Semi-artificial feed and artificial feeding method for elephantotracheilus porous

Abstract

The invention belongs to the technical field of insect feeding, and particularly relates to semi-artificial feed and an artificial feeding method for multihole transverse gallosoma elegans. The semi-artificial feed for the rhynchophorus multifortunei, provided by the invention, is prepared from fraxinus chinensis phloem powder, fraxinus chinensis xylem sapwood powder, soybean meal, cane sugar, agar, cholesterol, Webster salt, potassium sorbate, methyl p-hydroxybenzoate, compound vitamin B and distilled water according to a specific weight part ratio. The artificial feeding method comprises the whole processes of adult collection, egg laying induction, egg incubation, single larva feeding, pupa management and adult polyculture. The feed disclosed by the invention is comprehensive and balanced in nutrition, can fully meet the requirement of the whole life history of the multihole transverse gallosopterus, remarkably improves the survival rate of larvae and the eclosion rate of adults, realizes large-scale standardized feeding, and provides a stable insect source for research on pest control.

Description

Technical Field

[0001] This invention belongs to the field of insect breeding technology, specifically relating to a semi-artificial feed for the porous weevil and its artificial breeding method. Background Technology

[0002] Porous transverse sulcus Pimelocerusperforatus Formerly known as the large-grained transverse groove elephant Dysceruscribripennis Matsumura et Kōno. The porous weevil is a devastating borer with a wide host range, primarily damaging many important economic forest trees and ornamental plants, including olive, ash, privet, Japanese privet, lilac, and osmanthus. The pest's damage is extremely destructive: adult females lay eggs at the base of the trunk, and the larvae, after hatching, bore into the base of the trunk, causing the phloem at the base of the trunk to slough off, resulting in a "thousand-hole" appearance, ultimately weakening the tree or even causing its death.

[0003] The life cycle of the porous weevil is extremely complex, with significant generational overlap. Its overwintering stages are diverse: adults of different years overwinter in the soil layer beneath dead leaves and weeds around the base of the trunk, and larvae of various instars hatched in the same year overwinter in the phloem or xylem tunnels at the trunk base and root collar. Overwintering adults emerge as early as late March, initially congregating at the trunk base for a period before climbing onto the branches and leaves of the canopy to feed on the bark for nutrients. They then climb back to the trunk base to mate and lay eggs, repeating this cycle of climbing to replenish nutrients and descending to mate and lay eggs. The peak emergence period for adults is from mid to late April, with the entire emergence period ending in late May.

[0004] In the early stages of damage, adult beetles first gather and lie dormant at the base of the ash tree trunk, then climb to the canopy to feed on branches and leaves to replenish their nutrients. After replenishing their nutrients, they descend the tree and lay their eggs at the base of the trunk or burrow into the ground, specifically at the root collar. Young larvae damage the phloem beneath the old, rough bark, leaving brown frass and debris at the boreholes or bark crevices, appearing rust-brown and moist. Middle-aged larvae bore into the sapwood and wood of the xylem; at this stage, frass no longer exits but instead fills the tunnels and envelops the larvae. Mature larvae construct a pupal chamber around their bodies in the lower half of the tunnel, using dry sawdust to surround their bodies. For trees with a large diameter at breast height (DBH), larvae can bore into the trunk at a height of 1.5–2.0 meters. A yellow, viscous liquid with an unpleasant odor often flows from the boreholes down the trunk. Once the larvae have bored around the trunk once, the tree will die.

[0005] Currently, research on the weevil *Strombus polyporeus* mainly focuses on investigating its harmful characteristics. However, artificial rearing techniques can provide a deeper understanding of the pest's biological and ecological characteristics, offering crucial support for the development of integrated pest management technologies and providing standardized experimental materials for related bioassays. Therefore, there is an urgent need to establish a technical method that can stably rear all developmental stages and obtain a large number of standardized test insects with uniform development to meet the needs of integrated pest management research and behavioral science research on *Strombus polyporeus*.

[0006] In existing technologies, the rearing of *Strombus porphyria* relies heavily on the rhizomes or branches of natural host plants. However, this method has several drawbacks: firstly, the availability of natural host materials is limited by season and region, making year-round continuous rearing impossible; secondly, the nutritional composition of natural hosts is unstable, leading to inconsistent insect development, low survival and emergence rates, and difficulty in meeting the needs of large-scale, standardized experiments. Furthermore, some attempted artificial feed formulations suffer from nutritional imbalances, difficulty in obtaining raw materials, complex formulation, or excessively high costs, failing to achieve efficient rearing of *Strombus porphyria* throughout its entire life cycle. Summary of the Invention

[0007] Based on the above technical problems, the purpose of this invention is to provide a semi-artificial feed for porous transverse groove elephants and a method for artificially raising them.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: This invention provides a semi-artificial feed for the porous transverse groove weevil. The semi-artificial feed comprises the following components in parts by weight: 10-15 parts of ash phloem powder, 10-15 parts of ash sapwood powder, 9-11 parts of soybean powder, 3-5 parts of sucrose, 5-7 parts of agar, 0.05-0.1 parts of cholesterol, 0.2-0.4 parts of Wechsler salt, 0.2-0.4 parts of methyl sorbate, 0.2-0.4 parts of methylparaben, 0.5-1.5 parts of compound vitamin B, and 100-200 parts of distilled water.

[0009] The core concept of this invention lies in: targeting the feeding characteristics of the porous weevil, a borer, and mimicking the nutritional structure of its natural host, the ash tree. A specific ratio of ash phloem powder and xylem sapwood powder is used as the main component, supplemented with soybean flour, sucrose, agar, cholesterol, Widmanstätten salt, potassium sorbate, methylparaben, B-complex vitamins, and preservatives. This mixture is then prepared through steam sterilization, mixing, and agar solidification to create a nutritionally balanced and easily digestible semi-solid feed. Based on this, a standardized rearing method is established covering the entire process from adult collection, oviposition induction, egg hatching, single-larval rearing, pupal management, and mixed-species breeding, clearly defining the key environmental parameters for each stage. This invention solves the problems of unstable nutrition, seasonal limitations and low survival rate caused by dependence on natural hosts through synergistic innovation of feed and feeding methods. It significantly improves the larval survival rate (up to 90%), shortens the development period, and increases the adult emergence rate, enabling large-scale and standardized year-round feeding of the weevil, and providing a stable source of insects for biological research and integrated pest management technology development of this pest.

[0010] Furthermore, the fineness of the ash tree phloem powder and ash tree sapwood powder is 70-90 mesh, and the fineness of the potato starch is 90-110 mesh.

[0011] This invention also provides a method for preparing the semi-artificial feed, the method comprising the following steps: S1. Mix the phloem powder, sapwood powder and soybean powder evenly, sterilize with steam and cool to room temperature to obtain sterilized material. S2. Dissolve sucrose, cholesterol, Widmanstätten salt, potassium sorbate, methyl paraben, and vitamin B complex in water to obtain a mixture. Add agar to boiling water until the agar dissolves to form an agar solution. S3. Add the sterilized materials and mixture to the agar solution and mix well. Dispense into feeding boxes and allow to cool naturally to room temperature to solidify, thus obtaining semi-artificial feed.

[0012] Furthermore, the sterilization conditions are sterilization at 110℃~130℃ for 20~30 minutes.

[0013] The present invention also provides an artificial rearing method for the porous weevil, comprising the step of placing the porous weevil larvae on the semi-artificial feed for rearing. The method is used to shorten the larval development cycle and improve the larval survival rate and pupation rate.

[0014] Furthermore, the steps for obtaining the porous transverse groove weevil larvae are as follows: (1) Collect adult weevils of the porous transverse groove and place them in a rearing box. The rearing density is 1-2 individuals / cm². Place fresh ash tree branches in the rearing box and change them 1-2 times a day. Collect the eggs in the rearing box every 20-30 hours. (2) Place the eggs in a completely dark constant temperature incubator at 26℃~28℃ and 85%~95% relative humidity to hatch them, and collect the hatched larvae.

[0015] Furthermore, the adult insect rearing conditions are 20℃~30℃ and relative humidity 70%~80%.

[0016] Furthermore, the rearing conditions for the larvae are as follows: the hatched larvae are placed in a rearing box containing the aforementioned semi-artificial feed and kept under constant temperature conditions of 26℃~28℃, relative humidity of 60%~70%, and light / dark cycle of 12h / 12h. Fresh feed is replaced 1~2 times every 9~11 days until pupation.

[0017] Furthermore, after the pupae emerge as adults, they are raised in a rearing box with males and females in a 1:1 ratio. Fresh ash tree branches are placed in the rearing box and replaced 1-2 times a day. Eggs are collected from the rearing box every 20-30 hours, and the rearing process is repeated until pupation.

[0018] The present invention has the following beneficial effects: The semi-artificial feed provided by this invention is nutritionally comprehensive and balanced, meeting the nutritional needs of the weevil from egg to adult throughout its entire life cycle. The raw materials are widely available, inexpensive, and easy to prepare. The accompanying artificial rearing method is standardized and highly controllable, enabling large-scale and standardized rearing of the weevil and providing a stable and high-quality source of experimental insects for related research on this pest. Attached Figure Description

[0019] Figure 1 The diagram shows the rearing of the porous weevil, where A is the rearing diagram of adults, B is the hatching diagram of eggs, and C is the rearing diagram of larvae. Detailed Implementation

[0020] The present invention will now be described in detail with reference to specific embodiments, but these should not be construed as limiting the invention. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following embodiments can be obtained commercially unless otherwise specified.

[0021] In the following embodiments and comparative examples: Ash phloem powder: Peel the phloem of the ash tree with a machete, dry it indoors, and then cut the phloem into small pieces and grind it into powder using a grinder.

[0022] Ash wood sapwood powder: Use a plane to obtain the sapwood of ash tree trunks after removing the phloem, place it indoors to dry, and then cut the ash tree trunk sapwood into small pieces and grind it into powder using a grinder.

[0023] Soybean flour was purchased from Guizhou Century Brilliant Industry Co., Ltd., sucrose from Wuxi Yatai United Chemical Co., Ltd., agar from Beijing Aoboxing Biotechnology Co., Ltd., cholesterol from Shanghai Fantai Biotechnology Co., Ltd., Wechsler salt from Wuhan Terhui Biotechnology Co., Ltd., methyl sorbate from Shanghai Yuanye Biotechnology Co., Ltd., methylparaben from Shanghai Yuanye Biotechnology Co., Ltd., and compound vitamin B (National Drug Approval Number H31021754) from Shanghai Xinhuanghe Pharmaceutical Co., Ltd.

[0024] Example 1 I. Formulation of semi-artificial feed for porous transverse groove elephants.

[0025] 1. Feed composition: Weigh the following components by weight: 12 parts of ash phloem powder (80 mesh), 12 parts of ash sapwood powder (80 mesh), 10 parts of soybean powder (100 mesh), 4 parts of sucrose, 6 parts of agar, 0.09 parts of cholesterol, 0.3 parts of Wechsler salt, 0.3 parts of methyl sorbate, 0.3 parts of methyl paraben, 0.8 parts of compound vitamin B, and 150 parts of distilled water.

[0026] 2. Preparation method: (1) Raw material pretreatment: Mix the phloem powder, sapwood powder and soybean powder evenly, put them into a high-pressure steam sterilizer, sterilize at 121°C for 25 minutes, take them out and cool to room temperature to obtain sterilized material.

[0027] (2) Preparation of mixture: Add sucrose, cholesterol, Wechsler salt, potassium sorbate, methylparaben and compound vitamin B to 30 ml of distilled water (20% of the total weight of distilled water), stir with a magnetic stirrer for 10 minutes until all components are completely dissolved to obtain the mixture.

[0028] (3) Preparation of agar solution: Add the remaining 120ml of distilled water to a beaker, place it on an induction cooker and boil it. Then add agar in small amounts several times while stirring with a glass rod until the agar is completely melted and a uniform agar solution is formed.

[0029] (4) Feed preparation: Sterilize the materials and mixtures and slowly add them to the agar solution while stirring to ensure that the components are mixed evenly and form a uniform feed paste.

[0030] (5) Packaging and storage: While the feed paste is still hot, it is packaged into 50ml plastic feeding boxes sterilized with 75% ethanol by volume. Each feeding box contains 25g of feed. After naturally cooling to room temperature and solidifying, the porous transverse groove elephant semi-artificial feed is obtained. The box is covered and sealed in a refrigerator at 4℃ for later use.

[0031] II. Artificial feeding method using porous transverse groove elephant semi-artificial feed.

[0032] 1. Adult Collection and Oviposition: Adult *Echinochloa multifilamentosa* were collected from infested ash forests in Tai'an, Shandong Province. Robust individuals with strong activity were selected and acclimatized for 3 days in a constant-temperature incubator at 25℃ and 75% relative humidity. Fresh ash branches were replaced daily, and weak, sick, or dead individuals were removed. The selected adults were then placed in transparent plastic rearing boxes (30cm×20cm×10cm) lined with filter paper at a density of 1 individual / cm². Fresh ash branches were placed inside the boxes and replaced daily. Eggs were collected from the surface of the filter paper at the bottom of the rearing box every 24 hours.

[0033] 2. Egg hatching: Gently transfer the collected eggs to a petri dish (9cm in diameter) lined with two layers of moist kitchen paper. The kitchen paper should be kept moist but not dripping. Place the petri dish in a constant temperature incubator at 27℃, 90% relative humidity, and complete darkness. Observe once a day, record the hatching status of the eggs, and collect the newly hatched larvae.

[0034] 3. Larval rearing: Newly hatched larvae are transferred one by one with a soft brush to a 50ml plastic rearing box containing semi-artificial feed for the porous weevils. One larva is reared in each box. The lid of the rearing box is punched with 10 ventilation holes with a diameter of 1mm. The boxes are placed in a constant temperature incubator with a temperature of 27℃, relative humidity of 65%, and a light-dark cycle of 12L:12D. Fresh feed is changed every 10 days. At the same time, feces and leftover feed in the rearing box are cleaned with sterile cotton swabs. The survival rate, molting status, and developmental stage of the larvae are recorded.

[0035] 4. Pupa emergence: After the larvae stop feeding and pupate in the feed, keep the rearing environment parameters unchanged and observe the emergence of the pupae daily. Once the pupae have emerged as adults, promptly transfer the adults to a new transparent plastic rearing box using a soft brush.

[0036] 5. Adult rearing and breeding: Newly emerged adults are reared in a 1:1 female-to-male ratio in transparent plastic rearing boxes measuring 30cm × 20cm × 10cm. Fresh ash tree branches are placed inside the boxes and replaced daily. Eggs are collected periodically. The above steps are repeated to achieve continuous rearing of the *Periphera polyporeis*. Hatching steps are as follows: Figure 1 As shown.

[0037] Example 2 I. Formulation of semi-artificial feed for porous transverse groove elephants.

[0038] 1. Weigh each component according to the following parts by weight: 10 parts of ash phloem powder (70 mesh), 15 parts of ash sapwood powder (90 mesh), 8 parts of soybean powder (100 mesh), 3 parts of sucrose, 4 parts of agar, 0.08 parts of cholesterol, 0.2 parts of Wechsler salt, 0.2 parts of potassium sorbate, 0.2 parts of methylparaben, 0.5 parts of compound vitamin B, and 100 parts of distilled water.

[0039] 2. Feed preparation: (1) Raw material pretreatment: Mix the phloem powder, sapwood powder and soybean powder evenly, sterilize with high pressure steam at 121℃ for 20 minutes, and obtain sterilized material after cooling.

[0040] (2) Preparation of mixture: Add sucrose, cholesterol, Wechsler salt, potassium sorbate, methylparaben and compound vitamin B to 10 ml of distilled water (10% of the total weight of distilled water) and stir until completely dissolved to obtain mixture.

[0041] (3) Preparation of agar solution: Boil the remaining 90ml of distilled water, add agar and stir until completely dissolved to obtain agar solution.

[0042] (4) Feed preparation: gradually add the sterilized materials and mixtures into the agar solution and stir evenly.

[0043] (5) Repackaging and storage: Repackage the feed into 50ml plastic feeding boxes sterilized with 75% ethanol by volume. Each feeding box contains 20g of feed. After cooling and solidification, store in a sealed refrigerator at 4℃ for later use.

[0044] II. Artificial feeding method using porous transverse groove elephant semi-artificial feed.

[0045] 1. Adult collection and oviposition: Adult *Porous transverse groove weevils* were collected from damaged olive groves in Longnan, Gansu Province. After acclimatization for 2 days, healthy individuals were selected and placed in transparent rearing boxes at a density of 1.5 individuals / cm². Fresh ash branches were replaced daily, and eggs were collected regularly.

[0046] 2. Egg hatching: Transfer the eggs to a petri dish lined with three layers of moist kitchen paper and place it in a completely dark environment at a temperature of 26℃ and a relative humidity of 85%. Observe and collect the newly hatched larvae daily. 3. Larval rearing: Newly hatched larvae are reared individually in 50ml rearing boxes containing the above-mentioned semi-artificial feed. The rearing environment is maintained at a temperature of 25℃, a relative humidity of 60%, and a light / dark cycle of 12h / 12h. The feed is changed every 10 days, and feces and uneaten feed are cleaned up.

[0047] 4. Pupae emergence and adult rearing: Keep the rearing environment parameters unchanged. After the pupae emerge, mix the males and females in a 1:1 ratio and provide fresh ash tree branches to achieve continuous rearing.

[0048] Example 3 I. Preparation of Semi-Artificial Feed for Porous Transverse Grooved Elephants 1. Weigh the following components according to the following weight proportions: 15 parts of ash phloem powder (90 mesh), 10 parts of ash sapwood powder (70 mesh), 12 parts of soybean powder (100 mesh), 5 parts of sucrose, 8 parts of agar, 0.10 parts of cholesterol, 0.5 parts of Wechsler salt, 0.4 parts of potassium sorbate, 0.4 parts of methylparaben, 1.0 part of compound vitamin B, and 200 parts of distilled water.

[0049] 2. Preparation method (1) Raw material pretreatment: Mix the phloem powder, sapwood powder and soybean powder evenly, sterilize with high pressure steam at 121℃ for 30 minutes, and obtain sterilized material after cooling.

[0050] (2) Preparation of mixture: Add sucrose, cholesterol, Wechsler salt, potassium sorbate, methylparaben and compound vitamin B to 40 ml of distilled water (20% of the total weight of distilled water) and stir until completely dissolved to obtain mixture.

[0051] (3) Preparation of agar solution: Boil the remaining 160ml of distilled water, add agar and stir until completely dissolved to obtain agar solution.

[0052] (4) Feed preparation: gradually add the sterilized materials and mixtures into the agar solution and stir evenly.

[0053] (5) Repackaging and storage: Repackage the feed into 100ml plastic feeding boxes sterilized with 75% ethanol by volume. Each feeding box contains 30g of feed. After cooling and solidification, store in a sealed refrigerator at 4℃ for later use.

[0054] II. Artificial feeding method using porous transverse groove elephant semi-artificial feed.

[0055] 1. Adult collection and oviposition: Adult *Echinochloa pulmonata* were collected from damaged ash trees in the afforestation area of ​​the Beijing Plain. After acclimatization for 3 days, healthy individuals were selected and placed in transparent rearing boxes at a density of 2 individuals / cm². Fresh ash tree branches were replaced daily, and eggs were collected regularly.

[0056] 2. Egg hatching: Transfer the eggs to a petri dish lined with two layers of moist kitchen paper and place it in a completely dark environment at a temperature of 27℃ and a relative humidity of 95%. Observe and collect the newly hatched larvae daily.

[0057] 3. Larval rearing: Newly hatched larvae are reared individually in 100ml rearing boxes containing the above-mentioned semi-artificial feed. The rearing environment is maintained at a temperature of 26℃, a relative humidity of 70%, and a light-dark cycle of 12L:12D. The feed is changed every 10 days, and feces and leftover feed are cleaned up.

[0058] 4. Pupae and adult rearing: Keep the rearing environment parameters unchanged. After the pupae emerge, mix the males and females in a 1:1 ratio and provide fresh ash tree branches to achieve continuous rearing.

[0059] Comparative Example 1 I. Preparation of Semi-Artificial Feed for Porous Transverse Grooved Elephants 1. Semi-artificial feed formula for porous transverse grooved elephants: 24 parts of ash phloem powder (80 mesh), 10 parts of soybean powder (100 mesh), 4 parts of sucrose, 6 parts of agar, 0.09 parts of cholesterol, 0.3 parts of Wechsler salt, 0.3 parts of potassium sorbate, 0.3 parts of methylparaben, 0.8 parts of compound vitamin B, and 150 parts of distilled water.

[0060] 2. The preparation method and artificial breeding method are the same as in Example 1.

[0061] Comparative Example 2 1. Semi-artificial feed formula for porous transverse grooved elephants: 24 parts ash wood sapwood powder (80 mesh), 10 parts soybean powder (100 mesh), 4 parts sucrose, 6 parts agar, 0.09 parts cholesterol, 0.3 parts Wechsler salt, 0.3 parts potassium sorbate, 0.3 parts methylparaben, 0.8 parts compound vitamin B, and 150 parts distilled water.

[0062] 2. The preparation method and artificial breeding method are the same as in Example 1.

[0063] Comparative Example 3 Fresh ash tree rhizomes were used as feeding material, and the porous transverse groove elephants were raised according to the artificial rearing method of Example 1 (except that the feed was replaced with fresh ash tree rhizomes, and all other conditions were the same).

[0064] Eggs and larvae from the same batch of adults of the porous weevil collected in the wild, along with their hatched larvae, were used as the insect source. They were reared using the semi-artificial feeds of Examples 1-3 and Comparative Examples 1-2, and fresh ash tree rhizomes of Comparative Example 3. Each group was reared with 30 eggs and 30 larvae, repeated 5 times, for a total of 750 eggs and 750 larvae. Observations were made daily to record the egg hatching rate; larval survival rate and developmental period were recorded every 7 days; pupation rate was recorded after larval pupation; and the weight of male and female adults was measured after emergence.

[0065] Table 1: Comparison of the effects of semi-artificial diet on the porous transverse grooved weevils in each group Note: / indicates no data.

[0066] As shown in Table 1, the semi-artificial feed used in Examples 1-3 of this invention is significantly better than that used in Comparative Examples 1-3. The larval survival rate of Examples 1-3 is above 83%, with Example 1 reaching 90%. The larval development period is within 57 days, significantly shorter than that of Comparative Examples 1-3. The weight of mature larvae, female adults, and male adults are all higher than those of Comparative Examples 1-2, and the pupation rate is also at a high level (80.00%~92.59%).

[0067] Comparative Example 1 used only phloem powder of ash tree, and Comparative Example 2 used only xylem sapwood powder of ash tree. The larval survival rate, development period, larval and adult weight of the two were not as good as those of the embodiment of the present invention. This shows that the reasonable combination of phloem powder and xylem sapwood powder of ash tree can significantly improve the nutritional suitability of the feed. Using only one of the raw materials cannot meet the optimal growth requirements of the porous weevil.

[0068] Comparative Example 3, which uses the rootstock of the natural host tree ash, showed a larval survival rate of only 3.33%, significantly lower than that of the embodiment of the present invention. Furthermore, the larval development period was the longest (70 days), and the weight of mature larvae and adults was the lowest. Although the pupation rate reached 100%, the overall rearing effect was extremely poor, making it impossible to achieve large-scale, standardized rearing. This further demonstrates the superiority of the semi-artificial feed of the present invention.

[0069] In summary, the semi-artificial feed formula and feeding method of Example 1 of this invention have the best effect, with the highest larval survival rate (90.00%), the shortest development period (50.25 days), the highest pupation rate (92.59%), and the highest larval and adult weights. It can meet the needs of large-scale and standardized breeding of the weevil to the greatest extent.

[0070] It should be noted that when numerical ranges are mentioned in the claims of this invention, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, the present invention describes preferred embodiments.

[0071] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the invention.

[0072] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A semi-artificial feed for porous transverse grooved weevils, characterized in that, The semi-artificial feed, by weight, is made from the following raw materials: 10-15 parts ash phloem powder, 10-15 parts ash sapwood powder, 9-11 parts soybean flour, 3-5 parts sucrose, 5-7 parts agar, 0.05-0.1 parts cholesterol, 0.2-0.4 parts Wiener salt, 0.2-0.4 parts methyl sorbate, 0.2-0.4 parts methylparaben, 0.5-1.5 parts compound vitamin B, and 100-200 parts distilled water.

2. The semi-artificial feed according to claim 1, characterized in that, The fineness of the phloem powder and sapwood powder of ash tree is 70-90 mesh, and the fineness of the potato starch is 90-110 mesh.

3. The method for preparing the semi-artificial feed according to claim 1, characterized in that, Includes the following steps: S1. Mix the phloem powder, sapwood powder and soybean powder evenly, sterilize with steam and cool to room temperature to obtain sterilized material. S2. Dissolve sucrose, cholesterol, Widmanstätten salt, potassium sorbate, methyl paraben, and vitamin B complex in water to obtain a mixture. Add agar to boiling water until the agar dissolves to form an agar solution. S3. Add the sterilized material and mixture to the agar solution and mix well. Allow it to cool naturally to room temperature to solidify and obtain semi-artificial feed.

4. The preparation method according to claim 3, characterized in that, The sterilization conditions are 110℃~130℃ for 20~30 minutes.

5. A method for artificially raising the porous transverse groove elephant, characterized in that, The method includes the step of feeding porous transverse groove weevil larvae on the semi-artificial feed described in any one of claims 1 to 2, wherein the method is used to shorten the larval development cycle and improve the larval survival rate and pupation rate.

6. The artificial breeding method according to claim 5, characterized in that, The steps for obtaining the porous transverse groove weevil larvae are as follows: (1) Collect adult weevils of the porous transverse groove and place them in a rearing box. The rearing density is 1-2 individuals / cm². Place fresh ash tree branches in the rearing box and change them 1-2 times a day. Collect the eggs in the rearing box every 20-30 hours. (2) Place the eggs in a completely dark constant temperature incubator at 26℃~28℃ and 85%~95% relative humidity to hatch them, and collect the hatched larvae.

7. The artificial breeding method according to claim 6, characterized in that, The adult insect rearing conditions are 20℃~30℃ and relative humidity 70%~80%.

8. The artificial breeding method according to claim 6, characterized in that, The larvae are raised in a rearing box containing the semi-artificial feed described above, at a constant temperature of 26℃~28℃, relative humidity of 60%~70%, and a light / dark cycle of 12h / 12h. Fresh feed is changed 1~2 times every 9~11 days until pupation.

9. The artificial breeding method according to claim 8, characterized in that, After the pupae emerge as adults, they are raised in a 1:1 male-to-female ratio in a rearing box. Fresh ash tree branches are placed in the rearing box and replaced 1-2 times a day. Eggs are collected from the rearing box every 20-30 hours. This rearing process is repeated until the pupae pupate.

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