A hair loss prevention composition, its preparation method and application

Abstract

This invention discloses a hair loss prevention composition, its preparation method, and its application, relating to the daily chemical industry. The hair loss prevention composition comprises, by mass percentage: 25%-50% *Pinus marisonii* bark extract, 45%-70% *Tea styrosine* extract, and 0.01%-5% dihydrosphingosine. Through this compounding process, it can effectively reduce oxidative stress damage to hair follicles, protect hair follicles, soothe skin inflammation, regulate microcirculation, promote hair regeneration and dermal papilla cell proliferation, thereby helping to prevent or treat androgenetic alopecia, inhibiting 5α-reductase in sebum and hair follicles, and prolonging the hair growth phase, reducing oiliness and preventing hair loss. The composition exhibits good light and heat stability and mildness. These three components work synergistically, and when applied to cosmetics, they not only effectively reduce hair loss but also soothe scalp irritation, strengthen the scalp barrier, and possess good stability.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products, and more specifically, to a hair loss prevention composition, its preparation method, and its application. Background Technology

[0002] Hair, the hair that grows on the head, not only protects the scalp, shields against ultraviolet rays, and regulates temperature, but also greatly enhances our appearance. Hair loss can severely impact our image, and with rapid societal progress, the number of people experiencing hair loss due to factors such as high stress and irregular eating and sleeping habits is increasing and showing a trend towards affecting younger people. The desire for beauty is universal, and hair loss has become a widely discussed and pressing issue.

[0003] Currently, topical ingredients used to treat hair loss mainly include synthetic chemical components and some specific plant extracts. The range of chemical ingredients used to treat hair loss is still relatively limited. Among them, minoxidil, which is widely used and has good efficacy, has certain side effects, and long-term use may lead to drug dependence; once discontinued, hair loss may worsen. On the other hand, some herbal extracts used to treat hair loss often have complex and unstable compositions due to differences in plant sources and extraction processes, which can also lead to safety concerns.

[0004] Patent application CN 114588082 A discloses a herbal shampoo that not only alleviates dandruff and hair loss but also effectively improves hair quality and nourishes the scalp, characterized by its gentle and non-irritating properties. However, on the one hand, the anti-hair loss effect of the shampoo prepared by the aforementioned patent is unclear; on the other hand, although the added pine extract can protect hair from excessive ultraviolet radiation and free radical damage, its antioxidant component, proanthocyanidins, is an unstable substance that is easily degraded by factors such as light and high temperature, ultimately affecting the stability and efficacy of the product.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The purpose of this invention is to provide a hair loss prevention composition, its preparation method, and its application.

[0007] This invention is implemented as follows:

[0008] In a first aspect, the present invention provides a hair loss prevention composition, the components of which, by mass percentage, comprise: 25%-50% of Maritime Pine Bark Extract, 45%-70% of Tea Extract, and 0.01%-5% of Dihydrosphingosine.

[0009] In an optional embodiment, the components, by mass percentage, include: 30%-45% of Maritime Pine Bark Extract, 50%-65% of Tea Extract, and 3%-5% of Dihydrosphingosine.

[0010] Secondly, the present invention provides a method for preparing a hair loss prevention composition as described in any of the foregoing embodiments, wherein the components are mixed evenly.

[0011] In an optional embodiment, the mixing includes stirring at 200-400 rpm / min for 4-6 min.

[0012] Thirdly, the present invention provides the use of the hair loss prevention composition as described in any of the foregoing embodiments in the preparation of hair loss prevention cosmetics or anti-irritant cosmetics.

[0013] In an optional embodiment, the anti-hair loss cosmetic includes scalp care products or hair cleansing products, wherein the scalp care products include one or more of scalp serums, conditioners, and hair masks; and the hair cleansing products include shampoos.

[0014] Fourthly, the present invention provides a scalp care product for preventing hair loss, the components of which, by weight, include: 0.1-40% oil, 0.1-5% emulsifier, 0.01-2% thickener, 3-10% moisturizer, 0.1-1% preservative, 0.02-0.1% disodium EDTA, 0.1-10% hair loss prevention composition as described in any of the foregoing embodiments, and the balance being deionized water.

[0015] In an optional embodiment, the oil comprises one or more of the following: isopropyl myristate, polydimethylsiloxane, caprylic / capric triglyceride, dioctyl carbonate, hydrogenated polyisobutylene, shea butter, ethylhexyl palmitate, hydrogenated polydecene, isononyl isononanoate, cetyl ethylhexyl, phytosterols, unsaponifiables of olive oil, jojoba seed oil, squalane, rosemary leaf oil, lavender oil, sweet orange oil, sunflower seed oil, and camellia seed oil.

[0016] Preferably, the emulsifier comprises one or more of the following: cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, polyglycerol-10 stearate, glyceryl stearate / PEG-100 stearate, sorbitan sesquioleate, isostearyl alcohol polyether-20, PEG-10 rapeseed sterol, lauryl alcohol polyether-7, polyglycerol-3 methyl glucoside distearate, polyglycerol-3 diisostearate, glyceryl stearate, sucrose stearate, stearyl alcohol polyether-20, cetearyl alcohol polyether-20, polysorbate-60, hydrogenated lecithin, and soybean (Glycine soja) seed extract;

[0017] Preferably, the thickener includes one or more of the following: carbomer, sodium carbomer, xanthan gum, carrageenan, gellan gum, sclerotinia gum, sodium polyacrylate, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, acrylate copolymers, and polyacrylate crosspolymer-6.

[0018] Preferably, the moisturizer comprises one or more of the following: glycerin, diglyceride, glyceryl glucoside, propylene glycol, 1,3-propanediol, butylene glycol, panthenol, sorbitol, betaine, sodium PCA, zinc PCA, hydroxyethyl urea, sodium hyaluronate, and β-glucan.

[0019] Preferably, the preservative includes one or more of phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, methylparaben, p-hydroxyacetophenone, 1,2-hexanediol, and octyl glycol.

[0020] Fifthly, the present invention provides a method for preparing an anti-hair loss scalp care product as described in the foregoing embodiments, wherein the components are mixed evenly.

[0021] In an optional embodiment, mixing the components uniformly includes:

[0022] The emulsifier and the oil are mixed, heated to 75-85°C, and kept at that temperature for 1-20 minutes until the raw materials are completely dissolved to obtain phase A.

[0023] The thickener, disodium EDTA and water are mixed and homogenized for 1-5 minutes. Then, the mixture is heated to 75-85°C and kept at that temperature for 5-30 minutes. The mixture is stirred thoroughly until the material is completely dissolved to obtain phase B.

[0024] After adding phase A to phase B and homogenizing for 3-10 minutes, stir and cool.

[0025] When the temperature drops to below 45°C, add the preservative, the moisturizer, and the hair loss prevention composition, mix well, and then discharge.

[0026] The present invention has the following beneficial effects:

[0027] The hair loss prevention composition provided in this application comprises a compound of Maritime Pine Bark Extract, Tea Extract, and Dihydrosphingosine. Maritime Pine Bark Extract, through its strong free radical scavenging activity, reduces oxidative stress damage to hair follicles, protects hair follicles, soothes skin inflammation, regulates microcirculation, and promotes hair regeneration. Tea Extract neutralizes free radicals and possesses excellent antioxidant capacity. Furthermore, the tea polyphenols in Tea Extract inhibit 5α-reductase activity and promote the proliferation of dermal papilla cells, thereby helping to prevent or treat androgenetic alopecia. Dihydrosphingosine can improve scalp barrier function by inducing differentiation and ceramide production through keratinization of keratinocytes. In addition, Dihydrosphingosine also inhibits 5α-reductase in sebum and hair follicles and prolongs the hair growth phase, reducing oiliness and preventing hair loss. Furthermore, tea extract can effectively protect the coastal pine bark extract, resulting in good light and heat stability in the prepared composition. Dihydrosphingosine can synergistically enhance the efficacy and mildness of the composition with the coastal pine bark extract and tea extract. These three components can work synergistically. When applied in cosmetics, this composition can not only effectively reduce hair loss but also soothe scalp irritation, strengthen the scalp barrier, and exhibit good stability. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0029] This invention provides a hair loss prevention composition, the components of which, by mass percentage, comprise: 25%-50% of Maritime Pine Bark Extract, 45%-70% of Tea Extract, and 0.01%-5% of Dihydrosphingosine.

[0030] Maritime pine, a plant that thrives in strong winds and sunlight, contains bark extracts rich in the active substance proanthocyanidins (OPC). These OPCs not only reduce oxidative stress damage to hair follicles through their strong free radical scavenging activity, protecting them, but also soothe skin inflammation by inhibiting the release of inflammatory mediators IL8 and IL6. When used in hair products, they can also protect hair from excess UV radiation and free radical damage. Furthermore, maritime pine bark extract contains various bioactive components, such as flavonoids, polysaccharides, caffeic acid, ferulic acid, gallic acid, and vanillic acid, which can regulate microcirculation, inhibit sebum secretion, and promote hair regeneration.

[0031] The addition amount of coastal pine bark extract is 25-50%, preferably 30-45%, for example, it can be any one of 26%, 27%, 30%, 33%, 38%, 40%, 46%, 49%, 50%, or any range between two of these. The addition amount of coastal pine bark extract at 25-50% can produce a synergistic effect with tea extract and dihydrosphingosine, thereby enhancing the antioxidant stability of the coastal pine bark extract in cosmetic applications and providing superior anti-hair loss and anti-irritation capabilities.

[0032] Tea extracts are rich in active substances such as tea polyphenols and catechins, which can neutralize free radicals and have excellent antioxidant capabilities. Furthermore, tea polyphenols in tea extracts can inhibit the activity of 5α-reductase and promote the proliferation of dermal papillary cells, thus helping to prevent or treat androgenetic alopecia.

[0033] The amount of tea extract added is 45-70%, preferably 50-65%, for example, it can be any one of 47%, 50%, 55%, 60%, 61%, 64%, 69%, or any range between two of these. Adding 45-70% tea extract can significantly improve the light and heat stability of the coastal pine bark extract in cosmetic applications, maintain its excellent antioxidant activity, and also enhance the anti-hair loss efficacy of the composition.

[0034] Dihydrosphingosine can enhance scalp barrier function by inducing differentiation and ceramide production through keratinocyte differentiation. Furthermore, dihydrosphingosine also inhibits 5α-reductase in sebum and hair follicles and prolongs the hair growth phase, thus reducing oiliness and preventing hair loss.

[0035] The amount of dihydrosphingosine added is 0.01-5%, preferably 3-5%, for example, it can be any one of 0.02%, 0.05%, 0.1%, 0.5%, 1%, 1.7%, 2%, 3%, 4%, 4.8% or any range between two of these. Adding 0.01-5% of dihydrosphingosine enhances the anti-hair loss properties of the coastal pine bark extract, improves the mildness and anti-irritant properties of the composition, and also provides better product applicability.

[0036] Furthermore, this application can also define the above-mentioned coastal pine bark extract, tea extract and dihydrosphingosine by weight ratio, wherein the weight ratio of coastal pine bark extract, tea extract and dihydrosphingosine in the composition is 1:(0.9-2.8):(0.0002-0.2); preferably 1:(1.1-2.2):(0.1-0.2).

[0037] The preparation method of the above-mentioned anti-hair loss composition is simple. It only requires stirring the above-mentioned coastal pine bark extract, tea extract and dihydrosphingosine at 200-400 rpm / min for 4-6 minutes until they are mixed evenly.

[0038] The hair loss prevention composition prepared in this application can be widely used in various hair loss prevention cosmetics or anti-irritation cosmetics. Hair loss prevention cosmetics may include, for example, scalp care products or hair cleansing products. Scalp care products include, but are not limited to, one or more of scalp serums, conditioners, and hair masks; hair cleansing products include, but are not limited to, shampoos. Anti-irritation cosmetics may include, for example, products with irritating properties, in which the above-mentioned hair loss prevention composition can be added to improve irritation.

[0039] For example, the present invention also provides a scalp care product for preventing hair loss, the components of which, by weight, include: 0.1-40% oil, 0.1-5% emulsifier, 0.01-2% thickener, 3-10% moisturizer, 0.1-1% preservative, 0.02-0.1% disodium EDTA, 0.1-10% hair loss prevention composition, and the balance being deionized water.

[0040] The oils include one or more of the following: isopropyl myristate, polydimethylsiloxane, caprylic / capric triglyceride, dioctyl carbonate, hydrogenated polyisobutylene, shea butter, ethylhexyl palmitate, hydrogenated polydecene, isononyl isononanoate, cetyl ethylhexyl, phytosterols, unsaponifiables of olive oil, jojoba seed oil, squalane, rosemary leaf oil, lavender oil, sweet orange oil, sunflower seed oil, and camellia seed oil.

[0041] The emulsifiers include one or more of the following: cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, polyglycerol-10 stearate, glyceryl stearate / PEG-100 stearate, sorbitan sesquioleate, isostearyl alcohol polyether-20, PEG-10 rapeseed sterol, lauryl alcohol polyether-7, polyglycerol-3 methyl glucoside distearate, polyglycerol-3 diisostearate, glyceryl stearate, sucrose stearate, stearyl alcohol polyether-20, cetyl alcohol polyether-20, polysorbate-60, hydrogenated lecithin, and soybean (Glycine soja) seed extract;

[0042] Thickeners include one or more of the following: carbomer, sodium carbomer, xanthan gum, carrageenan, gellan gum, sclerotinia gum, sodium polyacrylate, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, acrylate copolymers, and polyacrylate crosspolymer-6.

[0043] Moisturizers include one or more of the following: glycerin, diglycerin, glyceryl glucoside, propylene glycol, 1,3-propanediol, butylene glycol, panthenol, sorbitol, betaine, sodium PCA, zinc PCA, hydroxyethyl urea, sodium hyaluronate, and beta-glucan.

[0044] Preservatives include one or more of phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, methylparaben, p-hydroxyacetophenone, 1,2-hexanediol, and octyl glycol.

[0045] The preparation method of the above-mentioned anti-hair loss scalp care products includes:

[0046] Mix the emulsifier and oil, heat to 75-85℃, keep warm for 1-20 minutes, and obtain phase A after the raw materials are completely dissolved;

[0047] Mix the thickener, disodium EDTA, and water, homogenize for 1-5 minutes, heat to 75-85℃, keep warm for 5-30 minutes, and stir thoroughly until the mixture is completely dissolved to obtain phase B.

[0048] Add phase A to phase B and homogenize for 3-10 minutes, then stir and cool.

[0049] When the temperature drops below 45°C, add preservatives, humectants, and hair loss prevention composition, mix well, and then discharge.

[0050] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0051] Examples 1-8

[0052] All raw material components of this invention are commercially available products. Among them, the coastal pine bark extract was purchased from Guangzhou Fuwei Biotechnology Co., Ltd., the tea extract was purchased from Guangzhou Aoxue Chemical Co., Ltd., and the dihydrosphingosine was purchased from Guangzhou Hefu Chemical Co., Ltd.

[0053] Prepare the composition samples of each embodiment according to the proportions in Table 1. Add each component to a clean container in sequence at room temperature and stir at 300 rpm / min for 5 minutes to mix evenly to obtain the hair loss prevention composition. Store in a sealed container at room temperature for later use.

[0054] Table 1. Components of the compositions in Examples 1-8 (unit: weight percentage %)

[0055] Example Coastal pine bark extract Tea extract Dihydrosphingosine Example 1 25 70 5 Example 2 35 60 5 Example 3 45 50 5 Example 4 50 45 5 Example 5 30 67 3 Example 6 40 59 1 Example 7 50 49.9 0.1 Example 8 45 54.99 0.01

[0056] Comparative Examples 1-7

[0057] Prepare the comparative composition samples according to the proportions in Table 2. Add each component to a clean container in the proportions at room temperature, and stir at 300 rpm / min for 5 minutes to mix evenly to obtain the hair loss prevention composition. Store in a sealed container at room temperature for later use.

[0058] Table 2. Component list of each composition in Comparative Examples 1-7 (unit: weight percentage %)

[0059] Example Coastal pine bark extract Tea extract Dihydrosphingosine Grape seed extract Comparative Example 1 0 95 5 0 Comparative Example 2 40 60 0 0 Comparative Example 3 60 40 0 0 Comparative Example 4 95 0 5 0 Comparative Example 5 75 20 10 0 Comparative Example 6 15 80 10 0 Comparative Example 7 0 70 5 25

[0060] Application Examples 1-8, Comparative Examples 1-7, and Control Group

[0061] The anti-hair loss composition samples of Examples 1-8 were added to the emulsion formulations in Table 3 at a mass fraction of 5%; the anti-hair loss composition samples of Comparative Examples 1-7 were added to the emulsion formulations in Table 4 at a mass fraction of 5%, with the formulations without the anti-hair loss composition serving as the control group. Sixteen emulsions were prepared according to the following method.

[0062] The steps for preparing the emulsion are as follows:

[0063] 1) Mix the A phase raw materials, heat to 75-85℃, stir to dissolve evenly, and keep warm for 5 minutes;

[0064] 2) After mixing the B phase raw materials, homogenize for 3 minutes, heat to 75-85℃, keep warm for 20 minutes, and stir thoroughly until the material is completely dissolved;

[0065] 3) Add phase A to phase B, homogenize for 5 minutes, then stir and cool.

[0066] 4) After the entire system has been continuously stirred and cooled to below 45°C, add phase C and phase D, stir evenly, and then discharge the material;

[0067] Table 3. Emulsion composition of Application Examples 1-8 (unit: weight percentage %)

[0068]

[0069]

[0070] Table 4. Emulsion composition of Comparative Examples 1-7 and the control group (unit: weight percentage %)

[0071]

[0072] Performance testing

[0073] (1) Stability test

[0074] Fifteen emulsion samples containing the anti-hair loss compositions of Examples 1-8 and Comparative Examples 1-7, as well as a control group emulsion without the anti-hair loss compositions, were placed in transparent PET bottles for high-temperature, outdoor light, and xenon lamp light stability tests. Their appearance was observed, and the test results are shown in Table 5 below.

[0075] Test conditions:

[0076] High-temperature accelerated test: 48℃±1℃, 25%RH±5%RH for 1 month.

[0077] Outdoor light exposure test: Placed under natural light for 10 days.

[0078] Xenon lamp illumination test: illumination intensity 0.35W / m2, test for 26.4 hours, blackboard temperature 45℃ (not chamber temperature), humidity 50%.

[0079] Table 5. Statistical table of stability test results of emulsions prepared for different application examples

[0080] Application Examples initial state high temperature Natural light Xenon lamp illumination Application Example 1 Light red lotion normal normal normal Application Example 2 Light red lotion normal normal normal Application Example 3 Red lotion normal normal normal Application Example 4 Red lotion normal normal normal Application Example 5 Light red lotion normal normal normal Application Example 6 Red lotion normal normal normal Application Example 7 Red lotion normal normal normal Application Example 8 Red lotion normal normal normal Application Comparative Example 1 off-white emulsion normal normal normal Application Comparative Example 2 Red lotion normal normal normal Application Comparative Example 3 Red lotion Color lightens Color lightens Color lightens Application Comparative Example 4 Reddish-brown emulsion fade fade fade Application Comparative Example 5 Red lotion Fading, particle precipitation Fading, particle precipitation Fading, particle precipitation Application Comparative Example 6 Light red emulsion Particle precipitation Particle precipitation Particle precipitation Application Comparative Example 7 Light red lotion normal normal normal control group off-white emulsion normal normal normal

[0081] As can be seen from Examples 1-8, adding an appropriate concentration of tea extract can effectively protect the coastal pine bark extract, resulting in good light and heat stability of the prepared compositions. The role of dihydrosphingosine in the compositions of this invention is mainly to synergistically enhance the efficacy and mildness of the compositions with the coastal pine bark extract and tea extract. When its addition amount is in the concentration range of 0.01-5%, it has no significant effect on the light and heat stability of the emulsion.

[0082] The emulsion prepared in the control group did not contain the anti-hair loss composition of the present invention, and its appearance did not change under high temperature and light conditions.

[0083] The emulsion prepared using Comparative Example 1, which did not contain any Maritime Pine Bark Extract, also exhibited good photothermal stability.

[0084] Although no dihydrosphingosine was added in Comparative Example 2, the emulsion sample prepared by the other two active ingredients was also free from abnormalities under high temperature and light conditions because they were within the concentration range of the present invention.

[0085] In Comparative Example 3, the emulsion lightened in color under high temperature and light conditions. This was because the extracts of coastal pine bark and tea leaves were not within the scope of this invention, and the concentration of the tea leaves extract was too low, which caused the coastal pine bark extract to degrade to a certain extent.

[0086] In Comparative Example 4, the lack of tea extract to protect the coastal pine bark extract led to its complete degradation under high temperature and light conditions, resulting in fading.

[0087] The emulsion prepared in Comparative Example 5 faded for the same reason as above. Furthermore, because the amount of dihydrosphingosine added to the composition was too high, reaching 10%, dihydrosphingosine is difficult to dissolve without sufficient polyols or oils. Therefore, in the emulsion system of this invention, the large amount of dihydrosphingosine added leads to emulsion instability and particle precipitation.

[0088] In Comparative Example 6, the cause of particle precipitation in the emulsion was the same as in Comparative Example 5.

[0089] In Comparative Example 7, since grape seed extract also contains the active substance proanthocyanidins, the presence of tea extract can also protect it, thus obtaining an emulsion with good stability.

[0090] (2) Anti-hair loss effect test

[0091] Three hundred volunteers aged 25-55 years with mild to moderate hair loss were randomly divided into 12 groups of 25 participants each. Each group used an emulsion prepared according to Examples 1-8, Comparative Examples 1-2, Comparative Example 7, and the control group (emulsions prepared according to Comparative Examples 3-6 were unstable and not tested for efficacy). Participants used the test sample twice daily, morning and evening, for three consecutive months. During the test, participants were prohibited from using cosmetics, medications, or devices with hair loss prevention or hair regrowth effects, and from perming or dyeing their hair except for cutting it. The hair loss prevention effect of the samples was evaluated after three months of use, and the results are shown in Table 6.

[0092] Judgment criteria

[0093] Significant effect: Hair loss is significantly improved, and the amount of hair loss is significantly reduced;

[0094] Effective: Hair loss has improved, and the amount of hair loss has decreased, but not significantly;

[0095] Ineffective: There is no improvement in hair loss, and the amount of hair loss has not decreased.

[0096] Effectiveness rate = (Number of people with significant effects + Number of people with effective results) / Total number of people × 100%

[0097] Table 6. Statistical Table of Hair Loss Prevention Test Results

[0098] Number of people with obvious effects Valid number of people Invalid number efficiency Application Example 1 2 15 8 68％ Application Example 2 7 15 3 88％ Application Example 3 3 17 5 80％ Application Example 4 3 15 7 72％ Application Example 5 6 15 4 84％ Application Example 6 3 13 9 64％ Application Example 7 0 11 14 44％ Application Example 8 1 10 14 44％ Application Comparative Example 1 0 11 14 44％ Application Comparative Example 2 0 10 15 40％ Application Comparative Example 7 1 12 12 52％ control group 0 2 23 8％

[0099] As can be seen from Table 6, the control group emulsion without the addition of the anti-hair loss composition had a weak anti-hair loss effect, while the anti-hair loss effectiveness of the emulsion system using the composition of the present invention, as applied in Example 2, reached 88%, indicating that the emulsion system of the composition of the present invention has good anti-hair loss performance under certain conditions.

[0100] As can be seen from Application Examples 1-4, within the concentration range of the present invention, with the same amount of dihydrosphingosine added, changing the ratio of coastal pine bark extract and tea extract can adjust the anti-hair loss efficacy of the composition. When the ratio of coastal pine bark extract to tea extract is 1:(1.1-2.2), a better anti-hair loss effect can be achieved while maintaining the stability of the emulsion.

[0101] Application Examples 5-8 demonstrate that the addition of dihydrosphingosine contributes to enhancing the anti-hair loss effect of the composition. When the amount of dihydrosphingosine added is very low (0.01%, 0.1%), its effect on the anti-hair loss effect is not significant; however, when the amount added reaches 1%, the anti-hair loss effect of the sample emulsion is significantly enhanced. When the amount of dihydrosphingosine reaches 3%, the corresponding sample's anti-hair loss efficiency reaches 84%. Further increasing the amount of dihydrosphingosine (5%) increases the anti-hair loss efficiency to 88%. Therefore, considering the anti-hair loss effect of the composition, the preferred amount of dihydrosphingosine added in this invention is 3-5%.

[0102] As can be seen from Comparative Examples 1-2, the anti-hair loss effect of the combination of two active ingredients in the composition of the present invention is significantly weaker than that of the combination of three active ingredients, indicating that the three active ingredients in the composition of the present invention have a good synergistic effect.

[0103] Comparing Comparative Example 7 and Application Example 1, it can be found that, under the same conditions, the sample containing pine bark extract showed significantly better anti-hair loss effects than the sample containing grape seed extract, even though both are plant extracts containing proanthocyanidins. This may be because hair loss is a complex issue influenced by multiple factors such as hormones, oxidative damage, inflammatory responses, and the scalp microbial environment. Addressing hair loss by focusing on only one factor often fails to achieve the desired anti-hair loss effect. While both grape seed extract and pine bark extract are rich in proanthocyanidins and have some effect on improving hair loss caused by oxidative stress and scalp inflammation, pine bark extract not only contains abundant proanthocyanidins but also contains other bioactive components such as flavonoids, polysaccharides, caffeic acid, ferulic acid, gallic acid, and vanillic acid. These components can regulate microcirculation, inhibit sebum secretion, and also have a beneficial effect on promoting hair regeneration. Therefore, the synergistic effect of adding pine bark extract, tea extract, and dihydrosphingosine in this example is more significant.

[0104] (3) Anti-stimulation test

[0105] The testing method was based on the "Cosmetic Safety Technical Specifications" (2015 edition).

[0106] General testing method: Select a suitable patch tester and use a closed patch test method. Select at least 30 qualified volunteers to participate in the test. Add the test substance, deionized water, and control sample to the square chamber of the patch tester. Apply the patch tester containing the test substance, deionized water, and control sample to the inner forearm of the subject, and gently press it with the palm of your hand to make it evenly applied to the skin. After 24 hours, remove the patch tester and observe the skin reaction according to the standards in Table 7 at 30 minutes (after the pressure mark disappears), 24 hours, and 48 hours, and record the observation results.

[0107] Following the general method described above, 90 volunteers were selected and divided into three groups (A, B, and C), with 30 volunteers in each group, to conduct patch testing. The test sample for group A was the control sample, which was a 3% sodium lauryl sulfate (SLS) aqueous solution; the test sample for group B was the test substance, which was 0.3% of the hair loss prevention composition of Example 2 added to a 3% sodium lauryl sulfate (SLS) aqueous solution; and the test sample for group C was deionized water.

[0108] The patch tester containing the test substance, deionized water, and control sample was applied to the inner forearm of the subject and removed after 24 hours. Skin reactions were observed at 30 min, 24 h, and 48 h after removing the patch tester according to the standards in Table 7, and the results were recorded. Please refer to Table 8 for the results.

[0109] Table 7. Grading Standards for Skin Reactions in Closed Patch Tests

[0110]

[0111] Table 8. Statistical Table of Human Patch Test Results

[0112]

[0113] As can be seen from Table 8, 3% SLS can easily cause adverse skin reactions. However, the addition of the composition of the present invention can significantly reduce the adverse skin reactions caused by SLS, indicating that the composition prepared by the present invention has good anti-irritant properties. This also suggests another advantage of the composition of the present invention when applied to hair care products.

[0114] In summary, the hair loss prevention composition provided in this application achieves excellent hair loss prevention effects through the combination of coastal pine bark extract, tea extract, and dihydrosphingosine. Among these, the coastal pine bark extract reduces oxidative stress damage to hair follicles through its strong free radical scavenging activity, protecting hair follicles, soothing skin inflammation, regulating microcirculation, and promoting hair regeneration. Tea extract neutralizes free radicals and possesses excellent antioxidant capacity. Furthermore, the tea polyphenols in tea extract can inhibit 5α-reductase activity and promote the proliferation of dermal papilla cells, thereby helping to prevent or treat androgenetic alopecia. Dihydrosphingosine can improve scalp barrier function by inducing differentiation and ceramide production through keratinocyte differentiation. In addition, dihydrosphingosine also inhibits 5α-reductase in sebum and hair follicles and prolongs the hair growth phase, reducing oiliness and preventing hair loss. Furthermore, tea extract can effectively protect the coastal pine bark extract, resulting in good light and heat stability in the prepared composition. Dihydrosphingosine can synergistically enhance the efficacy and mildness of the composition with the coastal pine bark extract and tea extract. These three components can work synergistically. When applied in cosmetics, this composition can not only effectively reduce hair loss but also soothe scalp irritation, strengthen the scalp barrier, and exhibit good stability.

[0115] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A hair loss prevention composition, characterized in that, Its components, by mass percentage, include: 30%-45% of coastal pine bark extract, 50%-65% of tea extract, and 3%-5% of dihydrosphingosine. The ratio of the coastal pine bark extract to the tea extract is 1:(1.1-2.2). The coastal pine bark extract was purchased from Guangzhou Fuwei Biotechnology Co., Ltd., and the tea extract was purchased from Guangzhou Aoxue Chemical Co., Ltd.

2. A method for preparing the hair loss prevention composition as described in claim 1, characterized in that, Mix the components thoroughly.

3. The method for preparing the hair loss prevention composition according to claim 2, characterized in that, The mixing process involves stirring at 200-400 rpm for 4-6 minutes.

4. The use of the hair loss prevention composition as described in claim 1 in the preparation of hair loss prevention cosmetics.

5. The application according to claim 4, characterized in that, The hair loss prevention cosmetics include scalp care products or hair cleansing products. The scalp care products include one or more of scalp serums, conditioners, and hair masks. The hair cleansing products include shampoos.

6. A scalp care product for preventing hair loss, characterized in that, Its components, by weight, include: 0.1-40% oil, 0.1-5% emulsifier, 0.01-2% thickener, 3-10% humectant, 0.1-1% preservative, 0.02-0.1% disodium EDTA, 0.1-10% of the hair loss prevention composition as described in claim 1, and the balance being deionized water.

7. The anti-hair loss scalp care product according to claim 6, characterized in that, The oils include one or more of the following: isopropyl myristate, polydimethylsiloxane, caprylic / capric triglyceride, dioctyl carbonate, hydrogenated polyisobutylene, shea butter, ethylhexyl palmitate, hydrogenated polydecene, isononyl isononanoate, cetyl ethylhexyl, phytosterols, unsaponifiables of olive oil, jojoba seed oil, squalane, rosemary leaf oil, lavender oil, sweet orange oil, sunflower seed oil, and camellia seed oil.

8. The anti-hair loss scalp care product according to claim 6, characterized in that, The emulsifier includes one or more of the following: cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, polyglycerol-10 stearate, glyceryl stearate / PEG-100 stearate, sorbitan sesquioleate, isostearyl alcohol polyether-20, PEG-10 rapeseed sterol, lauryl alcohol polyether-7, polyglycerol-3 methyl glucoside distearate, polyglycerol-3 diisostearate, glyceryl stearate, sucrose stearate, stearyl alcohol polyether-20, cetearyl alcohol polyether-20, polysorbate-60, hydrogenated lecithin, and soybean (Glycine soja) seed extract.

9. The anti-hair loss scalp care product according to claim 6, characterized in that, The thickener includes one or more of the following: carbomer, sodium carbomer, xanthan gum, carrageenan, gellan gum, sclerotinia gum, sodium polyacrylate, hydroxyethyl cellulose, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, acrylate (ester) copolymer, and polyacrylate crosspolymer-6.

10. The anti-hair loss scalp care product according to claim 6, characterized in that, The moisturizer includes one or more of the following: glycerin, diglyceride, glyceryl glucoside, propylene glycol, 1,3-propanediol, butylene glycol, panthenol, sorbitol, betaine, sodium PCA, zinc PCA, hydroxyethyl urea, sodium hyaluronate, and β-glucan.

11. The anti-hair loss scalp care product according to claim 6, characterized in that, The preservatives include one or more of phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, methylparaben, p-hydroxyacetophenone, 1,2-hexanediol, and octyl glycol.

12. A method for preparing an anti-hair loss scalp care product as described in any one of claims 6-11, characterized in that, Mix the components thoroughly.

13. The method for preparing the anti-hair loss scalp care product according to claim 12, characterized in that, Mixing the components evenly includes: The emulsifier and the oil are mixed, heated to 75-85°C, and kept at that temperature for 1-20 minutes until the raw materials are completely dissolved to obtain phase A. The thickener, disodium EDTA and deionized water are mixed and homogenized for 1-5 minutes. Then, the mixture is heated to 75-85°C and kept at that temperature for 5-30 minutes. The mixture is stirred thoroughly until the material is completely dissolved to obtain phase B. After adding phase A to phase B and homogenizing for 3-10 minutes, stir and cool. When the temperature drops to below 45°C, add the preservative, the moisturizer, and the hair loss prevention composition, mix well, and then discharge.

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