the anti-redeposition composition of this application is 0.0001wt%-99.9999wt%, for example, it can be 0.0001wt%, 0.0002wt%, 0.0005wt%, 0.001wt%, 0.005wt%, 0.01wt%, 0.05wt%, 0.1wt%, 0.2wt%, 0.4wt%, 0.6wt%, 0.8wt%, 1.0wt%, 1.5wt%, 2.0wt%, 2.5wt%, 3.0wt%, 4.0wt%, 5.0wt%, 10wt%, 15wt%, 20wt%, 30wt%, 40wt%, 50wt%, 60wt%, 70wt%, 80wt%, 90wt%, 95wt%, 99wt%, 99.9999wt%, or any range between these values.

[0059] In one specific embodiment, the content of biosurfactant in the anti-redeposition composition is 70wt%-99wt%.

[0060] The anti-redeposition composition of this application may also include one or more of the following: preservatives, solvents, pH adjusters, and detergent additives.

[0061] The preservative can be selected from one or more of Kathon, DMDMH, MIT, and BIT.

[0062] The solvent may be selected from one or more of water, propylene glycol, glycerol, and ethanol, preferably water or propylene glycol. Preferably, the composition contains no more than 50 wt% of the solvent, preferably no more than 30 wt%, preferably no more than 20 wt%, and preferably no more than 5 wt%.

[0063] The pH of the anti-redeposition composition can be 6 to 11, preferably 7 to 10, and more preferably 7.0 to 9.0. Those skilled in the art can select suitable bases or suitable acids as pH adjusters to adjust the pH of the anti-redeposition composition. For example, suitable bases for adjusting the pH of the anti-redeposition composition include inorganic bases such as sodium hydroxide and potassium hydroxide; and organic bases such as monoethanolamine, diethanolamine, or triethanolamine; or 2-dimethylamino-2-methyl-1-propanol (DMAMP). Mixtures of bases may also be used. Suitable acids for adjusting the pH of aqueous media include inorganic acids such as hydrochloric acid, phosphoric acid, and sulfuric acid; and organic acids such as citric acid and acetic acid. Mixtures of acids may also be used.

[0064] When detergent additives are present in the anti-redeposition composition of this application, preferred detergent additives include citrates, phosphates, carbonates, aluminosilicates, organophosphonates, carboxylates, polycarboxylates, and polyacetylacetates. Detergent additives can be one or a mixture of two or more of these. Detergent additives can be added in the form of salts or acids. Preferably, the carbonates or citrates are sodium, potassium, or lithium salts. Preferred detergent additives include sodium carbonate, sodium bicarbonate, sodium citrate, or a mixture of two or more of these. Preferably, when detergent additives are present in the anti-redeposition composition of this application, their content, based on the total weight of the anti-redeposition composition, can be 0.1wt%-30wt%, preferably 0.5wt%-5wt%. The biosurfactants or anti-redeposition compositions containing biosurfactants of this application have good dispersing and emulsifying effects, as well as good cleaning power. They can not only reduce the redeposition of dirt during washing but also synergistically enhance the cleaning ability of the detergent with other surfactants in the detergent.

[0065] On the other hand, this application also provides a fabric detergent comprising any of the above-described anti-redeposition compositions.

[0066] In one specific embodiment, the content of the biosurfactant is 0.01wt%-50wt%, for example, it can be 0.01wt%, 0.05wt%, 0.1wt%, 0.2wt%, 0.4wt%, 0.6wt%, 0.8wt%, 1.0wt%, 1.5wt%, 2.0wt%, 2.5wt%, 3.0wt%, 4.0wt%, 5.0wt%, 6.0wt%, 7.0wt%, 8.0wt%, 9.0wt%, 10wt%, 15wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, 45wt%, 50wt%, or any range between these values. The fabric detergent may also include one or more of surfactants, detergent auxiliaries, preservatives, enzyme preparations, pH adjusters, and fragrances.

[0067] Regarding the specific types and amounts of surfactants, detergent auxiliaries, preservatives, enzyme preparations, pH adjusters, and fragrances, those skilled in the art can make corresponding adjustments based on the composition of existing fabric detergents and the specific form of the fabric detergent. Those skilled in the art will understand that the surfactants referred to here are surfactants other than the biosurfactants of this application.

[0068] For example, the surfactant can be one or more of anionic, nonionic, and amphoteric surfactants. Preferably, the fabric detergent contains at least one anionic surfactant, for example, it can contain one, two, or three anionic surfactants. The anionic surfactant can be any type of anionic surfactant known in the art, such as sulfonates, sulfates, carboxylates, etc. Preferably, the anionic surfactant has an alkyl group containing at least 10 carbon atoms and an anionic group, said anionic group preferably selected from sulfonate and sulfate groups. Some anionic surfactants are alkali metal salts of fatty acids. Preferably, the detergent composition contains 5 wt% to 20 wt% of a linear alkylbenzene sulfonate, preferably 8 wt% to 18 wt%, preferably 10 wt% to 18 wt%. Preferably, the alkylbenzene sulfonate has a C10-C14 alkyl group. Preferably, the detergent composition contains at least 3 wt% of a nonionic surfactant, preferably at least 5 wt%, preferably at least 6 wt%, preferably not more than 15 wt%, preferably not more than 12 wt%, preferably not more than 10 wt%. Nonionic surfactants are preferably polyoxyethylene type, alkyl glycoside type, polyol type, alkanolamide type, polyether type, or amine oxide type; zwitterionic surfactants are preferably betaine type or imidazoline type.

[0069] When a detergent auxiliary is present in the fabric detergent of this application, the detergent auxiliary may be selected from one or more of citrates, phosphates, carbonates, aluminosilicates, organophosphonates, carboxylates, polycarboxylates, and polyacetylacetates. The detergent auxiliary may be added in the form of a salt or an acid. Preferably, the carbonate or citrate is a sodium salt, potassium salt, or lithium salt. Preferred detergent auxiliaries are sodium carbonate, sodium bicarbonate, sodium citrate, or mixtures of two or more thereof.

[0070] The preservative can be one or a mixture of two or more of Kathon, DMDMH, MIT, and BIT.

[0071] Enzyme preparations may be one or a mixture of two or more of the following: protease, lipase, cellulase, and amylase.

[0072] The pH of fabric detergents can be 6-11, preferably 7-10, and more preferably 7.5-9.5. Those skilled in the art can select suitable alkalis or acids as pH adjusters to adjust the pH of fabric detergents. For example, suitable alkalis for adjusting the pH of detergents include inorganic alkalis such as sodium hydroxide and potassium hydroxide; and organic alkalis such as monoethanolamine, diethanolamine, or triethanolamine; or 2-dimethylamino-2-methyl-1-propanol (DMAMP). Mixtures of alkalis can also be used. Suitable acids for adjusting the pH of aqueous media include inorganic acids such as hydrochloric acid, phosphoric acid, and sulfuric acid; and organic acids such as citric acid and acetic acid. Mixtures of acids can also be used.

[0073] The fragrances used in this application may be fragrances known in the art that are suitable for use in fabric detergents.

[0074] In one specific embodiment, the fabric detergent contains 0.01wt%-50wt% of lipopeptides, 5wt%-80wt% of surfactants, 0-20wt% of detergent additives, 0.01wt%-20wt% of preservatives, 0.01wt%-1wt% of enzyme preparations, 0.01wt%-1wt% of pH adjusters, and 0.01wt%-5wt% of fragrances.

[0075] In one specific embodiment, the fabric detergent is composed of lipopeptides, surfactants, detergent auxiliaries, preservatives, enzyme preparations, pH adjusters, and fragrances. In the fabric detergent, the content of the lipopeptides is 0.01wt%-50wt%, the content of the surfactants is 5wt%-80wt%, the content of the detergent auxiliaries is 0-20wt%, the content of the preservatives is 0.01wt%-20wt%, the content of the enzyme preparations is 0.01wt%-1wt%, the content of the pH adjusters is 0.01wt%-1wt%, and the content of the fragrances is 0.01wt%-5wt%.

[0076] In one specific embodiment, the content of the glycolipid in the fabric detergent is 0.01wt%-50wt%, the content of the surfactant is 5wt%-80wt%, the content of the detergent additive is 0-20wt%, the content of the preservative is 0.01wt%-20wt%, the content of the enzyme preparation is 0.01wt%-1wt%, the content of the pH adjuster is 0.01wt%-1wt%, and the content of the fragrance is 0.01wt%-5wt%.

[0077] In one specific embodiment, the fabric detergent is composed of glycolipids, surfactants, detergent auxiliaries, preservatives, enzyme preparations, pH adjusters, and fragrances. In the fabric detergent, the content of glycolipids is 0.01wt%-50wt%, the content of surfactants is 5wt%-80wt%, the content of detergent auxiliaries is 0-20wt%, the content of preservatives is 0.01wt%-20wt%, the content of enzyme preparations is 0.01wt%-1wt%, the content of pH adjusters is 0.01wt%-1wt%, and the content of fragrances is 0.01wt%-5wt%.

[0078] Those skilled in the art will understand that the fabric detergents described in this application can encompass various forms (solid or liquid) of fabric washing compositions known in the art, such as laundry detergent, laundry pods, laundry powder, pre-wash agents, shoe cleaners, laundry soaps, etc.

[0079] On the other hand, this application also provides the use of biosurfactants in fabric washing.

[0080] In one specific embodiment, the biosurfactant acts as an anti-fouling agent during fabric washing. For example, the biosurfactant can inhibit the redeposition of dirt that has precipitated from the fabric.

[0081] The anti-redeposition effect can be tested by methods known in the art, such as visually observing the washing effect of the fabric before and after the addition of the biosurfactant, or by measuring the whiteness of the fabric after washing through a cyclic washing test.

[0082] The types and sources of the biosurfactants are as described above. When biosurfactants are used for fabric washing, the specific form of application is not limited. For example, biosurfactants can be applied to fabric washing in the form of the aforementioned anti-redeposition composition, or in the form of the aforementioned fabric detergent.

[0083] In one specific embodiment, the content of the biosurfactant in the fabric washing process is 0.0002 g / L-1 g / L, wherein the preferred content of lipopeptides in the fabric washing process is 0.01 g / L-0.1 g / L, the preferred content of glycolipids in the fabric washing process is 0.022 g / L-0.1 g / L, and the preferred content of phospholipids in the fabric washing process is 0.06 g / L-0.2 g / L.

[0084] This application provides novel uses of biosurfactants, including lipopeptides, glycolipids, and phospholipids, in fabric washing. Experimental results show that biosurfactants can effectively prevent secondary deposition of dirt during cyclic washing, and detergents containing biosurfactants can effectively remove dirt from fabrics. Furthermore, biosurfactants are harmless to humans, environmentally friendly, and biodegradable, aligning with the trend towards green detergents and thus possessing promising application prospects.

[0085] Example Example 1: Obtaining Biosurfactants 1. Obtaining compositions containing surfactants Single colonies of Bacillus subtilis THY-7 / Pg3-srfA (the construction method of THY-7 / Pg3-srfA is described in CN201810865295.7) were picked, inoculated into LB liquid medium, and cultured at 37℃ and 200rpm for 16 hours to obtain seed culture. The seed culture was then inoculated into a shake flask of fermentation medium at a ratio of 5%, and cultured at 37℃ and 200rpm for 2-6 hours. 1 Mm IPTG was added, and the culture was continued for 2 days to obtain the fermentation broth containing surfactant.

[0086] The fermentation medium used consisted of: 30-100 g / L sugars, 10-50 g / L inorganic nitrogen source, 0.5-3 g / L organic nitrogen source, 0.1-1 g / L KH2PO4, 0.5-0.3 g / L Na2HPO4·12H2O, 0.002-0.01 g / L CaCl2, 0.002-0.01 g / L MnSO4·H2O, 0.002-0.01 g / L FeSO4·7H2O, pH 6.5-7.5, and fermentation additives (silicone defoamers, polysaccharides, amino acids or their derivatives, oligopeptides, phospholipids, glycolipids, and / or fatty acids and their derivatives) 0.01-20 wt%.

[0087] The surfactant content in the fermentation broth was determined using the method disclosed in CN105400784A. Specifically, 1 mL of fermentation broth was centrifuged at 12000 rpm for 1 minute, and 100 μL of the supernatant was added to 1900 μL of deionized water. After mixing, the mixture was filtered through a 0.22 μm filter and analyzed by HPLC. The mobile phase for HPLC analysis was methanol and water in a ratio of 85 / 15, the flow rate was 1 mL / min, the chromatographic column was a C18-ODS reversed-phase column, the column temperature was 40℃, and the detection wavelength was 205 nm using a UV detector.

[0088] The content of surfactant in the fermentation broth was found to be approximately 10-50 g / L. Surfactant was extracted from the fermentation broth and obtained as a powder by freeze-drying. The content of lipopeptide in the obtained powder was 40 wt%-99.99 wt%. The powder was purified to obtain purified lipopeptide powder with a lipopeptide content of 95 wt%-99.99 wt%. The lipopeptide powder is composition 1 of this application.

[0089] 2. Obtaining a composition containing iturobrine and fenvalerate. Iridin and fenbufen were purchased from MedChemExpress and are used as Composition 2 and Composition 3 of this application, respectively. The lipopeptide content in Composition 2 and Composition 3 is 99wt%-99.99wt%.

[0090] 3. Obtaining glycolipid compositions Rhamnollipid and sophorollipid were purchased from Maclean's and are used as Composition 4 and Composition 5 of this application, respectively. The glycolipid content in Composition 4 and Composition 5 is 99 wt%-99.99 wt%.

[0091] 4. Obtaining phospholipid-containing compositions Phosphatidylethanolamine was purchased from MERCK and is used as composition 6 in this application. The phospholipid content in composition 6 is 99 wt%-99.99 wt%.

[0092] Example 2: Circulating Washing Test In this embodiment, compositions 1-6 from Example 1 were used to conduct cyclic washing tests, and detergent base formulations were prepared using the unit ratios described in Table 1 below.

[0093] Table 1 Detergent Base Formulation

[0094] Weigh 0.01 g of Composition 1 from Example 1 into 99.99 g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01 wt% Composition 1 for a cyclic washing test; continue to prepare detergents containing 0.02 wt%, 0.03 wt%, 0.04 wt%, 0.05 wt%, 0.06 wt%, 0.07 wt%, 0.08 wt%, 0.09 wt%, 0.10 wt%, 0.20 wt%, 0.30 wt%, 0.40 wt%, 0.50 wt%, 0.60 wt%, 0.70 wt%, 0.80 wt%, 0.90 wt%, 1.00 wt%, and 1.1 wt% of Composition 1 according to the above method. The detergent of Composition 1, in the following proportions (0 wt%, 1.20 wt%, 1.30 wt%, 1.40 wt%, 1.50 wt%, 1.60 wt%, 1.70 wt%, 1.80 wt%, 1.90 wt%, 2.00 wt%, 2.50 wt%, 3.00 wt%, 3.50 wt%, 4.00 wt%, 4.50 wt%, 5.00 wt%, 10.00 wt%, 15.00 wt%, 20.00 wt%, 25.00 wt%, 30.00 wt%, 35.00 wt%, 40.00 wt%, 45.00 wt%, 50.00 wt%), was subjected to a cyclic washing test.

[0095] Weigh 0.01g of Composition 2 from Example 1 into 99.99g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01wt% Composition 2, which was used for a cyclic washing test; the same method was continued to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, and 1.1wt%. The detergent of Composition 2, in the following proportions (0wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0096] Weigh 0.01g of Composition 3 from Example 1 into 99.99g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01wt% Composition 3 for a cyclic washing test; continue to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, and 1.1wt% of the above-mentioned composition 3. The detergent of composition 3, in the following proportions (0wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0097] Weigh 0.01g of Composition 4 from Example 1 into 99.99g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01wt% Composition 3, and use it for a cyclic washing test; continue to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, and 1.1wt% of the above-mentioned components according to the above method. The detergent of composition 3, in the following proportions (0wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0098] Weigh 0.01g of Composition 5 from Example 1 into 99.99g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01wt% Composition 3, and use it for a cyclic washing test; continue to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, and 1.1wt% of the above-mentioned compositions. The detergent of composition 3, in the following proportions (0wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0099] Weigh 0.01g of Composition 6 from Example 1 into 99.99g of the above-mentioned detergent base formulation to prepare a detergent containing 0.01wt% Composition 3, which was used for a cyclic washing test; the same method was continued to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, and 1.1wt%. The detergent of composition 3, in the following proportions (0wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0100] Weigh 0.01g of Composition 6 from Example 1 into 99.99g of the above-mentioned detergent base formulation, heat to dissolve, and prepare a detergent containing 0.01wt% Composition 3 for a cyclic washing test; continue to prepare detergents containing 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, 0.20wt%, 0.30wt%, 0.40wt%, 0.50wt%, 0.60wt%, 0.70wt%, 0.80wt%, 0.90wt%, 1.00wt%, 1 The detergent of composition 3, in the following proportions (10wt%, 1.20wt%, 1.30wt%, 1.40wt%, 1.50wt%, 1.60wt%, 1.70wt%, 1.80wt%, 1.90wt%, 2.00wt%, 2.50wt%, 3.00wt%, 3.50wt%, 4.00wt%, 4.50wt%, 5.00wt%, 10.00wt%, 15.00wt%, 20.00wt%, 25.00wt%, 30.00wt%, 35.00wt%, 40.00wt%, 45.00wt%, 50.00wt%), was subjected to a cyclic washing test.

[0101] The measurement conditions, preparations, and test pieces for the cyclic washing process are detailed in Table 2.

[0102] Table 2. Test conditions for cyclic washing

[0103] The determination of cyclic washing and whiteness retention was performed using the following method: Preparation of staining sheets: Cut JB00 white cotton cloth into 8cm×8cm squares, trim off the loose threads around the edges, and if necessary, pre-lock the edges with white cotton thread. The prepared test sheets are divided into groups of 6 for cyclic washing tests. Use WSB-2A whiteness meter to read the average whiteness value of each group of test sheets.

[0104] Preparation of hard water: In the circulating washing test, the detergent solution was prepared using 250 mg / kg hard water (calculated as CaCO3). 2 + With Mg 2+ With a molar ratio of 6:4, weigh 16.70g of calcium chloride and 20.37g of magnesium chloride, add deionized water to prepare 10.0L of water, which is 2500mg / kg hard water. When using, take 100mL and dilute it with deionized water to 1L to prepare 250mg / kg hard water.

[0105] Preparation of detergent solution: Weigh 2g of the detergent base formulation described in Table 1, use 250mg / kg of hard water to prepare 1L of detergent solution with a concentration of 2g / L, and adjust the pH value to 8.

[0106] Vertical decontamination testing machine with a rotation speed range of 30 r / min to 200 r / min, Anti-redeposition performance was evaluated in a 120mm high, 170mm high cleaning bath. Before testing, the stirring impeller, working tank, and cleaning bath were numbered and fixed to form a "working unit". During the test, 1L of the prepared 2g / L washing solution was poured into the corresponding cleaning bath, the bath was placed in the corresponding position and the stirring impeller was installed. The instrument was adjusted to maintain the washing test temperature at (30±1)℃.

[0107] Add 3 mL of JB-W1 waste liquid to each bathtub and start stirring for 30 seconds.

[0108] Each group of test pieces was placed in its respective cleaning bath and washed at 120 rpm for 30 minutes. After washing, the pieces were removed, rinsed, and allowed to air dry to complete one wash cycle. The washed test pieces were then washed repeatedly, with the whiteness retention calculated after 20 consecutive washes.

[0109] The experimental results are shown in Tables 3-5.

[0110] Table 3 Results of the cyclic washing test of Composition 1

[0111] Table 4 Results of the cyclic washing test of Composition 2

[0112] Table 5 Results of the cyclic washing test of composition 3

[0113] Table 6 Results of the cyclic washing test of composition 4

[0114] Table 7 Results of the cyclic washing test of composition 5

[0115] Table 8 Results of the cyclic washing test of composition 6

[0116] The experimental results above show that after 20 cycles of washing, the whiteness retention rate of the cotton white cloth with added compositions 1-6 of Example 1 is significantly higher than that of the white cloth without the added compositions. This indicates that compositions 1-6 in Example 1 have good anti-redeposition properties and can effectively prevent secondary deposition of dirt during repeated washing. The anti-redeposition effect of the above compositions is positively correlated with the amount added within a certain range. When the amount of compositions 1, 2, and 3 added is greater than or equal to 0.50 wt%, the whiteness retention rate is increased by more than 10% compared to the white cloth without the added compositions. However, when the amount of compositions 1, 2, and 3 added is greater than 5.00 wt%, the increase in whiteness value with increasing addition is relatively small. Therefore, the preferred content of lipopeptides during the washing process is 0.01-0.1 g / L. The preferred addition amount of compositions 1, 2, and 3 is 0.50 wt% to 5.00 wt%. When the addition amount of compositions 4 and 5 is greater than or equal to 1.10 wt%, the whiteness retention rate is increased by more than 10% compared to the composition without addition. However, when the addition amount of composition 4 is greater than 5.00 wt%, the increase in whiteness retention rate with increasing addition amount is small. Therefore, the content of glycolipids in the washing process is preferably 0.022-0.1 g / L. The preferred addition amount of compositions 4 and 5 is 1.10 wt% to 5.00 wt%. When the addition amount of composition 6 is greater than 3.0 wt%, the whiteness retention rate is increased by more than 10% compared to the composition without addition. However, when the addition amount of composition 6 is greater than 10.00 wt%, the increase in whiteness retention rate with increasing addition amount is small. Therefore, the content of phospholipids in the washing process is preferably 0.06 g / L to 0.2 g / L. The preferred addition amount of composition 6 is 3.0 wt% to 10.0 wt%.

[0117] Example 3: Fabric detergent with anti-redeposition function and its preparation method This embodiment utilizes composition 1 from Example 1 to prepare a fabric detergent with anti-redeposition function. Its raw material composition is shown in Table 9.

[0118] Table 9 Raw materials and composition of fabric detergents with anti-redeposition function

[0119] The preparation method is as follows: 1. Add 50% deionized water and EDTA2Na to the stirred tank and stir until well mixed; 2. Add sodium linear alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether, sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine, and alkyl glycoside to a mixing tank and stir thoroughly until homogeneous; 3. Add an appropriate amount of sodium hydroxide to adjust the pH to 7.0-8.5; 4. Add Composition 1 of Example 1, flavoring, sodium chloride, and preservative, and stir until well mixed; 5. Add the remaining deionized water to bring the total to 100%; 6. Once the sample passes the inspection, the material can be discharged.

[0120] The fabric detergent with added anti-redeposition composition in this embodiment is pale yellow, transparent, and free of suspended or precipitated impurities, and has no odor other than added fragrance. After being placed in temperature (40℃±2℃) and cold (-5℃±2℃) tests, the product showed no change in color or odor, and no suspended matter, sediment, or crystallization. All product indicators meet the relevant national or industry standards.

[0121] Example 4: Laundry detergent pods with anti-redeposition function and their preparation method This embodiment uses composition 4 from Example 1 to prepare a laundry detergent pod with anti-redeposition function.

[0122] Its raw material composition is shown in Table 10.

[0123] Table 10 Raw materials and composition of laundry detergent pods with anti-redeposition function

[0124] The preparation method is as follows: 1. Add propylene glycol, cocamidopropyl betaine, and alkyl glycoside to the mixing vessel and stir until homogeneous; 2. Add sodium linear alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether, and sodium fatty alcohol polyoxyethylene ether sulfate, and stir thoroughly until clear and transparent; 3. Add EDTA2Na, preservative, enzyme preparation, composition 4 of Example 1, and flavoring, and stir well; 4. Add an appropriate amount of sodium hydroxide to adjust the pH to 7.0-8.5; 5. Add glycerin to 100 and stir thoroughly until clear and transparent; 6. After the sample passes the inspection, the material is discharged and filled into a water-soluble film mold to obtain laundry detergent beads with anti-redeposition function.

[0125] The laundry detergent pods of this embodiment, containing an anti-redeposition composition, are clear and transparent, free of suspended or precipitated impurities, and have no odor other than added fragrance. After being placed in temperature (40℃±2℃) and cold (-5℃±2℃) tests, the product showed no change in color or odor, no stratification, no suspended matter or sediment, and no crystallization. All product indicators meet the relevant national or industry standards.

[0126] Example 5: Detergent performance test of fabric containing lipopeptide composition on fabrics. This embodiment tests the effects of the fabric detergents in Examples 3 and 4 on fabrics after a single wash and 20 washes, and compares them with the washing performance of the detergent without the composition in Example 1.

[0127] Detergent 1 and Detergent 2 were prepared according to the methods in Examples 3 and 4, respectively. Compositions 1 and 4 in the formulations and processes of Examples 3 and 4 were removed to prepare Detergent 3 and Detergent 4 without anti-redeposition compositions. Four identical brand-new cotton white T-shirts were taken and washed with Detergent 1, 2, 3 and 4 respectively under the conditions in the table below.

[0128] Table 11 Washing performance test conditions

[0129] *Preparation of artificial dirt: Mix edible vegetable oil, butter, whole milk, whole egg liquid, and vegetable charcoal black in a weight ratio of 1:1:1:1:1, stir well and set aside.

[0130] Wash the T-shirt once according to the washing conditions described above, and then air dry it naturally under the same conditions (in a cool, dark place).

[0131] The T-shirts washed with detergent 1 and detergent 2 showed no significant color change, no visible dirt deposits on the fabric, and felt soft to the touch, without any graying, dullness, or roughness. The T-shirts washed with detergent 3 and detergent 4 showed a small amount of dirt deposits on the surface, with noticeable yellow and black clumps of dirt.

[0132] Continue washing the T-shirt using the above method for 20 cycles. After washing with detergent 1 and detergent 2, the overall color of the T-shirt is white, with a small amount of dirt deposited on the surface and some yellow and black stains. The garment does not show any obvious graying, dullness, or roughness (e.g., Figure 1 As shown, where Figure 1 The left image shows the washing results of detergent 1. Figure 1 The right image shows the washing results of detergent 2; after washing with detergents 3 and 4, the T-shirt is generally gray and yellowish, with a large number of yellow and black stains (such as...). Figure 2 As shown, where Figure 2 The left image shows the washing results of detergent 3. Figure 2 The right figure shows the washing results of detergent 4), and the softness decreased significantly, the fabric became stiff, and the feel was rough. Therefore, it can be seen that the fabric detergents in Examples 3 and 4 have good anti-redeposition properties, that is, the detergents prepared using the anti-redeposition composition of this application have good anti-redeposition function.

Claims

1. Use of a biosurfactant in anti-redeposition of dirt during fabric washing, wherein the biosurfactant is the sole active ingredient for anti-redeposition of dirt, the dirt being dirt washed off the fabric, and the biosurfactant is selected from one or both of lipopeptides and phospholipids.

2. The use according to claim 1, wherein the lipopeptide is selected from one or more of the following groups: surfactant, ituronin, fenbufen, lichenin, babylin, permiracin, spore-forming mycotoxin, antifungal mycotoxin, phosphatidylcholine, fusarium oxytocin, kurstakin, spore-forming mycotoxin, polymyxin, octapeptide, and polypeptide mycotoxin.

3. The use according to claim 1, wherein the content of the lipopeptide in the fabric washing process is 0.01g / L-0.1g / L.

4. The use according to claim 1, wherein the phospholipid is selected from one or more of the group consisting of lecithin, cephalin, inositol phospholipid and serine phospholipid.

5. The use according to claim 4, wherein the phospholipid content in the fabric washing process is 0.06 g / L-0.2 g / L.

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