Bee artificial insemination teaching system

Abstract

The application discloses a bee artificial insemination teaching system, which comprises a carbon dioxide unified gas supply system, a precise cold light illumination system, an equal proportion silica gel model, a color marking adjusting knob, a multi-channel synchronous display system and an insemination step precision unit. The carbon dioxide unified gas supply system comprises a carbon dioxide tank, a pipeline network, a system total switch, independent switches, a carbon dioxide bubble counter and independent flow rate adjusting knobs. The multi-channel synchronous display system comprises an operator display, an integrator, a converter, a large display and a computer remote online conference. The insemination step precision unit comprises a semen collection module, a queen bee processing module and an artificial insemination module. The application constructs a teaching system based on popularization of bee artificial insemination technology, perfects the content of bee artificial insemination teaching, improves operation efficiency and convenience through unified gas supply, significantly reduces storage demand and safety hazards, and can meet the demand of various different scenes through a multi-channel display mode and can accurately process student problems.

Description

Technical Field

[0001] This invention relates to the field of beekeeping teaching technology, and in particular to a bee artificial insemination teaching system. Background Technology

[0002] Honeybees are among the most important pollinating insects, possessing immense economic and ecological value. As one of the very few special economic resource insects that can be artificially domesticated, approximately 85% of flowering plants rely on honeybees for pollination, making their pollination function irreplaceable. The ecological and commercial value brought about by pollination is immeasurable.

[0003] Artificial insemination of bees is the process of obtaining drone semen with the aid of instruments and injecting it into the queen bee. It mainly includes semen collection, queen bee treatment, and artificial insemination. Artificial insemination is currently an effective method for bee breeding and preservation. It allows for precise control over the breed and physiological characteristics of the desired drones. By accurately selecting the paternal and maternal parents, individuals with clear genetic backgrounds, good health, and superior traits can be identified for breeding, avoiding hybridization and ensuring the reproduction of purebred bees, thus maintaining the genetic purity of the population. It can also maintain or improve the genetic characteristics of specific breeds, such as disease resistance, bee behavior, and honey production capacity. Experiments have shown that bee colonies bred by artificially inseminated queens do not differ significantly in performance from those bred by naturally inseminated queens and can be used to produce colonies with the same phenotype. Even, under proper conditions, artificially inseminated colonies can exhibit superior vitality and productivity compared to naturally inseminated colonies. Furthermore, natural bee reproduction is severely constrained by many factors such as season, geographical environment, and climate, easily missing flowering periods and requiring a long breeding time. Artificial insemination allows for flexible timing of breeding, enabling earlier queen rearing and swarming, extending the bee breeding season, and improving breeding efficiency. Furthermore, through the selection of sires, artificial insemination accelerates genetic selection in bees, significantly promoting population genetic improvement and speeding up the breeding process. It is an indispensable technique in modern bee management and breeding.

[0004] A common basic model of modern artificial insemination equipment for bees includes a queen confinement chamber with carbon dioxide anesthesia, an abdominal hook for pulling the abdominal plates, a dorsal hook for pulling the dorsal plates, and a syringe for injecting drone semen. The syringe consists of a screw, a conical needle, and a plastic tube that continuously collects and injects semen by pushing the screw. Because artificial insemination of bees is a complex technique requiring specialized knowledge and skills, its widespread application is limited. The number of people proficient in this technique is limited, and teaching and explaining it is also challenging. Since actual demonstrations and operations require microscope assistance, conveying key information through text or video is not ideal. Furthermore, the information seen under the microscope may not be synchronized with the actual operation, leading to difficulties in conveying crucial information. For example, the semen collection location, volume, and insemination location cannot be accurately communicated to students, resulting in low teaching efficiency for artificial insemination. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a teaching system for artificial insemination of bees.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] The artificial insemination teaching system for bees includes a unified carbon dioxide supply system, a precise cold light illumination system, a proportional silicone model, a color-marking adjustment knob, a multi-channel synchronous display system, and a precise insemination step unit. The unified carbon dioxide supply system includes a carbon dioxide tank, a pipeline network, a system master switch, independent switches, a carbon dioxide bubble counter, and an independent flow rate adjustment knob. The precise cold light illumination system includes a global illumination module and a local illumination module. The proportional silicone model includes proportional silicone models of drones and queen bees. The multi-channel synchronous display system includes an operator display, an integrator, a converter, a large display, and a computer-based remote online conferencing system. The precise insemination step unit includes a semen collection module, a queen bee processing module, and an artificial insemination module.

[0008] The semen collection module includes three steps: drone breeding, forced ejaculation, and semen collection. The queen bee treatment module includes two steps: selection of virgin queen bees and queen bee anesthesia. The artificial insemination module includes two steps: opening the stinger cavity and inserting the needle for injection.

[0009] As a further technical solution of the present invention, the unified carbon dioxide supply system connects carbon dioxide tanks to twenty or more seats through a pipeline network. The main switch of the system is controlled before and after the start of teaching. Each student's desk is equipped with an independently designed switch, a carbon dioxide bubble meter for controlling the flow rate, and a carbon dioxide flow rate adjustment knob.

[0010] As a further technical solution of the present invention, the global illumination module consists of a global illumination lamp located inside the microscope, and the local illumination module consists of a magnetic LED spotlight fixed to a specific part of the microscope and connected externally. Both the global illumination lamp and the magnetic LED spotlight are low-energy cold light lamps that do not affect the activity of bee sperm.

[0011] As a further technical solution of the present invention, the proportional silicone model is made by resin casting and 3D printing, wherein the male bee's reproductive organs and the queen bee's sting chamber are independently movable.

[0012] As a further technical solution of the present invention, the color-marked adjustment knob uses different colors to independently mark commonly used adjustment knobs, which facilitates the issuance of accurate instructions by color during teaching, distinguishes different operations, and makes it easier for students to remember.

[0013] As a further technical solution of the present invention, the operator display adopts a 10-inch small high-definition display, and the large display adopts a 120-inch high-resolution large screen display.

[0014] As a further technical solution of the present invention, the multi-channel synchronous display system is as follows: each microscope is connected to an independent 10-inch small high-definition display, the display content of each microscope is integrated into a converter through an integrator, and transmitted to a 120-inch high-resolution large screen display through multi-screen projection. At the same time, remote synchronous observation and learning can be realized with the help of computer remote online conferencing technology.

[0015] As a further technical solution of the present invention, the semen collection module includes the following specific steps:

[0016] a) Drone rearing: Through proper organization of the bee colony, the queen bee of the paternal colony lays drone eggs on the drone comb. After 24 days, the drones emerge from their cells and are confined in drone cages and raised in the hive. The drones reach sexual maturity about 15 days after emergence. Ensure that there are enough nurse bees in the colony to raise healthy, sexually mature drones.

[0017] b) Forced ejaculation: Hold the male bee with your left hand and gently press its thorax. A sexually mature male bee will have a hardened and contracted abdomen. Apply pressure to the abdomen with your thumb or forefinger to force the inner penis to turn outward. Stop squeezing when a pale yellow spherical shape emerges from the ejaculation tube. The pale yellow viscous liquid outside the penis ball is the semen. Note that the pressure should be stable, and squeeze in small amounts multiple times to avoid the penis ball from rupturing or the semen from splashing out. During the operation, avoid contact between the penis ball and the semen on the surface of the male bee or your fingers to prevent semen contamination.

[0018] c) Semen collection: Draw a section of saline solution into the needle to keep the needle tip moist. Fix the hook and syringe to the insemination device. When collecting semen, first draw in about 1 cm of air to separate the saline solution from the semen. Keep the drone semen close to the needle tip to avoid drawing in air. Rotate the drone with your left hand and operate the syringe knob with your right hand to draw in the semen. Collect about 1 μL of semen from each drone. After collecting from a single drone, seal the semen in the order of "semen-air column-saline column". When collecting from the next drone, push out the air column and saline column to continue collecting. Push out a portion of the previous drone's semen outside the needle tip, let it come into contact with the semen surface, and then draw it in. After collecting the semen from 8-10 drones to form a semen column, collect the next semen column by separating it into an air column-saline column-air column.

[0019] As a further technical solution of the present invention, the queen bee processing module includes the following specific steps:

[0020] a) Selection of virgin queen bees: Virgin queen bees should be cultivated and selected in advance for artificial insemination. Generally, virgin queen bees with long abdomens, steady movements, and larger size are selected. The best time for insemination is 7-10 days old.

[0021] b) Queen anesthesia: Hold the queen bee's thorax with your right hand, with the induction tube opening downwards, and insert the queen bee's head into the induction tube. After the queen bee instinctively crawls upwards into the tube, connect it to the anesthesia tube. Connect the anesthesia tube to the holder on the base of the artificial insemination device, so that 3-4 segments of the queen bee's abdomen are exposed. If the queen bee does not retreat on her own, gently tap the anesthesia tube to make it fall in, avoiding violent disturbance. Adjust the rate by adjusting the carbon dioxide flow rate knob and observe the bubble flow in the carbon dioxide bubble counter to form a stable bubble flow, preferably five bubbles per second. Anesthetize until the queen bee no longer struggles violently and begins to breathe rhythmically and tremble. The virgin queen enters a coma, and insemination can begin. The anesthesia is lifted after insemination.

[0022] As a further technical solution of the present invention, the artificial insemination module includes the following specific steps:

[0023] a) Opening the sting chamber: After the queen bee enters a comatose state, use a pry bar to open the abdominal plate, move the operating rod to hook the dorsal hook onto the abdominal plate, use the pry bar to pick out the stinger, clamp the stinger with the dorsal plate clamp, pull it open in both directions in parallel, pull the stinger towards the back, so that the distance between the abdominal plate and the dorsal plate is 3-4mm, adjust the dorsal hook to pull the stinger out of the abdominal cavity, use the double rod to pull the sting chamber open in parallel to 5-6mm, exposing the vaginal opening on the side of the stinger base near the abdomen;

[0024] b) Injection: After opening the sting chamber, fine-tune the microscope focus to clearly display the inside of the sting chamber in the center of the field of view. Observe the folded depression about 1.5mm from the sting needle in the center of the sting chamber, which is the vaginal opening. Adjust the syringe angle so that it is parallel to the queen bee's longitudinal direction and makes a 30° angle with the vertical line to contact the queen bee. After finding the vaginal opening, expel the air and saline from the tip of the insemination needle and insert the needle at a 30° angle. When resistance is felt, it indicates that the needle tip has touched the vaginal valve. Turn the control syringe knob counterclockwise until the insemination needle naturally penetrates 0.5-0.8mm, indicating successful insemination. After successfully penetrating the queen bee's pelvis, insert the needle 1mm further to ensure successful insertion. Once the insertion is confirmed, rotate the syringe control knob to inject semen. Typically, 8-10μL of semen is injected at once. If necessary, a second insemination can be performed 24-48 hours later, injecting 4-5μL of semen. After injection, withdraw the needle to release the queen bee's restriction. Once the queen bee has recovered, return her to the mating box and observe whether fertilization was successful.

[0025] The beneficial effects of this invention are: it constructs a teaching system based on promoting artificial insemination technology for bees, improves the content of artificial insemination teaching for bees, and covers all aspects such as breeding bee colony organization and management, key points of insemination technology, and troubleshooting;

[0026] The innovative unified carbon dioxide gas supply system not only improves operational efficiency and convenience, but also significantly reduces storage requirements and safety hazards. It provides a highly integrated gas supply solution for queen bee anesthesia in classrooms, enabling centralized management of gas supply through a pipeline network, thereby greatly improving safety and student experience.

[0027] The multi-channel synchronous display system includes multiple display modes such as remote online computer conferencing, operator monitors, large screens, and microscopes, which can meet the needs of various scenarios, from unified teaching and independent practice to Q&A and assessment supervision. It supports users to switch between different interfaces of trainees with one click and can accurately handle trainees' questions. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of a single system component of the artificial insemination teaching system for bees proposed in this invention;

[0029] Figure 2 This is a schematic diagram of the inter-system relationships of the honeybee artificial insemination teaching system proposed in this invention;

[0030] Figure 3 This is a schematic diagram of the innovative part of the artificial insemination teaching system for bees proposed in this invention;

[0031] Figure 4This is a schematic diagram illustrating the liquid sealing operation of the semen collection section of the artificial insemination teaching system for bees proposed in this invention.

[0032] Figure 1 In the center: 1. Local lighting - magnetic LED spotlight; 2. Operator display; 3. Global lighting - microscope internal lighting; 4. Artificial insemination instrument; 5. Carbon dioxide bubble counter; 6. Unified carbon dioxide supply pipeline; 7. Independent flow rate adjustment knob. Detailed Implementation

[0033] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0034] Please see the appendix Figure 1 -Appendix Figure 4 The artificial insemination training system for bees includes a unified carbon dioxide gas supply system, a precise cold light illumination system, a proportional silicone model, a color-marking adjustment knob, a multi-channel synchronous display system, and a precise insemination step unit. The unified carbon dioxide gas supply system includes a carbon dioxide tank, a pipeline network, a system master switch, independent switches, a carbon dioxide bubble counter, and an independent flow rate adjustment knob. The precise cold light illumination system includes a global illumination module and a local illumination module. The proportional silicone model includes proportional silicone models of drones and queen bees. The multi-channel synchronous display system includes an operator monitor, an integrator, a converter, a large monitor, and a computer for remote online conferencing. The precise insemination step unit includes a semen collection module, a queen bee processing module, and an artificial insemination module.

[0035] The semen collection module includes three steps: drone breeding, forced ejaculation, and semen collection. The queen bee treatment module includes two steps: selection of virgin queen bees and queen bee anesthesia. The artificial insemination module includes two steps: opening the stinger cavity and inserting the needle for injection.

[0036] Please see the appendix Figure 2 In a preferred embodiment, a unified carbon dioxide supply system is provided: carbon dioxide cylinders are connected to twenty or more seats via a pipeline network, the main system switch is controlled before and after the start of the class, and each student's desk is equipped with an independently designed switch, a carbon dioxide bubble meter for controlling the flow rate, and a carbon dioxide flow rate adjustment knob.

[0037] The innovative concept of a centralized gas supply system avoids the need for individual carbon dioxide cylinders for each student, saving costs and reducing the incidence of safety hazards. The unified gas supply system features a master switch for the carbon dioxide pipeline system, controlled before and after class to ensure operational safety. Furthermore, independently designed switches, carbon dioxide bubble counters for flow control, and carbon dioxide flow rate adjustment knobs allow students to make precise adjustments to the gas supply. This innovative solution not only improves operational efficiency and convenience but also significantly reduces storage requirements and safety risks, providing a highly integrated gas supply solution for queen bee anesthesia in classrooms. Centralized management of the gas supply through a pipeline network greatly improves safety and student experience.

[0038] In a preferred embodiment, the global illumination module consists of a global illumination lamp located inside the microscope, and the local illumination module consists of a magnetic LED spotlight fixed to a specific part of the microscope and externally connected. Both the global illumination lamp and the magnetic LED spotlight are low-energy cold light lamps that do not affect the activity of bee sperm.

[0039] Based on the optical principles of the microscope and its location, the internal light source, the global illumination lamp, can meet most of the lighting needs within the required operating range. In specific situations, additional supplemental lighting is required for the operating area, i.e., the local illumination module. The light source angle and intensity of this spotlight can be easily adjusted to meet specific operating needs. Trainees can activate the local illumination device at any time as needed to achieve precise supplemental lighting for the operating area.

[0040] Because bee sperm are highly sensitive to temperature, all lighting systems use low-energy cold light lamps that do not affect sperm motility, thus avoiding the impact of temperature increases on sperm activity. This lighting environment setup maximizes the protection of the experimental environment, supports microscopic operations, ensures operational precision and visibility, thereby improving the success rate of artificial insemination, and provides a suitable lighting environment for video recording of the operation process. The overall lighting system and the local lighting system work together to ensure that the most suitable lighting environment for artificial insemination operations is provided under various external environmental conditions.

[0041] In a preferred embodiment, the scaled-down silicone model is made using resin molding and 3D printing, wherein the male bee's reproductive organs and the queen bee's sting chamber are independently movable, facilitating the simulation of key operations such as sperm collection and fertilization.

[0042] The collection of sexually mature drones is greatly limited by the season, and queen bee rearing also requires a long preparation period, affecting the efficiency of promoting artificial insemination technology. To solve these practical difficulties, life-size silicone models of drones and queen bees are made. These simulated props allow training in key operations of artificial insemination to bee operations without being limited by season or cycle. The model is also suitable as a teaching model, facilitating teachers to demonstrate the use of insemination equipment, the operation of the queen bee retainer, and the adjustment of the angle and position between the queen bee, syringe, and hook. This helps trainees master the operation of each part of the artificial insemination equipment without worrying about wasting or injuring the queen bee, and also avoids the potential danger of being stung by the queen bee.

[0043] In a preferred embodiment, color-coded adjustment knobs are used to independently mark commonly used adjustment knobs, which facilitates the issuance of precise instructions by color during teaching, distinguishes different operations, and makes it easier for students to remember.

[0044] At the same time, it enables trainees to intuitively understand the structure of artificial insemination instruments, such as adjusting the corresponding parts by turning specific knobs, which facilitates long-term learning and use, familiarizes them with operation and maintenance methods, and enables them to repair the instruments themselves when problems arise after the training.

[0045] In a preferred embodiment, the operator display is a 10-inch small high-definition display, and the large display is a 120-inch high-resolution large-screen display.

[0046] Please see the appendix Figure 2 In a preferred embodiment, the multi-channel synchronous display system is as follows: each microscope is connected to an independent 10-inch small high-definition display, the display content of each microscope is integrated into a converter through an integrator, and transmitted to a 120-inch high-resolution large screen display through multi-screen projection. At the same time, remote synchronous observation and learning can be achieved with the help of computer remote online conferencing technology.

[0047] Each microscope is connected to an independent operator monitor, allowing real-time display of microscope content. This facilitates posture adjustments for trainees during operation, preventing them from being constrained by the microscope and hindering their work. It also supports mutual observation and learning. A 120-inch high-resolution large-screen monitor at the front of the classroom enables real-time content display. The multi-channel display mode, including remote online conferencing, operator monitors, large monitors, and microscopes, meets the needs of various scenarios, from unified teaching and independent practice to Q&A and assessment. It allows teachers to switch between different student interfaces with a single click and accurately address student questions. This system solves the problems of information asynchrony and difficulty in sharing under the microscope, realizing a mature information-sharing teaching platform.

[0048] In a preferred embodiment, the semen collection module includes the following specific steps:

[0049] a) Drone rearing: It takes 40 days from the queen laying drone eggs to the drones reaching sexual maturity. The health of the drones is closely related to the broodiness of the bee colony. Through proper organization of the bee colony, the queen of the paternal colony lays drone eggs on the drone comb. After 24 days, the drones emerge from their cells and are confined in drone cages and raised in the hive. The drones reach sexual maturity about 15 days after emergence. Ensure that there are enough nurse bees in the bee colony to raise healthy, sexually mature drones.

[0050] b) Forced ejaculation: Hold the male bee with your left hand and gently press its thorax. A sexually mature male bee will have a hardened and contracted abdomen. Apply pressure to the abdomen with your thumb or forefinger to force the inner penis to turn outward. Stop squeezing when a pale yellow spherical shape emerges from the ejaculation tube. The pale yellow viscous liquid outside the penis ball is the semen. Note that the pressure should be stable, and squeeze in small amounts multiple times to avoid the penis ball from rupturing or the semen from splashing out. During the operation, avoid contact between the penis ball and the semen on the surface of the male bee or your fingers to prevent semen contamination.

[0051] c) Semen Collection: Draw a short amount of saline solution into the needle to keep the needle tip moist. Secure the hook and syringe to the insemination device. When collecting semen, first draw in about 1 cm of air to separate the saline solution from the semen. Hold the drone semen close to the needle tip to avoid drawing in air. Rotate the drone with your left hand while operating the syringe knob with your right hand to draw in the semen. Collect approximately 1 μL of semen from each drone. After collecting from a single drone, perform a liquid seal in the following order: semen-air column-saline column (e.g., ...). Figure 4 As shown in the diagram, when collecting the next drone, push out the air column and saline column to continue collecting, and push out a portion of the previous drone's semen outside the needle tip, let it come into contact with the semen surface and then draw it in. After collecting the semen of 8-10 drones to form a semen column, collect the next section of the semen column by separating it into an air column-saline column-air column.

[0052] Disinfection procedures before semen collection: Wipe the pick and hook with 75% alcohol; wipe the queen bee transfer and controller (acrylic material) with medical cotton balls soaked in pure water and hydrogen peroxide; clean the injection needle twice with 75% alcohol, three times with pure water, and three times with physiological saline; fill the needle with liquid and clean it to ensure a leak-proof seal.

[0053] In a preferred embodiment, the queen bee treatment module includes the following specific steps:

[0054] a) Selection of virgin queen bees: Virgin queen bees should be cultivated and selected in advance for artificial insemination. Generally, virgin queen bees with long abdomens, steady movements, and larger size are selected. The best time for insemination is 7-10 days old.

[0055] b) Queen anesthesia: Hold the queen bee's thorax with your right hand, with the induction tube opening downwards, and insert the queen bee's head into the induction tube. After the queen bee instinctively crawls upwards into the tube, connect it to the anesthesia tube. Connect the anesthesia tube to the holder on the base of the artificial insemination device, so that 3-4 segments of the queen bee's abdomen are exposed. If the queen bee does not retreat on her own, gently tap the anesthesia tube to make it fall in, avoiding violent disturbance. Adjust the rate by adjusting the carbon dioxide flow rate knob and observe the bubble flow in the carbon dioxide bubble counter to form a stable bubble flow, preferably five bubbles per second. Anesthetize until the queen bee no longer struggles violently and begins to breathe rhythmically and tremble. The virgin queen enters a coma, and insemination can begin. The anesthesia is lifted after insemination.

[0056] While anesthetizing the queen bee, adjust the position of the insemination device so that it is in the center of the microscope's field of view. Adjust the microscope's focus and light source to ensure that the queen bee's abdomen is clearly displayed in the center of the operator's monitor. Adjust the positions of the syringe, abdominal hook, and dorsal hook on the insemination device so that it can be focused on the queen bee's position by rotation, ensuring that the abdominal hook, dorsal hook, and queen bee are in a straight line.

[0057] In a preferred embodiment, the artificial insemination module includes the following specific steps:

[0058] a) Opening the sting chamber: After the queen bee enters a comatose state, use a pry bar to open the abdominal plate, move the operating rod to hook the dorsal hook onto the abdominal plate, use the pry bar to pick out the stinger, clamp the stinger with the dorsal plate clamp, pull it open in both directions in parallel, pull the stinger towards the back, so that the distance between the abdominal plate and the dorsal plate is 3-4mm, adjust the dorsal hook to pull the stinger out of the abdominal cavity, use the double rod to pull the sting chamber open in parallel to 5-6mm, exposing the vaginal opening on the side of the stinger base near the abdomen;

[0059] b) Injection: After opening the sting chamber, fine-tune the microscope focus to clearly display the inside of the sting chamber in the center of the field of view. Observe the folded depression about 1.5mm from the sting needle in the center of the sting chamber, which is the vaginal opening. Adjust the syringe angle so that it is parallel to the queen bee's longitudinal direction and makes a 30° angle with the vertical line to contact the queen bee. After finding the vaginal opening, expel the air and saline from the tip of the insemination needle and insert the needle at a 30° angle. When resistance is felt, it indicates that the needle tip has touched the vaginal valve. Turn the control syringe knob counterclockwise until the insemination needle naturally penetrates 0.5-0.8mm, indicating successful insemination. After successfully penetrating the queen bee's pelvis, insert the needle 1mm further to ensure successful insertion. Once the insertion is confirmed, rotate the syringe control knob to inject semen. Typically, 8-10μL of semen is injected at once. If necessary, a second insemination can be performed 24-48 hours later, injecting 4-5μL of semen. After injection, withdraw the needle to release the queen bee's restriction. Once the queen bee has recovered, return her to the mating box and observe whether fertilization was successful.

[0060] If the semen level does not drop and the air column is compressed when the syringe knob is turned to inject semen, it indicates that the needle has not entered the fallopian tube and may be adsorbed on the vaginal wall or accidentally entered the lateral sac. The needle should be withdrawn immediately and the needle should be readjusted.

[0061] As can be seen from the above description, the above embodiments of the present invention achieve the following technical effects: a teaching system based on promoting artificial insemination technology for bees is constructed, the content of artificial insemination teaching for bees is improved, and it covers various aspects such as breeding bee colony organization and management, key points of insemination technology and troubleshooting.

[0062] The innovative unified carbon dioxide gas supply system not only improves operational efficiency and convenience, but also significantly reduces storage requirements and safety hazards. It provides a highly integrated gas supply solution for queen bee anesthesia in classrooms, enabling centralized management of gas supply through a pipeline network, thereby greatly improving safety and student experience.

[0063] The multi-channel synchronous display system includes multiple display modes such as remote online computer conferencing, operator monitors, large screens, and microscopes, which can meet the needs of various scenarios, from unified teaching and independent practice to Q&A and assessment supervision. It supports users to switch between different interfaces of trainees with one click and can accurately handle trainees' questions.

[0064] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention (including the claims) is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in the details for the sake of brevity.

[0065] This invention is intended to cover all such substitutions, modifications, and variations falling within the broad scope of the claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A bee artificial insemination teaching system, comprising a carbon dioxide unified gas supply system, a precise cold light illumination system, an equal proportion silica gel model, a color marking adjustment knob, a multi-channel synchronous display system and an insemination step precision unit, characterized in that, The carbon dioxide unified gas supply system includes a carbon dioxide tank, a pipeline network, a system total switch, an independent switch, a carbon dioxide bubble meter, and an independent flow rate adjustment knob, the precise cold light illumination system includes a global illumination module and a local illumination module, the equal proportion silica gel model includes an equal proportion silica gel model of drones and queen bees, the multi-channel synchronous display system includes an operator display, an integrator, a converter, a large display, and a computer remote online conference, and the insemination step precision unit includes a semen collection module, a queen bee processing module, and an artificial insemination module. The semen collection module includes three steps of drone cultivation, forced ejaculation, and semen collection, the queen bee processing module includes two steps of selection of virgin queen bees and queen bee anesthesia, and the artificial insemination module includes two steps of opening the sting cavity and needle injection.

2. The honeybee artificial insemination teaching system according to claim 1, characterized in that, The carbon dioxide unified gas supply system: the carbon dioxide tank is connected to twenty or more seats through the pipeline network, the system total switch is controlled before and after the teaching is started, and each student's desktop is equipped with an independently designed switch, a carbon dioxide bubble meter for controlling the flow rate, and a carbon dioxide flow rate adjustment knob.

3. The bee artificial insemination teaching system according to claim 1, characterized in that, The global illumination module is composed of a global illumination lamp located inside the microscope, the local illumination module is composed of a magnetically attracted LED spotlight fixed at a specific position of the microscope and externally connected, and the global illumination lamp and the magnetically attracted LED spotlight both adopt low-energy cold light lamps that do not affect the activity of bee sperm.

4. The bee artificial insemination teaching system according to claim 1, characterized in that, The equal proportion silica gel model is made by resin reverse modeling and 3D printing, wherein the reproductive organs of drones and the sting cavity of queen bees are independently movable.

5. The bee artificial insemination teaching system according to claim 1, characterized in that, The color marking adjustment knob independently marks the commonly used adjustment knob with different colors, which can issue precise instructions through colors during teaching, distinguish different operations, and facilitate students' memory.

6. The honeybee artificial insemination teaching system of claim 1, wherein, The operator display adopts a 10-inch small high-definition display, and the large display adopts a 120-inch high-resolution large-screen display.

7. The honeybee artificial insemination teaching system according to claim 6, characterized in that, The multi-channel synchronous display system: each microscope is connected with an independent 10-inch small high-definition display, the display content of each microscope is integrated on a converter through an integrator, and is transmitted to a 120-inch high-resolution large-screen display through multi-screen projection, and remote synchronous observation and learning can be realized with the help of computer remote online conference technology.

8. The honeybee artificial insemination teaching system of claim 1, wherein, The semen collection module includes the following specific steps: a) Drone cultivation: through reasonable organization of the bee colony, the queen bees of the father colony produce drone eggs on the drone nest, and the drones emerge from the house after 24 days, are imprisoned in the drone cage, and are cultivated in the beehive. The drones reach sexual maturity about 15 days after emergence, and sufficient nurse bees are ensured in the bee colony to cultivate healthy sexually mature drones; b) Forced ejaculation: the left hand holds the drone, the chest is lightly pressed, the abdomen is contracted and hard, it is a sexually mature drone, the thumb or index finger is pressed on the abdomen, the internal penis is forced to evert, when the ejaculatory duct emerges as a pale yellow ball, stop pressing, the pale yellow viscous liquid on the penis ball is semen, pay attention to stable pressure, a small amount of multiple extrusion, avoid the penis ball and semen from contacting the drone body surface or fingers, and prevent the semen from being contaminated; c) semen collection: in the needle suction physiological saline, keep the needle tip wet, hook and syringe fixed to the insemination instrument, semen collection, first suction about 1 cm air, physiological saline and semen separated, the male bee semen close to the needle tip, avoid suction air, left hand turn male bee, right hand operation syringe knob suction, each male bee about 1 μL semen, single male bee collection, according to the order of "semen-air column-physiological saline column" liquid seal, collection of the next male bee, push out the air column and physiological saline column continue to collect, and the previous male bee semen push out a part of the needle tip, contact with the semen surface after suction, semen column after collection of 8-10 male bees, through the air column-physiological saline column-air column separation of the lower part of the semen column collection.

9. The bee artificial insemination teaching system of claim 1, wherein, The queen bee processing module comprises the following specific steps: a) selection of virgin queen: artificial insemination should be carried out in advance to cultivate and screen virgin queen, virgin queen generally selects abdomen large, action steady, individual larger, the best insemination period is 7-10 days old; b) queen anesthesia: right hand hold the queen chest, the induction pipe opening downward, the queen head into the induction pipe, after the queen instinct to climb up, with the anesthesia pipe docking, the anesthesia pipe connected to the artificial insemination instrument base on the fixator, so that the queen abdomen exposed 3-4 segments, if the queen does not fall into the light knock anesthesia pipe, avoid violent disturbance, through the carbon dioxide flow rate adjustment knob to adjust the rate, observe the carbon dioxide meter bubble ware bubble flow, form stable bubble flow, five bubbles per second is appropriate; anesthesia to the queen no longer struggle, start rhythm breathing shake, virgin queen into coma state, can start insemination, after the end of insemination, remove anesthesia.

10. The bee artificial insemination teaching system of claim 1, wherein, The artificial insemination module comprises the following specific steps: a) open the sting chamber: when the queen enters the coma state, use the pick to open the abdominal plate, move the operating rod to hook the back hook to the abdominal plate, use the pick to pick out the sting, use the back plate clamp to clamp the sting, pull the sting to the back, make the abdominal plate and back plate spread apart 3-4 mm, adjust the back hook, pull the sting out of the abdominal cavity, use the double rod to pull the sting chamber parallel to 5-6 mm, expose the vaginal orifice on the side of the abdomen near the base of the sting; b) needle injection: after the opening of the needle cavity, fine-tune the microscope focus, so that the needle cavity is clearly presented in the central field of view, observe the fold depression about 1.5 mm from the needle in the central cavity, which is the vaginal orifice, adjust the angle of the syringe so that it is parallel to the longitudinal direction of the queen bee, and the angle with the vertical line is 30°, contact the queen bee, find the vaginal orifice, exclude the air and physiological saline at the tip of the insemination needle, and insert the needle at an angle of 30°; when resistance is felt, it indicates that the needle tip touches the vaginal flap, the control knob of the syringe needs to be counterclockwise rotated, and when the insemination needle naturally penetrates 0.5-0.8 mm, it indicates that it has successfully bypassed the flap, and the needle is inserted again 1 mm, which means successful insertion; after confirming the correct insertion, rotate the control knob of the syringe to inject semen, usually 8-10 μL of semen is injected at a time, and according to the need, secondary insemination can be performed after 24-48 hours, injecting 4-5 μL of semen, after injection, the needle is withdrawn, the queen bee is released, and after the queen bee wakes up, it is sent back to the mating box, and whether the insemination is successful is observed; 4-5 μL of semen is injected, after injection, the needle is withdrawn, the queen bee is released, and after the queen bee wakes up, it is sent back to the mating box, and whether the insemination is successful is observed.

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