A Chinese medicine composition for improving body hypoxia tolerance and improving cardiovascular diseases caused by hypoxia, and its preparation method and application

Abstract

A Chinese medicine composition for improving hypoxia tolerance and improving cardiovascular diseases caused by hypoxia is prepared from the following weight percentage components: 5.0-52.0% American ginseng, 4.0-73.0% salvia miltiorrhiza, 2.0-40.0% northern adenophora and 12.0-80.0% angelica. The four raw materials are combined, decocted twice with water, the decoction is combined, and the relative density is 1.10-1.20 when concentrated to 65°C, ethanol is added, stirred, and the alcohol content is calculated by volume percentage to reach 60-85%, and the filtrate is allowed to stand, filtered, and the filtrate is concentrated under reduced pressure to a relative density of 1.20-1.30 at 65°C, and ethanol is recovered to obtain a concentrated solution, and the concentrated solution is added with water and auxiliary materials to prepare an oral solution, or the concentrated solution is spray-dried, and auxiliary materials are added to prepare capsules, tablets, and granules. The composition has the effects of significantly improving hypoxia tolerance of the body, and can improve the prevention and treatment of cardiovascular diseases such as ischemic hypoxia.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine preparations, and in particular to a Chinese medicine composition for improving hypoxia tolerance of an organism and improving cardiovascular diseases caused by hypoxia, and a preparation method and application thereof. Background Art

[0002] Hypoxia usually refers to the rapid entry into plateau areas. In areas with an altitude of more than 2500m, the air is thin and it is a low-oxygen and low-pressure environment, which causes the body to have a stress response caused by hypoxia, which is mainly manifested as rapid heartbeat, dry mouth, nausea, vomiting, diarrhea, palpitations, shortness of breath, and even general discomfort, coma, shock, etc. Humans or animals will have some stress reactions in an acute hypoxic environment. Excessive stress reactions may cause various functional failures in the body. After the body is damaged to varying degrees, it will cause a series of pathological and physiological changes in the body's functions, metabolism, and structure, and eventually lead to the death of important organs such as the heart and brain due to insufficient energy supply.

[0003] Modern pharmacology has reported many studies on the effects of single Chinese medicines in preventing and treating hypoxia, such as Rhodiola rosea, ginseng, Codonopsis pilosula, Salvia miltiorrhiza, Panax notoginseng, Astragalus membranaceus, etc., which have the effect of improving the body's hypoxia. However, there are few studies on the effective screening of Chinese medicine compositions that have the effect of improving the body's hypoxia tolerance. Compound Codonopsis pilosula tablets are the earliest Chinese medicine compound used in my country to prevent and treat acute mountain sickness. It has been used clinically for more than 30 years to prevent and treat mountain sickness and myocardial ischemia. It is mainly composed of Codonopsis pilosula, Angelica sinensis, Adenophora adenophora, Salvia miltiorrhiza and Tripterygium wilfordii. However, the effect of this Chinese medicine in improving the body's hypoxia tolerance is still weak. Summary of the invention

[0004] The purpose of the present invention is to provide a Chinese medicine composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia, as well as its preparation method and application. The Chinese medicine composition has the effects of significantly improving the body's hypoxia tolerance and improving the prevention and treatment of cardiovascular diseases such as ischemic hypoxia.

[0005] To achieve the above purpose, the present invention provides a Chinese medicine composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia, which is prepared from the following ingredients in weight percentage: American ginseng 5.0-52.0%, salvia miltiorrhiza 4.0-73.0%, northern adenophora 2.0-40.0% and angelica 12.0-80.0%.

[0006] Preferably, the Chinese medicine composition is made of the following components in percentage by weight: 8.1% American ginseng, 22.4% Salvia miltiorrhiza, 39.8% Adenophora adenophora and 29.8% Angelica sinensis.

[0007] Preferably, the traditional Chinese medicine composition is processed into oral liquid, tablets, capsules or granules by adding medicinal or edible excipients.

[0008] Preferably, the excipient is one or more of dextrin, lactose, starch, sucrose, glucose, microcrystalline cellulose, mannose, methyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, and stevioside.

[0009] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia, comprising combining American ginseng, salvia miltiorrhiza, Glehnia littoralis and angelica sinensis, decocting twice with water, combining the decoctions, concentrating to a relative density of 1.10-1.20 at 65°C, adding ethanol, stirring to make the alcohol content reach 60-85% by volume, standing, filtering, decompressing and concentrating the filtrate to a relative density of 1.20-1.30 at 65°C, and recovering ethanol to obtain a concentrated solution, adding water and auxiliary materials to the concentrated solution to prepare an oral solution, or spray drying the concentrated solution, adding auxiliary materials to prepare capsules, tablets and granules.

[0010] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of medicines for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia.

[0011] The raw materials of the invention are scientifically formulated with effective ingredients extracted by scientific methods; the invention optimizes and screens a Chinese medicine composition capable of improving the body's hypoxia tolerance and improving cardiovascular diseases such as myocardial ischemia in normobaric hypoxia, sodium nitrite poisoning experiments, decapitation experiments and isoproterenol-induced myocardial ischemia models, thereby providing a therapeutic scheme for clinical prevention or treatment of the body's hypoxia tolerance and ischemia and hypoxia caused by cardiovascular diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The effect of the Chinese medicine composition of Example 4 on the survival time (ST) of the normal pressure hypoxia tolerance experiment;

[0013] Figure 2 The effect of the Chinese medicine composition of Example 4 on the survival time (ST) of the sodium nitrite poisoning experiment;

[0014] Figure 3 is the effect of each tested drug in Example 5 on the survival time (ST) of the normobaric hypoxia tolerance experiment;

[0015] Figure 4 is the effect of each tested drug on the survival time (ST) of the sodium nitrite poisoning experiment in Example 5;

[0016] Figure 5 is the effect of each tested drug in Example 5 on the survival time (ST) of the global ischemia-hypoxia experiment;

[0017] Figure 6 The effect of each test drug in Example 5 on the activity of GSH-PX in mouse plasma after the normobaric hypoxia tolerance experiment;

[0018] Figure 7 The effect of each test drug in Example 5 on the activity of GSH-PX in mouse plasma after the sodium nitrite poisoning experiment;

[0019] In the above figures: #P<0.05, ##P<0.01. DETAILED DESCRIPTION

[0020] The present invention is further described in detail below in conjunction with specific embodiments.

[0021] The Codonopsis pilosula, Salvia miltiorrhiza, Angelica sinensis and American ginseng used in the animal experiments in this manual are all granules with the same extraction rate. The Golden Fruit Oleander is an extract with a different extraction rate from the others.

[0022] Comparative Example 1

[0023] A Chinese medicinal composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 80% American ginseng, 13.84% salvia miltiorrhiza, 1.54% northern adenophora and 4.61% angelica sinensis.

[0024] Comparative Example 2

[0025] A Chinese medicinal composition for improving hypoxia tolerance and cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 69.56% American ginseng, 24.09% salvia miltiorrhiza, 5.35% northern adenophora and 1.00% angelica sinensis.

[0026] Embodiment 1

[0027] A Chinese medicinal composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 5.44% American ginseng, 7.53% salvia miltiorrhiza, 6.69% northern adenophora and 80.33% angelica sinensis.

[0028] Embodiment 2

[0029] A Chinese medicinal composition for improving hypoxia tolerance and cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 8.07% American ginseng, 22.36% salvia miltiorrhiza, 39.75% northern adenophora and 29.81% angelica sinensis.

[0030] Embodiment 3

[0031] A Chinese medicinal composition for improving hypoxia tolerance and cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 13.13% American ginseng, 72.73% salvia miltiorrhiza, 2.02% adenophora adenophora and 12.12% angelica sinensis.

[0032] Embodiment 4

[0033] A Chinese medicinal composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia is prepared from the following ingredients in percentage by weight: 51.74% American ginseng, 4.47% salvia miltiorrhiza, 31.84% adenophora adenophora and 11.94% angelica sinensis.

[0034] Example 4: Experiment on optimizing the compatibility of traditional Chinese medicines for improving hypoxia tolerance

[0035] 1. Effect of normal pressure hypoxia tolerance test on the survival time of mice

[0036] Experimental animals Kunming male mice, 10 mice per group, were randomly selected and gavaged once a day, 10 mL / kg BW each time, for 35 consecutive days. One hour after the last administration, mice in each group were placed in a 250 mL ground-mouth bottle containing 5 g of sodium lime (1 mouse per bottle), the bottle mouth was sealed with vaseline, and the lid was tightly covered to prevent air leakage. The time was immediately counted, and the survival time (ST) of each mouse was observed and recorded using respiratory arrest as an indicator. The following formula groups were prepared into the required concentrations of the tested Chinese medicines.

[0037] Formula group 1 (Formula 1 of Example): American ginseng (7.34 mg / kg BW), salvia miltiorrhiza (10.17 mg / kg BW), Glehnia littoralis (9.04 mg / kg BW) and Angelica sinensis (108.45 mg / kg BW);

[0038] Formula group 2 (Formula of Example 2): American ginseng (10.90 mg / kg BW), salvia miltiorrhiza (30.19 mg / kg BW), Glehnia littoralis (53.66 mg / kg BW) and Angelica sinensis (40.25 mg / kg BW);

[0039] Formula group 3 (Formula of Example 3): American ginseng (17.73 mg / kg BW), salvia miltiorrhiza (98.18 mg / kg BW), Glehnia littoralis (2.73 mg / kg BW) and Angelica sinensis (16.36 mg / kg BW);

[0040] Formula group 4 (Formula of Example 4): American ginseng (69.85 mg / kg BW), salvia miltiorrhiza (6.04 mg / kg BW), Glehnia littoralis (42.99 mg / kg BW) and Angelica sinensis (16.12 mg / kg BW);

[0041] Formula group 5 (comparative example group 1): American ginseng (108.00 mg / kg BW), salvia miltiorrhiza (18.69 mg / kg BW), Glehnia littoralis (2.08 mg / kg BW) and Angelica sinensis (6.23 mg / kg BW);

[0042] Formula group 6 (comparative example formula group 2): American ginseng (93.91 mg / kgBW), salvia miltiorrhiza (32.51 mg / kgBW), Glehnia littoralis (7.22 mg / kgBW) and Angelica sinensis (1.35 mg / kgBW);

[0043] Model group: 0.9% sodium chloride injection was given by gavage;

[0044] For the propranolol hydrochloride positive control group (2 mg / kg BW), propranolol hydrochloride was dissolved in 0.9% sodium chloride injection and administered intragastrically.

[0045] Figure 1 The effect of the above Chinese medicine composition on the survival time (ST) of the normobaric hypoxia tolerance experiment (compared with the model group; n=7-10), by Figure 1 It can be seen that the survival time of mice in the formula 1 to formula 6 groups and the propranolol hydrochloride group was longer than that of the hypoxia model group to varying degrees. Among them, the survival time of the formula 1 and formula 2 groups was significantly prolonged (P<0.05), indicating that the drug administration can prolong the survival time of mice in a hypoxic environment.

[0046] The survival time of experimental mice in formula 1 to formula 6 was analyzed by DPS software, and the response values ​​of American ginseng, Salvia miltiorrhiza, Glehnia littoralis and Angelica sinensis were respectively subjected to regression analysis, and the regression equation of the survival time Y1 of the normobaric hypoxia-tolerant experimental mice was obtained as follows: Y1=29.36837797+0.07390195055X4, where: Y1 represents the survival time of the normobaric hypoxia-tolerant experimental mice, and X4 is the Angelica sinensis dose. It can be seen from the regression equation that factors X1, X2, and X3 are not included, indicating that American ginseng, Salvia miltiorrhiza, and Glehnia littoralis have almost no effect or little effect on the survival time of the normobaric hypoxia-tolerant experimental mice. Among them, the Angelica sinensis dose is positively correlated with the survival time, and the main effect is obvious, showing that there is a compatibility relationship between the drugs, and it is not a simple additive effect.

[0047] 2. Effect of sodium nitrite poisoning on the survival time of mice

[0048] Experimental animals Kunming male mice, 10 mice in each group, were randomly gavaged once a day, 10mL / kgBW each time, for 35 consecutive days. One hour after the last administration, each group of mice was intraperitoneally injected with sodium nitrite at a dose of 220mg / kg BW (injection volume of 0.1mL / 10g), and the time was immediately counted to record the survival time (ST) of the animals. The following formula groups were prepared into the required concentrations of the tested Chinese medicine.

[0049] Formula group 1 (Formula 1 of Example): American ginseng (7.34 mg / kg BW), salvia miltiorrhiza (10.17 mg / kg BW), Glehnia littoralis (9.04 mg / kg BW) and Angelica sinensis (108.45 mg / kg BW);

[0050] Formula group 2 (Formula of Example 2): American ginseng (10.90 mg / kg BW), salvia miltiorrhiza (30.19 mg / kg BW), Glehnia littoralis (53.66 mg / kg BW) and Angelica sinensis (40.25 mg / kg BW);

[0051] Formula group 3 (Formula of Example 3): American ginseng (17.73 mg / kg BW), salvia miltiorrhiza (98.18 mg / kg BW), Glehnia littoralis (2.73 mg / kg BW) and Angelica sinensis (16.36 mg / kg BW);

[0052] Formula group 4 (Formula of Example 4): American ginseng (69.85 mg / kg BW), salvia miltiorrhiza (6.04 mg / kg BW), Glehnia littoralis (42.99 mg / kg BW) and Angelica sinensis (16.12 mg / kg BW);

[0053] Formula group 5 (comparative example group 1): American ginseng (108.00 mg / kg BW), salvia miltiorrhiza (18.69 mg / kg BW), Glehnia littoralis (2.08 mg / kg BW) and Angelica sinensis (6.23 mg / kg BW);

[0054] Formula group 6 (comparative example formula group 2): American ginseng (93.91 mg / kgBW), salvia miltiorrhiza (32.51 mg / kgBW), Glehnia littoralis (7.22 mg / kgBW) and Angelica sinensis (1.35 mg / kgBW);

[0055] Model group: 0.9% sodium chloride injection was given by gavage;

[0056] For the propranolol hydrochloride positive control group (2 mg / kg BW), propranolol hydrochloride was dissolved in 0.9% sodium chloride injection and administered intragastrically.

[0057] Shengui Tablets Group: Chinese medicine composition with authorized patent 200610130406.7 (Shengui Tablets 135mg / kg), its components are as follows: Codonopsis pilosula (58.1mg / kg), Salvia miltiorrhiza (32.4mg / kg), Glehnia littoralis (21.6mg / kg), Angelica sinensis (16.2mg / kg) and American ginseng (6.8mg / kg);

[0058] Figure 2 The effect of the above Chinese medicine composition on the survival time (ST) of the sodium nitrite poisoning experiment (compared with the model group; n=6-9), by Figure 2It can be seen that compared with the hypoxia model group, the survival time of mice in Formula Group 2 to Formula Group 4 and the propranolol hydrochloride positive control group was significantly prolonged (P<0.05); the survival time of mice in Formula Group 1, Formula Group 6 and Shengui Tablets group tended to be prolonged, but the difference was not statistically significant.

[0059] Using DPS software, regression analysis was performed on the response values ​​of American ginseng, Salvia miltiorrhiza, Glehnia littoralis and Angelica sinensis, and the regression equation of the survival time Y2 of the experimental mice poisoned by sodium nitrite was obtained: Y2 = 61.8484136-0.023871875750X3*X3, where: Y2 represents the survival time of the experimental mice poisoned by sodium nitrite, and X3 is the dose of Glehnia littoralis. From the regression equation, it can be seen that the factors X1, X2, and X4 are not included, indicating that American ginseng, Salvia miltiorrhiza, and Angelica sinensis have almost no effect or little effect on the survival time of the experimental mice poisoned by sodium nitrite. Among them, Glehnia littoralis is negatively correlated with the survival time of experimental animals, showing that there is a compatibility relationship between the drugs, not a simple additive effect.

[0060] The statistical results of the body weight of mice in the above-mentioned test groups at the same time points are shown in Table 1 below. As can be seen from Table 1, there is no statistical difference in body weight among the test groups at the same time points, indicating that the test substance has no effect on the body weight of the experimental animals.

[0061] Table 1 Changes in body weight of mice ( n=10-20)

[0062]

[0063]

[0064] Example 5: Hypoxia Tolerance Confirmation Experiment

[0065] 1. Effect of the survival time of mice in the normobaric hypoxia tolerance test

[0066] Experimental animals Kunming male mice were randomly divided into blank control group, model group, compound codonopsis tablets group and optimized formula group. The compound codonopsis tablets group took 10 times the daily human dose of 0.49g / kgDW. To ensure that the amount of Chinese medicinal materials in the optimized formula group and the compound codonopsis tablets group was consistent, the optimized formula group took 3.4 times the daily human dose of 0.46g / kgDW. Mice were gavaged at the above dose at a volume of 10mL / kgDW, once a day, for 21 consecutive days. One hour after the last administration, except for the blank control group, the mice in each group were placed in a 250mL ground-mouth bottle containing 5g of soda lime (1 per bottle), the bottle mouth was sealed with vaseline, and the cover was tight to prevent air leakage. The time was immediately counted, and respiratory arrest was used as an indicator to observe and record the survival time (ST) of each mouse. The following formula groups were prepared into the required concentrations of the tested Chinese medicine.

[0067] Blank control group: 0.9% sodium chloride injection was given by gavage;

[0068] Model group: 0.9% sodium chloride injection was given by gavage;

[0069] Compound Codonopsis Tablets Group: Its composition is as follows: Codonopsis pilosula 251.4mg / kg), Salvia miltiorrhiza (68.6mg / kg), Angelica sinensis (68.6mg / kg), Glehnia littoralis (45.7mg / kg) and Olea europaea (53.3mg / kg);

[0070] Optimized formula group (formula of Example 2): its composition is as follows: American ginseng (37.1 mg / kg), Salvia miltiorrhiza (102.7 mg / kg), Glehnia littoralis (182.2 mg / kg) and Angelica sinensis (136.7 mg / kg).

[0071] One hour after the last intragastric administration, a normobaric hypoxia tolerance test was performed. The results are as follows Figure 3 (Comparison with model group; n=9-10) showed that compared with the model group, the survival time of mice in the optimized formula group was significantly prolonged (P<0.05), but the survival time of mice in the compound codonopsis tablets group was not significantly prolonged, indicating that the optimized formula can prolong the survival time of mice in an oxygen-deficient environment, and the effect is better than that of the compound codonopsis tablets.

[0072] 2. Effect of sodium nitrite poisoning on the survival time of mice

[0073] Experimental animals Kunming male mice were randomly divided into blank control group, model group, compound codonopsis tablets group (0.49g / kgDW) and optimized formula group (0.46g / kgDW). Mice were intragastrically administered with the above dose at a volume of 10mL / kgDW, once a day for 21 consecutive days. The model group was given an equal amount of normal saline, and the animals were free to drink water and eat during the experiment. One hour after the last administration, each group of animals was intraperitoneally injected with sodium nitrite at a dose of 240mg / kg BW, and the time was immediately counted to record the animal survival time (ST).

[0074] Blank control group: 0.9% sodium chloride injection was given by gavage;

[0075] Model group: 0.9% sodium chloride injection was given by gavage; Compound Codonopsis Tablets group: Its composition is as follows: Codonopsis pilosula 251.4mg / kg), Salvia miltiorrhiza (68.6mg / kg), Angelica sinensis (68.6mg / kg), Glehnia littoralis (45.7mg / kg) and Olea europaea (53.3mg / kg);

[0076] Optimized formula group (formula of Example 2): its composition is as follows: American ginseng (37.1 mg / kg), Salvia miltiorrhiza (102.7 mg / kg), Glehnia littoralis (182.2 mg / kg) and Angelica sinensis (136.7 mg / kg).

[0077] Depend on Figure 4 (Comparison with model group; n=8-10) showed that compared with the hypoxia model group, the survival time of mice in the optimized formula group was significantly prolonged (P<0.05), and the effect was better than that in the compound Codonopsis pilosula group.

[0078] 3. Effect of global ischemia-hypoxia experiment on the survival time of mice

[0079] Experimental animals Kunming male mice were randomly divided into blank control group, normal group, model group, compound codonopsis tablets group (0.49g / kgDW) and optimized formula group (0.46g / kgDW). Mice were intragastrically administered with the above dose of 10ml / kgDW, once a day for 7 consecutive days. The blank control group, normal group and model group were given equal amounts of normal saline, and the animals were free to drink water and eat during the experiment. Half an hour after the last intragastrically administered, except for the blank control group and normal group, the other groups were subcutaneously injected with 20mg / kg of isoproterenol. The blank control group and normal group mice were injected with normal saline. 15min after the injection, except for the blank control group, the mice in the other groups were placed in a sealed container of soda lime, and the death time was recorded. The whole heart ischemia model was established with respiratory arrest as the indicator. The protection rate was calculated according to the formula: (survival time of the drug administration group - survival time of the model group) / (survival time of the normal group - survival time of the model group)×100%.

[0080] Blank control group: 0.9% sodium chloride injection was given by gavage;

[0081] Normal group: 0.9% sodium chloride injection was given by gavage;

[0082] Model group: 0.9% sodium chloride injection was given by gavage;

[0083] Compound Codonopsis Tablets Group: Its composition is as follows: Codonopsis pilosula 251.4mg / kg), Salvia miltiorrhiza (68.6mg / kg), Angelica sinensis (68.6mg / kg), Glehnia littoralis (45.7mg / kg) and Olea europaea (53.3mg / kg);

[0084] Optimized formula group (formula of Example 2): its composition is as follows: American ginseng (37.1 mg / kg), Salvia miltiorrhiza (102.7 mg / kg), Glehnia littoralis (182.2 mg / kg) and Angelica sinensis (136.7 mg / kg).

[0085] Depend on Figure 5 It can be seen that compared with the normal group, the survival time of mice in the model group was significantly shortened, indicating that the mice had shown myocardial hypoxia; compared with the model group, the survival time of mice in the compound Codonopsis tablets group and the optimized formula group tended to be prolonged, but the difference was not statistically significant (p>0.05).

[0086] The GSH-PX activity in the plasma of mice in the normobaric hypoxia tolerance experiment was measured using the kit. The results were as follows: Figure 6 As shown in Figure 2, compared with the model group, the compound codonopsis tablets group and the optimized formula group could significantly increase the GSH-PX activity in mouse plasma (P<0.05); the results of the sodium nitrite poisoning experiment are shown in Figure 2. Figure 7 As shown in the figure, the GSH-PX activity in the plasma of mice in the compound codonopsis tablets group and the optimized formula group was higher than that in the hypoxia model group, and the results were significantly different (P<0.05). Both results indicate that the compound codonopsis tablets and the optimized formula have a strong effect in increasing the activity of GSH-PX in plasma.

[0087] Statistical analysis: SPSS 26.0 statistical software was used to perform one-way analysis of variance. P<0.05 indicated that the difference between the groups was statistically significant; DPS statistical software was used to analyze the compatibility relationship between drugs.

Claims

1. A Chinese medicine composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia, It is characterized in that The invention is prepared from the following ingredients in weight percentage: 5.0-52.0% of American ginseng, 4.0-73.0% of salvia miltiorrhiza, 2.0-40.0% of northern adenophora and 12.0-80.0% of angelica sinensis.

2. A Chinese medicine composition for improving hypoxia tolerance and improving cardiovascular diseases caused by hypoxia according to claim 1, It is characterized in that It is made of the following components in percentage by weight: American ginseng 8.07%, Salvia miltiorrhiza 22.36%, Adenophora adenophora 39.75% and Angelica sinensis 29.81%.

3. A Chinese medicine composition for improving hypoxia tolerance and improving cardiovascular diseases caused by hypoxia according to claim 1 or 2, It is characterized in that The traditional Chinese medicine composition is processed into oral liquid, tablet, capsule or granule by adding medicinal or edible excipients.

4. A Chinese medicine composition for improving hypoxia tolerance and improving cardiovascular diseases caused by hypoxia according to claim 3, It is characterized in that The excipient is one or more of dextrin, lactose, starch, sucrose, glucose, microcrystalline cellulose, mannose, methyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose and stevioside.

5. A method for preparing the Chinese medicine composition for improving the body's hypoxia tolerance and improving cardiovascular diseases caused by hypoxia as claimed in claim 1 or 2, It is characterized in that Combine American ginseng, salvia miltiorrhiza, Glehnia littoralis and angelica sinensis, add water and boil twice, combine the decoctions, concentrate to a relative density of 1.10-1.20 at 65°C, add ethanol, stir until the alcohol content reaches 60-85% by volume, let stand, filter, decompress the filtrate to a relative density of 1.20-1.30 at 65°C, and recover the ethanol to obtain a concentrated solution, add water and auxiliary materials to the concentrated solution to prepare an oral solution, or spray-dry the concentrated solution, and add auxiliary materials to prepare capsules, tablets or granules.

6. Use of the Chinese medicine composition as claimed in claim 1 or 2 in the preparation of a medicament for improving cardiovascular diseases caused by hypoxia.

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