A cell fluid storage device

Abstract

The present application relates to the technical field of biological medicine, in particular to a cell liquid storage device, which comprises a box body, a box cover matched with the box body, and a foldable cell bag arranged in the box body; a bag mouth is connected to a top of the cell bag, two conductive PC films are connected to inner walls of the bag mouth, the box body is connected with a first terminal, a second terminal and a third terminal, the first terminal and the second terminal both vertically penetrate the bag mouth and respectively abut against the two conductive PC films, an irreversible electrochromic label is connected in series between the second terminal and the third terminal, a bipolar plate is arranged on a front side wall of the box body, a battery is arranged on a front side wall of the bipolar plate, and the first terminal, the battery and the third terminal are connected in series in sequence.The present application is simple to assemble, has high space utilization, can determine that the cell bag has been filled with cell liquid according to discoloration of the irreversible electrochromic label, avoids repeated use of the cell bag, adopts contactless detection of the irreversible electrochromic label, avoids pollution of the cell liquid, and is safe and reliable.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a cell fluid storage device. Background Technology

[0002] In the field of biomedicine, cell bags are commonly used as containers for storing cell fluid. However, current mainstream cell bag products have low volume utilization when used in confined spaces, making them unsuitable for applications requiring higher capacity within limited spaces.

[0003] Cell bags are disposable and must be discarded after use. For detecting the presence of cell fluid in cell bags, the test strip method is a simple and commonly used approach. The test strip uses a chemical fluorescent dye or indicator that changes color based on changes in specific components or properties of the cell fluid. By observing the color change of the test strip, a preliminary determination of the presence of cell fluid can be made. However, the test strip method has the following drawbacks:

[0004] 1. Universality issue: Chemical fluorescent dyes or indicators can only target a specific type of cell sap and cannot meet the needs of determining all cell sap types;

[0005] 2. Storage time issue: If there is no liquid in the cell bag for the test strip to detect, the test strip cannot provide accurate results, which may lead to incorrect judgment;

[0006] 3. Contamination issues: When the test strip comes into contact with the cell bag, external contaminants may be introduced, affecting subsequent experiments or applications. Summary of the Invention

[0007] The purpose of this invention is to provide a cell fluid storage device to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a cell fluid storage device, comprising a box body, a box cover adapted to the box body, and a plurality of foldable cell bags disposed within the box body;

[0009] The cell bag has a mouthpiece fixedly connected to its top. Two conductive PC films are fixedly connected to the inner wall of the mouthpiece. A first terminal, a second terminal, and a third terminal are fixedly connected to the front side wall of the box. The first terminal and the second terminal both extend vertically upward through the mouthpiece and abut against the two conductive PC films respectively. An irreversible electrochromic label is connected in series between the second terminal and the third terminal. A bipolar plate is provided on the front side wall of the box. A battery is fixedly provided on the front side wall of the bipolar plate. The first terminal, the battery, and the third terminal are connected in series in sequence.

[0010] Optionally, the upper surface of the front side wall of the box body is provided with a slot, the lower surface of the box lid is integrally formed with a card plate that matches the slot, the card plate is provided with a second arc-shaped groove that matches the top outer edge of the bag mouth, and the front side wall of the box body and below the second arc-shaped groove is provided with a first arc-shaped groove.

[0011] Optionally, the lower surface of the lid is integrally formed with a lid edge, and the outer wall of the lid edge is interference-fitted with the inner wall of the box.

[0012] Optionally, the first terminal and the second terminal have the same structure. The first terminal includes a wiring plate fixedly connected to the front side wall of the box, an arc-shaped conductive sheet fixedly disposed in the first arc-shaped groove and matching the bottom outer edge of the bag mouth, and a conductive post fixedly disposed on the upper surface of the arc-shaped conductive sheet; the bottom outer edge of the bag mouth is provided with a positioning hole that runs through from top to bottom and matches the conductive post.

[0013] Optionally, the irreversible electrochromic label includes a transparent sheath, a graphene layer fitted inside the transparent sheath, a polydiyne color-changing layer coated on the upper surface of the graphene layer, and two first terminals fixedly penetrating the transparent sheath and electrically connected to the graphene layer; the two first terminals are respectively movably plugged into the terminal block of the second terminal and the third terminal.

[0014] Optionally, a second terminal and a third terminal are fixedly connected to the rear side wall of the bipolar plate. The second terminal is movably inserted into the terminal block, and the end of the third terminal away from the first terminal is movably inserted into the third terminal.

[0015] Optionally, both the cell bag and the bag mouth are made of EVA material, and the cell bag is bonded to the bag mouth as one piece by a high-frequency heat sealing process.

[0016] Optionally, the cell bag has a folding line on its width-direction sidewall, with the two ends of the folding line extending horizontally to the top and bottom of the cell bag, respectively, and the EVA membrane located in the width direction of the cell bag has five folding lines.

[0017] Optionally, the cell bag is bonded to the bag mouth as a whole by a high-frequency heat sealing process. The cell bag is made by the following steps: First, a cuboid fixture is placed on the EVA film, and the long and short sides of the EVA film are folded to fit against the outer surface of the cuboid fixture. Then, the folded edges are heat sealed by a radio frequency heat sealing process. After the heat sealing is completed, the fixture is removed, and the excess heat-sealed edges are trimmed. Finally, the above steps are repeated to heat seal the four sides in sequence to make an open cuboid-shaped cell bag.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. This invention is simple to assemble, has high space utilization, and can determine that the cell bag has been filled with cell fluid based on the color change of the irreversible electrochromic label, avoiding the reuse of cell bags. It has good versatility, and the irreversible electrochromic label adopts non-contact detection to avoid cell fluid contamination, making it safe and reliable.

[0020] 2. This invention constrains the position of the bag mouth by inserting conductive posts into the positioning holes, preventing the bag mouth from moving forward or backward during the filling process, thus improving filling stability. Furthermore, the bipolar plate can be detachably and fixedly installed on the box body through the movable connection of the second and third terminals with the terminal block, making assembly simple. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the present invention;

[0022] Figure 2 This is an assembly diagram of the box body and the box lid in this invention;

[0023] Figure 3 This is a schematic diagram of the cell bag structure in this invention;

[0024] Figure 4 This is a schematic diagram of the structure of the bag mouth, the first terminal, the second terminal, the third terminal, and the irreversible electrochromic label in this invention;

[0025] Figure 5 This is a partial cross-sectional view of the bag mouth, the first terminal, and the second terminal in this invention;

[0026] Figure 6 This is a partial cross-sectional view of the irreversible electrochromic label in this invention;

[0027] Figure 7 This is a schematic diagram of the bipolar plate in this invention.

[0028] In the diagram: 100, Box body; 101, Card slot; 102, First arc-shaped groove; 200, Box cover; 201, Card plate; 202, Second arc-shaped groove; 203, Cover edge; 300, Cell bag; 301, Bag mouth; 302, Conductive PC film; 303, Positioning hole; 304, Easy-break wire; 400, First terminal; 401, Terminal block; 402, Arc-shaped conductive sheet; 403, Conductive post; 500, Second terminal; 600, Third terminal; 700, Irreversible electrochromic label; 701, First terminal; 702, Transparent sheath; 703, Graphene layer; 704, Polydiyne color-changing layer; 800, Bipolar plate; 801, Second terminal; 802, Third terminal; 900, Battery. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] Example: Please refer to Figures 1 to 3 This invention provides a cell fluid storage device, including a box body 100, a lid 200 adapted to the box body 100, and two foldable cell bags 300 disposed inside the box body 100. The lower surface of the lid 200 has an integrally formed lid edge 203. Through an interference fit between the outer wall of the lid edge 203 and the inner wall of the box body 100, the box body 100 and the lid 200 can be stably connected as a whole, achieving closed storage of the two cell bags 300 and ensuring the integrity of the cell bags 300.

[0031] Based on the above embodiments, in this embodiment, the top of the cell bag 300 is fixedly connected to a spout 301. Both the cell bag 300 and the spout 301 are made of EVA material, and the cell bag 300 is bonded to the spout 301 as a whole by a high-frequency heat sealing process. A slot 101 is provided on the upper surface of the front side wall of the box body 100, and a card plate 201 that matches the slot 101 is integrally formed on the lower surface of the box cover 200. The card plate 201 has a second arc-shaped groove 202 that matches the top outer edge of the spout 301. A first arc-shaped groove 102 is provided on the front side wall of the box body 100 below the second arc-shaped groove 202. The space enclosed by the first arc-shaped groove 102 and the second arc-shaped groove 202 accommodates the spout 301 to extend out of the box body 100.

[0032] Please see Figure 3 The cell bag 300 has a folding line 304 on its width-direction sidewall, with both ends of the folding line 304 extending horizontally to the top and bottom of the cell bag 300, respectively. The cell bag 300 is bonded to the bag mouth 301 as a single unit using a high-frequency heat sealing process. The cell bag 300 is manufactured using the following steps: First, a cuboid fixture is placed on the EVA film, and the long and short sides of the EVA film are folded to fit against the outer surface of the cuboid fixture. Next, the folded edges are heat-sealed using a radio frequency heat sealing process. After heat sealing, the fixture is removed, and then the excess heat-sealed edges are trimmed. Finally, the above steps are repeated to heat seal all four sides in sequence, forming an open cuboid-shaped cell bag 300.

[0033] Before the radio frequency (RF) heat sealing process, the EVA membrane in the width direction of the cell bag has five fold lines 304. These five fold lines 304 give the EVA membrane a six-layer continuous fold structure in the width direction of the cell bag. During the RF heat sealing process, the first and second layers of the EVA membrane need to be welded together, as well as the fifth and sixth layers. After RF heat sealing, the cross-section of the EVA membrane becomes W-shaped. When the cell bag 300 is filled with liquid, the EVA membrane can expand and unfold near the third and fourth layers.

[0034] This invention uses a flexible fold 304 to compress two cell bags 300 within the box 100. The space enclosed by the box 100 and the lid 200 allows one cell bag 300 to expand to its maximum size, while the other cell bag 300 remains unexpanded. When one cell bag 300 is full of cell solution, cell solution can be poured into the other cell bag 300. During the filling process, the other cell bag 300 expands and compresses the already filled cell bag 300, forcing it to automatically discharge the cell solution, thus achieving cell solution transfer. This effectively utilizes the space of the enclosed box 100, achieving maximum liquid capacity and high space utilization.

[0035] Please see Figure 1 , Figure 4 and Figure 5 Based on the above embodiments, in this embodiment, two conductive PC films 302 are fixedly connected to the inner wall of the bag opening 301, and a first terminal 400, a second terminal 500, and a third terminal 600 are fixedly connected to the front side wall of the box body 100. The first terminal 400 and the second terminal 500 both penetrate vertically upward through the bag opening 301 and abut against the two conductive PC films 302 respectively. An irreversible electrochromic label 700 is connected in series between the second terminal 500 and the third terminal 600. A bipolar plate 800 is provided on the front side wall of the box body 100, and a battery 900, which is a lithium battery, is fixedly connected to the front side wall of the bipolar plate 800 by bolts. The first terminal 400, the battery 900, and the third terminal 600 are connected in series in sequence.

[0036] During the filling of cell bag 300 with cell solution, the cell solution flows through two conductive PC membranes 302. Because the cell solution is conductive, the first terminal 400, the cell solution, the second terminal 500, the irreversible electrochromic label 700, the third terminal 600, and the battery 900 form a series circuit. The irreversible electrochromic label 700 changes color under the influence of current. Operators can determine whether the cell bag 300 has been filled with cell solution based on the color change of the irreversible electrochromic label 700, avoiding the reuse of the cell bag 300. Compared to the conventional test strip method for detecting whether the cell bag 300 has been filled with cell solution, the irreversible electrochromic label 700 is effective for a long time, and because neither the first terminal 400 nor the second terminal 500 comes into contact with the cell solution, contamination of the cell solution can be avoided.

[0037] Based on the above embodiments, the first terminal 400 and the second terminal 500 in this embodiment have the same structure. The first terminal 400 includes a wiring plate 401 fixedly connected to the front side wall of the housing 100, an arc-shaped conductive sheet 402 fixedly disposed in the first arc-shaped groove 102 and matching the bottom outer edge of the mouthpiece 301, and a conductive post 403 fixedly disposed on the upper surface of the arc-shaped conductive sheet 402; a positioning hole 303 is provided on the bottom outer edge of the mouthpiece 301, which runs vertically through and matches the conductive post 403. The present invention constrains the position of the mouthpiece 301 by inserting the conductive post 403 into the positioning hole 303, preventing the mouthpiece 301 from moving forward or backward during the filling process and improving filling stability.

[0038] Please see Figure 6 The irreversible electrochromic label 700 includes a transparent sheath 702, a graphene layer 703 fitted inside the transparent sheath 702, a polydiyne color-changing layer 704 coated on the upper surface of the graphene layer 703, and two first terminals 701 fixedly penetrating the transparent sheath 702 and electrically connected to the graphene layer 703. The polydiyne color-changing layer 704 can respond to currents up to 250mA. When the current flowing through the polydiyne color-changing layer 704 reaches 250mA, the polydiyne color-changing layer 704 changes color; when the current is disconnected, the polydiyne color-changing layer 704 retains its color. The two first terminals 701 are movably connected to the terminal block 401 of the second terminal 500 and the third terminal 600, respectively, allowing the irreversible electrochromic label 700 to be detached from the second terminal 500 and the third terminal 600 for easy replacement. In this invention, except for the cell bag 300, bag mouth 301, conductive PC film 302, and irreversible electrochromic label 700 which are not reusable, the rest can be reused, resulting in low cost.

[0039] Please see Figure 7A second terminal 801 and a third terminal 802 are fixedly connected to the rear side wall of the bipolar plate 800. The second terminal 801 is movably inserted into the terminal block 401, and the end of the third terminal 600 away from the first terminal 701 is movably inserted into the third terminal 802. The bipolar plate 800 can be detachably and fixedly installed on the housing 100 through the movable insertion of the second terminal 801 and the third terminal 802 into the terminal block 401, making assembly simple.

[0040] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell fluid storage device, characterized by: The utility model provides a cell culture box, including box body (100), the box cover (200) of being suitable with box body (100) and a plurality of set up in box body (100) and the foldable cell bag (300); The bag top of the cell bag (300) is fixedly connected with a bag spout (301), the inner wall of the bag spout (301) is fixedly connected with two conductive PC films (302), the front side wall of the box body (100) is fixedly connected with a first terminal (400), a second terminal (500) and a third terminal (600), the first terminal (400) and the second terminal (500) are vertically upward through the bag spout (301) and respectively abut with two conductive PC films (302), the second terminal (500) and the third terminal (600) are connected in series with an irreversible electrochromic label (700), the front side wall of the box body (100) is provided with a bipolar plate (800), the front side wall of the bipolar plate (800) is fixedly provided with a battery (900), the first terminal (400), the battery (900) and the third terminal (600) are connected in series. The first terminal (400) includes a wiring board (401) fixedly connected to the front side wall of the box body (100), the rear side wall of the bipolar plate (800) is fixedly connected with a second terminal (801) and a third terminal (802), the second terminal (801) is movably inserted with the wiring board (401), and the end of the third terminal (600) is movably inserted with the third terminal (802); the bipolar plate (800) is movably inserted with the second terminal (801) and the third terminal (802) and the wiring board (401), which can be detachably fixedly installed on the box body (100).

2. A cell fluid storage device according to claim 1, wherein: A clamping groove (101) is formed on the upper surface of the front side wall of the box body (100), a clamping plate (201) is integrally formed on the lower surface of the box cover (200) and matches the clamping groove (101), a second arc-shaped groove (202) is formed on the clamping plate (201) and matches the top outer edge of the bag spout (301), and a first arc-shaped groove (102) is formed on the front side wall of the box body (100) below the second arc-shaped groove (202).

3. A cell fluid storage device according to claim 2, wherein: A cover rail (203) is integrally formed on the lower surface of the box cover (200), and the outer wall of the cover rail (203) is interference-fitted with the inner wall of the box body (100).

4. The cell solution storage device of claim 2, wherein: The first terminal (400) and the second terminal (500) are identical in structure, the first terminal (400) further includes an arc-shaped conductive sheet (402) fixedly arranged in the first arc-shaped groove (102) and matching the bottom outer edge of the bag spout (301), and a conductive column (403) fixedly arranged on the upper surface of the arc-shaped conductive sheet (402); and a positioning hole (303) is formed on the bottom outer edge of the bag spout (301) and penetrates up and down and matches the conductive column (403).

5. A cell fluid storage device according to claim 4, wherein: The irreversible electrochromic label (700) comprises a transparent sheath (702), a graphene layer (703) sleeved in the transparent sheath (702), a polydiacetylene electrochromic layer (704) coated on the upper surface of the graphene layer (703), and two first connecting posts (701) fixed through the transparent sheath (702) and electrically connected with the graphene layer (703); the two first connecting posts (701) are respectively movably connected with the terminal plate (401) of the second terminal (500) and the third terminal (600).

6. The cell solution storage device of claim 1, wherein: The cell bag (300) and the bag mouth (301) are both made of EVA material, and the cell bag (300) is bonded with the bag mouth (301) into an integrated whole through a high-frequency heat sealing process.

7. A cell fluid storage device according to claim 6, wherein: The width direction side wall of the cell bag (300) is provided with a folding line (304), the two ends of the folding line (304) respectively extend to the bag top and the bag bottom of the cell bag (300) along the horizontal direction, and the EVA film in the width direction of the cell bag (300) is provided with five folding lines (304).

8. A cell fluid storage device according to claim 7, wherein: The cell bag (300) is bonded with the bag mouth (301) into an integrated whole through a high-frequency heat sealing process, and the cell bag (300) is manufactured by the following steps: Firstly, a cuboid tool is placed on the EVA film, and the long side and the short side of the EVA film are folded to be attached to the outer surface of the cuboid tool, secondly, a radio frequency heat sealing process is used to heat seal the attachment of the folded side, after the heat sealing is completed, the tool is taken out, then the excess heat sealing side is cut off, finally, the above steps are repeated, and four side edges are sequentially heat sealed to manufacture a cell bag (300) in the shape of an open cuboid.

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