A method for releasing contents by targeting / destroying dead bacteria using magnetic nanoparticles

By using a combination of functionalized magnetic nanoparticles and low-frequency magnetic fields in bioreactors, the structure of dead bacteria is destroyed and the contents are released, which solves the problem of low utilization of dead bacteria in traditional bioreactors and improves reactor efficiency and products. Yield.

CN117384761BActive Publication Date: 2025-01-24BEIJING FORESTRY UNIVERSITY +1
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Patent Information

Application Number
CN202311218798.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-21
Publication Date
2025-01-24
Estimated Expiration
2043-09-21

AI Technical Summary

Technical Problem

The low utilization rate of dead bacteria in traditional bioreactors leads to high economic and environmental costs, and it is difficult to effectively release the contents of dead bacteria.

Method used

Using functional magnetic nanoparticles, through the combination of high -friendly nuclear force in the nuclear kernel area, under the inducement of low -frequency magnetic fields, the structure of the damage to the deadly bacteria was used to release the content of the content.

Benefits of technology

The concentration of the substrate of the biological reactor is increased, the amount of residue is generated, the product production rate is increased, and the high recovery rate has been achieved, and it has environmental-economic benefits.

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Abstract

The present invention relates to the technical field of microbial reactors, and in particular to a method for using magnetic nanoparticles to target / destroy dead bacteria and release contents. The method of the present invention comprises the following steps: adding functionalized magnetic nanoparticles to a bioreactor containing dead bacteria and mixing them to obtain a mixed system; applying a magnetic field to the mixed system for treatment. The present invention places functionalized magnetic nanoparticles in a bioreactor containing dead bacteria and fully mixes them, and the functionalized magnetic nanoparticles have a greater probability of entering the dead cells through the pores in the dead bacterial cell walls; then a low-frequency magnetic field with a certain magnetic field strength and frequency is used to remotely control the nanoparticles entering the dead bacteria to move according to a specific trajectory and amplitude, and the functionalized nanoparticles entering the dead bacteria can use mechanical force from the inside to break the dead bacterial cell walls and release the contents, thereby increasing the substrate concentration of the bioreactor, reducing the amount of residue generated, and increasing the product yield.
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Citation Information

Patent Citations

  • Nucleolus target fluorescence carbon point as well as preparation method and application thereof

    CN107118765A

  • Method for specifically targeting / destroying infectious microbes by using surface-functionalized magnetic nanoparticles

    CN116555038A