An insecticide containing thiazovypyr and pymetrozine and its application

Abstract

The present invention relates to the technical field of pesticides, and in particular to an insecticide comprising methoxazole and pyrimidine and an application thereof. The mass ratio of methoxazole to pyrimidine is 2:8-7:3. The insecticide provided by the present invention has an obvious synergistic effect on cabbage aphids and diamondback moths, can reduce the amount of medicaments used, reduce costs, reduce harm to the environment, and can effectively delay the generation of pest resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to an insecticide containing cyazofamid and pymetrozine and its application. Background Art

[0002] Cabbage is one of the important vegetables in China. The main pests that harm cabbage are aphids and diamondback moths, which have seriously affected the yield and quality of cabbage.

[0003] Aphids, also known as honeydew insects and greasy insects, belong to the order Homoptera and the family Aphididae. They mainly harm cruciferous vegetables such as cabbage, broccoli, cabbage, cauliflower, radish, Chinese cabbage, and turnip. Aphids can suck the sap of plants, causing poor growth of plants, curling and deformation of leaves, affecting heading, and easily causing sooty mold, and even spreading virus diseases. In the northern region, it occurs 8 - 20 or more generations a year, overwintering as eggs in the depressions of the rhizomes near the ground of the plants, at the base of the petioles, and on the leaves.

[0004] The diamondback moth, also known as the small green worm, belongs to the order Lepidoptera and the family Plutellidae. It is a pest that can cause devastating damage to cruciferous vegetables, especially crops such as cabbage, Chinese cabbage, cauliflower, rape, and radish are most severely affected. The diamondback moth is internationally recognized as one of the most serious vegetable pests with drug resistance. Due to its short generation cycle, strong reproductive ability, and easy generation of drug resistance, if it breaks out in large numbers, it will cause serious damage to the yield and quality of vegetables. In addition, the peak period of the diamondback moth outbreak is from March to April and from October to December every year.

[0005] However, currently, the insecticides for controlling cabbage aphids and diamondback moths have unsatisfactory control effects due to resistance problems, resulting in increased control efforts.

[0006] Therefore, it is necessary to develop an insecticide that can improve the control effect on cabbage aphids and diamondback moths and reduce drug resistance, which has certain social benefits and application values. There is no relevant report on the compound use of cyazofamid and pymetrozine in the prior art. Summary of the Invention

[0007] The purpose of the present invention is to provide an agricultural compound insecticide. In the provided agricultural compound insecticide, there is an insecticidal composition with obvious synergistic effects, which has the advantages of less dosage, low cost, good drug effect, can delay the drug resistance of pests, and is environmentally friendly.

[0008] The specific technical solution is as follows:

[0009] The present invention provides an insecticide, and the active ingredients of the insecticide comprise cyazofamid and pymetrozine guanidine. The mass ratio of cyazofamid to pymetrozine guanidine is 2:8 - 7:3; preferably, the mass ratio of cyazofamid to pymetrozine guanidine is 2:8 - 5:5; preferably, the mass ratio of cyazofamid to pymetrozine guanidine is 4:6 - 7:3; preferably, the mass ratio of cyazofamid to pymetrozine guanidine is 4:6.

[0010] In the insecticide provided by the present invention, the sum of the masses of cyazofamid and pymetrozine guanidine accounts for 1% - 70% of the total mass of the insecticide.

[0011] The dosage form of the insecticide provided by the present invention is a suspension concentrate, microemulsion, aqueous emulsion, wettable powder, water dispersible granule, dispersible oil suspension, granule.

[0012] The insecticide provided by the present invention further contains excipients, and the excipients are synergists, wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, carriers, fillers, etc., which can be appropriately added according to needs and are not particularly limited.

[0013] The synergist is PXG22. PXG22 is a diphenylpyrazole compound developed by Northwest A&F University and belongs to a new inhibitor of GSTs. It has no insecticidal activity by itself, but can reduce the activity of detoxifying enzymes in pests.

[0014] The insecticide provided by the present invention is a 30% suspension concentrate of cyazofamid and pymetrozine guanidine. By mass percentage, it contains 12% of cyazofamid, 18% of pymetrozine guanidine, and also includes a synergist, a dispersant, a wetting agent, a defoaming agent, a thickener, a preservative and deionized water.

[0015] The present invention provides a preparation method of the above insecticide. Deionized water, a dispersant and a wetting agent are mixed evenly, and then a defoaming agent and a preservative are added under stirring, and they are mixed evenly by high-speed shearing. Then the technical cyazofamid, the technical pymetrozine guanidine and a synergist are added, and shearing and mixing are continued. Then it enters a horizontal sand mill for primary sanding and secondary sanding, so that the particle size of the material is all below 5 μm. Then a thickener is added and sheared evenly to obtain the suspension concentrate of the present invention.

[0016] The insecticide provided by the present invention is used for the control of agricultural pests, and preferably for the control of cabbage aphids and diamondback moths.

[0017] Beneficial technical effects:

[0018] 1. The two active ingredients in the insecticide provided by the present invention are compounded in a certain proportion, which has obvious synergistic effects on cabbage aphids and diamondback moths, improves the control effect, expands the control spectrum, reduces the dosage of pesticides, reduces the use cost, and reduces the environmental pollution.

[0019] 2. The insecticide provided by the present invention, by adding PXG22 and combining the synergistic effect of flubendiamide and pymetrozine, can not only improve the control effect of the insecticide, but also delay or reduce drug resistance, extend the service life of the insecticide, and is of great significance for the management of pest drug resistance. Detailed implementation manners

[0020] The following further explains and illustrates the present invention in combination with specific implementation manners.

[0021] Example 1 Indoor toxicity determination of agents against cabbage aphids

[0022] Test pests: Select female adult cabbage aphids with uniform growth and development reared in a greenhouse for standby.

[0023] Test agents: Flubendiamide technical and pymetrozine technical.

[0024] Test method: The spraying method is adopted. Dissolve the flubendiamide technical and pymetrozine technical with acetone, dilute with 0.1% Tween-80 aqueous solution to prepare single-agent mother liquors, and prepare mother liquors of agent combinations with different ratios of flubendiamide and pymetrozine. Dilute the single agents and each combination into 5 concentration gradients with 0.1% Tween-80 aqueous solution to measure LC 50 . Use a writing brush to transfer female adult cabbage aphids with consistent growth onto the culture dishes containing cabbage leaves. Then place the culture dishes on the bottom plate of a Potter spray tower for quantitative spraying. Put 30 aphids in each dish, with 4 dishes for each treatment. Spray each dish evenly, and set a treatment without agent as a blank control. The tested insects after treatment are placed in an incubator at a temperature of 25°C, a relative humidity of 60%-80%, and a 16h / 8h light cycle for cultivation. Check the dead insects 48 hours after treatment, record the total number of insects and the number of dead insects. Calculate the mortality rate of each treatment based on the survey data. Conduct a regression analysis on the logarithm of each agent concentration and the corresponding mortality probability value to calculate the LC 50 of each agent treatment. Evaluate the interaction of the mixed agents according to the Wadley method. Among them, if SR = 0.5 - 1.5, then the two agents in the mixture have an additive effect; if SR ≤ 0.5, it is an antagonistic effect; if SR ≥ 1.5, it is a synergistic effect.

[0025] LC 50 (theoretical value) = (a + b) / (a / LC 50 a + b / LC 50 b)

[0026] SR = LC 50 (theoretical value) / LC 50 (actual value)

[0027] The test results are shown in Table 1.

[0028] Table 1 Results of the combined action of the compound agents against cabbage aphids

[0029]

[0030] As can be seen from Table 1, after the compounding of cyazofamid and pymetrozine according to the test ratio, there are two different combined actions of addition and synergism against cabbage aphids. When the mass ratio of cyazofamid to pymetrozine is 1:9, 6:4 - 9:1, the compound agent shows an additive effect on cabbage aphids. When the mass ratio of cyazofamid to pymetrozine is 2:8 - 5:5, it shows a synergistic effect on cabbage aphids. Especially when the mass ratio of cyazofamid to pymetrozine is 4:6, the SR value is 2.86, showing a significant synergistic effect.

[0031] Indoor toxicity determination of the agent in Example 2 against Plutella xylostella on cabbage

[0032] Control object: Select Plutella xylostella on cabbage that has been continuously reared indoors and has uniform growth.

[0033] Test method: The leaf dipping method is used. The original drugs of cyazofamid and pymetrozine are dissolved with acetone and diluted with a 0.1% Tween - 80 aqueous solution to prepare the mother liquor of single agents. The agent combinations with different ratios of cyazofamid and pymetrozine are prepared. The single agents and each combination are respectively diluted with a 0.1% Tween - 80 aqueous solution into 5 concentration gradients to measure LC 50 . The cabbage leaf discs are dipped in the liquid medicine for 10 s, taken out and dried, and placed in a petri dish on filter paper. The test insects are introduced, with 10 insects in each repetition and 4 repetitions for each treatment. A treatment without the agent is set as a blank control. The treated test insects are placed upside down and reared and observed under the conditions of a temperature of 25 °C, a relative humidity of 60% - 80%, and a photoperiod of 16 h / 8 h. The death situation of the test insects is checked 48 h after the treatment, and the total number of insects and the number of dead insects are recorded respectively. According to the survey data, the mortality rate of each treatment is calculated. According to the logarithmic values of the concentrations of each agent and the corresponding probit values of the mortality rate, a regression analysis is carried out to calculate the LC 50 . According to the Wadley method, the interaction of the mixed agents is evaluated. Among them, if SR = 0.5 - 1.5, the compounding of the two agents has an additive effect; if SR ≤ 0.5, it is an antagonistic effect; if SR ≥ 1.5, it is a synergistic effect.

[0034] LC 50 (Theoretical value)=(a + b) / (a / LC 50 a + b / LC 50 b)

[0035] SR = LC 50 (Theoretical value) / LC 50 (Actual value)

[0036] The test results are shown in Table 2.

[0037] Table 2 Results of the combined effect of the compound agents on Plutella xylostella in cabbage

[0038]

[0039] As can be seen from Table 2, the compound of flubendiamide and pymetrozine shows two different combined effects on Plutella xylostella, namely additive and synergistic effects. When the mass ratio of flubendiamide to pymetrozine is 1:9 - 3:7, 8:2 - 9:1, the compound agent shows an additive effect on Plutella xylostella in cabbage. When the mass ratio of flubendiamide to pymetrozine is 4:6 - 7:3, the compound agent shows a synergistic effect on Plutella xylostella in cabbage. Especially when the mass ratio of flubendiamide to pymetrozine is 4:6 and 5:5, the SR values are 2.28 and 2.45 respectively, showing a significant synergistic effect.

[0040] Based on Table 1 and Table 2 comprehensively, when the mass ratio of flubendiamide to pymetrozine is 4:6, the compound agent shows a significant synergistic effect on both cabbage aphids and Plutella xylostella simultaneously.

[0041] Example 3 Preparation and quality performance of 30% flubendiamide·pymetrozine suspension concentrate

[0042] The preparation formula of 30% flubendiamide·pymetrozine suspension concentrate is shown in Table 3.

[0043] Table 3 Preparation formula of 30% flubendiamide·pymetrozine suspension concentrate

[0044] Serial number Formulation components Ratio 1 Tolfenpyrad technical 12.0％ 2 Metaflumizone technical 18.0％ 3 PXG22 15％ 4 Comb-shaped graft copolymer dispersant (Atlox4913) 6.0％ 5 Alcohol ethoxylate wetting agent (Lutensol·T08) 4.0％ 6 Silicone defoamer (SAG1572) 0.1％ 7 BIT preservative (G20S) 0.3％ 8 Magnesium aluminum silicate thickener (SF-04) 1.0％ 9 Xanthan gum thickener 0.13％ 10 Deionized water Make up to 100%

[0045] Preparation of 30% flubendiamide·pymetrozine suspension concentrate: According to the formula in Table 3 above, deionized water, dispersant, and wetting agent are mixed evenly. Under stirring, defoamer and preservative are added, and after being mixed evenly by high-speed shearing, flubendiamide technical, pymetrozine technical, and synergist are added, and continue to shear and mix evenly. Then it enters the horizontal sand mill for primary and secondary sand grinding to make the particle size of all materials below 5μm, and then thickener is added and sheared evenly to obtain the product.

[0046] Quality performance detection: It is measured according to the enterprise standard regulations of this product, and the detection results are shown in Table 4.

[0047] Table 4 Quality detection results of 30% flubendiamide·pymetrozine suspension concentrate

[0048]

[0049] It can be seen from Table 4 that all the quality detection results of 30% flubendiamide·pymetrozine suspension concentrate meet the requirements of the enterprise standard indicators.

[0050] Field control efficacy of the compound agent against cabbage aphids

[0051] Test method: Apply the medicine when the cabbage aphids are in the initial peak stage. The test adopts a randomized block arrangement, with protective rows set around. Each treatment has 4 replicates, and a clear water control is set. Each plot is 30m 2 . Investigate the initial population of insects before spraying. The five-point sampling method is adopted. At each sampling point, 4 cabbage plants are investigated, and fixed-point and fixed-plant investigations are implemented. Use a knapsack electric sprayer to spray evenly. For each medicine treatment, spray each plot evenly according to the water consumption of 30kg per mu. No other medicines are used during the test period. Investigate the number of insects on the 1st, 7th, and 14th days after spraying respectively, calculate the reduction rate of the insect population and the corrected control efficacy. The results are shown in Table 5.

[0052] Reduction rate of insect population (%) = (Initial population of insects before treatment - Surviving insects after treatment)

[0053] ×100 / Initial population of insects before treatment;

[0054] Corrected control efficacy (%) = (Reduction rate of insect population in the treatment area - Reduction rate of insect population in the control area) × 100 / (100 - Reduction rate of insect population in the control area).

[0055] Table 5 Determination of field control efficacy of the compound agent against cabbage aphids

[0056]

[0057] It can be seen from Table 5 that the field control efficacy of the medicine against cabbage aphids increases with the increase of the dose. Under the same application dose, the control efficacy of the mixture against cabbage aphids is significantly better than that of the single agent, and both the quick-acting property and the long-lasting property are excellent. Through field observation, the treatment medicine is safe for cabbage plants.

[0058] Example 5 Field control efficacy of the compound agent against diamondback moth on cabbage

[0059] Test method: Apply the medicine when the diamondback moth on cabbage is in the initial peak stage of low-instar larvae. The test adopts a randomized block arrangement, with protective rows set around. Each treatment has 4 replicates, and a clear water control is set. Each plot is 30m 2 . Investigate the initial population of insects before spraying. The five-point sampling method is adopted. At each sampling point, 4 cabbage plants are investigated, and fixed-point and fixed-plant investigations are implemented. Use a knapsack electric sprayer to spray evenly. For each medicine treatment, spray each plot evenly according to the water consumption of 30kg per mu. No other medicines are used during the test period. Investigate the number of insects on the 1st, 7th, and 14th days after spraying respectively, calculate the reduction rate of the insect population and the corrected control efficacy. The results are shown in Table 6.

[0060] Reduction rate of insect population (%) = (Initial population of insects before treatment - Surviving insects after treatment) × 100 / Initial population of insects before treatment;

[0061] Correction control efficacy (%) = (Insect population reduction rate in treatment area - Insect population reduction rate in control area) × 100 / (100 - Insect population reduction rate in control area).

[0062] Table 6 Determination of field control efficacy of compound agents against Plutella xylostella on cabbage

[0063]

[0064] As can be seen from Table 6, the field control efficacy of the agents against Plutella xylostella on cabbage increases with the increase of dosage. Under the same application dosage, the control efficacy of the mixture against Plutella xylostella is significantly better than that of the single agent, and both the quick-acting property and the long-lasting property are excellent. Through field observation, the treated agents are safe for cabbage plants.

[0065] Based on Table 5 and Table 6, it can be seen that the compound agents of cyantraniliprole and vilantropis have good control efficacy against cabbage aphids and Plutella xylostella, and the control efficacy is significantly higher than that of the single agent, and both the quick-acting property and the long-lasting property are excellent. The compound agents of cyantraniliprole and vilantropis improve the control effect, expand the control spectrum, reduce the dosage of pesticides, and delay the generation of pest resistance.

[0066] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them.

Claims

1. An insecticide, characterized in that, The active ingredients of the insecticide comprise cyazofamid and pymetrozine-guanidine, and the mass ratio of cyazofamid to pymetrozine-guanidine is 4:6 - 5:

5.

2. The insecticide according to claim 1, wherein, The percentage content of the sum of the masses of cyazofamid and pymetrozine-guanidine in the total mass of the insecticide is 1% - 70%.

3. The insecticide according to claim 1, characterized in that, The dosage form is suspension concentrate, microemulsion, emulsion in water, wettable powder, water dispersible granule, dispersible oil suspension, granule.

4. The pesticide according to claim 1, wherein The auxiliary materials are synergist, wetting agent, dispersant, emulsifier, thickener, disintegrant, antifreezing agent, defoaming agent, solvent, preservative, carrier, filler.

5. The pesticide according to claim 4, characterized in that, The synergist is PXG22.

6. The pesticide according to claim 1, characterized in that, The insecticide is 30% cyazofamid and pymetrozine-guanidine suspension concentrate.

7. The insecticide according to claim 6, wherein The formulation is, by mass percentage, 12% cyazofamid, 18% pymetrozine-guanidine, and also includes synergist, dispersant, wetting agent, defoaming agent, thickener, preservative and deionized water.

8. The insecticide according to claim 6, wherein The preparation method is to mix deionized water, dispersant and wetting agent evenly, then add defoaming agent and preservative under stirring, mix evenly by high-speed shearing, then add cyazofamid technical, pymetrozine-guanidine technical and synergist, continue to shear and mix evenly, then enter a horizontal sand mill for grinding so that all the particle sizes of the materials are below 5 μm, and then add thickener and shear evenly to obtain the suspension concentrate.

9. The pesticide according to any one of claims 1-8, characterized in that, It is used for controlling cabbage aphids and diamondback moths.

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