Anti-aging composition containing tricholoma matsutake and preparation method thereof

Abstract

The present application belongs to the technical field of skin care products, and discloses an anti-aging composition containing tricholoma matsutake and a preparation method thereof. The anti-aging composition comprises the following components: tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide, ginseng extract, chlorella extract, surfactant and water. The mass percentage of each component is as follows: 0.2% to 1% of tricholoma matsutake fermentation filtrate, 0.5% to 2% of retinol, 2% to 8% of collagen, 15% to 25% of ceramide, 1% to 5% of ginseng extract, 0.2% to 1% of chlorella extract, 0 to 4% of surfactant, and the balance of water. The anti-aging composition has the functions of fast absorption, obvious improvement of skin moisturizing, wrinkle resistance and skin tightening through the synergistic effect of the functional components of tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide and antioxidant plant extract.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to an anti-aging composition containing tricholoma matsutake and a preparation method thereof. BACKGROUND

[0002] Skin, as the largest human organ, is in contact with the external environment at all times and is constantly stimulated by the external environment. Under the action of external environmental pollutants, microorganisms and other stimulating factors such as sunlight, the skin will have different degrees of sensitization, such as dryness, itching, stinging, tightness and other symptoms, and problems such as aging, wrinkles, color spots, and darkening of skin color, which seriously affect skin health. With the improvement of people's living standards and the enhancement of maintenance awareness, skin health management has become increasingly important in people's daily life, and people will use various care and maintenance products to repair and maintain the skin, avoid more serious skin problems caused by external environmental stimulation, effectively protect skin health, and improve external appearance.

[0003] It is well known that free radicals and active oxygen produced by ultraviolet light not only induce acute inflammation such as sunburn, but also induce photoaging caused by long-term exposure to sunlight. It is believed that free radicals and active oxygen produced by ultraviolet light damage and change the cross-linking of elastic proteins such as collagen and elastin that make up the dermis, will inactivate antioxidant enzymes such as SOD, will cause peroxidation of cell membrane liposomes, and thus cause wrinkles.

[0004] In order to effectively delay skin aging, the countermeasures that can be taken in the field of cosmetics include: supplementing water and oil to maintain water and oil balance, repairing skin barrier function, inhibiting excessive free radical generation, promoting cell metabolism, supplementing skin collagen and elastin, preventing and repairing ultraviolet damage, inhibiting tyrosinase activity and melanin production, inhibiting non-enzymatic glycosylation, enhancing cell vitality, and promoting blood circulation. Patent CN115364038A discloses an anti-aging composition prepared by compounding yeast / bacillus licheniformis / fermented product of black tea, bacillus lacticum / fermented product filtrate of ginseng root, acetyl hexapeptide-8, dendrobium officinale extract, collagen, phloretin, retinoic acid ester, truffle extract, and other specific raw materials. After compounding the above-mentioned specific raw materials, the anti-aging effect is remarkable, and there is a synergistic effect. Patent CN116421482A discloses a composition containing tricholoma matsutake extract with anti-aging effect, which includes tricholoma matsutake extract, ergothioneine and bakuchiol. The composition has a good repair effect on UV-induced skin photoaging damage; when the components are compounded in different proportions, the efficacy is obviously better than that of each component used alone or two components used together, and there is a significant synergistic effect.

[0005] From the above prior art and the common knowledge in the art, it is known that the anti-aging functional components alone often cannot effectively exert their performance, and the effectiveness of their functions and good use experience often depend on reasonable component compounding. Therefore, it has great commercial value to develop an anti-aging composition containing Tricholoma matsutake with good formulation compatibility to meet different skin care product needs. SUMMARY

[0006] In view of the shortcomings and deficiencies of the above prior art, the primary object of the present application is to provide an anti-aging composition containing Tricholoma matsutake.

[0007] Another object of the present application is to provide a preparation method of the above anti-aging composition.

[0008] The object of the present application is achieved by the following technical solutions.

[0009] An anti-aging composition containing Tricholoma matsutake, comprising the following components: Tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide, Panax ginseng extract, Chlorella vulgaris extract, surfactant and water.

[0010] Preferably, the mass percentage content of each component in the anti-aging composition is as follows:

[0011] Tricholoma matsutake fermentation filtrate 0.2% to 1%;

[0012] Retinol 0.5% to 2%;

[0013] Collagen 2% to 8%;

[0014] Ceramide 15% to 25%;

[0015] Panax ginseng extract 1% to 5%;

[0016] Chlorella vulgaris extract 0.2% to 1%;

[0017] Surfactant 0 to 4%;

[0018] Water balance.

[0019] More preferably, the mass percentage content of each component in the anti-aging composition is as follows:

[0020] Tricholoma matsutake fermentation filtrate 0.5%;

[0021] Retinol 1%;

[0022] Collagen 5%;

[0023] Ceramide 20%;

[0024] Panax ginseng extract 3%;

[0025] Chlorella vulgaris extract 0.5%;

[0026] Surfactant 2%;

[0027] Water 68%.

[0028] Preferably, the Tricholoma matsutake fermentation filtrate is prepared by the following method:

[0029] After the Tricholoma matsutake is crushed, it is inoculated into a culture medium containing Brevibacterium casei for fermentation culture, and the obtained fermentation liquor is inactivated, filtered and purified to obtain the Tricholoma matsutake fermentation filtrate.

[0030] Further preferably, the components of the culture medium include: glucose 100-150 g / L, ammonium sulfate 0.3-0.8 g / L, NaCl 0.4-0.8 g / L, triammonium citrate 0.05-0.2 g / L, magnesium sulfate 0.1-0.4 g / L, and citric acid 1-3 g / L. The culture medium is natural pH and prepared with distilled water.

[0031] Further preferably, the fermentation culture temperature is 28-32°C, the time is 24-48 h, the aeration amount is 0.05-0.2 vvm, and the fermentation shaker speed is 150-200 r / min.

[0032] Preferably, the surfactant is a betaine type organosilicon zwitterionic surfactant, which is prepared by the following method:

[0033] The end-hydrogen-containing silicone oil and the allyl polyoxyethylene ether are added to an N-methylpyrrolidone solvent, a chloroplatinic acid catalyst is added, heated and stirred to react until the active hydrogen is completely reacted, then maleic anhydride is added for esterification, and then N,N-dimethyl-1,3-propanediamine and sodium ethoxide catalyst are added for amide reaction; the reaction product is vacuumed to remove unreacted raw materials and part of the solvent, then water and sodium chloroacetate are added for quaternary ammonium reaction, the reaction mixture is filtered, and the filtrate is vacuum dried to remove the solvent to obtain the betaine type organosilicon zwitterionic surfactant.

[0034] Further preferably, the molecular weight of the end-hydrogen-containing silicone oil is 500-5000; and the molecular weight of the allyl polyoxyethylene ether is 200-2000.

[0035] Further preferably, the reaction temperature of the reaction of adding the chloroplatinic acid catalyst to heat and stir is 75-90 DEG C, the reaction time is 1-4h; the reaction temperature of the esterification reaction is 70-80 DEG C, the reaction time is 1-2h; the reaction temperature of the amidation reaction is 120-160 DEG C, the reaction time is 2-6h; the reaction temperature of the quaternary ammonium reaction is 75-90 DEG C, the reaction time is 2-4h.

[0036] The preparation method of the anti-aging composition comprises the following preparation steps:

[0037] The surfactant is added to water and stirred to be dissolved uniformly, then the Tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide, Panax ginseng extract and Chlorella vulgaris extract are added and stirred to be mixed uniformly, to obtain the anti-aging composition.

[0038] Compared with the prior art, the anti-aging composition has the advantages that:

[0039] (1) The anti-aging composition has the functions of fast absorption, obvious moisturizing, anti-wrinkle and skin tightening, due to the synergistic effect of the Tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide and antioxidant plant extract functional components.

[0040] (2) The specific Tricholoma matsutake fermentation filtrate is used as the active component in the anti-aging composition, and has more obvious skin tightening effect than the conventional Tricholoma matsutake extract.

[0041] (3) The anti-aging composition further uses the specific betaine type organosilicon zwitterionic surfactant, which is a carboxylate betaine structure, is safe to the human body and mild to the skin, and has the zwitterionic structure which can be used with the anionic, cationic and nonionic surfactant systems, is stable to acid and alkali, and has good compatibility with various formulations. Meanwhile, the polysiloxane chain structure contained in the surfactant has low surface tension, good film forming performance, and can inhibit the generation of foam, and has better use skin feel than the conventional zwitterionic surfactant product when used as a skin care product. DETAILED DESCRIPTION

[0042] The application will be further described in detail below in combination with the examples, but the embodiments of the application are not limited thereto.

[0043] The Tricholoma matsutake fermentation filtrate used in the following examples is prepared by the following method:

[0044] Tricholoma matsutake was crushed and inoculated into a culture medium (the culture medium included: glucose 120 g / L, ammonium sulfate 0.5 g / L, NaCl 0.6 g / L, triammonium citrate 0.1 g / L, magnesium sulfate 0.2 g / L, citric acid 2 g / L, natural pH, prepared with distilled water; the strain was Brevibacterium casei 23944, at an inoculation amount of 2%; the fermentation was performed at a temperature of 30°C for 36 h; the ventilation amount of the fermentation was 0.1 vvm; the fermentation shaker speed was 180 r / min; the prepared fermentation filtrate was inactivated, filtered, and concentrated and purified by a nanofiltration membrane to obtain a Tricholoma matsutake fermentation filtrate.

[0045] Example 1

[0046] An anti-aging composition of the present example is composed of the following components in the following mass percentage:

[0047] Tricholoma matsutake fermentation filtrate 0.5%;

[0048] Retinol 1%;

[0049] Collagen 5%;

[0050] Ceramide 20%;

[0051] Panax ginseng extract 3%; (water-soluble ginsenoside CAS#: 90045-38-8; commercially purchased)

[0052] Chlorella vulgaris extract 0.5%; (Cas#: 223749-83-5; commercially purchased)

[0053] Water 70%.

[0054] The preparation method of the anti-aging composition is as follows: Tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide, Panax ginseng extract, and Chlorella vulgaris extract are sequentially added to hot water at 55-60°C, stirred and mixed uniformly, and cooled to obtain the anti-aging composition.

[0055] Example 2

[0056] An anti-aging composition of the present example is composed of the following components in the following mass percentage:

[0057] Tricholoma matsutake fermentation filtrate 0.2%;

[0058] Retinol 2%;

[0059] Collagen 8%;

[0060] Ceramide 15%;

[0061] Panax ginseng extract 5%;

[0062] Chlorella vulgaris extract 1%;

[0063] Water 68.8%.

[0064] The preparation method of the anti-aging composition is the same as that of Example 1.

[0065] Example 3

[0066] An anti-aging composition of this example is composed of the following components in mass percentage:

[0067] Tricholoma matsutake fermentation filtrate 1%;

[0068] Retinol 0.5%;

[0069] Collagen 2%;

[0070] Ceramide 25%;

[0071] Panax ginseng extract 1%;

[0072] Chlorella vulgaris extract 0.2%;

[0073] Water 70.3%.

[0074] The preparation method of the anti-aging composition is the same as that of Example 1.

[0075] Example 4

[0076] An anti-aging composition of this example is composed of the following components in mass percentage:

[0077] Tricholoma matsutake fermentation filtrate 0.5%;

[0078] Retinol 1%;

[0079] Collagen 5%;

[0080] Ceramide 20%;

[0081] Panax ginseng extract 3%;

[0082] Chlorella vulgaris extract 0.5%;

[0083] Betaine-type silicone zwitterionic surfactant 2%;

[0084] Water 68%.

[0085] The preparation method of the anti-aging composition is as follows: the betaine type organosilicon zwitterionic surfactant is added into water and stirred to be uniformly dissolved, then the tricholoma matsutake fermentation filtrate, retinol, collagen, ceramide, panax ginseng extract, and chlorella vulgaris extract are added and stirred to be uniformly mixed to obtain the anti-aging composition.

[0086] The betaine type organosilicon zwitterionic surfactant is prepared by the following method:

[0087] The end-hydrogen silicone oil with an average molecular weight of 2000 (the active hydrogen content is 0.1%) and the allyl polyoxyethylene ether with an average molecular weight of 800 are added into the N-methyl pyrrolidone solvent in a molar ratio of 1:2, a catalytic amount of chloroplatinic acid catalyst is added, and the temperature is increased to 80°C for stirring reaction for 3h until the active hydrogen is completely reacted, then the esterification reaction is performed by adding the allyl polyoxyethylene ether in an equal molar amount of maleic anhydride for 1h, and then the amidation reaction is performed by adding N,N-dimethyl-1,3-propanediamine (the molar ratio of maleic anhydride is 1.05:1) and sodium ethoxide catalyst and increasing the temperature to 125°C for 4h; the reaction product is subjected to vacuum removal of unreacted raw materials and part of the solvent, then water (the mass ratio of the remaining N-methyl pyrrolidone solvent is 1:1) and sodium chloroacetate (the molar ratio of N,N-dimethyl-1,3-propanediamine is 1:1) are added, and the quaternary ammonium reaction is performed at a temperature of 80°C for 2h, and then the reaction mixture is filtered, and the filtrate is dried by vacuum to obtain the betaine type organosilicon zwitterionic surfactant.

[0088] Comparative Example 1

[0089] Compared with Example 1, the same amount of tricholoma matsutake extract is used to replace the tricholoma matsutake fermentation filtrate.

[0090] The preparation method of the tricholoma matsutake extract is as follows: the dried tricholoma matsutake is added into 75% ethanol solution in a solid-liquid ratio of 1:10, and extracted at 80°C by water bath heating for 2h, and the extraction is performed twice, and then the tricholoma matsutake extract is obtained by filtration, concentration, and dilution with pure water.

[0091] Comparative Example 2

[0092] Compared with Example 1, the tricholoma matsutake fermentation filtrate is not added, the content of chlorella vulgaris extract is increased to 1%, and the rest is the same.

[0093] Comparative Example 3

[0094] The comparative example is compared with example 4, the betaine type organosilicon amphoteric ion surfactant is replaced with an equal amount of cocamide propyl betaine, and the rest is the same.

[0095] The compositions prepared in the above example 1, example 4 and comparative examples 1-3 were subjected to human anti-wrinkle, firming efficacy evaluation test, the test method is from: T / CAB0152-2022《Cosmetic Anti-wrinkle, Firming, Moisturizing, Oil Control, Repair, Nourishing, Seven Efficacy Test Methods》; Liu Weiwei, Zhou Lin, Zhao Hua. Cosmetic Efficacy Evaluation (XIII) - Subjective Use Test [J]. Daily Chemical Industry, 2021, 51(6): 6. Instrument testing (after using the sample for 28 days, testing the improvement rate of fish tail wrinkle area ratio (the measured value is significantly reduced, indicating that the fish tail wrinkle in unit area is significantly reduced, and it is considered that the sample has the anti-wrinkle effect of reducing fish tail wrinkle), skin elasticity R2 value improvement rate (the measured value is increased, indicating that the higher the skin elasticity, the sample has the effect of increasing skin elasticity) and skin firmness F4 value improvement rate (the measured value is reduced, indicating that the better the skin firmness, the sample has the effect of firming the skin)) and subjective evaluation by subjects (33 subjects, after using the sample for 28 days, the proportion of subjects with obvious corresponding improvement effect is calculated) were combined. The test results are shown in Tables 1 and 2, respectively.

[0096] Table 1. Instrument test conclusion

[0097]

[0098] From the comparison results of comparative examples 1-2 and example 1 in Table 1, compared with not adding the truffle fermentation filtrate and using truffle extract, the present application can significantly improve the firming effect of the composition by using the truffle fermentation filtrate in combination with retinol, collagen, ceramide, ginseng extract and chlorella extract. From the comparison results of example 4 and example 1, by further adding betaine type organosilicon amphoteric ion surfactant, it has certain enhancing effect on the anti-wrinkle effect of the composition in addition to enhancing the compatibility of the formula. From the results of comparative example 3, it can be seen that the conventional cocamide propyl betaine amphoteric surfactant has no significant effect on the anti-wrinkle effect and firming effect of the composition.

[0099] Table 2. Subjective evaluation conclusion

[0100]

[0101]

[0102] As can be seen from the results in Table 2, the subjective self-evaluation results of Comparative Examples 1-2 in wrinkle resistance and skin tightening are basically consistent with the instrument test results. Example 4 has obvious gains in improving skin dryness and dandruff and improving skin elasticity after adding the betaine-type silicone zwitterionic surfactant. The subjective self-evaluation results of Comparative Example 3 are significantly reduced after adding the conventional cocamidopropyl betaine zwitterionic surfactant, which is quite different from the instrument test results. The reason is that the conventional surfactant has certain foaming property and poor film-forming property, resulting in a significant reduction in actual use experience.

[0103] Comparative Example 4

[0104] This comparative example is compared with Example 4, the betaine-type silicone zwitterionic surfactant is replaced with an equal amount of a mixture of polydimethylsiloxane (average molecular weight 2000) and cocamidopropyl betaine (mass ratio of polydimethylsiloxane to cocamidopropyl betaine 1:1), and the rest is the same.

[0105] It is found during the preparation process that the anti-aging composition cannot be mixed into a uniform phase, there is obvious oil floating and stratification, and it cannot be further used normally. The reason is that the polydimethylsiloxane separates and precipitates in the composition.

[0106] The above examples are the preferred embodiments of the present application, but the embodiments of the present application are not limited by the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application should be equivalent replacement methods, and are all included in the protection scope of the present application.

Claims

1. An anti-aging composition containing matsutake mushroom, characterized in that, It includes the following components: matsutake fermentation filtrate, retinol, collagen, ceramide, ginseng extract, chlorella extract, surfactant, and water; The mass percentage composition of each component in the anti-aging composition is as follows: Matsutake fermentation filtrate: 0.2%~1%; Retinol 0.5%~2%; Collagen 2%~8%; Ceramide 15%~25%; Ginseng extract 1%~5%; Chlorella extract 0.2%~1%; Surfactant 2~4%; Water balance; The matsutake fermentation filtrate was prepared by the following method: After being crushed, matsutake mushrooms were inoculated into a culture medium containing Brevibacterium casei for fermentation. The resulting fermentation broth was then inactivated, filtered, and purified to obtain matsutake fermentation filtrate. The fermentation culture temperature was 28-32℃, the time was 24-48 h, the aeration rate was 0.05-0.2 vvm, and the fermentation shaking speed was 150-200 r / min. The ginseng extract refers to the ginseng extract with CAS number 90045-38-8; The Chlorella extract refers to the Chlorella extract with CAS number 223749-83-5; The surfactant is a betaine-type organosilicon zwitterionic surfactant, which is prepared by the following method: Hydrogen-containing silicone oil and allyl polyoxyethylene ether were added to N-methylpyrrolidone solvent. Chloroplatinic acid catalyst was added and the mixture was heated and stirred until the active hydrogen was completely reacted. Then, maleic anhydride was added for esterification. N,N-dimethyl-1,3-propanediamine and sodium ethoxide catalyst were added and the mixture was heated for amidation. The reaction product was subjected to vacuum to remove unreacted raw materials and some solvent. Then, water and sodium chloroacetate were added for quaternization. The reaction mixture was filtered, and the filtrate was dried under vacuum to remove the solvent, yielding a betaine-type organosilicon zwitterionic surfactant.

2. The anti-aging composition containing matsutake mushroom according to claim 1, characterized in that, The mass percentage composition of each component in the anti-aging composition is as follows: Matsutake fermentation filtrate: 0.5%; Retinol 1%; Collagen 5%; Ceramide 20%; 3% ginseng extract; Chlorella extract 0.5%; Surfactant 2%; Water 68%.

3. The anti-aging composition containing matsutake mushroom according to claim 1, characterized in that, The culture medium comprises: glucose 100-150 g / L, ammonium sulfate 0.3-0.8 g / L, NaCl 0.4-0.8 g / L, triammonium citrate 0.05-0.2 g / L, magnesium sulfate monohydrate 0.1-0.4 g / L, and citric acid 1-3 g / L.

4. The anti-aging composition containing matsutake mushroom according to claim 1, characterized in that, The molecular weight of the hydrogen-terminated silicone oil is 500-5000; the molecular weight of the allyl polyoxyethylene ether is 200-2000.

5. The anti-aging composition containing matsutake mushroom according to claim 4, characterized in that, The reaction temperature for the addition of chloroplatinic acid catalyst and heating and stirring is 75~90℃, and the reaction time is 1~4h; the reaction temperature for the esterification reaction is 70~80℃, and the reaction time is 1~2h; the reaction temperature for the amidation reaction is 120~160℃, and the reaction time is 2~6h; the reaction temperature for the quaternization reaction is 75~90℃, and the reaction time is 2~4h.

6. A method for preparing an anti-aging composition according to any one of claims 1 to 5, characterized in that, The preparation steps include the following: The surfactant was added to water and stirred until dissolved. Then, matsutake fermentation filtrate, retinol, collagen, ceramide, ginseng extract, and chlorella extract were added and stirred until well mixed to obtain an anti-aging composition.

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