Compound Probiotic Solid Beverage for Weight Loss and Lipid Reduction and Its Preparation Process

Abstract

The present invention provides a weight-loss and lipid-lowering compound probiotic solid beverage and a preparation process thereof, belonging to the field of solid beverages. Specifically, a compound probiotic solid beverage is provided, comprising: compound probiotic powder, collagen peptide, L-arabinose, barley grass powder and ascorbic acid; through model verification, the solid beverage of the present invention can achieve the effects of lipid-lowering and weight loss.

Description

Technical Field

[0001] The invention belongs to the field of solid beverages, and in particular relates to a weight-loss and lipid-lowering composite probiotic solid beverage and a preparation process thereof. Background Art

[0002] Probiotics refer to live bacteria preparations and their metabolites that improve the host's microecological balance and play a beneficial role, thereby improving the host's health level and health status. The beneficial bacteria or fungi in animals mainly include: lactic acid bacteria, bifidobacteria, actinomycetes, yeast, etc. The most powerful products currently being studied are mainly composite probiotics composed of the above types of microorganisms.

[0003] There are many forms of compound probiotic products, such as capsules containing probiotics, freeze-dried powder, microcapsule preparations, etc. Solid beverages refer to solid products made from sugar, milk and dairy products, eggs or egg products, fruit juice or edible plant extracts as the main raw materials, with appropriate amounts of auxiliary materials or food additives added, and the moisture content of each 100 grams of finished product is not more than 5 grams. They are in powder, granular or block form. Solid beverages are made by removing water from liquid beverages. The purpose of removing water is: first, to prevent the dried beverage from deteriorating or corrupting due to its own enzymes or microorganisms, so as to facilitate storage; second, to facilitate storage and transportation. Compared with liquid beverages, solid beverages have the following characteristics: significantly reduced weight, significantly smaller volume, and easy to carry; good flavor, good solubility, wide range of applications, and easy drinking; easy to maintain hygiene; simple packaging and convenient transportation.

[0004] Probiotics for weight loss and lipid reduction have been disclosed in the prior art. Using probiotics for weight loss and lipid reduction is safer. The preparation of solid beverages with weight loss and lipid reduction effects by composite probiotics can make the application of probiotics more extensive and convenient.

[0005] A dietary fiber probiotic solid beverage is disclosed in the Chinese patent with application number CN202110820550.8, including the following raw materials: composite probiotics, maltodextrin powder, dietary fiber, fruit and vegetable enzymes, whole milk powder, and food additives; the composite probiotics include Lactobacillus plantarum, Bifidobacterium longum, Pediococcus lactis, Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus rhamnosus, Bifidobacterium breve, Bifidobacterium lactis, and Lactobacillus fermentum; by adding a variety of probiotics to solid beverages, a beneficial interaction occurs between a variety of probiotics, effectively increasing the diversity of intestinal flora; by adding dietary fiber to solid beverages, it is beneficial to play a good regulatory role on probiotics in the human body; by adding fruit and vegetable enzymes to solid beverages, it has the effect of regulating the intestinal microecology of the human body, laxative, and weight loss; by adding whole milk powder and food additives to solid beverages, not only the nutritional value of solid beverages can be improved, but also the taste of solid beverages can be effectively improved. However, its ingredients are relatively complex, and it is inconvenient to use it for industrialization.

[0006] Developing probiotic solid beverages with simple ingredients, suitable for large-scale production while ensuring weight loss and lipid-lowering effects is the development trend of the industry. Summary of the Invention

[0007] To solve the above problems, the present invention provides a weight loss and lipid-lowering compound probiotic solid beverage and its preparation process.

[0008] On the one hand, the present invention provides a compound probiotic solid beverage.

[0009] The compound probiotic solid beverage includes: compound probiotic powder, collagen peptide, L-arabinose, barley grass powder, and ascorbic acid;

[0010] The compound probiotic powder includes Lactobacillus fermentum, Bacillus coagulans, and Lactobacillus plantarum, and the ratio of bacteria content is 2-3:2-3:2-3.

[0011] Preferably, by weight, it includes: 10-15 parts of Lactobacillus fermentum, 10-15 parts of Bacillus coagulans, 10-15 parts of Lactobacillus plantarum, 5-7 parts of collagen peptide, 4-6 parts of L-arabinose, 11-16 parts of barley grass powder, and 0.2-0.4 parts of ascorbic acid.

[0012] More preferably, by weight, it includes: 15 parts of Lactobacillus fermentum, 15 parts of Bacillus coagulans, 10 parts of Lactobacillus plantarum, 7 parts of collagen peptide, 6 parts of L-arabinose, 11.8 parts of barley grass powder, and 0.2 parts of ascorbic acid.

[0013] Optionally, the Lactobacillus fermentum, Bacillus coagulans, and Lactobacillus plantarum in the compound probiotic powder are viable bacteria or inactivated bacteria.

[0014] Preferably, the compound probiotic powder is obtained by mixing Lactobacillus fermentum powder, Bacillus coagulans powder, and Lactobacillus plantarum powder, and the bacteria content in the Lactobacillus fermentum powder, Bacillus coagulans powder, and Lactobacillus plantarum powder is 10 10 -10 11 CFU / g.

[0015] More preferably, the bacteria content in the Lactobacillus fermentum powder, Bacillus coagulans powder, and Lactobacillus plantarum powder is 5×10 10 -1×10 11 CFU / g.

[0016] In an alternative technical solution, the preparation method of the barley grass powder is: take young barley leaves, freeze-dry them and then perform air pulverization to obtain it.

[0017] On the other hand, the present invention provides a method for preparing the aforementioned compound probiotic solid beverage.

[0018] The preparation method includes: weighing the corresponding raw materials according to the formula and mixing them evenly to obtain the product.

[0019] Preferably, the preparation method includes:

[0020] Weigh the raw materials according to the formula amount, add an ethanol aqueous solution of 10wt%-30wt%, mix and stir for 10-30 min, and dry at 40-60 °C. It is required that the moisture content of the obtained product is lower than 3%.

[0021] In some specific embodiments, the ethanol aqueous solution is a 20wt% ethanol aqueous solution; the mixing and stirring time is 15 min; the drying temperature is 50 °C; the moisture content of the product is lower than 2%.

[0022] On yet another aspect, the present invention provides the application of the aforementioned compound probiotic solid beverage in the preparation of lipid-lowering and weight-loss products.

[0023] The product can be a product derived based on the technical solution of the present invention, such as oral liquid, beverage, etc. Although the present invention provides a solid beverage, in fact, various forms of products can be prepared through the formula of this solid beverage to achieve the purpose of weight loss and lipid reduction. These downstream products are also within the protection scope of the present invention.

[0024] Advantages of the present invention:

[0025] The compound probiotics and other components in the present invention can play a synergistic and enhancing effect. Compared with the prior art, the weight loss effect and lipid reduction effect on the model are significantly improved. Moreover, the raw materials used in the present invention are easily available, harmless to the human body, the product has a suitable taste, and has good production and investment prospects. Description of the Drawings

[0026] Figure 1 It is the relative change rate of the body weight of mice in the effect experimental example. Detailed Embodiments

[0027] The following combines specific embodiments to further elaborate on the present invention. The following embodiments are not used to limit the present invention, but only to illustrate the present invention. The experimental methods used in the following embodiments, unless otherwise specified, and the experimental methods without specific conditions noted in the embodiments are usually carried out under conventional conditions. The materials, reagents, etc. used in the following embodiments, unless otherwise specified, can be obtained from commercial channels.

[0028] The public information on the sources of some raw materials used in the embodiments is as follows:

[0029] Probiotic 1: Lactobacillus fermentum, with the deposit number of CGMCC No. 14493;

[0030] Probiotic 2: Bacillus coagulans, with the deposit number of CCTCC NO: M2017813;

[0031] Probiotic 3: Lactobacillus plantarum, with the deposit number of CCTCC No. 2019084.

[0032] Probiotics 1 - 3 are all strains of prior art. Among them, probiotics 1 - 2 are disclosed in the existing published patent CN202210468076.1, and probiotic 3 is disclosed in the existing published patent CN202210468076.1.

[0033] Collagen peptide is purchased from: Zhongshi Duqing (Shandong) Biotechnology Co., Ltd.

[0034] Examples 1 - 3

[0035] The formula of the weight - loss and lipid - lowering compound probiotic solid beverage is shown in Table 1:

[0036] Table 1 Formula of the weight - loss and lipid - lowering compound probiotic solid beverage (parts by weight)

[0037] Component Example 1 Example 2 Example 3 Probiotic 1 (Lactobacillus fermentum) 15 15 10 Probiotic 2 (Bacillus coagulans) 10 15 15 Probiotic 3 (Lactobacillus plantarum) 15 10 15 Collagen peptide 5 7 6 L-Arabinose 4 6 5 Barley seedling powder 15.8 11.8 13.6 Ascorbic acid 0.2 0.2 0.4

[0038] In the above formula, probiotics 1 - 3 are provided in the form of live bacterial powders. The preparation method of the bacterial powder refers to the prior art and specifically may include:

[0039] First, the preserved probiotics are respectively activated in MRS liquid medium (Thermo Fisher) at 37 °C for 18 h to obtain activated bacterial liquid. The activated bacterial liquid is inoculated into MRS liquid medium at an inoculation amount of 1% - 5%, and anaerobically cultured at 37 °C for 24 h to obtain fermentation broth; the fermentation broth is centrifuged at 10000 rpm for 20 min respectively, the bacterial cells are collected and freeze - dried to obtain bacterial powders respectively. The content of each probiotic in the bacterial powder of this example is 5×10 10 CFU / g.

[0040] The preparation method of barley grass powder is: Take young barley leaves, freeze - dry them and then perform air - flow pulverization to obtain it.

[0041] The preparation method of the solid beverage: Weigh the corresponding raw materials according to the formula and the weight of the target product, and mix them evenly to obtain it.

[0042] Example 4

[0043] Referring to the formula of Example 2, each probiotic is replaced with an inactivated bacterial powder.

[0044] Example 5

[0045] Referring to the formula of Example 2, the content of each probiotic in the bacterial powder is 1×10 11 CFU / g.

[0046] Example 6

[0047] Referring to the formula of Example 2, the preparation method of the solid beverage is as follows: Weigh the raw materials according to the formula, add an equal mass of 20wt% ethanol aqueous solution, mix and stir for 15 min, and dry at 50°C. It is required that the water content of the obtained product is less than 2%.

[0048] Comparative Example 1

[0049] Referring to Example 2, all probiotic components are replaced with: 20 parts of probiotic 1 + 20 parts of probiotic 2.

[0050] Comparative Example 2

[0051] Referring to Example 2, all probiotic components are replaced with: 40 parts of probiotic 3.

[0052] Comparative Example 3

[0053] Referring to Example 2, all probiotic components are replaced with: 40 parts of probiotic 1.

[0054] Comparative Example 4

[0055] Referring to Example 2, the difference is that it does not contain probiotics.

[0056] Comparative Example 5

[0057] Referring to Example 2, the difference is that it only includes probiotics.

[0058] Comparative Example 6

[0059] Referring to Example 2, the barley grass powder is replaced with kale powder.

[0060] Effect Experimental Example Verification of the Effect of the High-Fat Mouse Model

[0061] SPF-grade C57BL / 6N male mice (6 weeks old, body weight 20±2 g) were randomly assigned to each group, with 10 mice in each group. According to the corresponding treatment methods, they were divided into 3 groups: blank control group, model group, and experimental group (Examples and Comparative Examples), as follows:

[0062] Blank control group: Fed with normal feed and gavaged with 10 mL of water daily;

[0063] Model group: Fed with high-fat feed and gavaged with 10 mL of water daily;

[0064] Experimental group: Fed with high-fat feed and the solid beverage prepared according to Examples and Comparative Examples;

[0065] 60% of the energy in high-fat feed came from fat and was purchased from Beijing Keao Xieli Feed Co., Ltd.

[0066] Examples 1-5 and Comparative Examples 1-3 and 6 were dissolved in 10 mL of water at 1 g and administered intragastrically once a day; Comparative Example 4 was dissolved in 10 mL of water according to the amount of viable bacteria in Example 2 and administered intragastrically once a day; Comparative Example 5 was dissolved in 10 mL of water according to the amount of non-viable bacteria components (collagen peptide, L-arabinose, barley grass powder, ascorbic acid) in Example 2 and administered intragastrically once a day.

[0067] The mice were allowed to eat and drink freely and their body weights were measured once a week.

[0068] The experimental period was 6 weeks, and blood was collected from the eyeballs on the last day. Fasting for 12 hours before sacrifice, and the liver was taken after sacrifice. Total cholesterol (TC, ELISA ml037202), triglycerides (TG, ELISA ml095894), high-density lipoprotein (HDL, ELISA ml037767), low-density lipoprotein (LDL, ELISA ml063218), alanine aminotransferase (ALT, ELISA ml058648), and aspartate aminotransferase (AST, ELISA ml058659) in mouse serum and liver were detected using commercially available kits. The data obtained eliminated outliers to ensure that each group of data results was not less than 7, and the statistical results were:

[0069] (1) Relative rate of change in body weight

[0070] Relative change rate of body weight = (X1-X1') / X1'×100%;

[0071] Where X1' is the weight change rate of the model group, X1' = Y-Y', Y is the last average weight of the model group, and Y' is the initial average weight of the model group;

[0072] X1 is the weight change rate of the blank control group or the experimental group, X1=Z-Z', Z is the last average weight of the blank control group or the experimental group, and Z' is the first average weight of the blank control group or the experimental group.

[0073] That is, the relative change rate of body weight reflects the trend of body weight change in the model group or control group relative to the blank control group.

[0074] Results Figure 1 The specific result data are as follows:

[0075] Relative change rate of body weight (%) Blank control -8.68％ Model 0 Example 1 -6.74％ Example 2 -8.25％ Example 3 -6.93％ Example 4 -6.17％ Example 5 -8.79％ Example 6 -8.14％ Comparative example 1 -2.84％ Comparative example 2 -1.65％ Comparative example 3 -0.78％ Comparative example 4 -0.31％ Comparative example 5 -2.66％ Comparative example 6 -5.16％

[0076] Compared with the blank control group, the body weight of the model group increased significantly, indicating that the model was successfully constructed; the average body weight of the mice in Examples 1-6 was lower than that of the model group, indicating that the solid beverage products prepared in the examples had a good weight loss effect. By comparing the effects of Comparative Examples 1-3 with those of Example 2, the synergistic effect among various bacteria could be seen. Specifically, the weight loss effect of the compound bacteria was significantly better than that of the single bacteria under the same bacterial content condition, and the three-bacteria combination was better than the two-bacteria combination. From the results of Comparative Examples 4-6, it could be seen that there was a good combined effect among the non-viable bacterial components of the solid beverage, and it could exert a synergistic effect with the compound bacteria, further enhancing the weight loss effect.

[0077] (2) The results of blood indexes are shown in Table 2 below:

[0078] Table 2

[0079]

[0080] (3) The results of liver indexes are shown in Table 3 below:

[0081] Table 3

[0082]

[0083]

[0084] The test results in blood indexes and liver indexes indicate that the compound probiotic solid beverage of the present invention has a positive effect on the relevant markers in blood and liver, corresponding to the body weight change.

[0085] It should be noted that the above examples are only specific embodiments of the technical solution of the present invention, used to illustrate the technical solution of the present invention, and do not limit the protection scope of the present invention. Replacements and optimizations of conventional means made by those skilled in the art based on the technical solution of the present invention are also within the protection scope of the present invention. Technical solutions of further adding common excipients in the fields of food or health products or drugs to facilitate better application on the basis of the solid beverage formula provided by the present invention are also within the protection scope of the present invention.

Claims

1. A compound probiotic solid beverage, characterized in that, It consists of the following components by weight: 10 - 15 parts of Lactobacillus fermentum, 10 - 15 parts of Bacillus coagulans, 10 - 15 parts of Lactobacillus plantarum, 5 - 7 parts of collagen peptide, 4 - 6 parts of L - arabinose, 11 - 16 parts of barley seedling powder, and 0.2 - 0.4 parts of ascorbic acid; the preservation number of the Lactobacillus fermentum is CGMCC No.14493, the preservation number of the Bacillus coagulans is CCTCC NO: M2017813, and the preservation number of the Lactobacillus plantarum is CCTCC No.2019084.

2. The compound probiotic solid beverage according to claim 1, wherein It consists of the following components by weight: 15 parts of Lactobacillus fermentum, 15 parts of Bacillus coagulans, 10 parts of Lactobacillus plantarum, 7 parts of collagen peptide, 6 parts of L - arabinose, 11.8 parts of barley seedling powder, and 0.2 parts of ascorbic acid.

3. The compound probiotic solid beverage according to claim 2, wherein The Lactobacillus fermentum, Bacillus coagulans, and Lactobacillus plantarum are viable bacteria or inactivated bacteria.

4. The compound probiotic solid beverage according to claim 3, wherein The bacterial contents in the Lactobacillus fermentum powder, Bacillus coagulans powder and Lactobacillus plantarum powder are 10 10 -10 11 CFU / g, respectively.

5. The compound probiotic solid beverage according to claim 4, characterized in that, The bacterial contents in the Lactobacillus fermentum powder, Bacillus coagulans powder and Lactobacillus plantarum powder are 5×10 10 -1×10 11 CFU / g respectively.

6. The compound probiotic solid beverage according to claim 2, wherein The preparation method of the barley seedling powder is as follows: Take young barley leaves, freeze - dry them and then perform air - flow pulverization to obtain it.

7. The preparation method of the compound probiotic solid beverage according to any one of claims 1-6, characterized in that, It includes: Weigh the corresponding raw materials according to the formula and mix them evenly to obtain it.

8. The preparation method of the compound probiotic solid beverage according to any one of claims 1-6, characterized in that, It includes weighing the raw materials according to the formula amount, adding an equal mass of 20wt% ethanol aqueous solution, mixing and stirring for 15 min, and drying at 50 °C, requiring the water content of the obtained product to be less than 2%.

9. Use of the compound probiotic solid beverage according to any one of claims 1 - 6 in the preparation of lipid - reducing and weight - losing products.

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