Apparatus and method for preparing autologous thrombin

Abstract

The application discloses a preparation device of autologous thrombin, and belongs to the technical field of biomedical engineering, which comprises an activator cylinder and a conducting pipe, a piston head of a piston of the activator cylinder is arranged in an inner container, the inner container is sleeved with an outer cylinder, and a piston seat of the piston is fixedly connected with the outer cylinder. When the outer cylinder is quantitatively moved upward relative to the inner container, the activator in the inner container is pushed by the piston to be added into a test tube through the conducting pipe, so that the activator can be quantitatively added into the test tube, and the added activator can be guaranteed not to be polluted by the outside. The preparation device of autologous thrombin is used to add the activator into centrifuged whole blood to make the whole blood be mixed uniformly, and after standing and contacting, a gel is formed in the mixture, supernatant is formed in the upper part, and the supernatant is sucked to prepare a thrombin solution. The application has the advantages of convenient operation, simple process, effective prevention of external infection, high activity of prepared autologous thrombin and fast gel formation.

Description

Technical Field

[0001] This invention belongs to the field of biomedical engineering technology, and particularly relates to an apparatus and method for preparing autologous thrombin. Background Technology

[0002] Thrombin, as a procoagulant, plays a crucial role in hemostasis. Using autologous blood to prepare thrombin (ATS) for autologous therapy can not only reduce rejection reactions but also decrease viral infections and other side effects.

[0003] When preparing thrombin using autologous blood collected in sealed test tubes, a separating gel and anticoagulant are added to the tubes beforehand. After centrifugation, the blood in the tubes separates into three layers from top to bottom: plasma, white blood cells, and red blood cells. First, the upper layer of the plasma is extracted using a syringe to prepare platelet-rich plasma (PPP). The PRP tube is gently shaken to allow white blood cells to mix into the remaining plasma layer. Then, the plasma layer is extracted again using a syringe to prepare platelet-rich plasma (PRP). The extracted PPP and PRP are added to two separate test tubes. Calcium salt is added to the tube containing PPP to mix it. The tube is then incubated at 37°C for 10 minutes. A gel forms in the tube, activating platelets and forming autologous thrombin (ATS) within the gel. Finally, the gel is added to the tube containing PRP to further activate the PRP, causing it to release more growth factors and cytokines for autologous therapy.

[0004] The above-mentioned preparation of autologous thrombin has disadvantages such as complex procedures, inconvenient operation, and easy risk of external infection. Summary of the Invention

[0005] In view of the technical problems of complicated procedures, inconvenient operation, and easy external infection in the preparation of thrombin in the prior art, the purpose of this invention is to provide a thrombin preparation device and method that is convenient to operate, simple in procedure, can effectively prevent external infection, and produces thrombin with high activity and fast gelation.

[0006] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0007] The apparatus for preparing autologous thrombin includes: an activator cartridge and a conductive tube;

[0008] The activator cartridge includes an inner liner, a piston, and an outer liner, with a connector provided at the upper end of the inner liner;

[0009] A first valve is provided on the conductive pipe, and the first valve is connected to the connector;

[0010] The piston includes a piston head and a piston connecting rod. The piston head is disposed inside the inner liner. One end of the piston connecting rod is connected to the piston head, and the other end of the piston connecting rod is connected to the outer cylinder. The inner liner and the outer cylinder are sleeved together, and the outer cylinder moves relative to the inner liner.

[0011] Preferably, a hook is provided on the outer periphery of the inner liner, and a locking platform is provided on the lower outer periphery of the inner liner, and the outer cylinder can move relative to the inner liner between the locking platform and the hook.

[0012] Preferably, the outer cylinder includes a sleeve, the sleeve is fitted onto the outer cylinder, and the outer cylinder is threadedly connected to the sleeve.

[0013] Preferably, the outer cylinder has axial graduations on its outer periphery, with a graduation range of 0-10 mm; the sleeve has circumferential graduations on its outer periphery, with a graduation range of 0-10 mm.

[0014] Preferably, a spring is fitted on the piston connecting rod, one end of the spring abuts against the lower end of the inner liner, and the other end of the spring is connected to the outer cylinder.

[0015] Preferably, the first valve is a three-way valve, and the three-way valve is connected to the connector.

[0016] Preferably, a second valve is provided on the conductive pipe, and the second valve is a one-way valve.

[0017] Preferably, the second valve is used to connect a test tube, and a side connector is provided on the body of the test tube, and the second valve is connected to the side connector.

[0018] Preferably, a control system is provided on the outer cylinder, and the control system drives the outer cylinder to move up and down relative to the inner liner.

[0019] Preferably, the outer cylinder is equipped with a control system and a motor, and the motor drives the outer cylinder to move up and down relative to the inner liner.

[0020] The method for preparing autologous thrombin using the autologous thrombin preparation device of the present invention comprises the following steps:

[0021] Step a: Select a vacuum PRP tube, wherein the PRP tube contains a separating adhesive;

[0022] Step b: Draw whole blood using a PRP tube;

[0023] Step c: Centrifuge the whole blood in the PRP tube to separate the whole blood, and plasma will form on the top of the separating gel after separation;

[0024] Step d: Start the autologous thrombin preparation device, so that the outer cylinder moves relative to the inner liner and drives the piston to move upward, so that the activator is added to the PRP tube through the connecting tube, so that the activator mixes with the plasma;

[0025] Step e: After sufficient contact and standing, a gel forms in the mixture, and a supernatant forms on the upper part;

[0026] Step f: Absorb the supernatant to obtain an autologous thrombin solution.

[0027] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0028] This invention provides an apparatus and method for preparing thrombin that is convenient to operate, simple in process, can effectively prevent external infection, and produces thrombin with high activity and fast gelation. The thrombin prepared by this method activates PRP to form a gel in a short time and produces a large gel volume. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of an apparatus for preparing autologous thrombin;

[0030] Figure 2 This is a schematic diagram of the activator cartridge;

[0031] Figure 3 This is an exploded view of the activator cartridge;

[0032] Figure 4 This is a cross-sectional view of the activator cartridge;

[0033] Figure 5 This is an enlarged view of a three-way valve;

[0034] Figure 6 This is a schematic diagram of a one-way valve connecting the guide tube and the test tube;

[0035] Figure 7 This is a flowchart of the preparation process of autologous thrombin. Detailed Implementation

[0036] The present invention will be further described in detail below with reference to specific embodiments, but the implementation of the present invention is not limited thereto.

[0037] The apparatus for preparing autologous thrombin of the present invention is as follows: Figure 1-6As shown, the apparatus for preparing autologous thrombin includes: an activator cylinder 1, a connecting tube 2, valves 3, and a test tube 4. The activator cylinder 1 contains the activator. The connecting tube 2 connects the activator cylinder 1 and the test tube 4. Multiple valves 3 can be installed on the connecting tube 2 to control its opening and closing. These valves 3 include valves 31, 32, 33, and 34. When the activator cylinder 1 is connected to valve 31 and the test tube 4 is connected to valve 32, valve 31 is opened to allow the activator in the activator cylinder 1 to flow into the connecting tube 2; valve 32 is opened to allow the activator in the connecting tube 2 to further flow into the test tube 4.

[0038] Activator cartridge 1, specifically as follows Figure 2-5 As shown, the activator cartridge 1 includes an inner liner 12, a piston 13, and an outer cylinder 14. The inner liner 12 contains the activator. The piston head 131 of the piston 13 is disposed inside the inner liner 12, and the inner liner 12 is embedded in the outer cylinder 14. The piston seat 133 of the piston 13 can be fixedly connected to the outer cylinder 14. When the outer cylinder 14 moves up and down along the inner liner 12, it can drive the piston 13 to move up and down, thereby realizing the up and down movement of the piston head 131 within the inner liner 12. Because the piston head 131 seals the inner liner 12, and the outer cylinder 14 further seals the inner liner 12, the activator in the inner liner 12 is protected from external contamination.

[0039] The inner liner 12 is equipped with a connector 123, which is located on the top of the inner liner 12. The connector 123 can be directly connected to the guide pipe 2, or it can be connected to the guide pipe 2 through the valve 3.

[0040] A hook 121 is provided on the outer periphery of the inner liner 12. The hook protrudes downward and forms a certain angle with the outer periphery of the inner liner 12. Two hooks 121a and 121b can be symmetrically arranged. A locking platform 122 is provided below the hook, which can be specifically set on the lower outer periphery of the inner liner 12. When the outer cylinder 14 moves upward relative to the inner liner 12, the hook can hold the outer cylinder 14, thereby preventing the outer cylinder 14 from moving further upward; when the outer cylinder 14 moves downward, the locking platform 122 locks the upper opening of the outer cylinder 14, thereby preventing the outer cylinder 14 from moving further downward. In order to ensure the quantitative addition of activator, the distance between the outer cylinder 14 rising from the locking platform 122 and the hook 121 is set to the distance that the piston 13 moves up and down relative to the inner liner 12.

[0041] The piston 13 includes a piston connecting rod and piston heads 131 and piston seats 133 located at its two ends. The piston head 131 is located inside the inner liner 12. The piston head 131 not only seals the lower end of the inner liner 12, but also pushes the activator in the inner liner 12 into the conduit 2. The piston seat 133 is fixedly connected to the outer cylinder 14. A sealing ring 132 made of elastic material is provided on the outer periphery of the piston head 131. Specifically, the sealing ring 132 can be embedded in the groove on the outer periphery of the piston head 131. When the piston head 131 moves up and down inside the inner liner 12, it further improves the sealing effect between the piston head 131 and the inner liner 12. A spring 16 is provided on the piston connecting rod of the piston 13. Specifically, the spring 16 can be sleeved on the piston connecting rod of the piston 13. The upper end of the spring 16 abuts against the lower end of the inner liner 12, and the lower end of the spring 16 is connected to the outer cylinder 14. Spring 16 is used to limit the vertical movement range of piston head 131, which can prevent piston head 131 from falling out of inner liner 12 when it moves downward to the lower end of inner liner 12, and can also prevent piston head 131 from being damaged when it moves upward to the top of inner liner 12.

[0042] The inner liner 12 is embedded in the outer cylinder 14, forming a sealed connection. When the outer cylinder 14 moves up and down relative to the inner liner 12, it further drives the piston 13 to move up and down, thereby causing the piston head 131 to move up and down within the inner liner 12. When the outer cylinder 14 moves upward relative to the inner liner 12, it drives the piston 13 to move upward, allowing the piston head 131 to push the activator in the inner liner 12 through the connecting tube 2 into the test tube 4. When the activator in the inner liner 12 is used up or the storage amount is insufficient, when the outer cylinder 14 moves downward relative to the inner liner 12, it drives the piston 13 to move downward, allowing the piston head 131 to move downward to a predetermined position within the inner liner 12, at which point the activator can be added to the inner liner 12. To ensure that a fixed amount of activator is added to the test tube 4 each time, the movement of the piston 13 upward relative to the inner liner 12 can be set to a fixed amount, ensuring that the activator in the inner liner 12 enters the test tube 4 through the connecting tube 2. In this way, not only can a quantitative amount of activator be added into test tube 4, but the sealed flow path of the activator also ensures that the added activator is not contaminated by the outside world.

[0043] The outer cylinder 14 is connected to a control system. The control system includes a control board and a power supply. The control system can also include a motor. The control system is connected to the outer cylinder 14, or the motor is directly connected to the bottom of the outer cylinder 14. The power supply can be a battery, an external power source, or a USB interface, etc. When the power is turned on, the control system drives the outer cylinder 14, or the control system controls the motor to directly drive the outer cylinder 14 to move automatically up and down relative to the inner liner 12, thereby moving the piston 13.

[0044] The autologous thrombin preparation apparatus of the present invention may also be equipped with a mounting plate and a worktable, with the mounting plate perpendicular to the worktable. An activator cylinder 1, a connecting tube 2, and a valve 3 are fixed on the mounting plate, and a test tube loading rack is fixed on the worktable, into which test tubes 4 can be inserted. A power switch is provided on the worktable to control the power supply of the control system.

[0045] Due to individual differences or varying conditions, the number of platelets in the blood may differ. Even with a quantitative addition of activator, some platelets may remain unactivated, affecting the time and size of thrombin gel formation. In such cases, a small amount of activator needs to be added to further activate platelets and produce more thrombin. To control the amount of activator added and avoid waste, the outer cylinder 14 is designed with openings at both the top and bottom. A sleeve 15 can be attached to the outer cylinder 14. The sleeve 15 is fitted onto the outer cylinder 14. Alternatively, the outer cylinder 14 can have an external thread 143, and the sleeve 15 can have an internal thread 153. The external thread 143 connects to the internal thread 153, thus creating a threaded connection between the sleeve 15 and the outer cylinder 14. In this case, the piston seat 133 of the piston 13 can be fixed to the bottom of the sleeve 15, and the lower end of the spring 16 is connected to the sleeve 15. When it is found that the thrombin gel in test tube 4 has a long gel time and is dispersed, the sleeve 15 on the outer tube 14 can be tightened upward to drive the piston 13 to move upward, so that the piston head 131 moves upward slowly in the inner tube 12, thereby adding a small amount of activator to test tube 4.

[0046] To avoid wasting activator, a graduation 144 with a range of 0-10 mm can be provided axially on the outer circumference of the outer cylinder 14. The amount of activator added can be controlled by the graduation change as the sleeve 15 moves upward along the outer cylinder 14. Additionally, a fine graduation 152 with a range of 0-10 mm can be provided circumferentially on the outer circumference of the sleeve 15. Setting the 0 mm position of the sleeve 15 on the outer cylinder 14 as the initial position, when the sleeve 15 is screwed clockwise upward along the outer cylinder 14, the amount of activator added is strictly controlled based on the graduation change of the sleeve 15 on the outer cylinder 14 and the change of the fine graduation on the sleeve 15.

[0047] A boss 141 is provided on the outer periphery of the outer cylinder 14. Two bosses 141a and 141b can be symmetrically provided above or at the same level as the scale 144 on the outer cylinder 14. When the sleeve 15 moves upward on the outer cylinder 14, the boss 141 can prevent the sleeve 15 from moving further upward, which can further control the quantitative addition of a small amount of activator.

[0048] The first valve is valve 31. Valve 31 is enlarged as follows: Figure 5As shown. Valve 31 can be a three-way valve 31. The connecting part 311 of the three-way valve 31 connects three valve ports a, b, and c, with an included angle of 120° between any two adjacent valve ports. The connecting part 311 is equipped with a knob 312. Rotating the knob 312 by 120° each time connects any two of the three valve ports a, b, and c, but these two valve ports are not connected to the remaining valve port. The connector 123 of the inner liner 12 is connected to the guide pipe 2 through the three-way valve 31. Valve port a of the three-way valve 31 can be connected to the connector 123, valve port b can be connected to the guide pipe 21, and valve port c can be connected to the guide pipe 22.

[0049] If the amount of activator does not need to be strictly controlled, the coagulant and calcium salt as components of the activator can be glass bead powder, halloysite nanotubes (HNTs), or other negatively charged substances. The ratio of coagulant to calcium salt can be either one or the other can be directly added to test tube 4 through the conductive tube 21 connected to valve port b and the conductive tube 22 connected to valve port c.

[0050] If strict control of the activator dosage is required, the accelerator and calcium salt should be prepared in a certain ratio. Multiple third valves can be installed on the guide pipe 2, including two-way valves 33 and 34. The dosage of accelerator and calcium salt can be controlled by two-way valve 33, and further controlled by two-way valve 34. Two-way valve 34 acts as a switch; when open, accelerator and calcium salt can be added to the inner liner 12 through the guide pipe 21 connected to valve port b and the connector 123 connected to valve port a; when closed, the addition of activator to the inner liner 12 is stopped.

[0051] Rotate the knob 312 of the three-way valve 31 counterclockwise. When valve port a and valve port b of the three-way valve 31 are connected, when the piston 13 moves downward, the activator prepared by the coagulant and calcium salt is added to the inner liner 12 through the flow connector 123 of the guide tube 21 for storage. Rotate the knob 312 counterclockwise further. At this time, valve port a and valve port b are not connected, but valve port a and valve port c are connected. When the outer cylinder 14 moves upward relative to the inner liner 12, thereby driving the piston 13 to move upward, the piston head 131 pushes the activator in the inner liner 12 upward. The activator can be added to the test tube 4 through the flow connector 123 and the guide tube 22.

[0052] The connecting tube 22 connects the activator cylinder 1 and the test tube 4. A second valve, such as valve 32, can be installed on the connecting tube 22. Valve 32 is a one-way valve, as detailed below. Figure 6 As shown. Valve 32 includes valve port 321, valve ball 322, valve port 323 and filter plug 324. Valve 32 controls the flow of activator through the conduction tube 22 to test tube 4, and does not allow the plasma in test tube 4 to flow back into the conduction tube 22. The filter plug 324 of valve 32 allows a suitable amount of air to enter so that the activator flows smoothly.

[0053] To prevent the blood in test tube 4 from being contaminated by air or contact, test tube 4 can be a vacuum blood collection tube, PRP tube, etc., with the tube body 43 sealed by a cap 42. A side connector 432 can be provided on the side wall of the tube body 43 of test tube 4. The side connector 432 is directly connected to the valve port 323 of the second valve. The side connector 432 and the valve port 323 can also be connected by a replaceable disposable hose, thereby further preventing the connecting tube 22 and valve 32 from being contaminated by aerosols. For accurate blood collection or observation of plasma or gel size, graduations can be provided on the tube body 43 of test tube 4 along the height of the tube body 43.

[0054] The method for preparing the autologous thrombin of the present invention using the above-described autologous thrombin preparation apparatus, as follows: Figure 7 As shown. Figure 7 This is a flowchart of the preparation of thrombin using the above-mentioned autologous thrombin device, which specifically includes the following steps:

[0055] Step a: Select test tube 4. Test tube 4 includes tube body 43 and rubber stopper 42. Test tube 4 is preset to vacuum and can be selected from vacuum blood collection tubes, PRP tubes, etc.

[0056] Pipe body 43 can be made of PRP pipe, and PRP pipe can be pre-filled with separating adhesive and anti-coagulant.

[0057] Step b: Draw whole blood. Use a PRP tube to draw (8-10) ml of whole blood.

[0058] Step c: Centrifuge the whole blood.

[0059] After gently inverting the PRP tube repeatedly, centrifuge it to obtain platelet-rich plasma (PPP) and platelet-poor plasma (PRP). Thrombin can then be prepared using PPP and PRP, or either PPP or PRP alone. The specific steps include:

[0060] Step c1: Centrifuge the whole blood obtained in step c. After centrifugation, the components of the whole blood in the PRP tube from bottom to top are: red blood cells, separating gel, white membrane layer and plasma.

[0061] Step c2: Collect the upper plasma obtained in step c1 to obtain platelet-rich plasma (PPP), and add PPP to the first test tube;

[0062] Step c3: After completing step c2, invert the PRP tube to suspend the white membrane layer in the plasma to obtain platelet-rich plasma (PRP). Collect the platelet-rich plasma and add the PRP to the second test tube.

[0063] Step d: Add activator.

[0064] Insert the PRP tube into the test tube loading rack on the workbench, turn on the power to start the above-mentioned autologous thrombin preparation device; open the three-way valve 31 and valve 32 so that valve port a and valve port c of the three-way valve 31 are connected. When the outer cylinder 14 moves upward relative to the inner liner 12, thereby driving the piston 13 to move upward, the piston head 131 pushes the activator in the inner liner 12 upward. The activator can be added into the PRP tube through the connector 123 and the flow tube 22, and then the activator is mixed with whole blood.

[0065] The activator can be mixed with the centrifuged plasma at a volume ratio of 1:(3-7); alternatively, the activator can be mixed with the extracted PPP or PRP, with the activator occupying 1 / 8 to 1 / 4 of the total volume after mixing.

[0066] The coagulant is glass bead powder, halloysite nanotubes (HNTs), or other negatively charged substances. Calcium salts are added to the coagulant in solid crystalline form or as a solution. The activator can be pre-prepared and stored in activator container 1, or added directly to test tube 4 via connecting tubes 21 and 22 as an adjunct solution. At room temperature, the activator is prepared by mixing (2-10) mg of coagulant per ml of calcium salt solution. Activators can be prepared by adding 10% calcium chloride or 10% calcium gluconate to the coagulant. The calcium salt can be selected from calcium chloride, calcium carbonate, calcium sulfate, calcium gluconate, and mixtures of these calcium salts. Calcium salts can be prepared by mixing any one or more of these, such as mixing 10% calcium chloride and 10% calcium gluconate.

[0067] Step e: Let stand. After standing for 30 minutes to allow sufficient contact, a gel will form in the PRP tube, and a supernatant will form at the top. Squeeze the gel to release thrombin into the supernatant.

[0068] Mixing can be achieved by inverting, shaking, agitating, stirring, or vortexing. Mixing can be done once, repeatedly, or periodically. After mixing, or during mixing, the whole blood, coagulant, and calcium salt are in full contact, and a gel will form after standing for a period of time. For example, the contact and standing time can be 10 to 20 minutes, and the contact and standing temperature can be 20℃-45℃, either at room temperature or higher. Temperatures higher than room temperature are more conducive to accelerating gel formation.

[0069] Step f: Extract the thrombin solution. The supernatant is drawn using a syringe to obtain the autologous thrombin solution.

[0070] To separate the gel and obtain the supernatant, centrifugation can be used to precipitate the gel, blood clots, and any other residual cellular components to the bottom of test tube 4, leaving the supernatant at the top. Squeezing the gel releases more thrombin; the thrombin-containing supernatant can be poured out or extracted with a syringe. For example, after centrifugation at 1500g for 5 minutes, the thrombin-containing supernatant can be aspirated using a syringe or pipette. To maintain the activity of the prepared thrombin, the thrombin solution can be stored at low temperatures, such as at (2-8)°C.

[0071] Thrombin can activate platelets, causing them to release growth factors, cytokines, and chemokines. At the same time, thrombin can convert fibrinogen in PRP into fibrin, thereby further forming a larger gel.

[0072] The thrombin gel can be further prepared in step g: the extracted PRP is added to the thrombin solution obtained in step f, and the thrombin solution and PRP are mixed at a volume ratio of (1-3):10 to further form a larger gel. Here, the PRP can be the PRP collected from whole blood in a PRP tube after centrifugation, or it can be obtained by centrifugation of whole blood in a separate PRP tube.

[0073] The whole blood used in this invention comes from the patient themselves. Whole blood is extracted from the patient's body to prepare autologous thrombin, thus preventing foreign viral infection or rejection reactions. To improve efficacy, the whole blood can be centrifuged to extract PPP and PRP separately. PPP is activated with an activator to prepare thrombin, which is then mixed with PRP. This further activates platelets in the PRP, causing them to release more growth factors, cytokines, and chemokines. Simultaneously, the autologous thrombin converts fibrinogen in the PRP into fibrin, forming a larger fibrin gel. Applying this gel to wounds serves as a tissue sealant and promotes wound healing. Thrombin and PRP can be mixed before use or at the wound site. This method of preparing autologous thrombin eliminates the rejection issues associated with foreign thrombin and the possibility of viral infection.

[0074] The thrombin prepared using coagulants and calcium salts as activators in this invention exhibits high activity. Furthermore, under identical experimental conditions, the thrombin activated with this invention forms a larger gel volume and a shorter activation time for PRP.

[0075] The above are only some embodiments of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An apparatus for preparing autologous thrombin, comprising: Activator cartridge, conductive tube; The activator cartridge includes an inner liner, a piston, and an outer liner, with a connector provided at the upper end of the inner liner; A first valve is provided on the conductive pipe, and the first valve is connected to the connector; Its features are: The piston includes a piston head and a piston connecting rod. The piston head is disposed inside the inner liner. One end of the piston connecting rod is connected to the piston head, and the other end of the piston connecting rod is connected to the outer cylinder. The inner liner and the outer cylinder are sealed together, and the outer cylinder can move relative to the inner liner. The first valve is a three-way valve, and the three-way valve is connected to the connector; The three-way valve's connecting part connects to three valve ports a, b, and c. Valve port a is connected to the connector, and valve ports b and c are respectively connected to a guide tube. The included angle between any two adjacent valve ports is 120°. The connecting part is equipped with a knob. Rotating the knob 120° each time connects any two of the three valve ports, but these two valve ports are not connected to the remaining valve port. A second valve is provided on the conductive tube. The second valve is a one-way valve and is used to connect to the test tube. A side connector is provided on the tube body of the test tube, and the second valve is connected to the side connector. Rotating the knob counterclockwise connects port a and port b of the three-way valve. When the piston moves downward, the activator flows through the connector into the inner liner for storage. Rotating the knob further counterclockwise connects port a and port c. When the outer cylinder moves upward relative to the inner liner, thereby driving the piston upward, the piston head pushes the activator in the inner liner upward. The activator flows through the connector to the connector and then through the second valve into the test tube.

2. The apparatus for preparing autologous thrombin according to claim 1, characterized in that: The inner liner is provided with a hook on its outer periphery and a locking platform on its lower outer periphery. The outer cylinder can move relative to the inner liner between the locking platform and the hook.

3. The apparatus for preparing autologous thrombin according to claim 1, characterized in that: The outer cylinder includes a sleeve, which is fitted onto the outer cylinder, and the outer cylinder is threadedly connected to the sleeve.

4. The apparatus for preparing autologous thrombin according to claim 3, characterized in that: The outer cylinder has axial graduations on its outer periphery, with a graduation range of 0-10 mm; the sleeve has circumferential graduations on its outer periphery, with a graduation range of 0-10 mm.

5. The apparatus for preparing autologous thrombin according to claim 1, characterized in that: A spring is fitted onto the piston connecting rod, with one end of the spring abutting against the lower end of the inner liner and the other end of the spring connected to the outer cylinder.

6. The apparatus for preparing autologous thrombin according to claim 1, characterized in that: A control system is provided on the outer cylinder, and the control system drives the outer cylinder to move up and down relative to the inner liner.

7. The apparatus for preparing autologous thrombin according to claim 1, characterized in that: The outer cylinder is equipped with a control system and a motor, and the motor drives the outer cylinder to move up and down relative to the inner liner.

8. A method for preparing autologous thrombin using the preparation apparatus according to any one of claims 1-7, comprising the following steps: Step a: Select a vacuum PRP tube, which contains a separating adhesive; Step b: Draw whole blood using a PRP tube; Step c: Centrifuge the whole blood in the PRP tube to separate the whole blood, and plasma will form on the top of the separating gel after separation; Step d: Start the autologous thrombin preparation device, so that the outer cylinder moves relative to the inner liner and drives the piston to move upward, so that the activator is added to the PRP tube through the connecting tube, so that the activator mixes with the plasma; Step e: After sufficient contact and standing, a gel forms in the PRP tube, and a supernatant forms at the top; Step f: Absorb the supernatant to obtain an autologous thrombin solution.

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