Application of 2-amino-3-methylhexanoic acid as a plant pesticide damage mitigation agent

Abstract

The present invention discloses the application of 2-amino-3-methylhexanoic acid as a plant drug damage mitigation agent. The application of 2-amino-3-methylhexanoic acid in the preparation of a plant drug damage mitigation agent, wherein the concentration of 2-amino-3-methylhexanoic acid described in the preparation is 0.1 to 10 μM. The present invention finds that 2-amino-3-methylhexanoic acid can effectively alleviate plant drug damage and increase plant yield by soaking seeds, and alleviates the damage of fluazifop-pentyl-cypermethrin and cypermethrin-ethyl to rice seeds by soaking seeds at a concentration of 1 μM, thereby achieving the purpose of strengthening seedlings; when the concentration is 1 μM, it can alleviate the drug damage of isoproturon to wheat seeds, and significantly improve the germination rate, bud length and root length of wheat seeds. 2-amino-3-methylhexanoic acid is a natural product, has a low dosage, is environmentally friendly, can effectively alleviate plant drug damage, and is therefore a green and efficient biogenic plant drug damage mitigation agent, which indicates the utilization value and application prospects of such substances in agricultural production.

Description

Technical Field

[0001] The invention belongs to the field of agricultural biological pesticides and relates to the application of 2-amino-3-methylhexanoic acid as a plant pesticide damage alleviating agent. Background Art

[0002] In the actual growth process of agriculture, crops face many threats from pests and diseases. In order to ensure the yield and quality of crops, the use of pesticides is essential. However, some important pesticides have potential damage to crops, which poses a threat to agricultural production safety that cannot be ignored.

[0003] Rice bakanae disease, also known as leggy growth disease, is a very harmful fungal disease. This disease can not only cause a more than 50% reduction in rice yield, but the toxins produced by its pathogen (Gibberella fujikuroi species complex) can also cause food safety problems and affect human health. Since contaminated seeds are the main transmission route of rice bakanae disease, soaking seeds with chemicals is a common, economical and efficient prevention and control measure. At present, the widely used fluazifop-carbamide·carbamide and thiamethoxam·ethionine in production have significant control effects on seedling blight and dry tip nematode disease. However, studies have found that treatment with 800 times diluted 12% fluazifop-carbamide·carbamide dispersible powder has a greater effect on seed germination and seedling growth of Nanjing 5055; soaking seeds in 88.9 times and 66.7 times diluted 17% thiamethoxam·ethionine has a significant inhibitory effect on rice seed germination and seedling establishment, and soaking seeds in 133.3 times diluted solution significantly inhibits seed germination potential and seedling rate.

[0004] Chemical herbicides play an important role in field weeding and increasing crop yields, but excessive use may affect the growth process of crops. Studies have shown that under the treatment of 50% isoproturon wettable powder at a concentration of 40 mg / L and above, the germination rate, root length, and shoot length of wheat were significantly lower than those of the clear water control. At the same time, some researchers have found that the application of isoproturon will reduce the frost resistance of wheat seedlings, and the greater the amount used, the greater the harm. Especially under low temperature conditions, isoproturon can cause serious pesticide damage to wheat. Therefore, in view of the pesticide damage faced by different crops in actual production, the development of products and technologies aimed at alleviating crop pesticide damage is particularly urgent to ensure agricultural safety production.

[0005] At present, some plant damage mitigators have been discovered, such as brassinosteroids, brassinolide, and aminobutyric acid. Studies have shown that brassinosteroids can alleviate plant damage from pesticides by regulating reactive oxygen species and mitogen-activated protein kinases; spraying brassinolide in the early stage of herbicide damage can increase the thousand-grain weight and number of grains per ear of wheat, thereby increasing wheat yield; γ-aminobutyric acid can not only inhibit the absorption of pesticides by plants by regulating stomatal movement, but also alleviate pesticide damage through reactive oxygen signals. As the international standards for the residue limit of plant chemical reagents are becoming increasingly stringent, it is very important to seek green, efficient, and environmentally friendly plant damage mitigators.

[0006] 2-Amino-3-methylhexanoic acid (AMHA for short), chemical formula is C7H 15 NO2, with a molecular weight of 145 g / mol, is a new type of amino acid compound and is a colorless, transparent crystal. Recently, we successfully isolated and purified 2-amino-3-methylhexanoic acid from fungi and confirmed for the first time that it is a natural amino acid. We conducted a systematic study on its plant immunity and induction activity and found that in terms of resistance to biological stress, 2-amino-3-methylhexanoic acid can effectively inhibit the occurrence and spread of viruses, fungi and bacteria on plant leaves; in terms of inducing plant resistance to abiotic stress, 2-amino-3-methylhexanoic acid can effectively alleviate the damage caused to plants by high temperature, low temperature, drought and salinity; in terms of improving quality, 2-amino-3-methylhexanoic acid can increase the content of amino acids such as theanine in tea; not only that, 2-amino-3-methylhexanoic acid can also promote plant growth and increase yield. The above patents ZL202011549486.6, ZL202110795699.5 and ZL202110975196.6 have been applied for. So far, there is no relevant research, report or patent on the use of 2-amino-3-methylhexanoic acid to alleviate plant pesticide damage, which is the innovativeness of this patent. Summary of the invention

[0007] The object of the present invention is to provide the use of 2-amino-3-methylhexanoic acid as a phytotoxicity alleviating agent in view of the above-mentioned deficiencies in the prior art.

[0008] The purpose of the present invention can be achieved through the following technical solutions:

[0009] The invention discloses an application of 2-amino-3-methylhexanoic acid in preparing a plant drug damage alleviating agent, wherein the concentration of the 2-amino-3-methylhexanoic acid in the preparation is 0.1 to 10 μM.

[0010] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used in the preparation of a phytotoxicity mitigating agent for alleviating the damage of fluazifop-butyl, fluazifop-butyl and fluazifop-butyl to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid in the preparation is preferably 0.1 to 1 μM.

[0011] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used in the preparation of a phytotoxicity alleviating agent for alleviating the damage of cypermethrin and ethionine to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid in the preparation is preferably 0.1-1 μM.

[0012] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used in the preparation of a phytotoxicity alleviating agent for alleviating the damage of isoproturon to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid in the preparation is preferably 1 to 10 μM.

[0013] The gramineous crops described in the present invention include but are not limited to wheat and rice.

[0014] The application of 2-amino-3-methylhexanoic acid in alleviating plant pesticide damage, the concentration of 2-amino-3-methylhexanoic acid is 0.1-10 μM.

[0015] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used to alleviate the damage of fluazifop-butyl, fluazifop-butyl and fluazifop-butyl to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid is preferably 0.1-1 μM.

[0016] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used to alleviate the damage of cypermethrin and ethionine to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid is preferably 0.1-1 μM.

[0017] As a preferred embodiment of the present invention, 2-amino-3-methylhexanoic acid is used to alleviate the damage of isoproturon to seed germination and seedlings of gramineous crops, and the concentration of 2-amino-3-methylhexanoic acid is preferably 1-10 μM.

[0018] The gramineous crops described in the present invention include but are not limited to wheat and rice.

[0019]

[0020] The existing related research of 2-amino-3-methylhexanoic acid shown in formula (I) does not involve reports in the field of plant pesticide harm mitigation agents. Affected by the continental monsoon climate, my country's agricultural pests and diseases are of many types, with a large area of ​​occurrence and severe degree, and chemical pesticides are the current main means. However, excessive application of pesticides not only affects the quality and safety of agricultural products, but also threatens the agricultural ecological environment and human health, and has gradually become one of the main influencing factors restricting the sustainable development of my country's agriculture. Therefore, finding natural, green and efficient plant pesticide mitigation agents and promoting their industrialization are of great significance for ensuring agricultural production safety and improving the competitiveness of agricultural products. 2-amino-3-methylhexanoic acid performs well in related plant pesticide harm mitigation experiments and can help plants alleviate pesticide harm problems.

[0021] The method of using the natural metabolite 2-amino-3-methylhexanoic acid to alleviate plant drug damage, the details and implementation plan are as follows: within the concentration range of 0.1-10 μM, the plant drug damage problem can be effectively alleviated.

[0022] The method of using 2-amino-3-methylhexanoic acid to alleviate the damage of fluazifop-pentyl-carbazine and carbazine-acetophenone to rice is tested by adding pesticides and 2-amino-3-methylhexanoic acid to soak seeds at the concentration range of 0.1-10 μM. The results showed that the germination rate, sprout length and root length of rice seeds in the experimental group were significantly improved compared with the control group without the addition of 2-amino-3-methylhexanoic acid. This result shows that 2-amino-3-methylhexanoic acid can effectively alleviate the damage of these two pesticides to rice seed germination and seedling growth.

[0023] The method of using 2-amino-3-methylhexanoic acid to alleviate the damage of isoproturon to wheat is tested by adding isoproturon and 2-amino-3-methylhexanoic acid to soak seeds at the same time in the concentration range of 0.1-10 μM. The results showed that compared with the control group without the addition of 2-amino-3-methylhexanoic acid, the germination rate, sprout length and root length of wheat seeds in the experimental group were significantly improved. This result shows that 2-amino-3-methylhexanoic acid can effectively alleviate the damage of isoproturon to wheat seed germination and seedling growth.

[0024] Technological advancement and beneficial effects

[0025] The main advantages and positive effects of the present invention are as follows:

[0026] 2-amino-3-methylhexanoic acid is a natural product with a simple structure and is easy to produce industrially. Since the present invention confirms that 2-amino-3-methylhexanoic acid can alleviate plant pesticide damage, it has the potential to be developed into a natural plant pesticide damage alleviating agent.

[0027] The present invention finds that 2-amino-3-methylhexanoic acid can effectively alleviate plant drug damage and increase plant yield by soaking seeds. At a concentration of 1 μM, the damage of fluazifop-butyl-carbazine and carbazine-ethyl-carbazine to rice seeds can be alleviated by soaking seeds, thereby achieving the purpose of strengthening seedlings. At a concentration of 1 μM, the drug damage of isoproturon to wheat seeds can be alleviated, and the germination rate, bud length and root length of wheat seeds can be significantly improved. 2-amino-3-methylhexanoic acid is a natural product with low dosage and is environmentally friendly. It can effectively alleviate plant drug damage and is therefore a green and efficient biological source plant drug damage alleviator, which indicates the utilization value and application prospects of such substances in agricultural production.

[0028] The present invention finds that 2-amino-3-methylhexanoic acid can alleviate the damage of pesticides to plants by soaking seeds. 2-amino-3-methylhexanoic acid is easy to use, solves production problems that cannot be solved by traditional agronomy, and saves production costs. In addition, since 2-amino-3-methylhexanoic acid is a naturally occurring metabolite with a simple structure and belongs to α-amino acid, it has high environmental and biological safety and belongs to the category of green and efficient biochemical pesticides. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Effects of different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides and fluazifop-butyl amine and cypermethrin mixed with seed soaking on rice seed germination and seedling growth

[0030] Figure 2 Effects of mixed seed soaking with gibberellins, fluazifop-butyl, chlorpyrifos and chloranil on rice seed germination and seedling growth

[0031] Figure 3 Effects of different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides and cypermethrin-ethionine mixed with acetophenone on rice seed germination and seedling growth

[0032] Figure 4 Effects of Mixed Seed Soaking with Gibberellic Acid and Cypermethrin on Seed Germination and Seedling Growth of Rice

[0033] Figure 5 Effects of different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides, gibberellins and isoproturon mixed seed soaking on wheat seed germination and seedling growth DETAILED DESCRIPTION

[0034] The inventors have studied the biological activity, application scope and crop safety of 2-amino-3-methylhexanoic acid and found that the substance also has a very unique effect in alleviating plant damage, and has the advantages of being environmentally friendly, widely applicable and safe to use. It is a natural plant damage mitigator and has the potential to be developed into a biological pesticide. The essential features of the present invention can be reflected from the following embodiments and examples, but these should not be regarded as any limitation of the invention.

[0035] Example 1: 2-amino-3-methylhexanoic acid alleviates the damage of fluazifop-butyl, pentamethylenetetracycline and chloranthoxam to rice seed germination and seedlings

[0036] The rice seeds (variety "Dengliangyou 2108") were rinsed with distilled water, disinfected with 75% alcohol for 3 minutes, washed with distilled water 3 times, and then disinfected with NaClO (5%) for about 10 minutes. After taking out, they were rinsed with distilled water, and then the residual water on the surface of the seeds was absorbed with clean filter paper until the surface was dry. 4g (grams) of healthy, plump and uniform seeds were selected and placed in a 50mL (milliliter) centrifuge tube. Clean water, 0.1, 1, and 10μM (micromoles / liter) 2-amino-3-methylhexanoic acid and 8mL of 0.1% amino oligosaccharide aqueous solution were first added to each tube, and then 0.02g of fluazifop-pentyl-carbazine (400 times diluted, F was added to each tube) 400 ). Stir the seeds until they are completely immersed in the solution. After soaking at room temperature for 48 hours, do not rinse and germinate directly. Each group of experiments was repeated three times. For each repeat, 30 rice seeds were randomly selected with tweezers and placed in a culture dish pre-laid with moist filter paper. The dish was placed in a 28°C culture room for germination. After waiting for 3 days, the seed germination rate, bud length and root length were measured and recorded.

[0037] Table 1 Effects of soaking rice seeds with different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides and fluazifop-pentyl-carbazine on the germination rate, sprout length and root length of rice seeds

[0038]

[0039]

[0040] The results showed that soaking rice seeds in a 400-fold dilution of fluazifop-butyl and cypermethrin seriously reduced the germination rate of rice seeds, which was reduced by 16% compared with the water control, and the sprout and root length of the seedlings were reduced by 67% and 64%, respectively. As the concentration of 2-amino-3-methylhexanoic acid added to the soaking solution increased, the damage of fluazifop-butyl and cypermethrin to the germination rate, sprout length and root length of rice seeds gradually decreased (Table 1, Appendix Figure 1). Compared with the treatment with fluazifop-butyl·acetyl·carbazine, the germination rate of rice seeds increased significantly by 26%, 26% and 24% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, and the seed germination rate increased by 17% in the presence of amino-oligosaccharides, but there was no significant difference; the sprout length increased by 200%, 250% and 150% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, respectively, and the sprout length increased by 50% in the presence of amino-oligosaccharides, but there was no significant difference; the root length increased by 140%, 140% and 120% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, respectively, and the root length increased by 60% in the presence of amino-oligosaccharides, but there was no significant difference. Obviously, each treatment concentration of 2-amino-3-methylhexanoic acid can significantly alleviate the phytotoxicity of fluazifop-butyl · pentothion to rice, but the commercial plant immune activator amino oligosaccharide cannot significantly alleviate the phytotoxicity of rice. When the concentration of 2-amino-3-methylhexanoic acid is greater than 1 μM, the phytotoxicity alleviation effect on rice seeds is no longer significantly increased. Therefore, the treatment effect of 2-amino-3-methylhexanoic acid at a concentration of 1 μM is the best, and the germination rate, bud length and root length reach 105%, 117% and 86% of the blank control, respectively.

[0041] Table 2 Effects of seed soaking with 0.1 g / L gibberellin and fluazifop-butyl-carbazine on germination rate, sprout length and root length of rice seeds

[0042]

[0043] In order to determine whether 2-amino-3-methylhexanoic acid can alleviate the phytotoxicity of the rice seed soaking agent fluazifop-butyl due to its growth-promoting activity, gibberellins (0.1 g / L) with growth-promoting function were selected as a positive control for experiments, and the method was the same as above. The results showed that soaking seeds with gibberellins increased the germination rate of rice seeds, which was 8% higher than that of the clear water control, and the seedling sprout length and root length increased by 63% and 5%, respectively. Soaking seeds with a 400-fold diluted solution of fluazifop-butyl seriously reduced the germination rate of rice seeds, which was 7% lower than that of the clear water control, and the seedling sprout length and root length decreased by 13% and 45%, respectively. After adding gibberellins to the seed soaking solution, fluazifop-butyl did not significantly alleviate the damage to the germination rate, sprout length and root length of rice seeds (Table 2, Appendix Figure 2). When 0.1g / L gibberellin was present, the germination rate of rice seeds decreased by 9%, the sprout length increased by 29%, and the root length increased by 8% compared with fluazifop-butyl. However, there was no significant difference. However, when 1μM 2-amino-3-methylhexanoic acid was added, the germination rate of rice seeds increased by 6%, the sprout length increased by 14%, and the root length increased by 42%. Obviously, the use of gibberellin did not significantly alleviate the damage of fluazifop-butyl to rice seeds. The above results show that the seed soaking treatment with 2-amino-3-methylhexanoic acid can effectively alleviate the phytotoxicity problem of the rice seed soaking agent fluazifop-butyl, but it is not due to its immune and growth-promoting activity.

[0044] Example 2: 2-amino-3-methylhexanoic acid alleviates the damage of cypermethrin and ethionine to rice seed germination and seedlings

[0045] Rinse the rice seeds (variety "Dengliangyou 2108") with distilled water, disinfect with 75% alcohol for 3 minutes, wash with distilled water 3 times, and then disinfect the rice seeds with NaClO (5%) for about 10 minutes. After taking them out, rinse them with distilled water, and then use clean filter paper to absorb the residual moisture on the surface of the seeds until the surface is dry. Select 4g (grams) of healthy, plump and uniform seeds and place them in a 50mL (milliliter) centrifuge tube. Add clean water, 0.1, 1, and 10μM (micromoles / liter) 2-amino-3-methylhexanoic acid and 8mL of 0.1% amino oligosaccharide aqueous solution to each tube, and then add 0.053g of thiamethoxam·ethionine (150 times diluted, S 150 ). Stir the seeds until they are completely immersed in the solution. After soaking at room temperature for 48 hours, do not rinse and germinate directly. Each group of experiments was repeated three times. For each repeat, 30 rice seeds were randomly selected with tweezers and placed in a culture dish pre-laid with moist filter paper. The dish was placed in a 28°C culture room for germination. Wait for 2-3 days and record the germination rate, sprout length and root length of the rice seeds.

[0046] Table 3 Effects of soaking seeds with different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides and cypermethrin and ethionine on the germination rate, sprout length and root length of rice seeds

[0047]

[0048] The results showed that soaking rice seeds in 150-fold diluted solution of cypermethrin and ethionine seriously reduced the germination rate of rice seeds, which was reduced by 16% compared with the water control, and the sprout and root length of the seedlings were reduced by 50% and 43%, respectively. As the concentration of 2-amino-3-methylhexanoic acid added to the soaking solution increased, the damage of cypermethrin and ethionine to the germination rate, sprout length and root length of rice seeds gradually decreased (Table 3, Appendix Figure 3). Compared with the treatment of cypermethrin and ethionine, the germination rate of rice seeds increased significantly by 24%, 15% and 11% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, and the seed germination rate increased by 3% in the presence of amino-oligosaccharides, but there was no significant difference; in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, the sprout length increased by 100%, 133% and 200%, respectively, and there was no significant change in the treatment of amino-oligosaccharides; in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, the root length increased by 75%, 75% and 63%, respectively, and there was no significant difference in the treatment of amino-oligosaccharides. Obviously, the concentrations of 2-amino-3-methylhexanoic acid significantly alleviated the phytotoxicity of cypermethrin and ethionine to rice, but the commercial plant immune activator amino-oligosaccharides could not significantly alleviate the phytotoxicity of rice. Moreover, the treatment effect of 2-amino-3-methylhexanoic acid was the best when the concentration was 10 μM, and the germination rate, shoot length and root length reached 92%, 150% and 93% of the blank control, respectively.

[0049] Table 4 Effects of 0.1 g / L GA and cypermethrin·ethionine soaking on rice seed germination rate, sprout length and root length

[0050]

[0051] In order to determine whether 2-amino-3-methylhexanoic acid can alleviate the phytotoxicity of rice seed soaking agent chlorfenapyr due to its growth-promoting activity, gibberellins (0.1 g / L) with growth-promoting function were selected as positive control for experiments, and the method was the same as above. The results showed that soaking seeds with gibberellins increased the germination rate of rice seeds by 8% compared with the clear water control, and the seedling sprout length and root length increased by 63% and 5%, respectively. Soaking seeds with 150-fold diluted chlorfenapyr seriously reduced the germination rate of rice seeds by 10% compared with the clear water control, the seedling sprout length decreased by 75%, and the root length decreased by 36%. After adding gibberellins to the seed soaking solution, the damage of chlorfenapyr to the germination rate, sprout length and root length of rice seeds was not significantly alleviated (Table 4, Appendix Figure 4 ). When 0.1g / L gibberellin was present, the germination rate of rice seeds decreased by 11%, the sprout length did not change significantly, and the root length decreased by 7%, but there was no significant difference; while the addition of 10μM 2-amino-3-methylhexanoic acid increased the germination rate of rice seeds by 5%, the sprout length increased by 33%, and the root length increased by 36%. Obviously, the use of gibberellin did not significantly alleviate the damage of cypermethrin to rice seeds. The above results show that the seed soaking treatment with 2-amino-3-methylhexanoic acid can effectively alleviate the problem of phytotoxicity of rice seed soaking agent cypermethrin.

[0052] Example 3: 2-amino-3-methylhexanoic acid alleviates the damage of isoproturon to wheat seed germination and seedlings

[0053] Rinse the wheat seeds (variety "Yangfu Wheat No. 4") with distilled water, disinfect with 75% alcohol for 3 minutes, wash with distilled water 3 times, and then disinfect the wheat seeds with NaClO (5%) for about 10 minutes. After taking them out, rinse them with distilled water, and then use clean filter paper to absorb the residual moisture on the surface of the seeds until the surface is dry. Select 4g (grams) of healthy, plump and uniform seeds and place them in a 50mL (milliliter) centrifuge tube. Add clean water, 0.1, 1, 10μM (micromoles / liter) 2-amino-3-methylhexanoic acid, 0.1% amino oligosaccharide aqueous solution and 0.1g / L (grams / liter) gibberellin 8mL to each tube, and then add 0.024g isoproturon (3g / L) to each tube. Stir to make the seeds completely immersed in the liquid, soak them for 48 hours, and germinate them directly without rinsing. Each group of experiments was repeated three times. For each repeat, 30 rice seeds were randomly selected with tweezers and placed in a culture dish pre-covered with moist filter paper. The seeds were placed in a 28°C culture room until the wheat seeds turned white. The seeds were placed in a 4°C incubator for dark treatment for 2 days and then germinated in a 28°C culture room. After waiting for about 2 days, the germination rate, sprout length, and root length of the wheat seeds were recorded.

[0054] Table 5 Effects of different concentrations of 2-amino-3-methylhexanoic acid, amino oligosaccharides, gibberellins and isoproturon soaking on wheat seed germination rate, sprout length and root length

[0055]

[0056] The results showed that soaking seeds in dilute isoproturon solution seriously reduced the germination rate of wheat seeds, which was reduced by 81% compared with the water control, and the sprout and root length of seedlings were reduced by 65% ​​and 63%, respectively. As the concentration of 2-amino-3-methylhexanoic acid added to the soaking solution increased, the damage of isoproturon to the germination rate, sprout length and root length of wheat seeds gradually decreased (Table 5, Appendix Figure 5). Compared with the treatment with isoproturon, the germination rate of wheat seeds increased significantly by 308%, 369% and 392% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, and the germination rate increased by 100% in the presence of amino-oligosaccharides; the sprout length increased by 100%, 150% and 150% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, respectively, and the sprout length increased by 17% in the presence of amino-oligosaccharides, but there was no significant difference; the root length increased by 73%, 118% and 127% in the presence of 0.1μM, 1μM and 10μM 2-amino-3-methylhexanoic acid, respectively, and the root length increased by 18% in the presence of amino-oligosaccharides, but there was no significant difference. Obviously, the concentrations of 2-amino-3-methylhexanoic acid can significantly alleviate the phytotoxicity of isoproturon to wheat, but the commercial plant immune activator amino-oligosaccharides cannot significantly alleviate the phytotoxicity of isoproturon to wheat. When the concentration of 2-amino-3-methylhexanoic acid is greater than 1 μM, the mitigation effect on wheat seeds no longer increases significantly. Therefore, the treatment effect of 2-amino-3-methylhexanoic acid at a concentration of 1 μM is the best, and the germination rate, sprout length and root length reach 87%, 88% and 80% of the blank control, respectively. The above results show that 2-amino-3-methylhexanoic acid seed soaking treatment can effectively alleviate the problem of phytotoxicity of wheat seed soaking agent isoproturon.

[0057] In order to exclude the possibility that 2-amino-3-methylhexanoic acid can alleviate the damage of isoproturon dilution to wheat by promoting growth, gibberellin (0.1 g / L) with growth-promoting function was selected as a positive control for the experiment. The results showed that gibberellin seed soaking increased the germination rate of wheat seeds by 7% compared with the water control, the seedling sprout length increased by 18%, and the root length was inhibited by 7%. After adding gibberellin to the isoproturon seed soaking solution, isoproturon had no significant relief on the damage of wheat seed germination rate, sprout length and root length (Table 5, Appendix Figure 5 ). When 0.1g / L gibberellin was present, the germination rate of wheat seeds decreased by 8%, the bud length increased by 33%, and the root length increased by 18% compared with isoproturon, but there was no significant difference. Obviously, the use of gibberellin did not significantly alleviate the damage of isoproturon to wheat seeds. The above results show that 2-amino-3-methylhexanoic acid seed soaking treatment can effectively alleviate the problem of phytotoxicity of isoproturon, a water-based wheat seed soaking agent.

[0058] References:

[0059] [1] Chen Shiguo, Li Jingjing, Wang Lan, Wang He, Qiang Sheng, Fang Wanping, Zhang Yu. Application of 2-amino-3-methylhexanoic acid as plant immune inducer. Chinese invention patent, patent number ZL202011549486.6.

[0060] [2] Chen Shiguo, Li Jingjing, Wang Lan, Wang He, Qiang Sheng, Fang Wanping, Zhang Yu. Application of 2-amino-3-methylhexanoic acid in improving tea quality. Chinese invention patent, patent number ZL202110795699.5.

[0061] [3] Chen Shiguo, Wang He, Tan Weiming, Wang Jing, Li Jingjing, Fang Wanping, Zhang Yu, Qiang Sheng. Application of 2-amino-3-methylhexanoic acid in promoting plant growth and increasing yield. Chinese invention patent, patent number ZL202110975196.6.

[0062] [4] Zhou Bin, Zhang Yajing, Wu Jianming. Effects of 12% fluazifop-pentyl-carbamide and chlorpyrifos on germination of late japonica rice seeds[J]. Agricultural Science and Technology Newsletter, 2023, No.614(02):73-76.

[0063] [5] Gu Donghua. Effects of 17% cypermethrin and ethoxylated WP on germination of rice seeds of “Wuyunjing 19”[J]. Shanghai Agricultural Science and Technology, 2014, (05): 51-55.

[0064] [6] Cui Lina, Jiang Xiaojun, Cui Li. Effect of 50% isoproturon wettable powder on wheat germination[J]. Modern Agricultural Science and Technology, 2017, (11): 110-114.

[0065] [7]Zhou Y,Xia X,Yu G,et al.Brassinosteroids play a critical role in the regulation of pesticide metabolism in crop plants[J].Sci Rep,2015,5:9018.

[0066] [8] Shan Q, Liu M, Li R, et al. γ-Aminobutyric acid (GABA) improve spesticide detoxification in plants [J]. Sci Total Environ, 2022, 835: 155404.

Claims

1. The use of 2-amino-3-methylhexanoic acid in the preparation of a plant phytotoxicity alleviating agent, characterized in that: Application of 2-amino-3-methylhexanoic acid in the preparation of a phytotoxicity mitigating agent for mitigating the damage of fluazifop, pentamethylenetetracycline and chlorpyrifos to seed germination and seedlings of gramineous crops, wherein the concentration of 2-amino-3-methylhexanoic acid in the preparation is 0.1-1 μM; Application of 2-amino-3-methylhexanoic acid in the preparation of a phytotoxicity alleviating agent for alleviating the damage of cypermethrin and ethionine to seed germination and seedlings of gramineous crops, wherein the concentration of 2-amino-3-methylhexanoic acid in the preparation is 0.1-1 μM; The invention discloses an application of 2-amino-3-methylhexanoic acid in the preparation of a phytotoxicity alleviating agent for alleviating the damage of isoproturon to seed germination and seedlings of gramineous crops. The concentration of 2-amino-3-methylhexanoic acid in the preparation is 1-10 μM.

2. The application of 2-amino-3-methylhexanoic acid in alleviating plant pesticide damage is characterized in that: Application of 2-amino-3-methylhexanoic acid in alleviating the damage of fluazifop-butyl, fluazifop-butyl and fluazifop-butyl to seed germination and seedlings of gramineous crops, with the concentration of 2-amino-3-methylhexanoic acid being 0.1 ~1μM; Application of 2-amino-3-methylhexanoic acid in alleviating the damage of cypermethrin and ethionine to seed germination and seedlings of gramineous crops, with a concentration of 2-amino-3-methylhexanoic acid of 0.1 ~1μM; Application of 2-amino-3-methylhexanoic acid in alleviating the damage of isoproturon to seed germination and seedlings of gramineous crops, with the concentration of 2-amino-3-methylhexanoic acid being 1 ~10 μM.

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