A compartmentalized method for removing heavy metals by photoelectron-assisted microorganisms

Abstract

The present invention discloses a compartmentalized method for removing heavy metals by optoelectronic synergy with microorganisms, which includes: dissolving lecithin and flavin mononucleotide in a water bath with chloroform, evaporating to form a film by rotary evaporation under reduced pressure, performing a water bath after vacuum pumping; adding a buffer solution containing MV, completely dissolving the lecithin on the wall, transferring it to a polyethylene tube, vortexing, then freezing it in a liquid nitrogen tank, and then thawing it in a water bath pot, repeating multiple times, dialyzing the thawed solution for a certain period of time, and obtaining a liposome compartment added with flavin mononucleotide when the absorbance of the dialysate coincides with that of the buffer solution; adding titanium nitride semiconductor and wet cells of Alcaligenes faecalis to the liposome compartment, irradiating the compartment with a xenon lamp, and forming an optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system in the liposome compartment. The present invention establishes an optoelectron + FMN + wet cells of Alcaligenes faecalis + U(VI) system by adding FMN to the liposome compartment, improving the reduction and removal ability of U(VI).

Description

Technical Field

[0001] The present invention belongs to the technical field of wastewater treatment. More specifically, the present invention relates to a compartmentalized method for removing heavy metals by using optoelectronic synergistic microorganisms. Background Art

[0002] With the continuous rapid development of the economy, environmental pollution problems have attracted more and more attention, especially heavy metal pollution problems. Due to their strong toxicity, non-degradability, easy enrichment, ability to be transmitted in the food chain, and carcinogenicity, heavy metals pose potential threats to the ecological environment and human health. In industrial production processes such as electroplating, leather making, anti-corrosion, and dyeing, a large amount of heavy metal wastewater is discharged into water bodies such as rivers. The treatment of heavy metal polluted wastewater has attracted widespread attention. Currently, the main methods for treating heavy metal wastewater include ion exchange method, chemical precipitation method, electrochemistry method, adsorption method, etc.

[0003] The ion exchange method uses ion exchange resins or chelating resins as media to remove heavy metal ions in water. Ion exchange resins are mainly prepared from raw materials such as lignin, peat, and cellulose. Chelating resins not only have the advantages of general ion exchange resins but also have the characteristics of high selectivity. However, the ion exchange method and membrane separation method are simple to operate but costly, and may even be blocked by some fine suspended solid particles, and their efficiency is not high when treating low-concentration heavy metal wastewater.

[0004] The precipitation principle is to increase the pH value of the water body, so that heavy metals form various complexes with hydroxide ions or complexes in the form of carbonates, and separate them from the water. At the same time, heavy metal ions can also be removed from the water body by adding sulfur-containing compounds as precipitants to form precipitates. The removal of pollutants in the water body through flocculation is mainly used in conventional wastewater treatment. Using iron salts and aluminum salts as flocculants, synergistically combined with natural minerals with purification functions and modified to form high-performance flocculation materials to improve the efficiency of sewage treatment. However, the chemical precipitation method generally requires the use of a large amount of chemical reagents, which not only increases the cost but also easily generates sludge, causing secondary pollution to the environment.

[0005] The electrochemistry method refers to a series of reactions such as oxidation-reduction, decomposition, precipitation, and flotation under the action of an electric current to remove heavy metal ions and organic pollutants in wastewater. However, its electrode plates are consumed quickly, it absorbs a large amount of power, and the effect of removing low-concentration electroplating wastewater is not good. It is only applicable to the treatment of medium and small-sized electroplating wastewater.

[0006] Adsorption is a traditional method for treating wastewater by using porous substances to adsorb pollutants in water. The most widely used and earliest one is activated carbon adsorption, which is widely used in water purification production. Currently, activated carbon fibers, molecular sieves, activated carbon, nano-carbon materials, etc. have been successively put on the market, and research and development have been focused on the surface modification technology of activated carbon and the improvement of water treatment equipment. At present, the research level on the surface of mineral adsorbents has reached the molecular level. In order to explore the high performance of environmental mineral materials, certain technical improvements are needed for natural minerals with ion exchange, adsorption, and filtration functions. Adsorption still has certain limitations in practical applications. For example, the current desorption methods are relatively single, the re-adsorption capacity of adsorbents treated by traditional desorption technologies is poor, the types of desorbents are scarce and vulnerable to the external environment, and it is not easy to separate and recover microorganisms after adsorbing heavy metals, etc.

[0007] Microbial adsorption is a newly emerging treatment method in recent years and can achieve relatively obvious effects in wastewater treatment. Microbial adsorption means that heavy (like) metals in the medium are adsorbed and removed through the chemical composition or structural characteristics of microorganisms themselves or their products, and then the solid-liquid separation is carried out to reduce the concentration of metal ions in water. Compared with other wastewater treatment methods, the microbial adsorption method has a faster treatment rate for wastewater, and the sources of its raw materials are relatively wide, which can meet the requirements of relevant technical personnel. Compared with other wastewater treatment methods, this treatment method will not cause secondary pollution, so it has a broad development prospect in the future. There have been many reports at home and abroad on using microorganisms to reduce or eliminate heavy metal pollution. Microorganisms cannot degrade and destroy metals, but can affect the migration and transformation of metals in the environment by changing their chemical or physical properties. Currently, there are many studies on using microorganisms to adsorb radionuclides. For example, some studies have shown that the adsorption capacity of 1 g of dry weight yeast for U can reach 3000 mg.

[0008] Photocatalytic reduction mainly relies on semiconductor metal oxides as photocatalysts to remove pollutants by undergoing redox reactions with pollutants, and has the advantages of high efficiency, environmental protection, and no secondary pollution, etc. Since Lu Anhua et al. discovered that the photoelectrons of some semiconductor minerals can promote the growth and metabolism of some microorganisms, this provides the possibility for the photoelectric synergistic reduction of heavy metal ions by microorganisms. Because in nature, semiconductor minerals and microorganisms coexist in the surface system. However, its regulation method and action mechanism have not been clearly explained. In addition to reducing metal ions, photoelectrons can also degrade organic pollutants, thus having a synergistic advantage that cannot be achieved by other methods.

[0009] However, there is no mention in the existing technology of using flavin mononucleotide (FMN) for the photoelectric synergistic removal of heavy metals by microorganisms. Summary of the Invention

[0010] An object of the present invention is to solve at least the above problems and / or deficiencies and provide at least the advantages described hereinafter.

[0011] To achieve these objects and other advantages in accordance with the present invention, a compartmentalized method for removing heavy metals using optoelectronic synergistic microorganisms is provided, including:

[0012] Preparing a liposome compartment using chloroform, lecithin, flavin mononucleotide, a buffer solution containing heavy metals and MV, adding titanium nitride semiconductor and wet cells of Alcaligenes faecalis to the liposome compartment, performing light irradiation, and forming an optoelectronic + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system in the liposome compartment, thereby reducing heavy metals and achieving the removal of heavy metals.

[0013] Preferably, specifically, it includes the following steps:

[0014] Step 1: Dissolve lecithin and flavin mononucleotide in chloroform in a water bath at a certain temperature. After the lecithin is completely dissolved, evaporate to form a film under reduced pressure, evacuate, and then perform a water bath.

[0015] Step 2: After evaporation, pour the buffer solution containing heavy metals and MV into the container in Step 1, completely dissolve the lecithin on the wall, transfer it to a polyethylene tube, vortex for a certain time, then freeze it in a liquid nitrogen tank, and then thaw it in a water bath at a certain temperature. Repeat multiple times, and then put the thawed solution into a dialysis bag and dialyze for a certain time. When the absorbance of the dialysis solution coincides with that of the buffer solution, a liposome compartment added with flavin mononucleotide is obtained.

[0016] Step 3: Add titanium nitride semiconductor and wet cells of Alcaligenes faecalis to the liposome compartment obtained in Step 2, and irradiate the liposome compartment with a xenon lamp to generate optoelectrons by the titanium nitride semiconductor, forming an optoelectronic + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system in the liposome compartment, thereby reducing U(VI) and achieving the removal of U(VI).

[0017] Preferably, in Step 1, the mass-volume ratio of chloroform, lecithin, and flavin mononucleotide is 5 - 40 mL : 15 - 120 mg : 0.1 - 30 mg.

[0018] Preferably, in Step 1, the water bath dissolution temperature of lecithin and flavin mononucleotide is 40 - 80°C.

[0019] Preferably, in step two, the buffer solution of MV is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution, the concentration of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid is 5-30 mM, and 20-100 ppm of uranium is dissolved in the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution; the volume ratio of the buffer solution of MV to chloroform is 1-10 mL : 5-40 mL.

[0020] Preferably, the pH of the buffer solution of MV is 4-6.

[0021] Preferably, in step two, the vortex time is 1-12 h, the freezing temperature in the liquid nitrogen tank is -190~-80 °C, and the freezing time is 2-8 h.

[0022] Preferably, in step two, the thawing temperature in the water bath is 50-70 °C, and the dialysis time is 50-120 h.

[0023] Preferably, in step three, the concentration of wet cells of Alcaligenes faecalis is 1-5 g / L; the mass-volume ratio of titanium nitride semiconductor, wet cells of Alcaligenes faecalis to chloroform is 0.5-2 mg : 0.2-1.5 mg : 5-40 mL.

[0024] An application of a compartmentalized method for removing heavy metals by photoelectron-assisted microorganisms, wherein the compartmentalized method for removing heavy metals by photoelectron-assisted microorganisms is applied to the reduction and removal of uranium in wastewater.

[0025] In order to improve the reduction and extraction ability of uranium in the photoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system, modified titanium nitride semiconductor is used to replace titanium nitride semiconductor, and the preparation method of the modified titanium nitride semiconductor includes:

[0026] S1. Dissolve nickel chloride in naphthalene tetracarboxylic dianhydride, filter and dry to obtain the Ni-MOF precursor, wherein the mass-volume ratio of nickel chloride to naphthalene tetracarboxylic dianhydride is 0.05-0.12 g : 1 mL;

[0027] S2. Mix the Ni-MOF precursor with the ethanol solution of tetrabutyl titanate, react at 150-280 °C, cool, filter, wash and dry after the reaction, and obtain the intermediate product after firing in air at 250-550 °C; wherein, the volume ratio of the Ni-MOF precursor to the ethanol solution of tetrabutyl titanate is 1:2-5, and the mass fraction of tetrabutyl titanate in the ethanol solution of tetrabutyl titanate is 5-30%;

[0028] S3. Mix titanium dioxide powder with a particle size distribution in the range of 10 - 500 nm with dopamine hydrochloride solution, react in the dark, filter and dry to obtain modified titanium dioxide powder. The reaction time is 8 - 72 h, the reaction temperature is 5 - 30 °C, the mass - volume ratio of titanium dioxide powder to dopamine hydrochloride solution is 1 - 2 mg : 1 - 3 mL, and the concentration of dopamine hydrochloride solution is 1 - 5 mg / mL;

[0029] S4. After mixing the intermediate product, modified titanium dioxide powder, and dicyandiamide, calcine them at a heating rate of 10 °C / min in a nitrogen atmosphere at 500 - 980 °C for 5 - 12 h, and keep the temperature for 5 h to obtain modified semiconductor titanium nitride; among them, the mass ratio of the intermediate product, modified titanium dioxide powder, and dicyandiamide is 1 - 4 : 1 : 10 - 20.

[0030] The present invention has at least the following beneficial effects: The present invention establishes a photoelectron + flavin mononucleotide (FMN) + wet cells of Alcaligenes faecalis + U(VI) system by adding flavin mononucleotide (FMN) to the liposome compartment, which improves the reduction and removal ability of U(VI).

[0031] The present invention further improves the removal effect of the photoelectron + flavin mononucleotide (FMN) + wet cells of Alcaligenes faecalis + U(VI) system on U(VI) by using modified semiconductor titanium dioxide.

[0032] Other advantages, objectives, and features of the present invention will be partially reflected by the following description and partially understood by those skilled in the art through the research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the photoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared by the present invention;

[0034] Figure 2 It is a time - current curve graph of the blank liposome system prepared in Comparative Example 1;

[0035] Figure 3 It is a time - current curve graph of the photoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared in Example 1 of the present invention;

[0036] Figure 4 It is a Nyquist impedance fitting spectrum of the photoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared in Example 1 of the present invention and the blank liposome system of Comparative Example 1;

[0037] Figure 5Absorbance change over time graphs for the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared in Example 1 and the blank lipid system of Comparative Example 1;

[0038] Figure 6 Absorbance change over time graphs for the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) systems prepared in Example 1 and Example 2. Detailed implementation manners

[0039] The following further elaborates the present invention in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the text of the specification.

[0040] It should be understood that terms such as "having", "comprising", and "including" as used herein do not exclude the presence or addition of one or more other elements or their combinations.

[0041] Example 1

[0042] This example provides a compartmentalized method for removing heavy metals using optoelectrons in cooperation with microorganisms, including the following steps:

[0043] Step 1: Dissolve 20 mL of chloroform, 60 mg of lecithin, and 5 mg of flavin mononucleotide in a 55°C water bath. After the lecithin is completely dissolved, evaporate to form a film under reduced pressure, evacuate, and then perform a water bath.

[0044] Step 2: After rotary evaporation, pour 6 mL of the MV buffer solution into the round-bottom flask in Step 1 to completely dissolve the lecithin on the wall, transfer it to a polyethylene tube, vortex for 2 h, then place it in a liquid nitrogen tank for freezing. The freezing temperature is -120°C and the freezing time is 2 h. Then thaw it in a 60°C water bath and repeat 10 times. Put the prepared solution into a dialysis bag and change the dialysis solution every 6 h. Dialyze the thawed solution for 100 h. When the absorbance of the dialysis solution matches that of the buffer solution, a lipidosome compartment added with flavin mononucleotide is obtained; among them, the buffer solution is 6 mL of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution, where the concentration of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid is 10 mM, and 50 ppm of uranium is dissolved in the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution. The pH of the MV buffer solution is 4.2.

[0045] Step 3: Add 1 mg of semiconductor titanium nitride and 1.5 mg of wet cells of Alcaligenes faecalis to the lipidosome compartment obtained in Step 2, and irradiate the compartment with a xenon lamp to cause the semiconductor titanium nitride to generate optoelectrons, forming an optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system in the lipidosome compartment.

[0046] Example 2

[0047] This embodiment provides a compartmentalized method for removing heavy metals using optoelectronic synergistic microorganisms. In step three, the added material is modified semiconductor titanium nitride, and the rest of the method is the same as that of Example 1. The preparation method of the modified semiconductor titanium nitride includes:

[0048] S1. Dissolve 5 g of nickel chloride in 100 mL of naphthalene tetracarboxylic dianhydride, filter and dry to obtain the Ni-MOF precursor;

[0049] S2. Mix 20 mL of the Ni-MOF precursor with 40 mL of an ethanol solution of tetrabutyl titanate, where the mass fraction of tetrabutyl titanate is 20%. React at 150 °C, cool, filter, wash, and dry after the reaction. After calcining in air at 400 °C, obtain the intermediate product;

[0050] S3. Mix 20 g of titanium dioxide powder with a particle size distribution of 10 - 500 nm and 30 mL of a hydrochloric acid dopamine solution with a concentration of 3 mg / mL, react in the dark, filter and dry to obtain the modified titanium dioxide powder. The reaction time is 8 h, and the reaction temperature is 20 °C;

[0051] S4. Mix 8 g of the intermediate product, 2 g of the modified titanium dioxide powder, and 20 g of dicyandiamide, then calcine in a nitrogen atmosphere at 600 °C at a heating rate of 10 °C / min. The calcination time is 6 h, keep the temperature for 5 h, and cool to room temperature to obtain the modified semiconductor titanium nitride.

[0052] Comparative Example 1

[0053] This comparative example provides a preparation method of a blank liposome system, which does not add FMN, and the rest of the method is the same as that of Example 1.

[0054] It can be seen from the time-current curve that under light illumination conditions, the current in the optoelectronic + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared in Example 1 is 1 time larger than that under dark conditions, and is larger than the current in the blank liposome system in Comparative Example 1 ( Figure 2 ), indicating that adding FMN ( Figure 3 can accelerate the electron transfer.

[0055] Through the analysis of the alternating current impedance ( Figure 4 ), it can be known that the resistance value of the blank liposome prepared in Comparative Example 1 is 1447 Ω, and the resistance value of the system in Example 1 after adding FMN is 1224 Ω. Compared with the resistance value of the blank liposome system, it decreases by 223 Ω, indicating that the internal resistance of electron transfer in the system decreases after adding FMN, and the electron transfer speed increases.

[0056] To detect whether the lipid systems of each Example 1 and Comparative Example 1 can have a better effect in removing heavy metals, it is necessary to measure the concentration of uranium ions. According to the principle of "like dissolves like", 0.05% arsenazo III is configured as a complexing chromogenic agent with U(VI). The absorbance value of the complex after the reaction in Example 1 and Comparative Example 1 is measured at 652 nm using a Hitachi U-3900 ultraviolet spectrophotometer; 5 mL of the shaken liquid after the experimental reaction is pipetted into a centrifuge tube using a pipette gun, Triton X-100 is added to dissolve the liposomes, and after mixing, 1 mL of the supernatant is pipetted to measure the absorbance value. Before the test, the absorbance value is adjusted to 0 with a buffer solution, and according to the standard curve, the content of U(VI) in the solution after the reaction is calculated.

[0057] The change in the concentration of U(VI) after reduction by the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system was measured using the arsenazo III method, and at the same time, the blank liposome system of Comparative Example 1 was used as a control to obtain Figure 5 。

[0058] In the blank liposome system of Comparative Example 1, the concentration of U(VI) remained basically unchanged. In the liposome system synthesized by adding FMN in Example 1, the concentration of U(VI) decreased with the increase of time.

[0059] When the initial concentration of U(VI) was 50 ppm, the schematic diagram of the change in the concentration of U(VI) in the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system prepared in Example 1 and Example 2 was measured respectively to obtain Figure 6 ; it can be seen that the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system using modified titanium nitride semiconductor has a better removal effect on U(VI) than Example 1.

[0060] In the present invention, by synthesizing the optoelectron + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) system, and adding FMN with redox activity as a control to analyze the change in the concentration of U(VI) in the liposomes, the following main conclusions are obtained:

[0061] By electrochemically analyzing the current and resistance in the system of liposomes synthesized with or without FMN, it is concluded that the liposome system added with FMN has higher electrochemical activity, its resistance value is 1224 Ω, has lower internal resistance, and accelerates the electron transfer rate.

[0062] Photocatalytic reduction of the two liposomes, and the change in the concentration of U(VI) after reduction was measured by the arsenazo III method. As the action time increased, the concentration of U(VI) in the liposomes added with FMN gradually decreased, while the concentration of U(VI) in the blank liposomes hardly changed significantly.

[0063] The number of devices and the processing scale described herein are used to simplify the description of the present invention. Applications, modifications, and variations of the present invention will be apparent to those skilled in the art.

[0064] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and the embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the equivalent scope, the present invention is not limited to specific details and the illustrated examples described herein.

Claims

1. A compartmentalized method for removing heavy metals by using optoelectronic and microbial synergy, characterized in that Including: Preparing a liposome compartment using chloroform, lecithin, flavin mononucleotide, a buffer solution containing heavy metals and MV, adding titanium nitride semiconductor and wet cells of Alcaligenes faecalis to the liposome compartment, performing light irradiation, and forming a system of photoelectrons + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) in the liposome compartment, thereby reducing heavy metals and achieving the removal of heavy metals; Specifically including the following steps: Step 1: Dissolve lecithin and flavin mononucleotide in a water bath at a certain temperature using chloroform. After the lecithin is completely dissolved, perform rotary evaporation under reduced pressure to form a film, evacuate to vacuum, and then perform a water bath; Step 2: After rotary evaporation, pour the buffer solution containing heavy metals and MV into the container of Step 1 to completely dissolve the lecithin on the wall, transfer it to a polyethylene tube, vortex for a certain time, then place it in a liquid nitrogen tank for freezing, and then thaw it in a water bath at a certain temperature. Repeat multiple times, and then put the thawed solution into a dialysis bag for dialysis for a certain time. When the absorbance of the dialysis solution coincides with that of the buffer solution, a liposome compartment added with flavin mononucleotide is obtained; Step 3: Add titanium nitride semiconductor and wet cells of Alcaligenes faecalis to the liposome compartment obtained in Step 2, and irradiate the liposome compartment with a xenon lamp to enable the titanium nitride semiconductor to generate photoelectrons, forming a system of photoelectrons + flavin mononucleotide + wet cells of Alcaligenes faecalis + U(VI) in the liposome compartment, thereby reducing U(VI) and achieving the removal of U(VI); The buffer solution of MV is a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution.

2. The compartmentalized method for removing heavy metals by using optoelectronic and microbial synergy according to claim 1, wherein, In the said Step 1, the mass-volume ratio of chloroform, lecithin, and flavin mononucleotide is 5 - 40 mL : 15 - 120 mg : 0.1 - 30 mg.

3. The compartmentalized method for removing heavy metals by optoelectronic synergistic microorganisms according to claim 2, wherein In the said Step 1, the water bath dissolution temperature of lecithin and flavin mononucleotide is 40 - 80 °C.

4. The compartmentalized method for removing heavy metals by using optoelectronic cooperation with microorganisms according to claim 1, characterized in that In the said Step 2, the concentration of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid is 5 - 30 mM, and 20 - 100 ppm of uranium is dissolved in the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid solution; the volume ratio of the buffer solution of MV to chloroform is 1 - 10 mL : 5 - 40 mL.

5. The compartmentalized method for removing heavy metals by optoelectronic and microbial synergy according to claim 4, characterized in that, In the said Step 2, the pH of the buffer solution of MV is 4 - 6.

6. The compartmentalized method for removing heavy metals by using optoelectronic synergistic microorganisms as claimed in claim 1, wherein In the said Step 2, the vortex time is 1 - 12 h, the freezing temperature in the liquid nitrogen tank is -190 - -80 °C, and the freezing time is 2 - 8 h.

7. The compartmentalized method for removing heavy metals by using optoelectronic and microbial synergy according to claim 1, wherein In the said Step 2, the thawing temperature in the water bath is 50 - 70 °C, and the dialysis time is 50 - 120 h.

8. The compartmentalized method for removing heavy metals by optoelectronic synergistic microorganisms as claimed in claim 1, characterized in that, In the said Step 3, the concentration of wet cells of Alcaligenes faecalis is 1 - 5 g / L; the mass-volume ratio of titanium nitride semiconductor, wet cells of Alcaligenes faecalis to chloroform is 0.5 - 2 mg : 0.2 - 1.5 mg : 5 - 40 mL.

9. Application of a compartmentalized method for removing heavy metals by optoelectronic synergistic microorganisms according to any one of claims 1-8, characterized in that, Including: The above-described compartmentalization method for removing heavy metals by synergistic action of photoelectrons and microorganisms is applied to the reduction and removal of uranium in wastewater.

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