A bio-detection chip and a detection method thereof

Abstract

This invention discloses a biochip and its detection method. The chip includes: a chip substrate, a glass slide base plate fitted outside the chip substrate, a glass slide cover plate installed above the glass slide plate, a plurality of nylon membranes arranged on the chip substrate, and molecular microarrays arranged on each of the plurality of nylon membranes. One end of the chip substrate is connected to a fixing plate, which is fixedly inserted into the glass slide base plate. The glass slide cover plate has a plurality of liquid inlet chambers, and liquid inlet heads are respectively installed on the liquid inlet ends of the plurality of liquid inlet chambers. A needle is respectively installed at the front end of the plurality of liquid inlet heads. This invention relates to the field of biochip technology and has the functions of extracting test liquid and chip detection, which are combined into one. It is easy to carry and easy to aseptically package. In use, pushing and pulling the plate can realize a series of processes such as extracting test liquid, delivering test liquid onto the biochip, and biochip detection.

Description

Technical Field

[0001] This invention relates to the field of biochip technology, specifically to a biodetection chip and its detection method. Background Technology

[0002] Simply put, a biochip involves placing a biological sample on a small slide, such as a glass slide, silicon wafer, or nylon membrane, about the size of a fingernail. An instrument then collects the signals, and a computer analyzes the data. Currently, solid-phase materials used to prepare biochips include glass slides, silicon wafers, metal sheets, and nylon membranes. Glass slides are the most commonly used support material because they are suitable for various synthesis methods, and the pretreatment of glass slides before fabrication is relatively simple and easy.

[0003] The use of biochips is quite simple: the solution to be tested is brought into contact with probes on the biochip, and the test result is obtained. However, in existing technologies, the biochip and the extraction equipment for the solution to be tested are separate, meaning that the test results are obtained by using two devices together. For example, invention patent CN107418874A discloses a biochip belonging to the field of biodetection technology, which includes: a base, several chip handles mounted on the base in an array, the chip handles extending parallel to each other from the base in a direction perpendicular to the base, and chip reaction interfaces for marking the test probes arranged at the free ends of each chip handle. The longitudinal arrangement of the chip reaction interfaces coincides with the longitudinal direction of the chip handles. This invention's biochip is simple and practical, belonging to traditional biochips. It needs to be used in conjunction with existing equipment for the solution to be tested. This method is suitable for use in a clean and sterile indoor environment such as a laboratory, but it is not suitable for use outdoors. In addition, the use of two devices together can lead to contamination such as mixed bacteria. Therefore, there are high requirements and precautions when using it. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a biological detection chip and its detection method, solving the problem of using existing biological chips in conjunction with test liquid extraction equipment.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: a biological detection chip, the chip comprising: a chip substrate, a glass slide base plate fitted outside the chip substrate, a glass slide cover plate installed above the glass slide base plate, a plurality of nylon membranes arranged on the chip substrate, a molecular microarray arranged on each of the plurality of nylon membranes, a fixing plate connected to one end of the chip substrate, the fixing plate being fixedly inserted into the glass slide base plate;

[0006] The glass slide cover plate is provided with a plurality of liquid inlet chambers, and a liquid inlet head is installed on the liquid inlet end of each of the plurality of liquid inlet chambers. A needle is installed at the front end of each of the plurality of liquid inlet heads. A one-way check mechanism is provided in each of the plurality of liquid inlet heads. A piston is movably installed in each of the plurality of liquid inlet chambers. A pull rod is fixedly connected to one end of each of the plurality of pistons. A sampling mechanism is installed at one end of each of the plurality of pull rods.

[0007] The glass slide base plate is provided with a plurality of medicine tanks, and each of the medicine tanks is provided with a liquid guiding mechanism.

[0008] The liquid guiding mechanism includes an inlet tube and several outlet tubes formed inside the glass slide base plate. The upper end of the inlet tube is provided with a leakage port at the connection between it and the liquid inlet chamber, and the lower end of the outlet tube is located above the nylon membrane.

[0009] Preferably, the one-way check mechanism includes an inlet groove and an outlet groove formed inside the inlet head, a guide groove connecting the inlet groove and the outlet groove, a cavity provided inside the inlet head, a plunger movably installed in the cavity, the two ends of the plunger being movably inserted into the inlet groove and the outlet groove respectively and having an inlet channel and an outlet channel respectively, a limit plate fixedly fitted outside the plunger, and a spring fitted outside the plunger, the two ends of the spring contacting the limit plate and the inner wall of the cavity respectively.

[0010] Preferably, the sampling mechanism includes a piston rod fixedly connected to one end of the pull rod, an annular groove is formed in the side wall of one end of the piston rod, a groove is formed in the glass slide cover plate, one end of the piston rod slides in the groove, a pull plate is provided on one side of the glass slide cover plate, a slot is formed in the inside of one side of the pull plate, the slot is fitted onto the outside of the other end of the piston rod, and a fixing mechanism is provided in the pull plate.

[0011] Preferably, the upper and lower ends of the inlet pipe are connected to the upper wall of the liquid inlet chamber and the medicine tank, respectively, and the upper ends of several outlet pipes are connected to the lower wall of the medicine tank. The inlet pipe is provided with a one-way permeation membrane mechanism.

[0012] Preferably, the fixing mechanism includes a first slide groove formed in the pull plate, a slide rod slidably inserted in the first slide groove, one end of the slide rod extending into the outside of the pull plate and the other end fixedly connected to a second spring, one end of the second spring being connected to the inner wall of the first slide groove, a second slide groove formed in the side wall of the first slide groove, a third slide groove formed in one side wall of the second slide groove, the third slide groove communicating with the slot, a connecting rod slidably disposed in the second slide groove, one end of the connecting rod being connected to the slide rod and the other end being connected to a plug on the side wall, the plug sliding in the third slide groove and one end being inserted into the annular slide groove.

[0013] Preferably, the one-way permeation membrane mechanism includes an elastic resin membrane fixedly installed inside the inlet tube. The elastic resin membrane has a plurality of through holes, each through hole including an upper opening and a lower opening. A connection port is connected between the upper opening and the lower opening. The diameter of the lower opening is smaller than the diameter of the upper opening but larger than the diameter of the connection port.

[0014] Preferably, the pull plate has inserts at both ends, the glass cover plate has a pair of slots inside, the pair of inserts are inserted into the pair of slots, the inner walls of the inserts on both sides are provided with arc-shaped grooves, the inner walls of the slots on both sides are provided with grooves, ball bearings are movably disposed in the pair of grooves, the pair of ball bearings are inserted into the pair of arc-shaped grooves, the pair of ball bearings are connected to a third spring, and the pair of third springs are connected to the inner walls of the grooves.

[0015] A detection method for a bio-detection chip, the detection method comprising:

[0016] S1: First, attach the nylon membrane carrying the biochemical reaction organism to the chip substrate. Then, insert the chip substrate into the glass slide base plate. When attaching the nylon membrane, ensure the spacing between them so that the outlet of each tube will correspond to the nylon membrane after insertion.

[0017] S2: Then insert the syringe into the solution to be tested, and pull the pull plate outward. The pull plate can drive the piston column to move outward. The piston column drives the piston to move through the pull rod. Under pressure, the solution to be tested will enter the inlet tank from the syringe and enter the inlet chamber through the outlet tank along the guide tank.

[0018] S3: Then push the pull plate, and the solution to be tested will enter the tank through the inlet pipe from the leak, and finally flow out from the outlet pipe to contact the nylon membrane below, completing the testing process.

[0019] Preferably, when the piston moves inward along the inlet chamber, the elastic resin diaphragm bulges upward due to air pressure from below the inlet pipe. At this time, the lower opening at the bottom of the through hole closes under the elastic action of the elastic resin diaphragm, keeping the entire through hole closed. When the piston moves outward along the inlet chamber, the elastic resin diaphragm is subjected to downward pressure from the test solution above the inlet pipe, causing the elastic resin diaphragm to concave downward. The lower opening opens at this time. Since the opening diameter of the upper opening is larger than that of the lower opening, the upper opening does not close when the elastic resin diaphragm concave downward, giving the elastic resin diaphragm a unidirectional conduction effect.

[0020] Beneficial effects

[0021] This invention provides a biological detection chip and its detection method, which have the following beneficial effects:

[0022] The present invention provides a biodetection chip that integrates the functions of sample extraction and chip detection into one, making it easy to carry and package aseptically. In use, the process of extracting the sample, delivering the sample onto the biochip, and performing biochip detection can be achieved simply by pushing and pulling the plate. It is simple and easy to use and is not limited by external conditions.

[0023] An elastic resin diaphragm is installed inside the inlet pipe, which has a one-way conduction function. The diameter of the upper opening inside the through hole is larger than the diameter of the lower opening, and both of them have a conical structure. When the elastic resin diaphragm undergoes elastic deformation, when it bulges upward, the lower opening will close, and when it bulges downward, the lower opening will open, while the upper opening will not close, thus achieving a one-way conduction function. Attached Figure Description

[0024] Figure 1 This is a top sectional view of the present invention.

[0025] Figure 2 This is a top view of the glass slide base plate of the present invention.

[0026] Figure 3 This is a partially enlarged cross-sectional view of the liquid inlet head of the present invention.

[0027] Figure 4 This is a partially enlarged schematic diagram of the piston column of the present invention.

[0028] Figure 5 This is a schematic diagram of the structure of the medicine tank of the present invention.

[0029] Figure 6 This is a schematic diagram of the structure of the elastic resin film of the present invention.

[0030] Figure 7 This is a partial top-view enlarged schematic diagram of the chip substrate of the present invention.

[0031] In the diagram: 1. Slide cover plate; 2. Inlet head; 3. Needle; 4. Inlet chamber; 5. Pull rod; 6. Drain outlet; 7. Piston; 8. Insert; 9. Insert block; 10. Ball bearing; 11. Pull plate; 12. Second spring; 13. Slide rod; 14. Piston column; 15. Annular groove; 16. Plug; 17. Connecting rod; 18. Slide base plate; 19. Inlet tube; 20. Medicine tank; 21. Chip substrate; 22. Fixing plate; 23. Outlet tube; 24. Nylon membrane; 25. Inlet groove; 26. Plunger; 27. Inlet channel; 28. Guide groove; 29. ​​Limiting plate; 30. Spring; 31. Outlet channel; 32. Outlet groove; 33. Elastic resin membrane; 34. Through hole; 35. Top opening; 36. Bottom opening; 37. Connection port. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] Please see Figure 1-7 The present invention provides a technical solution: a biological detection chip, the chip comprising: a chip substrate 21, a glass slide base plate 18 fitted on the outside of the chip substrate 21, a glass slide cover plate 1 installed above the glass slide base plate 18, a plurality of nylon films 24 arranged on the chip substrate 21, each of the plurality of nylon films 24 being arranged with a molecular microarray, and a fixing plate 22 connected to one end of the chip substrate 21, the fixing plate 22 being fixedly inserted into the glass slide base plate 18;

[0034] The nylon membrane 24 on the chip substrate 21 is arranged in the same way as the outlet tube 23, and the outlet of the nylon membrane 24 is close to the outlet of the outlet tube 23, which can ensure that the liquid to be tested coming out of the outlet tube 23 comes into contact with the nylon membrane 24 in a timely manner. In this design, the chip substrate 21 will have several small square grooves corresponding to the position of the outlet tube 23, which facilitates the placement of the nylon membrane 24 and ensures that the liquid to be tested falls into the square groove and comes into contact with the nylon membrane 24 quickly.

[0035] The glass slide cover plate 1 is provided with a plurality of liquid inlet chambers 4, and a liquid inlet head 2 is respectively installed on the liquid inlet end of the plurality of liquid inlet chambers 4. A needle tube 3 is respectively installed at the front end of the plurality of liquid inlet heads 2. A one-way check mechanism is respectively provided in the plurality of liquid inlet heads 2. A piston 7 is movably installed in the plurality of liquid inlet chambers 4. A pull rod 5 is fixedly connected to one end of the plurality of pistons 7. A sampling mechanism is respectively installed at one end of the plurality of pull rods 5.

[0036] The design here uses the push-pull rod 5 to drive the piston 7 to move in and out of the liquid inlet chamber 4, thereby realizing a series of actions to draw the test liquid from the syringe 3 and discharge the test liquid from the leak port 6.

[0037] The slide base plate 18 is provided with a plurality of medicine tanks 20, and each of the medicine tanks 20 is provided with a liquid guiding mechanism.

[0038] The liquid guiding mechanism includes an inlet pipe 19 and several outlet pipes 23 formed in the glass slide base plate 18. The upper end of the inlet pipe 19 is provided with a leakage port 6 at the connection between it and the liquid inlet chamber 4. The lower end of the outlet pipe 23 is located above the nylon membrane 24.

[0039] Furthermore, the one-way check mechanism includes an inlet groove 25 and an outlet groove 32 formed inside the inlet head 2. A guide groove 28 is connected between the inlet groove 25 and the outlet groove 32. The inlet head 2 has a cavity, and a plunger 26 is movably installed in the cavity. The two ends of the plunger 26 are respectively movably inserted into the inlet groove 25 and the outlet groove 32 and respectively have an inlet channel 27 and an outlet channel 31. A limiting plate 29 is fixedly fitted on the outside of the plunger 26, and a spring 30 is fitted on the outside of the plunger 26. The two ends of the spring 30 are in contact with the limiting plate 29 and the inner wall of the cavity, respectively.

[0040] When the liquid to be tested is drawn, pulling the pull plate 11 will eventually pull the piston 7, and the plunger 26 will move towards the liquid outlet 32. At this time, the spring 30 will be compressed, and the liquid inlet 25, liquid inlet channel 27, liquid guide channel 28, liquid outlet channel 31 and liquid outlet 32 ​​will form a passage to draw the liquid to be tested. When the pull plate 11 is pushed to push the piston 7, the plunger 26 will move towards the liquid inlet 25 under the action of the spring 30, so that the liquid inlet channel 27 and liquid outlet channel 31 will not contact the liquid guide channel 28, forming an open circuit. At this time, the liquid to be tested will enter the inlet pipe 19 from the leak port 6 and finally flow into the chip substrate 21 at the lower end.

[0041] Furthermore, the sampling mechanism includes a piston rod 14 fixedly connected to one end of the pull rod 5. An annular groove 15 is formed in the side wall of one end of the piston rod 14. A groove is formed in the glass slide cover plate 1. One end of the piston rod 14 slides in the groove. A pull plate 11 is provided on one side of the glass slide cover plate 1. A slot is formed in the interior of one side of the pull plate 11. The slot is fitted onto the outside of the other end of the piston rod 14. A fixing mechanism is provided in the pull plate 11.

[0042] Furthermore, the upper and lower ends of the inlet pipe 19 are respectively connected to the upper wall of the liquid inlet chamber 4 and the medicine tank 20, and the upper ends of several outlet pipes 23 are connected to the lower wall of the medicine tank 20. The inlet pipe 19 is provided with a one-way permeation membrane mechanism.

[0043] Furthermore, the fixing mechanism includes a first sliding groove formed in the pull plate 11, in which a sliding rod 13 is slidably inserted. One end of the sliding rod 13 extends into the outside of the pull plate 11 and the other end is fixedly connected to a second spring 12. One end of the second spring 12 is connected to the inner wall of the first sliding groove. A second sliding groove is formed in the side wall of the first sliding groove, and a third sliding groove is formed in one side wall of the second sliding groove. The third sliding groove is connected to the slot. A connecting rod 17 is slidably arranged in the second sliding groove. One end of the connecting rod 17 is connected to the sliding rod 13, and the other end is connected to a plug 16 on its side wall. The plug 16 slides in the third sliding groove and one end is inserted into the annular sliding groove 15.

[0044] Furthermore, the one-way permeation membrane mechanism includes an elastic resin membrane 33 fixedly installed inside the inlet tube 19. The elastic resin membrane 33 has a plurality of through holes 34. Each through hole 34 includes an upper opening 35 and a lower opening 36. A connection port 37 is connected between the upper opening 35 and the lower opening 36. The diameter of the lower opening 36 is smaller than the diameter of the upper opening 35 and larger than the diameter of the connection port 37.

[0045] When the elastic resin diaphragm 33 bulges upward, the lower opening 36 is squeezed and closed. When the elastic resin diaphragm 33 is concave downward, the lower opening 36 opens, while the upper opening 35 does not close, thus creating a one-way conduction effect.

[0046] Furthermore, the pull plate 11 has inserts 9 at both ends, and the glass slide cover plate 1 has a pair of slots 8 inside. The pair of inserts 9 are inserted into the pair of slots 8. The inner walls on both sides of the inserts 9 are provided with arc-shaped grooves. The inner walls on both sides of the slots 8 are provided with grooves. The pair of grooves are provided with ball bearings 10. The pair of ball bearings 10 are inserted into the pair of arc-shaped grooves. The pair of ball bearings 10 are connected to a third spring. The pair of third springs are connected to the inner walls of the grooves.

[0047] A detection method for a bio-detection chip, the detection method comprising:

[0048] S1: First, attach the nylon membrane 24 containing the biochemical reaction organism to the chip substrate 21. Then, insert the chip substrate 21 into the glass slide base plate 18. When attaching the nylon membrane 24, ensure the spacing between them so that after insertion, the outlet of each tube 23 will correspond to the nylon membrane 24 respectively.

[0049] S2: Then insert the syringe 3 into the solution to be tested, and then pull the pull plate 11 outward. The pull plate 11 can drive the piston column 14 to move outward. The piston column 14 drives the piston 7 to move through the pull rod 5. Under pressure, the solution to be tested will enter the inlet tank 25 from the syringe 3 and enter the inlet chamber 4 along the guide tank 28 and the outlet tank 32.

[0050] S3: Then push the pull plate 11, and the solution to be tested will enter the medicine tank 20 through the leak port 6 and the inlet pipe 19, and finally flow out from the outlet pipe 23 to contact the nylon membrane 24 below, completing the testing process.

[0051] Furthermore, when the piston 7 moves inward along the inlet chamber 4, the elastic resin diaphragm 33 bulges upward due to the air pressure from below the inlet pipe 19. At this time, the lower opening 36 at the lower end of the through hole 34 closes under the elastic action of the elastic resin diaphragm 33, so that the entire through hole 34 is in a closed state. When the piston 7 moves outward along the inlet chamber 4, the elastic resin diaphragm 33 is subjected to the downward pressure of the test solution from above the inlet pipe 19. At this time, the elastic resin diaphragm 33 is concave downward, and the lower opening 36 opens. Since the opening diameter of the upper opening 35 is larger than that of the lower opening 36, the upper opening 35 does not close when the elastic resin diaphragm 33 is concave, so that the elastic resin diaphragm 33 has a one-way conduction effect.

[0052] Those skilled in the art can connect the components in this case sequentially. The specific connection and operation sequence should refer to the working principle described below. The detailed connection methods are well-known technologies in the field. The working principle and process are mainly described below.

[0053] Example: First, a nylon membrane 24 containing biochemical reaction organisms is attached to a chip substrate 21. Then, the chip substrate 21 is inserted into a glass slide base plate 18. When attaching the nylon membrane 24, the spacing between them must be ensured so that after insertion, the outlet of each tube 23 will correspond to the nylon membrane 24 respectively.

[0054] Then, the needle 3 is inserted into the solution to be tested, and then the pull plate 11 is pulled outward. The pull plate 11 can drive the piston column 14 to move outward. The piston column 14 drives the piston 7 to move through the pull rod 5. Under pressure, the solution to be tested will enter the inlet tank 25 from the needle 3 and enter the inlet chamber 4 along the guide tank 28 and the outlet tank 32.

[0055] Then push the pull plate 11, and the solution to be tested will enter the drug tank 20 through the leak port 6 and the inlet pipe 19, and finally flow out from the outlet pipe 23 to contact the nylon membrane 24 below, completing the testing process.

[0056] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A bio-detection chip, characterized in that, The chip includes: a chip substrate (21), a glass slide base plate (18) is fitted on the outside of the chip substrate (21), a glass slide cover plate (1) is installed on the glass slide base plate (18), a plurality of nylon films (24) are arranged on the chip substrate (21), and molecular microarrays are arranged on the plurality of nylon films (24), and a fixing plate (22) is connected to one end of the chip substrate (21), and the fixing plate (22) is fixedly inserted into the glass slide base plate (18); The glass slide cover plate (1) is provided with a plurality of liquid inlet chambers (4), and liquid inlet heads (2) are respectively installed on the liquid inlet ends of the plurality of liquid inlet chambers (4). A needle tube (3) is respectively installed at the front end of the plurality of liquid inlet heads (2). A one-way check mechanism is provided in the plurality of liquid inlet heads (2). A piston (7) is movably installed in the plurality of liquid inlet chambers (4). A pull rod (5) is fixedly connected to one end of the plurality of pistons (7). A sampling mechanism is respectively installed at one end of the plurality of pull rods (5). The slide base plate (18) is provided with a plurality of medicine tanks (20), and each of the medicine tanks (20) is provided with a liquid guiding mechanism; The liquid guiding mechanism includes an inlet tube (19) and several outlet tubes (23) opened in the glass slide base plate (18). The upper end of the inlet tube (19) is provided with a leakage port (6) at the connection between it and the liquid inlet chamber (4). The lower end of the outlet tube (23) is located above the nylon membrane (24).

2. The biodetection chip according to claim 1, characterized in that, The one-way check mechanism includes an inlet groove (25) and an outlet groove (32) inside the inlet head (2). A guide groove (28) is connected between the inlet groove (25) and the outlet groove (32). The inlet head (2) has a cavity. A plunger (26) is movably installed in the cavity. The two ends of the plunger (26) are respectively movably inserted into the inlet groove (25) and the outlet groove (32) and respectively have an inlet channel (27) and an outlet channel (31). A limiting plate (29) is fixedly fitted on the outside of the plunger (26). A spring (30) is fitted on the outside of the plunger (26). The two ends of the spring (30) are in contact with the limiting plate (29) and the inner wall of the cavity, respectively.

3. The biodetection chip according to claim 1, characterized in that, The sampling mechanism includes a piston rod (14) fixedly connected to one end of the pull rod (5). An annular groove (15) is provided in the side wall of one end of the piston rod (14). A groove is provided in the glass slide cover plate (1). One end of the piston rod (14) slides in the groove. A pull plate (11) is provided on one side of the glass slide cover plate (1). A slot is provided in the inside of one side of the pull plate (11). The slot is fitted onto the outside of the other end of the piston rod (14). A fixing mechanism is provided in the pull plate (11).

4. The biodetection chip according to claim 1, characterized in that, The upper and lower ends of the inlet pipe (19) are connected to the upper wall of the liquid inlet chamber (4) and the medicine tank (20) respectively, and the upper ends of several outlet pipes (23) are connected to the lower wall of the medicine tank (20). The inlet pipe (19) is provided with a one-way permeation membrane mechanism.

5. The biodetection chip according to claim 3, characterized in that, The fixing mechanism includes a first slide groove opened in the pull plate (11), a slide rod (13) is slidably inserted in the first slide groove, one end of the slide rod (13) extends into the outside of the pull plate (11) and the other end is fixedly connected to a second spring (12), one end of the second spring (12) is connected to the inner wall of the first slide groove, a second slide groove is opened in the side wall of the first slide groove, a third slide groove is opened in one side wall of the second slide groove, the third slide groove is connected to the slot, a connecting rod (17) is slidably arranged in the second slide groove, one end of the connecting rod (17) is connected to the slide rod (13) and the other end is connected to a plug (16) on the side wall, the plug (16) slides in the third slide groove and one end is inserted into the annular slide groove (15).

6. The biodetection chip according to claim 4, characterized in that, The one-way permeation membrane mechanism includes an elastic resin membrane (33) fixedly installed in the inlet tube (19). The elastic resin membrane (33) has a plurality of through holes (34). The through holes (34) include an upper opening (35) and a lower opening (36). A connecting port (37) is connected between the upper opening (35) and the lower opening (36). The diameter of the lower opening (36) is smaller than the diameter of the upper opening (35) and larger than the diameter of the connecting port (37).

7. The biodetection chip according to claim 3, characterized in that, The pull plate (11) has inserts (9) at both ends. The glass cover plate (1) has a pair of sockets (8) inside. The pair of inserts (9) are inserted into the pair of sockets (8). The inner walls of the two sides of the inserts (9) are provided with arc grooves. The inner walls of the two sides of the sockets (8) are provided with grooves. The pair of grooves are provided with ball bearings (10). The pair of ball bearings (10) are inserted into the pair of arc grooves. The pair of ball bearings (10) are connected to a third spring. The pair of third springs are connected to the inner walls of the grooves.

8. A detection method for a bio-detection chip according to any one of claims 1-7, characterized in that, The detection method includes: S1: First, attach the nylon membrane (24) containing the biochemical reaction organism to the chip substrate (21), and then insert the chip substrate (21) into the glass slide base plate (18). When attaching the nylon membrane (24), ensure the spacing between them so that after insertion, the outlet of each tube (23) will correspond to the nylon membrane (24). S2: Then insert the syringe (3) into the solution to be tested, and then pull the pull plate (11) outward. The pull plate (11) can drive the piston column (14) to move outward. The piston column (14) drives the piston (7) to move through the pull rod (5). Under pressure, the solution to be tested will enter the inlet tank (25) from the syringe (3) and enter the inlet chamber (4) through the outlet tank (32) along the guide tank (28). S3: Then push the pull plate (11), and the solution to be tested will enter the medicine tank (20) through the inlet pipe (19) from the leak (6), and finally flow out from the outlet pipe (23) to contact the nylon membrane (24) below, thus completing the testing process.

9. The detection method of the bio-detection chip according to claim 8, characterized in that, When the piston (7) moves inward along the liquid inlet chamber (4), the elastic resin diaphragm (33) will bulge upward due to the air pressure from below the inlet pipe (19). At this time, the lower opening (36) at the lower end of the through hole (34) will close under the elastic action of the elastic resin diaphragm (33), so that the entire through hole (34) is in a closed state. When the piston (7) moves outward along the liquid inlet chamber (4), the elastic resin diaphragm (33) will be subjected to the downward pressure of the test solution from above the inlet pipe (19). At this time, the elastic resin diaphragm (33) will be concave downward, and the lower opening (36) will open. Since the opening diameter of the upper opening (35) is larger than that of the lower opening (36), the upper opening (35) will not close when the elastic resin diaphragm (33) is concave, so that the elastic resin diaphragm (33) has a one-way conduction effect.

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