A method for perspective acquisition of phenotypic characteristics of aquatic animals

Abstract

The present invention mainly relates to a method for perspective acquisition of phenotypic characteristics of aquatic animals, including: wrapping the aquatic animals with porous soft materials having similar particle sizes, similar densities but different softness and hardness, adding water, and the density of the porous soft material being lower than that of the aquatic animals, fixing them so as not to affect image acquisition and not to affect the life of the animals, and acquiring perspective images of the aquatic animals to be measured with an X-ray machine. The animals to be detected can stay in water without stress such as cooling and anesthesia, and the harm to the animals is small; for the X-ray perspective images, the image contrast is high and the edges are clear, which can improve the positioning accuracy and speed of key points; it can collect endoskeletal system data that cannot be collected by 2D phenotypic measurement methods; the aquatic animals can complete data collection quickly after simple fixation, greatly improving the data collection speed; the method can significantly reduce the stress response of aquatic animals, quickly calm them down, and improve the perspective acquisition efficiency, especially suitable for high-throughput acquisition processes.

Description

Technical Field

[0001] The present invention belongs to the technical field of automated digital collection of breeding data, and mainly relates to a method for quickly fixing and perspective collection during the collection of phenotypic characteristic data of aquatic animals, specifically to a method for perspective collection of phenotypic characteristics of aquatic animals. Background Art

[0002] The breeding work of shrimp itself is a systematic project, with various breeding theories and methods, and there are also many applications in production practice. Automation and digitization are the hotspots and trends of current breeding technologies. Through the intelligent collection of breeding data, combined with some equipment, big data flows are formed to enable big data mining and shared applications. At present, the level of intelligence and digitization in the breeding work of shrimp such as Macrobrachium rosenbergii is relatively low. Especially in the data collection stage, it is mainly completed manually. When collecting data, it is usually necessary to gather personnel and transfer scientific and technological personnel from other positions to cooperate. Due to the huge number of sample individuals and the need to collect multiple data for each individual, it takes a long time, and the consistency and accuracy of the data are not high. At the same time, marker technology is required for auxiliary data collection, which will interfere with the growth of individuals and affect synchrony. In short, the collection and digitalization of individual phenotypic trait data in the current family selection process is a very time-consuming and laborious task. Correspondingly, it is also an important focus of digitization and intelligence in current aquatic breeding work. Therefore, the intelligent high-throughput determination system for aquatic biological phenotypes is a technology with important application value.

[0003] Currently, the technical key of the high-throughput determination system for aquatic biological (such as shrimp) phenotypes is to integrate deep learning technology with traditional image processing technology based on computer 2D vision technology. To achieve the automated collection and intelligent analysis of phenotypic data and morphological parameters of Macrobrachium rosenbergii. Currently, when the activity ability of the sample to be measured is weak, the acquisition of 2D images is relatively perfect. However, when the sample to be measured is relatively active, it will seriously affect the acquisition of sample phenotypic data. In current practice, generally, the animal to be measured is specially treated to paralyze or reduce the activity of the animal to be measured to complete the phenotypic determination. Usually, low-temperature treatment or out-of-water stress for a period of time is adopted. However, low temperature and out-of-water are usually a kind of stress to aquatic animals, which will reduce the survival rate of the animal to be measured. In addition, due to the large amount and complexity of background colors in ordinary images, it is easy to interfere with the determination of measurement key points, restricting the accuracy and speed of measurement.

[0004] The record of the foregoing background art knowledge is intended to help those of ordinary skill in the art understand the prior art relatively close to the present invention, and at the same time facilitate the understanding of the inventive concept and technical solution of the present application. It should be clear that, in the absence of clear evidence indicating that the above content was publicly available before the filing date of this patent application, the above background art should not be used to evaluate the novelty of the technical solution of the present application. Summary of the Invention

[0005] To solve at least one of the technical problems mentioned in the above background art, the purpose of the present invention is to provide a method for perspective acquisition of phenotypic characteristics of aquatic animals. By applying a variety of porous soft materials to wrap the aquatic animals and adding water, the aquatic animals can be quickly sedated, improving the perspective acquisition efficiency. When applied to the high-throughput acquisition process, it can significantly save time and cost.

[0006] A method for perspective acquisition of phenotypic characteristics of aquatic animals, comprising:

[0007] Using porous soft materials with similar particle sizes, similar densities but different softness and hardness to wrap the aquatic animals and adding water, and the density of the porous soft materials is lower than the density of the aquatic animals, fixing them so as not to affect image acquisition and not to affect the life of the animals, and using an X-ray machine to acquire perspective views of the aquatic animals to be tested.

[0008] As a preference for the technical solution of the present invention, the similar particle sizes mean that the particle size difference of the porous soft materials is not higher than 10%.

[0009] As a preference for the technical solution of the present invention, the similar densities mean that the density difference of the porous soft materials is not higher than 10%.

[0010] As a preference for the technical solution of the present invention, the density of the porous soft materials is lower than the density of the aquatic animals.

[0011] As a preference for the technical solution of the present invention, the porous soft materials with similar particle sizes, similar densities but different softness and hardness specifically include:

[0012] Sponge particles with a density of 29 g / cm 3 , a 25% compression hardness of 1.9 Kpa; and

[0013] Hard polyurethane particles with a density of 28 - 30 g / cm 3 .

[0014] As a preference for the technical solution of the present invention, the particle size diameter of the porous soft materials is 0.5 - 5 mm.

[0015] As a preference for the technical solution of the present invention, the aquatic animals are temporarily raised in an aquarium system at room temperature for ≥ 7 days before being wrapped with the porous soft materials.

[0016] As a preference for the technical solution of the present invention, the aquatic animals include shrimp.

[0017] As a preference for the technical solution of the present invention, the aquatic animals include Macrobrachium rosenbergii.

[0018] As a preference for the technical solution of the present invention, before using an X-ray machine to acquire perspective views of the animals to be tested, a high-hardness porous soft material is used to cover and fix the aquatic animals to be tested.

[0019] In the past, data collection that needed to be done manually usually required gathering personnel and mobilizing scientific and technological personnel from other positions to collaborate. Moreover, due to the huge number of sample individuals and the need to collect multiple data for each individual, it took a long time, affecting the consistency and accuracy of the data. For auxiliary data collection, marking technology was needed, which would inevitably interfere with the growth of individuals. Based on the above considerations, the present application provides a method for perspective collection of phenotypic characteristics of aquatic animals. After wrapping the aquatic animal with porous soft materials with similar particle sizes, similar densities but different softness and hardness, an X-ray machine is used to collect the perspective view of the aquatic animal to be measured. During the collection process, the aquatic animal does not need to leave the water body, and there is no stress such as cooling and anesthesia, significantly reducing the stress response of the aquatic animal; the X-ray perspective view has high image contrast and clear edges, which can improve the positioning accuracy and positioning speed of key points; endoskeletal system data that cannot be collected by the 2D phenotypic measurement method can be collected; the porous soft material has two hardness values, and the density difference is within 10%. The inventor unexpectedly found that applying porous soft materials with different hardness values and similar density values helps to significantly reduce the stress response of aquatic animals, can quickly calm the aquatic animals, make them quickly quiet, thereby improving the perspective collection efficiency. When applied to the high-throughput collection process, it can significantly save time and cost, complete the high-throughput collection in a short time, and further reduce the stress response of aquatic animals and improve the accuracy of phenotypic characteristic collection.

[0020] The beneficial effects of the present application are as follows:

[0021] (1) The detected animals can stay in water without stress such as cooling and anesthesia, causing little harm to the animals;

[0022] (2) The X-ray perspective view has high image contrast and clear edges, which can improve the positioning accuracy and positioning speed of key points;

[0023] (3) Endoskeletal system data that cannot be collected by the 2D phenotypic measurement method can be collected;

[0024] (4) Aquatic animals can be quickly fixed and the data can be quickly completed, greatly increasing the speed of data collection;

[0025] (5) The method can significantly reduce the stress response of aquatic animals, quickly calm them, and improve the perspective collection efficiency, especially suitable for the high-throughput collection process. Brief Description of the Drawings

[0026] In order to make the above and / or other purposes, features, advantages and examples of the present invention more obvious and easy to understand, the drawings required for use in the specific implementation of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 is a schematic diagram of an embedding container and part of the embedding material used in Example 1;

[0028] Figure 2 This is a schematic diagram of placing a single aquatic animal into an embedding container in Example 1;

[0029] Figure 3 is a front perspective view of a single animal obtained after embedding and fixation in Example 1;

[0030] Figure 4 is a front perspective view obtained after embedding and fixation of multiple animals in Example 2;

[0031] Figure 5 is a side perspective view obtained after embedding and fixation of multiple animals in Example 2;

[0032] Figure 6 It is to obtain a schematic diagram of key positioning points on a plane image of the animal to be inspected;

[0033] Figure 7 The container with holes in Example 3;

[0034] Figure 8 This is a calibration diagram of the side image of Macrobrachium rosenbergii when its posture is straight and stretched. DETAILED DESCRIPTION

[0035] Those skilled in the art can refer to the content of this article and appropriately replace and / or modify the process parameters to achieve the same. However, it should be particularly noted that all similar replacements and / or modifications are obvious to those skilled in the art and are considered to be included in the present invention. The products and preparation methods described in the present invention have been described through preferred examples. It is obvious that relevant personnel can modify or appropriately change and combine the products and preparation methods described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0036] Unless otherwise defined, the technical and scientific terms used herein have the same meanings as commonly understood by those of ordinary skill in the art to which this invention belongs. This invention uses the methods and materials described herein; however, other suitable methods and materials known in the art may also be used. The materials, methods, and examples described herein are illustrative only and not intended to be limiting. All publications, patent applications, patents, provisional applications, database entries, and other references mentioned herein are incorporated herein by reference in their entirety. In case of conflict, the present specification, including definitions, shall prevail.

[0037] Unless otherwise specified, the materials, methods, and examples described herein are exemplary and not restrictive. Although methods and materials similar or equivalent to those described herein may be used in the practice or testing of the present invention, suitable methods and materials are still described herein.

[0038] The present invention is described in detail below.

[0039] Example 1:

[0040] A method for perspective acquisition of phenotypic characteristics of aquatic animals is provided. In this example, Macrobrachium rosenbergii is taken as an example. The whole acquisition system is carried out in an environment of 25°C ± 2°C. The specific steps include:

[0041] S01. Place the Macrobrachium rosenbergii to be tested in an aquarium system at room temperature and hold it for ≥ 7 days.

[0042] S02. Place each Macrobrachium rosenbergii in a container of appropriate size. The container size is 25 cm in length, 6 cm in width, and 6 cm in height, as Figure 1 and 2 shown.

[0043] S03. Put sponge particles with a density of 29 g / cm 3 , a particle size of 1 mm, and a 25% compression hardness of 1.9 Kpa and rigid polyurethane particles with a density of 30 g / cm 3 , a particle size of 1 mm to cover the animal to be tested. Add an appropriate amount of water to ensure that the gill filaments of the animal to be tested are not dry. Cover the surface with a rigid polyurethane board with a density of 30 g / cm 3 and a thickness of 2 mm to complete the fixation of Macrobrachium rosenbergii.

[0044] S04. Send the fixed animal to be tested into an X-ray machine through an automated device or manually to acquire a perspective view of the animal to be tested. The X-ray machine parameters are kvp: 40 mA: 80.00 ms: 16.00 mAs: 1.25, as Figure 3 shown, Figure 3The embedding material can not only fix the measured animal but also not affect imaging. The key imaging points have a strong contrast with the surrounding area, which is conducive to the software to quickly and accurately capture and establish the key points.

[0045] S05. Collect the phenotypic data of the animal to be measured through image analysis software.

[0046] S06. Archive the collected phenotypic data digitally.

[0047] Example 2:

[0048] Provide a method for collecting the phenotypic characteristics of aquatic animals. In this example, the same batch of Macrobrachium rosenbergii as in Example 1 is taken as an example. The whole collection system is carried out in an environment of 25°C ± 2°C. The specific steps are as follows:

[0049] S01. Temporarily raise the Macrobrachium rosenbergii in an aquarium system at room temperature for ≥ 10 days. The Macrobrachium rosenbergii belongs to the same batch of aquatic animals as those in Example 1.

[0050] S02. Place 2 Macrobrachium rosenbergii in the container described in Example 1.

[0051] S03. Put sponge particles with a density of 29 g / cm 3 , a particle size of 1 mm, and a 25% compression hardness of 1.9 Kpa and rigid polyurethane particles with a density of 30 g / cm 3 , a particle size of 1 mm to cover the animal to be measured, add an appropriate amount of water to ensure that the gill filaments of the animal to be measured are not dry, and cover the surface with a rigid polyurethane board with a density of 30 g / cm 3 and a thickness of 2 mm to complete the fixation of the Macrobrachium rosenbergii.

[0052] S04. Send the fixed animal to be measured into the X-ray machine through an automated device or manually to collect the perspective view of the animal to be measured. The parameters of the X-ray machine are kvp: 40 mA: 80.00 ms: 16.00 mAs: 1.25. Its front perspective view is as shown in Figure 4 and the side perspective view is as shown in Figure 5 .

[0053] S05. Cut each shrimp out of the image using image processing software.

[0054] S06. Collect the phenotypic data of the animal to be measured through image analysis software. The schematic diagram of the positioning key points on the plane picture of the animal to be inspected is as shown in Figure 6 .

[0055] S07. Archive the collected phenotypic data digitally.

[0056] Comparative Example 1:

[0057] A method for collecting perspective phenotypic characteristics of aquatic animals is provided. In this embodiment, Macrobrachium rosenbergii of the same batch as in Example 1 is taken as an example. The entire collection system is carried out in an environment of 25°C ± 2°C. The specific steps include:

[0058] S01. Place the Macrobrachium rosenbergii to be tested in an aquarium system at room temperature and acclimate for ≥ 7 days.

[0059] S02. Place each Macrobrachium rosenbergii into the container described in Example 1.

[0060] S03. Wrap and cover the animal to be tested with sponge particles having the same density as in Example 1, i.e., 29 g / cm 3 , a particle size of 1 mm, and a 25% compression hardness of 1.9 Kpa. Add an appropriate amount of water to ensure that the gill filaments of the animal to be tested are not dry. Then cover the surface with a rigid polyurethane board having a density of 30 g / cm 3 and a thickness of 2 mm to complete the fixation of Macrobrachium rosenbergii.

[0061] S04. Send the fixed animal to be tested into an X-ray machine through an automated device or manually to collect a perspective view of the animal to be tested. The parameters of the X-ray machine are kvp: 40 mA: 80.00 ms: 16.00 mAs: 1.25.

[0062] S05. Collect the phenotypic data of the animal to be tested through image analysis software.

[0063] S06. Digitally archive the collected phenotypic data.

[0064] Comparative Example 2:

[0065] A method for collecting perspective phenotypic characteristics of aquatic animals is provided. In this embodiment, Macrobrachium rosenbergii of the same batch as in Example 1 is taken as an example. The entire collection system is carried out in an environment of 25°C ± 2°C. The specific steps include:

[0066] S01. Place the Macrobrachium rosenbergii to be tested in an aquarium system at room temperature and acclimate for ≥ 7 days.

[0067] S02. Place each Macrobrachium rosenbergii into the container described in Example 1.

[0068] S03. Wrap and cover the animal to be tested with rigid polyurethane particles having the same density as in Example 1, i.e., 30 g / cm 3 , a particle size of 1 mm. Add an appropriate amount of water to ensure that the gill filaments of the animal to be tested are not dry. Then cover the surface with a rigid polyurethane board having a density of 30 g / cm 3 and a thickness of 2 mm to complete the fixation of Macrobrachium rosenbergii.

[0069] S04. Send the fixed animal to be tested into an X-ray machine through an automated device or manually to collect a perspective view of the animal to be tested. The X-ray machine parameters are kvp: 40 mA: 80.00 ms: 16.00 mAs: 1.25.

[0070] S05. Collect the phenotypic data of the animal to be tested through image analysis software.

[0071] S06. Digitally archive the collected phenotypic data.

[0072] Comparative Example 3:

[0073] Provide a method for collecting phenotypic characteristics of aquatic animals by applying a low-temperature treatment method. Obtain Macrobrachium rosenbergii, an aquatic animal of the same batch as in Example 1. Use the same X-ray machine and parameters as in Example 1 to obtain a perspective view. Before sending it into the X-ray machine, place Macrobrachium rosenbergii in cold water at 4°C and let it stand still for 10 - 20 s, then place it in the X-ray machine and wait for it to stand still to obtain a perspective view.

[0074] Comparative Example 4:

[0075] Provide a method for collecting phenotypic characteristics of aquatic animals by applying a stress of being out of water. Obtain Macrobrachium rosenbergii, an aquatic animal of the same batch as in Example 1. Use the same X-ray machine and parameters as in Example 1 to obtain a perspective view. Before sending it into the X-ray machine, let Macrobrachium rosenbergii stand still out of water for 10 - 15 s, then place it in the X-ray machine and wait for it to stand still to obtain a perspective view.

[0076] Experimental Example:

[0077] Respectively count the number of broken whiskers, the number of dead individuals, and the average time per individual of Macrobrachium rosenbergii after the perspective collection of the phenotypic characteristics of aquatic animals in Example 1, Example 2, and Comparative Examples 1 - 4. The statistical results are shown in Table 1.

[0078] Table 1. Statistics on the health status of shrimp during the collection process

[0079] Example group Total number of shrimps / each Number of shrimps with broken whiskers / each Number of dead shrimps / each Average time per shrimp / s Example 1 200 5 0 11.2 Example 2 200 8 0 8.5 Comparative example 1 200 14 2 13.8 Comparative example 2 200 22 5 16.5 Comparative example 3 200 32 10 18.4 Comparative example 4 200 45 13 22.0

[0080] As can be seen from Table 1, when high-throughput collection is carried out, the high efficiency and low stimulation of the solution of the present application can be manifested. When traditional methods are used to perform low-temperature treatment or water separation stress on shrimp, it is easy to cause the broken whiskers and death of shrimp. The mortality rate is not less than 5%, indicating that the low-temperature treatment and water separation stress have a greater stimulation on shrimp, and it is easy to cause a large stress response, resulting in broken whiskers or even death. Moreover, the above two methods take a long time and are not conducive to high-throughput applications. In addition, when collecting by simply covering the animal to be tested with sponge particles with a lower hardness or rigid polyurethane particles with a higher hardness, it may also cause the breakage of shrimp whiskers and the death of shrimp. And selecting a single material to wrap aquatic animals cannot significantly increase the sedation time of aquatic animals, so the average collection time is increased compared with mixing two kinds of particles with different hardness, which is not conducive to high-throughput collection.

[0081] The method of the present application is conducive to quickly fixing aquatic animals by selecting porous soft materials with similar particle sizes, similar densities but different soft hardnesses to wrap aquatic animals, and has less stimulation to aquatic animals, thus greatly reducing the stress response. Therefore, the broken whisker rate of shrimp can be reduced to less than 5%, and no death of shrimp occurs. The average time per individual is significantly lower than that of low-temperature treatment or water separation stress and other methods, which is conducive to improving the perspective collection efficiency. When applied to the high-throughput collection process, it can significantly save time and cost. Completing high-throughput collection in a short time further reduces the stress response of aquatic animals and improves the accuracy of phenotypic characteristic collection.

[0082] Example 3:

[0083] The prior art has a Chinese patent application for invention with the publication number CN107144223A, which discloses a shrimp measurement system and its measurement method. This patent application specifically discloses collecting the side image of a live shrimp by using a camera and obtaining the corresponding growth phenotypic parameters of the shrimp based on the image. However, when implementing according to its technical solution, when the central axis of the shrimp's body from head to tail is not perpendicular to the camera lens on one side, that is, when the shrimp body is tilted to the left or right side, or when the shrimp body is bent and the head of the shrimp is significantly raised, there will be a deviation between the body length obtained according to the distance between the two vertical lines in the image and the actual body length, resulting in measurement errors. In fact, the posture of aquatic animals, especially shrimp, has a great influence on the collection of their phenotypic characteristics. Although the perspective collection method of the present application can easily obtain various indicators of shrimp, there will still be certain errors in the phenotypic characteristics measured when the posture of shrimp is fully stretched and when it is curled up. Therefore, making the shrimp quickly maintain a straight and stretched posture during the collection process has a positive effect on improving the accuracy of high-throughput collection.

[0084] The inventor accidentally discovered in experimental research that although light has little impact on the acquisition of X-ray machines, the presence and direction of light in the container are relevant to the posture of the shrimp. Therefore, the inventor improved the container, specifically including: a hole that allows light to pass through but does not allow water to pass through is opened on the surface of the container on the side of the head of the aquatic animal, and the diameter of the hole gradually increases from bottom to top. In this specific embodiment, the container is as Figure 7 shown. The diameters of the holes from bottom to top are 1mm, 2mm, 4mm, 6mm, 8mm, and 10mm respectively. The specific implementation solution can be to open holes on the surface of the container, fill the container with a plastic bag inside the container, and then use this container to complete the perspective acquisition of aquatic animals.

[0085] Referring to the solution of the prior art, the posture of the shrimp is judged whether it is in a straight and extended state through the side image, specifically as Figure 8 shown. First, determine the cephalothorax boundary point D, calibrate the straight line L1 from the cephalothorax boundary point D towards the eye direction, and the straight line L1 is tangent to the upper edge of the cephalothorax; calibrate the straight line L2 from the cephalothorax boundary point D towards the tail direction, and the straight line L2 is tangent to the highest points of abdominal segment 1, abdominal segment 2, and abdominal segment 3; take the midpoint of abdominal segment 6 and calibrate the straight line L3 towards the eye direction, and L3 is tangent to the upper edge of at least one of abdominal segment 3, abdominal segment 4, and abdominal segment 5. The standard for the shrimp to be in a straight and extended state is that the straight lines L1 and L2 form an obtuse angle 1, the straight lines L2 and L3 form an obtuse angle 2, and the obtuse angle 1 is between 145° - 160°, and the obtuse angle 2 is between 135° - 160°.

[0086] The solution of this embodiment is adopted to repeat Embodiment 2, with the difference that the container with holes described in this embodiment is used. During the process, only the phenotypic characteristics of Macrobrachium rosenbergii are collected by fluoroscopy after being tranquilized, that is, whether the posture of the shrimp is straight and stretched is not considered. 100 shrimps are measured, and the average time per single shrimp is 8.8 s, which is basically the same as that in Embodiment 2. Randomly select 20 X-ray films of Macrobrachium rosenbergii from Embodiment 2 and this embodiment respectively, and count the angles of obtuse angle 1 and obtuse angle 2 according to the method described in this embodiment. The average value of obtuse angle 1 in Embodiment 2 is 144.5°, and the average value of obtuse angle 2 is 138.1°; the average value of obtuse angle 1 in this embodiment is 153.6°, and the average value of obtuse angle 2 is 151.8°. The statistical results show that using a container with holes to hold Macrobrachium rosenbergii helps to promote its body stretching. The possible reason is that Macrobrachium rosenbergii is in water, and there is unequal light above and below in front of it, giving it an illusion that it is still in the water area, which is beneficial to its body stretching. On the premise of not significantly affecting the collection efficiency, an image of the shrimp with a straight and stretched posture is obtained. The straight and stretched shrimp body is also convenient for the later collection of phenotypic data and improving the operation accuracy of the phenotypic data collection model, thereby improving the consistency and accuracy of the data, and solving the problem in the prior art that to obtain phenotypic data, the measurer needs to keep the shrimp body straight and stretched by hand and then take pictures.

[0087] The conventional technologies in the above embodiments are the prior arts well-known to those skilled in the art, so they will not be described in detail here.

[0088] The specific embodiments described herein are merely illustrative of the spirit of the present invention. Those skilled in the art to which the present invention pertains can make various modifications or supplements to the described specific embodiments or use similar ways to replace them, but will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

[0089] Although a detailed description of the present invention has been made and some specific embodiments have been cited, it is obvious that various changes or modifications can be made by those skilled in the art as long as they do not depart from the spirit and scope of the present invention.

[0090] While the foregoing specific embodiments have shown, described, and pointed out the novel features applied to various embodiments, it should be understood that various omissions, substitutions, and changes in the form and details of the illustrated apparatus or method may be made without departing from the spirit of the present disclosure. Additionally, the various features and methods described above may be used independently of one another or may be combined in various ways. All possible combinations and sub-combinations are intended to fall within the scope of the present disclosure. Many of the above embodiments include similar components, and thus, these similar components may be interchangeable in different embodiments. Although the present invention has been disclosed in the context of certain embodiments and examples, those skilled in the art should understand that the present invention may extend beyond the specifically disclosed embodiments to other alternative embodiments and / or applications and their obvious modifications and equivalents. Therefore, the present invention is not intended to be limited by the specific disclosure of the preferred embodiments herein.

[0091] Matters not described in detail in this invention are all well-known technologies.

Claims

1. A method for perspective acquisition of phenotypic characteristics of aquatic animals, characterized in that Comprising: Using porous soft materials with similar particle sizes, similar densities but different softness and hardness to wrap aquatic animals and adding water, and the density of the porous soft materials is lower than the density of the aquatic animals, fixing them so as not to affect image acquisition and not to affect the life of the animals, and using an X-ray machine to collect perspective views of the aquatic animals to be tested; The porous soft materials with similar particle sizes, similar densities but different softness and hardness specifically include: Sponge particles with a density of 29 g / cm 3 and a 25% compression hardness of 1.9 Kpa; and Hard polyurethane particles with a density of 28 - 30 g / cm 3 .

2. The method for collecting perspective of phenotypic characteristics of aquatic animals according to claim 1, wherein: The similar particle sizes mean that the particle size difference of the porous soft materials is not higher than 10%.

3. The method for perspective acquisition of phenotypic characteristics of aquatic animals according to claim 1, characterized in that: The similar densities mean that the density difference of the porous soft materials is not higher than 10%.

4. The method for perspective acquisition of phenotypic characteristics of aquatic animals according to claim 1, wherein: The particle size diameter of the porous soft materials is 0.5 - 5 mm.

5. The method for perspective acquisition of phenotypic characteristics of aquatic animals according to claim 1, wherein: The aquatic animals are temporarily cultured in an aquarium system at room temperature for ≥ 7 days before being wrapped with porous soft materials.

6. The method for perspective acquisition of phenotypic characteristics of aquatic animals according to claim 1, wherein: The aquatic animals include shrimps.

7. The method for perspective acquisition of phenotypic characteristics of aquatic animals according to claim 1, wherein: Before using an X-ray machine to collect perspective views of the animals to be tested, covering and fixing the aquatic animals to be tested with porous soft materials of high hardness.

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