A method for preparing ginger seeds resistant to stem base rot

Abstract

The application discloses a preparation method of ginger seeds with stem base rot resistance through ginger breeding, and belongs to the field of ginger seed breeding, and comprises the following steps: screening a TD-19 strain, fermentation, mixing, and repeated planting. The TD-19 strain is classified as Fusarium oxysporum, is preserved in the General Microbiological Center of China Microorganism Culture Collection Committee, is located at No. 3, Xili Beichen, Chaoyang District, Beijing, is preserved on August 15, 2023, and has a preservation number of CGMCC No. 40756. The application can avoid infection of ginger with stem base rot, does not affect normal growth of the ginger, reduces the amount of pesticides, reduces the labor cost, and improves the quality of the ginger.

Description

TECHNICAL FIELD

[0001] The present application relates to a method for preparing ginger seeds resistant to stem base rot, belonging to the field of ginger seed breeding. BACKGROUND

[0002] In recent years, with the continuous expansion of the planting area of ginger, the occurrence of ginger diseases also shows an upward trend, especially the stem base rot of ginger, which seriously affects the yield and quality of ginger and has become a major disease in crop production.

[0003] Ginger stem base rot is a typical soil-borne disease and seed-borne disease. Its asexual propagule zoospore can be transmitted in the soil with water flow, and after contacting with the ginger root system or stem base, it invades the tissue to cause disease, and then rapidly expands and spreads upward, causing leaf yellowing, leaf withering and even plant death. After the host is diseased, a large number of sporangia and zoospores can be produced on the surface of the diseased part, which can cause multiple reinfections. At present, the main method for preventing and treating stem base rot is chemical control, but from the perspective of environmental safety and human health, it is urgent to find safe and effective biological control methods and approaches.

[0004] Endophyte-induced plant systemic resistance (ISR) has attracted more and more attention from plant researchers. Endophytes parasitize the plant body and have mutual symbiosis with the host, and the stable growth environment is more conducive to play a role. Through comparative study of the microorganism control effects of multiple Fusarium oxysporum, it is found that the non-pathogenic strain of Fusarium oxysporum is one of the best and most stable biocontrol factors. The non-pathogenic strain of Fusarium oxysporum applies the weak strain cross-protection effect, especially the non-pathogenic strain of the same type as the pathogen, which competes with the pathogen for nutrients and infection sites in the rhizosphere, induces the plant to produce resistance, and prevents or delays the entry and reproduction of the pathogen, thereby achieving the effect of preventing disease.

[0005] However, the current research on the non-pathogenic strain of Fusarium oxysporum is not mature, and the existing non-pathogenic strain of Fusarium oxysporum will affect the normal growth of the plant and reduce the quality of ginger when used. SUMMARY

[0006] In view of the defects in the prior art, the present application provides a ginger seed preparation method for breeding ginger resistant to stem base rot, which can avoid ginger infection with stem base rot, does not affect the normal growth of ginger, reduces the amount of pesticide, reduces labor costs, and improves the quality of ginger.

[0007] To solve the above technical problems, the technical scheme adopted by the present application is as follows:

[0008] A ginger seed preparation method for breeding ginger resistant to stem base rot, comprising the steps of screening a TD-19 strain, fermentation, mixing, and repeated planting.

[0009] The screening TD-19 strain step is that in a planting area where ginger stem base rot is serious, a ginger block with a light disease symptom is collected, washed with tap water, naturally dried, soaked and washed, a tissue block of the diseased ginger block is cut at a disease-healthy boundary, the tissue block is inoculated into PDA culture medium, and incubated at 27-29 DEG C for 4.5-5.5 days, a single strain is obtained through purification, the single strain is identified, and the TD-19 strain is screened out;

[0010] The soaking and washing are that the diseased ginger block is soaked with 75% alcohol for 25-35 seconds, washed with sterile water for 4-6 times, soaked with 0.6% sodium hypochlorite for 4-6 minutes, washed with sterile water for 4-6 times, and then the excess water is absorbed with sterilized filter paper;

[0011] The TD-19 strain is classified and named as Fusarium oxysporum, is preserved in the China General Microbiological Culture Collection Center, 1, Beichen West Road, Haidian District, Beijing, and the preservation date is August 15, 2023, and the preservation number is CGMCC No. 40756;

[0012] The fermentation step is that the TD-19 strain is inoculated into culture medium, incubated at 24-26 DEG C for 7-9 days, and high-temperature sterilized to obtain a TD-fermented product;

[0013] The culture medium is composed of corn flour, brown sugar and water, and the mass fraction of the corn flour is 7-9%, the mass fraction of the brown sugar is 1.5-2.5%, and the mass fraction of the water is 88-92%;

[0014] The mixing step is that the sterilized soil and the TD-19 fermented product are uniformly mixed to obtain mixed soil;

[0015] The mass ratio of the sterilized soil to the TD-19 fermented product is 950-1050:45-55;

[0016] The planting step is that the mixed soil is used for planting ginger seeds, and treated ginger seeds are obtained after harvesting, and the mixed soil is continuously used for repeated planting of the treated ginger seeds;

[0017] The mass of the mixed soil and the number of the ginger seeds are 1000-1100g:9-11.

[0018] Compared with the prior art, the present application has the following advantages:

[0019] (1) The ginger seeds selected and bred according to the present application have the characteristics of resisting stem base rot, compared with ordinary ginger seeds, are slightly infected or not infected with ginger stem base rot during planting, do not affect the normal growth of plants, reduce the use amount of pesticides, reduce the labor cost, and improve the quality of ginger;

[0020] (2) The fermentation product produced by the TD-19 strain screened in the application has the effect of preventing and treating basal stem rot, and it can be found that the ginger cultivated by the TD-19 fermentation product has the characteristics of delaying the onset and even not getting sick. In the first year of prevention and treatment, the prevention and treatment effect after 100 days reached 54.4%, and through years of selection, in the fifth year of prevention and treatment, the prevention and treatment effect after 100 days reached 95.3%;

[0021] (3) The TD-19 fermentation product prepared in the application has different degrees of increase in the activities of peroxidase (POD), phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) related to disease resistance during the cultivation and growth of ginger, especially in the peak period of basal stem rot. In the first year of prevention and treatment, the activity of peroxidase (POD) reached 19.6g -1 FWmin -1 , the activity of phenylalanine ammonia lyase (PAL) reached 9.3 g -1 FWh -1 , and the activity of polyphenol oxidase (PPO) reached 22.4g - 1 FWmin -1 , which shows that TD-19 can cause the body reaction of ginger and promote the body to produce disease resistance substances to respond to pathogenic bacteria. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 The change of peroxidase (POD) activity of ginger leaves cultivated by TD-19 fermentation product in Example 2 and blank control ginger;

[0023] Figure 2 The change of phenylalanine ammonia lyase (PAL) activity of ginger leaves cultivated by TD-19 fermentation product in Example 2 and blank control ginger;

[0024] Figure 3 The change of polyphenol oxidase (PPO) activity of ginger leaves cultivated by TD-19 fermentation product in Example 2 and blank control ginger;

[0025] Figure 4 The change of peroxidase (POD) activity of ginger leaves cultivated by TD-19 fermentation product in Example 3 and blank control ginger;

[0026] Figure 5 The change of phenylalanine ammonia lyase (PAL) activity of ginger leaves cultivated by TD-19 fermentation product in Example 3 and blank control ginger;

[0027] Figure 6The activity change of polyphenol oxidase (PPO) of the ginger leaves of the fermented product culture of TD-19 and the blank control in Example 3. DETAILED DESCRIPTION

[0028] In order to have a clearer understanding of the technical features, objectives and effects of the present application, the specific embodiments of the present application will now be described.

[0029] The determination method of peroxidase (POD) in the example is as follows:

[0030] 1. Enzyme liquid extraction: take 1g of fresh leaves, add 5mL of 0.02mol / L phosphate buffer (pH: 6.0) and a small amount of quartz sand, grind homogenate in ice bath, centrifuge at 12000r / min for 3min, and the supernatant is the crude enzyme extract, which is stored in the cold storage;

[0031] 2. Preparation of reaction mixture: prepare 50mL of 0.1mol / L phosphate (pH6.0) in a beaker, add 28μL of guaiacol, heat and stir on a stirrer until the guaiacol is dissolved, then add 30%H2O219μL and mix evenly, and store in the cold storage;

[0032] 3. Determination: take 2 cuvettes with 1cm light path, add 3mL of reaction mixture and 1mL of phosphate buffer in one cuvette as control, and add 3mL of reaction mixture and 1mL of the above enzyme liquid in the other cuvette, immediately start the stopwatch to record the time, and determine the absorbance at 470nm by spectrophotometer, read the absorbance every 1min, and the change of absorbance per minute is expressed as an enzyme activity unit.

[0033] The method for extracting and determining the activity of phenylalanine ammonia lyase (PAL) is as follows:

[0034] 1. Enzyme liquid extraction: take 1g of fresh leaves, add 10mL of boric acid buffer (0.1mol / L, PH8.8, mercaptoethanol 2mmol / L), grind in ice bath, filter through 4 layers of gauze, centrifuge at 10000r / min for 20min, and take the supernatant to determine the enzyme activity;

[0035] 2. Determination: take 1mL of the above enzyme liquid, add 2mL of 0.01mol / L boric acid buffer, 1mL of 0.02mol / L phenylalanine, and 1mL of distilled water, mix evenly, and place in a 30℃ constant temperature water bath for 60min, then terminate the reaction with 0.2ml of 6mol / L hydrochloric acid, and determine the absorbance at 290nm by spectrophotometer, and the change of OD 290 value is 0.01g -1 FWh -1 as an enzyme activity unit. The control is inactivated enzyme.

[0036] Extraction and activity determination of polyphenol oxidase (PPO):

[0037] 1. Enzyme solution extraction: 1 g of fresh leaves was added to 5 mL of phosphate buffer (0.05 mol / L, pH 6.0), ground in an ice bath, centrifuged at 10,000 r / min for 20 min, and the supernatant was used to determine enzyme activity;

[0038] 2. Determination: 0.2 mL of the above enzyme solution was added to 3.5 mL of phosphate buffer, 1 mL of 0.1 mol / L catechol was mixed, and then placed in a 30°C constant temperature water bath for 30 min. After the reaction was completed, the enzyme reaction was terminated with 10% phosphoric acid, and the absorbance value was determined at 525 nm by spectrophotometry. The change in OD 525 value was 0.01 g -1 FWmin -1 was one unit of enzyme activity, and the control was inactivated enzyme.

[0039] Example 1 Obtaining a non-pathogenic strain of Fusarium oxysporum

[0040] 1. Obtaining of pathogenic bacteria: In the planting area of Anqiu ginger stem base rot disease, collect the diseased ginger blocks with mild symptoms, wash them with tap water, dry naturally, soak them in 75% alcohol for 30 s, wash them with sterile water for 5 times, then soak them in 0.6% sodium hypochlorite for 5 min, wash them with sterile water for 5 times, dry the excess water with sterile filter paper, cut the diseased ginger blocks into 0.5 cm x 0.5 cm tissue blocks at the diseased and healthy interface in a sterile workbench, inoculate the tissue blocks into PDA medium, incubate at 28°C for 5 d, and observe regularly. Use tip mycelium purification method to purify until 12 single strains are obtained, which are numbered as 1-12. Store the purified 12 strains in a 4°C refrigerator.

[0041] 2. Morphological identification of pathogenic bacteria: Use a puncher to inoculate the 5 mm diameter mycelial cake of the purified strain onto a PDA plate, incubate in a 28°C incubator for 7 d, observe the colony morphology and color, measure the colony diameter with a ruler, then wash the mycelium and conidia into a conical flask with sterile water, and observe the morphology of the mycelium and conidia under a microscope;

[0042] The morphology of No. 3 colony is basically the same, the colony color is purple, the back is purple red, the shape is round, the mycelium is fine and fluffy, and the amount of white mycelium is large and fluffy; it is completely different from other pathogenic bacteria in morphological characteristics, and is not the pathogenic bacteria of stem base rot such as Pythium aphanidermatum, and is named as TD-19. Further identification of TD-19 strain was carried out.

[0043] 3. Molecular biological identification of pathogenic bacteria: the TD-19 mycelium on the PDA plate was scraped with a sterilized scalpel, put into a mortar, added with liquid nitrogen for rapid grinding, the DNA of the TD-19 strain was extracted by using a genomic DNA extraction kit, specific primers ITS1 and ITS4 were used for amplification, the r DNA-ITS sequence obtained by sequencing the amplification band was subjected to homologous alignment with the existing ITS sequence of the related strain in Gen Bank, and it was determined that the TD-19 strain was a non-pathogenic strain of Fusarium oxysporum.

[0044] The TD-19 strain screened in the application is classified and named as Fusarium oxysporum, and is preserved in the China General Microbiological Culture Collection Center, located at No. 1, Beichen West Road, Haidian District, Beijing, with a preservation date of August 15, 2023, and a preservation number of CGMCC No. 40756.

[0045] Example 2: First-year selection of ginger seed resistant to stem base rot

[0046] 1. Ginger seed treatment method

[0047] (1) Select a TD-19 single colony, inoculate into 100 mL of culture medium, and culture at 25°C for 7 days, then sterilize at high temperature to obtain TD-19 fermentation product;

[0048] The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of corn flour is 8%, the mass fraction of brown sugar is 2%, and the mass fraction of water is 90%;

[0049] (2) Mix 50g of TD-19 fermentation product with 1kg of sterilized soil, and mix 50g of culture medium in step (1) with 1kg of sterilized soil for blank control, respectively, and inoculate healthy ginger seeds in pots, 10 ginger seeds per pot, 3 repeats for each treatment, and take the average value of the measured data.

[0050] 2. Field test of TD-19 fermentation product for prevention and treatment of ginger stem base rot

[0051] All pots were placed in a planting area where the ginger stem base rot was more serious, watered in time to maintain a certain soil moisture, and the disease symptoms were investigated and recorded after inoculation for 40d, 60d, 80d, 100d and 120d, and the activities of peroxidase (POD), phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) were determined.

[0052] TD-19 fermentation product culture of ginger average after 70d planting, the onset of the disease than the blank control can be delayed 25 days, 100d after the incidence of 45.6%, the control effect reached 54.4%, and TD-19 fermentation product culture of ginger plant stem base appear water stained lesions, although gradually expanded, but in the late degree of rot is lighter, the final number of deaths less. And the blank control in 45d after planting, the onset of the disease, followed by rising incidence, in 100d after all disease, stem base rot and death, the specific control effect as follows:

[0053]

[0054] 3. TD-19 fermentation product culture of ginger and the blank control of ginger leaves peroxidase activity, phenylalanine ammonia lyase (PAL) activity, polyphenol oxidase (PPO) activity were detected, detection time from ginger seedling emergence, all the way in the whole process of stem base rot development, the peroxidase activity curve is obtained Figures 1-3 , wherein, 40d when the ginger seed germination 2-4 leaves, 60d is the initial stage of ginger stem base rot, 80d is the medium term of ginger stem base rot, 100d is the late ginger stem base rot, 120d is the end of ginger stem base rot;

[0055] (1) the change of peroxidase (POD) activity

[0056] From Figure 1 It can be seen that the peroxidase activity of TD-19 fermentation product culture of ginger leaves is always higher than that of the blank control, and the activity fluctuates to be stable at 100d, and in 60-100d, that is, the peak of stem base rot, the activity of peroxidase is always high, and the activity of peroxidase reaches 19.6g -1 FWmin -1 at 80d, which proves that TD-19 fermentation product produces resistance to stem base rot in ginger.

[0057] (2) the change of phenylalanine ammonia lyase (PAL) activity

[0058] From Figure 2 It can be seen that the phenylalanine ammonia lyase (PAL) activity of TD-19 fermentation product culture of ginger leaves is higher than that of the blank control after germination, and reaches the peak at 100d with the growth of ginger, and the activity is 9.3g - 1 FWh -1The TD-19 fermentation product is 1.5 times of the blank control, and the blank control is not changed in the whole process of stem base rot infection. It is shown that after the ginger seeds are treated by the TD-19 fermentation product, the phenylalanine ammonia lyase (PAL) is sensitive and the enzyme activity is high, which further shows that the TD-19 fermentation product can improve the disease resistance of the ginger.

[0059] (3) Activity change of polyphenol oxidase (PPO)

[0060] It can be seen from Figure 3 that the activity of polyphenol oxidase in the leaves of the ginger seeds cultured by the TD-19 fermentation product is obviously higher than that of the blank control after germination, and reaches the peak in the peak period of the stem base rot, and the value is 22.4 g -1 FWh -1 , which is 2.4 times of the blank control, which shows that the resistance of the TD-19 fermentation product to the stem base rot is closely related to the activity of the polyphenol oxidase.

[0061] Example 3: Selection of ginger seeds resistant to stem base rot in the second year

[0062] 1. Treatment method of ginger seeds

[0063] (1) A TD-19 single colony is selected, inoculated into 100 mL of culture medium, and cultured at 25°C for 7 days, and then high-temperature sterilized to obtain a TD-19 fermentation product;

[0064] The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of the corn flour is 8%, the mass fraction of the brown sugar is 2%, and the mass fraction of the water is 90%;

[0065] (2) 50g of the TD-19 fermentation product is uniformly mixed with 1kg of sterilized soil, and 50g of the culture medium in step (1) is uniformly mixed with 1kg of sterilized soil as the blank control, and the ginger seeds selected in the first year are planted in the pots, 10 ginger seeds per pot, 3 repeats are carried out for each treatment, and the average value of the measured data is taken.

[0066] 2. Field test of TD-19 fermentation product for preventing and treating stem base rot of ginger

[0067] All the pots are placed in the planting area where the stem base rot of ginger is serious, water is poured in time to maintain a certain soil humidity, and the disease symptoms are investigated and recorded after 40d, 60d, 80d, 100d and 120d of inoculation, and the related resistance indexes including the activities of peroxidase (POD), phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) are measured.

[0068] TD-19 fermentation product culture of ginger average after 76d planting, the onset of disease, than the blank control can delay the onset of 29 days, 100d after the incidence of 34.5%, the control effect reached 65.5%, the blank control in 47d after planting, the onset of disease, then the incidence continued to rise, in 100d after all disease, stem base appear water spot-like lesions, rot and death, the specific control effect as follows:

[0069]

[0070] 3. TD-19 fermentation product culture of ginger and blank control of ginger leaf peroxidase activity, phenylalanine ammonia lyase (PAL) activity, polyphenol oxidase (PPO) activity were detected, detection time from ginger seedling emergence, all the way in the whole process of stem base rot development, the peroxidase activity curve obtained is shown in Figures 1-3 , wherein, 40d when the ginger seed germination has 2-4 leaves, 60d is the initial stage of ginger stem base rot, 80d is the medium term of ginger stem base rot, 100d is the late stage of ginger stem base rot, 120d is the end of ginger stem base rot;

[0071] (1) the change of peroxidase (POD) activity

[0072] From Figure 4 It can be seen that the activity of peroxidase in ginger leaves is always in a high state, and the activity is the highest at 100d after planting. The disease resistance is stronger than the blank control. The activity data of the second year is higher than that of the first year, which shows that through continuous selection, TD-19 fermentation product continuously infects ginger seeds to produce resistance to stem base rot.

[0073] (2) the change of phenylalanine ammonia lyase (PAL) activity

[0074] From Figure 5 It can be seen that after the second year of screening ginger seeds continue to accept the TD-19 fermentation product infection, the activity of phenylalanine ammonia lyase, the disease resistance index, is slightly improved, but it is still stronger than the activity of the blank control. The activity of phenylalanine ammonia lyase reached 9.8g -1 FWh -1 , which further shows that TD-19 fermentation product can improve the disease resistance of ginger.

[0075] (3) the change of polyphenol oxidase (PPO) activity

[0076] From Figure 6As can be seen from the above table, the activity of polyphenol oxidase in the leaves of the ginger seeds treated with the fermentation product of TD-19 in the second year was obviously higher than that of the blank control after the ginger seeds sprouted in the first year, and the value was always at a high level, and the highest value reached 25.4 g - 1 FWh -1 The highest value in the second year was higher than that in the first year, which indicated that the continuous selection improved the activity of polyphenol oxidase and further improved the disease resistance to stem base rot.

[0077] Example 4: Selection of ginger seeds resistant to stem base rot in the third year

[0078] 1. Ginger seed treatment method

[0079] (1) A single colony of TD-19 was selected and inoculated into 100 mL of culture medium, which was cultured at 25°C for 7 days, and then high-temperature sterilization was performed to obtain a TD-19 fermentation product;

[0080] The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of corn flour is 8%, the mass fraction of brown sugar is 2%, and the mass fraction of water is 90%;

[0081] (2) 1 kg of sterilized soil was mixed with 50 g of the TD-19 fermentation product, and the blank control was mixed with uninfected corn kernels and sterilized soil, and then the second year selected ginger seeds were planted in pots, 10 ginger seeds per pot, and each treatment was repeated 3 times, and the average value of the measured data was taken.

[0082] 2. Test of TD-19 fermentation product for preventing and treating ginger stem base rot

[0083] The pots were placed in a planting area where the ginger stem base rot was serious, water was poured in time to maintain a certain soil moisture, and the disease symptoms were investigated and recorded after 40 days, 60 days, 80 days, 100 days and 120 days of inoculation;

[0084] The ginger seeds treated with the TD-19 fermentation product began to be diseased after 82 days on average, which was 37 days later than the blank control, and the incidence was lower, and the control effect was higher than that in the first and second years, and the specific control effect was as follows:

[0085]

[0086] Example 5: Selection of ginger resistant to stem base rot in the fourth year

[0087] 1. Ginger seed treatment method

[0088] (1) A single colony of TD-19 was selected and inoculated into 100 mL of culture medium, which was cultured at 25°C for 7 days, and then high-temperature sterilization was performed to obtain a TD-19 fermentation product;

[0089] The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of corn flour is 8%, the mass fraction of brown sugar is 2%, and the mass fraction of water is 90%.

[0090] (2) 1 kg of sterilized soil was mixed with 50 g of TD-19 fermentation product, and the blank control was mixed with non-inoculated corn kernels and sterilized soil, and the third-year selected ginger seeds were planted in the pots, 10 ginger seeds per pot, and each treatment was repeated 3 times, and the measured data was averaged.

[0091] 2. TD-19 fermentation product for preventing and treating ginger stem base rot test

[0092] The potted plants were placed in the planting area where the ginger stem base rot was serious, watered in time, and the soil humidity was maintained, and the disease symptoms were investigated and recorded after inoculation for 40 d, 60 d, 80 d, 100 d and 120 d;

[0093] The blank control showed water-stained spots of different sizes at the stem base at 42 d, and the water-stained spots gradually expanded as the plant grew, and the leaves turned yellow, and by 100 d, the blank control plant disease spot surrounded the stem base, causing the stem base tissue to rot and die, the average ginger planted with TD-19 fermentation product delayed the onset of disease by 49 days compared with the blank control, the number of disease-free plants increased, and the control effect reached 84.4%, and the specific control effect was as follows:

[0094]

[0095] Example 6: Fifth-year ginger resistance to stem base rot selection

[0096] 1. Ginger seed treatment method

[0097] (1) A TD-19 single colony was selected and inoculated into 100 mL of culture medium, and cultured at 25°C for 7 days, then high-temperature sterilized to obtain TD-19 fermentation product;

[0098] The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of corn flour is 8%, the mass fraction of brown sugar is 2%, and the mass fraction of water is 90%;

[0099] (2) 1 kg of sterilized soil was mixed with 50 g of TD-19 fermentation product, and the blank control was mixed with non-inoculated corn kernels and sterilized soil, and the fourth-year selected ginger seeds were planted in the pots, 10 ginger seeds per pot, and each treatment was repeated 3 times, and the measured data was averaged.

[0100] 2. TD-19 fermentation product for preventing and treating ginger stem base rot test

[0101] Put the potted plants to the planting area where the stem base rot of ginger is serious, water in time, keep certain soil humidity, investigate and record the disease symptoms after 40d, 60d, 80d, 100d, 120d of inoculation;

[0102] Investigate and record the disease symptoms after 40d, 60d, 80d, 100d, 120d of inoculation of the ginger with the non-pathogenic strain of Fusarium oxysporum, the blank control enters the initial stage of disease at 46d, the ginger cultured with the fermentation product of TD-19 delays the average disease of the blank control for 45 days, the ginger cultured with the fermentation product of TD-19 appears small and few disease spots at the stem base in the initial stage of disease, the control effect reaches 95.3% at 100d, there is still no dead plant at 120d, the ginger is not seriously harmed by the stem base rot at the harvest period, and the specific control effect is as follows:

[0103]

[0104] The results of examples 2-6 prove that the fermentation product of TD-19 is non-pathogenic, does not affect the normal growth of the plant, and can improve the resistance and has strong anti-pathogenic ability.

Claims

1. A method for preparing a ginger seed resistant to basal stem rot by breeding ginger from Zingiber officinale, characterized in that, The application relates to a method for treating ginger seeds. The method comprises the following steps: The taxonomic name of the TD-19 strain is Fusarium oxysporum Fusarium oxysporum , and it is preserved in the China General Microbiological Culture Collection Center, located at No. 3, Beichen West Road, Chaoyang District, Beijing, on August 15, 2023, with the preservation number of CGMCC No. 40756. The fermentation TD-19 strain step is inoculating the TD-19 strain into culture medium, culturing at 24-26 DEG C for 7-9 days, high-temperature sterilization, and obtaining a TD-fermentation product. The culture medium is composed of corn flour, brown sugar and water, wherein the mass fraction of the corn flour is 7-9%, the mass fraction of the brown sugar is 1.5-2.5%, and the mass fraction of the water is 88-92%. The mixing step is mixing the sterilized soil and the TD-19 fermentation product uniformly to obtain mixed soil. The repeated planting step is planting the ginger seeds in the mixed soil, harvesting to obtain treated ginger seeds, and repeatedly planting the treated ginger seeds in the mixed soil.

2. The method for preparing ginger seeds of Zingiber officinale resistant to basal stem rot according to claim 1, characterized in that, The mass ratio of the sterilized soil to the TD-19 fermentation product is 950-1050:45-55.

3. The method for preparing ginger seeds of Zingiber officinale against basal stem rot according to claim 1, wherein, The mass ratio of the mixed soil to the ginger seeds is 1000-1100g:9-11.

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