A bioreactor for plant tissue

Abstract

The application discloses a kind of biological reaction devices of plant tissue, comprising, culture device, for the culture of plant tissue, the outer wall of the culture device is provided with detection device, for detecting the parameter and / or sampling of culture solution in the culture device;Heat transfer structure, for the temperature control of the culture device, the heat transfer structure has notch, the detection device is arranged on the outer wall of the culture device in the notch.The plant tissue is cultured by the culture device in the application, the temperature in the device is kept stable by heat transfer structure, prevent the influence caused by external temperature to the environment in the device.And detection device is arranged on the outer wall of the culture device in the notch of heat transfer structure, can avoid the damage or heat loss of heat transfer structure caused by detection device passing through heat transfer structure, ensure the integrity of heat transfer structure, make temperature keep stable, plant tissue better growth.

Description

Technical Field

[0001] This invention belongs to the field of plant tissue culture devices, and more specifically, relates to a bioreactor for plant tissues. Background Technology

[0002] With the continuous improvement of living standards, people are paying increasing attention to strengthening their constitution and maintaining health through the consumption of medicinal herbs with nourishing effects. Due to the huge population, the demand for medicinal plants has risen sharply. Furthermore, cultivating medicinal plants using traditional methods requires not only large amounts of land and a long growth cycle, but also a suitable climate. Any unsuitable conditions will limit and reduce the yield of medicinal plants.

[0003] Therefore, researchers have developed a method and cultivation device for large-scale cultivation using isolated plant tissues or cells. By separating plant organs such as roots, stems, and leaves, and then placing them in a nutrient-rich culture medium while providing suitable temperature, light, and other environmental conditions, callus, adventitious buds, and adventitious roots are induced in the plant organs. Finally, these callus, adventitious buds, and adventitious roots are used as seeds for cultivating plants, and then placed in the cultivation device for further cultivation.

[0004] In the large-scale production of plant tissues, temperature control of the culture device is crucial for the growth of plant tissues, especially adventitious roots. Excessive or insufficient temperature, as well as uneven heating, can damage plant tissue growth. In existing technologies, the detection device in the culture device passes through a sealed insulation layer. However, due to occasional high temperatures and large temperature fluctuations, the seal may be damaged, leading to leakage of the heat transfer medium and damage to the detection device. Furthermore, the heat transferred at the sealed point differs from that at other locations, which may affect temperature control within the culture device and result in slow plant tissue growth.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a bioreactor for plant tissue, which has a culture device and a heat transfer structure. The heat transfer structure has a notch, and the detection device is installed on the exposed outer wall of the culture device inside the notch. This can avoid uneven heat transfer caused by the detection device passing through the heat transfer structure and being damaged by sealing. In addition, the heat transfer structure is easier to install.

[0007] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by the present invention is: a bioreactor for plant tissue, comprising,

[0008] A culture device for culturing plant tissues, wherein a detection device is provided on the outer peripheral wall of the culture device for detecting parameters of the culture medium inside the culture device and / or taking samples;

[0009] The heat transfer structure is a jacket fitted on the outer peripheral wall of the culture device for temperature control of the culture device. The lower end of the jacket has a notch that extends downward through the lower edge of the jacket. The detection device is located on the outer peripheral wall of the culture device within the notch.

[0010] Furthermore, the culture device has a first tank and a second tank connected together, the second tank extending downward from the lower end of the first tank, and the inner diameter gradually decreasing from the inner diameter of the first tank;

[0011] The heat transfer structure includes a first annular sleeve and a second annular sleeve that are connected and are respectively sleeved on the outer periphery of the connection end of the first tank and the second tank. The notch extends upward from the lower edge of the second annular sleeve and is provided at least on the second annular sleeve.

[0012] Furthermore, the cultivation device includes a seed tank for cultivating plant tissues, and the heat transfer structure is sleeved on the seed tank. The first annular sleeve and the second annular sleeve of the heat transfer structure are respectively sleeved on the outer periphery of the connecting end of the first tank body and the second tank body of the seed tank. The notch of the heat transfer structure extends upward from the lower edge of the second annular sleeve to the first annular sleeve.

[0013] Furthermore, the first annular sleeve of the seed tank extends from the upper part of the first tank body to the edge where the first tank body and the second tank body connect; the second annular sleeve of the seed tank extends downward from the lower end of the first annular sleeve to slightly exceed the edge where the second tank body and the first tank body connect, and the thickness of the second annular sleeve of the seed tank gradually increases from top to bottom.

[0014] The notch in the heat transfer structure of the seed tank extends upward from the lower edge of the second annular sleeve on the seed tank to more than half the axial extension length of the first annular sleeve.

[0015] Furthermore, the cultivation device also includes a cultivation tank for secondary cultivation of plant tissues cultivated in the seed tank, which is circulated and connected to the seed tank. The heat transfer structure is sleeved on the cultivation tank. The first annular sleeve and the second annular sleeve of the heat transfer structure are respectively sleeved on the outer periphery of the connection end of the first tank body and the second tank body of the cultivation tank. The maximum inner diameter of the first tank body of the cultivation tank is much larger than the maximum inner diameter of the first tank body of the seed tank. The notch of the heat transfer structure extends upward from the lower edge of the second annular sleeve onto the second annular sleeve.

[0016] Furthermore, the first annular sleeve of the culture tank extends from the upper part of the first tank body to the edge where the first tank body and the second tank body connect;

[0017] The second annular sleeve of the culture tank extends downward from the lower end of the first annular sleeve along the second tank body for more than half the axial extension length of the second tank body;

[0018] The notch in the heat transfer structure of the culture tank extends upward from the lower edge of the second annular sleeve on the culture tank to more than half the axial extension length of the second annular sleeve.

[0019] Furthermore, the second tank at the bottom of the seed tank is provided with at least two sets of air intake structures for supplying air into the tank, and the air intake structures are located below the heat transfer structure of the seed tank; the air intake structures of the seed tank are arranged opposite to each other on the peripheral wall of the second tank of the seed tank.

[0020] The second tank at the bottom of the culture tank is provided with at least four sets of air intake structures for supplying air into the tank. The air intake structures are located below the heat transfer structure of the culture tank. The air intake structures of the culture tank are evenly distributed circumferentially on the outer peripheral wall of the second tank of the culture tank.

[0021] Furthermore, the two sets of air intake structures of the seed tank extend into the interior of the second tank body. The portions of the two sets of air intake structures that extend into the tank body have air intake rods that are kept horizontal and have a certain length, which deliver sterile air into the interior of the second tank body in the form of bubbles. One set of air intake structures of the seed tank is located directly below the notch, and the other set of air intake structures is symmetrically arranged with the air intake structure below the notch.

[0022] Furthermore, the four sets of air intake structures of the culture tank extend upwards into the second tank body; the part of the air intake structure extending into the tank has an air intake rod that is kept horizontal and has a certain length, which delivers sterile air into the second tank body of the culture tank in the form of bubbles; the projections of any two opposing sets of air intake rods in the culture tank body are approximately parallel, and the projections of two adjacent sets of air intake rods in the tank body are approximately perpendicular.

[0023] One set of air intake structures of the culture vessel is located directly below the notch, and the air intake structures are circumferentially spaced on the second body of the culture vessel.

[0024] Furthermore, at least two sets of cutting structures are provided on the seed container, and the air intake structure on the seed container is provided at intervals with the cutting structures;

[0025] At least two sets of cutting structures are provided on the culture tank. The installation position of the cutting structure on the culture tank and the line connecting the installation positions of the two adjacent air inlet structures approximately form an isosceles triangle. The installation position of the cutting structure is higher than the air inlet structure in the vertical direction.

[0026] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art.

[0027] (1) The heat transfer structure of this invention has a notch, which avoids the location of the detection device. This avoids the aging, deformation, or failure of the sealing material caused by high temperatures, prevents damage to the sealing structure from high temperatures, reduces the frequency and cost of replacement and maintenance, and can also effectively prevent the leakage of the heat transfer medium, reduce the risk of accidents and disasters, and improve workplace safety. Placing the detection device on the outer wall of the cultivation device within the notch of the heat transfer structure makes it easier to maintain and operate the device. Compared to directly passing through the heat transfer structure, only the notch needs to be operated and adjusted, avoiding the process of disassembling and reinstalling the heat transfer structure, reducing workload and time consumption. It can also avoid the difference in heat transfer between the sealing point and other locations, which could lead to inaccurate temperature control inside the tank and affect the growth rate of plant tissues.

[0028] (2) The present invention achieves accurate temperature control within the culture device by installing a heat transfer structure on the culture device, providing a suitable growth environment for plant tissues, which is beneficial to the nutrient absorption and growth and development of plant tissues and accelerates the growth rate of plant tissues.

[0029] The specific embodiments of the present invention will now be described in further detail with reference to the accompanying drawings. Attached Figure Description

[0030] The accompanying drawings, as part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention, but do not constitute an undue limitation of the invention. Obviously, the drawings described below are merely some embodiments, and those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:

[0031] Figure 1 This is a schematic diagram of the bioreactor for plant tissues according to the present invention;

[0032] Figure 2 This is a schematic diagram of the heat transfer structure of the present invention;

[0033] Figure 3 This is a schematic diagram of the water tank piping of the present invention;

[0034] Figure 4 This is a schematic diagram of the overall external structure of the present invention;

[0035] Figure 5 This is a schematic diagram of the cutting structure and air intake structure of the culture tank;

[0036] Figure 6 This is a schematic diagram of the seed tank cutting structure and air intake structure;

[0037] Figure 7 This is a schematic diagram of the air intake structure of the present invention from one angle;

[0038] Figure 8 This is a schematic diagram of the cutting structure of the present invention;

[0039] Figure 9 This is a schematic diagram of the shearing section of the cutting structure of the present invention;

[0040] Figure 10 This is a schematic diagram of the temperature control system of the present invention.

[0041] In the diagram: 11. Detection device; 12. First tank; 13. Second tank; 14. Seed tank; 141. First discharge port; 142. First receiving port; 15. Culture tank; 151. Second discharge port; 152. Second receiving port; 16. Conveying pipeline; 161. Seed transfer pipe; 162. Seed return pipe; 2. Heat transfer structure; 21. Notch; 22. First annular sleeve; 23. Second annular sleeve; 24. Insulation layer; 241. First opening; 242. Second opening; 3. Air intake structure; 31. Air guide bracket; 311. First straight section; 312. Second straight section; 313. Third straight section; 32. Fixing ring; 33. Fixing plate; 34. 4. Air intake rod; 4. Cutting structure; 41. Spacer; 42. Shearing part; 421. Pin; 422. Blade assembly; 423. First blade; 424. Second blade; 425. Rotating end; 426. Blade end; 427. Closed side; 428. Unfolding side; 43. Transmission part; 44. Power part; 45. Flow guide; 451. Inlet; 453. Outlet; 5. Water tank; 51. Water outlet pipe; 52. Water inlet pipe; B100. Steam supply main pipe; C200. Chilled water supply main pipe; E200. Hot water supply main pipe; A3. Temperature-regulating water tank; A301. Water chamber; A24. Liquid inlet pipe; A25. Liquid outlet pipe.

[0042] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the invention in any way, but rather to illustrate the concept of the invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0043] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0044] In the description of this invention, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0045] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0046] Temperature is one of the key factors affecting the growth rate of plant tissues, especially adventitious roots. Suitable temperatures provide the optimal environmental conditions for plant tissue growth; however, excessively high or low temperatures can lead to abnormal or stagnant growth. Therefore, accurate temperature control is crucial for plant tissue growth to ensure growth rate and improve plant tissue yield and quality.

[0047] like Figures 1 to 3 As shown, this invention provides a bioreactor for cultivating ginseng adventitious roots, comprising a culture device and a heat transfer structure 2. The culture device contains a culture medium to promote the growth of ginseng adventitious roots. A detection device 11 is installed on the peripheral wall of the culture device, comprising multiple detection units and sampling units for detecting parameters of the culture medium and / or sampling. The heat transfer structure 2 is a jacket fitted onto the peripheral wall of the culture device for temperature control. The heat transfer structure 2 has a notch 21, and the detection device 11 is located on the outer peripheral wall of the culture device within the notch 21.

[0048] The detection device 11 is installed on the outer peripheral wall of the culture device through the notch 21 from the outside and extends into the interior of the culture device.

[0049] This invention controls the temperature within the cultivation device by incorporating a heat transfer structure 2. The heat transfer structure 2 provides thermal insulation, which helps stabilize the temperature within the cultivation device, effectively preventing external temperatures from having an unnecessary impact on the cultivation process, maintaining temperature stability, providing a suitable growth environment temperature for ginseng adventitious roots, increasing yield and production efficiency, accelerating the cultivation process, and shortening the production cycle.

[0050] In this invention, the heat transfer structure 2 has a notch 21, through which the outer wall of the culture device is directly exposed. The detection device 11 is directly installed on the exposed outer wall of the culture device within the notch 21, facilitating the installation, operation, and adjustment of the detection device 11. Compared to directly passing through the heat transfer structure 2, operation and adjustment only need to be performed at the notch 21, avoiding the process of disassembling and reinstalling the heat transfer structure 2, thus reducing workload and time consumption. Since there is no need to seal between the detection device 11 and the heat transfer structure 2, it also avoids the aging, deformation, or failure of the sealing material that is easily caused by high temperatures, preventing damage to the sealing structure from high temperatures, reducing the frequency and cost of replacement and maintenance, and effectively preventing leakage of the heat transfer medium, reducing the risk of accidents and disasters, and improving workplace safety. The lower edge of the heat transfer structure 2 has a through notch 21, which allows for convenient installation of the heat transfer structure 2 onto the culture device.

[0051] The notch 21 extends through both the inner and outer sides of the heat transfer structure 2. The outer wall of the culture device is exposed through the notch 21 and is in direct contact with the external atmospheric environment. The heat transfer structure 2 with the notch 21 has an internal cavity. The heat transfer structure 2 is fitted onto the outer peripheral wall of the culture device.

[0052] The detection device 11 is at a certain distance from the edge of the notch 21. The heat transfer structure 2 has a certain thickness, and the wall thickness of the heat transfer structure 2 extends through the inside and outside of the notch 21. The heat transfer structure 2 has a sealing wall that seals the inner peripheral wall of the notch 21, forming the cavity.

[0053] Preferably, the heat transfer structure 2 is fitted in the middle of the culture device.

[0054] The culture device is equipped with feeding and discharging devices at its upper and lower ends for adding nutrients or extracting the culture medium. The heat transfer structure 2 is fitted into the middle of the culture device, with a notch 21 located at the lower end of the jacket. This avoids the locations of the feeding and discharging devices on the culture device, preventing complex installation of the heat transfer structure 2 and avoiding resource waste. The jacket can evenly distribute heat around the outer perimeter of the culture device, ensuring uniform heating of the culture medium or sample.

[0055] The culture device has a first tank 12 and a second tank 13 connected together. The second tank 13 extends downward from the lower end of the first tank 12, and its inner diameter gradually decreases from that of the first tank 12. The heat transfer structure 2 includes a first annular sleeve 22 and a second annular sleeve 23 connected together, which are respectively fitted around the outer periphery of the connecting end of the first tank 12 and the second tank 13. The notch 21 extends upward from the lower edge of the second annular sleeve 23.

[0056] The heat transfer structure 2 is installed on the outer periphery of the connection end between the first tank 12 and the second tank 13 of the culture device, and is used to heat the middle part of the culture device. Heat is transferred to the culture medium inside the culture device through the outer wall of the culture device.

[0057] Preferably, in this invention, the upper part of the culture device is a first tank 12, which is cylindrical and divided into a first part and a second part. The first part is a dome-shaped structure with a apex at the top, and its inner diameter gradually increases downward from the apex. The second part is a cylindrical structure with openings at both ends, connected to the first part at the top, extending downwards for a certain distance, and connected to the second tank 13 at the bottom. The second tank 13 is an inverted cone shape, with its upper end connected to the second part of the first tank 12. The bottom surface of the first tank 12 and the top surface of the second tank 13 are open surfaces, and the interiors of the first tank 12 and the second tank 13 are interconnected.

[0058] The notch 21 can extend upward from the lower edge of the second annular sleeve 23 onto the second annular sleeve 23, or it can extend to the first annular sleeve 22. The extension of the notch 21 ensures that sufficient installation space is reserved for the installation of the detection unit and sampling unit of the detection device 11.

[0059] The notch 21 extends a certain distance in the circumference, and multiple detection units are arranged laterally at intervals within the notch 21, with a certain distance between them and the inner edge of the notch 21.

[0060] The cultivation device includes a seed tank 14 for cultivating adventitious roots. A heat transfer structure 2 is fitted onto the seed tank 14. The first annular sleeve 22 and the second annular sleeve 23 of the heat transfer structure 2 are respectively fitted onto the outer periphery of the connecting end of the first tank body 12 and the second tank body 13 of the seed tank 14. The notch 21 of the heat transfer structure 2 extends upward from the lower edge of the second annular sleeve 23 to the first annular sleeve 22.

[0061] The first annular sleeve 22 of the seed tank 14 extends from the upper part of the first tank body 12 of the seed tank 14 to the edge where the first tank body 12 and the second tank body 13 connect. The second annular sleeve 23 of the seed tank 14 extends downward from the lower end of the first annular sleeve 22, slightly exceeding the edge where the second tank body 13 and the first tank body 12 connect, and the thickness of the second annular sleeve 23 of the seed tank 14 gradually increases from top to bottom. The notch 21 of the heat transfer structure 2 of the seed tank 14 extends upward from the lower edge of the second annular sleeve 23 of the seed tank 14 to more than half the axial extension length of the first annular sleeve 22.

[0062] The wall thickness of the second annular sleeve 23 of the seed container 14 is greater than the wall thickness of the first annular sleeve 22.

[0063] The cultivation device also includes a cultivation tank 15. The cultivation tank 15 is used to further cultivate the adventitious roots cultivated in the seed tank 14. The heat transfer structure is sleeved on the cultivation tank 15. The first annular sleeve 22 and the second annular sleeve 23 of the heat transfer structure 2 are respectively sleeved on the outer periphery of the connecting end of the first tank body 12 and the second tank body 13 of the cultivation tank 15. The notch 21 of the heat transfer structure 2 extends upward from the lower edge of the second annular sleeve 23 to the second annular sleeve 23.

[0064] The first annular sleeve 22 of the culture tank 15 extends from the upper part of the first tank body 12 of the culture tank 15 to the edge where the first tank body 12 and the second tank body 13 are connected; the second annular sleeve 23 of the culture tank 15 extends downward from the lower end of the first annular sleeve 22 along the second tank body 13 for more than half the distance of the second tank body 13; the notch 21 of the heat transfer structure 2 of the culture tank 15 extends upward from the lower edge of the second annular sleeve 23 on the culture tank 15 to more than half the axial extension length of the second annular sleeve 23.

[0065] The volume of culture tank 15 is much larger than that of seed tank 14. Specifically, the maximum inner diameter of culture tank 15 is much larger than that of seed tank 14. Seed tank 14 has a volume of 200L, while culture tank 15 has a volume of 1000L and 1500L. The horizontal and vertical extension lengths of the notch 21 in seed tank 14 and culture tank 15 are approximately the same or identical.

[0066] In this invention, the seed tank 14 has a first discharge port 141 at its bottom and a first receiving port 142 on its side wall. The culture tank 15 has a second discharge port 151 at its bottom and a second receiving port 152 on its side wall. The culture device also includes a conveying pipeline 16. The conveying pipeline 16 includes a seed transfer pipe 161 and a seed return pipe 162. The seed transfer pipe 161 is used to transfer the culture medium in the seed tank 14 to the culture tank 15. The seed return pipe 162 is used to transfer the culture medium in the culture tank 15 back to the seed tank 14. The seed transfer pipe 161 is connected between the first discharge port 141 of the seed tank 14 and the second receiving port 152 of the culture tank 15. The seed return pipe 162 is located between the first receiving port 142 of the seed tank 14 and the second discharge port 151 of the culture tank 15. The culture solutions in seed tank 14 and culture tank 15 have different compositions. Adventitious roots first grow in seed tank 14, and after reaching a certain stage, they are transported to culture tank 15 through transplanting tube 161 for the next stage of growth. After growing in culture tank 15 for a period of time, the adventitious roots are then transported back to seed tank 14 through return tube 162 for further cultivation.

[0067] The transplanting tube 161 has a five-way valve at the lower end of the transplanting tube 161, a transplanting valve at the upper end of the transplanting tube 161 near the culture tank 15, a seed tank bottom valve at the bottom of the seed tank 14, a CIP inlet pipe connected to the transplanting tube 161 and a CIP valve on the CIP inlet pipe, and a transplanting tube high-temperature steam inlet valve on the transplanting tube high-temperature steam branch inlet pipe connected to the transplanting tube 161.

[0068] One end of the seed return pipe 162 is connected to the bottom of the culture tank 15, and the other end extends upward and connects to the upper side wall of the seed tank 14. The seed return pipe 162 has a five-way valve at the lower end, a seed return valve at the upper end of the seed return pipe 162 near the seed tank 14, a culture tank bottom valve at the bottom of the culture tank 15, a CIP inlet pipe connected to the seed return pipe 162 and a CIP valve on the CIP inlet pipe, and a seed return pipe high-temperature steam inlet valve is provided on the seed return pipe high-temperature steam branch inlet pipe connected to the seed return pipe 162.

[0069] When it is necessary to transfer ginseng adventitious roots from seed tank 14 to culture tank 15, the transplanting tube 161 needs to be disinfected first. The high-temperature steam inlet valve of the transplanting tube is opened to introduce high-temperature steam into the transplanting tube 161 to disinfect the inside of the transplanting tube 161. When the high-temperature steam is introduced to disinfect the transplanting tube 161, the bottom valve of the seed tank connected to the lower end of the transplanting tube 161 will also be disinfected by introducing high-temperature steam.

[0070] After transplanting is completed, the culture tank 15 will return some of the diluted ginseng adventitious roots to the seed tank 14 through the return tube 162. Before the return, the return tube 162 needs to be sterilized with high-temperature steam. Open the high-temperature steam inlet valve of the return tube set on the high-temperature steam branch inlet pipe of the return tube to introduce high-temperature steam into the return tube 162 to sterilize the inside of the transplanting tube 161. When the high-temperature steam is introduced to sterilize the return tube, the bottom valve of the culture tank connected to the lower end of the return tube 162 will also be sterilized with high-temperature steam.

[0071] The five-way valve is installed on both the seed transfer tube 161 and the return tube 162, and the positions are the same. The five-way valve on the seed transfer tube 161 has five ports. Two of the ports of the five-way valve on the seed transfer tube 161 are connected to the seed transfer tube 161, and the other three ports are connected to the discharge valve, the sewage valve, and the CIP discharge valve connected to the seed transfer tube 161, respectively.

[0072] Two ports of the five-way valve on the seed return pipe 162 are connected to the seed return pipe 162, and the other three ports are connected to the discharge valve, the sewage valve, and the CIP discharge valve connected to the seed return pipe 162.

[0073] like Figure 10As shown, in one embodiment of the present invention, a temperature control system is provided. The temperature control system includes a supply unit, a heat transfer structure 2 covering the outer surface of the culture device, and a conveying unit connecting the supply unit and the heat transfer structure 2. The supply unit carries multiple liquids at different temperatures and introduces and removes them from the heat transfer structure 2 via the conveying unit. The supply unit includes several parallel units, each used to introduce and remove different substances into or from the culture device or the heat transfer structure 2.

[0074] Specifically, the temperature control system also includes a temperature-controlled water tank A3 connected to the supply unit. The temperature-controlled water tank A3 is connected in parallel to the supply unit with the culture device. The supply unit can carry various liquids at different temperatures and introduce them into and out of the temperature-controlled water tank A3 via the conveying unit A2. The water in the temperature-controlled water tank A3 circulates to the heat transfer structure 2 to exchange heat with the culture device and then flows back to the temperature-controlled water tank A3.

[0075] In this embodiment, a temperature-regulating water tank A3 is added to the temperature-regulating system, and both the temperature-regulating water tank A3 and the heat transfer structure 2 are connected to the supply unit. This allows the temperature-regulating water tank A3 and the heat transfer structure 2 to form a loop connected in parallel with the supply unit. The temperature-regulating water tank A3 and the heat transfer structure 2 can simultaneously exchange heat media with the supply unit. Furthermore, the temperature-regulating water tank A3 and the heat transfer structure 2 can be connected in series by the supply unit to form a temperature-regulating loop, allowing direct circulation between the temperature-regulating water tank A3 and the heat transfer structure 2. This ensures that the temperature-regulating loop can independently regulate the temperature of the culture device when the supply source of the temperature-regulating system is damaged or under maintenance, thus improving the fault tolerance of the temperature-regulating system.

[0076] In an embodiment of the present invention, a temperature control system for a culture device is described. The temperature control system is further provided with a temperature-controlled water tank A3 and a heat exchange structure A23.

[0077] Specifically, the temperature-regulating water tank A3 is provided with a water-containing cavity A301 for holding and heating the liquid that can be input into the heat transfer structure 2. In addition, the heat exchange structure A23 is disposed against the water-containing cavity A301 and can exchange heat with the liquid in the water-containing cavity A301, thereby forming a circulation loop between the temperature-regulating water tank A3 and the heat transfer structure 2 that can supply heat to the heat transfer structure 2.

[0078] The water-containing cavity A301 and the heat transfer structure 2 are connected to the supply unit via the conveying unit to form a circulation loop. The conveying unit includes an inlet pipe and an outlet pipe connecting the supply unit and the water-containing cavity A301, and the temperature of the culture device is maintained by the temperature-regulating water tank A3.

[0079] Furthermore, the supply unit includes a hot water supply main pipe E200, a chilled water supply main pipe C200, and a steam supply main pipe B100. Each of them carries hot water, chilled water, and high-temperature steam.

[0080] The conveying unit A2 includes an inlet pipe A24 and an outlet pipe A25 that connect the temperature-regulating water tank A3 to the supply unit. The inlet pipe A24 has several branches that can deliver chilled water and high-temperature steam into the temperature-regulating water tank A3 or the heat exchange structure A23.

[0081] The temperature control system also includes a central control unit. This unit is electrically connected to pneumatic valves connected in series in each branch of the inlet pipe A24, and can adjust the flow rate ratio of chilled water and high-temperature steam supplied to the temperature-controlled water tank A3 and the heat exchange structure A23. The chilled water and high-temperature steam entering the temperature-controlled water tank A3 or the heat exchange structure A23 can exchange heat with the hot water in the water chamber A301, thus ensuring that the hot water supplied to the heat transfer structure 2 meets the requirements of the plant tissue culture device. Furthermore, even when the supply unit cannot directly supply hot water to the heat transfer structure 2, the temperature-controlled water tank A3 can be used to supply hot water to the heat transfer structure 2 to maintain the operation of the culture device.

[0082] In another embodiment, an electric heating structure can be installed in the temperature-controlled water tank A3 to directly heat the liquid in the water-containing chamber A301. Therefore, when hot water cannot be directly supplied to the heat transfer structure 2 from the hot water supply main pipe E200, the hot water supply main pipe E200 and the hot water recovery main pipe can be directly connected to the heat transfer structure 2 and the temperature-controlled water tank A3. In this way, a separate circulation loop is formed between the temperature-controlled water tank A3 and the heat transfer structure 2, enabling the direct supply of hot water from the temperature-controlled water tank A3 to the heat transfer structure 2, ensuring the cultivation device meets its hot water temperature requirements.

[0083] Specifically, during normal operation of the supply unit, the heat transfer structure 2 can be supplied with hot water directly from the hot water supply main pipe E200, or simultaneously with hot water from the temperature-regulating water tank A3. In this case, the temperature of the hot water in the water-containing chamber A301 can be further increased before being supplied to the hot water supply main pipe E200, thus providing temperature compensation for the hot water in the main pipe E200 and allowing for more precise adjustment and control of the culture device's temperature.

[0084] The bioreactor for plant tissues combines a seed container 14 and a culture container 15, which can improve the quality of adventitious roots and provide a suitable growth environment, including the control of factors such as temperature, humidity, and nutrients. By adjusting these environmental factors, the growth and development of adventitious roots can be promoted and protected, and convenient operation can be provided, thereby improving production efficiency and product quality.

[0085] By properly positioning the notch 21 on the heat transfer structure 2 to avoid affecting the detection device 11, the detection device 11 can be prevented from being affected by the heat transfer structure 2 without affecting the detection results, thus increasing the service life of the device.

[0086] Although setting a notch 21 on the heat transfer structure 2 can facilitate installation and avoid equipment damage or heat transfer medium leakage caused by poor sealing between the detection device 11 and the heat transfer structure 2, it will lead to problems such as incomplete coverage of the heat transfer structure 2 and uneven temperature of the culture medium in the culture device.

[0087] To solve the above technical problems, such as Figures 4 to 7 As shown, an air intake structure 3 is installed inside the culture device. The air intake structure 3 can deliver sterile air into the device, causing the culture medium inside the device to flow, maintaining a uniform temperature within the culture medium, and promoting the growth of adventitious roots.

[0088] The second tank 13 at the bottom of the seed tank 14 is provided with at least two sets of air inlet structures 3 for supplying air into the tank, located below the heat transfer structure 2 of the seed tank 14; the air inlet structures 3 of the seed tank 14 are arranged opposite to each other on the peripheral wall of the second tank 13 of the seed tank 14. The second tank 13 at the bottom of the culture tank 15 is provided with at least four sets of air inlet structures 3 for supplying air into the tank, located below the heat transfer structure 2 of the culture tank 15; the air inlet structures 3 of the culture tank 15 are evenly distributed circumferentially on the outer peripheral wall of the second tank 13 of the culture tank 15.

[0089] The air intake structure 3 is detachably connected to the side wall of the second tank 13 of the seed tank 14 and the culture tank 15.

[0090] The air intake structure 3 includes an air guide bracket 31, which is a bent pipe comprising a first straight section 311, a second straight section 312, and a third straight section 313. The first straight section 311 is perpendicular to the second straight section 312, and the second straight section 312 and the third straight section 313 are at an obtuse angle. The transitions between the first straight section 311 and the second straight section 312, and between the second straight section 312 and the third straight section 313, are smooth.

[0091] The first straight section 311 has a flared end. The air intake structure 3 also includes a fixed plate 33 and a fixed ring 32. The outer diameter of the fixed ring 32 is larger than that of the fixed plate 33, and its thickness is 2-5 cm. The first straight section 311 of the air guide bracket 31 is detachably installed on the bottom side wall of the second tank 13 through the interaction of the fixed plate 33 and the fixed ring 32. An air intake rod 34 is fitted around the outer periphery of the third straight section 313. The air intake rod 34 is hollow inside and its surface is made of a microporous material formed by titanium sintering. Gas enters the air guide bracket 31 from the first straight section 311 of the air intake structure 3, passes through the second straight section 312 and the third straight section 313, and then enters the air intake rod 34.

[0092] The first straight section 311 has a flared end that first comes into contact with the air that is about to enter the canister, which can guide the air into the intake structure 3. The air passes through the first straight section 311, the second straight section 312 and the third straight section 313 in sequence and enters the intake rod 34. It enters the canister through the micropores that cover the surface of the intake rod 34.

[0093] In this invention, two sets of air intake structures 3 and two sets of cutting structures 4 are provided inside the seed tank 14. The cutting structures 4 are also located below the notch 21 of the heat transfer structure 2. The air intake structures 3 and the cutting structures 4 are spaced apart, that is, the air intake structures 3 and the cutting structures 4 are arranged adjacent to each other. The two sets of air intake rods 34 are installed on the same plane and parallel to each other. The air intake rods 34 have a certain length, and are arranged such that the middle part of the length of the air intake rod 34 forms a central axis with the center of the bottom side wall of the second tank body 13, and are arranged on both sides of the central axis. There is a preset distance between the air intake rods 34 and the cutting structures 4 in the direction extending from the center of the second tank body 13. This installation method of the air intake rods 34 fully ensures uniform air distribution. The air intake rods 34 extend from the third straight section 313 to more than half the distance between every two air intake structures 3.

[0094] The four sets of air intake structures 3 of the culture tank 15 extend into the interior of the second tank body 13. Each air intake structure 3 has a horizontally positioned air intake rod 34 of a certain length, which delivers sterile air into the interior of the second tank body 13 of the culture tank 15 in the form of air bubbles. The projections of any two opposing sets of air intake rods 34 within the tank body are approximately parallel, and the projections of adjacent sets of air intake rods 34 within the tank body are approximately perpendicular. The four sets of air intake structures 3 of the culture tank 15 extend upwards into the interior of the second tank body 13. One set of air intake structures 3 of the culture tank 15 is located directly below the notch 21, and the air intake structures 3 are circumferentially spaced on the second tank body 13 of the culture tank 15.

[0095] In this invention, the culture tank 15 contains four sets of air intake structures 3 and two sets of cutting structures 4, located at different heights within the bottom sidewall of the second tank body 13. A cutting structure 4 is spaced between every two sets of air intake structures 3. The line connecting the installation positions of one set of cutting structures 4 on the same side of the bottom sidewall of the second tank body 13 and the installation positions of two adjacent sets of air intake structures 3 within the tank body approximately forms an isosceles triangle. The cutting structure 4 is installed higher than the air intake structure 3 in the vertical direction. The four sets of air intake structures 3 are evenly distributed within the bottom sidewall of the second tank body 13, with equal spacing between them. The air intake rods 34 of the air intake structures 3 are kept horizontal and have a certain length. The projections of any two opposing sets of air intake rods 34 within the tank body are approximately parallel, and the projections of two adjacent sets of air intake rods 34 within the second tank body 13 are approximately perpendicular.

[0096] The air inlet structure 3 is positioned at the midpoint between the jacket and the bottom of the second tank 13. This avoids uneven mixing of the culture medium at the outer periphery of the culture device or interference with the operation of the first outlet 141 or the second outlet 151 if the air inlet structure 3 is set too low. It also avoids the air inlet structure 3 being set too high, which would prevent the culture medium below the air inlet structure 3 from being mixed evenly.

[0097] The air intake structure 3 provides better stirring between the adventitious roots and the culture solution in the seed tank 14 and the culture tank 15. Under stirring, the culture solution is thoroughly mixed and heat is exchanged, resulting in a more uniform temperature. Furthermore, the culture solution provides ample sterile air to the tank for the adventitious roots to breathe. A good supply of sterile air promotes the respiration and metabolism of the adventitious roots and their root growth.

[0098] The cutting structure 4 can cut adventitious roots that have grown to a certain number or volume into small segments of 5-15mm in length, so as to avoid the adventitious roots from becoming entangled in the culture device due to excessive length, forming a knotted root system, which would prevent the roots from extending normally and affect the growth and development of the plant.

[0099] The second tank body 13 of the seed tank 14 and the culture tank 15 can be fitted with mounting holes for the air intake structure 3's air guide bracket 31 and mounting holes for the spacer 41 of the cutting structure 4 to pass through, and the two mounting holes have different sizes.

[0100] Figures 8 to 9 As shown, the cutting structure 4 is on the seed tank 14 and the culture tank 15. The cutting structure 4 is set on the bottom side wall of the second tank body 13 of the seed tank 14 and the culture tank 15, and is used to cut the adventitious roots that have grown to a certain number or a certain volume to a length suitable for secondary culture of adventitious roots. Preferably, the adventitious roots are cut into small segments of 5-15 mm.

[0101] In this invention, a spacer 41 is provided between the cutting structure 4 and the mounting hole of the second tank 13. A circular mounting hole is formed on the bottom sidewall of the second tank 13, and the outer circumferential surface of the spacer 41 is cylindrical and has the same diameter as the mounting hole. The spacer 41 is detachably installed in the mounting hole on the sidewall of the second tank 13. One side of the spacer 41 is connected to the shearing part 42, and the other side is connected to the power part 44, serving to separate the shearing part 42 from the outside of the second tank 13. The shearing part 42 is installed on the side of the spacer 41 facing inwards from the second tank 13, and the power part 44 is installed on the side of the spacer 41 facing outwards from the second tank 13. The power part 44 can drive the shearing part 42 to cut adventitious roots. The cross-section of the shearing part 42 is smaller than the cross-section of the spacer 41, thereby allowing the cutting structure 4 to be easily and quickly installed on the second tank 13.

[0102] In one embodiment of the present invention, the cutting structure 4 includes a cutting portion 42, which comprises a pin 421, a first blade 423, and a second blade 424 in contact with the first blade 423. The first blade 423 and the second blade 424 are connected together by the pin 421. The pin 421 is perpendicular to the plane of the first blade 423 and the second blade 424.

[0103] In one connection method: the first blade 423 is fixed to the pin 421. The second blade 424 is rotatable relative to the first blade 423 about the pin 421 as its central axis. The opposite sides of the two blades form continuously opening and closing slits, which can be used to cut adventitious roots.

[0104] Specifically, as the second blade 424 rotates, the edges of the two blades change from an interleaved state to an overlapping state, forming continuously opening and closing cuts on the opposite sides of the two blades.

[0105] In this embodiment, the shearing part 42 includes two blades that can rotate relative to each other, with the sides of the two blades touching each other. This allows the overlapping and intersecting edges of the two blades to form continuously opening and closing cuts. The pressure exerted on the adventitious roots by the cuts ensures that the adventitious roots are completely cut off, avoiding the problem of uncut adventitious roots or even entanglement with the shearing part 42 causing damage to the cutting structure 4. It also ensures the flatness of the adventitious root cuts, preventing the adventitious roots from dying after being cut, which is beneficial to improving the growth rate and quality of the adventitious roots.

[0106] In one embodiment of the present invention, a cutting portion 42 capable of cutting out adventitious roots of the same length is described.

[0107] The shearing section 42 includes multiple blade sets 422 connected in series on the pin 421. The multiple blade sets 422 are distributed at equal intervals along the axial direction of the pin 421, forming a multi-layer structure. Each blade set 422 is composed of two first blades 423 and two second blades 424 mating together.

[0108] Specifically, in the blade assembly 422, all the first blades 423 are parallel to each other and fixed to the pivot, and all the second blades 424 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 423. The second blades 424 rotate relative to the first blades 423 to form continuously opening and closing cuts.

[0109] In this way, when the second blade 424 is driven to rotate relative to the first blade 423, the adjacent blade groups 422 simultaneously cut the adventitious roots that enter the shearing section 42, so that the length of the cut adventitious roots is consistent with the interval between the adjacent blade groups 422.

[0110] In this embodiment, the shearing part 42 is provided with multiple sets of blades, and the blade sets 422 are arranged at fixed intervals, so that the adventitious roots can be cut to obtain adventitious roots of uniform length, so that the adventitious roots in the tank have the same growth state, which facilitates the periodic adjustment of the nutrient content of the nutrient solution and the pruning of adventitious roots, promotes the circulation of nutrient solution in the tank, maintains stable growth conditions, and improves the cultivation efficiency and the quality of adventitious roots.

[0111] In another embodiment of the present invention, a shearing section 42 provided with two blade sets 422 is described.

[0112] Two first blades 423 are spaced apart and fitted onto the pin 421 and are arranged parallel to each other. Two second blades 424 abut against the outer side of the first blades 423 opposite to the space.

[0113] The two second blades 424 are arranged in parallel and connected at their ends along their length to form a frame structure. The second blades 424 can be embedded inside the first blade 423 or can be wrapped around the first blade 423 from the outside.

[0114] Preferably, the second blade 424 covers the outside of the first blade 423, that is, it is attached to the side of the first blade 423 that is opposite to the gap.

[0115] In this embodiment, the shearing part 42 is provided with two blade groups 422, and the second blades 424 are connected as a whole. It can be connected from either side of the shearing part 42 and the two second blades 424 can be driven to rotate simultaneously. This simplifies the structure of the shearing part 42, so that the shearing part 42 can not only cut out adventitious roots of the same length, but also keep the cuts of the two blade groups 422 opening and closing synchronously, thus improving the efficiency of trimming adventitious roots.

[0116] In another embodiment of the present invention, a second blade 424 of a shearing section 42 is provided. The second blade 424 includes a rotating end 425 and a blade end 426.

[0117] One end of the blade end 426 is connected to the rotating end 425, and the other end extends along the diameter of the rotating end 425 and is suspended on the outer circumference to form a cantilever.

[0118] The rotating end 425 is sleeved on the pin 421. When the second blade 424 rotates relative to the first blade 423, the blade end 426 can rotate around the pin 421 to form a continuously opening and closing cut.

[0119] In another embodiment of the invention, a shearing section 42 capable of forming curved cuts is described. The first blade 423 and the second blade 424 have the same structure and shape, that is, both are composed of a rotating end 425 and a blade end 426.

[0120] The blade end 426 includes a closed side 427 for cutting and pressing and an extended side 428 opposite to the closed side 427. The closed side 427 and the extended side 428 extend radially from the rotating end 425 to the other end and gradually approach each other.

[0121] In another embodiment of the invention, a shearing section 42 capable of gathering adventitious roots is described. The two ends of the closed side 427 are located on the same diameter line. Furthermore, the closed side 427 extends along an arc from the rotating end 425 to the other end. Finally, an inwardly recessed notch is formed on the closed side 427 of the blade end 426.

[0122] When the second blade 424 rotates relative to the first blade 423, the cut gradually closes from both ends of the closed side 427 toward the notch.

[0123] Furthermore, the second blade 424 includes a plurality of blade ends 426 evenly distributed along the outer periphery of the rotating end 425. For example, three blade ends 426 are arranged at 120° intervals on the outer periphery of the rotating end 425. Four blade ends 426 are arranged at 0° intervals on the outer periphery of the rotating end 425.

[0124] Preferably, the second blade 424 has two blade ends 426 symmetrically distributed about the rotating end 425.

[0125] Specifically, the unfolded side 428 extends outward along an arc from the rotating end 425, and the closed side 427 also extends outward along an arc from the rotating end 425. Furthermore, the unfolded side 428 and the closed side 427 gradually converge at a point. The second blade 424 is S-shaped and consists of two blade ends 426 connected to the rotating end 425 and spaced 180° apart.

[0126] In another embodiment of the invention, a shear section 42 is provided that can promote the circulation of adventitious roots. The blade tip 426 gradually curves upward along the circumferential direction from the closed side 427 to the unfolded side 428 to form a curved surface.

[0127] Preferably, the blade end 426 is configured as a helical surface along the diameter direction of the rotating end 425.

[0128] In another embodiment, to better guide the adventitious roots to circulate within the container, the first blade 423 is elongated. Furthermore, the first blade 423 has pointed angles at both ends that are inclined to its diameter. The side of the pointed angle opposite to the closed side 427 is recessed inwards from the first blade 423.

[0129] In another embodiment of the present invention, a cutting structure 4 for use inside a can is described.

[0130] The cutting structure 4 is located in the inverted conical part, that is, the spacer 41 is located at the bottom of the tank.

[0131] In this embodiment, the cutting structure 4 is placed at the bottom of the tank, which can quickly cut the adventitious roots, making the cuts of the adventitious roots in the tank flat and with the same growth state. This facilitates the regular pruning of the adventitious roots in the tank, promotes the circulation of nutrient solution in the tank, maintains good growth conditions for the adventitious roots, and improves the efficiency and quality of the cultivation of adventitious roots in the tank.

[0132] In another embodiment of the invention, some cutting structures 4 are described inside the can. The cutting structures 4 are detachably connected to the can wall inside the can.

[0133] Specifically, the cutting structure 4 includes a shearing part 42 and a power part 44. The power part 44 can drive the shearing part 42 to cut adventitious roots. The power part 44 and the shearing part 42 are not directly connected. The shearing part 42 is located inside the can body cavity, while the power part 44 is located outside the can. The power part 44 and the shearing part 42 are separated by the can wall inside the can.

[0134] Specifically, a spacer 41 is also provided inside the tank. A circular mounting hole is provided in the tank wall inside the tank, and the outer circumference of the spacer 41 is cylindrical and has the same diameter as the mounting hole. The spacer 41 is detachably fitted into the mounting hole on the tank wall.

[0135] One side of the spacer 41 is connected to the shearing part 42, and the other side is connected to the power part 44, serving to separate the shearing part 42 and the power part 44 inside and outside the tank. The shearing part 42 is installed in the spacer 41 on the side facing the tank cavity, and the power part 44 is installed on the spacer 41 on the side facing the inside and outside of the tank. By making the cross-section of the spacer 41 larger than the cross-section of the shearing part 42, the shearing part 42 can be directly inserted through the mounting hole, thereby allowing the cutting structure 4 to be easily and quickly installed inside the tank.

[0136] The spacer 41 has various styles. In some embodiments of the present invention, the spacer 41 is annular and has an annular groove. When the spacer 41 is annular, the middle part of the spacer 41 is a thin-walled plate and an annular groove is formed by recessing into one side of the plate surface on the outer circumference of the thin-walled plate.

[0137] In other embodiments, the spacer 41 is cylindrical and has a cylindrical groove.

[0138] The cavity opening of the spacer 41 is always located on the end face of the spacer 41. In some embodiments, the spacer 41 is integral with the tank wall and is formed by the tank wall recessing into the cavity of the tank body. In this case, the groove opening of the spacer 41 is formed on the surface of the tank body.

[0139] In other embodiments, the spacer 41 is a separate part that is separable from the tank wall. The spacer 41 can be detachably mounted to the tank wall by fasteners, or it can be fixed to the tank wall by means of bonding or welding.

[0140] In this embodiment, a cutting structure 4 capable of cutting adventitious roots is provided inside the tank, and a spacer 41 is also provided between the cutting part 42 and the power part 44, so that the cutting structure 4 is distributedly installed inside and outside the tank wall. This allows the length of the adventitious roots to be controlled by the cutting structure 4 to maintain and promote the cultivation efficiency of adventitious roots, and also prevents microorganisms or impurities from entering the tank through the cutting structure 4, thus improving the sealing of the tank.

[0141] In another embodiment of the present invention, a spacer 41 for a cutting structure 4 is described. This spacer 41 enables the power unit 44 to better attract and drive the cutting unit 42 to perform the cutting action from one side of the spacer 41, which simplifies the structure of mutual attraction between the cutting unit 42 and the power unit 44, and increases the attraction force of the power unit 44 on the cutting unit 42.

[0142] Specifically, the spacer 41 protrudes from the surface of the tank wall into the tank cavity along the vertical direction of the inner tank wall. Viewed from the outside of the inner tank wall, corresponding to the position where the spacer 41 protrudes into the tank cavity, the spacer 41 forms a groove on the other side.

[0143] Specifically, the shearing part 42 is connected to the outer convex surface of the spacer 41, and the power unit 44 is installed in the groove. Preferably, the shearing part 42 is provided with a component that is sleeved on the outer periphery of the spacer 41 and can rotate relative to the spacer 41. The power unit 44 attracts and drives the component from the other side of the spacer 41 through the spacer 41, thereby driving the shearing part 42 to cut the adventitious root.

[0144] In this embodiment, by optimizing the shape of the spacer 41 so that the spacer 41 protrudes into the cavity of the tank, the attraction and driving effect of the power unit 44 on the shearing unit 42 can be enhanced, ensuring that the cutting structure 4 can better cut the adventitious roots, avoid the adventitious roots from tangling and clumping, and thus promote the faster growth of the adventitious roots.

[0145] In another embodiment of the present invention, a spacer 41 of another cutting structure 4 is introduced. Unlike the spacer 41 in the previous embodiment, the outer circumference of the spacer 41 is provided with an annular groove recessed along its central axis, and the middle part of the spacer 41 is located between the bottom and top of the groove in the direction of the central axis of the annular groove.

[0146] Preferably, the middle part of the spacer 41 is flush with the top of the tank, that is, the middle part of the spacer 41 is flush with the surface of the tank wall inside the tank.

[0147] Specifically, when the spacer 41 is installed inside the tank, the annular groove of the spacer 41 extends from the surface of the tank wall to the tank cavity along the vertical direction of the tank wall inside the tank, and the groove opening of the annular groove is located on the surface of the tank wall inside the tank, so that the spacer 41 forms an annular structure with an annular groove.

[0148] Preferably, the spacer 41 protrudes into the cavity of the tank at its center to form a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 41 so that it protrudes into the cavity of the tank, the attraction and driving effect of the power unit 44 on the shearing unit 42 can be enhanced, ensuring that the cutting structure 4 can better shear the adventitious roots, avoid the adventitious roots from tangling and clumping, and thus promote the faster growth of the adventitious roots.

[0149] In another embodiment of the present invention, a spacer 41 is described that can be detachably installed inside a can. An installation hole is provided on the can wall. If the spacer 41 is annular, it is fixed to the installation hole by embedding its annular groove into the can cavity; if the spacer 41 is barrel-shaped, it is fixed to the installation hole by embedding its bottom into the can cavity.

[0150] Specifically, an installation hole is made in the tank wall inside the tank, and the outer circumferential surface of the spacer 41 is set to be cylindrical and the same diameter as the installation hole. The spacer 41 is detachably fitted into the installation hole in the tank wall.

[0151] In this embodiment, the spacer 41 is connected to the shearing part 42 on one side and to the power part 44 on the other side, separating the shearing part 42 and the power part 44 inside and outside the can. The shearing part 42 is installed inside the can cavity, and the power part 44 is installed inside and outside the can. The shearing part 42 can be directly inserted into the can cavity through the mounting hole, thereby allowing the cutting structure 4 to be easily and quickly installed inside the can.

[0152] In another embodiment of the present invention, an installation position of the tank and the spacer 41 is described.

[0153] The interior of the tank is shaped like a body of revolution, with its central axis running vertically. The tank wall includes at least a portion extending downwards and gradually sloping towards the central axis of the tank. This portion forms an inverted cone shape.

[0154] Preferably, the tank wall also includes a cylindrical portion parallel to the central axis. The cylindrical portion and the inverted conical portion are connected to form the entire interior of the tank, with the inverted conical portion located below and serving as the tank bottom. The spacer 41 is disposed on the inverted conical portion, that is, the spacer 41 is disposed on the tank bottom, thereby distributing the cutting structure 4 at the tank bottom inside the tank.

[0155] In this embodiment, the tank has an inverted conical bottom, which reduces the pressure of the nutrient solution on the adventitious roots and promotes their growth. Furthermore, the inverted conical bottom helps to gather the adventitious roots suspended in the nutrient solution, allowing the spacer 41 and the cutting structure 4 to be installed on the inverted conical bottom, ensuring that the cutting structure 4 can efficiently and effectively shear the adventitious roots.

[0156] In other embodiments of the present invention, a cutting section 42 of a cutting structure 4 is described. The cutting section 42 includes a first blade 423 and a second blade 424. The first blade 423 and the second blade 424 are disposed close together and connected in series by a rotating shaft perpendicular to the contact surface. Both the first blade 423 and the second blade 424 can rotate freely about the rotating shaft, and the opposite sides of the two blades form continuously opening and closing slits, which can be used to cut adventitious roots.

[0157] Another preferred connection method is that the first blade 423 is fixedly connected, and the second blade 424 rotates relative to the first blade 423 around the pivot.

[0158] More preferably, the first blade 423 is fixedly connected to the spacer 41, and the second blade 424 is sleeved on the spacer 41 and can rotate relative to the central axis of the spacer 41. Meanwhile, the power unit 44 is equipped with a rotor that performs circular motion within the groove. A magnetic attractor is mounted on the rotor, which can attract the second blade 424 through the spacer 41.

[0159] In this embodiment, the shearing part 42 is composed of two blades that can rotate relative to each other, and the two blades form a continuously opening and closing slit. The adventitious root is cut through the slit to make the adventitious root cut flush, avoiding dragging and tearing of the adventitious root, improving the success rate of adventitious root cutting, preventing the adventitious root from dying after being cut, and improving the yield and quality of adventitious roots.

[0160] In another embodiment of the present invention, a cutting portion 42 capable of cutting out adventitious roots of a fixed length is described.

[0161] The shearing section 42 includes at least two blade sets 422 spaced apart in the axial direction. Each blade set 422 includes a first blade 423 and a second blade 424, with their sides in contact. The spacing between adjacent blade sets 422 is between 10 mm and 15 mm.

[0162] Specifically, in the blade assembly 422, all the first blades 423 are parallel to each other and fixed to the pivot, and all the second blades 424 are parallel to each other and connected at their ends along their own length to form a frame covering the outside of the first blades 423. The second blades 424 rotate relative to the first blades 423 to form continuously opening and closing cuts.

[0163] In this way, when the second blade 424 is driven to rotate relative to the first blade 423, the adjacent blade groups 422 simultaneously cut the adventitious roots that enter the shearing section 42, so that the length of the cut adventitious roots is consistent with the interval between the adjacent blade groups 422.

[0164] In this embodiment, the shearing part 42 is provided with multiple sets of blades, and the blade sets 422 are arranged at fixed intervals, so that the adventitious roots can be cut to obtain adventitious roots of uniform length, so that the adventitious roots in the tank have the same growth state, which facilitates the periodic adjustment of the nutrient content of the nutrient solution and the pruning of adventitious roots, promotes the circulation of nutrient solution in the tank, maintains stable growth conditions, and improves the cultivation efficiency and the quality of adventitious roots.

[0165] In another embodiment of the present invention, a cutting structure 4 for use inside a can is described. The cutting structure 4 further includes a transmission part 43 between the shearing part 42 and the power part 44.

[0166] Specifically, the transmission part 43 is cylindrical and is sleeved on the spacer 41. The inner cylindrical surface of the transmission part 43 and the outer peripheral surface of the spacer 41 can be slidably connected or rotatably connected and connected together by bearings. In particular, the transmission part 43 can rotate around the spacer 41. The transmission part 43 is connected to the second blade 424 and rotates synchronously, and the power part 44 drives the shearing part 42 to cut the adventitious roots through the transmission part 43.

[0167] In another embodiment of the present invention, a flow guide 45 for a cutting structure 4 is described. A cutting structure 4 is provided inside the tank. In order to better promote the circulation of adventitious roots in the tank cavity and avoid the cutting structure 4 repeatedly cutting a portion of the adventitious roots, a flow guide 45 for guiding the flow of liquid is also provided on the cutting structure 4.

[0168] Specifically, the flow guide 45 is a cylindrical thin-walled tube with cavities extending through both ends along its central axis.

[0169] A flow guide shroud 45 is fitted around the outer periphery of the shearing section 42. One end of the flow guide shroud 45 protrudes from the shearing section 42 and has an inlet 451 on its end face. The inlet 451 is parallel to the circular surface formed by the rotation of the second blade 424. The other end of the flow guide shroud 45 extends toward the tank wall and has an outlet 453 on its end face. The outlet 453 is fitted around the outer periphery of the transmission section 43, such that the flow guide shroud 45 at least partially covers the transmission section 43.

[0170] In this embodiment, by providing a flow guide hood 45 around the outer periphery of the shearing section 42, the circulation flow area of ​​the nutrient solution inside the tank is increased, which promotes the large-scale circulation of adventitious roots in the tank cavity and avoids the adventitious roots in the vicinity of the shearing section 42 from circulating only within a small range due to the influence of the shearing section 42. This ensures that the tank is in a good circulation state, thereby ensuring that all adventitious roots can be pruned.

[0171] The seed tank 14 and the culture tank 15 of the present invention have at least two sets of cutting structures 4 on the bottom side wall of the second tank body 13. The two sets of cutting structures 4 are symmetrically arranged on both sides of the bottom side wall of the second tank body 13 with the center of the bottom of the second tank body 13 as the center, and the distance between the installation positions of the two sets of cutting structures 4 and the center of the bottom of the second tank body 13 is equal. One end of the cutting structure 4 with the power part 44 extends outward to the outside of the second tank body 13, and one end with the shearing part 42 extends inward toward the central axis of the second tank body 13, and is nearly perpendicular to the position formed by the bottom side wall of the second tank body 13.

[0172] The introduction of sterile air into the second tank 13 reduces the liquid density at the bottom of the tank. Air bubbles generated by the air inlet rod 34 disperse the adventitious roots at the bottom of the second tank 13, preventing them from becoming entangled or intertwined. The air inlet structure 3 provides an upward buoyancy when supplying sterile air into the second tank 13. This buoyancy causes the liquid at the bottom to flow, raising the adventitious roots towards the center of the culture device. Once the adventitious roots reach a certain height, they naturally flow back to the bottom of the second tank 13 due to gravity. This process causes the adventitious roots to tumble and move within the culture device. During this tumbling process, the adventitious roots are attracted by the attractive force generated by the rotation of the shearing section 42 of the cutting structure 4.

[0173] When the shearing part 42 inside the cutting structure 4 rotates, it will cause the blade assembly 422 inside the shearing part 42 to rotate rapidly. The fluid flow force generated by the rotation of the blade assembly 422 will cause a low-pressure area to be formed around the cutting structure 4, and a high negative pressure to be generated around the shearing part 42. When the adventitious root approaches the shearing part 42, it will be attracted by the shearing part 42 and sheared.

[0174] The installation method of air intake structure 3 and cutting structure 4 can better trim and maintain adventitious roots, provide sufficient sterile air for adventitious roots, and better control the length of adventitious roots, avoiding excessively long and dense adventitious root systems that are not conducive to material discharge and processing.

[0175] The air intake structure 3 is connected to an air filter via an air intake pipe. A steam branch pipe is connected to the air intake pipe, and steam is introduced into the air intake structure 3 through the steam branch pipe. High-temperature steam is released from the micropores of the air intake structure 3 and floats to the surface of the nutrient solution inside the culture tank 15 near the conical bottom sidewall, causing the nutrient solution near the sidewall to flow upward, while the nutrient solution near the center flows downward.

[0176] The heat transfer structure 2 includes a heat insulation layer 24, which is disposed outside the culture device. The heat insulation layer 24 forms a cavity with the outer wall of the culture device. The cavity has a first opening 241 and a second opening 242, which pass through the heat insulation layer 24 and communicate with the outside, respectively for inputting heat transfer medium into the cavity and discharging heat transfer medium from the cavity. The first opening 241 is disposed on the end of the heat insulation layer 24 with the notch 21. The second opening 242 is disposed on the end of the heat insulation layer 24 opposite to the end with the notch 21.

[0177] The insulation layer 24 contains insulation material, and a flow guide plate is installed in the cavity formed by the insulation layer 24 and the outer wall of the culture device to guide the heat transfer medium and transfer heat to the culture device. During the culture process, the flow guide plate plays a role in guiding the heat transfer medium, increasing the heat exchange area, improving heat transfer efficiency, and preventing dead zones and mixing. It can optimize the heat transfer performance of the heat transfer structure 2 and improve the efficiency and stability of the culture device.

[0178] The insulation layer 24 enhances the incubation device's resilience to external temperature changes. When the external temperature fluctuates, the insulation layer 24 slows down the rate of temperature change. Furthermore, introducing a heat transfer medium into the cavity formed by the insulation layer 24 and the incubation device allows for better temperature control within the device. The piping system enables rapid introduction and discharge of the heat transfer medium into and out of the cavity, thereby accelerating the heat transfer process and improving heat transfer efficiency.

[0179] In this invention, a heat transfer medium is input into the cavity through the first port 241 and output through the second port 242. The temperature within the cultivation device can be adjusted by inputting heat transfer media of different temperatures through the first port 241. When the cultivation device requires heat transfer from the heat transfer structure 2, the heat transfer medium enters the cavity through the first port 241. When the adventitious roots grow to the next stage requiring a different ambient temperature, the heat transfer medium is discharged through the second port 242. Once all the heat transfer medium has been discharged, new heat transfer medium enters through the first port 241, thereby providing the most suitable temperature for the adventitious roots at different growth stages.

[0180] The present invention includes a water tank 5 outside the bioreactor of plant tissue. The water tank 5 is connected to an inlet pipe 52 and an outlet pipe 51. The heat transfer medium is input into the culture device through the inlet pipe 52, and the input heat transfer medium is recovered into the outlet pipe 51 through the second port 242.

[0181] Water has high thermal conductivity, enabling rapid heat transfer. When water flows within a cavity, it quickly absorbs or releases heat, achieving a highly efficient heat transfer process. Water's properties also give it excellent heat capacity and thermal stability. By adjusting the water's temperature and flow rate, the temperature within the cultivation device can be precisely controlled, providing a stable growth environment.

[0182] Since the first inlet 241 is located at the bottom of the cavity, the water flow will impact the cavity from bottom to top, which can effectively clean the surface of the cavity, wash away the dirt on the cavity, ensure the maximum heat transfer effect, and extend the service life of the cavity.

[0183] The notch 21 extends circumferentially, and the detection device 11 includes a temperature sensor, a pH detection device, a dissolved oxygen detection device, and a sampling device. The detection device 11 is circumferentially spaced on the tank within the notch 21.

[0184] The detection device 11 is directly installed on the tank wall and includes temperature measurement, pH measurement, dissolved oxygen measurement, and sampling devices. The detection device 11 allows for real-time monitoring of temperature changes and nutrient parameters inside the cultivation device, helping to adjust the nutrient supply in a timely manner. This ensures that adventitious roots receive sufficient nutrient support for normal growth, improving yield and quality. It also ensures that the temperature remains stable within a suitable range, avoiding the negative impact of temperature fluctuations on adventitious root growth.

[0185] The detection device 11 includes multiple detection units, which are located in the notch 21 at lateral intervals. The lateral extension length of the notch 21 on the culture tank 15 is basically the same as the lateral extension length of the notch 21 on the seed tank 14.

[0186] The detection device 11 is located in the middle part of the culture medium, extending 10 centimeters inward from the tank wall. Since the cutting structure 4 and the air intake structure 3 can make the culture medium form a swirling vortex, the part of the culture medium detected by the detection device 11 is the most uniformly mixed part of the culture medium, ensuring the accuracy of the detection results and improving the culture efficiency.

[0187] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present invention. The implementation schemes in the above embodiments can also be further combined or replaced. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A bioreactor for plant tissue, characterized in that: include, A culture device, including a seed container and a culture container, is used for the culture of plant tissues. A detection device is provided on the outer peripheral wall of the culture device for detecting parameters of the culture medium inside the culture device and / or taking samples. The heat transfer structure is a jacket fitted on the outer peripheral wall of the culture device for temperature control of the culture device. The lower end of the jacket has a notch that extends downward through the lower edge of the jacket. The detection device is located on the outer peripheral wall of the culture device within the notch. The culture device has a first tank and a second tank connected together, the second tank being an inverted cone shape; The second tank at the bottom of the seed tank is equipped with two sets of air intake structures for supplying air into the tank. The air intake structures are located below the heat transfer structure of the seed tank. The air intake structures of the seed tank are arranged opposite to each other on the circumferential wall of the second tank. The two sets of air intake structures of the seed tank extend into the interior of the second tank. The parts of the two sets of air intake structures that extend into the tank have horizontal air intake rods of a certain length, which deliver sterile air into the interior of the second tank in the form of microbubbles. One set of air intake structures for the seed tank is located directly below the notch, and the other set of air intake structures is positioned opposite to the air intake structure below the notch. The second tank at the bottom of the culture jar is equipped with four sets of air intake structures for supplying air into the tank. The air intake structures are located below the heat transfer structure of the culture jar. The four sets of air intake structures extend upwards into the second tank. The part of the air intake structure that extends into the tank has a horizontal air intake rod of a certain length, which delivers sterile air into the second tank of the culture jar in the form of bubbles. The projections of any two opposing sets of air intake rods inside the culture jar are approximately parallel, and the projections of two adjacent sets of air intake rods inside the tank are approximately perpendicular. One set of air intake structures is located directly below the notch, and the air intake structures are circumferentially spaced on the second tank of the culture jar. At least two sets of cutting structures are provided on the seed tank, and the air intake structure and the cutting structure are arranged alternately on the seed tank. At least two sets of cutting structures are provided on the culture tank. The installation position of the cutting structure on the culture tank and the line connecting the installation positions of the two adjacent air inlet structures approximately form an isosceles triangle. The installation position of the cutting structure is higher than the air inlet structure in the vertical direction. The air intake structure includes: a fixed plate, which is rotatably installed at the bottom of the culture tank and seed tank; an air guide bracket, which is installed on the fixed plate; and an air intake rod, which is sleeved on the other end of the air intake pipe for supplying air into the tank. The air guide bracket includes a first straight section, a second straight section, and a third straight section. The first straight section is perpendicular to the second straight section, and the second straight section and the third straight section are set at an obtuse angle. The first straight section is provided with a flared opening, and the third straight section is fitted with an air intake rod. The first straight section and the second straight section, as well as the second and third straight sections, are smoothly transitioned.

2. The bioreactor for plant tissue according to claim 1, characterized in that: The heat transfer structure includes a first annular sleeve and a second annular sleeve that are connected and are respectively sleeved on the outer periphery of the connection end of the first tank and the second tank. The notch extends upward from the lower edge of the second annular sleeve and is provided at least on the second annular sleeve.

3. A bioreactor for plant tissue according to claim 2, characterized in that: The first annular sleeve of the seed container extends from the upper part of the first container body to the edge where the first container body and the second container body are connected; the second annular sleeve of the seed container extends downward from the lower end of the first annular sleeve to slightly beyond the edge where the second container body and the first container body are connected, and the thickness of the second annular sleeve of the seed container gradually increases from top to bottom. The notch in the heat transfer structure of the seed tank extends upward from the lower edge of the second annular sleeve on the seed tank to more than half the axial extension length of the first annular sleeve.

4. A bioreactor for plant tissue according to claim 2 or 3, characterized in that: The culture tank is circulated with the seed tank through a transfer tube and a return tube. The heat transfer structure is sleeved on the culture tank. The first annular sleeve and the second annular sleeve of the heat transfer structure are respectively sleeved on the outer periphery of the connection end of the first tank body and the second tank body of the culture tank. The maximum inner diameter of the first tank body of the culture tank is much larger than the maximum inner diameter of the first tank body of the seed tank. The notch of the heat transfer structure extends upward from the lower edge of the second annular sleeve onto the second annular sleeve.

5. A bioreactor for plant tissue according to claim 4, characterized in that: The first annular sleeve of the culture tank extends from the upper part of the first tank body to the edge where the first tank body and the second tank body connect; The second annular sleeve of the culture tank extends downward from the lower end of the first annular sleeve along the second tank body for more than half the axial extension length of the second tank body; The notch in the heat transfer structure of the culture tank extends upward from the lower edge of the second annular sleeve on the culture tank to more than half the axial extension length of the second annular sleeve.

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