Application of Osmanthus fragrans OfYABBY12 gene in reducing β-ionone content in Nicotiana tabacum
By transforming the osmanthus OfYABBY12 gene in Dahua Tobacco, the morphology and aroma characteristics of the plant are changed, and the problem of difficulty in reducing the β-ionone content in the prior art is solved, and the ornamentality and economic value of Dahua Tobacco is improved.
CN117004619BActive Publication Date: 2024-12-20NANJING HOUDE BIOTECHNOLOGY CO LTD
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Patent Information
- Application Number
- CN202310918004.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-25
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2043-07-25
AI Technical Summary
Technical Problem
The prior art is difficult to effectively reduce the content of β-ionone in large-flower tobacco, affecting the ornamentality and aroma characteristics of plants.
Method used
By cloning the osmanthus OfYABBY12 gene, the GFP::pCAMBIA1300-OfYABBY12 vector was constructed and transformed into large-flower tobacco, resulting in leaf shrinkage, increased flower pistil length, decreased leaf thickness, and changes in terpene biosynthetic gene expression, significantly reducing β -Uionone content.
Benefits of technology
It successfully reduced the β-ionone content in large-flower tobacco, changed the morphology and aroma characteristics of the plant, and significantly improved the ornamentality and economic value of the plant.
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Figure CN117004619B_ABST
Abstract
The invention discloses the application of the sweet osmanthus OfYABBY12 gene in reducing the content of β-ionone in Nicotiana tabacum, and belongs to the field of plant molecular biology. The invention uses the flowers of the osmanthus fragrans in full bloom as materials, obtains the sweet osmanthus OfYABBY12 gene by cloning, constructs its overexpression vector GFP::pCAMBIA1300-OfYABBY12 on this basis, transfers it into Nicotiana tabacum, and obtains transgenic plants. The mature leaves of the transgenic Nicotiana tabacum leaves are obviously curled downward and wrinkled, and the length of the pistil (style) of the flower is obviously elongated compared with the wild-type Nicotiana tabacum, and the leaf thickness is reduced by 33%, 33%, and 57% respectively compared with the wild-type. The expression levels of the terpene biosynthesis difference genes DXS, MCT, CMK, HMGS, HMGR, MVK, PMK, and MVD in the transgenic Nicotiana tabacum are significantly upregulated, and the expression level of the CCD gene is significantly downregulated.
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