A bactericidal composition containing menthone

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing menthone. A bactericidal composition containing menthone, the active ingredients of which are compounded by magnolol, carvacrol or oregano oil and menthone. The present invention compounded magnolol, carvacrol or oregano oil and menthone in a certain mass, showing a synergistic effect on a variety of plant pathogenic fungi, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and is of great significance for the high-quality and high-yield of crops such as wheat, peanut and maize.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing menthone. Background Art

[0002] The occurrence of diseases poses a major threat to agricultural production. The use of chemical pesticides plays an important role in preventing and controlling crop diseases and ensuring stable and high yields of agricultural and forestry crops. However, the long-term application of chemical pesticides is likely to cause consequences such as human and livestock poisoning, high residues, and environmental pollution, thus bringing potential hazards to the ecological environment and human health.

[0003] Botanical fungicides refer to agents processed from certain parts of plants with bactericidal and antibacterial activities, or their active ingredients extracted, as well as monomer substances separated and purified for controlling plant diseases. Botanical fungicides have gradually become a hot topic in plant disease control due to their unique advantages such as high efficiency, low toxicity, low residues, high selectivity, and little impact on the environment. At present, domestic and foreign scholars have conducted a large number of studies on the control of fungal diseases by botanical fungicides and found many plant resources with bactericidal effects, which provides an important basis for the research on disease prevention and control using botanical fungicides.

[0004] Currently, there are many botanical fungicides on the market, including ethyllicin, kasugamycin, eugenol, carvacrol, osthole, etc., and they have been popularized and applied in the control of various crop diseases, achieving remarkable control effects. For example, Wang Zhifeng et al. reported that eugenol has strong antifungal effects, and the control effect on tomato late blight can reach 78%; Peng Zhiguo et al. reported that the control effect of 1% osthole on cucumber powdery mildew in greenhouse reached 79.33% in greenhouse; Sun Xuemei et al. used ethyllicin, kasugamycin, ningnanmycin and zhongshengmycin by root dipping treatment, and the control effects on strawberry leaf spot disease could all reach over 80%; at the same time, the latest research by Zheng Anke et al. showed that 9 tested botanical fungicides such as eugenol, allicin and osthole have inhibitory effects on Puccinia helianthi Schw., and 0.3% eugenol has the strongest toxicity, and the inhibitory effect is as high as over 85%, further indicating that botanical fungicides have strong potential for controlling plant diseases.

[0005] Menthone belongs to monoterpenoid compounds and is widely present in the volatile oils of many plants such as mint, schizonepeta, scutellaria and pogostemon cablin. And studies have shown that it has antiviral, anti-inflammatory, cholagogic and permeability-promoting effects. Currently, menthone and its derivatives are widely used in medicine, mainly as local anesthetics and protective agents, etc., but there are few reports in the research process of plant diseases.

[0006] In previous research, the inventors found that menthone has good antibacterial effects against wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab, etc., showing good potential for disease prevention and control. To further use menthone for plant disease prevention and control, the inventors carried out a compounding study of menthone with plant-derived fungicides such as magnolol, carvacrol, oregano oil, osthole, eugenol, resveratrol, and allicin. Some of the compounding combinations showed strong synergistic effects. The research results provided data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab, and were of great significance for the high-quality and high-yield of crops such as wheat, peanuts, and maize.

[0007] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0008] The object of the present invention is to provide a bactericidal composition containing menthone, which can effectively inhibit the growth of pathogenic bacteria such as wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab, effectively prevent and control various plant fungal diseases, and is of great significance for the high-quality and high-yield of crops such as wheat, peanuts, and maize.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A bactericidal composition containing menthone, the active ingredient of which is compounded by magnolol, carvacrol or oregano oil and menthone.

[0011] Preferably, the mass ratio of magnolol to menthone is 1 - 9:9 - 1.

[0012] Preferably, the mass ratio of carvacrol to menthone is 1 - 9:9 - 1.

[0013] Preferably, the mass ratio of oregano oil to menthone is 1 - 9:9 - 1.

[0014] The present invention also provides the use of the bactericidal composition containing menthone in the prevention and control of fungal diseases of crops.

[0015] Preferably, the fungal diseases of crops include wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention combines magnolol, carvacrol or origanum oil with menthone in a certain mass, showing a synergistic effect on various pathogenic bacteria of plant fungal diseases, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and having important significance for the high-quality and high-yield of crops such as wheat, peanut and maize. Detailed implementation mode

[0018] The following clearly and completely describes the technical solution of the present invention patent. Example

[0019] 1. Test strains

[0020] Table 1 Test strains

[0021] Chinese name of the strain Latin name of the strain Disease name Strain source Sclerotium rolfsii Sacc Peanut southern blight Zhengzhou City, Henan Province Fusarium pseudograminearum Wheat basal rot Xinxiang City, Henan Province Rhizoctonia solani Wheat sheath blight Xinxiang City, Henan Province Fusarium graminearum Wheat head blight / Corn head blight Xinxiang City, Henan Province Fusarium incarnatum Peanut root rot Zhengzhou City, Henan Province Fusarium equiseti Peanut root rot Zhengzhou City, Henan Province Fusarium proliferatum Peanut root rot Zhengzhou City, Henan Province Fusarium oxysporum Peanut root rot Xinxiang City, Henan Province

[0022] 2. Test agents

[0023] 98% magnolol technical (Shanghai Yuanye Bio-Technology Co., Ltd.), 92.5% carvacrol technical (Shanghai Yuanye Bio-Technology Co., Ltd.), 98% origanum oil technical (Hubei Hengjingrui Chemical Co., Ltd.), 99% menthone technical (Wuhan Rongcan Biotechnology Co., Ltd.).

[0024] After the test agents are dissolved, they are diluted into single-agent mother liquors with a 0.1% Tween-80 aqueous solution, and multiple groups of ratios are set. Each single-agent mother liquor and the ratio mixture are set with 5 mass concentration gradients according to the equal ratio method.

[0025] 3. Test method

[0026] Add 9 mL of pre-melted PDA medium into a sterile conical flask. Quantitatively absorb 1 mL of the liquid medicine from low concentration to high concentration in turn and add it into the above conical flask. After shaking well, pour it into a petri dish with a diameter of 9 cm to make a drug-containing plate with the corresponding concentration. At the same time, set a treatment without the agent as a blank control, and treat 10 plates with each mass concentration of the liquid medicine. Use a punch with a diameter of 5 mm to cut out a fungal cake at the edge of the test strain colony and inoculate it in the center of the drug-containing plate and the blank control plate. Cover the petri dish lid and place it in an incubator at 25 °C for cultivation. When the diameter of the blank control colony grows to about 2 / 3 of the diameter of the petri dish, measure the colony diameter by the cross method and calculate the inhibition rate of mycelial growth for different treatments.

[0027]

[0028] 4. Data analysis

[0029] Use DPS software for data statistical analysis to obtain the virulence regression equation and the virulence EC of the agent against the target pathogen 50Values were obtained and the co-toxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

[0030]

[0031] In the above formula: ATI - The measured toxicity index of the mixture; S - The EC 50 of the standard agent, with the unit of mg / L; M - The EC 50 of the mixture, with the unit of mg / L.

[0032]

[0033] In the above formula: TTI - The theoretical toxicological index of the mixture; TI A - The toxicity index of agent A; P A - The percentage content of agent A in the mixture, with the unit of percentage (%); TI B - The toxicity index of agent B; P B - The percentage content of agent B in the mixture, with the unit of percentage (%).

[0034]

[0035] In the above formula: CTC - The co-toxicity coefficient; ATI - The measured toxicity index of the mixture; TTI - The theoretical toxicity index of the mixture.

[0036] 5. Determination Results

[0037] The synergistic effect of the agent was evaluated according to the calculated co-toxicity coefficient (CTC). When CTC ≤ 80, it was an antagonistic effect; when 80 < CTC < 120, it was an additive effect; when CTC ≥ 120, it was a synergistic effect. The results are shown in Table 2-25.

[0038] Table 2 Indoor Biological Activity Determination of the Compound of Magnolol and Mentholone against Sclerotium rolfsii

[0039] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 16.1248 100.0000 -- -- Menthone 59.6386 27.0375 -- -- Magnolol 9:Menthone 1 4.8374 333.3361 92.7038 359.5713 Magnolol 8:Menthone 2 2.6875 599.9926 85.4075 702.5057 Magnolol 7:Menthone 3 2.5460 633.3386 78.1113 810.8160 Magnolol 6:Menthone 4 5.3749 300.0019 70.8150 423.6416 Magnolol 5:Menthone 5 0.5691 2833.3860 63.5188 4460.7073 Magnolol 4:Menthone 6 8.0624 200.0000 56.2225 355.7294 Magnolol 3:Menthone 7 1.1799 1366.6243 48.9263 2793.2324 Magnolol 2:Menthone 8 3.0234 533.3333 41.6300 1281.1268 Magnolol 1:Menthone 9 4.0312 400.0000 34.3338 1165.0337

[0040] As can be seen from Table 2, the compound of magnolol and mentholone showed excellent antibacterial activity against Sclerotium rolfsii. The co-toxicity coefficients of all tested compound combinations against Sclerotium rolfsii were greater than 120, showing a synergistic effect. Especially when the compound ratio of magnolol and mentholone was 5:5, the co-toxicity coefficient reached 4460.7073, and the synergistic effect was significant.

[0041] Table 3 Indoor bioactivity determination of magnolol and menthone complex against Fusarium pseudograminearum

[0042] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 4.5289 100.0000 -- -- Menthone 80.5613 5.6217 -- -- Magnolol 9:Menthone 1 5.3620 84.4629 90.5622 93.2651 Magnolol 8:Menthone 2 5.1518 87.9091 81.1243 108.3634 Magnolol 7:Menthone 3 10.5096 43.0930 71.6865 60.1131 Magnolol 6:Menthone 4 9.7288 46.5515 62.2487 74.7831 Magnolol 5:Menthone 5 9.8311 46.0671 52.8108 87.2303 Magnolol 4:Menthone 6 5.4737 82.7393 43.3730 190.7621 Magnolol 3:Menthone 7 5.5902 81.0150 33.9352 238.7345 Magnolol 2:Menthone 8 6.2557 72.3964 24.4973 295.5274 Magnolol 1:Menthone 9 7.7276 58.6068 15.0595 389.1680

[0043] As can be seen from Table 3, the complex of magnolol and menthone showed excellent antibacterial activity against Fusarium pseudograminearum. When the compounding ratio of magnolol and menthone was 7:3 and 6:4, it showed antagonism; when the compounding ratio of magnolol and menthone was 9:1, 8:2 and 5:5, it showed addition; the co-toxicity coefficients of the other compounding combinations against Fusarium pseudograminearum were all greater than 120, showing a synergistic effect.

[0044] Table 4 Indoor bioactivity determination of magnolol and menthone complex against Rhizoctonia solani

[0045] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 4.7179 100.0000 -- -- Menthone 30.6867 15.3744 -- -- Magnolol 9:Menthone 1 3.7247 126.6652 91.5374 138.3753 Magnolol 8:Menthone 2 4.0439 116.6671 83.0749 140.4360 Magnolol 7:Menthone 3 3.3700 139.9970 74.6123 187.6326 Magnolol 6:Menthone 4 2.1944 214.9973 66.1498 325.0159 Magnolol 5:Menthone 5 2.1284 221.6642 57.6872 384.2519 Magnolol 4:Menthone 6 9.4359 49.9995 49.2246 101.5741 Magnolol 3:Menthone 7 14.1538 33.3331 40.7621 81.7748 Magnolol 2:Menthone 8 28.3076 16.6665 32.2995 51.6000 Magnolol 1:Menthone 9 47.1793 9.9999 23.8370 41.9514

[0046] As can be seen from Table 4, the complex of magnolol and menthone showed excellent antibacterial activity against Rhizoctonia solani. When the compounding ratio of magnolol and menthone was 2:8 and 1:9, it showed antagonism; when the compounding ratio of magnolol and menthone was 4:6 and 3:7, it showed addition; the co-toxicity coefficients of the other compounding combinations against Rhizoctonia solani were all greater than 120, showing a synergistic effect.

[0047] Table 5 Indoor bioactivity determination of magnolol and menthone complex against Fusarium graminearum

[0048] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 3.0035 100.0000 -- -- Menthone 58.2502 5.1562 -- -- Magnolol 9:Menthone 1 1.3564 221.4317 90.5156 244.6337 Magnolol 8:Menthone 2 1.2742 235.7165 81.0312 290.8959 Magnolol 7:Menthone 3 0.8761 342.8262 71.5469 479.1631 Magnolol 6:Menthone 4 0.9826 305.6686 62.0625 492.5176 Magnolol 5:Menthone 5 0.8410 357.1344 52.5781 679.2454 Magnolol 4:Menthone 6 0.7509 399.9867 43.0937 928.1785 Magnolol 3:Menthone 7 0.7645 392.8712 33.6093 1168.9343 Magnolol 2:Menthone 8 0.6893 435.7319 24.1250 1806.1453 Magnolol 1:Menthone 9 0.5760 521.4410 14.6406 3561.6130

[0049] As can be seen from Table 5, the complex of magnolol and menthone showed excellent antibacterial activity against Fusarium graminearum. The co-toxicity coefficients of all tested compounding combinations against Fusarium graminearum were all greater than 120, showing a synergistic effect. Especially when the compounding ratio of magnolol and menthone was 1:9, the co-toxicity coefficient reached 3561.6130, showing a significant synergistic effect.

[0050] Table 6 Indoor bioactivity determination of magnolol and menthone complex against Fusarium incarnatum

[0051] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 3.3194 100.0000 -- -- Menthone 110.2090 3.0119 -- -- Magnolol 9:Menthone 1 4.0854 81.2503 90.3012 89.9770 Magnolol 8:Menthone 2 4.5201 73.4364 80.6024 91.1095 Magnolol 7:Menthone 3 5.9012 56.2496 70.9036 79.3325 Magnolol 6:Menthone 4 5.1815 64.0625 61.2048 104.6692 Magnolol 5:Menthone 5 6.6388 50.0000 51.5060 97.0762 Magnolol 4:Menthone 6 13.2777 24.9998 41.8071 59.7979 Magnolol 3:Menthone 7 9.8163 33.8152 32.1083 105.3159 Magnolol 2:Menthone 8 12.4966 26.5624 22.4095 118.5318 Magnolol 1:Menthone 9 7.1163 46.6450 12.7107 366.9738

[0052] As can be seen from Table 6, the complex of magnolol and menthone showed excellent antibacterial activity against Fusarium incarnatum. When the compounding ratio of magnolol and menthone was 1:9, the co-toxicity coefficients of the compounding combinations against Fusarium incarnatum were all greater than 120, showing a synergistic effect.

[0053] Table 7 Indoor bioactivity determination of magnolol and menthone complex against Fusarium equiseti

[0054] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 4.1399 100.0000 -- -- Menthone 145.5956 2.8434 -- -- Magnolol 9:Menthone 1 4.5681 90.6263 90.2843 100.3788 Magnolol 8:Menthone 2 8.2797 50.0006 80.5687 62.0596 Magnolol 7:Menthone 3 9.4626 43.7501 70.8530 61.7477 Magnolol 6:Menthone 4 6.9724 59.3755 61.1374 97.1182 Magnolol 5:Menthone 5 8.1354 50.8875 51.4217 98.9611 Magnolol 4:Menthone 6 13.2476 31.2502 41.7061 74.9296 Magnolol 3:Menthone 7 8.6565 47.8242 31.9904 149.4954 Magnolol 2:Menthone 8 12.4825 33.1656 22.2747 148.8935 Magnolol 1:Menthone 9 10.1905 40.6251 12.5591 323.4718

[0055] As can be seen from Table 7, the combination of magnolol and menthone exhibits excellent antibacterial activity against Fusarium equiseti. When the compounding ratio of magnolol to menthone is 3:7, 2:8, and 1:9, the co-toxicity coefficients of the compounding combinations against Fusarium equiseti are all greater than 120, showing a synergistic effect.

[0056] Table 8 Indoor bioactivity determination of the combination of magnolol and menthone against Fusarium proliferatum

[0057] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 6.2200 100.0000 -- -- Menthone 114.0774 5.4524 -- -- Magnolol 9:Menthone 1 7.5069 82.8571 90.5452 91.5091 Magnolol 8:Menthone 2 8.4952 73.2178 81.0905 90.2915 Magnolol 7:Menthone 3 9.0708 68.5717 71.6357 95.7227 Magnolol 6:Menthone 4 7.2233 86.1102 62.1810 138.4832 Magnolol 5:Menthone 5 7.5069 82.8571 52.7262 157.1459 Magnolol 4:Menthone 6 8.7081 71.4278 43.2715 165.0690 Magnolol 3:Menthone 7 6.4029 97.1435 33.8167 287.2648 Magnolol 2:Menthone 8 7.2566 85.7151 24.3620 351.8399 Magnolol 1:Menthone 9 8.3731 74.2855 14.9072 498.3198

[0058] As can be seen from Table 8, the combination of magnolol and menthone exhibits excellent antibacterial activity against Fusarium proliferatum. When the compounding ratio of magnolol to menthone is 9:1, 8:2, and 7:3, the compounding combinations show an additive effect against Fusarium proliferatum; the other compounding combinations show a synergistic effect against Fusarium proliferatum.

[0059] Table 9 Indoor bioactivity determination of the combination of magnolol and menthone against Fusarium oxysporum

[0060] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 6.2780 100.0000 -- -- Menthone 144.7977 4.3357 -- -- Magnolol 9:Menthone 1 5.4933 114.2847 90.4336 126.3742 Magnolol 8:Menthone 2 6.2321 100.7365 80.8671 124.5704 Magnolol 7:Menthone 3 4.9389 127.1133 71.3007 178.2778 Magnolol 6:Menthone 4 6.5912 95.2482 61.7343 154.2874 Magnolol 5:Menthone 5 7.9902 78.5712 52.1679 150.6124 Magnolol 4:Menthone 6 8.2399 76.1902 42.6014 178.8444 Magnolol 3:Menthone 7 6.9389 90.4754 33.0350 273.8776 Magnolol 2:Menthone 8 5.8595 107.1422 23.4686 456.5352 Magnolol 1: Menthol 9 7.8813 79.6569 13.9021 572.9833

[0061] As can be seen from Table 9, the combination of magnolol and menthone exhibits excellent antibacterial activity against Fusarium oxysporum. The co-toxicity coefficients of all the tested compounding combinations against Fusarium oxysporum are greater than 120, showing a synergistic effect. When the compounding ratio of magnolol to menthone is 1:9, the co-toxicity coefficient reaches 572.9833, and the synergistic effect is significant.

[0062] Table 10 Indoor bioactivity determination of the combination of carvacrol and menthone against Sclerotium rolfsii

[0063] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 48.2768 100.0000 -- -- Menthol 59.6386 80.9489 -- -- Carvacrol 9: Menthol 1 59.1144 81.6667 98.0949 83.2528 Carvacrol 8: Menthol 2 53.6408 90.0001 96.1898 93.5652 Carvacrol 7: Menthol 3 27.8520 173.3333 94.2847 183.8404 Carvacrol 6: Menthol 4 28.9661 166.6666 92.3796 180.4150 Carvacrol 5: Menthol 5 29.9661 161.1047 90.4745 178.0665 Carvacrol 4: Menthol 6 68.9668 70.0001 88.5693 79.0342 Carvacrol 3: Menthol 7 181.0379 26.6667 86.6642 30.7701 Carvacrol 2: Menthol 8 1448.3028 3.3333 84.7591 3.9327 Carvacrol 1: Menthol 9 111.4079 43.3334 82.8540 52.3009

[0064] As can be seen from Table 10, the combination of carvacrol and menthone exhibits excellent antibacterial activity against Sclerotium rolfsii. When the compounding ratio of carvacrol to menthone is 7:3, 6:4, and 5:5, the co-toxicity coefficients of the compounding combinations against Sclerotium rolfsii are all greater than 120, showing a synergistic effect.

[0065] Table 11 Indoor bioactivity determination of the combination of carvacrol and menthone against Pseudograminearum

[0066] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 17.5498 100.0000 -- -- Menthol 80.5613 21.7844 -- -- Carvacrol 9: Menthol 1 17.0882 102.7013 92.1784 111.4157 Carvacrol 8: Menthol 2 23.1911 75.6747 84.3569 89.7078 Carvacrol 7: Menthol 3 19.6773 89.1880 76.5353 116.5319 Carvacrol 6: Menthol 4 23.6127 74.3236 68.7138 108.1640 Carvacrol 5: Menthol 5 24.5038 71.6207 60.8922 117.6189 Carvacrol 4: Menthol 6 22.0119 79.7287 53.0706 150.2313 Carvacrol 3: Menthol 7 24.0500 72.9721 45.2491 161.2677 Carvacrol 2: Menthol 8 36.0750 48.6481 37.4275 129.9795 Carvacrol 1: Menthol 9 24.975 70.2695 29.6060 237.3490

[0067] As can be seen from Table 11, the combination of carvacrol and menthone exhibits excellent antibacterial activity against Pseudograminearum. When the compounding ratio of carvacrol to menthone is 9 - 5:1 - 5, it shows an additive effect; the co-toxicity coefficients of the other compounding combinations against Pseudograminearum are all greater than 120, showing a synergistic effect.

[0068] Table 12 Indoor bioactivity determination of the combination of carvacrol and menthone against Rhizoctonia solani

[0069] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 11.0604 100.0000 -- -- Menthol 30.6867 36.0430 -- -- Carvacrol 9: Menthol 1 10.0421 110.1403 93.6043 117.6659 Carvacrol 8: Menthol 2 10.9836 100.6992 87.2086 115.4694 Carvacrol 7: Menthol 3 10.7321 103.0590 80.8129 127.5280 Carvacrol 6: Menthol 4 14.9564 73.9510 74.4172 99.3735 Carvacrol 5: Menthol 5 25.1054 44.0559 68.0215 64.7676 Carvacrol 4: Menthol 6 35.1475 31.4685 61.6258 51.0639 Carvacrol 3: Menthol 7 22.6758 48.7762 55.2301 88.3146 Carvacrol 2: Menthol 8 37.4340 29.5464 48.8344 60.5033 Carvacrol 1: Menthol 9 35.1475 31.4685 42.4387 74.1506

[0070] As can be seen from Table 12, the combination of carvacrol and menthone showed excellent antibacterial activity against Rhizoctonia solani. When the mixing ratio of carvacrol and menthone was 7:3, the co-toxicity coefficient of the combination against Rhizoctonia solani was greater than 120, showing a synergistic effect.

[0071] Table 13 Indoor bioactivity determination of the combination of carvacrol and menthone against Fusarium graminearum

[0072] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 24.9855 100.0000 -- -- Menthol 58.2502 42.8934 -- -- Carvacrol 9: Menthol 1 21.5260 116.0713 94.2893 123.1011 Carvacrol 8: Menthol 2 27.4351 91.0713 88.5787 102.8140 Carvacrol 7: Menthol 3 24.9855 100.0000 82.8680 120.6738 Carvacrol 6: Menthol 4 26.3998 94.6428 77.1574 122.6620 Carvacrol 5: Menthol 5 27.4351 91.0713 71.4467 127.4674 Carvacrol 4: Menthol 6 22.5675 110.7145 65.7360 168.4228 Carvacrol 3: Menthol 7 17.4899 142.8567 60.0254 237.9938 Carvacrol 2: Menthol 8 31.0931 80.3571 54.3147 147.9471 Carvacrol 1: Menthol 9 21.5260 116.0713 48.6041 238.8097

[0073] As can be seen from Table 13, the combination of carvacrol and menthone showed excellent antibacterial activity against Fusarium graminearum. When the mixing ratio of carvacrol and menthone was 8:2, the combination showed an additive effect against Fusarium graminearum, and the co-toxicity coefficients of the other combinations against Fusarium graminearum were all greater than 120, showing a synergistic effect.

[0074] Table 14 Indoor bioactivity determination of the combination of carvacrol and menthone against Fusarium incarnatum

[0075] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 27.2423 100.0000 -- -- Menthol 110.2090 24.7188 -- -- Carvacrol 9: Menthol 1 30.8098 88.4209 92.4719 95.6192 Carvacrol 8: Menthol 2 51.7604 52.6315 84.9438 61.9605 Carvacrol 7: Menthol 3 44.6211 61.0525 77.4156 78.8633 Carvacrol 6: Menthol 4 24.6478 110.5263 69.8875 158.1489 Carvacrol 5: Menthol 5 30.8098 88.4209 62.3594 141.7924 Carvacrol 4: Menthol 6 76.1183 35.7894 54.8313 65.2719 Carvacrol 3: Menthol 7 99.5393 27.3684 47.3031 57.8574 Carvacrol 2: Menthol 8 69.9465 38.9473 39.7750 97.9191 Carvacrol 1: Menthol 9 57.5116 47.3684 32.2469 146.8928

[0076] As can be seen from Table 14, the combination of carvacrol and menthone showed excellent antibacterial activity against Fusarium incarnatum. When the mixing ratios of carvacrol and menthone were 6:4, 5:5, and 1:9, the co-toxicity coefficients of the combinations against Fusarium incarnatum were all greater than 120, showing a synergistic effect.

[0077] Table 15 Indoor bioactivity determination of the combination of carvacrol and menthone against Fusarium equiseti

[0078] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 31.9803 100.0000 -- -- Menthol 145.5956 21.9652 -- -- Carvacrol 9: Menthol 1 31.9803 100.0000 92.1965 108.4640 Carvacrol 8: Menthol 2 36.1068 88.5714 84.3930 104.9511 Carvacrol 7: Menthol 3 37.9167 84.3436 76.5895 110.1241 Carvacrol 6: Menthol 4 44.7724 71.4286 68.7861 103.8417 Carvacrol 5: Menthol 5 46.8594 68.2474 60.9826 111.9129 Carvacrol 4: Menthol 6 41.4559 77.1429 53.1791 145.0625 Carvacrol 3: Menthol 7 37.3103 85.7144 45.3756 188.8997 Carvacrol 2: Menthol 8 65.8417 48.5715 37.5721 129.2754 Carvacrol 1: Menthol 9 36.1068 88.5714 29.7686 297.5326

[0079] As can be seen from Table 15, the combination of carvacrol and menthone showed excellent antibacterial activity against Fusarium equiseti. When the mixing ratios of carvacrol and menthone were 4 - 1:6 - 9, the co-toxicity coefficients of the combinations against Fusarium equiseti were all greater than 120, showing a synergistic effect.

[0080] Table 16 Indoor bioactivity determination of the combination of carvacrol and menthone against Fusarium proliferatum

[0081] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 29.8643 100.0000 -- -- Menthol 114.0774 26.1790 -- -- Carvacrol 9: Menthol 1 38.8236 76.9231 92.6179 83.0542 Carvacrol 8: Menthol 2 70.5883 42.3077 85.2358 49.6361 Carvacrol 7: Menthol 3 37.8767 78.8461 77.8537 101.2747 Carvacrol 6: Menthol 4 55.4623 53.8461 70.4716 76.4083 Carvacrol 5: Menthol 5 33.0414 90.3845 63.0895 143.2639 Carvacrol 4: Menthol 6 31.0589 96.1538 55.7074 172.6050 Carvacrol 3: Menthol 7 47.0678 63.4495 48.3253 131.2968 Carvacrol 2: Menthol 8 41.9714 71.1539 40.9432 173.7870 Carvacrol 1: Menthol 9 55.4623 53.8461 33.5611 160.4422

[0082] As can be seen from Table 16, the combination of carvacrol and menthone exhibits excellent antibacterial activity against Fusarium proliferatum. When the mixing ratio of carvacrol to menthone is 5-1:5-1, the combined effect on Fusarium proliferatum is additive; for the other combined mixtures, the effect is synergistic.

[0083] Table 17 Indoor bioactivity determination of the combination of carvacrol and menthone against Fusarium oxysporum

[0084] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 29.5089 100.0000 -- -- Menthol 144.7977 20.3794 -- -- Carvacrol 9: Menthol 1 41.4720 71.1538 92.0379 77.3092 Carvacrol 8: Menthol 2 43.8418 67.3077 84.0759 80.0559 Carvacrol 7: Menthol 3 49.4988 59.6154 76.1138 78.3240 Carvacrol 6: Menthol 4 80.7612 36.5385 68.1518 53.6134 Carvacrol 5: Menthol 5 85.2479 34.6154 60.1897 57.5105 Carvacrol 4: Menthol 6 49.4988 59.6154 52.2276 114.1453 Carvacrol 3: Menthol 7 54.8022 53.8462 44.2656 121.6435 Carvacrol 2: Menthol 8 76.7231 38.4616 36.3035 105.9444 Carvacrol 1: Menthol 9 42.1118 70.0728 28.3415 247.2447

[0085] As can be seen from Table 17, the combination of carvacrol and menthone exhibits excellent antibacterial activity against Fusarium oxysporum. When the mixing ratios of carvacrol to menthone are 3:7 and 1:9, the co-toxicity coefficients of the combined mixtures against Fusarium oxysporum are both greater than 120, showing a synergistic effect.

[0086] Table 18 Indoor bioactivity determination of the combination of oregano oil and menthone against Sclerotium rolfsii

[0087] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 52.2065 100.0000 -- -- Menthol 59.6386 87.5381 -- -- Origanum oil 9: Menthol 1 24.0030 217.4999 98.7538 220.2446 Origanum oil 8: Menthol 2 31.6403 165.0000 97.5076 169.2176 Origanum oil 7: Menthol 3 26.4336 197.5005 96.2614 205.1710 Origanum oil 6: Menthol 4 14.1099 369.9991 95.0152 389.4102 Origanum oil 5: Menthol 5 15.6681 333.2025 93.7691 355.3438 Origanum oil 4: Menthol 6 32.1270 162.5004 92.5229 175.6327 Origanum oil 3: Menthol 7 30.7097 170.0000 91.2767 186.2470 Origanum oil 2: Menthol 8 36.9644 141.2345 90.0305 156.8741 Origanum oil 1: Menthol 9 33.1470 157.4999 88.7843 177.3962

[0088] As can be seen from Table 18, the combination of oregano oil and menthone exhibits excellent antibacterial activity against Sclerotium rolfsii. The co-toxicity coefficients of all tested combined mixtures against Sclerotium rolfsii are greater than 120, showing a synergistic effect.

[0089] Table 19 Indoor bioactivity determination of the combination of oregano oil and menthone against Pseudograminearum

[0090] Name and ratio of the medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 24.2976 100.0000 -- -- Menthol 80.5613 30.1604 -- -- Origanum oil 9: Menthol 1 16.4613 147.6044 93.0160 158.6870 Origanum oil 8: Menthol 2 23.1954 104.7518 86.0321 121.7590 Origanum oil 7: Menthol 3 25.0967 96.8159 79.0481 122.4772 Origanum oil 6: Menthol 4 21.5619 112.6877 72.0642 156.3713 Origanum oil 5: Menthol 5 15.4636 157.1277 65.0802 241.4371 Origanum oil 4: Menthol 6 15.9469 152.3657 58.0962 262.2643 Origanum oil 3: Menthol 7 30.0176 80.9445 51.1123 158.3661 Origanum oil 2: Menthol 8 17.2011 141.2561 44.1283 320.1031 Origanum oil 1: Menthol 9 30.0176 80.9445 37.1443 217.9188

[0091] As can be seen from Table 19, the combination of oregano oil and menthone exhibits excellent antibacterial activity against Pseudograminearum. The co-toxicity coefficients of all tested combined mixtures against Sclerotium rolfsii are greater than 120, showing a synergistic effect.

[0092] Table 20 Indoor bioactivity determination of the combination of oregano oil and menthone against Rhizoctonia solani

[0093] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 17.4320 100.0000 -- -- Menthol 30.6867 56.8082 -- -- Origanum oil 9: Menthol 1 11.2171 155.4056 95.6808 162.4208 Origanum oil 8: Menthol 2 10.0779 172.9725 91.3616 189.3273 Origanum oil 7: Menthol 3 15.7313 110.8109 87.0425 127.3067 Origanum oil 6: Menthol 4 10.7497 162.1627 82.7233 196.0302 Origanum oil 5: Menthol 5 8.5428 204.0549 78.4041 260.2604 Origanum oil 4: Menthol 6 14.9996 116.2164 74.0849 156.8692 Origanum oil 3: Menthol 7 28.0428 62.1621 69.7658 89.1012 Origanum oil 2: Menthol 8 30.7135 56.7568 65.4466 86.7223 Origanum oil 1: Menthol 9 31.1482 55.9647 61.1274 91.5542

[0094] As can be seen from Table 20, the combination of oregano oil and menthone exhibits excellent antibacterial activity against Rhizoctonia solani. When the mixing ratio of oregano oil to menthone is 9-4:1-6, the co-toxicity coefficient of the combined mixture against Rhizoctonia solani is greater than 120, showing a synergistic effect.

[0095] Table 21 Indoor bioactivity determination of the combination of oregano oil and menthone against Fusarium graminearum

[0096] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 27.8794 100.0000 -- -- Menthol 58.2502 47.8615 -- -- Origanum oil 9: Menthol 1 16.9600 164.3833 94.7861 173.4254 Origanum oil 8: Menthol 2 21.8838 127.3974 89.5723 142.2286 Origanum oil 7: Menthol 3 26.7789 104.1096 84.3584 123.4134 Origanum oil 6: Menthol 4 20.7673 134.2466 79.1446 169.6220 Origanum oil 5: Menthol 5 18.1714 153.4246 73.9307 207.5248 Origanum oil 4: Menthol 6 18.8444 147.9453 68.7169 215.2969 Origanum oil 3: Menthol 7 25.4400 109.5888 63.5030 172.5726 Origanum oil 2: Menthol 8 18.3351 152.0548 58.2892 260.8629 Origanum oil 1: Menthol 9 27.5027 101.3697 53.0753 190.9921

[0097] As can be seen from Table 21, the compound of oregano oil and menthone shows excellent antibacterial activity against Fusarium graminearum. The co-toxicity coefficients of all tested compound combinations against Fusarium graminearum are greater than 120, showing a synergistic effect.

[0098] Table 22 Indoor bioactivity determination of the compound of oregano oil and menthone against Fusarium incarnatum

[0099] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 14.7602 100.0000 -- -- Menthol 110.2090 13.3929 -- -- Origanum oil 9: Menthol 1 10.2933 143.3962 91.3393 156.9929 Origanum oil 8: Menthol 2 21.7303 67.9245 82.6786 82.1549 Origanum oil 7: Menthol 3 27.9398 52.8286 74.0179 71.3727 Origanum oil 6: Menthol 4 41.1732 35.8490 65.3572 54.8510 Origanum oil 5: Menthol 5 8.5966 171.6981 56.6965 302.8375 Origanum oil 4: Menthol 6 9.3130 158.4903 48.0358 329.9423 Origanum oil 3: Menthol 7 78.2291 18.8679 39.3750 47.9185 Origanum oil 2: Menthol 8 15.9651 92.4529 30.7143 301.0090 Origanum oil 1: Menthol 9 260.7635 5.6604 22.0536 25.6664

[0100] As can be seen from Table 22, the compound of oregano oil and menthone shows excellent antibacterial activity against Fusarium incarnatum. When the compound ratio of oregano oil and menthone is 9:1, 5:5, 4:6 and 2:8, the co-toxicity coefficients of the compound combinations against Fusarium incarnatum are all greater than 120, showing a synergistic effect.

[0101] Table 23 Indoor bioactivity determination of the compound of oregano oil and menthone against Fusarium equiseti

[0102] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 27.1109 100.0000 -- -- Menthol 145.5956 18.6207 -- -- Origanum oil 9: Menthol 1 13.9541 194.2863 91.8621 211.4978 Origanum oil 8: Menthol 2 26.3578 102.8572 83.7241 122.8525 Origanum oil 7: Menthol 3 39.5367 68.5715 75.5862 90.7196 Origanum oil 6: Menthol 4 28.7540 94.2857 67.4483 139.7896 Origanum oil 5: Menthol 5 12.9954 208.6192 59.3103 351.7417 Origanum oil 4: Menthol 6 12.8227 211.4290 51.1724 413.1698 Origanum oil 3: Menthol 7 35.1438 77.1428 43.0345 179.2580 Origanum oil 2: Menthol 8 13.3645 202.8576 34.8965 581.3113 Origanum oil 1: Menthol 9 28.7540 94.2857 26.7586 352.3563

[0103] As can be seen from Table 23, the compound of oregano oil and menthone shows excellent antibacterial activity against Fusarium equiseti. When the compound ratio of oregano oil and menthone is 7:3, the compound combination shows an additive effect against Fusarium equiseti, and the co-toxicity coefficients of the other compound combinations against Fusarium equiseti are all greater than 120, showing a synergistic effect.

[0104] Table 24 Indoor bioactivity determination of the compound of oregano oil and menthone against Fusarium proliferatum

[0105] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 30.5120 100.0000 -- -- Menthol 114.0774 26.7468 -- -- Origanum oil 9: Menthol 1 14.8073 206.0605 92.6747 222.3483 Origanum oil 8: Menthol 2 29.6146 103.0303 85.3494 120.7159 Origanum oil 7: Menthol 3 38.7268 78.7878 78.0240 100.9789 Origanum oil 6: Menthol 4 27.9693 109.0910 70.6987 154.3042 Origanum oil 5: Menthol 5 15.9825 190.9088 63.3734 301.2445 Origanum oil 4: Menthol 6 16.2403 187.8783 56.0481 335.2093 Origanum oil 3: Menthol 7 29.6146 103.0303 48.7227 211.4624 Origanum oil 2: Menthol 8 12.5862 242.4242 41.3974 585.6026 Origanum oil 1: Menthol 9 28.7685 106.0604 34.0721 311.2826

[0106] As can be seen from Table 24, the compound of oregano oil and menthone shows excellent antibacterial activity against Fusarium proliferatum. When the compound ratio of oregano oil and menthone is 7:3, the compound combination shows an additive effect against Fusarium proliferatum; the other compound combinations show a synergistic effect against Fusarium proliferatum.

[0107] Table 25 Indoor bioactivity determination of the compound of oregano oil and menthone against Fusarium oxysporum

[0108] Name and ratio of medicament <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Origanum oil 31.4610 100.0000 -- -- Menthol 144.7977 21.7276 -- -- Origanum oil 9: Menthol 1 14.5566 216.1288 92.1728 234.4823 Origanum oil 8: Menthol 2 27.8655 112.9031 84.3455 133.8578 Origanum oil 7: Menthol 3 32.5097 96.7742 76.5183 126.4720 Origanum oil 6: Menthol 4 22.1657 141.9355 68.6910 206.6289 Origanum oil 5: Menthol 5 15.4808 203.2259 60.8638 333.9029 Origanum oil 4: Menthol 6 16.8154 187.0964 53.0365 352.7688 Origanum oil 3: Menthol 7 30.4778 103.2260 45.2093 228.3291 Origanum oil 2: Menthol 8 13.9327 225.8069 37.3820 604.0518 Origanum oil 1: Menthol 9 32.5097 96.7742 29.5548 327.4399

[0109] As can be seen from Table 25, the compound of oregano oil and menthone shows excellent antibacterial activity against Fusarium oxysporum. The co-toxicity coefficients of all tested compound combinations against Fusarium oxysporum are greater than 120, showing a synergistic effect. Among them, when the compound ratio of oregano oil and menthone is 2:8, the co-toxicity coefficient reaches 604.0518, showing a significant synergistic effect.

[0110] In summary, the present invention combines honokiol, carvacrol or oregano oil with menthone in a certain mass, showing a synergistic effect on the pathogenic bacteria of various plant fungal diseases, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and is of great significance for the high-quality and high-yield of crops such as wheat, peanut and maize.

[0111] The foregoing description of specific exemplary embodiments of the invention has been presented for purposes of illustration and example. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that, according to the above teaching, many changes and variations are possible. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical application, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and changes. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. Application of a bactericidal composition containing menthone in preventing and controlling crop diseases, wherein the crop diseases are caused by Sclerotium rolfsii, Rhizoctonia solani, Fusarium graminearum, and Fusarium oxysporum. It is characterized in that the active ingredient of the bactericidal composition containing menthone is compounded by magnolol, carvacrol or oregano oil and menthone; when preventing and controlling crop diseases caused by Sclerotium rolfsii, the mass ratio of magnolol to menthone is 9 - 1:1 - 9; the mass ratio of carvacrol to menthone is 7:3, 6:4, and 5:5; the mass ratio of oregano oil to menthone is 9 - 1:1 - 9; when preventing and controlling crop diseases caused by Rhizoctonia solani, the mass ratio of magnolol to menthone is 9 - 5:1 - 5; the mass ratio of carvacrol to menthone is 7:3; the mass ratio of oregano oil to menthone is 9 - 4:1 - 6; when preventing and controlling crop diseases caused by Fusarium graminearum, the mass ratio of magnolol to menthone is 9 - 1:1 - 9; the mass ratio of carvacrol to menthone is 7 - 1:3 - 9; the mass ratio of oregano oil to menthone is 9 - 1:1 - 9; when preventing and controlling crop diseases caused by Fusarium oxysporum, the mass ratio of magnolol to menthone is 9 - 1:1 - 9; the mass ratio of carvacrol to menthone is 3:7 and 1:9; the mass ratio of oregano oil to menthone is 9 - 1:1 - 9.