A nerve repair composition and its preparation method and application

Abstract

The present invention provides a nerve repair composition and a preparation method and application thereof. The nerve repair composition of the present invention comprises the following components calculated by weight: 0.1-10 parts of curcumin or turmeric powder and 4-460 parts of Acer truncatum seed oil. The present invention can have good anti-aging and nerve repair effects through the synergistic effect between Acer truncatum seed oil and curcumin, and the effect is mild and non-irritating to the human body. The composition of the present invention has significant effects in preventing and treating neurodegenerative diseases, especially depression and improving memory.

Description

Technical Field

[0001] The present invention relates to the technical field of medicine and health care products, in particular to a nerve repair composition and a preparation method and application thereof. Background Art

[0002] Modern society has a fast pace of life, fierce competition, high pressure, and various bad living habits. The brain and nervous system are in a sub-healthy state of tension and fatigue for a long time. The human nervous system is easily damaged, which accelerates matrix aging and makes neurodegenerative diseases more likely to occur. Neurodegenerative diseases are a class of diseases characterized by loss of neuronal function or death, including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. The occurrence of these diseases is related to genetic, environmental, metabolic and other factors. There is currently no effective treatment, which brings huge burden and pain to patients and their families.

[0003] Acer truncatum seed oil is a vegetable oil extracted from the seeds of Acer truncatum. It is rich in active ingredients such as unsaturated fatty acids, vitamin E and phytosterols, and has antioxidant, anti-inflammatory and lipid-lowering effects. Curcumin is a polyphenol compound extracted from the rhizome of turmeric, which has antioxidant, anti-inflammatory and anti-amyloid protein deposition effects. The combination of Acer truncatum seed oil and curcumin may have a synergistic neuroprotective effect and has potential value in the treatment or prevention of neurodegenerative diseases. Therefore, many antioxidant and anti-aging skin care products and health care products have emerged. However, in order to improve the anti-aging effect, a large amount of artificially synthesized antioxidant additives are usually added to the existing anti-aging skin care products. The addition of these additives will produce certain irritation to the human body and affect the nerve repair effect. Although people are committed to developing pure natural antioxidant products with better antioxidant properties, the antioxidant and anti-aging properties of existing products still need to be improved.

[0004] Therefore, it is necessary to provide a new health product with better anti-aging and nerve repair effects. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of existing anti-aging (especially prevention of neurodegenerative diseases) products that they are highly irritating and need to further improve their anti-aging performance, and to provide a new health product composition with better anti-aging and nerve repair effects. The present invention can have good anti-aging and nerve repair effects through the synergistic effect between Acer truncatum seed oil, curcumin and phosphatidylinositol acid, and the effect is mild and non-irritating to the human body.

[0006] Another object of the present invention is to provide a method for preparing the nerve repair composition.

[0007] Another object of the present invention is to provide a use of the nerve repair composition in the preparation of a health product having the efficacy of preventing and treating neurodegenerative diseases.

[0008] To achieve the above object, the present invention adopts the following technical solution:

[0009] A nerve repair composition, comprising the following components calculated in parts by weight:

[0010] 0.1-10 parts of curcumin or turmeric powder, 4-460 parts of Acer truncatum seed oil.

[0011] Curcumin or turmeric powder (containing curcumin) is a polyphenol compound extracted from the rhizome of turmeric, which has the effects of anti-oxidation, anti-inflammatory, and inhibiting amyloid deposition. The curcumin or turmeric powder of the present invention can be an existing commercially available product, or can be extracted or prepared from the rhizome of a ginger plant. The curcumin or turmeric powder can be prepared according to a method comprising the following steps:

[0012] The dried turmeric root powder is added into ethanol and ultrasonically extracted to obtain an ethanol extract, that is, the curcumin; the ethanol extract is vacuum concentrated to obtain a concentrated solution; and the concentrated solution is freeze-dried to obtain the turmeric powder.

[0013] Preferably, the volume fraction of ethanol is 70-95%; the time of ultrasonic extraction is 30-60 minutes; the temperature of ultrasonic extraction is 40-60°C; the pressure of vacuum concentration is 0.05-0.1MPa; the temperature of vacuum concentration is 40-60°C; the temperature of freeze drying is -40 to -20°C.

[0014] The curcumin or turmeric powder contains 2-5wt% of curcuminoids, wherein the content of C1 curcumin is 50-60wt%; the content of C2 demethoxycurcumin is 15-17wt%; and the content of C3 bisdemethoxycurcumin is 0.5-1.5wt%.

[0015] Acer truncatum seed oil is a vegetable oil extracted from the seeds of Acer truncatum. It is rich in active ingredients such as unsaturated fatty acids, vitamin E and plant sterols. It is recognized by scientists around the world as a dual-effect magical substance that can repair and dredge damaged nerve fibers in the brain's nerve pathways and promote the regeneration of nerve cells. Its lack will cause brain diseases such as sequelae of cerebral stroke, Alzheimer's disease, cerebral palsy, brain atrophy, memory loss, insomnia and forgetfulness. It has antioxidant, anti-inflammatory and lipid-lowering effects.

[0016] The Acer truncatum seed oil of the present invention can be an existing commercially available product, or can be extracted or prepared from Acer truncatum seed kernels. In the Acer truncatum seed oil, the relative mass content of linoleic acid is greater than 30%, the relative mass content of arachidonic acid is greater than 12%, and the relative mass content of nervonic acid is greater than 4%.

[0017] Preferably, the Acer truncatum seed oil contains 40-50wt% linoleic acid, 15-20wt% arachidonic acid, and 5-8wt% nervonic acid.

[0018] Preferably, the nerve repair composition comprises the following components calculated in weight percentage:

[0019] 1-5 parts of curcumin or turmeric powder, 45-250 parts of Acer truncatum seed oil.

[0020] Within the above preferred range, the obtained nerve repair composition has better anti-aging and nerve repair effects.

[0021] The inventors of the present invention have found through extensive research that the combined effect of curcumin and Acer truncatum seed oil can significantly improve the antioxidant properties of curcumin and Acer truncatum seed oil, improve the repair of the body's nerves, and effectively prevent and improve neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and ALS.

[0022] The phosphatidylinositol is preferably flavin adenine dinucleotide (FAD). Flavin adenine dinucleotide is a cofactor of flavin coenzyme, which is formed by the conversion of vitamin B2 and participates in various redox reactions in the human body, mainly in the oxidative dehydrogenation of organic matter, such as fatty acids. Phosphatidylinositol can participate in various redox reactions in the human body, and the fatty acids produced by the reaction with the body can regulate the body's metabolism, improve the absorption of vitamins, improve the body's immune level, and improve the antioxidant and nerve repair effects. In addition, the inventors of the present invention have also creatively discovered that phosphatidylinositol, curcumin, and Acer truncatum seed oil can also significantly improve depression patients and memory recovery.

[0023] Therefore, in order to further improve the antioxidant and nerve repair effects, 0.01-2 parts of vitamins may be added to the nerve repair composition, including 0.01-1 parts of vitamin E and 0.01-1 parts of vitamin B6. The vitamin E is natural or synthetic α-tocopherol or its derivatives.

[0024] Preferably, the other excipients are pharmaceutically acceptable excipients, including but not limited to at least one of diluents, preservatives, and suspending agents. The diluent may be one or a combination of polyethylene glycol, propylene glycol, polysorbate, glycerol, peanut oil, corn oil, and soybean oil; the preservative may be one or a combination of benzoic acid, sorbic acid, chlorobutanol, benzyl alcohol, and methyl paraben; the suspending agent may be one or a combination of beeswax, hydrogenated vegetable oil, hydrogenated castor oil, gum arabic, soybean lecithin, cocoa butter, propylene glycol caprylate, caprylic glyceride, carboxymethyl cellulose, hydroxypropyl cellulose, and hydroxyethyl methyl cellulose.

[0025] The present invention also provides a method for preparing the above-mentioned nerve repair composition, comprising the following steps:

[0026] According to the components and dosages, the raw materials of each component are mixed evenly to obtain the nerve repair composition.

[0027] The use of the anti-aging composition in the preparation of health products with the efficacy of preventing and treating neurodegenerative diseases also falls within the scope of protection of the present invention. The neurodegenerative diseases include Alzheimer's disease, Parkinson's disease, ALS, depression, memory impairment, etc.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1) Prolong the survival of ALS (amyotrophic lateral sclerosis, also known as Lou Gehrig's disease) patients, and prevent and delay neurodegenerative diseases. ALS is a fatal neuromuscular disease that causes the gradual degeneration and death of motor neurons, and there is currently no effective treatment. The composition of the present invention can protect motor neurons from oxidative stress and inflammatory damage by providing neuraminic acid and curcumin, delay neuronal function decline, and improve the quality of life of ALS patients.

[0030] 2) Reduce the aggregation of Aβ protein (β-amyloid protein) in AD (Alzheimer's disease) patients and alleviate cognitive impairment. AD is a common senile dementia, mainly manifested by memory loss, loss of language ability, impaired judgment, etc. The main pathological feature of AD is the appearance of senile plaques with Aβ protein deposition in the brain, leading to neuronal death and loss of synaptic function. The composition of the present invention can inhibit the generation and aggregation of Aβ protein, reduce the formation of senile plaques, protect the structure and function of neurons and synapses, and improve the cognitive ability of AD patients by providing neuraminic acid and curcumin.

[0031] 3) Alleviate symptoms of depression and anxiety and improve emotional state. Depression and anxiety are two common mental disorders, mainly manifested as low mood, anxiety, loss of interest, self-denial, etc. Depression and anxiety are related to the imbalance of neurotransmitters in the brain, inflammatory response and oxidative stress. The composition of the present invention can regulate the levels of neurotransmitters such as serotonin, dopamine and norepinephrine in the brain by providing nervonic acid, curcumin and phosphatidylinositol acid, inhibit the release of inflammatory cytokines, scavenge free radicals, improve the neurochemical environment of the brain, and alleviate the symptoms of depression and anxiety. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a graph showing the immobility time in a forced swimming test of mice after being treated with the compositions of Example 1 and various comparative examples in an experiment on improving depression with the composition.

[0033] Figure 2 These are the test results of oxidative stress levels in mice after being treated with the compositions of Example 1 and various comparative examples in an experiment on improving depression using the compositions.

[0034] Figure 3 These are the test results of the oxidative stress level in the hippocampus after being treated with the compositions of Example 1 and various comparative examples in an experiment on improving depression using the compositions.

[0035] Figure 4 These are the test results of the number of errors made by mice in stepping down from the platform after being treated with the compositions of Example 1 and various comparative examples in the memory improvement experiment.

[0036] Figure 5 In the memory improvement experiment of the composition, the results are as follows: the time ratio of mice exploring new objects after being treated with the composition of Example 1 and each comparative example.

[0037] Figure 6 The present invention shows the test results of the number of circles induced in rats with 6-OHDA Parkinson's disease after being treated with the compositions of Example 1 (represented as 6-OHDA / ACP in the figure) and some comparative examples (in the figure, 6-OHDA / A represents treatment with the composition of comparative example 5, 6-OHDA / Cu represents treatment with the composition of comparative example 4, and 6-OHDA represents treatment with the normal saline control group) in the experiment of treating Parkinson's disease. DETAILED DESCRIPTION

[0038] For better explanation of the purpose, technical scheme and advantage of the present invention, the present invention will be further described below in conjunction with specific embodiment and accompanying drawing, but embodiment does not limit the present invention in any form.Unless otherwise specified, the reagent, method and equipment adopted in the present invention are conventional reagents, methods and equipment in the art.Unless otherwise specified, the reagents and materials used in the present invention are commercially available.

[0039] Example 1

[0040] This embodiment provides a nerve repair composition, which is prepared according to the following formula (parts by weight) and the method comprising the following steps:

[0041] The nerve repair composition is obtained by mixing 1 part of turmeric powder, 45.5 parts of Acer truncatum seed oil, 1 part of flavin adenine dinucleotide, 0.5 parts of α-tocopherol, and 0.5 parts of vitamin B6 and stirring evenly.

[0042] The preparation method of turmeric powder comprises the following steps: adding dried turmeric root powder to 95% ethanol by volume, ultrasonically extracting for 60 minutes to obtain an ethanol extract, vacuum concentrating the ethanol extract at 50°C and 0.1MPa to obtain a concentrated solution; freeze-drying the concentrated solution at -40°C to obtain the turmeric powder;

[0043] The prepared turmeric powder contains 5wt% of curcuminoids, wherein the content of C1 curcumin is 60wt%; the content of C2 demethoxycurcumin is 17wt%; the content of C3 bisdemethoxycurcumin is 1.5wt%, and the balance is other types of curcuminoids.

[0044] Example 2

[0045] This embodiment provides a nerve repair composition, which is prepared according to the following formula (parts by weight) and the method comprising the following steps:

[0046] 5 parts of turmeric powder, 228.75 parts of Acer truncatum seed oil, 3 parts of flavin adenine dinucleotide, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 are mixed and stirred evenly to obtain the nerve repair composition; the preparation method of turmeric powder is the same as that of Example 1.

[0047] Example 3

[0048] This embodiment provides a nerve repair composition, which is prepared according to the following formula (parts by weight) and the method comprising the following steps:

[0049] 10 parts of turmeric powder, 459.5 parts of Acer truncatum seed oil, 5 parts of flavin adenine dinucleotide, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 are mixed and stirred evenly to obtain the nerve repair composition; the preparation method of turmeric powder is the same as that of Example 1.

[0050] Example 4

[0051] This embodiment provides a nerve repair composition, which is prepared according to the following formula (parts by weight) and the method comprising the following steps:

[0052] 0.1 parts of turmeric powder, 4.525 parts of Acer truncatum seed oil, 0.1 parts of flavin adenine dinucleotide, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 are mixed and stirred evenly to obtain the nerve repair composition; the preparation method of turmeric powder is the same as that of Example 1.

[0053] Example 5

[0054] This embodiment provides a nerve repair composition, which is prepared according to the method of Example 1. The difference from Example 1 is that α-tocopherol and vitamin B6 are not added.

[0055] Comparative Example 1

[0056] This comparative example provides a nerve repair composition, which is prepared by adding only turmeric powder according to the following steps:

[0057] The nerve repair composition can be obtained by mixing 1 part of turmeric powder, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 and stirring evenly; the preparation method of turmeric powder is the same as that of Example 1.

[0058] Comparative Example 2

[0059] This comparative example provides a nerve repair composition, which is only added with Acer truncatum seed oil and is prepared according to a method comprising the following steps:

[0060] The nerve repair composition is obtained by mixing and stirring 45.5 parts of Acer truncatum seed oil, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 evenly.

[0061] Comparative Example 3

[0062] This comparative example provides a nerve repair composition, which is only added with flavin adenine dinucleotide and is prepared according to a method comprising the following steps:

[0063] The nerve repair composition can be obtained by mixing and stirring 0.1 parts of flavin adenine dinucleotide, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 evenly.

[0064] Comparative Example 4

[0065] This comparative example provides a nerve repair composition, which is a combination of flavin adenine dinucleotide and turmeric powder compared with Example 1, and is prepared according to a method comprising the following steps:

[0066] The nerve repair composition is obtained by mixing and stirring 1 part of turmeric powder, 1 part of flavin adenine dinucleotide, 0.5 part of α-tocopherol and 0.5 part of vitamin B6 evenly.

[0067] Comparative Example 5

[0068] This comparative example provides a nerve repair composition, which is a combination of flavin adenine dinucleotide and Acer truncatum seed oil compared with Example 1, and is prepared according to a method comprising the following steps:

[0069] The nerve repair composition is obtained by mixing and stirring 45.5 parts of Acer truncatum seed oil, 0.1 parts of flavin adenine dinucleotide, 0.5 parts of α-tocopherol and 0.5 parts of vitamin B6 evenly.

[0070] Application Example 1 - Improvement of Depression by the Composition

[0071] The improvement of depression by the composition was determined by forced mouse swimming test and testing the oxidative stress level of the mouse cerebral cortex. The specific operation is as follows:

[0072] Mice aged 8-9 weeks were selected as test subjects (10 mice were selected for each composition for differential analysis and multiple comparisons. In the differential analysis, * indicates p<0.05, ** indicates p<0.01, and *** indicates p<0.001). The nerve repair composition prepared in the above embodiments and comparative examples was used for gavage treatment of mice, and a dominant control group treated with LPS and a blank control group treated with saline were added. Specifically, on days 1 to 5, mice were gavaged at a dosage of 0.02 mL (based on **) / 10 g at the same time every day. On days 3, 4, and 5, lipopolysaccharides (LPS for short, at a dosage of 1 mg / kg) were also intraperitoneally administered while using the above composition for gavage treatment. The difference between the dominant control group and the above composition treatment was that LPS (at a dosage of 1 mg / kg) was administered on days 3, 4, and 5, while the blank group was only administered with saline on days 1 to 5. On the 6th day, the mice were subjected to behavioral tests (detection of the immobility time of the mice in the forced swimming test, in seconds) and tissue sampling to test the oxidative stress level (active oxygen absorption level, expressed as absorbance). The test results are detailed in Figures 1-2 And Tables 1-2.

[0073] In addition, the present invention also carried out oxidative stress level test with hippocampus as the test object according to the above treatment method for mice. The specific test results are shown in Figure 3 .

[0074] Table 1 Immobility time in the forced swimming test of mice

[0075]

[0076] Note: In the table, ACP represents the nerve repair composition of the present invention, and the effective ingredients are "curcumin + Acer truncatum seed oil + phosphatidylcholine"; LPS+A represents "Acer truncatum seed oil + phosphatidylcholine"; LPS+Cu represents "curcumin + phosphatidylcholine"; WT represents the blank control group treated with physiological saline.

[0077] Table 2 Test results of oxidative stress level in mice

[0078]

[0079] Note: In the table, ACP represents the nerve repair composition of the present invention, and the effective ingredients are "curcumin + Acer truncatum seed oil + phosphatidylcholine"; LPS+A represents "Acer truncatum seed oil + phosphatidylcholine"; LPS+Cu represents "curcumin + phosphatidylcholine"; WT represents the blank control group treated with physiological saline.

[0080] Application Example 2: Impact on Memory Improvement

[0081] Experiment 1: Six-month-old mice were treated with the composition obtained in the above examples and comparative examples, and normal saline (as a blank control group), and treated with 0.01 mL / g / d of the Acer truncatum seed oil in the composition for 3 months. The number of errors in the mice stepping down from the platform was detected by an electric stepping platform. Ten mice were selected for each group of composition treatment for differential analysis. The test results are shown in Table 3 and Figure 4 .

[0082] Table 3 Number of errors in mice stepping down the platform

[0083]

[0084] Experiment 2: Ratio of time mice spent exploring novel objects

[0085] Six-month-old mice were divided into 10 mice per group on average. The mice in different groups were treated with the composition obtained in the above embodiments and comparative examples, and normal saline (as a blank control group) at an amount of 0.01 mL / g / d of Acer truncatum seed oil in the composition for 3 months, and then new object recognition was measured. New object recognition refers to: in the training phase, the mice were exposed to two identical objects (A+A) in the room for 5 minutes to become familiar with them. In the test phase, one of the familiar objects was replaced with another new object (A+B), and the mice were put back in the room to detect these objects for 5 minutes, while recording videos and tracking the mice in real time. Detection is defined as the mouse pointing its nose toward the object, sniffing or touching it with its nose, and recording the distance from the nose to the object as ≤2 cm. The discrimination index (DI) is used to explore each object, and the calculation method is: (time to explore the new object - time to explore the old object) / (time to explore the new object + time to explore the old object) * 100. The test results are shown in Table 4 and Figure 5 .

[0086] Table 4 Test results of the time ratio of mice exploring new objects

[0087]

[0088]

[0089] Application Example 3 - Application in the Treatment of Parkinson's Disease

[0090] After 3 weeks of 6-OHDA injection, rats with Parkinson's disease were obtained. The compositions of the above embodiments and comparative examples were used to treat rats with Parkinson's disease (normal saline was used as the control group), and the amount of Acer truncatum seed oil in the composition was 0.01 mL / g / d. After 2 weeks of treatment, the rats were induced to rotate, and the behavioral changes of the rats were observed in a quiet and spacious environment. See the specific test results for details. Figure 6 .

[0091] From the above results we can see that:

[0092] The nerve repair composition prepared in the above embodiment of the present invention has significant effects in improving memory and treating depression and Parkinson's disease.

[0093] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A nerve repair composition, characterized in that: The nerve repair composition is composed of the following components calculated by weight: 0.1-10 parts of curcumin or turmeric powder, 4-460 parts of Acer truncatum seed oil, 0.1-5 parts of flavin adenine dinucleotide, and 0.01-2 parts of other auxiliary materials; The curcumin contains 2-5wt% of curcuminoids, wherein the content of C1 curcumin in the curcuminoids is 50-60wt%; the content of C2 demethoxycurcumin is 15-17wt%; and the content of C3 bisdemethoxycurcumin is 0.5-1.5wt%; The Acer truncatum seed oil contains 40-50wt% of linoleic acid, 15-20wt% of arachidonic acid and 5-8wt% of nervonic acid.

2. The nerve repair composition according to claim 1, characterized in that The composition includes the following components calculated by weight: 1-5 parts of curcumin or turmeric powder, 45-250 parts of Acer truncatum seed oil.

3. The nerve repair composition according to any one of claims 1 to 2, characterized in that: Also includes 0.01-2 servings of vitamins.

4. The nerve repair composition according to claim 3, characterized in that: The vitamins include 0.01-1 part of vitamin E and 0.01-1 part of vitamin B6; the vitamin E is natural or synthetic α-tocopherol or its derivatives.

5. The nerve repair composition according to claim 1, characterized in that: The other auxiliary materials include one or a combination of diluents, preservatives or suspending agents.

6. The method for preparing the nerve repair composition according to any one of claims 1 to 5, characterized in that: The steps include: According to the components and dosages, the raw materials of each component are mixed evenly to obtain the nerve repair composition.

7. Use of the nerve repair composition according to any one of claims 1 to 5 in the preparation of a drug having the efficacy of preventing and treating neurodegenerative diseases.

8. The use according to claim 7, characterized in that: The neurodegenerative diseases include Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, depression, and memory impairment.

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