Wrinkled papaya biological organic and inorganic fertilizer and preparation method thereof

Abstract

The invention belongs to the technical field of fertilizer manufacturing, and specifically relates to a wrinkled papaya bio-organic-inorganic fertilizer and a preparation method thereof. The bio-organic-inorganic fertilizer comprises, in percentage, 48.48% to 71.28% of bio-organic fertilizer, 28.58% to 51.38% of inorganic fertilizer, 0.1% of a deep-ploughing-free soil conditioner, 0.03% of Bacillus subtilis A, and 0.01% of Azospirillum. The preparation method of the wrinkled papaya bio-organic-inorganic fertilizer is as follows: step 1, preparing organic fertilizer A; step 2, preparing organic fertilizer B; and step 3, preparing the bio-organic-inorganic fertilizer. The bio-organic-inorganic fertilizer specifically for the wrinkled papaya loosens soil and breaks soil compaction by using the deep-ploughing-free soil conditioner, thereby improving fertilizer utilization rate and reducing fertilizer usage. Meanwhile, loosening the soil can promote the wrinkled papaya to absorb nutrient elements, thereby enhancing the resilience of the wrinkled papaya.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer manufacturing, in particular to a wrinkled papaya biological organic-inorganic fertilizer and a preparation method thereof. Background Art

[0002] my country is a major livestock and poultry breeding country and a major agricultural production country. According to statistics, the annual amount of livestock and poultry manure produced is about 17.3×10 8 Tons. However, livestock and poultry breeding pollution has a large amount of emissions and high concentrations, which will cause serious pollution to the water ecological environment, making agricultural planting areas face serious problems such as waste disposal and environmental pollution.

[0003] Tiquia et al. studied the composting of livestock and poultry manure and garden waste; Le et al. studied the effects of inoculation agents and pH value on the structure of microbial communities during composting; Chen Guangyin et al. published "Study on nutrient changes during the combined composting of mushroom residue and fallen leaves" in the Journal of Agricultural Environmental Science, and Li Fan et al. published "The effect of chicken manure and mushroom residue composting technology on the composting effect"; He Sufen studied "Methods of preparing bio-organic fertilizer using mushroom residue"; At present, there are no reports or products on the production of organic and inorganic bio-fertilizers specifically for wrinkled papaya using cassava residue, poultry manure and microbial agents for rapid composting.

[0004] Wrinkled papaya is primarily found in Linyi City, Shandong Province. Containing 17 amino acids and a variety of nutrients, regular consumption can soothe the liver and stomach, relax tendons and activate joints, soften muscles, and fight cancer. It also has hangover-relieving properties. Combining ornamental, edible, and medicinal uses, it represents a unique fruit tree germplasm resource found in northern my country. Tanghe Town, renowned as the "Hometown of Papaya in Yizhou," boasted 16,000 mu (approximately 16,000 mu) of cultivated area in Tanghe Town alone in 2022, with 8 million potted plants. Linyi currently boasts four series and over 20 varieties of wrinkled papaya, characterized by strong adaptability, rapid growth, easy flowering, ease of management, and high profitability.

[0005] Zhao Jiancheng, Liu Honglin, Yan Xiaopeng, Cao Banghua and others published a study on the changes in the main components of wrinkled papaya during fruit development in Shaanxi Agricultural Science; Liu Guili and Xu Tongyin published the cultivation technology of wrinkled papaya in Shizhen Traditional Chinese Medicine; Wang Yuebing and Huo Changliang published the high-yield cultivation technology of wrinkled papaya in Hebei Fruit Trees; Wang Jiaxiang published the functional component analysis and main processing product research and development of Yizhou papaya, evaluation and utilization of the ornamental characteristics of Shandong Yizhou papaya resources in Food Science; there are no research reports on biological, organic and inorganic fertilizers related to wrinkled papaya.

[0006] Therefore, we propose a special bio-organic-inorganic fertilizer for wrinkled papaya produced using chicken manure, cassava residue and chemical fertilizers, which is used to promote the absorption of nutrients by wrinkled papaya to increase yield and improve quality, and to reuse livestock and poultry manure to reduce environmental pollution. Summary of the Invention

[0007] The object of the present invention is to provide a kind of wrinkled papaya biological organic-inorganic fertilizer and preparation method thereof, in order to solve the problem that the existing wrinkled papaya proposed in the above-mentioned background technology lacks special biological organic-inorganic fertilizer.

[0008] To achieve the above object, the present invention provides the following technical solution: a wrinkled papaya biological organic and inorganic fertilizer, the biological organic and inorganic fertilizer comprising in percentage;

[0009] Bio-organic fertilizer 48.48% to 71.28%, the bio-organic fertilizer includes organic fertilizer A by mass

[0010] 30.28-66.28 parts, organic fertilizer B 5-18.2 parts;

[0011] 28.58% to 51.38% of inorganic fertilizer; the inorganic fertilizer includes, by mass, 124 to 196 parts of urea, 25 to 91 parts of monoammonium phosphate, 130 to 220 parts of potassium sulfate, and 6.8 to 8.6 parts of zinc sulfate;

[0012] No-deep-tillage soil conditioner 0.1%;

[0013] Bacillus subtilis A 0.03%;

[0014] Azospirillum 0.01%;

[0015] The organic fertilizer A comprises chicken manure, cassava residue, and a composite bacterial agent of Thermophilic Sporotrichum spp. YM-2, and the organic fertilizer A is prepared by fermenting 70 parts of chicken manure and 30 parts of cassava residue in the presence of 0.1 parts of the composite bacterial agent of Thermophilic Sporotrichum spp. YM-2 in parts by mass;

[0016] The organic fertilizer B comprises wrinkled papaya waste, a composite bacterial agent of thermophilic Sporotrichum syringae YM-2 and Bacillus subtilis B. The organic fertilizer B is prepared by fermenting 100 parts of wrinkled papaya waste in the presence of 0.1 parts of the composite bacterial agent of thermophilic Sporotrichum syringae YM-2 and 0.01 parts of Bacillus subtilis B, based on mass.

[0017] Preferably, the Bacillus subtilis A is Bacillus subtilis HG-15, and the density of Bacillus subtilis A in the bio-organic-inorganic fertilizer is greater than or equal to 40 million / gram; and the Bacillus subtilis B is Bacillus subtilis HD11.

[0018] Preferably, the bio-organic-inorganic fertilizer contains 71.28% bio-organic fertilizer;

[0019] The bio-organic fertilizer comprises, by mass, 66.28 parts of organic fertilizer A and 5 parts of organic fertilizer B;

[0020] The inorganic fertilizer includes 124 parts of urea, 25 parts of monoammonium phosphate, 130 parts of potassium sulfate, and 6.8 parts of zinc sulfate in parts by mass.

[0021] Preferably, the bio-organic-inorganic fertilizer contains 62.6% bio-organic fertilizer;

[0022] The bio-organic fertilizer comprises, by mass, 49 parts of organic fertilizer A and 13.6 parts of organic fertilizer B;

[0023] The inorganic fertilizer includes 136 parts of urea, 68 parts of monoammonium phosphate, 160 parts of potassium sulfate, and 8.6 parts of zinc sulfate in parts by mass.

[0024] Preferably, the bio-organic-inorganic fertilizer contains 48.48% bio-organic fertilizer;

[0025] The bio-organic fertilizer comprises, by mass, 30.28 parts of organic fertilizer A and 18.2 parts of organic fertilizer B;

[0026] The inorganic fertilizer includes 196 parts of urea, 91 parts of monoammonium phosphate, 220 parts of potassium sulfate, and 6.8 parts of zinc sulfate in parts by mass.

[0027] A preparation method of wrinkled papaya biological organic and inorganic fertilizer, the preparation method of the wrinkled papaya biological organic and inorganic fertilizer is as follows:

[0028] Step 1: Preparation of organic fertilizer A:

[0029] A1. Transfer chicken manure and cassava residue in a ratio of 7:3 to a livestock and poultry manure harmless treatment device. Heat the device to 65°C to sterilize the chicken manure and cassava residue.

[0030] A2. Lower the heating temperature of the livestock and poultry manure harmless treatment equipment to 50°C, then evenly spray the composite bacterial agent of Thermophilic Sporotrichum YM-2 onto the chicken manure and cassava residue, and then ferment and compost the chicken manure and cassava residue;

[0031] A3. After the chicken manure and cassava residue have reached 60% maturity, remove them and compost them until they are fully mature, thereby obtaining organic fertilizer A.

[0032] Step 2: Preparation of organic fertilizer B:

[0033] B1, transporting the wrinkled papaya waste to a fermentation tank and spraying the composite bacterial agent of Thermospora Sporotrichum YM-2 for fermentation;

[0034] B2, after the wrinkled papaya waste degree of maturity reached 60%, sprayed bacillus subtilis B to the wrinkled papaya waste and continued to ferment, to the wrinkled papaya waste fully mature, can obtain organic fertilizer B;

[0035] Step 3: Preparation of biological organic and inorganic fertilizers:

[0036] C1. Grinding urea, monoammonium phosphate, potassium sulfate, and zinc sulfate respectively to obtain urea, monoammonium phosphate, potassium sulfate, and zinc sulfate powders;

[0037] C2. Fully mix urea powder, zinc sulfate powder, and a soil conditioner that does not require deep tillage to obtain a mixture A; fully mix monoammonium phosphate powder and organic fertilizer B to obtain a mixture B; and fully mix potassium sulfate powder and organic fertilizer A to obtain a mixture C.

[0038] C3. First, fully mix mixture A, mixture C and Azospirillum, and then add mixture B and Bacillus subtilis A and mix them thoroughly to obtain a semi-finished bio-organic fertilizer;

[0039] C4. Granulate and pack the semi-finished bio-organic and inorganic fertilizers to produce bio-organic and inorganic fertilizers.

[0040] Preferably, in the step 1, before mixing the chicken manure and cassava residue, the moisture content of the chicken manure and cassava residue is first detected to ensure that the moisture content of the chicken manure and cassava residue is 55% to 65%;

[0041] When the water content is higher than 65%, the chicken manure and cassava residue are dehydrated and the sewage is retained; when the water content is lower than 55%, the separated sewage or tap water is used to replenish the chicken manure and cassava residue.

[0042] Preferably, in the steps 1 and 2, when spraying the composite bacterial agent of Thermospora Sporotrichum YM-2, the chicken manure, cassava residue and wrinkled papaya waste are turned over.

[0043] Preferably, when spraying Bacillus subtilis B in step 2, the temperature of the fermentation tank is first lowered to room temperature, and then Bacillus subtilis B is sprayed. After spraying Bacillus subtilis B, the temperature of the fermentation tank is raised to 40°C.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] 1) The present invention obtains organic fertilizer A and organic fertilizer B by fermenting chicken manure, cassava residue, and wrinkled papaya waste; then, the organic colloid in the organic fertilizer A absorbs potassium ions in potassium sulfate, so that the potassium ions can be slowly released during use of the biological organic-inorganic fertilizer, thereby reducing the loss of the fertilizer and improving the use effect of the fertilizer; the gamma-polyglutamic acid in the organic fertilizer B protects phosphorus, thereby preventing phosphorus from crystallizing when encountering calcium ions in chicken manure and calcium ions in the soil, and free phosphorus is more convenient to be absorbed by the wrinkled papaya plant; and nitrogen fixing is also carried out by the genus Azospirillum, thereby increasing the nitrogen content of the soil, thereby ensuring that the wrinkled papaya plant and the fruit can have a good growth momentum;

[0046] 2) The special biological organic-inorganic fertilizer for wrinkled papaya of the present invention can loosen the soil by a soil conditioner without deep tillage to break soil compaction, thereby improving fertilizer utilization rate and thus reducing fertilizer usage; loosening the soil can also promote the absorption of nutrients by wrinkled papaya, thereby enhancing the resilience of wrinkled papaya; and by improving the utilization rate of fertilizer, can also increase the content of amino acids and vitamin C in wrinkled papaya fruits, thereby not only improving the nutrients of wrinkled papaya fruits, but also improving the quality and appearance coloring of wrinkled papaya fruits;

[0047] 3) The special biological organic and inorganic fertilizer for wrinkled papaya can not only solve the problem of poultry farming manure, but also turn agricultural waste from nearby breeding and planting into treasure, thereby reducing environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 This is a schematic diagram of the production process. DETAILED DESCRIPTION

[0049] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0050] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0051] Example 1:

[0052] See also Figure 1 The present invention provides a technical solution: a biological organic and inorganic fertilizer for wrinkled papaya, which is applied as a base fertilizer when the wrinkled papaya seedlings are first planted;

[0053] The bio-organic-inorganic fertilizer comprises, by percentage, 71.28% of bio-organic fertilizer, 28.58% of inorganic fertilizer, 0.1% of soil conditioner without deep tillage, 0.03% of Bacillus subtilis A and 0.01% of Azospirillum; the bio-organic fertilizer comprises, by mass, 66.28 parts of organic fertilizer A and 5 parts of organic fertilizer B; the organic fertilizer A is made by fermenting chicken manure and cassava residue in a ratio of 7:3 under the condition of a composite bacterial agent of Thermospora spp. YM-2; the organic fertilizer B is made by fermenting 100 parts of wrinkled papaya waste. The invention relates to a bio-organic inorganic fertilizer prepared by fermenting a compound bacterial agent of Thermospora Sporotrichum YM-2 and Bacillus subtilis B; the inorganic fertilizer comprises, by mass, 124 parts of urea, 25 parts of monoammonium phosphate, 130 parts of potassium sulfate, and 6.8 parts of zinc sulfate; the ratio of N, P2O5, and K2O in the prepared bio-organic inorganic fertilizer is 6:2.5:6, the total mass fraction of N, P2O5, and K2O is ≥15%, the zinc content is ≥0.2%, the mass fraction of organic matter is ≥32%, and the density of Bacillus subtilis is ≥40 million / g.

[0054] To produce one ton of wrinkled papaya bio-organic-inorganic fertilizer, 285.8 kg of inorganic fertilizer, 712.8 kg of bio-organic fertilizer, 1 kg of deep-tillage-free soil conditioner, 0.3 kg of Bacillus subtilis A, and 0.1 kg of Azospirillum are needed; among them, 124 kg of urea, 25 kg of monoammonium phosphate, 130 kg of potassium sulfate, and 6.8 kg of zinc sulfate are used in the inorganic fertilizer; 662.8 kg of organic fertilizer A and 50 kg of organic fertilizer B are used in the bio-organic fertilizer.

[0055] Table 1 Wrinkled papaya yield and base fertilizer application amount:

[0056] Group Types of base fertilizer Number of test plants Average fertilizer application rate Total output Average output Increase production control group Three-element compound fertilizer 10 strains 1.3kg / plant 295kg 29.5kg / plant Experimental group 1 Biological organic and inorganic fertilizers 10 strains 2.3kg / plant 300kg 30kg / plant 1.7％

[0057] In Junan County wrinkled papaya base test 4 years, in autumn respectively with ternary composite fertilizer (15-15-15) and the biological organic-inorganic fertilizer among the embodiment one as the base manure when wrinkled papaya saplings are planted for the first time, what test group one applied was the biological organic-inorganic fertilizer, and what the control group applied was the ternary composite fertilizer; Wherein, the average fertilizing amount of the ternary composite fertilizer was 1.3kg / strain, and the average fertilizing amount of the biological organic-inorganic fertilizer was 2.3kg / strain (the usage amount of the biological organic-inorganic fertilizer was different from the usage amount of the ternary composite fertilizer, but their prices were the same); Subsequently, two groups of 20 wrinkled papaya seedlings were applied with identical fertilizers; When the fourth year was gathered in the crops, the annual output of the test group one was 300kg, and the annual output of the control group was 295kg; The biological organic-inorganic fertilizer was that the wrinkled papaya of base manure increased production by 1.7% compared to the wrinkled papaya of base manure with the ternary composite fertilizer.

[0058] Example 2:

[0059] See also Figure 1 , a biological organic-inorganic fertilizer for wrinkled papaya, which is applied as a base fertilizer in the second and third years after the initial planting of wrinkled papaya seedlings;

[0060] The bio-organic-inorganic fertilizer comprises, by percentage, 62.6% bio-organic fertilizer, 37.26% inorganic fertilizer, 0.1% deep-tillage-free soil conditioner, 0.03% Bacillus subtilis A, and 0.01% Azospirillum; the bio-organic fertilizer comprises, by mass, 49 parts of organic fertilizer A and 13.6 parts of organic fertilizer B; the organic fertilizer A is made by fermenting chicken manure and cassava residue in a ratio of 7:3 under the condition of a composite bacterial agent of Thermophilic Sporotrichum YM-2; the organic fertilizer B is made by fermenting 100 parts of wrinkled papaya waste. The waste is fermented with a composite bacterial agent of Thermospora Sporotrichum YM-2 and Bacillus subtilis B; the inorganic fertilizer includes 136 parts of urea, 68 parts of monoammonium phosphate, 160 parts of potassium sulfate, and 8.6 parts of zinc sulfate in parts by mass; the ratio of N, P2O5 and K2O in the prepared bio-organic inorganic fertilizer is 7:3:8, the total mass fraction of N, P2O5 and K2O is ≥18%, the zinc content is ≥0.2%, the mass fraction of organic matter is ≥25%, and the density of Bacillus subtilis is ≥40 million / g.

[0061] To produce one ton of wrinkled papaya bio-organic-inorganic fertilizer, 626 kg of inorganic fertilizer, 372.6 kg of bio-organic fertilizer, 1 kg of deep-tillage-free soil conditioner, 0.3 kg of Bacillus subtilis A, and 0.1 kg of Azospirillum are needed; among them, 136 kg of urea, 68 kg of monoammonium phosphate, 160 kg of potassium sulfate, and 8.6 kg of zinc sulfate are used in the inorganic fertilizer; 490 kg of organic fertilizer A and 136 kg of organic fertilizer B are used in the bio-organic fertilizer.

[0062] Table 2 Wrinkled papaya yield and base fertilizer application amount:

[0063]

[0064]

[0065] In the four-year experiment at the wrinkled papaya base in Junan County, in the first year, in autumn, a triple compound fertilizer (15-15-15) and the biological organic-inorganic fertilizer in Example 1 were used as base fertilizers when the wrinkled papaya seedlings were first planted. The fertilizer application rates were: control group, triple compound fertilizer, 1.3 kg / plant; experimental group two, biological organic-inorganic fertilizer, 2.3 kg / plant. In the following year and the third year, the ternary compound fertilizer continued to be used as the base fertilizer for the control group, and the biological organic-inorganic fertilizer in Example 2 was used as the base fertilizer for the experimental group 2; each year, the average fertilization amount of the ternary compound fertilizer was 1.7 kg / plant, and the average fertilization amount of the biological organic-inorganic fertilizer was 2.9 kg / plant (the usage amount of the biological organic-inorganic fertilizer was different from that of the ternary compound fertilizer, but their prices were the same); subsequently, the same fertilizer was applied to a total of 20 wrinkled papaya seedlings in the two groups; at the time of the fourth year of harvest, the annual output of the experimental group 2 was 312 kg, and the annual output of the control group was 295 kg; the wrinkled papaya with the biological organic-inorganic fertilizer as the base fertilizer increased its yield by 5.8% compared to the wrinkled papaya with the ternary compound fertilizer as the base fertilizer.

[0066] Example 3:

[0067] See also Figure 1 , a biological organic-inorganic fertilizer for wrinkled papaya, which is applied as a base fertilizer in the second and third years after the initial planting of wrinkled papaya seedlings;

[0068] The bio-organic-inorganic fertilizer comprises, by percentage, 48.48% of bio-organic fertilizer, 51.38% of inorganic fertilizer, 0.1% of deep-tillage-free soil conditioner, 0.03% of Bacillus subtilis A, and 0.01% of Azospirillum. The bio-organic fertilizer comprises, by mass, 30.28 parts of organic fertilizer A and 18.2 parts of organic fertilizer B. The organic fertilizer A is made by fermenting chicken manure and cassava residue in a ratio of 7:3 under the condition of a composite bacterial agent of Thermophilic Sporotrichum YM-2. The organic fertilizer B is made by fermenting 100 parts of wrinkled papaya waste. The waste is fermented with a composite bacterial agent of Thermospora Sporotrichum YM-2 and Bacillus subtilis B; the inorganic fertilizer includes 196 parts of urea, 91 parts of monoammonium phosphate, 220 parts of potassium sulfate, and 6.8 parts of zinc sulfate in parts by mass; the ratio of N, P2O5 and K2O in the prepared bio-organic inorganic fertilizer is 10:4:11, the total mass fraction of N, P2O5 and K2O is ≥25%, the zinc content is ≥0.2%, the mass fraction of organic matter is ≥19%, and the density of Bacillus subtilis is ≥40 million / g.

[0069] To produce one ton of wrinkled papaya bio-organic-inorganic fertilizer, 484.8 kg of inorganic fertilizer, 513.8 kg of bio-organic fertilizer, 1 kg of deep-tillage-free soil conditioner, 0.3 kg of Bacillus subtilis A, and 0.1 kg of Azospirillum are needed; among them, 196 kg of urea, 91 kg of monoammonium phosphate, 220 kg of potassium sulfate, and 6.8 kg of zinc sulfate are used in the inorganic fertilizer; 302.8 kg of organic fertilizer A and 182 kg of organic fertilizer B are used in the bio-organic fertilizer.

[0070] Table 3 Wrinkled papaya yield and base fertilizer application amount:

[0071] Group Types of base fertilizer Number of test plants Average fertilizer application rate Total output Average output Increase production control group Three-element compound fertilizer 10 strains 1.9kg / plant 295kg 29.5kg / plant Experimental group three Biological organic and inorganic fertilizers 10 strains 3kg / plant 320kg 32kg / plant 8.5％

[0072] In Junan County wrinkled papaya base test 4 years, in the first year respectively in autumn with ternary compound fertilizer (15-15-15) and the biological organic-inorganic fertilizer in Example 1 as the base manure when wrinkled papaya saplings are first planted, fertilizer amount: control group, ternary compound fertilizer, 1.3kg / strain; test group three, biological organic-inorganic fertilizer, 2.3kg / strain. In the next year and the third year, ternary compound fertilizer (15-15-15) and the biological organic-inorganic fertilizer in Example 2 are used as the base manure, fertilizer amount: control group, ternary compound fertilizer, 1.7kg / strain; test group three, biological organic-inorganic fertilizer, 2.9kg / strain. In the fourth year, a 15-15-15 nutrient fertilizer and the bio-organic-inorganic fertilizer from Example 3 were used as base fertilizers. The control group received 1.9 kg of nutrient fertilizer per plant, while the experimental group received 3 kg of bio-organic-inorganic fertilizer per plant (the amount of bio-organic-inorganic fertilizer differed from that of the 15-15-15 nutrient fertilizer, but their prices were the same). Subsequently, the same fertilizer was applied to 40 wrinkled papaya seedlings from both groups. After harvest, the wrinkled papaya seedlings that received the bio-organic-inorganic fertilizer as a base fertilizer had an 8.5% yield increase compared to the 15-15-15 nutrient fertilizer.

[0073] In Example 1 to Example 3, the addition of the ternary compound fertilizer in the control group is the most suitable fertilization amount, and adding the fertilization amount of the ternary compound fertilizer again will not increase yield, and the fertilization amount of the biological organic-inorganic fertilizer is just the equivalent fertilization amount of the ternary compound fertilizer, and when improving the application cost of the biological organic-inorganic fertilizer, that is, the fertilization amount, the yield of wrinkled papaya can also be suitably increased;

[0074] Table 4 Wrinkled papaya yield and base fertilizer application amount:

[0075] Group Types of base fertilizer Number of test plants Average fertilizer application rate Total output Average output Increase production Experimental group 4 Biological organic and inorganic fertilizers 10 strains 2.5kg / plant 310kg 31kg / plant 3.3％ Experimental group 5 Biological organic and inorganic fertilizers 10 strains 3.1kg / plant 321kg 32.1kg / plant 2.9％ Experimental group 6 Biological organic and inorganic fertilizers 10 strains 3.2kg / plant 335kg 33.5kg / plant 4.7％

[0076] In the fourth test group, the amount of the biological organic-inorganic fertilizer applied in Example 1 was increased under the conditions of the first test group, and the original 2.3 kg / plant was adjusted to 2.5 kg / plant. At the time of harvest, the yield of the wrinkled papaya was increased by 3.3% compared with that of the first test group; in the fifth test group, the amount of the biological organic-inorganic fertilizer applied in Example 2 was increased under the conditions of the second test group, and the original 2.9 kg / plant was adjusted to 3.1 kg / plant. At the time of harvest, the yield of the wrinkled papaya was increased by 2.9% compared with that of the second test group; in the sixth test group, the amount of the biological organic-inorganic fertilizer applied in Example 3 was increased under the conditions of the third test group, and the original 3 kg / plant was adjusted to 3.2 kg / plant. At the time of harvest, the yield of the wrinkled papaya was increased by 4.7% compared with that of the third test group.

[0077] The preparation method of wrinkled papaya biological organic and inorganic fertilizer is as follows:

[0078] Step 1: Preparation of organic fertilizer A:

[0079] A1. Test the total moisture content of chicken manure and cassava residue (chicken manure and cassava residue are mixed in a ratio of 7:3) to ensure that the total moisture content of chicken manure and cassava residue is between 55% and 65%. When the moisture content of chicken manure and cassava residue is higher than 65%, dehydrate the chicken manure and cassava residue, and store the wastewater after dehydration. The wastewater can be used for fermentation to produce biogas, or for humidifying chicken manure and cassava residue with a moisture content below 55%. Then, store the wastewater between 55% and 65% The chicken manure and cassava residue are transported to the livestock and poultry manure harmless treatment equipment in a ratio of 7:3. The livestock and poultry manure harmless treatment equipment heats the internal temperature of the livestock and poultry manure harmless treatment equipment to 65°C by igniting the heating system. By heating the chicken manure and cassava residue, not only can the pathogens in the chicken manure and cassava residue be disinfected, but the moisture in the chicken manure and cassava residue can also be reduced. The heating ends after 1 hour. At this time, the moisture content in the chicken manure and cassava residue is 45% to 50%, which is suitable for fermentation and composting.

[0080] A2. After the ignition and heating treatment is completed, the heating temperature of the livestock and poultry manure harmless treatment equipment is quickly reduced to 50° C., and then the composite bacterial agent of Thermophilic Sporotrichum YM-2 is evenly sprayed on the chicken manure and cassava residue. The spraying amount of the composite bacterial agent of Thermophilic Sporotrichum YM-2 is approximately 0.1% of the total weight of the chicken manure and cassava residue. While spraying Thermophilic Sporotrichum YM-2, the chicken manure and cassava residue are turned over. By turning over the chicken manure and cassava residue, not only can the chicken manure and cassava residue be mixed, but also the composite bacterial agent of Thermophilic Sporotrichum YM-2 can be fully contacted and mixed with the chicken manure and cassava residue; under the condition of 50° C., the composite bacterial agent of Thermophilic Sporotrichum YM-2 is evenly sprayed on the chicken manure and cassava residue. Providing nitrogen and carbon sources to the composite bacterial agent of Thermophilic Sporotrichum YM-2 allows the bacteria in the composite bacterial agent of Thermophilic Sporotrichum YM-2 to multiply rapidly. The composite bacterial agent of Thermophilic Sporotrichum YM-2 can decompose macromolecular nutrients such as cellulose into small molecular nutrients, thereby increasing the composting speed and reducing the composting cycle. At the same time, it can also quickly degrade the sulfur-containing compounds and nitrogen-containing compounds that produce odor in organic materials such as livestock and poultry manure; then, an appropriate amount of air is introduced into the interior of the livestock and poultry manure harmless treatment equipment. The entry of air will cause the temperature inside the livestock and poultry manure harmless treatment equipment to be slightly lower than 50°C, thereby being more suitable for the composite bacterial agent of Thermophilic Sporotrichum YM-2; then, the chicken manure and cassava residue are fermented and composted;

[0081] A3. After 1 hour, the degree of maturity of chicken manure and cassava residue can reach 60%. At this time, the chicken manure and cassava residue after preliminary decomposition are exported to the harmless treatment equipment for livestock and poultry manure and subjected to secondary composting fermentation. After 10 to 15 days, the chicken manure and cassava residue will be completely decomposed. The decomposed chicken manure and cassava residue mixture is organic fertilizer A, and the organic matter content in organic fertilizer A is greater than or equal to 40.5%; Organic fertilizer A is then packaged and stored for standby use; Adding a composite bacterial agent of Thermophilic Sporotrichum YM-2 during the decomposition process of chicken manure and cassava residue can increase the decomposition rate of organic matter, thereby increasing the content of small molecular organic matter in organic fertilizer A, and then increasing the content of organic colloid in organic fertilizer A;

[0082] Step 2: Preparation of organic fertilizer B:

[0083] B1, when wrinkled papaya is processed, can be by wrinkled papaya wastes such as melon peel and bad melon, these wrinkled papaya wastes are collected, then the wrinkled papaya waste is sterilized, subsequently the wrinkled papaya waste after the sterilization is transported in the fermentation tank, and the composite microbial agent of thermophilic Sporotrichum syringae YM-2 is sprayed and fermented, and the weight of the composite microbial agent of thermophilic Sporotrichum syringae YM-2 is approximately 0.1% of the wrinkled papaya waste;

[0084] B2, after 45min, the wrinkled papaya waste degree of decomposition reaches 60%, then in fermentor tank, import air and fermentor tank is cooled, after the temperature of fermentor tank is reduced to normal temperature, bacillus subtilis B is sprayed on the wrinkled papaya waste in fermentor tank and continues fermentation, bacillus subtilis B adopts bacillus subtilis HD11, after 10~12 days, the wrinkled papaya waste can be fully decomposed, and the wrinkled papaya waste after decomposition is exactly organic fertilizer B, and organic matter content is more than or equal to 40.5% in organic fertilizer B; Subsequently organic fertilizer B is packed and stored, and leaves for standby; The composite microbial agent of thermophilic siderophore YM-2 can decompose macromolecular organic matter into small molecule organic matter in the wrinkled papaya waste in fermentation, and bacillus subtilis HD11 can generate gamma-polyglutamic acid in fermentation, and gamma-polyglutamic acid can chelate with calcium ion and iron ion, thereby delays the generation of calcium phosphate precipitation;

[0085] Step 3: Preparation of biological organic and inorganic fertilizers:

[0086] C1. Grinding urea, monoammonium phosphate, potassium sulfate, and zinc sulfate separately to obtain urea, monoammonium phosphate, potassium sulfate, and zinc sulfate powders, and storing the urea, monoammonium phosphate, potassium sulfate, and zinc sulfate powders separately;

[0087] C2. Mixing urea powder, zinc sulfate powder, and a no-deep-tillage soil conditioner according to the proportions in the embodiment, stirring, and fully mixing the urea powder, zinc sulfate powder, and the no-deep-tillage soil conditioner to obtain a mixture A. The no-deep-tillage soil conditioner is Agri-sc no-deep-tillage soil conditioner;

[0088] The monoammonium phosphate powder and the organic fertilizer B are mixed and stirred according to the ratio in the embodiment, and the monoammonium phosphate powder and the organic fertilizer B are fully mixed to obtain a mixture B; the gamma-polyglutamic acid in the organic fertilizer B can protect the monoammonium phosphate. When in contact with calcium and iron ions, the gamma-polyglutamic acid first chelates with the calcium and iron ions, thereby preventing the free phosphorus from crystallizing, thereby ensuring that the phosphorus can be effectively absorbed by the wrinkled papaya;

[0089] Potassium sulfate powder and organic fertilizer A are mixed and stirred according to the ratio in the embodiment. After the potassium sulfate powder and organic fertilizer A are fully mixed, a mixture C is obtained; the organic colloid in the organic fertilizer A can absorb the potassium ions in the potassium sulfate powder, and after fertilization, the loss of potassium ions can be slowed down, thereby improving the application effect of potash fertilizer;

[0090] C3, first mixture A is fully mixed with mixture C and Azospirillum according to the ratio in the embodiment, and Azospirillum is used for nitrogen fixation, thereby increasing the nitrogen content in the fertilizer; and then mixture B and Bacillus subtilis A are added and fully mixed according to the ratio in the embodiment to obtain a bio-organic-inorganic fertilizer semi-finished product; the chicken manure of mixture C contains more calcium ions, and monoammonium phosphate is first mixed with organic fertilizer B, and then mixed with mixture C, which can effectively protect phosphoric acid; simultaneously, the organic acid in the organic fertilizer can make the pH of the soil weakly acidic at 6-7, which is also suitable for the planting of wrinkled papaya under this condition; under this condition, when planting wrinkled papaya, only calcium ions and iron ions can make phosphoric acid crystallize, and gamma-polyglutamic acid is utilized to avoid the crystallization of phosphoric acid, thereby improving the utilization rate of phosphate fertilizer; Bacillus subtilis A adopts Bacillus subtilis HG-15, and the density of Bacillus subtilis A in the bio-organic-inorganic fertilizer is greater than or equal to 400 million / gram;

[0091] C4. Granulate and pack the semi-finished bio-organic and inorganic fertilizers to produce bio-organic and inorganic fertilizers.

[0092] The basic principles, main features and advantages of the present invention are shown and described above. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is limited by the appended claims rather than the above description. Therefore, it is intended that all changes that fall within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any figure signs in the claims should not be regarded as limiting the claims involved.

[0093] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a wrinkled papaya biological organic and inorganic fertilizer, characterized in that, Included by percentage; 48.48% to 71.28% of bio-organic fertilizer, wherein the bio-organic fertilizer comprises 30.28 to 66.28 parts of organic fertilizer A and 5 to 18.2 parts of organic fertilizer B by weight; 28.58% to 51.38% of inorganic fertilizer; the inorganic fertilizer includes, by mass, 124 to 196 parts of urea, 25 to 91 parts of monoammonium phosphate, 130 to 220 parts of potassium sulfate, and 6.8 to 8.6 parts of zinc sulfate; No-deep-tillage soil conditioner 0.1%; Bacillus subtilis A 0.03%; Azospirillum 0.01%; The organic fertilizer A comprises a composite bacterial agent of chicken manure, cassava residue and Thermospora distachyon YM-2; The organic fertilizer B comprises wrinkled papaya waste, a composite bacterial agent of Thermospora spp. YM-2 and Bacillus subtilis B; The preparation method of the wrinkled papaya biological organic-inorganic fertilizer is as follows: Step 1: Preparation of organic fertilizer A: A1. Transfer chicken manure and cassava residue in a ratio of 7:3 to a livestock and poultry manure harmless treatment device. Heat the device to 65°C to sterilize the chicken manure and cassava residue. A2. Lower the heating temperature of the livestock and poultry manure harmless treatment equipment to 50°C, then evenly spray the Thermophilic Sporotrichum YM-2 compound inoculant onto the chicken manure and cassava residue, and then ferment and compost the chicken manure and cassava residue. The spraying rate of the Thermophilic Sporotrichum YM-2 compound inoculant is 0.1% of the total weight of the chicken manure and cassava residue. A3. After the chicken manure and cassava residue have reached 60% maturity, remove them and compost them until they are fully mature, thereby obtaining organic fertilizer A. Step 2: Preparation of organic fertilizer B: B1, 100 parts of wrinkled papaya wastes were transported to a fermentation tank and sprayed with a composite bacterial agent of 0.1 part of Thermophilic Sporotrichum spp. YM-2 for fermentation; B2, after the wrinkled papaya waste maturity reached 60%, sprayed 0.01 part of Bacillus subtilis B to the wrinkled papaya waste and continued to ferment, until the wrinkled papaya waste was fully mature, organic fertilizer B can be obtained; Described Bacillus subtilis B adopts Bacillus subtilis HD11; Step 3: Preparation of biological organic and inorganic fertilizers: C1. Grinding urea, monoammonium phosphate, potassium sulfate, and zinc sulfate respectively to obtain urea, monoammonium phosphate, potassium sulfate, and zinc sulfate powders; C2. Fully mix urea powder, zinc sulfate powder, and a soil conditioner that does not require deep tillage to obtain a mixture A; fully mix monoammonium phosphate powder and organic fertilizer B to obtain a mixture B; and fully mix potassium sulfate powder and organic fertilizer A to obtain a mixture C. C3. First, thoroughly mix mixture A, mixture C, and Azospirillum, and then add mixture B and Bacillus subtilis A and mix thoroughly to obtain a semi-finished bio-organic fertilizer; the Bacillus subtilis A is Bacillus subtilis HG-15; C4. Granulate and pack the semi-finished bio-organic and inorganic fertilizers to produce bio-organic and inorganic fertilizers.

2. the preparation method of a kind of wrinkled papaya biological organic-inorganic fertilizer according to claim 1, is characterized in that: In the step 1, before mixing the chicken manure and cassava residue, the water content of the chicken manure and cassava residue is first detected to ensure that the water content of the chicken manure and cassava residue is between 55% and 65%; When the water content is higher than 65%, the chicken manure and cassava residue are dehydrated and the sewage is retained; when the water content is lower than 55%, the separated sewage or tap water is used to replenish the chicken manure and cassava residue.

3. the preparation method of a kind of wrinkled papaya biological organic-inorganic fertilizer according to claim 1, is characterized in that: In the steps 1 and 2, when spraying the composite bacterial agent of Thermospora Sporotrichum YM-2, the chicken manure, cassava residue and wrinkled papaya waste are turned over.

4. the preparation method of a kind of wrinkled papaya biological organic-inorganic fertilizer according to claim 1, is characterized in that: In the step 2, when spraying Bacillus subtilis B, the temperature of the fermentation tank is first lowered to room temperature, and then Bacillus subtilis B is sprayed. After spraying Bacillus subtilis B, the temperature of the fermentation tank is raised to 40°C.

5. A wrinkled papaya biological organic-inorganic fertilizer, characterized in that: The method for preparing the wrinkled papaya biological organic and inorganic fertilizer according to any one of claims 1 to 4 is used to prepare the biological organic and inorganic fertilizer; The density of Bacillus subtilis A in the bio-organic-inorganic fertilizer is greater than or equal to 40 million / gram.

6. A wrinkled papaya biological organic-inorganic fertilizer according to claim 5, characterized in that: The bio-organic-inorganic fertilizer contains 71.28% bio-organic fertilizer; The bio-organic fertilizer comprises, by mass, 66.28 parts of organic fertilizer A and 5 parts of organic fertilizer B; The inorganic fertilizer includes 124 parts of urea, 25 parts of monoammonium phosphate, 130 parts of potassium sulfate, and 6.8 parts of zinc sulfate in parts by mass.

7. A wrinkled papaya biological organic-inorganic fertilizer according to claim 5, characterized in that: The bio-organic-inorganic fertilizer contains 62.6% bio-organic fertilizer; The bio-organic fertilizer comprises, by mass, 49 parts of organic fertilizer A and 13.6 parts of organic fertilizer B; The inorganic fertilizer includes 136 parts of urea, 68 parts of monoammonium phosphate, 160 parts of potassium sulfate, and 8.6 parts of zinc sulfate in parts by mass.

8. A wrinkled papaya biological organic-inorganic fertilizer according to claim 5, characterized in that: The bio-organic-inorganic fertilizer contains 48.48% bio-organic fertilizer; The bio-organic fertilizer comprises, by mass, 30.28 parts of organic fertilizer A and 18.2 parts of organic fertilizer B; The inorganic fertilizer includes 196 parts of urea, 91 parts of monoammonium phosphate, 220 parts of potassium sulfate, and 6.8 parts of zinc sulfate in parts by mass.

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