Microfluidic-based fluid control methods, devices, and microfluidic systems

Abstract

The application provides a microfluidic-based fluid control method, device and system, relates to the technical field of microfluidics, and the method comprises the following steps: monitoring current interface position information of a laminar flow interface in a confluence flow channel, a first liquid flowing out from a first branch flow channel and a second liquid flowing out from a second branch flow channel confluencing to form a laminar flow in the confluence flow channel, and the laminar flow interface being an interface between the first liquid and the second liquid in the confluence flow channel; if the current interface position information does not match target interface position information corresponding to a current working mode, adjusting at least one of a flow rate of the first liquid and a flow rate of the second liquid until updated current interface position information matches the target interface position information, so as to form a target fluid in the confluence flow channel, and the target interface position information is used for indicating an expected position of the laminar flow interface in the confluence flow channel under the current working mode.

Description

Technical Field

[0001] This application relates to the field of microfluidics, and in particular to a fluid control method, apparatus and microfluidics system based on microfluidics. Background Technology

[0002] Microfluidics refers to the technology of processing or manipulating tiny fluids using micrometer-scale microchannels. It involves the intersection of knowledge from chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering, and has enormous development potential and broad application prospects in fields such as biomedical research. In the application of microfluidic devices, to ensure detection effectiveness, the flow rate of the liquid within the channel needs to be precisely controlled to meet detection requirements. However, due to the minute characteristics of microfluidic systems, the detection and control of the flow rate of the analyte is difficult, resulting in low control precision and hindering detection accuracy. Therefore, an improved solution is needed to address at least one of the aforementioned problems. Summary of the Invention

[0003] This application provides a fluid control method, device, and microfluidic system based on microfluidics, which can significantly reduce the accuracy requirements, difficulty, and complexity of fluid regulation in microfluidics, and improve detection accuracy and efficiency.

[0004] On one hand, this application provides a microfluidic-based fluid control method applied to a microfluidic device, the microfluidic device being provided with a first branch channel, a second branch channel, and a converging channel, the converging channel being connected to the first branch channel and the second branch channel respectively, the method comprising:

[0005] The current interface position information of the laminar flow interface in the confluence channel is monitored. The first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar flow interface is the interface between the first liquid and the second liquid in the confluence channel.

[0006] If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, so as to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar flow interface in the confluence channel under the current working mode.

[0007] In a possible implementation, the target interface location information is determined in the following way: the target interface location information corresponding to the current working mode is determined based on a first preset correspondence, wherein the first preset correspondence is used to characterize the correspondence between multiple working modes of the microfluidic device and the preset location information.

[0008] In a possible implementation, adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes:

[0009] Based on the target interface position information and the current interface position information, determine the offset direction of the laminar flow interface relative to the desired position;

[0010] If the offset direction is the laminar flow interface shifting towards the first branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid, until the laminar flow interface moves towards the second branch channel side until the updated current interface position information matches the target interface position information.

[0011] In a possible implementation, the method further includes: if the offset direction is the laminar interface shifting towards the second branch channel side, adjusting at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid, until the laminar interface moves towards the first branch channel side until the updated current interface position information matches the target interface position information.

[0012] In a possible implementation, before adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, the method further includes:

[0013] Obtain the current flow rate of the second liquid; determine the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid.

[0014] In a possible implementation, adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes:

[0015] Based on the second preset correspondence, the current flow rate ratio corresponding to the current interface position information and the target flow rate ratio corresponding to the target interface position information are determined. The second preset correspondence is used to characterize the correspondence between the preset flow rate ratio and the multiple interface position information of the laminar flow interface in the converging flow channel.

[0016] Based on the current flow rate of the second liquid and the current flow rate of the first liquid, the flow rate of at least one of the first liquid and the second liquid is adjusted until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information matches the target interface position information.

[0017] In a possible implementation, the microfluidic device further includes a photosensitive sensor, the signal acquisition area of ​​which covers at least a portion of the confluence channel, for generating photosensitive information of the laminar flow in the confluence channel; the current interface position information is determined in the following manner:

[0018] Acquire the photosensitized information collected by the optical sensor;

[0019] Based on the photosensitive information, the interface position is analyzed to obtain the current interface position information.

[0020] In a possible implementation, the light sensor is a camera, and the photosensitive information is a flow channel image acquired for the converging flow channel; the step of performing interface position analysis based on the photosensitive information to obtain the current interface position information includes:

[0021] Edge detection is performed on the flow channel image of the confluence flow channel and the laminar flow interface in the confluence flow channel to obtain the first edge corresponding to the first sidewall, the second edge corresponding to the second sidewall and the third edge corresponding to the laminar flow interface. The first sidewall and the second sidewall are two walls that are arranged opposite to each other on the confluence flow channel.

[0022] The current interface position information is determined based on the first distance between the first edge and the third edge, and the second distance between the second edge and the third edge.

[0023] In a possible implementation, the light sensor is a camera, and the photosensitive information is a flow channel image acquired for the converging flow channel; the step of performing interface position analysis based on the photosensitive information to obtain the current interface position information includes:

[0024] Based on the flow channel image, obtain the coordinate information of multiple feature points on the boundary line corresponding to the laminar flow interface;

[0025] Based on the coordinate information of the multiple feature points, the slope of the boundary line is determined;

[0026] The current interface position information is determined based on the slope of the dividing line.

[0027] In a possible implementation, the flow rate ratio is controlled in the following manner:

[0028] The flow rate of the first liquid in the first branch channel is controlled to be stable, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio.

[0029] If the flow rate of the second liquid in the second branch channel reaches the upper limit or lower limit of the second liquid flow rate, the flow rate of the second liquid in the second branch channel is stabilized, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio.

[0030] In a possible implementation, the flow rate ratio is controlled in the following manner:

[0031] The flow rate of the second liquid in the second branch channel is controlled to be stable, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio.

[0032] If the flow rate of the first liquid in the first branch channel reaches the upper limit or lower limit of the first liquid flow rate, the flow rate of the first liquid in the first branch channel is stabilized, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio.

[0033] In a possible implementation, the flow rate ratio is controlled in the following manner: if it is necessary to reduce the flow rate ratio between the first liquid and the second liquid, the flow rate of the second liquid in the second branch channel is controlled to be stable, and the flow rate of the first liquid in the first branch channel is reduced.

[0034] In a possible implementation, the flow rate ratio is controlled in the following manner:

[0035] If it is necessary to increase the flow rate ratio between the first liquid and the second liquid, control the flow rate of the first liquid in the first branch channel to stabilize, and decrease the flow rate of the second liquid in the second branch channel.

[0036] In a possible implementation, at least one of the first branch flow channel and the second branch flow channel is connected to a flow regulating device, and the flow rate of the first liquid or the flow rate of the second liquid is regulated in the following manner:

[0037] Adjust the operating parameters of the flow regulating device to regulate the flow rate of the first liquid in the first branch channel connected to the flow regulating device or the flow rate of the second liquid in the second branch channel connected to the flow regulating device.

[0038] In a possible implementation, adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes:

[0039] Under the first flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information;

[0040] The first flow velocity constraint condition is used to control the flow velocity of the first liquid within a first flow velocity range, the first flow velocity range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel; and / or, the first flow velocity constraint condition is used to control the flow velocity of the second liquid within a second flow velocity range, the second flow velocity range being the fluid velocity range required to maintain the laminar flow state of the second liquid in the second branch channel and the confluence channel.

[0041] In a possible implementation, the first liquid is blood, and adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes:

[0042] Under the second flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information;

[0043] The second flow velocity constraint is used to control the flow velocity of the first liquid within a third flow velocity range, which is the fluid velocity range required to maintain the first liquid in a laminar flow state in the first branch channel and the confluence channel, and to prevent the first liquid from forming a coagulation state.

[0044] In a possible implementation, the microfluidic device further includes a first detection device and a mixing channel communicating with the confluence channel. In the current operating mode, which is a mixed liquid detection mode, the second liquid is a reaction solution capable of specifically reacting with the first liquid. The mixing channel is used to mix the target fluid so that the reaction solution mixes with the first liquid and undergoes a specific reaction to form a target mixed liquid.

[0045] After adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel, the method further includes:

[0046] The target mixture is tested using the first detection device to obtain first detection parameter data, which is used to indicate the content information of the target substance in the target mixture.

[0047] The detection result is generated based on the first detection parameter data.

[0048] In a possible implementation, when the current working mode is the mixed liquid detection mode, the target interface position information is determined based on a preset mixing ratio between the first liquid and the second liquid.

[0049] In a possible implementation, the first liquid is blood;

[0050] When the target substance includes an anticoagulant, the first detection parameter data includes detection data indicating the anticoagulant level of the blood.

[0051] In a possible implementation, the method further includes:

[0052] Obtain the interface position information corresponding to the target mixture;

[0053] Based on the interface position information corresponding to the target mixture, the actual mixing ratio between the first liquid and the second liquid is determined.

[0054] The first liquid dilution factor corresponding to the target mixture is determined based on the actual mixing ratio.

[0055] The step of generating the detection result based on the first detection parameter data includes:

[0056] The detection result is generated based on the first detection parameter data and the first liquid dilution factor.

[0057] In a possible implementation, the microfluidic device further includes a second detection device disposed on the confluence channel, wherein the second liquid is a reference solution;

[0058] In the current working mode, which is the cleaning mode for the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface is deviated from the first branch channel side and the reference solution covers the detection area of ​​the second detection device.

[0059] In a possible implementation, the microfluidic device further includes a second detection device disposed on the confluence channel;

[0060] In the current working mode, which is the measurement mode based on the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface deviates towards the second branch channel and the first liquid covers the detection area of ​​the second detection device.

[0061] In a possible implementation, in the cleaning mode, after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form the target fluid in the confluence channel, the method further includes:

[0062] Acquire preset parameter data of the reference solution and second detection parameter data of the second detection device, wherein the second detection parameter data is used to indicate the content information of the target marker in the reference solution flowing through the second detection device;

[0063] If the preset parameter data is consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

[0064] In a possible implementation, the method further includes:

[0065] If the preset parameter data is inconsistent with the second detection parameter data, and the second detection parameter data remains stable, adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid.

[0066] If the preset parameter data is found to be consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

[0067] In a possible implementation, the method further includes:

[0068] When the preset parameter data and the updated second detection parameter data are consistent, the updated interface position information is obtained;

[0069] Based on the updated interface position information, calibrate the target interface position information corresponding to the current working mode.

[0070] In a possible implementation, in the measurement mode, after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form the target fluid in the confluence channel, the method further includes:

[0071] Acquire third detection parameter data of the second detection device, wherein the third detection parameter data is used to indicate the content information of the target marker in the first liquid flowing through the second detection device;

[0072] If the third detection parameter data remains stable within a preset time period, a detection result is generated based on the third detection parameter data.

[0073] In a possible implementation, the method further includes:

[0074] If the third detection parameter data is unstable within a preset time period, at least one of the flow rates of the first liquid and the second liquid is adjusted to increase the flow rate ratio between the first liquid and the second liquid until the updated third detection parameter data remains stable within the preset time period.

[0075] The detection results are generated based on the updated third detection parameter data.

[0076] On the other hand, a microfluidic-based fluid control device is provided, applied to a microfluidic device. The microfluidic device is provided with a first branch channel, a second branch channel, and a converging channel. The converging channel is respectively connected to the first branch channel and the second branch channel. The device includes:

[0077] Interface monitoring module: used to monitor the current interface position information of the laminar flow interface in the confluence channel; the first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow, and the laminar flow interface is the interface between the first liquid and the second liquid in the confluence channel;

[0078] Speed ​​control module: If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, so as to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar flow interface in the confluence channel under the current working mode.

[0079] On the other hand, a microfluidic system is provided, including a microfluidic device and a fluid control device. The microfluidic device is provided with a first branch channel, a second branch channel and a converging channel, and the converging channel is respectively connected to the first branch channel and the second branch channel.

[0080] The fluid control device is used to monitor the current interface position information of the laminar interface in the confluence channel. The first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar interface is the interface between the first liquid and the second liquid in the confluence channel. If the current interface position information does not match the target interface position information corresponding to the current operating mode, at least one of the flow rates of the first liquid and the second liquid is adjusted until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar interface in the confluence channel under the current operating mode.

[0081] On the other hand, an electronic device is provided, the device including a processor and a memory, the memory storing at least one instruction or at least one program, the at least one instruction or the at least one program being loaded and executed by the processor to implement the microfluidics-based fluid control method as described above.

[0082] On the other hand, a computer-readable storage medium is provided, wherein at least one instruction or at least one program is stored therein, the at least one instruction or the at least one program being loaded and executed by a processor to implement the microfluidic-based fluid control method as described above.

[0083] On the other hand, a microfluidic device is provided, which includes a processor and a memory, wherein the memory stores at least one instruction or at least one program, and the at least one instruction or the at least one program is loaded and executed by the processor to implement the microfluidic-based fluid control method as described above.

[0084] On the other hand, a computer program product or computer program is provided, which includes computer instructions that, when executed by a processor, implement the microfluidic-based fluid control method as described above.

[0085] The microfluidic-based fluid control method, apparatus, device, storage medium, microfluidic device, computer program, and computer program product provided in this application have the following technical advantages:

[0086] The technical solution of this application can be applied to microfluidic devices. In the microfluidic device, a first liquid flowing out from a first branch channel and a second liquid flowing out from a second branch channel merge in a confluence channel to form laminar flow and create a laminar interface between the first and second liquids. By monitoring the current interface position information of the laminar interface in the confluence channel and comparing it with the target interface position information corresponding to the current working mode, if they do not match, at least one of the flow rates of the first and second liquids is adjusted to move the laminar interface toward the desired position corresponding to the target interface position information. This process continues until the updated current interface position information matches the target interface position information, thus obtaining the target laminar flow required for the current working mode. During the control process, the laminar interface position can be adjusted by adjusting the flow rates of the first and / or second liquids, thereby achieving the preset position. There is no need to precisely determine and adjust the specific flow rate values ​​of the liquids, reducing the difficulty of control. Furthermore, the technical solution of this application does not require calibration of the relationship between different channels and flow rate data, is not affected by changes in the shape or size of the channel pipes, and has a wide range of applications. Attached Figure Description

[0087] To more clearly illustrate the technical solutions and advantages in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0088] Figure 1 This is a schematic diagram of a microfluidic-based fluid processing system provided in an embodiment of this application;

[0089] Figure 2 This is a schematic diagram of a Y-shaped microfluidic channel provided in an embodiment of this application;

[0090] Figure 3 This is a schematic diagram of an H-type microfluidic channel provided in an embodiment of this application;

[0091] Figure 4 This is a schematic flowchart of a microfluidic-based fluid control method provided in an embodiment of this application;

[0092] Figure 5 This is a schematic diagram of another microfluidic channel structure provided in an embodiment of this application;

[0093] Figure 6 This is a schematic diagram of laminar flow changes in the confluence channel under the cleaning and measurement modes provided in the embodiments of this application;

[0094] Figure 7 This is a schematic flowchart of another microfluidic-based fluid control method provided in an embodiment of this application;

[0095] Figure 8 This is a schematic flowchart of another microfluidic-based fluid control method provided in an embodiment of this application;

[0096] Figure 9 This is a schematic flowchart of another microfluidic-based fluid control method provided in an embodiment of this application;

[0097] Figure 10 This is a schematic flowchart of another microfluidic-based fluid control method provided in an embodiment of this application;

[0098] Figure 11 This is a schematic diagram of the framework of a microfluidic-based fluid control device provided in an embodiment of this application;

[0099] Figure 12 This is a hardware structure block diagram of an electronic device that performs a microfluidic-based fluid control method or neural network training method, as provided in an embodiment of this application. Detailed Implementation

[0100] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0101] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of this application described herein can be implemented in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or server that comprises a series of steps or sub-modules is not necessarily limited to those steps or sub-modules explicitly listed, but may include other steps or sub-modules not explicitly listed or inherent to such processes, methods, products, or devices.

[0102] Before providing a further detailed description of the embodiments of this application, the nouns and terms involved in the embodiments of this application will be explained, and the nouns and terms involved in the embodiments of this application shall be interpreted as follows.

[0103] Blood clotting, or coagulation, refers to the process by which blood changes from a liquid state to a non-flowing gel state, and is an important step in physiological hemostasis. Essentially, blood clotting is the process by which soluble fibrinogen in plasma is converted into insoluble fibrin.

[0104] Coagulation factors are substances involved in the blood clotting process. Except for two newly discovered factors, the remaining coagulation factors are named using Roman numerals by the International Committee on the Nomenclature of Blood Clotting Factors, in the order of their discovery. Activated coagulation factors are indicated by the letter "a" to the lower right of their names. Their physiological function is to be activated during bleeding, adhering to platelets and sealing leaks in blood vessels. For standardized naming, the World Health Organization uses Roman numerals to number them according to their order of discovery: coagulation factors I, II, III, IV, V, VII, VIII, IX, X, XI, XII, XIII, XIII, etc.

[0105] Heparin is an effective anticoagulant widely used for the prevention and treatment of acute thrombosis.

[0106] Anti-Xa factor, the level of anti-Xa factor (anti-Xa) is usually used to monitor intravenous heparin (unfractionated heparin UFH or low molecular weight heparin LMWH) unfractionated heparin (IV heparin).

[0107] Please see Figure 1 The illustration shows a microfluidic fluid handling system according to an exemplary embodiment, including a control host 01, a first flow regulating device 02, a second flow regulating device 03, a microfluidic channel 04, and a detection device 05. The control host 01 is communicatively connected to the first flow regulating device 02, the second flow regulating device 03, and the detection device 05, and can control the liquid delivery speed corresponding to each of the first and second flow regulating devices 02 and 03. The microfluidic channel 04 includes an inlet channel, a confluence channel, and an outlet channel connected in sequence. The inlet channel may include a first branch channel and a second branch channel. The first flow regulating device 02 and the second flow regulating device 03 may include, but are not limited to, peristaltic pumps and syringe pumps. The first flow regulating device 02 is used to input a first liquid into the first branch channel, and the second flow regulating device 03 is used to input a second liquid into the second branch channel. The first liquid enters the confluence channel via the first branch channel, and the second liquid enters the confluence channel via the second branch channel, forming a confluence liquid in the confluence channel. The confluence channel can be equipped with a corresponding detection device 05. The detection device 05 is used to detect parameters of the liquid in the confluence channel. The detection device 05 can be a contact or non-contact device relative to the confluence channel. When the detection device 05 is a non-contact device, it can include, but is not limited to, devices such as photoelectric sensors; when the detection device 05 is a contact device, it can include, but is not limited to, devices such as inductive sensors, resistive sensors, and piezoelectric sensors.

[0108] In an exemplary embodiment, when neither the first liquid nor the second liquid needs to be recycled, the outlet flow channel can be a single flow channel. In this case, such as... Figure 2 As shown, the microfluidic channel 04 is Y-shaped. The confluence of the two liquids enters the outlet channel through the confluence channel and is discharged as waste liquid. In an exemplary embodiment, when the second liquid and the first liquid need to be recycled, the outlet channel may include a third branch channel and a fourth branch channel, wherein the third branch channel and the first branch channel are located on the same side of the confluence channel, and the fourth branch channel and the second branch channel are located on the other side of the confluence channel; at this time, as Figure 3As shown, the microfluidic channel 04 is H-shaped. The reference solution flows out from the third branch channel via the confluence channel, and the first liquid flows out from the fourth branch channel via the confluence channel. In applications where in vitro blood sampling tests are performed without the need to recover the blood after testing, this method can be used... Figure 2 The microfluidic channel shown can be used when blood is processed outside the body and then returned to the body. Figure 3 The microfluidic channel is shown. In a microfluidic device with a Y-shaped channel, the output ends of the first branch channel and the second branch channel are respectively connected to the input end of the converging channel.

[0109] In another exemplary embodiment, reference is made to Figure 5 The microfluidic channel includes a first branch channel 041, a second branch channel 042, a confluence channel 043, a mixing channel 044, and an auxiliary channel for splitting the mixture. The first branch channel 041 is used to input a first liquid, the second branch channel 042 is used to input a second liquid, and the input end of the confluence channel 043 is connected to the first branch channel 041 and the second branch channel 042. A corresponding detection device 05 is installed outside the auxiliary channel. The mixing channel 044 has a bent pipe structure, and the confluence channel 043 has a straight pipe structure. The auxiliary channel may include an inlet... The liquid inlet 045 and the liquid inlet branch channels 046 and 047 connected to the liquid inlet allow the slitting solution to enter the two liquid inlet branch channels through the liquid inlet. The outlets of the two liquid inlet branch channels are connected to the output end of the confluence channel 043. The second liquid and the first liquid form a laminar flow in the confluence channel 043 and flow into the mixing channel 044, where they mix to form a mixed liquid. The mixed liquid then enters the detection section 048 of the auxiliary channel, where the slitting solution in the auxiliary channel slits the mixed liquid into droplets. The detection device 05 is a photoelectric sensor, which detects the relevant parameters of the droplets.

[0110] When the mixing channel 043 is present, the output end of the confluence channel is connected to the input end of the mixing channel 043, and the laminar fluid in the confluence channel is mixed evenly through the mixing channel 043 to form a mixed liquid.

[0111] Understandable, Figures 1-3 The examples shown are merely a few examples of systems and structures related to microfluidic devices. Many more types and structures of microfluidic devices and fluid handling systems may be included, and this application does not impose any limitations on them.

[0112] The technical solution of this application is described below in conjunction with the aforementioned system and microfluidic channel. The embodiments of this application can be applied to various fluid processing scenarios based on microfluidic technology, including but not limited to fluid parameter detection and adding target substances to fluids. Please refer to... Figure 4 , Figure 4This is a flowchart illustrating a microfluidic-based fluid control method provided in this application. This specification provides the method operation steps as shown in the embodiments or flowcharts, but based on conventional or non-inventive methods, more or fewer operation steps may be included. The order of steps listed in the embodiments is merely one possible execution order among many and does not represent the only possible execution order. In actual system or server product execution, the method can be executed sequentially according to the embodiments or drawings, or in parallel (e.g., in a parallel processor or multi-threaded processing environment). Specifically, as... Figure 4 As shown, the microfluidic-based fluid control method of this application is applied to a microfluidic device. The microfluidic device is provided with a first branch channel, a second branch channel, and a converging channel. The converging channel is connected to the first branch channel and the second branch channel, respectively. The method may include the following steps S201-S203:

[0113] S201: Monitor the current interface position information of the laminar flow interface in the confluence channel.

[0114] Specifically, the first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar interface is the boundary between the first liquid and the second liquid in the confluence channel. The current interface position information is used to indicate the current position of the laminar interface in the confluence channel. Specifically, the surface where the second liquid contacts the first liquid is the interface. In this embodiment, the laminar flow in the confluence channel can be detected at the interface to obtain the current interface position information. The current interface position information can be the interface position information of the current detection node. The interface position information of different detection nodes can be the same or different. The interface position information can characterize the position information of the laminar interface in the confluence channel. The boundary line formed by the laminar interface can be parallel to or intersect with the channel wall of the confluence channel. Specifically, at the initial moment when the second liquid and the first liquid have different flow velocities and intersect (which could be at the input starting point of the confluence channel), the boundary line formed by the laminar interface between the second liquid and the first liquid intersects the channel wall of the confluence channel. Furthermore, when the second liquid and the first liquid enter the confluence channel and tend to stabilize, the boundary line formed by the laminar interface between the second liquid and the first liquid is parallel to the channel wall of the confluence channel. When the second liquid and the first liquid have the same flow velocity, the boundary line formed by the laminar interface between the second liquid and the first liquid is parallel to the confluence channel. At the initial moment when the second liquid and the first liquid intersect (which could be at the input starting point of the confluence channel), the boundary line formed by the laminar interface between the second liquid and the first liquid is parallel to the channel wall of the confluence channel. When the second liquid and the first liquid enter the confluence channel and tend to stabilize, the boundary line formed by the laminar interface between the second liquid and the first liquid is also parallel to the channel wall of the confluence channel.

[0115] Understandably, one of the first liquid and the second liquid can be the liquid to be tested, and the other can be a preset liquid. For ease of description, the embodiments of this application use the first liquid as the liquid to be tested and the second liquid as the preset liquid to illustrate the technical solution.

[0116] Specifically, the first liquid can be determined based on the actual application scenario. For example, in a blood testing scenario, the first liquid can be blood. The second liquid can be a reference solution or a reaction solution. The reference solution and the first liquid are chemically inert to each other, while the reaction solution and the first liquid can undergo a specific reaction.

[0117] In this embodiment, the microfluidic device further includes a photosensitive sensor, the signal acquisition area of ​​which covers at least a portion of the confluence channel, for generating photosensitive information of the laminar flow in the confluence channel; the current interface position information is determined in the following manner:

[0118] S2011: Acquire the photosensitized information collected by the optical sensor;

[0119] S2012: Based on the photosensitive information, perform interface position analysis to obtain the current interface position information.

[0120] Specifically, the light signals or information generated by the reflection or refraction of light in the confluence channel, and the reflection and refraction of light beams at the laminar flow interface are different from those in other parts of the confluence channel, the light sensor can receive the light signals or light information to generate photosensitive information of the confluence channel and transmit it to the channel control device so that the channel control device can obtain the interface position information.

[0121] In some embodiments, the light sensor is a camera, and the photosensitive information is a flow channel image acquired for the confluence flow channel; the flow channel image includes at least the two end sidewalls of the confluence flow channel and the liquid inside the confluence flow channel, that is, S2012 may specifically include S20121-S20122:

[0122] S20121: Perform edge detection on the flow channel image of the confluence flow channel and the laminar flow interface in the confluence flow channel to obtain the first edge corresponding to the first sidewall, the second edge corresponding to the second sidewall and the third edge corresponding to the laminar flow interface. The first sidewall and the second sidewall are two walls set opposite to each other on the confluence flow channel.

[0123] S20122: Determine the current interface position information based on the first distance between the first edge and the third edge, and the second distance between the second edge and the third edge.

[0124] Specifically, the first sidewall can be the sidewall of the confluence channel on the side of the first branch channel, and the second sidewall can be the sidewall of the confluence channel on the side of the second branch channel. When the flow velocities of the second liquid and the first liquid are the same, the boundary line formed by the laminar interface between the second liquid and the first liquid is parallel to the confluence channel. At the initial moment when the second liquid and the first liquid intersect (which can be at the input starting point of the confluence channel), the boundary line formed by the laminar interface between the second liquid and the first liquid is parallel to the channel wall of the confluence channel. When the second liquid and the first liquid enter the confluence channel and tend to stabilize, the boundary line formed by the laminar interface between the second liquid and the first liquid is also parallel to the channel wall of the confluence channel.

[0125] When the laminar interface is parallel to the channel wall of the converging channel, edge detection technology based on the channel image can be used to detect the edges of the channel wall of the converging channel and the laminar interface in the converging channel, so as to obtain the first edge and the second edge corresponding to the channel wall of the converging channel, and the third edge corresponding to the laminar interface. The distance between the first edge and the third edge can refer to the first vertical distance between the first edge and the third edge, and the distance between the second edge and the third edge can refer to the second vertical distance between the second edge and the third edge.

[0126] The interface position information can be determined directly by the first vertical distance and the second vertical distance, or by the ratio of the first vertical distance to the second vertical distance. By performing edge detection on the channel walls and interfaces of the converging flow channels, and determining the vertical distances from the two side pipe walls to the interface based on the edge detection results, the vertical distances from the two side pipe walls to the interface visually represent the laminar flow interface position. This enables interface position control and intuitive comparison with the desired position, allowing for timely feedback on velocity adjustment and improving the real-time performance and accuracy of control.

[0127] In other embodiments, S2012 may specifically include S20123-S20125:

[0128] S20123: Obtain the coordinate information of multiple feature points on the boundary line corresponding to the laminar flow interface based on the flow channel image;

[0129] S20124: Determine the slope of the boundary line based on the coordinate information of multiple feature points;

[0130] S20125: Determine the current interface position information based on the slope of the dividing line.

[0131] Specifically, at the initial moment when the flow velocities of the second liquid and the first liquid are different and the second liquid and the first liquid intersect (which may be at the input starting point of the confluence channel), the boundary line formed by the laminar interface of the second liquid and the first liquid intersects with the edge line corresponding to the channel wall of the confluence channel.

[0132] A coordinate system can be established with the starting point of the converging flow channels as the origin, thus obtaining the coordinate information of multiple feature points on the boundary line. These multiple feature points can include two feature points; the slope of the boundary line can be determined based on the coordinate information of these two feature points. Alternatively, multiple feature points can include multiple feature point groups, each containing two feature points. The slope corresponding to each feature point group can be calculated based on these two feature points. Further data smoothing can be performed on multiple feature point groups to obtain the slope of the boundary line. The position of the boundary line in the coordinate system can be determined by combining the slope and the coordinate information of the feature points, thereby obtaining the current interface position information.

[0133] For detecting interface location information, an interface prediction model can also be used for prediction. For example, the flow channel image can be input into the interface prediction model for prediction to obtain the interface prediction result, and then the current interface location information can be determined.

[0134] S203: If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, so as to form the target fluid in the converging flow channel.

[0135] Specifically, the updated current interface position information refers to the interface position information obtained by updating the detection node during or after flow rate adjustment. During flow rate adjustment, the interface position information is continuously monitored to obtain multiple updated interface position information corresponding to the current interface information. The target interface position information is used to indicate the desired position of the laminar flow interface in the confluence channel under the current operating mode. Understandably, this can include multiple operating modes, including but not limited to mixed liquid detection mode, measurement mode, or cleaning mode, etc. The current operating mode is the currently triggered operating mode. The desired position may differ under different operating modes; for example, the desired position could be the radial center of the confluence channel, the position where the second liquid can cover the detection area of ​​the detection device on the confluence channel, or the position where the first liquid can cover the detection area of ​​the detection device on the confluence channel, etc. Accordingly, the method for determining the target interface location information can be as follows: the target interface location information corresponding to the current working mode is determined based on the first preset correspondence relationship. The first preset correspondence relationship is used to characterize the correspondence between multiple working modes of the microfluidic device and the preset location information. In this way, by pre-constructing the first preset correspondence relationship, the laminar flow interface control of different modes can be facilitated, thereby improving the working efficiency of the microfluidic device and reducing the complexity of operation.

[0136] Specifically, in the current operating mode, if the current interface position information does not match the target interface position information, liquid flow rate adjustment is performed to change the position of the laminar interface in the confluence channel. During the flow rate adjustment process, updated current interface position information is acquired and compared with the target interface information. If they match, it is determined that the laminar flow formed by the first and second liquids in the confluence channel meets the requirements of the current operating mode, forming the target fluid, and the flow rate adjustment of the first and / or second liquids is stopped. Conversely, if they do not match, the liquid flow rate adjustment continues until the updated current interface position information matches the target interface position information. After obtaining the target fluid, in the current operating mode, the current interface position information of the laminar interface can also be acquired periodically. If the laminar interface shifts, causing the current interface position information to not match the target interface position information, the flow rate adjustment operation of the first and / or second liquids is triggered until they match.

[0137] Specifically, the position of the laminar interface in the confluence channel is achieved by adjusting the flow rates of the first liquid and / or the second liquid. Understandably, flow rate adjustment leads to a change in the flow rate ratio. The smaller the flow rate ratio of the first liquid and the second liquid, the more the laminar interface moves towards the sidewall of the confluence channel on the first branch channel side; conversely, the smaller the flow rate ratio, the more the laminar interface moves towards the sidewall of the confluence channel on the second branch channel side. By adjusting the flow rates of the first liquid and / or the second liquid, the flow rate ratio between them is changed, thereby causing the laminar interface to move towards the desired channel side until the desired position corresponding to the target interface position information is reached. Specifically, in this application, the flow rate ratio = flow rate of the first liquid / flow rate of the second liquid.

[0138] Specifically, if the difference between the current interface location information and the target interface location information is less than or equal to a preset difference, the current interface location information and the target interface location information are determined to match; otherwise, they are not matched. For example, the difference in location information can be the distance between the current interface location and the target interface location and / or the slope difference between the current interface and the target interface.

[0139] In summary, the above-mentioned solution can adjust the laminar flow interface position by adjusting the flow rate of the first liquid and / or the second liquid during the control process, thereby achieving the preset position without the need to precisely determine and adjust the specific flow rate value of the liquid, thus reducing the difficulty of control. Furthermore, the technical solution of this application does not need to involve the relationship between different flow channels and flow rate data, is not affected by changes in the shape and size of the flow channel pipe, and has a wide range of applications.

[0140] Based on some or all of the above embodiments, in some embodiments, at least one of the first branch flow channel and the second branch flow channel is connected to a flow regulating device. Accordingly, the flow rate of the first liquid or the flow rate of the second liquid is regulated by adjusting the operating parameters of the flow regulating device to regulate the flow rate of the first liquid in the first branch flow channel connected to the flow regulating device or the flow rate of the second liquid in the second branch flow channel connected to the flow regulating device. Thus, the flow rate is regulated by directly controlling the operating parameters of the flow regulating device, eliminating the need for switching devices such as flow channel valves. The regulation method is simple and precise, and can be applied to high-frequency continuous monitoring or low-frequency intermittent monitoring.

[0141] Specifically, a flow regulating device can be controlled by a control host to deliver a corresponding liquid (first liquid or second liquid) into a microfluidic channel. There is a third correspondence between the operating parameters of the flow regulating device and the flow rate of the delivered liquid. When determining the desired target flow rate, the target operating parameters of the flow regulating device can be determined based on this third correspondence, and then the operating parameters of the flow regulating device can be adjusted to the target operating parameters to achieve the flow of the corresponding second liquid or first liquid into the microfluidic channel at the target flow rate. The operating parameters of the flow regulating device may specifically include parameters such as rotational speed and flow rate; the higher the rotational speed or the higher the flow rate, the higher the flow rate of the delivered liquid; conversely, the lower the rotational speed or the lower the flow rate, the lower the flow rate of the delivered liquid.

[0142] It should be noted that in this embodiment, when the first liquid or the second liquid flows into the microfluidic channel at a target flow rate based on the flow regulation device, the flow rate of the first liquid in the first branch channel and the confluence channel can be the target flow rate, or the flow rate of the second liquid in the second branch channel and the confluence channel can be the target flow rate. That is, the flow rate of the liquid will not change before entering the microfluidic channel and after entering the microfluidic channel.

[0143] When the fluid velocity is low, the fluid particles move in one dimension along the flow direction, and there is no macroscopic mixing with the surrounding fluid, i.e., stratified flow, and the fluid velocity is stable, this flow pattern is called laminar flow. In this embodiment, the second liquid and the first liquid flow into the confluence channel through the first branch channel and the second branch channel, respectively, so that laminar flow can be formed in the confluence channel; wherein the velocity of the first liquid can be stable but unknown.

[0144] Specifically, the flow regulating device can be, for example, a micro pump, such as a peristaltic pump or a syringe pump. The operating parameters can be the speed of the pump, etc. The higher the speed, the greater the flow rate, and vice versa.

[0145] Based on some or all of the above implementation methods, in some embodiments, if the target interface position information matches the current interface position information, it is determined that the laminar flow interface has no offset relative to the target position; otherwise, if they do not match, the flow rate adjustment method is determined according to the offset direction. Correspondingly, reference is made to... Figure 7 In S203, adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes the following S2031-S2033:

[0146] S2031: Based on the target interface position information and the current interface position information, determine the offset direction of the laminar flow interface relative to the desired position;

[0147] Specifically, if the current interface position of the laminar flow interface is detected to be between the desired position and the sidewall of the confluence channel on the first branch channel side, the offset direction is determined to be offset towards the first branch channel side; conversely, if it is between the desired position and the sidewall of the confluence channel on the second branch channel side, the offset direction is determined to be offset towards the second branch channel side.

[0148] S2032: If the offset direction is the laminar interface offset toward the first branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid, so that the laminar interface moves toward the second branch channel side until the updated current interface position information matches the target interface position information.

[0149] Specifically, the flow rate ratio is increased to increase the proportion of the first liquid in the confluence channel, which pushes the laminar interface toward the sidewall of the confluence channel on the side of the second branch channel. During the flow rate adjustment process, the position of the laminar interface is continuously monitored until the difference between the updated current interface position information and the target interface position information is less than or equal to the preset difference, at which point the control is stopped.

[0150] S2033: If the offset direction is the laminar interface shifting towards the second branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid, until the laminar interface moves towards the first branch channel side until the updated current interface position information matches the target interface position information.

[0151] Specifically, the flow rate ratio is reduced to increase the proportion of the second liquid in the confluence channel, pushing the laminar interface toward the sidewall of the confluence channel on the first branch channel side. Similarly, during the flow rate adjustment process, the position of the laminar interface is continuously monitored until the difference between the updated current interface position information and the target interface position information is less than or equal to a preset difference, at which point the adjustment stops. In this way, by determining the interface offset direction and adjusting the flow rate, the position of the laminar interface is adjusted. This achieves precise control of the ratio of the first and second liquids without requiring precise monitoring and control of the specific flow rate, ensuring that the two liquids in the confluence channel meet preset requirements. This ensures control precision and detection accuracy while reducing the difficulty of control.

[0152] Based on some or all of the above embodiments, in other embodiments, a conveying device such as a conveying pump is easily installed in the second branch channel to realize the flow rate control and detection of the second liquid. Accordingly, in S203, at least one of the flow rates of the first liquid and the second liquid is adjusted until the updated current interface position information matches the target interface position information, referring to... Figure 8 The method also includes the following S301-S305:

[0153] S301: Obtain the current flow rate of the second liquid;

[0154] Specifically, the current flow rate of the second liquid can be determined based on the operating parameters of the flow regulating device used to transport the second liquid, such as the relationship between the rotational speed of the delivery pump and the flow rate of the second liquid. The relationship between rotational speed and flow rate can be determined based on existing technology, and this application does not impose any limitations on it. The current flow rate of the second liquid can be the real-time flow rate of the reference solution at the current detection node. The flow rate of the second liquid at different detection nodes can be the same or different.

[0155] S303: Determine the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid;

[0156] Understandably, given the current flow rate of the second liquid and the flow rate ratio of the first liquid to the second liquid, the current flow rate of the first liquid can be determined.

[0157] In this application, the second liquid and the first liquid form a laminar flow in a confluence channel. Correspondingly, the surfaces where the second liquid and the first liquid come into contact can form an interface. The interface position information of the laminar flow in the confluence channel can characterize the velocity ratio information of the first liquid and the second liquid. Therefore, by detecting the interface of the laminar flow in the confluence channel, the interface position information can be obtained, and the velocity ratio of the first liquid and the second liquid can be determined based on the interface position information. Given the current velocity of the second liquid, the current velocity of the first liquid can be determined based on the current velocity and the velocity ratio. The liquid velocity detection method in this application can measure the velocity of the first liquid in the laminar flow separately, thereby improving the efficiency and accuracy of the first liquid velocity detection.

[0158] Specifically, after determining the current flow rate ratio and the current flow rate of the second liquid, the current flow rate of the first liquid can be the product of the current flow rate ratio and the current flow rate of the second liquid. Thus, by pre-setting a second pre-defined correspondence and monitoring the laminar flow interface position, the flow rate of the first liquid is determined, facilitating first liquid flow rate monitoring and subsequent consumption calculations.

[0159] Accordingly, adjusting at least one of the flow rates of the first liquid and the second liquid in S203 until the updated current interface position information matches the target interface position information includes:

[0160] S2034: Based on the second preset correspondence, determine the current flow rate ratio corresponding to the current interface position information and the target flow rate ratio corresponding to the target interface position information. The second preset correspondence is used to characterize the correspondence between the preset flow rate ratio and multiple interface position information of the laminar flow distribution interface in the converging flow channel.

[0161] Specifically, a second preset correspondence between interface position information and a preset flow rate ratio can be obtained based on historical experimental data. Therefore, after detecting the current interface position information, the current flow rate ratio corresponding to the currently detected interface position information can be determined based on this second preset correspondence. The flow rate ratio is the ratio of the flow rate of the first liquid to the flow rate of the second liquid. Specifically, the channel size parameters used to determine the correspondence between the interface position information and the flow rate ratio can be the same as or different from the channel size parameters used when detecting the liquid flow rate.

[0162] Combining the aforementioned methods for determining interface location information, in some cases, edge detection is performed on the channel walls and interface of the confluence flow path. Based on the edge detection results, the vertical distance from the two side pipe walls to the interface is determined. This vertical distance can characterize the proportion of the two liquids in the confluence flow path, and thus characterize the corresponding flow velocity information. Therefore, determining the interface location information based on distance can characterize the liquid proportion information. There is a correspondence between the liquid proportion information and the flow velocity, thereby improving the accuracy of subsequent flow velocity determination. In other cases, the correspondence between the current interface location information and the flow velocity ratio can be a correspondence between slope and flow velocity ratio. After determining the current interface location information, the corresponding flow velocity ratio can be determined. Thus, at the initial moment when the second liquid and the first liquid meet, the laminar flow is not stable, and the laminar interface may be a slope. By determining the slope of this laminar interface, and based on the correspondence between the slope and the flow velocity ratio, the flow velocity ratio at the initial moment of the second liquid and the first liquid meeting can be determined, thereby determining the flow velocity of the first liquid. This achieves the detection of the first liquid flow velocity at the beginning of the meeting.

[0163] Specifically, the target flow rate ratio refers to the flow rate ratio between the first liquid and the second liquid required to maintain the laminar interface at the desired position corresponding to the target interface position information; the current flow rate ratio refers to the flow rate ratio between the first liquid and the second liquid required to maintain the interface at the current position.

[0164] S2035: Based on the current flow rate of the second liquid and the current flow rate of the first liquid, adjust the flow rate of at least one of the first liquid and the second liquid until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information matches the target interface position information.

[0165] Specifically, the target flow rates of the second liquid and / or the first liquid can be calculated using the target flow rate ratio. Then, by adjusting the operating parameters of the flow rate regulating device, the second liquid and / or the first liquid can be adjusted to their corresponding target flow rates, achieving precise control of the flow rate ratio. This allows the laminar interface to reach the desired position. Thus, by pre-calibrating the second preset correspondence, it is not necessary to monitor the current interface position information of the laminar interface in real time during the flow rate adjustment process. Once the target flow rate ratio is reached, the position of the laminar interface can be controlled, avoiding the control deviation introduced by the unstable position of the laminar interface and reducing the resource consumption of laminar interface position analysis.

[0166] Understandably, if the updated current interface position information does not match the target interface position information after the target flow rate ratio is reached and stabilized for a certain period of time, it indicates that there is a deviation in the calibrated second preset correspondence. The updated current interface position information can be adjusted to match the target interface position information through the methods described in S2031-S2033 above, and the target flow rate ratio corresponding to the target interface position information in the second preset correspondence is updated based on the flow rate ratio in the matching state, so as to achieve calibration relationship correction.

[0167] Based on some or all of the above embodiments, in some embodiments, during the flow rate adjustment process, the flow rate of either the first liquid or the second liquid is adjusted while the flow rate of the other is kept stable to regulate the flow rate ratio, thereby reducing the complexity of the regulation. Specifically, the rotational speed of the delivery pump on the first branch channel or the second branch channel can be adjusted.

[0168] In some embodiments, the flow rate ratio is controlled by stabilizing the flow rate of the first liquid in the first branch channel and adjusting the flow rate of the second liquid in the second branch channel to regulate the flow rate ratio. Thus, by decreasing or increasing the flow rate of the second liquid, the flow rate ratio is increased or decreased, causing the laminar interface position to move to the desired location. This can be applied to scenarios where it is difficult to control the flow rate of the first liquid, reducing the device layout requirements of the first liquid pipeline. For example, in blood monitoring scenarios (such as ECMO blood monitoring), the first branch channel is connected to a branch of the blood circulation pipeline, making blood flow rate difficult to control and adjust. The above method is used to adjust the flow rate ratio to achieve laminar interface position adjustment.

[0169] Furthermore, given that the flow rate of the first liquid is finely adjustable, if the flow rate of the second liquid in the second branch channel reaches its upper or lower limit, the flow rate of the second liquid in the second branch channel is stabilized, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio. Thus, when the flow rate of the second liquid reaches its upper or lower limit, such as reaching the upper limit for laminar flow formation or the lower limit for adjustable flow rate, the flow rate ratio is adjusted by regulating the flow rate of the first liquid, thereby improving the flexibility and reliability of interface control.

[0170] In other embodiments, the flow rate ratio is controlled as follows: the flow rate of the second liquid in the second branch channel is stabilized, and the flow rate of the first liquid in the first branch channel is adjusted to control the flow rate ratio; if the flow rate of the first liquid in the first branch channel reaches the upper or lower limit of the first liquid flow rate, the flow rate of the first liquid in the first branch channel is stabilized, and the flow rate of the second liquid in the second branch channel is adjusted to control the flow rate ratio. Thus, by prioritizing the control of the first liquid flow rate for flow rate ratio adjustment, the stability of the amount of second liquid added and the content of the first liquid are ensured, thereby ensuring measurement accuracy.

[0171] Based on some or all of the above embodiments, in other embodiments, the flow rate ratio is controlled using the following S401-S403:

[0172] S401: If it is necessary to reduce the flow rate ratio between the first liquid and the second liquid, and control the flow rate of the second liquid in the second branch channel to stabilize, reduce the flow rate of the first liquid in the first branch channel.

[0173] S403: If it is necessary to increase the flow rate ratio between the first liquid and the second liquid, control the flow rate of the first liquid in the first branch channel to stabilize, and decrease the flow rate of the second liquid in the second branch channel.

[0174] This ensures that the flow rate of the first fluid is adjusted to be lower rather than higher, avoiding excessive loss of the first fluid, which is especially suitable for real-time blood monitoring scenarios.

[0175] Based on some or all of the above embodiments, in some embodiments, S203 further includes: under the first flow velocity constraint condition, adjusting at least one of the flow velocity of the first liquid and the flow velocity of the second liquid until the updated current interface position information matches the target interface position information; the first flow velocity constraint condition is used to control the flow velocity of the first liquid within a first flow velocity range, the first flow velocity range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel. Specifically, the first flow velocity range is determined based on the preset Reynolds number range corresponding to the first liquid, the fluid density, the viscosity coefficient, the characteristic linear dimension of the first branch channel, and the characteristic linear dimension of the confluence channel, the preset Reynolds number range being the Reynolds number range that can form stable laminar flow at the microfluidic scale. It can be understood that the characteristic linear dimension of the first branch channel and the characteristic linear dimension of the confluence channel may be different, and therefore the flow velocity range calculated according to the Reynolds number formula will also be different. The first flow velocity range is the intersection between the flow velocity range calculated based on the characteristic linear dimension of the first branch channel and the flow velocity range calculated based on the characteristic linear dimension of the confluence channel. Presetting a flow rate range ensures that the first liquid forms a stable laminar flow, which is beneficial for laminar flow status monitoring and flow rate regulation.

[0176] In some embodiments, the first velocity constraint is also used to control the flow velocity of the second liquid within a second velocity range. This second velocity range is the range of fluid velocities required to maintain the laminar flow state of the second liquid in the second branch channel and the confluence channel. Specifically, the second velocity range is determined based on a preset Reynolds number range corresponding to the second liquid, the fluid density, viscosity coefficient, the characteristic linear dimension of the second branch channel, and the characteristic linear dimension of the confluence channel. The preset Reynolds number range corresponding to the second liquid can be the same as or different from the preset Reynolds number range corresponding to the first liquid. Understandably, the characteristic linear dimension of the second branch channel and the characteristic linear dimension of the confluence channel may be different. The second velocity range is the intersection of the velocity range calculated based on the characteristic linear dimension of the second branch channel and the velocity range calculated based on the characteristic linear dimension of the confluence channel. By setting the velocity range, a stable laminar flow is ensured for the second liquid, forming a stable laminar interface with the first liquid, which is beneficial for laminar flow state monitoring and velocity regulation.

[0177] Understandably, the method of this application can be applied to blood detection scenarios. Accordingly, when the first liquid is blood, S203 further includes: under the second flow rate constraint condition, adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information; the second flow rate constraint condition is used to control the flow rate of the first liquid within a third flow rate range, the third flow rate range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the converging channel, and to prevent the first liquid from forming a coagulation state, so as to ensure that the blood maintains a laminar flow state and does not coagulate in the microchannel.

[0178] Specifically, when setting a first flow velocity range and a second flow velocity range, in the aforementioned flow velocity ratio control process, if the flow velocity of the first liquid in the first branch channel is kept stable and the flow velocity of the second liquid in the second branch channel is adjusted, when the flow velocity of the second liquid reaches the boundary value of the second flow velocity range, the position of the laminar interface is still not at the desired position, then the flow velocity of the second liquid is kept stable and the flow velocity of the first liquid is adjusted. If the flow rate ratio adjustment method of S401-S403 is adopted, during the process of controlling the flow rate of the second liquid in the second branch channel to be stable and reducing the flow rate of the first liquid in the first branch channel, if the flow rate of the first liquid reaches the boundary value of the first flow rate range, but the position of the laminar interface has not reached the desired position, then the method is switched to keep the flow rate of the first liquid stable and adjust the flow rate of the second liquid; during the process of controlling the flow rate of the first liquid in the first branch channel to be stable and reducing the flow rate of the second liquid in the second branch channel, if the flow rate of the second liquid reaches the boundary value of the second flow rate range, but the position of the laminar interface has not reached the desired position, then the method is switched to keep the flow rate of the second liquid stable and adjust the flow rate of the first liquid.

[0179] Based on some or all of the above embodiments, in some embodiments, the microfluidic device further includes a first detection device and a mixing channel communicating with the confluence channel. The mixing channel is used to mix the target fluid so that the reaction solution mixes with the first liquid and undergoes a specific reaction to form a target mixture. That is, the target fluid formed by the first liquid and the second liquid in the confluence channel flows into the mixing channel and is mixed uniformly in the mixing channel. The first detection device is used to detect markers in the mixture. Understandably, multiple first detection devices can be provided to detect at least one marker in the mixture. Specifically, the first detection device can be a contact sensor or a non-contact sensor, and may include, but is not limited to, electrochemical sensors or photoelectric sensors. Preferably, the microfluidic device with the mixing channel and the first detection device can adopt a Y-shaped channel design. In one embodiment, refer to Figure 5 The microfluidic chip can also be equipped with an auxiliary channel, which has an inlet 045. The auxiliary channel and the output of the mixing channel are connected. After the mixed liquid enters the detection section 048 of the auxiliary channel, it is divided by the slitting liquid within it, forming droplets. These droplets pass through the detection area of ​​the first detection device to achieve specific detection. The slitting solution injected into the auxiliary channel can be an oily liquid. The microfluidic chip shown in the figure can be used in blood monitoring scenarios such as anti-Xa detection.

[0180] Accordingly, in the current working mode of mixed liquid detection mode, the second liquid is a reaction solution that can specifically react with the first liquid; during the mixing process of the first liquid and the second liquid in the mixing channel, a specific reaction occurs to generate specific reactants, thereby forming the target mixed liquid.

[0181] Accordingly, in S203, at least one of the flow rates of the first liquid and the second liquid is adjusted until the updated current interface position information matches the target interface position information, so that the target fluid is formed in the confluence channel, and then reference is made. Figure 9 The method also includes the following S501-S503:

[0182] S501: The target mixture is detected by the first detection device to obtain the first detection parameter data, which is used to indicate the content information of the target substance in the target mixture;

[0183] S503: Generate detection results based on the first detection parameter data.

[0184] Specifically, the target substance is the substance to be detected by the first detection device, and may include at least one biomarker. A corresponding detection result is generated based on the first detection parameter data. For example, in anti-Xa detection, the first liquid is blood containing heparin, and the second liquid may contain anticoagulants such as anticoagulant enzyme (AT) that can specifically react with heparin, and may contain an excess of active Xa factor. The specific reactant is the combination of heparin and anticoagulant enzyme (AT) to form a complex, which neutralizes the active Xa factor. The target substance is the active Xa factor. The first detection parameter data characterizes the activity of the remaining active Xa factor in the target mixture, and is inversely proportional to the effective concentration of heparin in the blood. Based on this inverse relationship, the effective concentration of heparin is determined, and the detection result is obtained. Specifically, the first detection parameter data can be a signal of the specific reaction, such as an electrical signal (including current, voltage, or impedance), an optical signal, etc. In anti-Xa detection applications, the first detection device can be, for example, an electrochemical sensor for anticoagulation in point-of-care testing (POCT), or a sensor capable of detecting droplet color to determine the concentration of anti-Xa factor. The correspondence between color and anti-Xa factor concentration can be determined according to the specific kit requirements.

[0185] In this way, by regulating the flow rates of the first liquid and the reaction solution, the position of the laminar flow interface can be adjusted, and the mixing ratio between the two can be precisely controlled based on the position of the laminar flow interface, reducing the difficulty of flow rate and ratio control and improving detection accuracy and efficiency.

[0186] Understandably, in mixed liquid detection mode, non-contact sensors, such as optical sensors, are preferred as the first detection device, or contact sensors are used but the first liquid is discarded and not returned to the body (such as using a Y-shaped flow channel) to ensure that no risk of contamination or blood clotting is introduced to the greatest extent possible, which is beneficial for the replacement and maintenance of consumables.

[0187] As mentioned earlier, the mixing ratio between the first and second liquids can be adjusted by regulating the position of the laminar interface. Accordingly, in the current operating mode of mixture detection, the target interface position information is determined based on the preset mixing ratio between the first and second liquids. By calibrating the correspondence between various proportions of the first liquid in the target mixture and the interface position information of the laminar interface under each proportion, the interface position information corresponding to the preset mixing ratio can be determined as the target interface position information. In this way, establishing a correspondence between the mixing ratio and the interface position information is beneficial for laminar interface control and the calculation of parameters such as the dilution factor of the first liquid in the mixture.

[0188] Furthermore, the first liquid is blood; and in cases where the target substance includes an anticoagulant, the first detection parameter data includes detection data used to indicate the anticoagulant level of the blood.

[0189] In one embodiment, when the first liquid is blood and the target substance includes an anticoagulant, the reaction solution contains a pre-defined reactant capable of specifically reacting with the anticoagulant in the blood, such as the aforementioned anticoagulant enzyme. When the target substance is anti-Xa factor, the content information of the target substance indicated by the first detection parameter data can characterize the activity of Xa factor. The pre-defined mixing ratio between the first and second liquids is 1:1, and correspondingly, the desired position corresponding to the target interface position information is the center of the confluence channel radially. Typically, when the pre-defined mixing ratio is 1:1, adjusting the flow rate ratio of the first and second liquids to 1:1 allows the laminar interface to reach the desired position, horizontally centered in the confluence channel, specifically the central axis of the confluence channel. It is understood that the above method is not limited to anti-Xa detection but can also be used in other detection scenarios requiring the introduction of a reaction solution, such as protein biomarker detection.

[0190] In some embodiments, while obtaining the first detection parameter data, it is also necessary to determine the dilution factor of the first liquid in the target mixture in order to combine it with the generated detection results. For example, in some anti-Xa detections, this dilution factor is used to assist in calculating the Xa factor concentration. Accordingly, refer to Figure 10 The first liquid dilution factor is obtained using the following steps S601-S605:

[0191] S601: Obtain the interface position information corresponding to the target mixture;

[0192] S603: Determine the actual mixing ratio between the first liquid and the second liquid based on the interface position information corresponding to the target mixture;

[0193] S605: Determine the first liquid dilution factor corresponding to the target mixture based on the actual mixing ratio.

[0194] Accordingly, the generation of detection results based on the first detection parameter data includes:

[0195] The test results are generated based on the first detection parameter data and the first liquid dilution factor.

[0196] Specifically, the interface position information corresponding to the target mixture refers to the interface position information of the laminar flow interface in the confluence channel when the first detection parameter data is obtained. Based on the correspondence between the interface position information and the mixing ratio, the actual mixing ratio can be obtained, and then the proportion of the first liquid in the target mixture can be calculated to determine its dilution factor. The dilution factor of the first liquid can be used for auxiliary calculation of the content of the target substance to generate the detection result in combination with the first detection parameter data.

[0197] Based on some or all of the above embodiments, in other embodiments, the microfluidic device further includes a second detection device disposed on the confluence channel, the second liquid being a reference solution (BLS solution); the microfluidic device may adopt an X-type or Y-type flow channel design, and the second detection device is used to detect the liquid flowing through the confluence channel. Specifically, the reference solution may be a liquid used to assist in the measurement of the first liquid; the reference solution has stable and known components, for example, the reference solution contains a target marker, and the concentration of the target marker is known, such as the known pH value of the reference solution, etc. The reference solution can be used to rinse the second detection device, removing any residues that may contaminate the second detection device, and calibrating the detection device while cleaning it. Specifically, the second detection device may be a contact sensor or a non-contact sensor, and may include, but is not limited to, electrochemical sensors or photoelectric sensors. The operating modes of the second detection device include at least a cleaning mode and a measurement mode. The cleaning mode refers to cleaning the sensing area of ​​the second detection device with the reference solution, and the measurement mode refers to detecting the first liquid based on the sensing area of ​​the second detection device. It is understood that multiple second detection devices may be disposed at the confluence channel.

[0198] Accordingly, in the current working mode of cleaning the second detection device, the desired position corresponding to the target interface position information is where the laminar flow interface deviates from the first branch channel side and the reference solution covers the detection area of ​​the second detection device. Specifically, to ensure the reference solution covers the detection area, the flow rate of the second liquid can be adjusted to be higher than that of the first liquid, so that the second liquid occupies more volume in the confluence channel until the updated current interface position information is detected to reach the desired position, or between the desired position and the sidewall of the confluence channel on the first branch channel side, so that the second liquid at least covers the detection area, thus cleaning the second detection device. Understandably, in some cases, a target flow rate ratio corresponding to the cleaning mode can also be preset. In response to the cleaning mode control command, the flow rates of the first liquid and / or the second liquid can be adjusted to reach the target flow rate ratio, so that the updated interface position information matches the target interface position information corresponding to the cleaning mode, thereby achieving cleaning.

[0199] In the current operating mode, which is a measurement mode based on the second detection device, the desired position corresponding to the target interface position information is where the laminar flow interface deviates towards the second branch channel side and the first liquid covers the detection area of ​​the second detection device. Specifically, to ensure the first liquid covers the detection area, the flow rate of the first liquid can be adjusted to be higher than that of the second liquid, so that the first liquid occupies more volume in the confluence channel until the updated current interface position information is detected to reach the desired position, or between the desired position and the sidewall of the confluence channel on the second branch channel side, so that the first liquid at least covers the detection area, thus meeting the detection conditions. Understandably, in some cases, a target flow rate ratio corresponding to the measurement mode can also be preset. In response to the measurement mode control command, the flow rates of the first liquid and / or the second liquid can be adjusted to reach the target flow rate ratio, so that the updated interface position information matches the target interface position information corresponding to the measurement mode, thereby achieving measurement. By detecting the laminar interface position and adjusting the flow rate based on the desired position corresponding to the target interface position information, the first liquid and the reference solution can alternately cover the detection element, thereby achieving efficient switching between cleaning mode and measurement mode. It is easy to operate and can be applied to microfluidic devices with different flow channel types, shapes and sizes.

[0200] In some embodiments, in the cleaning mode, after S203, the method further includes the following S701-S703:

[0201] S701: Acquire preset parameter data of the reference solution and second detection parameter data of the second detection device. The second detection parameter data is used to indicate the content information of the target marker in the reference solution flowing through the second detection device.

[0202] S703: If the preset parameter data is consistent with the second detection parameter data, the cleaning of the second detection equipment is confirmed to be complete.

[0203] In some embodiments, the second detection device can be used to detect parameters of the liquids in the confluence channel. That is, the second detection device can be used to detect parameters of the reference solution in the confluence channel, parameters of the first liquid in the confluence channel, and parameters of the confluence liquids in the confluence channel. The reference solution has stable and known components; for example, its pH value is known, or the reference solution contains a target marker, and the concentration of the target marker is known.

[0204] Specifically, the target marker is the substance to be detected by the second detection device, contained in the first liquid and the reference solution. Specifically, the reference solution has preset parameter data characterizing the solution properties. The preset parameter data refers to the target parameters that the second detection device should obtain when detecting the target marker in the reference solution. The preset parameter data can be the pH value of the reference solution, or the concentration of the target marker contained in the reference solution, etc. Understandably, the content of the target marker in the reference solution is known, and correspondingly, the preset parameter data is known. If the second detection parameter data is consistent with the preset parameter data, it indicates that the cleaning effect has been achieved. Preferably, if the preset parameter data is consistent with the second detection parameter data, and the second detection parameter data remains stable (e.g., the value fluctuates within a preset range over a certain period of time), then the cleaning standard is determined to have been met. After cleaning, the device can switch to measurement mode to perform first liquid detection. Thus, mode switching is achieved by combining parameter measurement and laminar flow interface position adjustment.

[0205] If the preset parameter data is inconsistent with the second detection parameter data, but the current interface position meets the expected position in the cleaning mode, that is, the laminar flow interface is deviated towards the first branch channel side and the reference solution covers the detection area of ​​the second detection device, the second detection device can be calibrated according to the second detection parameter data.

[0206] Specifically, when the second detection device can detect a liquid parameter, such as the pH value of the liquid, the preset parameter data of the reference solution can be the preset pH value, and the second detection parameter data is the detected pH value. When the detected pH value is consistent with the preset pH value, it can be said that the cleaning is achieved after the reference solution completely covers the sensing area of ​​the detection device. Accordingly, the updated interface position information in the confluence channel under the cleaning mode can also be determined at this time.

[0207] When the second detection device can detect a liquid parameter, such as the concentration of a target marker, the preset parameter data can be the preset concentration of the target marker in the reference solution, and the second detection parameter data is the detection concentration. When the detection concentration is consistent with the preset concentration, it can be said that the reference solution completely covers the sensing area of ​​the detection device to achieve cleaning of the detection device. Correspondingly, the updated interface position information in the confluence channel under the cleaning mode can also be determined at this time.

[0208] When the second detection device can detect multiple liquid parameters, such as the pH value or the concentration of the target marker, the preset parameter data of the reference solution can include the preset pH value and the preset concentration of the target marker in the reference solution. The second detection parameter data includes the detected pH value and the detected concentration. Thus, when the detected pH value is consistent with the preset pH value and the detected concentration is consistent with the preset concentration, it can be concluded that the reference solution completely covers the sensing area of ​​the detection device and the device is cleaned. Accordingly, the updated interface position information in the confluence channel under the cleaning mode can also be determined at this time.

[0209] Therefore, if the second detection parameter data detected by the detection device is consistent with the preset parameter data of the reference solution, it indicates that the reference solution cleans the detection device while completely covering the sensing area of ​​the detection device. This allows for the determination of the updated interface position information of the laminar flow interface in the confluence channel under the cleaning mode, which is useful for position verification and calibration.

[0210] Furthermore, if the second detection parameter data is inconsistent with the preset parameter data, the operating parameters of the flow regulating device can be adjusted to increase the flow rate of the reference solution in the confluence channel.

[0211] If the second detection parameter data is inconsistent with the preset parameter data when cleaning the detection equipment, it indicates that the reference solution has not completely covered the sensing area of ​​the detection equipment or has not been cleaned completely. In this case, the flow rate of the reference solution can be increased to ensure that the reference solution can completely cover the sensing area or continue cleaning.

[0212] When the detection device can detect a liquid parameter, such as the pH value of the solution, the preset parameter data of the reference solution can be the preset pH value, and the second detection parameter data can be the detected pH value. If the detected pH value is inconsistent with the preset pH value, it can be said that the reference solution has not completely covered the sensing area of ​​the detection device or has not been cleaned. In this case, the flow rate of the reference solution can be adjusted so that the reference solution completely covers the sensing area or continues to clean.

[0213] When the detection device can detect a liquid parameter, such as the concentration of a target marker, the reference solution can contain the target marker. The preset parameter data can be the preset concentration of the target marker in the reference solution, and the second detection parameter data is the detection concentration. If the detection concentration is inconsistent with the preset concentration, it indicates that the reference solution has not completely covered the sensing area or has not been cleaned. In this case, the flow rate of the reference solution can be adjusted to ensure that the reference solution completely covers the sensing area or continues to clean.

[0214] When the detection device can detect multiple liquid parameters, such as the pH value of the solution or the concentration of the target marker, the preset parameter data of the reference solution can include the preset pH value and the preset concentration of the target marker in the reference solution. The second detection parameter data includes the detected pH value and the detected concentration. Therefore, if the detected pH value is inconsistent with the preset pH value and / or the detected concentration is inconsistent with the preset concentration, it can be indicated that the reference solution has not completely covered the sensing area or has not been cleaned completely. At this time, the flow rate of the reference solution can be adjusted so that the reference solution completely covers the sensing area or continues to clean.

[0215] Therefore, when the second detection parameter data is inconsistent with the preset parameter data, the operating parameters of the flow regulating device corresponding to the reference solution can be adjusted to increase the flow rate of the reference solution, so as to achieve complete coverage or continuous cleaning of the sensing area of ​​the detection equipment by the reference solution. This enables effective cleaning of the detection equipment, and when adjusted to ensure that the reference solution completely covers the sensing area, it is convenient to determine the updated interface position information corresponding to the cleaning mode.

[0216] The target interface position information corresponding to the cleaning mode can be understood as follows: when cleaning the detection equipment, the interface position information of the interface between the reference solution and the first liquid can be adjusted to the corresponding position, thereby achieving complete coverage of the sensing area of ​​the detection equipment by the reference solution, and thus realizing cleaning of the detection equipment based on the reference solution. Having determined the target interface position information corresponding to the cleaning mode, this information determines the target flow rate ratio between the first liquid and the reference solution in the cleaning mode; subsequently, based on the flow rate of the reference solution and the flow rate ratio in the cleaning mode, the flow rate of the first liquid in the cleaning mode is determined.

[0217] In some embodiments, when entering the cleaning mode, the control host may respond to the cleaning mode control command to control the reference solution to flow at a first preset flow rate and control the first liquid to flow at a second preset flow rate; the first preset flow rate and the second preset flow rate are determined based on the target flow rate ratio in the cleaning mode, the current flow rate of the reference solution and the current flow rate of the first liquid.

[0218] In this embodiment, the reference solution can be transported via a corresponding flow regulating device, and the first liquid can also be transported via a corresponding flow regulating device. In order to ensure that the interface position information of the two liquids is consistent with the target interface position information corresponding to the cleaning mode, the flow rate ratio of the two liquids should satisfy the target flow rate ratio corresponding to the cleaning mode. For example, the first flow rate ratio of the first preset flow rate to the second preset flow rate is equal to the second flow rate ratio of the flow rate of the first liquid to the flow rate of the reference solution. When the first flow rate ratio and the second flow rate ratio are equal, the first preset flow rate can be the flow rate of the reference solution * n, and the second preset flow rate can be the flow rate of the first liquid * n, where n is a real number greater than 0, such as n = 1, n = 2, etc.

[0219] By determining the flow rate relationship between the reference solution and the first liquid under the cleaning mode based on the interface position information corresponding to the cleaning mode, it is convenient to directly control the specific flow rates of the reference solution and the first liquid based on the flow rate relationship under the cleaning mode when the detection equipment needs to be cleaned. This enables the reference solution to effectively cover and continuously clean the detection equipment without having to adjust the flow rates of the reference solution and the first liquid multiple times to meet the cleaning conditions. This achieves precise flow rate control under the cleaning mode and improves cleaning efficiency.

[0220] Furthermore, in the cleaning mode, after S203, the method may further include the following S705: if the preset parameter data is inconsistent with the second detection parameter data, and the second detection parameter data remains stable, adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid; if the preset parameter data is detected to be consistent with the second detection parameter data, determine that the second detection device has completed cleaning. In the case of inconsistency, the aforementioned flow rate ratio will be reduced to further increase the coverage area of ​​the second liquid; if subsequent detections show that the preset parameter data is consistent with the second detection parameter data, it indicates that cleaning is complete.

[0221] Specifically, if the second detection parameter data is detected to be stable but inconsistent with the previous data, it indicates the possibility that the reference solution has not covered the sensing area of ​​the second detection device, and the target interface position information corresponding to the cleaning mode may be deviated. Accordingly, the flow rates of the first liquid and / or the second liquid are adjusted based on the aforementioned flow rate adjustment method to reduce the flow rate ratio and increase the volume percentage of the second liquid in the confluence channel, thereby calibrating the position of the laminar flow interface and ensuring continued cleaning. If cleaning is completed, a switch to the measurement mode can be performed. Thus, by combining parameter detection and laminar flow interface position detection, calibration data is calibrated, improving detection accuracy. Preferably, after the flow rate ratio is adjusted to match the preset parameter data and the updated second detection parameter data, the updated second detection parameter data is continuously monitored. If it remains stable, the cleaning standard is determined to have been met. Understandably, if the preset parameter data detected after the set time is still inconsistent with the second detection parameter data, there is a risk of failure of the second detection device. This would cause the second detection parameter data to remain consistent with the preset parameter data of the reference solution even after the reference solution has completely covered the second detection device and been cleaned for a certain period of time, thereby generating an alarm message for the second detection device.

[0222] Furthermore, after the preset parameter data and the updated second detection parameter data are consistent, the method may further include the following steps S707-S709:

[0223] S707: When the preset parameter data and the updated second detection parameter data are consistent, obtain the updated interface position information;

[0224] S709: Calibrate the target interface position information corresponding to the current working mode based on the updated interface position information.

[0225] Specifically, the updated interface position information of the confluence channels in the consistent state is replaced with the target interface position information corresponding to the cleaning mode, so as to realize the calibration of the target interface position information corresponding to the cleaning mode and ensure the cleaning effect and detection accuracy.

[0226] In some embodiments, in measurement mode, after S203, the method further includes the following S801-S803:

[0227] S801: Obtain the third detection parameter data of the second detection device. The third detection parameter data is used to indicate the content information of the target marker in the first liquid flowing through the second detection device.

[0228] S803: If the third detection parameter data remains stable within a preset time period, generate a detection result based on the third detection parameter data.

[0229] Specifically, by adjusting the flow rate to increase the flow rate ratio, if the third detection parameter data remains stable within a certain time period, it indicates that the third detection parameter data can be used to generate detection results. For example, if the target marker is oxygen, the detection result can be the oxygen content in the blood. Combining parameter detection and laminar flow interface position detection enables the determination of markers in the first liquid, improving detection accuracy. Understandably, the measurement mode needs to be triggered based on the completion of cleaning according to the cleaning standard. That is, ensuring that cleaning is completed in the cleaning mode, the flow rate of the first liquid and / or the second liquid is adjusted in response to the measurement mode control command, the flow rate ratio is increased, the measurement mode is switched, and S801 is executed for detection.

[0230] When the second detection device can detect a liquid parameter, for example, if the target value for measuring the first liquid is pH, and the second detection device can detect the pH value of the liquid, the preset parameter data of the reference solution can be the preset pH value, and the third detection parameter data is the detected pH value. If the detected pH value remains stable over a period of time, it indicates that the measurement has reached a steady state, which means that the first liquid has completely covered the second detection device, and the measurement of the first liquid can be realized. At this time, the updated interface position information in the confluence channel under the measurement mode can also be determined.

[0231] When the second detection device can detect a liquid parameter, for example, the measurement target of the first liquid is the concentration of a target marker, the second detection device can detect the concentration of the target marker, and the reference solution can contain the target marker. The preset parameter data can be the preset concentration of the target marker in the reference solution, and the third detection parameter data is the detection concentration. When the detection concentration remains unchanged for a period of time, it indicates that the measurement has reached a steady state, which means that the first liquid has completely covered the second detection device, and the measurement of the first liquid can be realized. At this time, the updated interface position information in the confluence channel under the measurement mode can also be determined.

[0232] When the second detection device can detect multiple liquid parameters, specifically including both measurement parameters and reference parameters, the measurement parameters can be used as the target for measuring the first liquid, while the reference parameters are used to determine whether the second detection device is completely covered by the first liquid. The reference parameters for the first liquid are known, while the measurement parameters need to be obtained through measurement by the second detection device. For example, if the second detection device can detect the pH value of the liquid or the concentration of a target marker, the concentration of the target marker can be used as the measurement parameter, and the pH value of the first liquid can be used as the reference parameter. Therefore, if the pH value detected by the second detection device matches the reference parameter of the first liquid, it indicates that the second detection device is completely covered by the first liquid, and measurement of the first liquid can be achieved. At this point, the updated interface position information in the confluence channel during the measurement mode can also be determined.

[0233] If the third detection parameter data detected by the second detection device remains stable for a period of time, it indicates that the first liquid has completely covered the second detection device, enabling the measurement of the first liquid. Furthermore, it can determine the updated interface position information of the laminar flow interface in the confluence channel under the measurement mode, so as to facilitate position verification and preset interface position calibration, etc.

[0234] Furthermore, in the measurement mode, after S203, the method may also include the following S805-S807:

[0235] S805: If the third detection parameter data is unstable within a preset time period, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid until the updated third detection parameter data remains stable within the preset time period.

[0236] S807: Generate detection results based on updated third detection parameter data.

[0237] Specifically, if the third detection parameter data is unstable within a preset time period, there is a possibility that the current first liquid may not cover the sensing area of ​​the second detection device. Accordingly, the volume percentage of the first liquid in the confluence channel is increased, pushing the laminar flow interface position towards the sidewall of the confluence channel on the second branch channel side, ensuring that the first liquid can cover the sensing area. If the updated third detection parameter data remains stable for the preset time period, the updated third detection parameter data is determined to be accurate, and a detection result is generated. In this way, by combining laminar flow interface control with verification of questionable third detection parameter data, the accuracy of detection is improved.

[0238] Understandably, the content of the substance to be detected in the first liquid may change. The preset duration can be set based on actual needs to ensure accurate detection of the substance content in the first liquid while avoiding the omission of objective changes in the substance content.

[0239] Understandably, in cleaning or measurement mode, after S203, the interface position information of the laminar flow interface can be monitored to detect whether the current interface position information matches the target interface position information of the current working mode. If they do not match, the laminar flow interface position is automatically calibrated through the aforementioned speed adjustment method to ensure the cleaning or detection effect.

[0240] Furthermore, if the third detection parameter data is unstable within a preset time period, the operating parameters of the corresponding flow regulating device are adjusted to reduce the flow rate of the reference solution in the confluence channel.

[0241] In one embodiment, when the second detection device is capable of detecting a liquid parameter, such as the pH value of the first liquid, and the second detection device is capable of detecting the pH value of the liquid, the preset parameter data of the reference solution can be the preset pH value, and the third detection parameter data is the detected pH value. If the detected pH value is unstable within a preset time period, it indicates that there is a possibility that the sensing area of ​​the second detection device exists or is covered by the reference solution. At this time, the flow rate of the reference solution can be adjusted, specifically by reducing the flow rate of the reference solution, so that the first liquid can completely cover the second detection device.

[0242] When the second detection device can detect a liquid parameter, for example, the measurement target of the first liquid is the concentration of a target marker, the second detection device can detect the concentration of the target marker, and the reference solution can contain the target marker. The preset parameter data can be the preset concentration of the target marker in the reference solution, and the third detection parameter data is the detection concentration. If the detection concentration is unstable within a preset time, it indicates that there is a possibility that the sensing area of ​​the second detection device exists or is covered by the reference solution. At this time, the flow rate of the reference solution can be adjusted, specifically by reducing the flow rate of the reference solution, so that the first liquid can completely cover the second detection device.

[0243] When the second detection device can detect multiple liquid parameters, specifically including both measurement parameters and reference parameters, the measurement parameters can be used as the target for measuring the first liquid, while the reference parameters are used as the basis for determining whether the second detection device is completely covered by the first liquid. The reference parameters for the first liquid are known, while the measurement parameters need to be measured by the second detection device. For example, if the second detection device can detect the pH value of the liquid or the concentration of a target marker, the concentration of the target marker can be used as the measurement parameter, and the pH value of the first liquid as the reference parameter. Therefore, if the pH value detected by the second detection device is unstable within a preset time period, it indicates the possibility that the sensing area of ​​the second detection device is present or covered by a reference solution. In this case, the flow rate of the reference solution can be adjusted, specifically by reducing the flow rate, so that the first liquid can completely cover the second detection device.

[0244] If the third detection parameter data detected by the second detection device is unstable within a preset time period, it indicates that the first liquid may not completely cover the second detection device. The operating parameters of the first flow regulating device corresponding to the reference solution can be adjusted to reduce the flow rate of the reference solution, so as to achieve complete coverage of the second detection device by the first liquid. This enables effective measurement of the first liquid and facilitates the determination of the updated interface position information corresponding to the measurement mode when the first liquid completely covers the second detection device.

[0245] The target interface position information corresponding to the measurement mode can be understood as follows: when it is necessary to measure the first liquid based on the second detection device, the interface position information of the interface between the reference solution and the first liquid can be adjusted to the corresponding position, thereby achieving complete coverage of the second detection device by the first liquid, and thus enabling measurement of the first liquid based on the second detection device. Having determined the target interface position information corresponding to the measurement mode, this information determines the target flow rate ratio between the first liquid and the reference solution in the measurement mode; subsequently, based on the flow rate of the reference solution and the flow rate ratio in the measurement mode, the flow rate of the first liquid in the measurement mode is determined.

[0246] Thus, when entering the measurement mode, the control host can respond to the measurement mode control command to control the reference solution to flow at a third preset flow rate and control the first liquid to flow at a fourth preset flow rate; the third preset flow rate and the fourth preset flow rate are determined based on the target flow rate ratio, the flow rate of the first liquid and the flow rate of the reference solution.

[0247] In this embodiment, the reference solution can be transported via a corresponding flow regulating device, and the first liquid can be transported via a corresponding flow regulating device. In order to ensure that the interface position information of the two liquids is consistent with the target interface position information corresponding to the measurement mode, the flow rate relationship of the two liquids should satisfy the flow rate relationship corresponding to the target interface position information of the measurement mode. For example, it can be the first flow rate ratio of the third preset flow rate to the fourth preset flow rate, which is equal to the second flow rate ratio of the flow rate of the first liquid to the flow rate of the reference solution. When the first flow rate ratio and the second flow rate ratio are equal, it can be the third preset flow rate = the first flow rate * m, the fourth preset flow rate = the second flow rate * m, where m is a real number greater than 0, such as m = 1, m = 2, etc.

[0248] By determining the flow rate relationship between the reference solution and the first liquid under the measurement mode based on the interface position information corresponding to the measurement mode, it is convenient to directly control the specific flow rates of the reference solution and the first liquid based on the flow rate relationship between the reference solution and the first liquid under the measurement mode when it is necessary to measure the first liquid. This enables the first liquid to cover the second detection device without having to adjust the flow rates of the reference solution and the first liquid multiple times to meet the measurement conditions. This achieves precise flow rate control under the measurement mode and improves measurement efficiency.

[0249] In a specific example, when measuring the first liquid using the second detection device, if the current third detection parameter data of the second detection device remains stable after a preset time, it indicates that a steady state of measurement has been reached, and the detection result of the first liquid is determined based on the current third detection parameter data.

[0250] Specifically, when the second detection device can detect one liquid parameter, such as pH value or the concentration of a target marker, the corresponding measurement result can be the pH value of the first liquid or the concentration of the target marker in the first liquid; when the second detection device can detect two liquid parameters, such as using the concentration of the target marker as the measurement parameter and the pH value of the first liquid as the reference parameter of the first liquid, the corresponding measurement result includes the currently measured pH value and the currently measured concentration, wherein the currently measured pH value should be consistent with the reference parameter of the first liquid.

[0251] By ensuring the stability of the current third detection parameter data, the measurement results for the first liquid are determined, thus improving the accuracy of the measurement.

[0252] In one embodiment, please refer to Figure 6 It shows a schematic diagram of laminar flow changes in the confluence channel under cleaning and measurement modes, such as Figure 6 As shown in (a), at the initial moment, the reference solution and the first liquid flow at the same velocity in the confluence channel; for example, the initial velocity could be 50 μL / h. Figure 6 As shown in (b), in cleaning mode, the flow rate of the reference solution can be increased until the reference solution occupies most of the confluence channel and covers the entire second detection device, thereby rinsing the residue in the second detection device. When the second detection parameter data of the second detection device is consistent with the preset parameter data of the reference solution, the cleaning of the second detection device is determined to be complete; Figure 6 As shown in (c), in measurement mode, the flow rate of the reference solution can be reduced and the flow rate of the first liquid can be increased until the first liquid covers the entire second detection device. Correspondingly, the third detection parameter data of the second detection device can be recorded, and the detection result of the first liquid can be determined based on the third detection parameter data.

[0253] As described above in this embodiment, the operating stages of a liquid parameter detection system in a microfluidic channel may include a cleaning stage and a measurement stage. The flow rate ratio may differ for channels of different sizes at different operating stages, thus establishing a correspondence between channel size, operating stage, and flow rate ratio. Given the channel size information, the flow rate ratio of that known channel at different operating stages can be determined. The method for determining the flow rate ratio of a microfluidic channel of each size at different operating stages includes:

[0254] For any size microfluidic channel, when cleaning the second detection device based on the microfluidic channel of any size, the second detection parameter data of the second detection device is obtained;

[0255] If the second detection parameter data is consistent with the preset parameter data, the interface of the laminar flow in the confluence channel is detected to obtain the interface position information;

[0256] Based on the interface location information, the flow rate ratio between the reference solution and the first liquid in a microfluidic channel of any size is determined.

[0257] The above method can be used to determine the flow rate ratio of microfluidic channels of each size during the cleaning stage.

[0258] For any size microfluidic channel, when measuring the first liquid based on the microfluidic channel of any size, the third detection parameter data of the second detection device is obtained;

[0259] If the third detection parameter data remains stable within a preset time, the interface of the laminar flow in the confluence channel is detected to obtain the interface position information.

[0260] Based on the interface location information, the flow rate ratio between the reference solution and the first liquid in a microfluidic channel of any size is determined.

[0261] The above method can be used to determine the flow rate ratio of microfluidic channels of each size during the measurement stage.

[0262] Based on the determination of the flow rate ratio of each size of microfluidic channel at different operating stages, the corresponding flow rate ratio can be determined based on the size of the currently used microfluidic channel and the operating stage, thereby controlling the flow regulating device to deliver liquid based on the flow rate ratio.

[0263] This application also provides a microfluidic-based fluid control device 800, applied to a microfluidic device. The microfluidic device is provided with a first branch channel, a second branch channel, and a converging channel, the converging channel being connected to the first branch channel and the second branch channel, respectively. Figure 11 As shown, Figure 12 The diagram shows a structural schematic of a microfluidic-based fluid control device according to an embodiment of this application. The device may include the following modules.

[0264] Interface monitoring module 10: used to monitor the current interface position information of the laminar flow interface in the confluence channel; the first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow, and the laminar flow interface is the interface between the first liquid and the second liquid in the confluence channel.

[0265] Speed ​​control module 20: If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, so as to form the target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar flow interface in the confluence channel under the current working mode.

[0266] In some embodiments, the method further includes a target location determination module for determining target interface location information, which may be specifically used to: determine the target interface location information corresponding to the current working mode based on a first preset correspondence relationship, wherein the first preset correspondence relationship is used to characterize the correspondence between multiple working modes of the microfluidic device and preset location information.

[0267] In some embodiments, the speed control module 20 may include:

[0268] The offset direction determination submodule is used to determine the offset direction of the laminar flow interface relative to the desired position based on the target interface position information and the current interface position information.

[0269] First adjustment submodule: If the offset direction is the laminar interface offset towards the first branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid, so that the laminar interface moves towards the second branch channel side until the updated current interface position information matches the target interface position information.

[0270] Furthermore, the speed control module 20 may also include:

[0271] The second adjustment submodule is used to adjust at least one of the flow rates of the first liquid and the second liquid if the offset direction is the laminar interface shifting towards the second branch channel side, so as to reduce the flow rate ratio between the first liquid and the second liquid, until the laminar interface moves towards the first branch channel side until the updated current interface position information matches the target interface position information.

[0272] In other embodiments, the apparatus may further include:

[0273] Flow rate acquisition module: used to acquire the current flow rate of the second liquid before adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information;

[0274] Flow rate determination module: used to determine the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid;

[0275] Accordingly, the speed control module 20 may specifically include:

[0276] The velocity ratio determination submodule is used to determine the current velocity ratio corresponding to the current interface position information and the target velocity ratio corresponding to the target interface position information based on the second preset correspondence. The second preset correspondence is used to characterize the correspondence between the preset velocity ratio and multiple interface position information of the laminar flow distribution interface in the converging flow channel.

[0277] Speed ​​regulation submodule: Used to adjust the flow rate of at least one of the first liquid and the second liquid based on the current flow rate of the second liquid and the current flow rate of the first liquid, until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information matches the target interface position information.

[0278] In some embodiments, the microfluidic device further includes a light sensor, the signal acquisition area of ​​which covers at least a portion of the confluence channel, for generating photosensitive information of the laminar flow in the confluence channel; the interface monitoring module 10 may include:

[0279] Photosensitive information acquisition submodule: used to acquire the photosensitive information collected by the photosensitive sensor;

[0280] Position analysis submodule: used to perform interface position analysis based on the photosensitive information to obtain the current interface position information.

[0281] In some embodiments, the light sensor is a camera, and the light-sensing information is a flow channel image acquired for the converging flow channel; the position analysis submodule may include:

[0282] Edge detection unit: used to perform edge detection on the flow channel image of the confluence flow channel walls and the laminar flow interface in the confluence flow channel, to obtain the first edge corresponding to the first sidewall, the second edge corresponding to the second sidewall and the third edge corresponding to the laminar flow interface, wherein the first sidewall and the second sidewall are two walls arranged opposite to each other on the confluence flow channel;

[0283] First position determination unit: used to determine the current interface position information based on the first distance between the first edge and the third edge, and the second distance between the second edge and the third edge.

[0284] In other embodiments, the light sensor is a camera, and the light-sensing information is a flow channel image acquired for the converging flow channel; the position analysis submodule may include:

[0285] Point coordinate acquisition unit: used to acquire the coordinate information of multiple feature points on the boundary line corresponding to the laminar flow interface based on the flow channel image;

[0286] Slope determination unit: used to determine the slope of the boundary line based on the coordinate information of multiple feature points;

[0287] The second position determination unit is used to determine the current interface position information based on the slope of the boundary line.

[0288] In some embodiments, the apparatus further includes a first flow rate ratio control module: used to control the flow rate of the first liquid in the first branch channel to stabilize, and to adjust the flow rate of the second liquid in the second branch channel to control the flow rate ratio; if the flow rate of the second liquid in the second branch channel reaches the upper limit or lower limit of the second liquid flow rate, to control the flow rate of the second liquid in the second branch channel to stabilize, and to adjust the flow rate of the first liquid in the first branch channel to control the flow rate ratio.

[0289] In some embodiments, the apparatus further includes a second flow rate ratio control module: used to control the flow rate of the second liquid in the second branch channel to stabilize, and to adjust the flow rate of the first liquid in the first branch channel to control the flow rate ratio; if the flow rate of the first liquid in the first branch channel reaches the upper limit or lower limit of the first liquid flow rate, to control the flow rate of the first liquid in the first branch channel to stabilize, and to adjust the flow rate of the second liquid in the second branch channel to control the flow rate ratio.

[0290] In some embodiments, the apparatus further includes a third flow rate ratio control module: used to control the flow rate of the second liquid in the second branch channel to stabilize and reduce the flow rate of the first liquid in the first branch channel if it is necessary to reduce the flow rate ratio between the first liquid and the second liquid.

[0291] Furthermore, the third flow rate ratio control module is also used to: if it is necessary to increase the flow rate ratio between the first liquid and the second liquid, control the flow rate of the first liquid in the first branch channel to stabilize, and decrease the flow rate of the second liquid in the second branch channel.

[0292] In some embodiments, at least one of the first branch channel and the second branch channel is connected to a flow regulating device. The speed regulating module 20 may be specifically used to: adjust the operating parameters of the flow regulating device to adjust the flow rate of the first liquid in the first branch channel connected to the flow regulating device or the flow rate of the second liquid in the second branch channel connected to the flow regulating device.

[0293] In some embodiments, the speed control module 20 may be specifically used to: adjust at least one of the flow rates of the first liquid and the second liquid under a first flow rate constraint, until the updated current interface position information matches the target interface position information; the first flow rate constraint is used to control the flow rate of the first liquid within a first flow rate range, the first flow rate range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel; and / or, to control the flow rate of the second liquid within a second flow rate range, the second flow rate range being the fluid velocity range required to maintain the laminar flow state of the second liquid in the second branch channel and the confluence channel.

[0294] In some embodiments, the first liquid is blood, and the speed control module 20 can be specifically used to: under the second flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information;

[0295] The second velocity constraint is used to control the velocity of the first liquid within the third velocity range. The third velocity range is the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel, and to prevent the first liquid from forming a coagulation state.

[0296] In some embodiments, the microfluidic device further includes a first detection device and a mixing channel communicating with the confluence channel. In the current operating mode, which is a mixed-liquid detection mode, the second liquid is a reaction solution capable of specifically reacting with the first liquid. The mixing channel is used to mix the target fluid so that the reaction solution mixes with the first liquid and undergoes a specific reaction to form the target mixed liquid. Accordingly, the device further includes:

[0297] The first detection module for the mixed liquid is used to detect the target mixed liquid based on the first detection device after adjusting at least one of the flow rates of the first liquid and the second liquid to match the updated current interface position information with the target interface position information to form the target fluid in the confluence channel, thereby obtaining first detection parameter data. The first detection parameter data is used to indicate the content information of the target substance in the target mixed liquid.

[0298] First Result Generation Module: Used to generate detection results based on the first detection parameter data.

[0299] In some embodiments, when the current working mode is the mixed liquid detection mode, the target interface position information is determined based on a preset mixing ratio between the first liquid and the second liquid.

[0300] In some embodiments, the first liquid is blood;

[0301] When the target substance includes an anticoagulant, the first detection parameter data includes detection data indicating the anticoagulant level of the blood.

[0302] In some embodiments, the apparatus further includes:

[0303] Interface position acquisition module: used to acquire the interface position information corresponding to the target mixture;

[0304] Mixing ratio determination module: used to determine the actual mixing ratio between the first liquid and the second liquid based on the interface position information corresponding to the target mixture;

[0305] Dilution ratio determination module: used to determine the first liquid dilution ratio corresponding to the target mixture based on the actual mixing ratio;

[0306] Accordingly, the first result generation module is specifically used to generate a test result based on the first detection parameter data and the first liquid dilution factor.

[0307] In some embodiments, the microfluidic device further includes a second detection device disposed on the confluence channel, and the second liquid is a reference solution; in the current working mode, which is the cleaning mode for the second detection device, the desired position corresponding to the target interface position information is that the laminar interface is deviated towards the first branch channel and the reference solution covers the detection area of ​​the second detection device.

[0308] In some embodiments, when the current working mode is a measurement mode based on the second detection device, the desired position corresponding to the target interface position information is the laminar flow interface deviating towards the second branch channel side and the first liquid covering the detection area of ​​the second detection device.

[0309] In some embodiments, in cleaning mode, the apparatus further includes:

[0310] The second detection module is used to adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form the target fluid in the confluence channel, and then acquire preset parameter data of the reference solution and second detection parameter data of the second detection device. The second detection parameter data is used to indicate the content information of the target marker in the reference solution flowing through the second detection device.

[0311] Cleaning status determination module: If the preset parameter data is consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

[0312] Furthermore, the device also includes:

[0313] First flow rate ratio adjustment module: used to adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid if the preset parameter data is inconsistent with the second detection parameter data and the second detection parameter data remains unchanged.

[0314] Data detection module: If the preset parameter data is found to be consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

[0315] Furthermore, the device also includes:

[0316] Location update module: Used to obtain updated interface location information when the preset parameter data and the updated second detection parameter data are consistent;

[0317] Position calibration module: used to calibrate the target interface position information corresponding to the current working mode based on the updated interface position information.

[0318] In some embodiments, in measurement mode, the apparatus may further include:

[0319] The third detection module is used to adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form the target fluid in the converging flow channel, and then acquire the third detection parameter data of the second detection device. The third detection parameter data is used to indicate the content information of the target marker in the first liquid flowing through the second detection device.

[0320] The second result generation module is used to generate a detection result based on the third detection parameter data if the third detection parameter data remains stable within a preset time period.

[0321] Furthermore, the device may also include:

[0322] Second flow rate ratio adjustment module: If the third detection parameter data is unstable within a preset time period, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid until the updated third detection parameter data remains stable within the preset time period;

[0323] The third result generation module is used to generate detection results based on updated third detection parameter data.

[0324] This application embodiment also provides a microfluidic system, including a microfluidic device and a fluid control device. The microfluidic device is provided with a first branch channel, a second branch channel and a converging channel, and the converging channel is connected to the first branch channel and the second branch channel respectively.

[0325] The fluid control device is used to monitor the current interface position information of the laminar interface in the confluence channel. The first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar interface is the interface between the first liquid and the second liquid in the confluence channel. If the current interface position information does not match the target interface position information corresponding to the current operating mode, at least one of the flow rates of the first liquid and the second liquid is adjusted until the updated current interface position information matches the target interface position information to form the target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar interface in the confluence channel under the current operating mode.

[0326] Optionally, the fluid control device includes the aforementioned flow regulation device. This fluid control device is used to execute the fluid control method mentioned in the above embodiments. The aforementioned microfluidic device and fluid control device can be connected to an extracorporeal blood circulation system, such as the mechanically assisted circulation system corresponding to an artificial heart or artificial lung (ECMO). The first branch channel can be connected to the extracorporeal blood circulation loop, and the corresponding first fluid can be blood. This enables real-time blood monitoring in the aforementioned microfluidic system, allowing for dynamic and continuous detection of blood parameters without the need for manual blood sampling, thus improving the convenience of blood monitoring.

[0327] It should be noted that the above-described device embodiments, system embodiments, and method embodiments are based on the same implementation methods.

[0328] This application provides a device, which can be a terminal or a server, including a processor and a memory. The memory stores at least one instruction or at least one program, which is loaded and executed by the processor to implement the microfluidic-based fluid control method or neural network training method provided in the above method embodiments.

[0329] Memory is used to store software programs and modules. The processor executes these stored software programs and modules to perform various functional applications and detect anomalies. Memory can primarily consist of a program storage area and a data storage area. The program storage area stores the operating system, application programs required for functionality, etc.; the data storage area stores data created based on device usage, etc. Furthermore, memory can include high-speed random access memory (RAM) and non-volatile memory, such as at least one disk storage device, flash memory device, or other volatile solid-state storage device. Accordingly, memory can also include a memory controller to provide the processor with access to the memory.

[0330] The methods and embodiments provided in this application can be executed in electronic devices such as mobile terminals, computer terminals, servers, or similar computing devices. Figure 12 This is a hardware structure block diagram of an electronic device based on a microfluidic fluid control method or neural network training method provided in an embodiment of this application. For example... Figure 12As shown, the electronic device 900 can vary significantly due to differences in configuration or performance. It may include one or more Central Processing Units (CPUs) 910 (CPUs 910 may include, but are not limited to, microprocessors such as MCUs or programmable logic devices such as FPGAs), a memory 930 for storing data, and one or more storage media 920 (e.g., one or more mass storage devices) for storing application programs 923 or data 922. The memory 930 and storage media 920 may be temporary or persistent storage. The program stored in the storage media 920 may include one or more modules, each module including a series of instruction operations on the electronic device. Furthermore, the CPU 910 may be configured to communicate with the storage media 920 and execute a series of instruction operations in the storage media 920 on the electronic device 900. The electronic device 900 may also include one or more power supplies 960, one or more wired or wireless network interfaces 950, one or more input / output interfaces 940, and / or one or more operating systems 921, such as Windows Server. TM Mac OS X TM Unix TM Linux™, FreeBSD™, etc.

[0331] The input / output interface 940 can be used to receive or send data via a network. Specific examples of the network described above may include a wireless network provided by the communication provider of the electronic device 900. In one example, the input / output interface 940 includes a network interface controller (NIC), which can connect to other network devices via a base station to communicate with the Internet. In another example, the input / output interface 940 may be a radio frequency (RF) module for wireless communication with the Internet.

[0332] Those skilled in the art will understand that Figure 12 The structure shown is for illustrative purposes only and does not limit the structure of the electronic device described above. For example, the electronic device 900 may also include... Figure 12 The more or fewer components shown, or having the same Figure 12 The different configurations shown.

[0333] Embodiments of this application also provide a computer-readable storage medium, which can be disposed in an electronic device to store at least one instruction or at least one program related to implementing an anomaly detection method in the method embodiments. The at least one instruction or the at least one program is loaded and executed by the processor to implement the anomaly detection method provided in the above method embodiments.

[0334] Optionally, in this embodiment, the storage medium may be located at at least one of the multiple network servers in a computer network. Optionally, in this embodiment, the storage medium may include, but is not limited to, various media capable of storing program code, such as USB flash drives, read-only memory (ROM), random access memory (RAM), portable hard drives, magnetic disks, or optical disks.

[0335] According to one aspect of this application, a computer program product or computer program is provided, comprising computer instructions stored in a computer-readable storage medium. A processor of an electronic device reads the computer instructions from the computer-readable storage medium and executes the computer instructions, causing the electronic device to perform the methods provided in the various alternative implementations described above.

[0336] The fluid control method, apparatus, device, storage medium, and microfluidic device based on microfluidics provided in this application can be applied to microfluidic devices. In these devices, a first liquid flowing from a first branch channel and a second liquid flowing from a second branch channel converge in a confluence channel to form laminar flow, creating a laminar interface between the first and second liquids. By monitoring the current interface position information in the confluence channel and comparing it with the target interface position information corresponding to the current operating mode, if a mismatch is found, the flow rates of the first and second liquids are adjusted. At least one of the flow rates is used to move the laminar interface toward the desired position corresponding to the target interface position information, until the updated current interface position information matches the target interface position information, thus obtaining the target laminar flow required for the current working mode; during the control process, the laminar interface position can be adjusted by adjusting the flow rates of the first liquid and / or the second liquid, thereby reaching the preset position, without the need to accurately determine and adjust the specific flow rate value of the solution, reducing the difficulty of control; furthermore, the technical solution of this application does not need to involve the relationship between different flow channels and flow rate data, is not affected by changes in the shape and size of the flow channel pipe, and has a wide range of applications.

[0337] It should be noted that the order of the embodiments described above is merely for descriptive purposes and does not represent the superiority or inferiority of the embodiments. Furthermore, the above description focuses on specific embodiments of this application. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps described in the claims can be performed in a different order than that shown in the embodiments and still achieve the desired results. Additionally, the processes depicted in the drawings do not necessarily require a specific or sequential order to achieve the desired results. In some implementations, multitasking and parallel processing are also possible or may be advantageous.

[0338] The various embodiments in this application are described in a progressive manner. Similar or identical parts between embodiments can be referred to mutually. Each embodiment focuses on describing the differences from other embodiments. In particular, the device, equipment, and storage medium embodiments are basically similar to the method embodiments, so the descriptions are relatively simple; relevant parts can be referred to the descriptions of the method embodiments.

[0339] Those skilled in the art will understand that all or part of the steps of the above embodiments can be implemented by hardware, or by a program instructing the relevant hardware to implement them. The program can be stored in a computer-readable storage medium, such as a read-only memory, a disk, or an optical disk.

[0340] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A microfluidic-based fluid control method, applied to a microfluidic device, wherein the microfluidic device is provided with a first branch channel, a second branch channel, and a converging channel, the converging channel being connected to the first branch channel and the second branch channel respectively, characterized in that, The method includes: The current interface position information of the laminar flow interface in the confluence channel is monitored. The first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar flow interface is the interface between the first liquid and the second liquid in the confluence channel. If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rate of the first liquid and the flow rate of the second liquid until the updated current interface position information matches the target interface position information, so as to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar interface in the confluence channel under the current working mode. The target interface location information is determined in the following way: The target interface location information corresponding to the current working mode is determined based on the first preset correspondence relationship, which is used to characterize the correspondence between multiple working modes of the microfluidic device and the preset location information.

2. The method according to claim 1, characterized in that, Adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes: Based on the target interface position information and the current interface position information, determine the offset direction of the laminar flow interface relative to the desired position; If the offset direction is the laminar flow interface shifting towards the first branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid, until the laminar flow interface moves towards the second branch channel side until the updated current interface position information matches the target interface position information.

3. The method according to claim 2, characterized in that, The method further includes: If the offset direction is the laminar flow interface shifting towards the second branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid, until the laminar flow interface moves towards the first branch channel side until the updated current interface position information matches the target interface position information.

4. The method according to claim 1, characterized in that, Before adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, the method further includes: Obtain the current flow rate of the second liquid; determine the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid.

5. The method according to claim 4, characterized in that, Adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes: Based on the second preset correspondence, the current flow rate ratio corresponding to the current interface position information and the target flow rate ratio corresponding to the target interface position information are determined. The second preset correspondence is used to characterize the correspondence between the preset flow rate ratio and the multiple interface position information of the laminar flow interface in the converging flow channel. Based on the current flow rate of the second liquid and the current flow rate of the first liquid, the flow rate of at least one of the first liquid and the second liquid is adjusted until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information matches the target interface position information.

6. The method according to claim 1, characterized in that, The microfluidic device further includes a photosensitive sensor, the signal acquisition area of ​​which covers at least a portion of the confluence channel, for generating photosensitive information of the laminar flow in the confluence channel; the current interface position information is determined in the following manner: Acquire the photosensitized information collected by the optical sensor; Based on the photosensitive information, the interface position is analyzed to obtain the current interface position information.

7. The method according to claim 6, characterized in that, The light sensor is a camera, and the photosensitive information is a flow channel image captured for the converging flow channel; the step of analyzing the interface position based on the photosensitive information to obtain the current interface position information includes: Edge detection is performed on the flow channel image of the confluence flow channel and the laminar flow interface in the confluence flow channel to obtain the first edge corresponding to the first sidewall, the second edge corresponding to the second sidewall and the third edge corresponding to the laminar flow interface. The first sidewall and the second sidewall are two walls that are arranged opposite to each other on the confluence flow channel. The current interface position information is determined based on the first distance between the first edge and the third edge, and the second distance between the second edge and the third edge.

8. The method according to claim 6, characterized in that, The light sensor is a camera, and the photosensitive information is a flow channel image captured for the converging flow channel; the step of analyzing the interface position based on the photosensitive information to obtain the current interface position information includes: Based on the flow channel image, obtain the coordinate information of multiple feature points on the boundary line corresponding to the laminar flow interface; Based on the coordinate information of the multiple feature points, the slope of the dividing line is determined; The current interface position information is determined based on the slope of the dividing line.

9. The method according to claim 2, characterized in that, The flow rate ratio is controlled in the following manner: The flow rate of the first liquid in the first branch channel is controlled to be stable, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio. If the flow rate of the second liquid in the second branch channel reaches the upper limit or lower limit of the second liquid flow rate, the flow rate of the second liquid in the second branch channel is stabilized, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio.

10. The method according to claim 2, characterized in that, The flow rate ratio is controlled in the following manner: The flow rate of the second liquid in the second branch channel is controlled to be stable, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio. If the flow rate of the first liquid in the first branch channel reaches the upper limit or lower limit of the first liquid flow rate, the flow rate of the first liquid in the first branch channel is stabilized, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio.

11. The method according to claim 2, characterized in that, The flow rate ratio is controlled in the following manner: If it is necessary to reduce the flow rate ratio between the first liquid and the second liquid, and control the flow rate of the second liquid in the second branch channel to stabilize, then reduce the flow rate of the first liquid in the first branch channel.

12. The method according to claim 2, characterized in that, The flow rate ratio is controlled in the following manner: If it is necessary to increase the flow rate ratio between the first liquid and the second liquid, control the flow rate of the first liquid in the first branch channel to stabilize, and decrease the flow rate of the second liquid in the second branch channel.

13. The method according to claim 1, characterized in that, At least one of the first branch flow channel and the second branch flow channel is connected to a flow regulating device, and the flow rate of the first liquid or the flow rate of the second liquid is regulated in the following manner: Adjust the operating parameters of the flow regulating device to regulate the flow rate of the first liquid in the first branch channel connected to the flow regulating device or the flow rate of the second liquid in the second branch channel connected to the flow regulating device.

14. The method according to any one of claims 1-13, characterized in that, Adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes: Under the first flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information; The first flow velocity constraint condition is used to control the flow velocity of the first liquid within a first flow velocity range, the first flow velocity range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel; and / or, the first flow velocity constraint condition is used to control the flow velocity of the second liquid within a second flow velocity range, the second flow velocity range being the fluid velocity range required to maintain the laminar flow state of the second liquid in the second branch channel and the confluence channel.

15. The method according to any one of claims 1-13, characterized in that, The first liquid is blood, and adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information includes: Under the second flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information; The second flow velocity constraint is used to control the flow velocity of the first liquid within a third flow velocity range, which is the fluid velocity range required to maintain the first liquid in a laminar flow state in the first branch channel and the confluence channel, and to prevent the first liquid from forming a coagulation state.

16. The method according to any one of claims 1-13, characterized in that, The microfluidic device further includes a first detection device and a mixing channel connected to the confluence channel. In the current working mode, which is the mixed liquid detection mode, the second liquid is a reaction solution that can specifically react with the first liquid. The mixing channel is used to mix the target fluid so that the reaction solution mixes with the first liquid and undergoes a specific reaction to form a target mixed liquid. After adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel, the method further includes: The target mixture is tested using the first detection device to obtain first detection parameter data, which is used to indicate the content information of the target substance in the target mixture. The detection result is generated based on the first detection parameter data.

17. The method according to claim 16, characterized in that, In the current working mode of mixed liquid detection, the target interface position information is determined based on a preset mixing ratio between the first liquid and the second liquid.

18. The method according to claim 16, characterized in that, The first liquid is blood; When the target substance includes an anticoagulant, the first detection parameter data includes detection data indicating the anticoagulant level of the blood.

19. The method according to claim 16, characterized in that, The method further includes: Obtain the interface position information corresponding to the target mixture; Based on the interface position information corresponding to the target mixture, the actual mixing ratio between the first liquid and the second liquid is determined. The first liquid dilution factor corresponding to the target mixture is determined based on the actual mixing ratio. The step of generating the detection result based on the first detection parameter data includes: The detection result is generated based on the first detection parameter data and the first liquid dilution factor.

20. The method according to any one of claims 1-13, characterized in that, The microfluidic device further includes a second detection device disposed on the confluence channel, wherein the second liquid is a reference solution; In the current working mode, which is the cleaning mode for the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface is deviated from the first branch channel side and the reference solution covers the detection area of ​​the second detection device.

21. The method according to any one of claims 1-13, characterized in that, The microfluidic device also includes a second detection device disposed on the confluence channel; In the current working mode, which is the measurement mode based on the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface deviates towards the second branch channel and the first liquid covers the detection area of ​​the second detection device.

22. The method according to claim 20, characterized in that, In the cleaning mode, after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form the target fluid in the confluence channel, the method further includes: Acquire preset parameter data of the reference solution and second detection parameter data of the second detection device, wherein the second detection parameter data is used to indicate the content information of the target marker in the reference solution flowing through the second detection device; If the preset parameter data is consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

23. The method according to claim 22, characterized in that, The method further includes: If the preset parameter data is inconsistent with the second detection parameter data, and the second detection parameter data remains stable, adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid. If the preset parameter data is found to be consistent with the second detection parameter data, it is determined that the cleaning of the second detection device is complete.

24. The method according to claim 23, characterized in that, The method further includes: When the preset parameter data and the updated second detection parameter data are consistent, the updated interface position information is obtained; Based on the updated interface position information, calibrate the target interface position information corresponding to the current working mode.

25. The method according to claim 20, characterized in that, In measurement mode, after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel, the method further includes: Acquire third detection parameter data of the second detection device, wherein the third detection parameter data is used to indicate the content information of the target marker in the first liquid flowing through the second detection device; If the third detection parameter data remains stable within a preset time period, a detection result is generated based on the third detection parameter data.

26. The method according to claim 25, characterized in that, The method further includes: If the third detection parameter data is unstable within a preset time period, at least one of the flow rates of the first liquid and the second liquid is adjusted to increase the flow rate ratio between the first liquid and the second liquid until the updated third detection parameter data remains stable within the preset time period. The detection results are generated based on the updated third detection parameter data.

27. A microfluidic-based fluid control device, characterized in that, An application in microfluidic devices, the microfluidic device comprising a first branch channel, a second branch channel, and a converging channel, the converging channel being connected to the first branch channel and the second branch channel respectively, characterized in that the device comprises: Interface monitoring module: used to monitor the current interface position information of the laminar flow interface in the confluence channel; the first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow, and the laminar flow interface is the interface between the first liquid and the second liquid in the confluence channel; Speed ​​control module: If the current interface position information does not match the target interface position information corresponding to the current working mode, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information, so as to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar flow interface in the confluence channel under the current working mode. The target interface location information is determined in the following way: The target interface location information corresponding to the current working mode is determined based on the first preset correspondence relationship, which is used to characterize the correspondence between multiple working modes of the microfluidic device and the preset location information.

28. The apparatus according to claim 27, characterized in that, The speed control module includes: Offset Direction Determination Submodule: Used to determine the offset direction of the laminar flow interface relative to the desired position based on the target interface position information and the current interface position information; First adjustment submodule: If the offset direction is the laminar flow interface shifting towards the first branch channel side, adjust at least one of the flow rates of the first liquid and the second liquid to increase the flow rate ratio between the first liquid and the second liquid, so that the laminar flow interface moves towards the second branch channel side until the updated current interface position information matches the target interface position information.

29. The apparatus according to claim 28, characterized in that, The device further includes: The second adjustment submodule is used to adjust at least one of the flow rates of the first liquid and the second liquid if the offset direction is the laminar flow interface shifting towards the second branch channel side, so as to reduce the flow rate ratio between the first liquid and the second liquid, until the laminar flow interface moves towards the first branch channel side until the updated current interface position information matches the target interface position information.

30. The apparatus according to claim 27, characterized in that, The device further includes: Flow rate acquisition module: used to acquire the current flow rate of the second liquid before adjusting at least one of the flow rates of the first liquid and the second liquid, until the updated current interface position information matches the target interface position information; Flow rate determination module: used to determine the current flow rate of the first liquid based on the current flow rate ratio and the current flow rate of the second liquid.

31. The apparatus according to claim 30, characterized in that, The speed control module includes: Flow rate ratio determination submodule: used to determine the current flow rate ratio corresponding to the current interface position information and the target flow rate ratio corresponding to the target interface position information based on the second preset correspondence relationship. The second preset correspondence relationship is used to characterize the correspondence relationship between the preset flow rate ratio and multiple interface position information of the laminar flow interface in the converging flow channel. Speed ​​regulation submodule: used to adjust the flow rate of at least one of the first liquid and the second liquid based on the current flow rate of the second liquid and the current flow rate of the first liquid, until the flow rate ratio of the second liquid to the first liquid reaches the target flow rate ratio, so that the updated current interface position information matches the target interface position information.

32. The apparatus according to claim 27, characterized in that, The microfluidic device further includes an optical sensor, the signal acquisition area of ​​which covers at least a portion of the confluence channel, for generating photosensitive information of the laminar flow in the confluence channel; The interface monitoring module includes: Photosensitive information acquisition submodule: used to acquire the photosensitive information collected by the photosensitive sensor; Position analysis submodule: used to perform interface position analysis based on the photosensitive information to obtain the current interface position information.

33. The apparatus according to claim 32, characterized in that, The light sensor is a camera, and the light-sensing information is a flow channel image acquired for the converging flow channel; the position analysis submodule includes: Edge detection unit: used to perform edge detection on the flow channel image of the confluence flow channel and the laminar flow interface in the confluence flow channel, to obtain the first edge corresponding to the first sidewall, the second edge corresponding to the second sidewall and the third edge corresponding to the laminar flow interface, wherein the first sidewall and the second sidewall are two walls arranged opposite to each other on the confluence flow channel; First position determination unit: used to determine the current interface position information based on a first distance between the first edge and the third edge, and a second distance between the second edge and the third edge.

34. The apparatus according to claim 32, characterized in that, The light sensor is a camera, and the light-sensing information is a flow channel image acquired for the converging flow channel; the position analysis submodule includes: Point coordinate acquisition unit: used to acquire coordinate information of multiple feature points on the boundary line corresponding to the laminar flow interface based on the flow channel image; Slope determination unit: used to determine the slope of the boundary line based on the coordinate information of the multiple feature points; Second position determination unit: used to determine the current interface position information based on the slope of the dividing line.

35. The apparatus according to claim 28, characterized in that, The device further includes a first flow rate ratio control module, used for: The flow rate of the first liquid in the first branch channel is controlled to be stable, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio. If the flow rate of the second liquid in the second branch channel reaches the upper limit or lower limit of the second liquid flow rate, the flow rate of the second liquid in the second branch channel is stabilized, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio.

36. The apparatus according to claim 28, characterized in that, The device further includes a second flow rate ratio control module, used for: The flow rate of the second liquid in the second branch channel is controlled to be stable, and the flow rate of the first liquid in the first branch channel is adjusted to regulate the flow rate ratio. If the flow rate of the first liquid in the first branch channel reaches the upper limit or lower limit of the first liquid flow rate, the flow rate of the first liquid in the first branch channel is stabilized, and the flow rate of the second liquid in the second branch channel is adjusted to regulate the flow rate ratio.

37. The apparatus according to claim 28, characterized in that, The device further includes a third flow rate ratio control module, used for: If it is necessary to reduce the flow rate ratio between the first liquid and the second liquid, and control the flow rate of the second liquid in the second branch channel to stabilize, then reduce the flow rate of the first liquid in the first branch channel.

38. The apparatus according to claim 28, characterized in that, The third flow rate ratio control module is also used to: if it is necessary to increase the flow rate ratio between the first liquid and the second liquid, control the flow rate of the first liquid in the first branch channel to stabilize, and decrease the flow rate of the second liquid in the second branch channel.

39. The apparatus according to claim 27, characterized in that, At least one of the first branch flow channel and the second branch flow channel is connected to a flow regulating device, and the speed regulating module is specifically used for: Adjust the operating parameters of the flow regulating device to regulate the flow rate of the first liquid in the first branch channel connected to the flow regulating device or the flow rate of the second liquid in the second branch channel connected to the flow regulating device.

40. The apparatus according to any one of claims 27-39, characterized in that, The speed control module is specifically used for: Under the first flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information; The first flow velocity constraint condition is used to control the flow velocity of the first liquid within a first flow velocity range, the first flow velocity range being the fluid velocity range required to maintain the laminar flow state of the first liquid in the first branch channel and the confluence channel; and / or, the first flow velocity constraint condition is used to control the flow velocity of the second liquid within a second flow velocity range, the second flow velocity range being the fluid velocity range required to maintain the laminar flow state of the second liquid in the second branch channel and the confluence channel.

41. The apparatus according to any one of claims 27-39, characterized in that, The first liquid is blood, and the speed control module is specifically used for: Under the second flow rate constraint, adjust at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information; The second flow velocity constraint is used to control the flow velocity of the first liquid within a third flow velocity range, which is the fluid velocity range required to maintain the first liquid in a laminar flow state in the first branch channel and the confluence channel, and to prevent the first liquid from forming a coagulation state.

42. The apparatus according to any one of claims 27-39, characterized in that, The microfluidic device further includes a first detection device and a mixing channel connected to the confluence channel. In the current working mode, which is the mixed liquid detection mode, the second liquid is a reaction solution that can specifically react with the first liquid. The mixing channel is used to mix the target fluid so that the reaction solution mixes with the first liquid and undergoes a specific reaction to form a target mixed liquid. The device further includes: The first detection module for the mixed liquid is used to detect the target mixed liquid based on the first detection device after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel, thereby obtaining first detection parameter data. The first detection parameter data is used to indicate the content information of the target substance in the target mixed liquid. First result generation module: used to generate detection results based on the first detection parameter data.

43. The apparatus according to claim 42, characterized in that, In the current working mode of mixed liquid detection, the target interface position information is determined based on a preset mixing ratio between the first liquid and the second liquid.

44. The apparatus according to claim 42, characterized in that, The first liquid is blood; When the target substance includes an anticoagulant, the first detection parameter data includes detection data indicating the anticoagulant level of the blood.

45. The apparatus according to claim 44, characterized in that, The device further includes: Interface position acquisition module: used to acquire the interface position information corresponding to the target mixture; Mixing ratio determination module: used to determine the actual mixing ratio between the first liquid and the second liquid based on the interface position information corresponding to the target mixture; Dilution ratio determination module: used to determine the first liquid dilution ratio corresponding to the target mixture based on the actual mixing ratio; The first result generation module is specifically used for: The detection result is generated based on the first detection parameter data and the first liquid dilution factor.

46. ​​The apparatus according to any one of claims 27-39, characterized in that, The microfluidic device further includes a second detection device disposed on the confluence channel, wherein the second liquid is a reference solution; In the current working mode, which is the cleaning mode for the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface is deviated from the first branch channel side and the reference solution covers the detection area of ​​the second detection device.

47. The apparatus according to any one of claims 27-39, characterized in that, The microfluidic device also includes a second detection device disposed on the confluence channel; In the current working mode, which is the measurement mode based on the second detection device, the desired position corresponding to the target interface position information is when the laminar flow interface deviates towards the second branch channel and the first liquid covers the detection area of ​​the second detection device.

48. The apparatus according to claim 46, characterized in that, In the cleaning mode, the device further includes: The second detection module is used to acquire preset parameter data of the reference solution and second detection parameter data of the second detection device after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel. The second detection parameter data is used to indicate the content information of the target marker in the reference solution flowing through the second detection device. Cleaning status determination module: used to determine that the cleaning of the second detection device is complete if the preset parameter data is consistent with the second detection parameter data.

49. The apparatus according to claim 48, characterized in that, The device further includes: First flow rate ratio adjustment module: used to adjust at least one of the flow rates of the first liquid and the second liquid to reduce the flow rate ratio between the first liquid and the second liquid if the preset parameter data is inconsistent with the second detection parameter data and the second detection parameter data remains stable; Data detection module: used to determine that the cleaning of the second detection device is complete if the preset parameter data is detected to be consistent with the second detection parameter data.

50. The apparatus according to claim 49, characterized in that, The device further includes: Location update module: used to obtain updated interface location information when the preset parameter data and the updated second detection parameter data are consistent; Position calibration module: used to calibrate the target interface position information corresponding to the current working mode based on the updated interface position information.

51. The apparatus according to claim 46, characterized in that, In measurement mode, the device further includes: The third detection module is used to acquire third detection parameter data of the second detection device after adjusting at least one of the flow rates of the first liquid and the second liquid until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel. The third detection parameter data is used to indicate the content information of the target marker in the first liquid flowing through the second detection device. The second result generation module is used to generate a detection result based on the third detection parameter data if the third detection parameter data remains stable within a preset time period.

52. The apparatus according to claim 51, characterized in that, The device further includes: The second flow rate ratio adjustment module is used to adjust at least one of the flow rates of the first liquid and the second liquid if the third detection parameter data is unstable within a preset time period, so as to increase the flow rate ratio between the first liquid and the second liquid until the updated third detection parameter data remains stable within the preset time period. The third result generation module is used to generate detection results based on the updated third detection parameter data.

53. A microfluidic system, characterized in that, It includes a microfluidic device and a fluid control device. The microfluidic device is provided with a first branch channel, a second branch channel and a converging channel. The converging channel is connected to the first branch channel and the second branch channel respectively. The fluid control device is used to monitor the current interface position information of the laminar interface in the confluence channel. The first liquid flowing out of the first branch channel and the second liquid flowing out of the second branch channel merge in the confluence channel to form laminar flow. The laminar interface is the interface between the first liquid and the second liquid in the confluence channel. If the current interface position information does not match the target interface position information corresponding to the current operating mode, at least one of the flow rates of the first liquid and the second liquid is adjusted until the updated current interface position information matches the target interface position information to form a target fluid in the confluence channel. The target interface position information is used to indicate the desired position of the laminar interface in the confluence channel under the current operating mode.

54. A computer-readable storage medium, characterized in that, The storage medium stores at least one instruction or at least one program segment, which is loaded and executed by a processor to implement the microfluidic-based fluid control method as described in any one of claims 1-26.

55. An electronic device, characterized in that, The device includes a processor and a memory, the memory storing at least one instruction or at least one program, the at least one instruction or the at least one program being loaded and executed by the processor to implement the microfluidic-based fluid control method as described in any one of claims 1-26.

Images