A process for the preparation of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid

Abstract

The application relates to the technical field of biological buffer reagent synthesis, and discloses a preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, which comprises the following preparation steps: compound 1 is reacted with reagent A to obtain compound 2; the compound 2 is reacted with sulfonating reagent B to obtain compound 3; and the compound 3 is subjected to at least one of reaction with an acid or an alkali and hydrolysis reaction to prepare N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid; the raw material used in the preparation method is low in cost, simple in operation, and high in product purity; the product yield is improved, the product application cost is reduced, the product is suitable for industrial production, and the obtained product can meet the requirements of the biological buffer field on purity, impurity content and cost.

Description

Technical Field

[0001] This invention belongs to the field of biological buffer technology and relates to a preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid. Background Technology

[0002] In biological research, research subjects are typically cultured in a culture medium. During chemical reactions, the cellular media, interstitial spaces, and biomolecules in the culture medium react with [H+]. + The concentration has certain requirements, therefore a suitable biological buffer with a relatively stable pH value is needed. In addition to a suitable pKa value, biological buffers are usually required to be chemically inert, readily soluble in water but sparingly soluble in organic solvents, minimally affected by temperature and with a small salt effect, and also easy to synthesize.

[0003] Hydroxyethylaminosulfonate (BES) is a promising biobuffer. Traditional synthetic routes use diethanolamine and sodium butene sulfonate or sodium chloroethylsulfonate as reactants, resulting in a low yield of only about 60%, leading to low production efficiency, expensive raw materials, high energy consumption, and significant wastewater discharge. Chinese patent CN201510338904.X discloses a method for preparing a biobuffer, breaking away from the traditional method using diethanolamine and sodium butene sulfonate or sodium chloroethylsulfonate as raw materials. This method involves the reaction of ethylene oxide and aminosulfonate to obtain BES, significantly improving the reaction yield and efficiency and overcoming the problems of low yield and efficiency in existing methods. Furthermore, the synthesis process uses less solvent, generates less wastewater, has high reaction efficiency, and uses readily available and simple raw materials, making it easy to industrialize. However, this patent involves the removal of sodium ions using a strongly acidic ion exchange resin to obtain BES. The activation process of the strongly acidic ion exchange resin is complex, generating a large amount of waste, resulting in low efficiency, a long process, high commercialization costs, and low operability.

[0004] In general, there are relatively few patents for N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid and its sodium salt. Among the existing patents, the preparation processes still have several problems, such as: difficulty in obtaining raw materials, high manufacturing costs, low yields, excessive waste, and complex preparation methods. If these problems are not resolved, their large-scale application will be hindered, especially their development as materials in the field of biological buffers. Summary of the Invention

[0005] To overcome the shortcomings of the prior art, the present invention aims to provide a preparation process for N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid. By optimizing the starting materials, preparation method, and purification process, a high-purity product is obtained and the yield is improved. The preparation process of the present invention is simple, low-cost, and suitable for industrial production, and is suitable for application in the field of biological buffers.

[0006] The objective of this invention can be achieved by adopting the following technical solutions:

[0007] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following steps:

[0008] Preparation step S1: Compound 1 reacts with reagent A to prepare compound 2;

[0009] Preparation step S2: Compound 2 reacts with sulfonating agent B to prepare compound 3;

[0010] Preparation step S3: Compound 3 is reacted with an acid or base or hydrolyzed to prepare N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid;

[0011] The structural formula of compound 1 is shown in Formula I:

[0012]

[0013]

[0014] The structural formula of compound 2 is shown in Formula II:

[0015]

[0016] The reaction equation for preparation step S1 is shown in equation (1) below:

[0017]

[0018] The structural formula of compound 3 is shown in Formula III:

[0019]

[0020] The reaction equation for preparation step S2 is shown in equation (2) below:

[0021]

[0022] The reaction equation for preparation step S3 is shown in equation (3) below:

[0023]

[0024] Wherein, reagent A is at least one of halogenating reagent, RCOOH, RCOX, RSO3H, and RSO2X; X is a halogen;

[0025] X1, X2, and X3 are at least one of halogen, OH, OSO2R, and OCOR; and not all of X1, X2, and X3 are OH.

[0026] M is a Group IA or Group IIA metal, NH4 + At least one of them;

[0027] R is a C1-C bond that is saturated or unsaturated, contains straight or branched chains, and contains or does not contain heteroatoms. 30 Hydrocarbon group, C1-C 30 One of the aromatic ring groups.

[0028] Furthermore, in preparation step S1, after compound 1 reacts with reagent A, it undergoes at least one hydrolysis reaction or esterification reaction to prepare compound 2.

[0029] Furthermore, the sulfonating agent B is at least one of the following: sulfite, bisulfite, concentrated sulfuric acid, fuming sulfuric acid, chlorosulfonic acid, sulfur trioxide, SO2, and a reaction mixture of an oxide or hydroxide of an alkali metal group IA or a group IIA alkaline earth metal.

[0030] Furthermore, the halogenating agent is at least one of halogen, hydrogen halide, hydrohalic acid, sulfur-containing halide, and phosphorus-containing halide.

[0031] Furthermore, the reaction temperature in preparation step S1 is -50 to 200°C, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours.

[0032] The reaction temperature in preparation step S2 is -50 to 200°C, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours.

[0033] The reaction temperature in preparation step S3 is -50 to 200℃, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours.

[0034] Furthermore, in preparation step S1, the molar ratio of compound 1 to reagent A is 1:(0.1-10);

[0035] In preparation step S2, the molar ratio of compound 2 and sulfonating agent B is 1:(0.1-10).

[0036] Furthermore, the preparation step S1 is carried out in reaction solvent A, wherein reaction solvent A is one or a combination of two or more of the following: acetone, dichloromethane, trichloromethane, dichloroethane, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

[0037] Preparation step S2 is carried out in reaction solvent B, wherein reaction solvent B is one or a combination of two or more of methanol, ethanol, acetone, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

[0038] Preparation step S3 involves reacting in reaction solvent C, wherein reaction solvent C is one or a combination of two or more of methanol, ethanol, acetone, dichloromethane, trichloromethane, dichloroethane, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

[0039] Furthermore, a preparation process for N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid also includes the following preparation steps: recrystallizing the obtained N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purified solvent to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid.

[0040] Furthermore, the purification solvent is one or a combination of two or more of methanol, ethanol, acetone, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

[0041] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0042] 1. This application provides a method for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid. By optimizing the starting materials and the preparation and purification steps, the yield of the product is improved. The method is simple, reduces the cost of the product, and is suitable for industrial production. The N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid obtained has high purity, which meets the requirements of the field of biological buffers for purity, impurity content, and cost, and is suitable for application in the field of biological buffers.

[0043] 2. The byproducts and impurities generated by the preparation method of this application are easy to purify and separate. The impurities in the product do not need to go through a complicated purification process to achieve the high purity standard for practical applications. This simplifies the product preparation process, makes the product highly pure, and can meet the production and quality requirements of large-scale applications. Detailed Implementation

[0044] The present invention will now be further described in conjunction with specific embodiments. It should be noted that these specific embodiments are provided to illustrate the invention in more detail through some non-limiting specific implementations. However, the present invention is not limited to the following embodiments; the embodiments and descriptions in the specification are merely illustrative of the principles of the invention. That is, the following descriptions are only some preferred embodiments of the present invention and should not be construed as limiting the scope of the invention. For those skilled in the art, various changes, improvements, and modifications can be made to the present invention without departing from its spirit, principles, and scope. These additional features of the improvements can exist individually or in any combination, and these changes, improvements, and modifications should also be considered within the scope of the invention as claimed. Furthermore, the raw materials used in the present invention are generally commercially available products, therefore, there is no need to specifically limit their source.

[0045] Nuclear magnetic resonance (NMR) analysis was performed using a Bruker AVANCE 400 MHz NMR spectrometer.

[0046] Purity was analyzed and tested by high performance liquid chromatography (HPLC).

[0047] Unless otherwise specified, the pressure values ​​mentioned in this patent application refer to gauge pressure, which is the total absolute pressure exceeding the surrounding atmospheric pressure or the pressure at a certain point in a liquid that exceeds atmospheric pressure.

[0048] Unless otherwise specified, the reaction temperature generally refers to the oil bath temperature of the reaction.

[0049] Yield is the percentage ratio of actual product quality to theoretical product quality. Theoretical product quality is calculated using raw materials that are not excessive in the reaction equation.

[0050] Example 1

[0051] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following preparation steps:

[0052] (1) Preparation step 1:

[0053]

[0054] In a reactor, 1 mole of compound 1 was reacted with thionyl chloride; the molar ratio of compound 1 to thionyl chloride was 1:1.1, the solvent was dichloromethane, the reaction temperature was 40°C, the reaction pressure was atmospheric pressure, and the reaction time was 4 hours. After the reaction was completed, the mixture was evaporated to dryness to obtain compound 2.

[0055] (2) Preparation step 2:

[0056]

[0057] In a reactor, 1 mole of compound 2 from the above formula was reacted with sodium sulfite; the molar ratio of compound 2 to sodium sulfite was 1:1.1; the solvent was DMF; the reaction temperature was 110℃; the reaction pressure was atmospheric pressure; the reaction time was 1 hour; after the reaction was completed, the solvent was evaporated to obtain compound 3.

[0058] (3) Preparation step 3:

[0059]

[0060] In a reactor, 1 mole of compound 3 from the above formula was dissolved in methanol, and excess hydrochloric acid solution was added. The acidification reaction was carried out under normal pressure for 1 hour. After the reaction was completed, the solvent was evaporated to obtain N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a crude product yield of 92%.

[0061] (4) Purification steps:

[0062] In a reactor, 1 mole of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was dissolved in methanol, and ethyl acetate was added as a purification solvent for recrystallization. The insoluble matter was obtained by filtration and drying to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purification yield of 90% and a product purity of 99.2%.

[0063] The nuclear magnetic resonance characterization data are as follows:

[0064] 1 HNMR(400MHz,D2O):3.883(4H),3.664(2H),3.402(4H),3.317(2H).

[0065] Example 2

[0066] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following preparation steps:

[0067] (1) Preparation step 1:

[0068]

[0069] In a reactor, 1 mole of compound 1 was reacted with propionic acid; a small amount of sulfuric acid was added to remove the water produced in the reaction. The molar ratio of compound 1 to propionic acid was 1:1.2. The solvent was tetrahydrofuran. The reaction temperature was 50°C, the reaction pressure was atmospheric pressure, and the reaction time was 3 hours. After the reaction was completed, the mixture was evaporated to dryness to obtain compound 2.

[0070] (2) Preparation step 2:

[0071]

[0072] In a reactor, 1 mole of compound 2 from the above formula was reacted with sulfonating agent B, which was a reaction mixture of SO2 and sodium hydroxide; the molar ratio of compound 2 to sulfonating agent B was 1:1.05; the solvent was tetrahydrofuran; the reaction temperature was 65°C; the reaction pressure was atmospheric pressure; and the reaction time was 2 hours. After the reaction was completed, the solvent was evaporated to obtain compound 3.

[0073] (3) Preparation step 3:

[0074]

[0075] In a reactor, 1 mole of compound 3 from the above formula was dissolved in ethanol, and then excess hydrochloric acid solution was added. The acidification reaction was carried out under normal pressure for 1 hour. After the reaction was completed, the solvent was evaporated to obtain crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a crude product yield of 93%.

[0076] (4) Purification steps:

[0077] In a reactor, 1 mole of crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was dissolved in ethanol, and dimethyl carbonate was added as a purification solvent for recrystallization. The insoluble matter was obtained by filtration and dried to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purification yield of 92% and a product purity of 99.1%.

[0078] The nuclear magnetic resonance characterization data are as follows:

[0079] 1 H NMR (400MHz, D2O): 3.883(4H), 3.664(2H), 3.402(4H), 3.317(2H).

[0080] Example 3

[0081] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following preparation steps:

[0082] (1) Preparation step 1:

[0083]

[0084] In a reactor, 1 mole of compound 1 was reacted with acetyl chloride; the molar ratio of compound 1 to acetyl chloride was 1:1.2, the solvent was acetonitrile, the reaction temperature was 40°C, the reaction pressure was atmospheric pressure, and the reaction time was 4 hours. After the reaction was completed, the mixture was evaporated to dryness to obtain compound 2.

[0085] (2) Preparation step 2:

[0086]

[0087] In a reactor, 1 mole of compound 2 from the above formula was reacted with sodium bisulfite; the molar ratio of compound 2 to sodium bisulfite was 1:1.05; the solvent was tetrahydrofuran; the reaction temperature was 45°C; the reaction pressure was atmospheric pressure; the reaction time was 3 hours; after the reaction was completed, the solvent was evaporated to obtain compound 3.

[0088] (3) Preparation step 3:

[0089]

[0090] In a reactor, 1 mole of compound 3 from the above formula was dissolved in DMF, and then excess hydrochloric acid solution was added. The acidification reaction was carried out under normal pressure for 2 hours. After the reaction was completed, the solvent was evaporated to obtain crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a crude product yield of 90%.

[0091] (4) Purification steps:

[0092] In a reactor, 1 mole of crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was dissolved in methanol, and the solution was recrystallized by adding diethyl ether as a purification solvent. The insoluble matter was filtered and dried to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purification yield of 91% and a product purity of 99.3%.

[0093] The nuclear magnetic resonance characterization data are as follows:

[0094] 1 H NMR (400MHz, D2O): 3.883(4H), 3.664(2H), 3.402(4H), 3.317(2H).

[0095] Example 4

[0096] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following preparation steps:

[0097] (1) Preparation step 1:

[0098]

[0099] In a reactor, 1 mole of compound 1 was reacted with ethylsulfonyl chloride; the molar ratio of compound 1 to ethylsulfonyl chloride was 1:1.2, the solvent was DMF, the reaction temperature was 80°C, the reaction pressure was atmospheric pressure, and the reaction time was 1 hour. After the reaction was completed, the mixture was evaporated to dryness to obtain compound 2.

[0100] (2) Preparation step 2:

[0101]

[0102] In a reactor, 1 mole of compound 2 from the above formula was reacted with sodium sulfite; the molar ratio of compound 2 to sodium sulfite was 1:1.1; the solvent was tetrahydrofuran; the reaction temperature was 60°C; the reaction pressure was atmospheric pressure; the reaction time was 1 hour; after the reaction was completed, the solvent was evaporated to obtain compound 3.

[0103] (3) Preparation step 3:

[0104]

[0105] In a reactor, 1 mole of compound 3 from the above formula was dissolved in methanol, and then excess hydrochloric acid solution was added. The acidification reaction was carried out under normal pressure for 1.5 hours. After the reaction was completed, the solvent was evaporated to obtain crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a crude product yield of 87%.

[0106] (4) Purification steps:

[0107] In a reactor, 1 mole of crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was dissolved in methanol, and acetone was added as a purification solvent for recrystallization. The insoluble matter was obtained by filtration and drying to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purification yield of 93% and a product purity of 99.0%.

[0108] The nuclear magnetic resonance characterization data are as follows:

[0109] 1 H NMR (400MHz, D2O): 3.883(4H), 3.664(2H), 3.402(4H), 3.317(2H).

[0110] Example 5

[0111] A process for preparing N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid includes the following preparation steps:

[0112] (1) Preparation step 1:

[0113]

[0114] In a reactor, 1 mole of compound 1 was reacted with phosphorus tribromide; the molar ratio of compound 1 to phosphorus tribromide was 1:3.15, the solvent was dioxane, the reaction temperature was 70°C, the reaction pressure was atmospheric pressure, and the reaction time was 1.5 hours. After the reaction was completed, the mixture was evaporated to dryness to obtain compound 2.

[0115] (2) Preparation step 2:

[0116]

[0117] In a reactor, 1 mole of compound 2 from the above formula was reacted with sodium bisulfite; the molar ratio of compound 2 to sodium bisulfite was 1:1.25; the solvent was DMF; the reaction temperature was 80°C; the reaction pressure was atmospheric pressure; and the reaction time was 0.5 hours. After the reaction was completed, the solvent was evaporated to obtain compound 3.

[0118] (3) Preparation step 3:

[0119]

[0120] In a reactor, 1 mole of compound 3 from the above formula was dissolved in methanol, and 2.1 moles of aqueous sodium hydroxide solution were added. The mixture was stirred until the reaction was complete. Then, excess hydrochloric acid was added to carry out an acidification reaction under normal pressure for 2 hours. After the reaction was completed, the solvent was evaporated to obtain crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a crude product yield of 90%.

[0121] (4) Purification steps:

[0122] In a reactor, 1 mole of crude N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was dissolved in methanol, and tetrahydrofuran was added as a purification solvent for recrystallization. The insoluble matter was obtained by filtration and drying to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid with a purification yield of 92% and a product purity of 99.5%.

[0123] The nuclear magnetic resonance characterization data are as follows:

[0124] 1 H NMR (400MHz, D2O): 3.883(4H), 3.664(2H), 3.402(4H), 3.317(2H).

[0125] The above experiments show that the N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid prepared in the examples has high purity and low impurity content, which can meet the requirements of the application field. Moreover, the product yield of the preparation method described in this invention can reach up to 93%, and the product yield has also been improved, with the product purity reaching up to 99.5%.

[0126] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A preparation process for N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, characterized in that, Includes the following steps: Preparation step S1: Compound 1 reacts with reagent A to prepare compound 2; Preparation step S2: Compound 2 reacts with sulfonating agent B to prepare compound 3; Preparation step S3: Compound 3 is reacted with an acid or base or hydrolyzed to prepare N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid; The structural formula of compound 1 is shown in Formula I: The structural formula of compound 2 is shown in Formula II: The structural formula of compound 3 is shown in Formula III: Wherein, reagent A is at least one of halogenating reagent, RCOOH, RCOX, and RSO2X; X is a halogen; X1 and X2 are at least one of halogen and OH; X3 is at least one of halogen, OSO2R, and OCOR; M is at least one group IA metal; R is one of the saturated C1-C2 hydrocarbon groups containing a straight chain; The halogenating agent is at least one of halogen or phosphorus-containing halide; The sulfonating agent B is at least one of a sulfite or a bisulfite.

2. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 1, characterized in that, In preparation step S1, after compound 1 reacts with reagent A, it undergoes at least one hydrolysis reaction or esterification reaction to prepare compound 2.

3. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 1, characterized in that, The reaction temperature in preparation step S1 is -50 to 200°C, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours. The reaction temperature in preparation step S2 is -50 to 200°C, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours. The reaction temperature in preparation step S3 is -50 to 200℃, the reaction pressure is -0.05 to 1 MPa, and the reaction time is 0.1 to 72 hours.

4. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 1, characterized in that, In preparation step S1, the molar ratio of compound 1 to reagent A is 1:(0.1-10); In preparation step S2, the molar ratio of compound 2 and sulfonating agent B is 1:(0.1-10).

5. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 1, characterized in that, Preparation step S1 involves reacting in reaction solvent A, wherein reaction solvent A is one or a mixture of two or more of the following: acetone, dichloromethane, trichloromethane, dichloroethane, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide. Preparation step S2 is carried out in reaction solvent B, wherein reaction solvent B is one or a mixture of two or more of methanol, ethanol, acetone, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide. Preparation step S3 involves reacting in reaction solvent C, wherein reaction solvent C is one or a mixture of two or more of methanol, ethanol, acetone, dichloromethane, trichloromethane, dichloroethane, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

6. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 1, characterized in that, It also includes the following preparation steps: The obtained N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid was recrystallized with a purification solvent to obtain purified N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid.

7. The preparation process of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid according to claim 6, characterized in that, The purification solvent is one or a mixture of two or more of methanol, ethanol, acetone, tetrahydrofuran, ethyl acetate, dimethyl carbonate, diethyl carbonate, diethyl ether, acetonitrile, dioxane, N,N-dimethylformamide, and dimethyl sulfoxide.

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