A compound pesticide for controlling asparagus stem blight

Abstract

The present invention discloses a compound pesticide for preventing and controlling asparagus stem blight, belonging to the technical field of preventing and controlling asparagus stem blight. The compound pesticide for preventing and controlling asparagus stem blight of the present invention comprises the following raw materials in mass percentages: difenoconazole 25-35%, ergot fermentation broth extract 10-15%, additive 3-4%, and the balance is water. The compound pesticide prepared by the present invention has a good prevention and control effect on asparagus stem blight, can reduce the dosage of chemical pesticides, thereby reducing the generation of drug resistance and reducing the pollution and residue of chemical pesticides.

Description

Technical Field

[0001] The present invention relates to the technical field of preventing and controlling asparagus stem blight, and particularly relates to a compound pesticide for preventing and controlling asparagus stem blight. Background Art

[0002] Asparagus (Asparagus officinalis), also known as asparagus fern, belongs to the genus Asparagus in the family Liliaceae. It has extremely high nutritional value, containing rich vitamins B and A, as well as trace elements such as folic acid, selenium, iron, manganese, and zinc, and all essential amino acids for the human body. The selenium content in asparagus is higher than that of general vegetables, close to that of selenium-rich mushrooms, and even comparable to that of sea fish and sea shrimp. Moreover, it has pharmacological functions such as moistening the lungs, relieving cough, reducing phlegm, and inhibiting tumor growth, and is known as the "king of vegetables".

[0003] Asparagus stem blight is known as the cancer of asparagus. When it is mild, individual plants die sporadically, resulting in a reduction in yield; when it is severe, the entire field is destroyed and the yield is completely lost, causing huge losses to asparagus farmers. The pathogen of asparagus stem blight is Phomopsis asparagi, which is one of the most serious diseases on asparagus and occurs worldwide. This disease spreads rapidly under rainy and windy conditions, and can also be transmitted by rain splashing. At present, the prevention and control method of stem blight mainly relies on chemical control. However, when using a single chemical agent for a long time, the drug resistance of the pathogen gradually increases, resulting in a continuous decrease in the drug efficacy. In addition, the use of a large amount of chemical agents will also cause various problems such as environmental pollution and pesticide residues. Therefore, finding an agent that can effectively prevent and control asparagus stem blight and reducing the use amount of chemical agents has become one of the key problems to be solved in this field. Summary of the Invention

[0004] The purpose of the present invention is to provide a compound pesticide for preventing and controlling asparagus stem blight to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions:

[0006] One of the technical solutions of the present invention: A compound pesticide for preventing and controlling asparagus stem blight, comprising the following raw materials in mass percentages: difenoconazole 25 - 35%, ergot fermentation broth extract 10 - 15%, additive 3 - 4%, and the balance is water.

[0007] Further, the preparation method of the ergot fermentation broth extract includes the following steps:

[0008] (1) Activate the ergot fungus and inoculate it into a seed liquid medium for cultivation to obtain an ergot fungus seed liquid;

[0009] (2) Inoculate the ergot fungus seed liquid into a liquid medium for cultivation to obtain an ergot fermentation broth;

[0010] (3) Acidify the ergot fermentation broth and then filter it. Adsorb the filtrate with a cation resin column and then elute it to obtain an elution fraction. The elution fraction is concentrated and dried to obtain the ergot fermentation broth extract.

[0011] Further, in step (1), the seed liquid medium comprises the following components: 55 - 65 g / L of glucose, 12 - 16 g / L of maltodextrin, 2 - 4 g / L of soybean meal powder, 0.8 - 1.2 g / L of potassium dihydrogen phosphate, and 0.2 - 0.4 g / L of magnesium sulfate. The cultivation is shaking cultivation, the rotation speed of the shaking cultivation is 130 - 150 r / min, the temperature is 23 - 25 °C, and the time is 30 - 35 d.

[0012] Further, in step (2), the liquid medium comprises the following components: 80 - 90 g / L of mannitol, 12 - 16 g / L of maltodextrin, 3 - 5 g / L of soybean meal powder, 4 - 6 g / L of yeast powder, and 1.0 - 1.2 g / L of magnesium sulfate. The rotation speed of the cultivation is 150 - 170 r / min, the temperature is 24 - 26 °C, and the time is 16 - 18 d.

[0013] Further, in step (3), the acidification is specifically: adding hydrochloric acid to the ergot fermentation broth to adjust the pH to 2.2 - 2.7, and standing for 2 - 3 h.

[0014] Further, in step (3), the cation resin column is cation resin column D131; the eluent used for elution is ammonia water with a concentration of 0.3 - 0.4 mol / L.

[0015] The main substance in the ergot fermentation broth extract is ergometrine. Ergometrine can bind to the tubulin in the pathogen (Phomopsis asparagi), cause the tubulin to deform, block the process of tubulin assembling into microtubules, thereby preventing the formation of the spindle, causing cell mitosis to stall and leading to cell apoptosis, and further achieving the effect of killing the pathogen.

[0016] Further, the additive comprises the following components in parts by mass: 6 - 8 parts of surfactant, 4 - 5 parts of synergist, 1 - 2 parts of emulsifier, 0.5 - 0.8 parts of thickener, and 0.2 - 0.3 parts of defoamer.

[0017] Further, the surfactant is castor oil, the synergist is momordica saponin, the emulsifier is Tween 80, the thickener is xanthan gum, and the defoamer is a silicone compound.

[0018] Furthermore, the preparation method of the momordicoside includes the following steps: pulverize balsam pear, add ethanol thereto, and perform heating under reflux for extraction; after filtering the extract, pass it through a resin column (HPD100 macroporous resin column, first wash the column with deionized water at normal temperature, and then elute with ethanol with a concentration of 65 vol.%), concentrate the eluate under reduced pressure, and perform spray drying to obtain the momordicoside.

[0019] Momordicoside can promote the absorption and utilization of drugs by plants, improve the dispersion effect of drugs, and thus enhance the drug efficacy.

[0020] The second technical solution of the present invention: A preparation method of the composite pesticide for preventing and treating asparagus stem blight described above, includes the following steps:

[0021] Put the ergot fermentation broth extract and additives into water, stir until completely dissolved, then add difenoconazole, and stir evenly to obtain the composite pesticide for preventing and treating asparagus stem blight.

[0022] The present invention discloses the following technical effects:

[0023] (1) The composite pesticide prepared by the present invention has a good prevention and treatment effect on asparagus stem blight, can reduce the dosage of chemical pesticides, thereby reducing the generation of drug resistance and reducing the pollution and residue of chemical pesticides.

[0024] (2) The present invention uses natural plant extracts to replace a part of chemical pesticides, while reducing the dosage of chemical pesticides, it also ensures the prevention and treatment effect of asparagus stem blight. Specific Embodiments

[0025] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and implementation manners of the present invention.

[0026] It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention. In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0027] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the said documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0028] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the description of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the description of this invention are obvious to those skilled in the art. The description and examples of this application are merely exemplary.

[0029] Regarding the use of "comprising", "including", "having", "containing", etc. in this text, they are all open-ended terms, meaning including but not limited to.

[0030] All "parts" mentioned in the following examples are "parts by mass".

[0031] The ergot used in the following examples and comparative examples of this invention was obtained commercially.

[0032] The purity of difenoconazole used in the following examples and comparative examples of this invention was 99%, and the purity of kasugamycin was 99%.

[0033] Example 1

[0034] A compound pesticide for controlling asparagus stem blight:

[0035] (1) A compound pesticide for controlling asparagus stem blight, which is composed of the following raw materials in mass percentages: difenoconazole 30%, ergot fermentation broth extract 10%, additive 4%, and water 56%.

[0036] (1) Preparation method of ergot fermentation broth extract:

[0037] A. Under sterile conditions, use an inoculation loop to inoculate ergot from a test tube into a potato slant medium (the diameter of the petri dish is 90 mm), inoculate 3 loops per dish, and after inoculation, culture at about 25 °C for 10 d to obtain activated ergot.

[0038] Preparation of potato slant medium: Cut 300 g of peeled potatoes into pieces, add 1000 mL of water, boil for 0.5 h, filter, then add 20 g of glucose and 20 g of agar to the filtrate, make up the volume to 1000 mL with water and adjust the pH value to 5.2, sterilize by moist heat at 121 °C for 20 min, pour into sterilized petri dishes while it is hot (about 20 mL per dish), let it cool and solidify, and then inoculate with Claviceps purpurea.

[0039] B. Scrape off the activated Claviceps purpurea bacterial lawn, add sterilized water and grind it (add 5 mL of sterilized water to the bacterial lawn per dish) to obtain a bacterial suspension; connect the bacterial suspension to the seed liquid medium with a pipette (the volume ratio of the bacterial suspension to the seed liquid medium is 1:100), and culture it by shaking (the rotation speed of shaking culture is 140 r / min, the temperature is 24 °C, and the time is 32 d) to obtain the Claviceps purpurea seed liquid.

[0040] Preparation of seed liquid medium: Add 60 g of glucose, 12 g of maltodextrin, 4 g of soybean meal powder, 1.0 g of potassium dihydrogen phosphate, and 0.3 g of magnesium sulfate to 500 mL of water, mix and dissolve, make up the volume to 1000 mL, adjust the pH value to 5.2, sterilize by moist heat at 121 °C for 20 min to obtain the seed liquid medium.

[0041] C. Inoculate the Claviceps purpurea seed liquid into the liquid medium (the volume ratio of the Claviceps purpurea seed liquid to the liquid medium is 1:50), and culture it (the rotation speed of culture is 160 r / min, the temperature is 24 °C, and the time is 18 d) to obtain the Claviceps purpurea fermentation broth.

[0042] Preparation of liquid medium: Add 85 g of mannitol, 12 g of maltodextrin, 4 g of soybean meal powder, 4 g of yeast powder, and 1.2 g of magnesium sulfate to 500 mL of water, mix and dissolve, make up the volume to 1000 mL, adjust the pH value to 5.2, sterilize by moist heat at 121 °C for 20 min to obtain the liquid medium.

[0043] D. Add a hydrochloric acid aqueous solution with a concentration of 15 wt.% to the Claviceps purpurea fermentation broth to adjust the pH to 2.5, carry out acidification treatment for 2.5 h, then filter, and then add the filtrate to a cation resin column (cation resin column D131, 500 g) for adsorption. After adsorption, wash with 1 L of deionized water, and then elute with ammonia water with a concentration of 0.4 mol / L to obtain an elution fraction; finally, concentrate and dry the elution fraction (at 50 °C) to obtain the Claviceps purpurea fermentation broth extract.

[0044] (2) The additive is composed of the following components in parts by mass: 6 parts of surfactant, 5 parts of synergist, 1 part of emulsifier, 0.5 part of thickener, and 0.2 part of defoamer.

[0045] The surfactant is castor oil; the synergist is momordica saponin; the emulsifier is Tween 80; the thickener is xanthan gum; the defoamer is a silicone compound.

[0046] Preparation method of momordica saponins: After removing the pulp and pulverizing fresh balsam pears, add an ethanol solution with a concentration of 75 vol.% (the mass / volume ratio of fresh balsam pears to the ethanol solution is 1 g: 5 mL) and carry out heating reflux extraction (the extraction temperature is 70 °C and the time is 3 h). After filtering the extract, pass it through a resin column (HPD100 macroporous resin column (500 g), and the filtrate flow rate is 1.8 BV / h). Then, first wash the column with 1.5 L of deionized water at room temperature, and then elute with ethanol with a concentration of 65 vol.%. The eluate is concentrated under reduced pressure and spray-dried to obtain momordica saponins.

[0047] (II) Preparation of compound pesticide

[0048] Put the ergot fermentation broth extract and additives into water, stir until completely dissolved, then add difenoconazole, and stir evenly to obtain a compound pesticide for controlling asparagus stem blight.

[0049] Example 2

[0050] A compound pesticide for controlling asparagus stem blight:

[0051] (I) The compound pesticide for controlling asparagus stem blight is composed of the following raw materials in mass percentages: difenoconazole 25%, ergot fermentation broth extract 15%, additives 3%, and water 57%.

[0052] (1) Preparation method of ergot fermentation broth extract:

[0053] A. The same as Example 1.

[0054] B. Scrape off the activated ergot mycelium, add sterilized water and grind (add 5 mL of sterilized water to each dish of mycelium) to obtain a mycelium suspension; connect the mycelium suspension to a seed liquid medium with a pipette (the volume ratio of the mycelium suspension to the seed liquid medium is 1:100) and shake culture (the rotation speed of the shake culture is 150 r / min, the temperature is 23 °C, and the time is 30 d) to obtain an ergot seed liquid.

[0055] Preparation of seed liquid medium: Add 55 g of glucose, 16 g of maltodextrin, 3 g of soybean meal powder, 0.8 g of potassium dihydrogen phosphate, and 0.4 g of magnesium sulfate to 500 mL of water, mix and dissolve, then make up the volume to 1000 mL, adjust the pH value to 5.2, and sterilize by moist heat at 121 °C for 20 min to obtain the seed liquid medium.

[0056] C. Inoculate the ergot seed liquid into a liquid medium (the volume ratio of the ergot seed liquid to the liquid medium is 1:50) and culture (the rotation speed of the culture is 150 r / min, the temperature is 25 °C, and the time is 16 d) to obtain an ergot fermentation broth.

[0057] Preparation of liquid medium: Add 80 g of mannitol, 14 g of maltodextrin, 5 g of soybean meal powder, 6 g of yeast powder, and 1.0 g of magnesium sulfate into 500 mL of water. After mixing and dissolving, make up the volume to 1000 mL, adjust the pH value to 5.2, and sterilize by moist heat at 121 °C for 20 min to obtain the liquid medium.

[0058] D. Add an aqueous hydrochloric acid solution with a concentration of 15 wt.% to the ergot fermentation broth to adjust the pH to 2.2. After acidification treatment for 3 h, filter, and then add the filtrate to a cation exchange resin column (cation exchange resin column D131, 500 g) for adsorption. After the adsorption is completed, wash with 1 L of deionized water, and then elute with ammonia water with a concentration of 0.3 mol / L to obtain an elution fraction; finally, concentrate and dry (at 50 °C) the elution fraction to obtain an ergot fermentation broth extract.

[0059] (2) The additive is composed of the following components in parts by mass: 8 parts of surfactant, 4 parts of synergist, 1 part of emulsifier, 0.8 part of thickener, and 0.3 part of defoamer.

[0060] The surfactant is castor oil; the synergist is momordica saponin (the preparation method of momordica saponin is the same as that in Example 1); the emulsifier is Tween 80; the thickener is xanthan gum; the defoamer is a silicone compound.

[0061] (II) Preparation of compound pesticide

[0062] Put the ergot fermentation broth extract and the additive into water, stir until completely dissolved, and then add difenoconazole, and stir evenly to obtain a compound pesticide for controlling asparagus stem blight.

[0063] Example 3

[0064] A compound pesticide for controlling asparagus stem blight:

[0065] (I) The compound pesticide for controlling asparagus stem blight is composed of the following raw materials in mass percentages: 35% of difenoconazole, 12% of ergot fermentation broth extract, 3% of additive, and 50% of water.

[0066] (1) Preparation method of ergot fermentation broth extract:

[0067] A. The same as Example 1.

[0068] B. Scrape off the activated ergot mycelium, add sterilized water and grind (add 5 mL of sterilized water to each petri dish of mycelium) to obtain a mycelium suspension; connect the mycelium suspension to the seed liquid medium with a pipette (the volume ratio of the mycelium suspension to the seed liquid medium is 1:100), and shake culture (the rotation speed of the shake culture is 130 r / min, the temperature is 25 °C, and the time is 35 d) to obtain an ergot seed liquid.

[0069] Preparation of seed liquid medium: Add 65 g of glucose, 14 g of maltodextrin, 2 g of soybean meal powder, 1.2 g of potassium dihydrogen phosphate, and 0.2 g of magnesium sulfate into 500 mL of water. After mixing and dissolving, make up the volume to 1000 mL, adjust the pH value to 5.2, and sterilize by moist heat at 121 °C for 20 min to obtain the seed liquid medium.

[0070] C. Inoculate the ergot seed liquid into the liquid medium (the volume ratio of the ergot seed liquid to the liquid medium is 1:50) and culture (the rotation speed of the culture is 170 r / min, the temperature is 26 °C, and the time is 18 d) to obtain the ergot fermentation broth.

[0071] Preparation of liquid medium: Add 90 g of mannitol, 16 g of maltodextrin, 3 g of soybean meal powder, 5 g of yeast powder, and 1.2 g of magnesium sulfate into 500 mL of water. After mixing and dissolving, make up the volume to 1000 mL, adjust the pH value to 5.2, and sterilize by moist heat at 121 °C for 20 min to obtain the liquid medium.

[0072] D. Add an aqueous hydrochloric acid solution with a concentration of 15 wt.% to the ergot fermentation broth to adjust the pH to 2.7. After acidification treatment for 2 h, filter, and then add the filtrate into a cation exchange resin column (cation exchange resin column D131, 500 g) for adsorption. After the adsorption is completed, wash with 1 L of deionized water, and then elute with ammonia water with a concentration of 0.4 mol / L to obtain the eluate fraction; finally, concentrate and dry (at 50 °C) the eluate fraction to obtain the ergot fermentation broth extract.

[0073] (2) The additive is composed of the following components in parts by mass: 7 parts of surfactant, 4 parts of synergist, 2 parts of emulsifier, 0.6 part of thickener, and 0.2 part of defoamer.

[0074] The surfactant is castor oil; the synergist is momordica saponin (the preparation method of momordica saponin is the same as that in Example 1); the emulsifier is Tween 80; the thickener is xanthan gum; the defoamer is a silicone compound.

[0075] (II) Preparation of compound pesticide

[0076] Put the ergot fermentation broth extract and the additive into water, stir until completely dissolved, and then add difenoconazole, and stir evenly to obtain the compound pesticide for controlling asparagus stem blight.

[0077] Comparative Example 1

[0078] Same as Example 1, the only difference is that the ergot fermentation broth extract is replaced with vinblastine with the same mass percentage.

[0079] Comparative Example 2

[0080] Same as Example 1, the only difference is that difenoconazole is replaced with kasugamycin with the same mass percentage.

[0081] Comparative Example 3

[0082] Same as Example 1, except that the preparation of the liquid medium is as follows: 85 g of glucose, 12 g of maltodextrin, 4 g of soybean meal powder, and 1.2 g of magnesium sulfate are added to 500 mL of water. After mixing and dissolving, the volume is fixed to 1000 mL, the pH value is adjusted to 5.2, and it is sterilized by moist heat at 121 °C for 20 min to obtain the liquid medium.

[0083] Comparative Example 4

[0084] Same as Example 1, except that the synergist (momordica saponin) in the additive is replaced with laurocapram in an equal mass fraction.

[0085] Comparative Example 5

[0086] Same as Example 1, except that the synergist (momordica saponin) in the additive is replaced with benzyl silicone oil 250 in an equal mass fraction.

[0087] Comparative Example 6

[0088] Same as Example 1, except that difenoconazole is replaced with ergosterol fermentation broth extract in an equal mass percentage.

[0089] Comparative Example 7

[0090] Same as Example 1, except that ergosterol fermentation broth extract is replaced with difenoconazole in an equal mass percentage.

[0091] Effect Example 1

[0092] Before the onset of asparagus stem blight or when the disease occurs sporadically, the compound pesticide prepared in the examples and comparative examples of the present invention (the dosage of the active ingredient is 10 g / acre) is sprayed on the whole plant. The main part of the stem is sprayed with the medicine, and the medicine is sprayed with water. The water consumption is 60 L / acre. The second spraying is carried out after 7 d, and clear water is used as the control group. The disease occurrence situation is recorded 14 d after the last pesticide spraying. Five points are randomly selected for sampling investigation in each plot, and the disease occurrence situation of 20 plants is investigated at each point, and the disease index and control effect are calculated.

[0093] The test field is randomly arranged in a randomized block design. The plot area of each treatment (example and comparative example) is 30 m 2 , and each treatment is repeated 4 times.

[0094] Table 1 Disease grading standard

[0095]

[0096] Disease index = ∑(number of diseased plants at a certain disease level × value of that disease level) / (total number of inspected plants × value of the highest disease level) × 100%;

[0097] Control effect = (disease index in the control area - disease index in the treatment area) / disease index in the control area × 100%.

[0098] Table 2 Disease occurrence

[0099] Grouping Active ingredient Disease index (%) Control effect (%) Example 1 Difenoconazole, ergot fermentation broth extract 12.3 80.2 Example 2 Difenoconazole, ergot fermentation broth extract 12.6 79.7 Example 3 Difenoconazole, ergot fermentation broth extract 12.4 80.1 Control Example 1 Difenoconazole, vinblastine 16.7 73.2 Control Example 2 Kasugamycin, ergot fermentation broth extract 18.5 70.2 Control Example 3 Difenoconazole, ergot fermentation broth extract 12.9 79.3 Control Example 4 Difenoconazole, ergot fermentation broth extract 13.2 78.8 Control Example 5 Difenoconazole, ergot fermentation broth extract 13.5 78.3 Control Example 6 Ergot fermentation broth extract 23.4 62.4 Control Example 7 Difenoconazole 11.8 81.0 Control group -- 62.2 --

[0100] As can be seen from Table 1, the compound pesticide prepared by the present invention has a good control effect on asparagus stem blight, can reduce the dosage of chemical pesticides, thereby reducing the generation of drug resistance and reducing the pollution and residue of chemical pesticides.

[0101] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A compound pesticide for controlling asparagus stem blight, characterized in that, Raw materials including the following mass percentages: difenoconazole 25 - 35%, ergot fermentation broth extract 10 - 15%, additive 3 - 4%, and the balance being water; The preparation method of the ergot fermentation broth extract includes the following steps: (1) Activate ergot fungi and inoculate them into a seed liquid medium for cultivation to obtain an ergot fungi seed liquid; (2) Inoculate the ergot fungi seed liquid into a liquid medium for cultivation to obtain an ergot fermentation broth; (3) Acidify the ergot fermentation broth and filter it. The filtrate is adsorbed by a cation resin column and then eluted to obtain an elution fraction. The elution fraction is concentrated and dried to obtain the ergot fermentation broth extract; In step (1), the seed liquid medium includes the following components: glucose 55 - 65 g / L, maltodextrin 12 - 16 g / L, soybean meal powder 2 - 4 g / L, potassium dihydrogen phosphate 0.8 - 1.2 g / L, magnesium sulfate 0.2 - 0.4 g / L; the cultivation is shaking cultivation, the rotation speed of the shaking cultivation is 130 - 150 r / min, the temperature is 23 - 25 °C, and the time is 30 - 35 d; In step (2), the liquid medium includes the following components: mannitol 80 - 90 g / L, maltodextrin 12 - 16 g / L, soybean meal powder 3 - 5 g / L, yeast powder 4 - 6 g / L, magnesium sulfate 1.0 - 1.2 g / L; the rotation speed of the cultivation is 150 - 170 r / min, the temperature is 24 - 26 °C, and the time is 16 - 18 d; In step (3), the acidification specifically is: add hydrochloric acid to the ergot fermentation broth to adjust the pH to 2.2 - 2.7, and let it stand for 2 - 3 h; In step (3), the cation resin column is cation resin column D131; the eluent used for elution is ammonia water with a concentration of 0.3 - 0.4 mol / L; The additive includes the following components in parts by mass: surfactant 6 - 8 parts, synergist 4 - 5 parts, emulsifier 1 - 2 parts, thickener 0.5 - 0.8 parts, and defoamer 0.2 - 0.3 parts; The surfactant is castor oil, the synergist is momordica saponin, the emulsifier is Tween 80, the thickener is xanthan gum, and the defoamer is a silicone compound.

2. The preparation method of the composite pesticide for preventing and controlling asparagus stem blight according to claim 1, characterized in that, It includes the following steps: Put the ergot fermentation broth extract and the additive into water, stir until completely dissolved, then add difenoconazole, and stir evenly to obtain the compound pesticide for preventing and controlling asparagus stem blight.

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