An indoor artificial feeding method of Bradybaena similaris

Abstract

The application discloses an indoor artificial feeding method of Bradybaena similaris, which comprises the following steps: (1) preparation of food for a feeding box; (2) feeding and daily management of the Bradybaena similaris; (3) after the Bradybaena similaris develops to a seed snail stage, the Bradybaena similaris in the seed snail stage is put into the feeding box until the Bradybaena similaris lays eggs; and (4) selection and hatching of the eggs of the Bradybaena similaris after the Bradybaena similaris lays the eggs. The method is simple in operation, free of ecological pollution in breeding, low in feeding cost and labor input, solves the problem of large-scale breeding of the Bradybaena similaris, can be massively bred indoors, is easy to popularize and has high economic value.

Description

Technical Field

[0001] This invention relates to a method for indoor artificial rearing of the Chinese snail. Background Technology

[0002] Chinese snail ( Cathaica fasciola It belongs to the class Gastropoda, order Stylommatophora, family Barnacleidae. Bradybaenidae Barnacles are mollusks belonging to the genus *Barnacleus*. According to relevant literature, there are 80 genera of barnacles, distributed across tropical and temperate continents and islands. Barnacles have been found in various regions, including Asia, Africa, Europe, North America, and South America, with Plutitic fossils discovered in Europe. Barnacles are widely distributed in China, found in all provinces and cities. Three main species of barnacles exist in my country: *Barnacleus stripeus*, *Barnacleus grayi*, and *Barnacleus homotypicus*.

[0003] The striped snail is an omnivorous mollusc that damages agriculture and horticulture, feeding on the young shoots and tender leaves of crops such as cotton, hemp, wheat, tobacco, vegetables, and fruits. It is mainly distributed in Beijing, Shaanxi, Shanxi, Hunan, Jiangsu, Hebei, and Henan provinces of China. Striped snails generally inhabit damp grassy areas, bushes, under rocks, or in crevices near hillsides, farmland, livestock sheds, and parks. They reproduce once or twice a year, laying 100-200 eggs, about the size of a mung bean, white, and oval, often found under rocks, in soil, and in soil crevices. The shell is medium-sized, slightly thick, solid, and dull, resembling a short cone, with five whorls, and is yellow or yellowish-brown. Individuals living in cold climates exhibit obvious hibernation, typically developing a white operculum to seal the shell opening. A parasite called *Diplocolumnares lanceolatum* can infest striped snails. Dicrocoelium lanceatum The parasite, which can cause zoonotic clostridial disease, also known as bile duct fibrosis, can lead to death from extreme exhaustion.

[0004] Existing methods for controlling striped snails include physical and chemical control. Physical control methods are time-consuming and labor-intensive, and are generally used as supplementary measures. While chemical control is effective, it easily leads to drug resistance and causes serious environmental pollution. Therefore, developing a green control system for striped snails has become a key research direction. Obtaining large-scale indoor breeding samples of striped snails can better provide material support for food control research. Currently, snail farming focuses primarily on white jade snails, with little attention paid to agricultural snails in my country. Therefore, experimental samples of striped snails are mainly collected from the wild. However, relying solely on wild collection wastes significant human, material, and financial resources, which is detrimental to research on striped snails and may disrupt the ecological balance of the collection sites. Therefore, establishing an indoor artificial breeding method for the striped snail, through indoor rearing of the snail eggs and juvenile snails, is beneficial for observing and understanding the snail's life characteristics, behavioral habits, feeding preferences, optimal temperature and humidity environment, spatial distribution patterns, and other environmental conditions during its growth and development from egg to juvenile to adult and then to breeding stage. This can provide sufficient experimental materials for scientific research related to the striped snail, and at the same time promote the development of green striped snail control technologies, which has important scientific, economic, and ecological value. Summary of the Invention

[0005] This invention provides an indoor artificial breeding method for *Sinocyclocheilus fasciatus*, solving the difficulty of large-scale breeding. This method allows for the breeding of juvenile *Sinocyclocheilus fasciatus* throughout its life cycle, from hatching to egg production, enabling continuous indoor breeding. Furthermore, it provides sufficient and reliable experimental data for subsequent research on the life tables and molecular biology of *Sinocyclocheilus fasciatus*, possessing significant scientific, economic, and ecological value. The method also boasts advantages such as low breeding costs, minimal labor input, pollution-free breeding environment, and simple operation.

[0006] The method for indoor artificial breeding of the Chinese snail according to the present invention is as follows:

[0007] (1) Use an open hard box as a feeding box. After disinfecting the feeding box, lay a 10-15cm thick layer of disinfected, pollution-free sandy soil at the bottom of the box. Then use dechlorinated water to adjust the soil moisture to 80%-90%. Mix 70-75% of the weight of disinfected cruciferous and / or cucurbitaceous plant leaves and / or tender buds, 1% of the weight of disinfected eggshells, and 24-29% of the weight of snail concentrate feed as feed.

[0008] The unpolluted sandy soil is sandy soil without any planting background, and the disinfection is carried out by sun exposure for 3-5 days; the cruciferous plants are one or more of Chinese cabbage, cauliflower, radish, mustard greens, and kale, and one or more of Cucurbitaceae plants are loofah, cucumber, pumpkin, bitter melon, and winter melon.

[0009] (2) Put the Bradybaena similaris on the sandy soil in the feeding box, and set up an insect-proof net on the open top of the feeding box;

[0010] The insect-proof net is 20-25 mesh;

[0011] (3) Put the feeding box into a feeding room with a temperature of 25-27℃ and a relative humidity of 70%-80%, and use a daylight lamp to provide light in the feeding. The day and night alternating cycle is 12 hours of light and 12 hours of darkness. Feed at 8 am every day, and feed the food every 24 hours. After 24 hours of feeding, observe and record the feeding condition of Bradybaena similaris and clean up the food residue to prevent food from rotting and breeding bacteria. Replace the sandy soil every 7 days, and adjust the number of Bradybaena similaris and the amount of food in each feeding box as the age increases. Monitor the relative humidity and soil humidity in the room and supplement water in time;

[0012] When the area of the bottom of the feeding box is 0.22-0.25m 2 , and the height is 15-20cm, place 800 Bradybaena similaris in each feeding box during the juvenile period, and the amount of food in each feeding box is 16.8-24.0g / day; place 750-770 Bradybaena similaris in each feeding box at one month old, and the amount of food in each feeding box is 22.8-26.7g / day; place 690-720 Bradybaena similaris in each feeding box at two months old, and the amount of food in each feeding box is 24.3-28.7g / day; place 590-620 Bradybaena similaris in each feeding box at three months old, and the amount of food in each feeding box is 31.3-36.1g / day; place 560-580 Bradybaena similaris in each feeding box at four months old, and the amount of food in each feeding box is 34.8-37.7g / day; place 460-500 Bradybaena similaris in each feeding box at five months old, and the amount of food in each feeding box is 33.6-36.7g / day; place 380-420 Bradybaena similaris in each feeding box during the breeding period, and the amount of food in each feeding box is 32.3-34.1g / day;

[0013] Replace 1 / 2 of the soil in the feeding box every 7 days and adjust the soil humidity;

[0014] (4) After Bradybaena similaris develops to the breeding period, place the Bradybaena similaris in the breeding period into a new feeding box. First, place 8-12cm of sterilized sandy soil in the feeding box, then place 3-5cm of sterilized loam, and finally place a layer of moist green moss. Put the Bradybaena similaris in the breeding period into the feeding box until the Bradybaena similaris lays eggs;

[0015] (5) carefully take out the ovum of Bradybaena ravida from the rearing box, select qualified ovum of Bradybaena ravida, and then place the ovum into a new rearing box in which sterile sandy soil is paved, cover the ovum with 0.8-1.2cm thick sandy soil, wet the ovum with spray-shaped chlorine-free water, the soil surface humidity is 25%-35%, place the ovum at 28℃, use a daylight lamp to provide light, the day and night alternating cycle is 9h light and 15h dark, monitor the soil surface humidity and supplement water in time, after the ovum hatches, transfer the Bradybaena ravida in the juvenile stage into a new rearing box and continue to cultivate according to the method of steps (2)-(4);

[0016] The selection of the qualified ovum of Bradybaena ravida is to spray the surface of the ovum of Bradybaena ravida with alcohol with a mass concentration of 75%, rinse with sterile water, and then carefully place the ovum into a sterile culture dish in which sterile wet filter paper is paved at the bottom, and select the ovum of Bradybaena ravida which is oval, white, has an intact mucous membrane protective layer, and is similar in size to a green bean with sterile tweezers.

[0017] Check whether the indoor relative humidity and soil humidity meet the survival standard of Bradybaena ravida every 4 hours.

[0018] Advantages and technical effects of the present application:

[0019] (1) In the past, most of the collection and life history researches of Bradybaena ravida are collected outdoors or in the wild, only a few records of the indoor rearing method of Bradybaena ravida are not systematic, and it is difficult to obtain cheap samples required by experiments in large quantities, and it is also difficult to obtain the life table of Bradybaena ravida, the indoor rearing method of Bradybaena ravida adopted in the present application not only solves the problem of non-systematic rearing of Bradybaena ravida, but also is easy to observe the habits of Bradybaena ravida in the growth period; the indoor rearing method of the present application simulates the survival environment of Bradybaena ravida according to the biological learning of Bradybaena ravida, provides activity space for the activity of Bradybaena ravida, can guarantee the normal growth and development of Bradybaena ravida indoors, and effectively solves the practical difficulties of Bradybaena ravida which cannot be mass-reared;

[0020] (2) The water used for spraying and humidifying in the present application is chlorine-free water obtained by filtration, the surface humidity of the ovum of Bradybaena ravida is monitored every day, and water is sprayed and supplemented in time, the existence of the plastic greenhouse guarantees the stability of the temperature of the living environment of Bradybaena ravida, which is conducive to the growth of Bradybaena ravida and the hatching of the ovum, maintains the relative humidity of the environment in the greenhouse, prolongs the fresh-keeping time of the food of Bradybaena ravida, and guarantees the normal hatching of the ovum of Bradybaena ravida;

[0021] (3) the indoor feeding method of the application uses the feeding box and foodstuff, and the surface is disinfected by 75% alcohol disinfectant every day, and the soil in the feeding box of each age period of Bradybaena fruticum is replaced every 7 days, and the soil humidity is adjusted by spraying chlorine-free water, the foodstuff is fed every 24 hours, and the food residue is cleaned, the relative humidity and soil humidity are checked every 4 hours, whether it meets the survival standard of Bradybaena fruticum, effectively avoiding the occurrence of snail mass disease, guaranteeing the indoor continuous culture of Bradybaena fruticum, and reducing the difficulty of artificial management;

[0022] (4) the application solves the problems of Bradybaena fruticum climbing, insufficient utilization of foodstuff, and chaotic management, the feeding box has simple structure, low feeding cost, and less labor input, meets the needs of the growth of Bradybaena fruticum, and is suitable for the feeding of Bradybaena fruticum in each development period;

[0023] (5) the feeding method of the application can continuously culture Bradybaena fruticum indoors, and the application has the advantages of simple operation, pollution-free ecological breeding, low feeding cost, and less labor input, and solves the problem of large-scale breeding of Bradybaena fruticum. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme and advantages of the embodiments of the application clearer, the technical scheme of the embodiments of the application will be clearly and completely explained below. The following described embodiments are only some of the embodiments of the application, not all the embodiments. Unless otherwise specified, the example materials used in the following embodiments are commercially available products.

[0025] The shape and size of the feeding box are not limited in the application, which can be square or circular, large or small. The material of the feeding box is also not limited in the application, which can be wood or plastic. The size and material of the feeding box are selected according to the feeding amount and actual situation, but the feeding box must have air permeability and light permeability. Embodiment 1

[0026] (1) a ventilated and clean house is selected as a feeding room, which is cleaned and disinfected with 84 disinfectant, and a breeding rack is built after 1 hour, the breeding rack is arranged with a water pipe, and a plurality of spray heads are arranged on the water pipe for soil moisture retention;

[0027] The embodiment adopts an open rectangular plastic box as a rearing box, uses a 25-mesh insect-proof net and a rubber strip to make a box cover, the rearing box has a size of 60 cm x 40 cm x 18 cm, and the rearing box is suitable for breeding of the Megalobulimus lineatus in the juvenile snail stage to the adult snail stage. After the rearing box is disinfected by using 75% alcohol or 84 disinfectant, the rearing box is placed for 2-3 days, 10-15 cm of disinfected and unpolluted sandy soil is laid on the bottom of the rearing box, and then dechlorinated water is used to adjust the soil humidity to 80%-90%. The sandy soil is collected from a small soil slope in the wild, is artificially collected, is disinfected by using the sun for 5 days, during which large impurities and stones are screened out, and is prepared for use.

[0028] The Megalobulimus lineatus food: 75% of cabbage leaves (disinfected by using 75% alcohol, and then cut into sections by using a leaf cutter), 1% of eggshells disinfected by using 75% alcohol, and 24% of snail concentrated feed are mixed, and water is sprayed to wet the mixture. The eggshells supplement calcium required by the snails, prevent the snails from having a shedding disease, and ensure normal growth and development of the Megalobulimus lineatus.

[0029] (2) The Megalobulimus lineatus is collected from farmland soil in Zichuan County, Nanyang City, Henan Province, and the Megalobulimus lineatus in the juvenile snail stage collected in the wild is transferred to the rearing box containing food by using disinfected tweezers, and the insect-proof net is covered.

[0030] (3) The rearing box is placed on a breeding rack in a rearing room with a temperature of 26°C, a temperature variation of not more than 1°C, and a relative humidity of 75%-80%, a spray head is arranged above each rearing box to keep the soil moist, an ultrasonic atomizing humidifier and a warm air blower are arranged in the rearing room to ensure stability of the temperature and humidity in the rearing room, a temperature control variable frequency negative pressure blower is regularly started to ensure air circulation in the rearing room, the temperature control variable frequency negative pressure blower is started for 1-2 hours every day, a daylight lamp is used to provide light, the snails cannot be irradiated by strong light, therefore, the snails are irradiated by using scattered light, and a day and night alternating cycle is 12 hours of light and 12 hours of darkness.

[0031] Food is fed at 8 o'clock every day, the food is fed once every 24 hours, the feeding condition of the Megalobulimus lineatus is observed and recorded, and food residues are cleaned up 24 hours after the food is fed, the sandy soil in the rearing box is replaced once every 7 days, the number of the Megalobulimus lineatus and the amount of food in each rearing box are adjusted as the age increases, and the relative humidity and the soil humidity in the room are checked every 4 hours, and water is supplemented in time.

[0032] The 800 snails of the juvenile snail stage are placed in each feeding box, and the amount of food in each feeding box is 20g / day; the 760 snails of the 1-month-old snail stage are placed in each feeding box, and the amount of food in each feeding box is 23g / day, the 700 snails of the 2-month-old snail stage are placed in each feeding box, and the amount of food in each feeding box is 25g / day; the 600 snails of the 3-month-old snail stage are placed in each feeding box, and the amount of food in each feeding box is 34.5g / day; the 580 snails of the 4-month-old snail stage are placed in each feeding box, and the amount of food in each feeding box is 35g / day; the 480 snails of the 5-month-old snail stage are placed in each feeding box, and the amount of food in each feeding box is 35g / day; the 400 snails of the seed snail stage (6-month-old snail stage) are placed in each feeding box, and the amount of food in each feeding box is 33g / day; when the food is insufficient or excessive, the food can be appropriately added or reduced;

[0033] (4) After the snail reaches the seed snail stage, the snail of the seed snail stage is placed in a new feeding box, 10cm of sterilized sandy soil is first placed in the feeding box, then 5cm of sterilized loam is placed, and finally a layer of moist moss is placed; 400 snails are placed in each feeding box until the snail lays eggs;

[0034] (5) A 9cm-diameter circular culture dish, filter paper and ultrapure water are sterilized, the snail eggs are carefully taken out from the feeding box, the surface of the snail eggs is sprayed and disinfected by using 75% alcohol disinfectant, and then the snail eggs are rinsed with sterile ultrapure water, and then the snail eggs are carefully placed in a sterile culture dish with sterilized moist filter paper at the bottom, the oval, white, complete mucous membrane protective layer, and green bean-sized snail eggs are selected by using a sterile forceps, 200 snail eggs are obtained, and then the selected snail eggs are placed in a new feeding box in a single separation mode, the surface of the snail eggs is covered with 1cm-thick sandy soil, the surface of the soil is moistened by using spray-shaped chlorine-free water, the surface humidity of the soil is 25%-30%, the culture dish is placed in a 28℃ environment, light is provided by using a daylight lamp, the day and night alternating cycle is 9h of light and 15h of darkness, the surface humidity of the soil is observed every two hours, and water is supplemented in time to keep the surface humidity of the snail eggs appropriate; after the snail eggs are hatched, the juvenile snail is transferred into a new feeding box, and the culture is continued according to the methods in steps (2)-(4);

[0035] Table 1 Average survival rate of snails at each stage (three replicates, average survival rate)

[0036]

[0037] The optimization process of the key points of the application is as follows:

[0038] 1. Selection of optimal temperature for rearing: The constant temperature was set at (20±1)℃, (22±1)℃, (24±1)℃, (26±1)℃, (28±1)℃, and the variable temperature was set at 28℃ during the day and 22℃ at night. The results showed that the growth condition was optimal and the survival rate was best at (26±1)℃.

[0039] 2. Selection of optimal humidity for rearing: The temperature was set at 26℃, and the humidity was set at (65±5)%, (75±5)%, and (85±5)%, respectively. The results showed that the growth condition and survival rate of the snails were best at (75±5)%.

[0040] 3. Selection of optimal photoperiod for rearing: The temperature was set at 26℃, the humidity was set at (75±5)%, and the photoperiod was set at L:D=16:8, L:D=13.5:10.5, L:D=20:4, and L:D=12:1, respectively. The results showed that the survival rate was highest at L:D=12:12.

[0041] Example 2: The method of this example is the same as that of Example 1, except that the floor of the rearing room is made of sterilized bricks with a height of 18 cm and an area of 4 m 2 of square grid, and 10-15 cm thick sterilized and unpolluted sandy soil is laid in the square grid. The food of the snails is a mixture of 70% of squash and gourd leaves and sprouts (sterilized with 75% alcohol and cut into sections using a leaf cutter), 1% of eggshells sterilized with 75% alcohol, and 29% of snail concentrate feed.

[0042] The number of snails to be released in each stage in the rearing box of this example is as follows: 13,300 for the juvenile snails, 12,600 for the one-month-old snails, 11,600 for the two-month-old snails, 10,000 for the three-month-old snails, 9,600 for the four-month-old snails, 8,000 for the five-month-old snails, 6,600 for the six-month-old snails (breeding snails), and 3,300 for the snail eggs. The survival rate of the snails in each stage is shown in the following table.

[0043] Table 2 Average survival rate of snails in each stage

[0044] .

Claims

1. A method for indoor artificial breeding of Bradybaena similaris, characterized in that, The steps are as follows: (1) using an open hard box as a feeding box, after disinfecting the feeding box, laying 10-15 cm thick disinfection non-polluted sandy soil on the bottom of the box, then using dechlorinated water to adjust the soil humidity to 80%-90%, mixing the disinfection leaves or / and sprouts of the cruciferous and / or cucurbitaceae plants with the mass percentage of 70-75%, the disinfection eggshell with the mass percentage of 1%, and the snail concentrate feed with the mass percentage of 24-29% as the feeding food; (2) placing the bradybaena similaris in the sandy soil of the feeding box, and setting an insect-proof net on the open feeding box; (3) placing the feeding box in a feeding room with the temperature of 25-27℃ and the relative humidity of 70%-80%, using the daylight lamp to provide light, and the day and night alternating cycle is 12h light and 12h darkness; feeding at 8am every day, feeding the food every 24 hours, observing and recording the feeding condition of the bradybaena similaris and cleaning the food residues after 24 hours of feeding, replacing the sandy soil every 7d, adjusting the number of the bradybaena similaris and the amount of food in each feeding box, and monitoring the relative humidity and soil humidity and supplementing water in time; (4) after the bradybaena similaris develops to the brood stage, placing the bradybaena similaris of the brood stage into a new feeding box, laying 8-12cm disinfection sandy soil, then laying 3-5cm disinfection soil, and finally laying a layer of moist moss, placing the bradybaena similaris of the brood stage in the feeding box until the bradybaena similaris lays eggs; (5) carefully taking the bradybaena similaris eggs from the feeding box, selecting qualified bradybaena similaris eggs, then placing the eggs into a new feeding box with disinfection sandy soil, covering the bradybaena similaris eggs with 0.8-1.2cm thick sandy soil, wetting the soil with spray dechlorinated water, the soil surface humidity is 25%-35%, placing the eggs in 28℃, using the daylight lamp to provide light, and the day and night alternating cycle is 9h light and 15h darkness, monitoring the soil surface humidity and supplementing water in time, after the eggs hatch, transferring the bradybaena similaris of the juvenile stage into a new feeding box and continuing to cultivate according to the methods of steps (2)-(4); When the area of the bottom of the feeding box is 0.22-0.25 m 2 , the height is 15-20 cm, 800 snails of the young snail stage of the snail are placed in each feeding box, and the amount of food in each feeding box is 16.8-24.0 g / day; 750-770 snails of the one-month-old snail are placed in each feeding box, and the amount of food in each feeding box is 22.8-26.7 g / day, 690-720 snails of the two-month-old snail are placed in each feeding box, and the amount of food in each feeding box is 24.3-28.7 g / day; 590-620 snails of the three-month-old snail are placed in each feeding box, and the amount of food in each feeding box is 31.3-36.1 g / day; 560-580 snails of the four-month-old snail are placed in each feeding box, and the amount of food in each feeding box is 34.8-37.7 g / day; 460-500 snails of the five-month-old snail are placed in each feeding box, and the amount of food in each feeding box is 33.6-36.7 g / day; 380-420 snails of the breeding snail stage are placed in each feeding box, and the amount of food in each feeding box is 32.3-34.1 g / day; The feeding box is arranged on a layered breeding rack, the breeding rack is arranged with a water pipeline, and a spray head is arranged above each feeding box and communicates with the water pipeline for soil moisture retention.

2. The indoor artificial rearing method of Bradybaena similaris as claimed in claim 1, wherein: The insect-proof net is 20-25 mesh.

3. The indoor artificial rearing method of Bradybaena similaris as claimed in claim 1, wherein: The non-polluted sandy soil is a sandy soil without planting background, and the disinfection is through sunlight exposure for 3-5 days.

4. The indoor artificial rearing method of Bradybaena similaris as claimed in claim 1, wherein: The sandy soil in the feeding box is replaced every 7d, and the soil humidity is adjusted.

5. The indoor artificial rearing method of Bradybaena similaris as claimed in claim 1, wherein: The selection of qualified bradybaena similaris eggs is to spray the surface of the bradybaena similaris eggs with alcohol with a mass concentration of 75% for disinfection, and then rinse with sterile water, then carefully place the eggs in a sterile culture dish with sterilized wet filter paper at the bottom, and select the oval, white, complete mucous membrane protective layer, and green bean size bradybaena similaris eggs with sterile tweezers.

6. The indoor artificial rearing method of Bradybaena similaris as claimed in claim 1, wherein: The cruciferous plants are one or several of cabbage, cauliflower, radish, mustard, and cabbage, and the cucurbitaceae plants are one or several of silk gourd, cucumber, pumpkin, bitter gourd, and winter melon.

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