Method and device for killing hydroxyl free radicals of parasites in factory-farmed animals

Abstract

The present invention relates to a method and device for killing hydroxyl radicals of parasites in factory-farmed animals. The device is provided with a hydroxyl radical (·OH) solution generating device, a ·OH solution spraying microjet device, a mixing tank, a seawater culture pool, a microfilter / protein separator / biofilter, a sand filter-water reservoir, a real-time online detection system and an intelligent control center. Oxygen active particles efficiently generate ·OH solution through a gas-liquid mixer and a mixing tank, and the ·OH solution water pipe sprays the ·OH solution and cavitation bubbles to the ·OH solution treatment culture pool through a star-shaped ·OH solution microjet spray device, synchronously or intermittently achieving 100% killing of cultured animal parasites and other pathogenic microorganisms in multiple culture pools, and mineralizing organic matter in the culture pool and oxidizing inorganic nitrogen. The method is automated, efficient, green and safe, does not generate disinfection byproducts, purifies culture water, and ensures the growth and health of cultured animals and the safety of marine ecology.

Description

Technical Field

[0001] The invention relates to the fields of plasma chemistry, aquaculture technology and marine environmental engineering technology, and in particular to a method and device for killing hydroxyl free radicals of parasites in factory-farmed animals. Background Art

[0002] With the development of my country's economy, people's living standards have gradually improved, and the demand for seafood has also increased year by year. Factory aquaculture is an intensive type of high-density, high-survival rate, high-yield, and multi-batch farming throughout the year. Compared with offshore cage farming, factory farming has the characteristics of less water consumption, small footprint, high resource utilization, small impact on the environment, controllable product quality, production and farming are not affected or restricted by the environment, and strong resistance to natural risk shocks. How to effectively isolate aquaculture diseases, control the input of pathogens, reduce the number of pathogens carried by farmed fish, shrimp and other aquatic products, and cut off the transmission of pathogens is crucial to the closed factory aquaculture water system.

[0003] Conventional circulating water treatment processes generally include: microfiltration system, foam separation, microbial treatment, and ultraviolet sterilization and disinfection. Microfiltration system and foam separator can only separate solid particles such as feces and leftover bait, while microbial treatment can only remove pollutants such as ammonia nitrogen and nitrite nitrogen. Ultraviolet disinfection technology is widely used in aquaculture water treatment, but its sterilization time is long and has high requirements for water turbidity, which cannot meet the requirements for killing parasites and diseases in factory-scale high-density aquaculture water.

[0004] At present, chemical agents such as formaldehyde, copper sulfate, potassium permanganate, chlorine oxidants, etc. are mostly used to kill farmed fish parasites. Wang Zhonghua et al. (Wang Zhonghua, Zhang Chencang, Wang Yingeng, et al. Cryptocaryon irritans disease and its prevention and control in cage-cultured large yellow croaker [J]. Fisheries Science and Technology Information, 2012, 39(6): 293-298.) used 200 mg / L formaldehyde to bathe the infected large yellow croaker. After 1 hour, the density of Cryptocaryon irritans remained basically unchanged. After 4 hours, the fish's vitality weakened and they stopped eating. Buchmann K et al. (BUCHMANN K, BRESCIANI J, JAPPEC. Effects of formalin treatment on epithelial structure and mucous cell densities in rainbow trout, Oncorhynchus mykiss (Walbaum), skin [J]. Journal of fish diseases, 2004, 27(2): 99-104.) confirmed that formaldehyde can stimulate the fish to secrete a large amount of mucus, aggregate into clumps, and hinder the fish's breathing. Wu Jianshao et al. (Wu Jianshao, Li Leibin, Zhu Zhihuang, et al. Study on the therapeutic effect of copper sulfate on Cryptocaryonosis of Takifugu bimaculatus [J]. Fisheries Science, 2019, 38(3): 305-312.) used 1.5 mg / L copper sulfate to bathe the diseased Takifugu bimaculatus. After 3 days, some trophozoites on the surface of the diseased fish disintegrated or fell off, but long-term exposure to copper ions caused damage to the gills of the fish and even growth inhibition. Wang Zhonghua (Wang Zhonghua, Zhang Chencang, Wang Yingeng, et al. Cryptocaryonosis of large yellow croaker cultured in cages and its prevention and control [J]. Fisheries Science and Technology Information, 2012, 39(6): 293-298.) et al. used 2 mg / L potassium permanganate to bathe the diseased large yellow croaker. After 1 hour, the trophozoite density on the surface of the diseased fish decreased, but the diseased fish died after 4 hours, and there were still live parasites.Hirazawa (HIRAZAWA N, GOTO T, SHIRASU K. Killing effect of various treatments on the monogenean Heterobothrium okamotoi eggs and oncomiracidia and theciliate Cryptocaryon irritans cysts and theronts [J]. Aquaculture, 2003, 223 (1-4): 1-13.) et al. used 2.4 mg / L sodium hypochlorite to kill Cryptocaryon irritans in vitro, killing 100 cells / mL of larvae after 1 minute; Li Meiying (LI Meiying. Preliminary investigation and prevention and control of parasitic diseases of farmed cold-water fish in Hebei Province [D]. Shijiazhuang: Hebei Normal University.) used 1 mg / L chlorine dioxide to bathe sturgeons (Acipenser) suffering from white spot disease. After 4 days, the survival rate of sturgeons was only 38.5%. These methods generally have the disadvantages of high killing doses, long processing time, harm to the healthy growth of farmed animals, residual chemicals in the drainage water will pose potential risks to the ocean, and manual spraying is inefficient. Therefore, it is crucial to automatically and efficiently kill parasites and diseases in farmed animals while ensuring the healthy growth of factory-farmed fish.

[0005] Hydroxyl radicals (·OH) generated efficiently by atmospheric pressure ionization discharge are strong oxidants with a 8 ~10 10 The reaction rate is 10 times that of other common oxidants. 6 ~10 12times, and can be converted into H2O and O2 after reacting with organic matter. And ·OH can cause irreversible reactions such as DNA structure breakage, protein oxidation and decomposition, lipid peroxidation, etc. in organisms, thus leading to the death of cells and organisms. The technology based on the efficient generation of hydroxyl free radicals by atmospheric pressure ionization discharge has been widely used in antibiotic mineralization (patent number: CN108658209A, CN108609715A), algae toxin mineralization (patent number: CN104341037A), odor substance mineralization (patent number: CN104402140A), high algae water treatment (patent number: CN104326529A), irritant Cryptocaryon cyst killing (patent number CN107027667A), irritant Cryptocaryon killing (patent number: CN107079852A). In view of the frequent occurrence of pathogenic infections such as parasites, bacteria, and viruses in factory-based marine aquaculture and the low efficiency of manual intervention, the strategic goal of reducing losses in farmed animals and green farming is to improve the ability of automated control of factory farming diseases and protect the safety of the marine ecological environment. The application of hydroxyl free radical technology to the source of disease input and transmission, automatic early warning and efficient prevention and control of aquaculture diseases, and ensuring the green and healthy operation of circular farming is of great significance to the green development of the marine aquaculture industry. Summary of the invention

[0006] In view of the problems in factory-based seawater aquaculture, such as the difficulty in controlling farmed animal diseases, low water purification efficiency, low intelligent control rate, and the inability to ensure the healthy growth of farmed fish and the safety of marine ecology during the killing of aquaculture diseases, the present invention provides a device and method for killing hydroxyl free radicals of parasites in factory-based seawater aquaculture, which provides a reliable technical basis for establishing an automated, efficient, green, safe and economical water treatment method for factory-based seawater aquaculture animal diseases and for the prevention and control of farmed animal diseases, and automatically circulates to complete the disinfection of water in seawater aquaculture ponds, purifies aquaculture water quality, and ensures the green development of the seawater aquaculture industry and the safety of marine ecology.

[0007] The device for killing hydroxyl radicals of parasites in factory-farmed animals comprises a hydroxyl radical (·OH) solution generating device, a ·OH solution microjet injection device, a mixing tank, a seawater culture pond, a microfilter / protein separator / biofilter, a sand filter-water reservoir, a real-time online detection system and an intelligent control center, which can achieve efficient killing of parasites in factory-farmed animals, no generation of disinfection by-products, purification of aquaculture water, and ensure the healthy growth of aquaculture animals.

[0008] The hydroxyl radical (·OH) solution generating device is connected to a mixing tank; the ·OH solution micro-jet injection device is installed at the bottom of a seawater aquaculture pond with a star-shaped injection pipe, and is connected to the mixing tank and the central pipe of the star-shaped micro-jet injection device through a ·OH solution delivery pipe, and the ·OH micro-jet injection kills the parasites of farmed animals; the microfilter / protein separator / biofilter is connected to the ·OH solution treatment aquaculture pond through a circulating external drainage pipeline; the sand filter-water reservoir provides water sources for the ·OH solution generating device and the seawater aquaculture pond through pipelines; the intelligent detection device is connected to each device, detector and detection feedback pipeline, and the intelligent control center receives detection information and outputs control information in a wired or wireless manner, controls the operating parameters of each valve, water pump, and ·OH solution generating device in the device, and regulates the automatic operation of the ·OH solution high-efficiency killing device system for factory-farmed animal parasites.

[0009] The OH solution generating device and the miscible tank are used in combination; the main body of the miscible tank is composed of a mixing tank body and a connected water level display tube; a pressure gauge and an exhaust pressure regulating valve are installed on the top of the mixing tank body; the exhaust valve is connected to a stainless steel ball needle valve, an exhaust gas digester, a pressure reducing valve and a flow meter in sequence; a water outlet and a drain valve are installed at the bottom; the ratio of the inner diameter to the height of the miscible tank is 0.2-0.3; the distance between the water inlet and the top is 5%-10% of the height of the miscible tank; the miscible volume in the tank is 1 / 3-2 / 3 of the total volume; the operating pressure in the tank is maintained at 0.05-0.20 MPa; and the tank is used in series or in parallel according to the treatment efficiency.

[0010] The OH solution is transported to the OH solution micro-jet injection device at the bottom of the culture pond through a pipeline. The micro-jet injection device consists of a star-shaped OH solution distribution pipe. The OH solution in the distribution pipe is uniformly provided by a central vertical pipe. The OH micro-jet injectors are evenly distributed on the distribution pipe, and the installation direction is parallel to the bottom of the pond. The contraction section of the OH solution micro-jet injector is fixed to the distribution pipe with a mounting thread, and the expansion section of the injector is arranged horizontally in a counterclockwise direction. The inlet and outlet of the micro-jet injector are both provided with contraction and expansion. The micro jet injector has a contraction section guide trough and a diffusion section guide trough; the circular slit at the throat is equipped with a negative pressure liquid suction port facing downward and 1 to 10 cm away from the bottom of the pool, so that the attached cysts and other diseases are sucked into the jet injector for full oxidation and killing; the micro jet injector has a throat diameter of 0.5 to 1 mm, a contraction angle of 12 to 15°, a throat length of 0.5 to 2 mm, an expansion angle of 20 to 30°, a diffusion section length of 1 to 6 mm, and a flow rate of 0.70 to 3.0 L / min.

[0011] The intelligent detection device is connected to various devices, detectors and detection feedback pipelines. The input information source for real-time detection includes the detection results of automated analysis instruments such as flow cytometers, multifunctional water quality detectors and TRO detectors, as well as the flow rates at various nodes of the system pipelines, the operating status of the water pumps and the water level information.

[0012] Based on the device system, the present invention provides a method for killing parasites of factory-farmed animals with OH. The method is that a portion of the seawater in the sand filter tank-water reservoir is transported to the OH solution treatment breeding pool through a water pipeline, and the other portion is efficiently generated into a high-concentration OH solution with oxygen active particles through a gas-liquid mixer and a mixing tank. The OH solution delivery pipe sprays the high-concentration OH solution and cavitation bubbles to the seawater breeding pool through a star-shaped OH solution micro-jet injection device, 100% killing the animal parasites and other pathogenic microorganisms in the breeding pool, and mineralizing the organic matter and oxidizing inorganic nitrogen in the breeding pool. The opening and closing of different OH solution water pipe valves are controlled to achieve synchronous or intermittent killing of parasites and pathogenic microorganisms in multiple breeding pools. The microfilter / protein separator / biofilter device treats and purifies the wastewater in the seawater breeding pool and reuses it in the water reservoir or discharges it. The method has no negative effects on the farmed animals and the marine environment in the seawater breeding pool, and is ecologically safe.

[0013] In the ·OH solution generating equipment, the oxygen active particle gas and sand filtered seawater are mixed by water jet cavitation in the gas / liquid mixer and then flow into the mixing tank. The ·OH solution and the oxygen active particle gas are further fully mixed in the mixing tank to increase the concentration of the ·OH solution and kill marine aquaculture diseases. The starting number of oxygen active particle generating sources in the ·OH solution generating equipment is 1 to 12 pieces, and the power is 200 to 3600W; the gas introduced is oxygen or air, and the air intake flow rate is 2.0 to 50L / min; the oxygen active fragment concentration of the gas in the gas / liquid mixer is 80 to 300mg / L, and the volume ratio of oxygen active fragments to water can be 1:10 to 100; the ·OH solution generating equipment produces a total oxidant concentration of 4 to 50mg / L, and the production volume is 10 to 20m 3 / h.

[0014] After the ·OH solution is produced by the equipment, it is sent to the seawater aquaculture pond to be treated by the ·OH solution delivery pipeline, which is equipped with a star-shaped micro-jet injection device. Each ·OH solution delivery pipe provides ·OH solution for two rows of parallel seawater aquaculture ponds, and a main valve is set at the input end, and a valve is set between each aquaculture pond and the ·OH solution delivery pipe. When the treatment system is running, the control center adjusts the number of simultaneous treatments of the seawater aquaculture ponds according to the equipment load.

[0015] Before the seawater micro-jet ejector of the same ·OH solution delivery pipe outputs ·OH solution, the water level in the seawater aquaculture pond is discharged to 1 / 2 to 2 / 3 of the total pond volume; the oxygen active particle solution of the ·OH solution generating device is efficiently generated into ·OH solution in the mixing tank and then transported to the ·OH solution ejection micro-jet device through the pipeline, wherein the pressure ratio between the inlet of the contraction section and the outlet of the diffusion section of the micro-jet ejector is 0.7 to 0.9; the high-concentration ·OH solution forms a large number of micro-bubbles through the diffusion section of the micro-jet ejector installed in the liquid distribution pipe, and the ·OH enters the aquaculture pond in the strong turbulent field and collapses to generate high temperature (10 4 K) and high pressure (10 4 MPa), quickly penetrates and diffuses into the surrounding liquid in the form of pressure micro-jet, 100% killing cysts attached to the bottom of the pond, the sprayed OH solution is fully miscible with the aquaculture water and the diffusion time is 2 to 30 minutes, and 100% of parasites and microorganisms are killed when the concentration is greater than 0.5 mg / L, and organic matter in the seawater aquaculture pond is mineralized and inorganic nitrogen is oxidized, achieving disinfection of the aquaculture pond while ensuring the health of the farmed fish.

[0016] The intelligent control center functions of the ·OH device system for killing parasites in factory-farmed animals include: dynamic control of ·OH solution concentration and output; dynamic control of ·OH solution concentration in seawater aquaculture ponds and parasite killing time of farmed fish; real-time fault diagnosis and prediction and early warning. The intelligent control center uses the dynamic detection results of seawater aquaculture pond water quality and the total oxidant concentration in the pipeline as input information, and dynamically controls the ·OH solution concentration (mg / L) and output (m / L) produced by the hydroxyl radical generating equipment based on the established prediction model of ·OH solution (concentration>0.5mg / L) killing parasites in the aquaculture pond and the ·OH concentration diffusion model during the killing process. 3 / h); dynamically detect the OH concentration and density of aquaculture pests in seawater aquaculture ponds; dynamically control the opening and closing of OH micro-jet tubes in different aquaculture ponds. Realize remote intelligent monitoring, data analysis, precise situation awareness, energy efficiency management and operation and maintenance decision-making of the entire system.

[0017] The water treatment related indicators of seawater aquaculture ponds meet the second category standards of the "Seawater Quality Standards" and the "Fishery Water Quality Standards" (GB 11607-89): sufficient dissolved oxygen in the water (>10mg / L), ammonia nitrogen below 0.5mg / L, nitrite nitrogen below 0.1mg / L, TOC below 4mg / L, and complete killing of harmful microorganisms such as aquaculture parasites, bacteria, viruses, etc., bromoform below 9.53μg / L (<100μg / L national standard), and no other disinfection by-products are detected.

[0018] The structure for water treatment is a microfilter / protein separator / biofilter device, which is composed of a microfilter, a protein separator and a biofilter device connected in series. A water distribution weir is installed inside the microfilter, and the aquaculture water enters the rotating drum through the guide pipe. A 60-100 mesh microporous filter is installed inside the drum to intercept the suspended matter in the aquaculture wastewater into the drum, and the guide blades inside the filter cartridge guide the suspended particles to the mud discharge trough at the other end; the protein separator uses the tiny bubbles released by the gas releaser to adsorb the soluble macromolecular organic matter in the water and float it to the water surface, and separate it through the protein collector on the liquid surface; the biological reaction tank contains a three-dimensional hollow filler as a microbial carrier, the filler is a hollow structure, aerobic bacteria grow outside, and anaerobic bacteria grow inside the filler.

[0019] The technical effects and advantages of the present invention are as follows:

[0020] 1. The present invention is based on a highly efficient OH solution generation device composed of a modular array plasma integrated source. Through water jet cavitation gas / liquid miscibility technology, a high-concentration oxygen free radical solution mainly composed of OH is obtained. The total oxidant concentration is as high as 50 mg / L, and the production amount is 10-20 m 3 The liquid / liquid miscible tank designed in the present invention provides miscible working parameter indicators, fully and evenly mixes the oxygen-containing active particles and the ·OH solution in the tank, efficiently generates the ·OH solution, and can be used in series / parallel mode according to the workload of the processing system.

[0021] 2. The star-shaped OH micro-jet injector for killing diseases of farmed animals in seawater aquaculture ponds provided by the present invention determines the parameters such as the inlet and outlet pressures, throat length, contraction angle and diffusion angle of the injector, so that a high concentration OH solution forms a large number of micro-bubbles at the outlet of the micro-jet injector, and then separates from the micro-jet injector in the strong turbulent field at the outlet and enters the factory-scale seawater aquaculture pond; the micro-bubbles will generate extremely high temperatures (10 4 K) and pressure (10 4MPa), the high-concentration ·OH solution will quickly penetrate and diffuse into the surrounding liquid in the form of a pressure microjet. After the ·OH solution jet breaks into "mist", it still has a high initial velocity. The ·OH is fully mixed and diffused in the seawater to keep its concentration above 0.5 mg / L. During the dynamic penetration and impact of ·OH, the diseases of the breeding pond and the wall of the breeding pond are efficiently killed and the healthy growth of the farmed animals is ensured. The liquid suction port arranged on the microjet injector can suck the cysts, parasites and other diseases attached to the bottom of the pool into the injector and mix them with the high-concentration ·OH solution and inject them into the breeding pond. This process kills the inhaled breeding diseases through the "explosion" of cavitation bubbles in the microjet injector and the continuous oxidation of ·OH. The star-shaped ·OH microjet injector designed by the present invention and the process advantages enable the ·OH solution to diffuse rapidly and efficiently kill insects in seawater breeding ponds with a volume of more than 60 tons, and the processing time is reduced to 2 to 30 minutes.

[0022] 3. The device system provided by the present invention is coordinated and operated through an intelligent control center. Based on the built-in multiple intelligent model algorithms, the concentration and output of the ·OH solution produced by the ·OH generating equipment are dynamically controlled; the concentration of the ·OH solution in the seawater aquaculture pond is dynamically detected and the parasite killing time of the farmed fish in the aquaculture pond is controlled. The multi-input, multi-output, multi-loop, multi-restriction and multi-objective optimization control problems of ·OH solution generation and aquaculture pond disease killing are solved. According to the actual seawater quality, water consumption, aquaculture disease outbreak time, and active oxygen free radical solution generation parameters, the input and output system has effectively solved the problem of lag, and can perform fault diagnosis and prediction and early warning in real time, realizing remote intelligent monitoring, data analysis, situation awareness, energy efficiency management and operation and maintenance decision-making of the entire system. The system indication accuracy is less than 0.1%, and the control response time is less than 0.1 seconds.

[0023] 4. When the device system provided by the present invention is in operation, the aquaculture diseases in different seawater aquaculture ponds can be killed separately or simultaneously, and the killing time can be controlled according to the real-time aquaculture pond seawater quality, so that the system process can operate efficiently. The system innovatively combines the ·OH advanced oxidation technology and the factory-scale circulating aquaculture system. After the ·OH solution sterilizes and disinfects the aquaculture water in the aquaculture pond, the aquaculture water quality of the aquaculture pond meets the second category standard of the "Seawater Quality Standard" and the "Fishery Water Quality Standard" (GB 11607-89) standard: the water body has sufficient dissolved oxygen (>10mg / L), ammonia nitrogen is less than 0.5mg / L, nitrite nitrogen is less than 0.1mg / L, TOC is less than 4mg / L, and harmful microorganisms such as aquaculture parasites, bacteria, and viruses are completely killed, tribromoform is less than 9.53μg / L (<100μg / L national standard), other disinfection by-products are not detected, and the residual oxidant TRO concentration is less than 0.3mg / L, which can achieve continuous sterilization and disinfection. Aquaculture wastewater without disease and organic pollution can be reused as aquaculture raw water, OH solution water or directly discharged after being treated by the integrated device of microfiltration machine / protein separator / biofilter. It has no negative effect on the growth and health of farmed animals and marine ecology, and is ecologically safe.

[0024] The invention is simple to implement, highly efficient and has no negative effect on the environment, and provides a new idea and feasible technical means for industrial seawater circulation aquaculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 .Schematic diagram of the system for the efficient killing of parasites in factory-farmed fish by hydroxyl radicals

[0026] Figure 2 .Flowchart of how hydroxyl free radicals can effectively kill parasites in factory-farmed fish

[0027] Figure 3 Schematic diagram of the “star-shaped” microjet injection of OH solution in the aquaculture pond

[0028] Figure 4 Liquid / liquid mixing tank and micro jet ejector structure diagram

[0029] Figure 5 Schematic diagram of microfilter / protein separator / biofilter device

[0030] Figure 6 Bright field, DAPI staining, and HE staining of OH killing cysts

[0031] Figure 7 ·OH concentration distribution diagram of the aquaculture pond disease treated by ·OH solution miscible treatment: (a) ·OH concentration distribution diagram of the aquaculture pond after injection; (b) ·OH solution concentration distribution diagram of the aquaculture pond after 5 minutes of stabilization

[0032] Figure 8HE staining picture of large yellow croaker intestine

[0033] In the figure: 11-first valve; 12-second valve; 13-third valve; 14-fourth valve; 15-fifth valve; 16-sixth valve; 17-seventh valve; 18-eighth valve; 19-ninth valve; 110-tenth valve; 111-eleventh valve; 112: twelfth valve; 113-thirteenth valve; 114-fourteenth valve; 21-first water pump; 22-second water pump; 23-third water pump; 31-first flow meter; 32-second flow meter; 33-third flow meter ;41-sand filter;42-water reservoir;43-microfilter / protein separator / biofilter device;431-microfilter;432-protein separator;433-biofilter device;434-water distribution weir;435-flow guide pipe;436-rotating drum;437-mud discharge trough;438-gas releaser;439-protein collector;4310-three-dimensional hollow filler;51-gas-liquid mixer;52-mixing tank;521-mixing tank;522-connecting water level display tube;523-pressure regulating valve ; 524-pressure gauge; 525-stainless steel needle valve; 526-exhaust digester; 527-pressure reducing valve; 528-flow meter; 53-active oxygen particle gas generator; 6-hydroxyl radical (·OH) generating equipment; 61-active oxygen particle gas concentration detector; 62-first TRO concentration detector; 63-flow cytometer; 64-multifunctional water quality detector; 65-second TRO detector; 7-intelligent control center; 71-sand filter seawater pipeline; 72-·OH solution delivery pipe ; 73-circulation external drainage pipeline; 74-detection feedback pipeline; 75-oxygen free radical preparation pipeline; 8-intelligent detection device; 81-·OH solution treatment seawater aquaculture pond; 82-microjet injection device; 83-·OH microjet injector; 831-injector mounting thread; 832-injector contraction section; 833-annular slit; 834-injector throat section; 835-injector expansion section; 836-injector negative pressure liquid suction port; 837-contraction section guide groove; 838-diffusion section guide groove.

[0034] The structure for water treatment is a microfilter / protein separator / biofilter device, which is composed of a microfilter, a protein separator and a biofilter device in series. A water distribution weir is installed inside the microfilter, and the aquaculture water enters the rotating drum through the guide pipe. A 60-100 mesh microporous filter is installed in the drum to intercept the suspended matter in the aquaculture wastewater into the drum. The guide blades inside the filter cartridge guide the suspended particles to the mud discharge trough at the other end; the protein separator uses the tiny bubbles released by the gas releaser to adsorb the soluble macromolecular organic matter in the water and float it to the water surface, and separate it through the protein collector on the liquid surface; the biological reaction tank contains a three-dimensional hollow filler as a microbial carrier, the filler is a hollow structure, aerobic bacteria grow outside, and anaerobic bacteria grow inside the filler. DETAILED DESCRIPTION

[0035] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings. However, the protection scope of the present invention is not limited to the embodiments.

[0036] like Figures 1 to 5 As shown, a set of hydroxyl radical killing device system for factory-farmed animal parasites is built, including hydroxyl radical (·OH) solution generating equipment 6, ·OH solution micro-jet injection device 82, mixing tank 52, seawater aquaculture pond 81, microfilter / protein separator / biofilter 43, sand filter 41-water reservoir 42, real-time online detection system 8 and intelligent control center 7, which can achieve efficient killing of factory-farmed animal parasites, no generation of disinfection by-products, purification of aquaculture water, and ensure the healthy growth of aquaculture animals.

[0037] The water source of the inlet of a factory-farmed animal parasite hydroxyl radical killing device system is taken from seawater 0. The seawater inlet pipeline is connected to the sand filter 41 through the first valve 11, the first water pump 21 and the first flowmeter 31. The sand filter 41 and the reservoir 42 are connected through a water pipe provided with a second valve 12. A pipeline of the reservoir 42 is connected to the ·OH solution generating device 6 through the third valve 13. In the ·OH solution generating device 6, oxygen or air is connected to the oxygen active particle gas generating device 53 through the oxygen free radical preparation pipeline 75 through the thirteenth valve 113, and is passed into the gas / liquid mixer 51 after being detected by the oxygen active particle concentration detector 61. The material of the oxygen free radical preparation pipeline 75 is a polytetrafluoroethylene tube. The water inlet pipeline of the gas / liquid mixer 51 is provided with a second water pump 22 and a third flowmeter 33, and the water outlet pipeline is connected to the mixing tank 52. A first TRO concentration detector 62 is provided on the liquid outlet pipeline of the miscible tank 52, and the opening and closing of different OH solution delivery pipes 72 are controlled by the fifth valve 15. Branch pipes connected to the star-shaped OH solution micro-jet injection device 82 in the seawater aquaculture pond 81 are distributed on the OH solution delivery pipe 72, which is controlled by the sixth valve 16.

[0038] The miscible tank 52 in the OH solution generating device 6 is mainly composed of a mixing tank body 521 and a connected water level display tube 522. A pressure gauge 524 and an exhaust pressure regulating valve 523 are installed on the top of the mixing tank body 521. The exhaust valve is connected to a stainless steel ball needle valve 525, an exhaust gas digester 526, a pressure reducing valve 527 and a flow meter 528 in sequence. A water inlet and a drain valve are installed at the bottom. The ratio of the inner diameter and height of the miscible tank is 0.25. The distance from the water inlet to the top is 8% of the height of the miscible tank. The miscible volume in the tank is 1 / 3 to 2 / 3 of the total volume. The operating pressure in the tank is maintained at 0.05 to 0.20 MPa. The operating parameters are adjusted in real time by the intelligent control center 7 according to the processing efficiency.

[0039] The micro jet injection device 82 is composed of a star-shaped ·OH solution distribution pipe. The ·OH solution in the distribution pipe is uniformly provided by a central vertical pipe. ·OH micro jet injectors 83 are distributed on the distribution pipe at intervals of 40 cm. The installation direction is parallel to the bottom of the pool. A total of 48 micro jet injectors are installed. DN50 is used as the main pipeline, and the star-shaped water distribution pipe uses DN25 PVC as the branch pipeline. The contraction section 832 of the ·OH solution micro jet injector 83 is fixed to the distribution pipe with an installation thread 831, and the expansion section 835 of the injector is arranged horizontally in a counterclockwise direction. The circular slit 833 at the throat 834 is arranged with a negative pressure liquid suction port 836 facing downward and 2 cm away from the bottom of the pool. The inlet and outlet of the micro jet injector are both provided with a contraction section guide groove 837 and a diffusion section guide groove 838. The throat diameter of the microjet ejector is 0.75 mm, the contraction angle is 15°, the throat length is 1 mm, the expansion angle is 25°, the diffusion section length is 4 mm, and the flow rate of the microjet ejector is 2.5 L / min. ·OH microjet ejector contraction section pressure (P in ) and the outlet pressure of the diffuser (P out ) of the pressure difference ratio (P r =(P in -P out ) / P in ) should be 0.9.

[0040] Another outlet pipeline of the water storage tank 42 is the sand filter seawater pipeline 71, on which the fourth valve 14 and the second flow meter 32 are arranged. The sand filter seawater pipeline 71 is connected to the branch pipes entering each seawater aquaculture pool, which is controlled by the fourteenth valve 114. The wastewater after the OH treatment of the seawater aquaculture pool 81 is collected to the microfilter / protein separator / biological filter device 43 through the seventh valve 17 and the circulating external drainage pipeline 73. The water inlet pipeline 73 of the treatment device 43 is provided with a main valve 18. The outlet pipeline of the microfilter / protein separator / biological filter device 43 is connected to the seaside through the ninth valve 19 for external discharge or connected to the water storage tank 42 for reuse through the tenth valve 110.

[0041] In the device system, the seawater aquaculture pool 81 and the sand filter seawater pipeline 71 are connected to the flow cytometer 63, the multifunctional water quality detector 64, and the second TRO detector 65 in the intelligent detection device 8 through the detection feedback pipeline 74, and are controlled by the eleventh valve 111, the twelfth valve 112 and the third water pump 23. The signal input source of the intelligent control center 7 in the device system includes the intelligent detection device 8, the active oxygen particle gas concentration detector 61, the first TRO concentration detector 62 and other flow meters and water pumps, and the signal output targets include various valves and water pumps. The input and output of the signal are performed in a wired or wireless manner.

[0042] When the device system is in operation, the sand filter tank 41-water reservoir 42 filters the suspended solid particles in the nearshore seawater and stores the sand-filtered seawater. Most of the sand-filtered seawater is input into the seawater aquaculture pond 81 as a breeding water source, and part of it is input into the ·OH solution generating device 6 to produce a high-concentration ·OH solution. The number of array-type oxygen active particle sources that can be started in the hydroxyl radical (·OH) solution generating device is 1 to 12, the working power is 200 to 3600W, the gas introduced is oxygen, and the air intake flow rate can be 25L / min; the gas pipeline adopts polytetrafluoroethylene tube or other antioxidant materials; according to the active oxygen particle gas concentration detector 61, the concentration is 80 to 300mg / L, and the components of the oxygen active fragments mainly include O2 + 、O2 - 、O( 3 P), O( 1 D), O2(a 1 g) and O3, among which O2 + It is the key active particle for generating ·OH. The volume ratio of oxygen active fragment input to sand filtered seawater input in gas / liquid mixer 51 can be 1:(10-100), and the actual ratio is determined by TRO concentration and killing effect fed back by intelligent control center 7 in real time. After fully mixing in mixing tank 52, the total oxidant concentration outputted from ·OH solution generating device 6 is as high as 50mg / L, and the generated amount is between 10 and 20m 3 / h, ·OH concentration is 50~600μmol / L.

[0043] The ·OH solution water pipe 72 transports the ·OH solution produced by the ·OH solution production device 6 to the mixing tank 52, and transports the mixed and stable ·OH solution in the mixing tank 52 to the star-shaped ·OH solution micro-jet injection device 82 in the seawater aquaculture pond 81, so as to achieve the ·OH solution to dynamically kill aquaculture diseases and purify the aquaculture water quality. The ·OH solution micro-jet injection device 82 installed at the bottom of the seawater aquaculture pond transports the ·OH solution and cavitation bubbles to the aquaculture pond, sprays the aquaculture pond in all directions, and fully mixes the high-concentration ·OH solution with the original aquaculture water to a total oxidant TRO concentration greater than 0.5 mg / L. The cysts attached to the bottom of the pond are effectively killed by the high-concentration ·OH solution in the micro-jet injector 83 and the rupture of the outlet microbubbles. The ·OH solution entering the aquaculture pond can kill the parasites of the aquaculture fish in the aquaculture pond, mineralize the organic matter in the aquaculture pond, oxidize inorganic nitrogen, and sterilize and disinfect the aquaculture water. After the OH miscible treatment in the aquaculture pond, the concentrations of ammonia nitrogen, nitrite nitrogen and TOC are reduced, and the harmful microorganisms such as aquaculture parasites, bacteria and viruses can be completely killed, and the aquaculture diseases in the aquaculture pond can be finally treated. After treatment, the water quality indicators can meet the "Fishery Water Quality Standard" (GB 11607-89).

[0044] The wastewater discharged from the seawater aquaculture pond is transported to the microfilter / protein separator / biofilter device 43 for treatment through the circulating external drainage pipeline 73, and the treated tail water is discharged to the sea or the reservoir 42. The microfilter / protein separator / biofilter device 43 treats the wastewater discharged from the ·OH solution-treated aquaculture pond by physical biological treatment to remove pollutants such as suspended particles and proteins and purify the water quality. The microfilter / protein separator / biofilter device 43 is composed of a microfilter 431, a protein separator 432 and a biofilter device 433 connected in series. A water distribution weir 434 is installed inside the microfilter. The aquaculture wastewater enters the rotating drum 436 through the guide pipe 435. A 100-mesh microporous filter is installed in the drum to intercept the suspended matter in the aquaculture water into the drum. The guide blades inside the filter cartridge guide the suspended particles to the mud discharge trough 437 at the other end to separate the particles in the aquaculture water and effectively remove 20% to 50% of the turbidity. The protein separator uses tiny bubbles released by the gas releaser 438 to form bubbles on the surface of food residues and proteins. The bubble size is 50 to 100 μm, which adsorbs soluble macromolecular organic matter in the water and floats it to the water surface. The protein is separated by a protein collector 439 on the liquid surface, which significantly increases the dissolved oxygen in the water, removes 80% of the metabolites of farmed fish, and reduces 15-25% of ammonia nitrogen and 10-20% of nitrite nitrogen. The biological reaction tank contains a three-dimensional hollow filler 4310 as a microbial carrier. The filler has a hollow structure, and aerobic bacteria grow on the outside to remove organic matter and convert ammonia nitrogen into nitrate nitrogen through nitrification. Anaerobic bacteria grow inside the filler to produce denitrification to remove nitrate nitrogen. The aerobic and anaerobic processes are combined to further remove 40%-50% of ammonia nitrogen, 5%-15% of nitrite nitrogen and 10%-20% of TOC.

[0045] The detection feedback pipeline 74 draws water samples from the ·OH solution treated aquaculture pond 81 and the sand filtered seawater water pipe 71 to the intelligent detection device 8. The built-in flow cytometer 63, multifunctional water quality detector 64 and TRO detector 65 of the intelligent detection device 8 monitor the aquaculture diseases, water quality and ·OH solution concentration in the aquaculture pond 81 treated with ·OH in real time, and feeds back the detection information to the intelligent control center 7. The multifunctional water quality detector is equipped with temperature (-5~50℃), salinity (0~70ppt), turbidity (0~400NTU), dissolved oxygen (0~50mg / L), ammonia nitrogen (0~20mg / L), nitrite nitrogen (0~20mg / L), fDOM dissolved organic matter (0~300ppb), etc. The TRO detector is based on the N,N-diethyl-p-phenylenediamine (DPD) spectrophotometric detection (in terms of Cl) of the US EPA standard. The intelligent control center 7 has multiple built-in intelligent model algorithms: dynamic control of the concentration and output of OH solution produced by hydroxyl radical generating equipment; dynamic detection and processing of OH solution concentration in the breeding pond to control the parasite killing time of farmed fish in the breeding pond; dynamic control of the start of killing of OH micro-jet injectors in different breeding ponds; real-time fault diagnosis and prediction and early warning to realize remote intelligent monitoring, data analysis, situation awareness, energy efficiency management and operation and maintenance decision-making of the entire system.

[0046] The following is a specific embodiment of a device and method for killing parasites in factory-farmed animals:

[0047] Example 1

[0048] Depend on Figure 2 As shown, the operation process of the factory-farmed animal parasite hydroxyl radical killing device is as follows:

[0049] 1) Open the first valve 11 and start the first water pump 21. The coastal seawater is transported to the sand filter tank 41 through the sand filter seawater water pipe 71 and then stored in the water reservoir 42 after being filtered.

[0050] 2) The sand-filtered seawater enters the OH solution treatment breeding pond 81 through the fourth valve 14, the second flowmeter 32 and the 14th valve to breed marine animals.

[0051] 3) When the seawater aquaculture pond 81 needs to use ·OH solution to kill parasites and diseases in the aquaculture pond, the seventh valve 17 is opened to discharge the seawater in the aquaculture pond 81 to be treated with ·OH solution to 1 / 2 to 2 / 3 of the total pond volume.

[0052] 4) Open the eleventh valve 111, start the third water pump 23, and the seawater in the sand-filtered seawater water pipe 71 enters the intelligent detection device 8 through the detection feedback pipeline 74. The built-in flow cytometer 63 and water quality detector 64 of the intelligent detection device 8 are used to detect the disease conditions and water quality conditions of the sand-filtered seawater aquaculture, and the results are fed back to the intelligent control center 7. Close the eleventh valve 111, open the twelfth valve 112, start the third water pump 23, and the seawater in the ·OH solution-treated aquaculture pool 81 enters the intelligent detection device 8 through the detection feedback pipeline 74. The built-in flow cytometer 63 and water quality detector 64 of the intelligent detection device 8 are used to detect the water quality and aquaculture disease conditions of the aquaculture pool, and the results are fed back to the intelligent control center 7.

[0053] 5) The intelligent control center 7 controls the oxygen to enter the high concentration hydroxyl radical (·OH) solution generating device 6 through the oxygen free radical preparation pipeline 75 according to the feedback information, and dissociates the oxygen to generate a high concentration of oxygen active particle gas through strong ionization discharge. After the concentration is detected by the oxygen active particle concentration detector 61, the gas is fed back to the intelligent control center 7.

[0054] 6) The high concentration hydroxyl radical (·OH) solution produced by the high concentration hydroxyl radical (·OH) solution production device 6 is mixed evenly in the liquid / liquid miscibility device 51 through turbulence and cavitation effects to produce a large yield of ·OH solution and an appropriate ·OH solution concentration. The solution passes through the miscibility tank 52, and the unmixed oxygen active particles are further fully mixed to achieve full mixing of the ·OH solution and stability of the ·OH solution concentration. The number of micro jet injectors to be opened (1 to 3) is determined based on the TRO concentration of the ·OH solution to be treated in the aquaculture pond, and is monitored in real time through the TRO concentration detector 62 and fed back to the intelligent control center 7.

[0055] 7) The intelligent control center 7 delivers the high-concentration OH solution to the ·OH solution treatment breeding pool 81 through the high-concentration ·OH solution delivery pipeline 72 according to the treatment time of a single ·OH solution treatment breeding pool, opens the sixth valve 16, and transmits the ·OH solution to the ·OH solution treatment breeding pool 81 through the pre-installed breeding pool micro-jet device 82 to the ·OH micro-jet injector 83.

[0056] 8) A total of 48 microjet ejectors 83 are installed on the microjet ejector device 82 of the aquaculture pond. The pressurized ·OH solution flows through the microjet ejector 83, and a large number of cavitation microbubbles are generated at the throat thereof after diversion. The cavitation microbubbles cavitate and expand in the decompression diffusion section, and are ejected from the microjet ejector 83 by the water flow. The ·OH solution entrained with the cavitation microbubbles contacts the planktonic Cryptocaryon and Vibrio in the aquaculture pond, and the cavitation microbubbles collapse instantly around them to generate high temperature and high pressure, and the ·OH solution can kill the aquaculture diseases at a rapid speed.

[0057] 9) During the killing process of the breeding pond by the microfluidic device 82 in the breeding pond, the twelfth valve 112 is opened, the eleventh valve 111 is closed, and the third water pump 23 is started. The seawater in the breeding pond 81 treated with ·OH solution enters the intelligent detection device 8 through the detection feedback pipeline 74. The built-in flow cytometer 63, multifunctional water quality detector 64 and TRO detector 65 of the intelligent detection device 8 are used to monitor the breeding diseases, water quality and concentration of ·OH solution in the breeding pond treated with ·OH in real time, and the detection information is fed back to the intelligent control center 7 to optimize the preparation plan of ·OH solution and adjust the operation time. After the TRO detector 65 detects that the concentration of ·OH solution in the breeding pond 81 treated with ·OH solution is diffused to 0.5-1.0 mg / L, the intelligent control center 7 stops the treatment of the breeding pond 81 treated with ·OH solution according to the feedback information.

[0058] 10) The intelligent control center 7 can dynamically control the number of breeding ponds treated with ·OH solution, water quality, and breeding disease conditions according to actual needs until all breeding ponds treated with ·OH solution are completed.

[0059] 11) OH solution treatment The aquaculture water discharged from the aquaculture pond 81 is pre-treated by the microfilter / protein separator / biofilter device 43, and the treated clean water is stored in the reservoir through the circulating external drainage pipeline 73. The excess wastewater is discharged into the sea after being tested and found to meet the standards.

[0060] In this embodiment, the concentration and yield of oxygen free radicals are controlled by the number of oxygen active fragment generation source modules in parallel, gas discharge power, oxygen intake volume and other parameters; the concentration of oxygen free radicals is controlled by the concentration of oxygen active fragments, gas-liquid volume ratio, equilibrium partial pressure of oxygen-containing active fragment gas and water temperature; the concentration of oxygen free radicals is controlled by the COD Cr It is determined by the water quality conditions such as concentration, turbidity, ammonia nitrogen, nitrite nitrogen, TOC, etc.

[0061] Example 2

[0062] The experiment of killing Cryptocaryon irritans in circulating water with ·OH was conducted in a farm in Ningde, with seawater temperature of 27.2℃, salinity of 32, and pH=8.01. The culture pond is a cylinder with a diameter of 6.1 meters and a depth of 2.1 meters, and a micro-jet device is installed 0.1 meters from the bottom. The diameter of the main water distribution pipe of the micro-jet device is DN50, and the diameter of 8 star-shaped water distribution pipes with a length of 2.8 meters is DN25 and is spaced 45° between each other. The star-shaped water distribution pipes are arranged at intervals of 40 cm. A total of 48 micro-jet injectors are installed, and each water distribution pipe is equipped with 6 micro-jet injectors. High-concentration ·OH solution is sprayed into the culture pond through the ·OH micro-jet injector to dissolve and kill Cryptocaryon irritans in the culture pond, and compared with conventional chemical agents ClO2 and formaldehyde, the killing effect of ·OH and ClO2 / HCHO on Cryptocaryon irritans larvae and microorganisms in seawater is studied, and the experiment is repeated three times. The experiment used a cylindrical sampling bag made of a sieve to collect larvae. The sampling bag was hung in the breeding pond. After 5 mL of larval liquid with a concentration of 10,000 cells / mL was injected into the sampling bag, the concentration of the liquid in the sampling bag was 1,000 cells / mL. The sampling bag was taken out after the reaction time was over. The larvae in the sampling bag were collected, and the FDA / PI fluorescent staining method was used to determine the life and death of the larvae. The size, morphology, and vitality of the larvae were detected under an optical microscope (Leica, DM3000). The experimental results are shown in Table 1.

[0063] Table 1. Comparison of OH / ClO2 / HCHO insecticides in breeding ponds

[0064]

[0065] According to Table 1, ·OH with TRO = 0.50 mg / L can completely kill Cryptocaryon irritans with a concentration of 1000 cells / mL in 6 seconds, and the larval killing rate is 100%. However, 1.0 mg / L ClO2 needs 10 minutes to completely kill the larvae; 100 mg / L formaldehyde needs 5 minutes to completely kill the larvae. Compared with conventional chemical agents ClO2 and formaldehyde, the ·OH kills the larvae at a dose of 0.5 mg / L and the time is only 6 seconds. This is because ·OH is a strong oxidant with strong oxidizing properties and fast reaction rate. It can quickly and efficiently kill Cryptocaryon irritans larvae; while ClO2 and formaldehyde require higher doses and longer time to kill the larvae.

[0066] The killing effect of ·OH and ClO2 / HCHO on microorganisms in seawater was compared. After 6s of treatment with TRO = 0.50mg / L ·OH, no pathogenic microorganisms in the aquaculture water were detected, and the killing rate was 100%; after 20min of treatment with 1.0mg / L ClO2 and 100mg / L formaldehyde, the killing rate of pathogenic microorganisms in the aquaculture water was 100%. Although no microorganisms were detected in seawater after treatment with ·OH / ClO2 / HCHO, compared with ClO2 and formaldehyde, ·OH has a low dose (0.50mg / L) and a short time (6s), while ClO2 and formaldehyde require higher doses and longer times to achieve the same effect.

[0067] Example 3

[0068] The above combined system was used in a farm in Sandu’ao, Ningde, to kill Cryptocaryon cysts attached to the ponds of large yellow croaker using hydroxyl radical solution. 12 pieces of oxygen active particle generators, O2 intake volume of 1.5m 3 / h, the discharge power is adjusted to 2.5KW, the concentration of oxygen active fragments produced by the OH generator is 186.8mg / L; the water volume is 8m 3 / h, the concentration of oxygen free radical solution entering the culture pond is 18.02mg / L, and the cysts adhering to the culture pond are flushed for 5 minutes by the ·OH microfluidizer, and the lethal threshold is 90. The cysts on the culture pond are sampled, and the bright field, DAPI staining, and HE staining of the cysts in the culture pond are shown in the figure below. Figure 6 shown.

[0069] Example 4

[0070] The aquaculture circulating water experiment was carried out in a farm in Ningde. According to the water quality to be treated, the OH generator turned on 4 oxygen active particle sources, adjusted the discharge power to 0.4KW, and the O2 intake volume to 0.6m 3 / h, so that the concentration of oxygen active fragments produced by the ·OH generating device is 87.6mg / L. By adjusting the gas-liquid ratio and the ·OH solution / water solution ratio, the concentration of the ·OH solution after liquid-liquid miscibility is controlled to be 4.15±0.02mg / L. 3 / h flow rate of high concentration ·OH solution is sprayed into the culture pond through the ·OH micro-jet injector. Figure 7 The figure shows the distribution diagram of ·OH concentration in the treatment of aquaculture pond diseases by mixing ·OH solution. The solution is evenly mixed in the aquaculture pond and achieves the purpose of water purification and sterilization.

[0071] The changes in water quality before and after treatment are shown in Table 2. After treatment, water quality indicators such as ammonia nitrogen and nitrite nitrogen all reached the fishery water quality standards; the total number of colonies, number of Vibrio, total coliform group, and fecal coliform group in sand filtered seawater were 3.5×104 CFU / mL, 1.8×10 2 CFU / mL, 5.6×10 2 MPN / 100mL and 73MPN / 100mL can cause bacterial infection in yellow croaker, resulting in mouth grinding, body surface ulcers, fin rot and other phenomena; the total bacteria, Vibrio, total Escherichia coli and heat-resistant Escherichia coli in the breeding pond after treatment with OH solution were not detected; the disinfection by-products bromate, chloroform, dibromochloromethane and dichlorobromomethane were not detected; the amount of bromoform generated was only 9.53μg / L, lower than 100μg / L (National Drinking Water Quality Standard), and there is no potential risk to the marine environment.

[0072] In summary, after being treated in the aquaculture pond with high-concentration ·OH solution, the water quality indicators such as dissolved oxygen (156.8%), ammonia nitrogen (40%), and turbidity (37.1%) in the seawater can be significantly improved, and the fecal coliform bacteria, total colony count, Vibrio count, and E. coli count in the water body were not detected. The water quality indicators, microbial indicators, and disinfection by-product indicators all meet the standards of the "National Drinking Water Quality Standard" (GB5749-2006), and the water quality meets the second category standard requirements of the "Seawater Quality Standard" to ensure the safety of the marine ecology. The large yellow croaker cultured in water treated with ·OH solution has an intact intestinal tissue structure, long and large in number intestinal villi, and neatly arranged intestinal epithelial cells, which will not cause damage to the intestinal tissue structure. Figure 8 shown.

[0073] Table 2 Water quality of factory aquaculture·OH-treated aquaculture seawater

[0074]

[0075] Note: ND means not detected.

[0076] The technical solutions disclosed and proposed by the present invention can be realized by those skilled in the art by referring to the contents of this article and appropriately changing the conditions, routes and other links. Although the methods and preparation techniques of the present invention have been described through preferred embodiments, relevant technicians can obviously modify or re-combine the methods and technical routes described herein without departing from the content, spirit and scope of the present invention to realize the final preparation technology. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the spirit, scope and content of the present invention.

Claims

1. A device for killing parasites in factory-farmed animals, characterized in that: It includes hydroxyl radical (•OH) solution generating equipment, •OH solution micro jet injection device, mixing tank, seawater aquaculture pond, microfilter / protein separator / biofilter, sand filter-water reservoir, real-time online detection system and intelligent control center, which can achieve efficient killing of parasites in factory-farmed animals, no generation of disinfection by-products, purification of aquaculture water, and ensure the healthy growth of aquaculture animals; The •OH solution generating device is connected to the mixing tank; the •OH solution micro-jet injection device is installed at the bottom of the seawater aquaculture pond with a star-shaped injection pipe, and is connected to the star-shaped central pipe through the •OH solution delivery pipe, and the •OH micro-jet injection kills the parasites of the farmed animals; the microfilter / protein separator / biofilter is connected to the •OH solution treatment aquaculture pond through a circulating external drainage pipeline; the sand filter-water reservoir provides water sources for the •OH solution generating device and the seawater aquaculture pond respectively through pipelines; the intelligent detection device is connected to each device, detector and detection feedback pipeline, and the intelligent control center receives detection information and outputs control information by wired or wireless means to regulate the automatic operation of the •OH solution high-efficiency killing of factory-farmed animal parasites device system; •OH solution is transported to the •OH solution micro-jet injection device at the bottom of the culture pond through a pipeline. The device is composed of star-shaped •OH solution micro-jet injection pipes. •OH micro-injectors are evenly distributed on the micro-jet injection pipes, and their installation direction is parallel to the bottom of the pond; Part of the seawater in the sand filter tank-reservoir is transported to the •OH solution treatment aquaculture pond through the water pipeline, and the other part is efficiently generated with oxygen active particles through the gas-liquid mixer and the mixing tank to generate •OH solution with a concentration of 4~50 mg / L. The •OH solution delivery pipe sprays the •OH solution and cavitation bubbles into the seawater aquaculture pond through the star-shaped •OH solution micro-jet injection device, which 100% kills the animal parasites and other pathogenic microorganisms in the aquaculture pond, mineralizes the organic matter in the aquaculture pond, and oxidizes the inorganic nitrogen; the opening and closing of the valves of different •OH solution water pipes are controlled to achieve synchronous or intermittent killing of parasites and pathogenic microorganisms in multiple aquaculture ponds; the microfilter / protein separator / biofilter device treats and purifies the wastewater in the seawater aquaculture pond and reuses it in the water reservoir or discharges it externally.

2. The device for killing parasites in factory-farmed animals as claimed in claim 1, characterized in that: •The OH solution generating equipment and the mixing tank are used together; the main body of the mixing tank is composed of a mixing tank body and a connected water level display tube. A pressure gauge and an exhaust valve are installed on the top of the mixing tank. The exhaust valve is connected to a stainless steel ball valve, an exhaust gas digester, a pressure reducing valve and a flow meter in sequence. A water outlet and a drain valve are installed on the bottom; the ratio of the inner diameter and height of the mixing tank is 0.2~0.3, the water inlet is 5%~10% of the height of the mixing tank from the top, the mixed volume in the tank should be 1 / 3~2 / 3 of the total volume, and the pressure in the tank is 0.05~0.20 MPa. It is used in series or parallel according to the treatment efficiency.

3. The device for killing parasites in factory-farmed animals as claimed in claim 1, characterized in that: •The contraction end of the OH micro-injector is fixed to the water distribution pipe with a thread, the diffusion section is arranged counterclockwise, the liquid suction port is downward and 1~10 cm away from the bottom of the pool; the diameter d of the micro-injector throat is 0.5~1 mm, the expansion angle is 20~30°, the contraction angle is 12~15°, the throat length is 0.5~2 mm, the diffusion section length is 1~6 mm, and the flow rate of the micro-injector is 0.70~3.0 L / min.

4. A method for killing parasites in factory-farmed animals using the device according to claim 1, characterized in that: Part of the seawater in the sand filter tank-reservoir is transported to the •OH solution treatment aquaculture pond through the water pipeline, and the other part is efficiently generated with oxygen active particles through the gas-liquid mixer and the mixing tank to generate •OH solution with a concentration of 4~50 mg / L. The •OH solution delivery pipe sprays the •OH solution and cavitation bubbles into the seawater aquaculture pond through the star-shaped •OH solution micro-jet injection device, which 100% kills the animal parasites and other pathogenic microorganisms in the aquaculture pond, mineralizes the organic matter in the aquaculture pond, and oxidizes the inorganic nitrogen; the opening and closing of the valves of different •OH solution water pipes are controlled to achieve synchronous or intermittent killing of parasites and pathogenic microorganisms in multiple aquaculture ponds; the microfilter / protein separator / biofilter device treats and purifies the wastewater in the seawater aquaculture pond and reuses it in the water reservoir or discharges it externally.

5. The method for killing parasites in factory-farmed animals as claimed in claim 4, characterized in that: In the •OH solution generating device, the oxygen active particle gas and the sand filtered seawater are mixed by water jet cavitation in the gas / liquid mixer and then flow into the mixing tank. The •OH solution and the oxygen active particle gas are fully mixed in the mixing tank to increase the concentration of the •OH solution and simultaneously kill marine aquaculture diseases. The number of oxygen active particle generating sources in the •OH solution generating device is 1 to 12, and the power is 200 to 3600 W. The gas introduced is oxygen or air, and the air intake flow rate is 2.0 to 50 L / min. The oxygen active fragment concentration of the gas in the gas-liquid mixer is 80 to 300 mg / L, and the volume ratio of oxygen active fragments to water is 1:10 to 100. The •OH solution generating device generates a total oxidant concentration of 4 to 50 mg / L and a production volume of 10 to 20 m 3 / h.

6. The method for killing parasites in factory-farmed animals as claimed in claim 4, characterized in that: Before the microjet tube sprays the •OH solution, the water level in the breeding pond is drained to 1 / 2~2 / 3 of the total pond volume; the oxygen active particle solution of the •OH solution generating equipment is efficiently converted into •OH solution in the mixing tank and then transported to the •OH solution spraying microjet device through a pipeline, wherein the pressure ratio between the inlet of the contraction section and the outlet of the diffusion section of the microjet device is 0.7~0.9; the •OH solution forms a large number of microbubbles in the diffusion section of the microjet device installed in the microjet injection tube, and •OH enters the breeding pond in a strong turbulent field and collapses internally to generate high temperature and high pressure, and quickly penetrates and diffuses into the surrounding liquid in the form of pressure microjets, 100% killing spores attached to the bottom of the pond, and the sprayed •OH solution is fully miscible and diffuses with the breeding water for 2~30 minutes, and 100% of parasites and microorganisms are killed when the concentration is greater than 0.5 mg / L, and organic matter and inorganic nitrogen in the breeding water are simultaneously mineralized, and 1~15 breeding ponds are treated simultaneously, achieving disinfection of the breeding pond while ensuring the health of the farmed fish.

7. The method for killing parasites in factory-farmed animals as claimed in claim 4, characterized in that: The microfilter / protein separator / biofilter device is composed of a microfilter, a protein separator and a biofilter device connected in series; a water distribution weir is installed inside the microfilter, and the aquaculture water enters the rotating drum through a guide pipe. A microporous filter with a mesh size of 60 to 100 per square inch is installed inside the drum to intercept the suspended matter in the aquaculture water into the drum, and the guide blades inside the filter cartridge guide the suspended particles to the mud discharge trough at the other end; the protein separator uses tiny bubbles released by a gas releaser to adsorb soluble macromolecular organic matter in the water and float it to the water surface, and separates it through a protein collector on the liquid surface; the bioreactor contains a three-dimensional hollow filler as a microbial carrier, the filler is a hollow structure, aerobic bacteria grow outside, and anaerobic bacteria grow inside the filler.

8. The method for killing parasites in factory-farmed animals as claimed in claim 4, characterized in that: When the treatment system is running, the intelligent control center uses the dynamic detection results of the water quality in the breeding pond and the total oxidant concentration in the pipeline as input information. Based on the established prediction model of the •OH solution in the breeding pond to kill parasites and the concentration diffusion model of •OH during the killing process, it dynamically controls the concentration and output of •OH solution produced by the hydroxyl radical generating equipment; dynamically detects and treats the •OH concentration in the breeding pond; and dynamically controls the opening and closing of the •OH micro-jet tubes in different breeding ponds.

Images