Composition for promoting lipid regulation, application and skin care product

Abstract

The present invention belongs to the technical field of skin care, and discloses a lipid regulation promoting composition, application and skin care product, wherein the lipid regulation promoting composition comprises 0.1-2.4 parts of natural vegetable oil and 0.1-2.4 parts of potassium salt by weight. The present invention adopts natural vegetable oil and potassium salt to compound and synergistically activate Staphylococcus epidermidis on the surface of the skin, thereby promoting the related lipase to strengthen and promote the expression of saturated fatty acids, promoting the skin to regulate the lipid component structure, and effectively avoiding the damage of the skin barrier.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to a lipid regulation promoting composition, application and skin care product. Background Art

[0002] According to some literature reports, due to the influence of hormones during pregnancy, the lipids on the skin surface will change. Compared with the lipid component ratio of normal female facial skin, the proportion of saturated fatty acids in the facial lipid components of pregnant women will decrease, thus causing an imbalance in the proportion of facial lipid components of pregnant women, thereby causing damage to the skin barrier.

[0003] Based on this, prior art 1: Chinese patent application 201980068045.3 discloses a skin barrier strengthening composition, which can strengthen the skin barrier by reducing skin moisture loss and improving the skin barrier damaged by ultraviolet radiation; the above patent application strengthens the skin barrier by controlling skin moisture loss, which is a good skin care idea.

[0004] Similarly, prior art 2: Chinese patent application 201811444280.X discloses a skin barrier repair composition and a skin barrier repair product, which comprises: 1-30 parts by weight of a first active ingredient, 0-20 parts by weight of a second active ingredient, 0.5-3 parts by weight of a Panax notoginseng flower extract, 0.1-3 parts by weight of a lavender extract, and 0.01-2 parts by weight of a Paris polyphylla extract, the first active ingredient being selected from at least one of sacha inchi oil and sacha inchi oil microcapsule powder, the second active ingredient being selected from at least one of phytosterols and phytosterol microcapsule powder, and having safe and effective skin barrier repairing properties through reasonable component selection and reasonable component ratio, which can effectively solve the problem of repairing the skin barrier after damage, and can also improve skin resistance; the technical solution of the above patent application repairs the skin barrier and improves damaged skin through a combination of multiple components, and adopts a different approach from prior art 1 to enhance the skin barrier function.

[0005] However, both prior art 1 and prior art 2 are remedial solutions when the skin barrier function is damaged, and no targeted skin care is provided for the physical changes of pregnant women themselves. Therefore, in order to avoid the problem of imbalance in the proportion of facial lipid components in pregnant women, it is necessary to develop a skin care composition suitable for pregnant women from the perspective of skin lipid regulation.

[0006] Staphylococcus epidermidis is a naturally occurring colonizer in the human skin microbiome that can stimulate the skin's immune response. Many studies have suggested that Staphylococcus epidermidis is a benign or beneficial member of the skin microbiome that participates in barrier development, maintains skin balance, maintains homeostasis, controls opportunistic pathogens, and prevents infection by opportunistic pathogens.

[0007] Staphylococcus epidermidis regulates the secretion of lipase, thereby prompting lipase to produce more saturated fatty acids. Skin keratinocytes can sense the fatty acids released by Staphylococcus epidermidis (S. epidermidis), regulate their own growth rate and inflammatory response, and help regulate and maintain normal skin barrier function.

[0008] Therefore, from the perspective of activating Staphylococcus epidermidis, we can help the skin regulate its lipid component structure, thereby solving the problem of imbalance in the proportion of facial lipid components in pregnant women. Summary of the invention

[0009] One of the purposes of the present invention is to provide a lipid regulation promoting composition to solve the problem that the prior art has not been developed in the direction of imbalance in lipid component ratios to avoid damage to the skin barrier. The lipid regulation promoting composition of the present invention helps the skin regulate the lipid component structure from the perspective of activating Staphylococcus epidermidis.

[0010] Another object of the present invention is to provide an application of a lipid regulation promoting composition, wherein the lipid regulation promoting composition is used as a skin lipid component structure regulating component in external application medicines and skin care products, thereby achieving the effect of promoting skin lipid regulation and effectively avoiding damage to the skin barrier.

[0011] At the same time, the present invention also provides a skin care product, which is particularly suitable for skin care of pregnant women and can effectively solve the problem of imbalance in the proportion of facial lipid components of pregnant women.

[0012] To achieve the above object, the present invention provides a lipid regulation promoting composition, which comprises 0.1-2.4 parts by weight of natural vegetable oil and 0.1-2.4 parts by weight of potassium salt, and the total weight of natural vegetable oil and potassium salt is ≤2.5 parts.

[0013] Preferably, the natural vegetable oil includes one or more of coconut oil, palm oil, meadowfoam seed oil, jojoba seed oil, and sweet almond oil.

[0014] Preferably, the potassium salt includes one or more of potassium chloride, dipotassium glycyrrhizinate, potassium aspartate, potassium PCA, and dipotassium EDTA.

[0015] Further preferably, the lipid regulation promoting composition comprises 0.1-2.4 parts of coconut oil and 0.1-2.4 parts of potassium chloride.

[0016] Preferably, the weight proportions of the coconut oil may be selected as: 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1.0 part, 1.1 part, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2.0 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts.

[0017] Preferably, the weight proportions of the potassium chloride can be selected as: 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1.0 part, 1.1 part, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2.0 parts, 2.1 parts, 2.2 parts, 2.3 parts, 2.4 parts, 2.5 parts.

[0018] Coconut oil is a commonly used emollient and is often used in cosmetics to moisturize the skin. As a natural plant oil, it can help the skin form a sebum film on the surface, enhance the skin's resistance and protect moisture from loss. Coconut oil also has certain antioxidant functions, which can help skin cells arrange tightly and orderly, enhancing skin vitality.

[0019] Potassium chloride is used in cosmetics. On the one hand, it can regulate the ion concentration of the cosmetics themselves, which helps to stabilize and absorb some active ingredients in the cosmetics. It can also help the cosmetics form a stable particle structure, which can effectively extend the service life and absorption effect of the cosmetics. On the other hand, potassium chloride of appropriate concentration can also help regulate the ion balance on the skin surface, which helps the skin to better dynamically maintain the epidermal microenvironment.

[0020] The present invention also provides an application of a lipid regulation promoting composition, wherein the lipid regulation promoting composition is used as a skin lipid component structure regulating component in external application medicines and skin care products.

[0021] At the same time, the present invention also provides a skin care product, which contains the above-mentioned lipid regulation promoting composition, and the content of the lipid regulation promoting composition is 2-2.5%.

[0022] Further preferably, the content of the lipid regulation promoting composition can be 2%, 2.1%, 2.2%, 2.3%, 2.4%, or 2.5%.

[0023] Beneficial Effects

[0024] Compared with the prior art, the present invention has at least the following advantages:

[0025] (1) The present invention uses a combination of natural plant oil and potassium salt to synergistically activate Staphylococcus epidermidis on the skin surface, thereby promoting the related lipase to enhance and promote the expression of saturated fatty acids, promoting the skin to regulate the lipid component structure, and effectively avoiding damage to the skin barrier;

[0026] (2) The present invention finds that when coconut oil and potassium chloride are used in combination, their synergistic activation of Staphylococcus epidermidis on the skin surface can be maximized, which is significantly improved compared to the effect of other natural plant oils and potassium salts in promoting the regulation of lipid component structure in the skin;

[0027] (3) The present invention finds that palm oil and potassium chloride can only exert a very significant synergistic effect of promoting the regulation of skin lipid component structure when compounded in a specific ratio, while the effect of activating Staphylococcus epidermidis on the skin surface produced by other ratios is relatively low.

[0028] (4) The lipid regulation promoting composition of the present invention can be applied to various skin care, therapeutic drugs, skin care products and other external application products, and can be used for a long time to maintain the skin barrier function;

[0029] (5) When the lipid regulation promoting composition of the present invention is used in skin care products, the content of the lipid regulation promoting composition in the skin care products is 2-2.5%, thereby maximizing the effect of promoting the regulation of the lipid component structure of the skin. DETAILED DESCRIPTION

[0030] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0031] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0032] In the following examples and comparative examples, potassium chloride, sodium chloride and calcium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd., coconut oil was purchased from Aoyuan New Materials (Guangzhou) Co., Ltd., palm oil was purchased from Guangzhou Aoxue Chemical Co., Ltd., meadowfoam seed oil was purchased from Guangzhou Jucheng Chemical Co., Ltd., dipotassium glycyrrhizate was purchased from Qinghai Lake Pharmaceutical Co., Ltd., Qinghai Province, potassium aspartate was purchased from Shanghai McLean Biochemical Technology Co., Ltd., caprylic / capric triglyceride was purchased from Guangzhou Wanlu Chemical Co., Ltd., and polydimethylsiloxane was purchased from Guangzhou Hengtao Trading Co., Ltd.

[0033] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0034] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0035] Example 1

[0036] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 0.1% coconut oil and 2.4% potassium chloride.

[0037] Example 2

[0038] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 0.5% coconut oil and 1.5% potassium chloride.

[0039] Example 3

[0040] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 1.0% coconut oil and 1.0% potassium chloride.

[0041] Example 4

[0042] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 1.5% coconut oil and 1.0% potassium chloride.

[0043] Example 5

[0044] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 1.0% coconut oil and 1.5% potassium chloride.

[0045] Example 6

[0046] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 1.5% coconut oil and 0.5% potassium chloride.

[0047] Example 7

[0048] The invention discloses a culture medium containing a composition for promoting lipid regulation. The oligonutrient culture medium is prepared to contain a mixture of 2.4% coconut oil and 0.1% potassium chloride.

[0049] Example 8

[0050] The invention discloses a culture medium, which is prepared by using oligonutrient culture medium and contains a mixture of 0.1% coconut oil and 2.4% dipotassium glycyrrhizinate.

[0051] Example 9

[0052] The invention discloses a culture medium, which is prepared by using oligonutrient culture medium and contains a mixture of 0.5% coconut oil and 1.5% dipotassium glycyrrhizinate.

[0053] Example 10

[0054] The invention discloses a culture medium, which adopts oligonutrient culture medium to prepare a mixture containing 1.5% coconut oil and 0.5% dipotassium glycyrrhizinate.

[0055] Embodiment 11

[0056] The invention discloses a culture medium, which adopts oligonutrient culture medium to prepare a mixture containing 2.4% coconut oil and 0.1% dipotassium glycyrrhizinate.

[0057] Example 12

[0058] The invention discloses a culture medium, which adopts an oligonutrient culture medium to prepare a mixture containing 0.1% coconut oil and 2.4% potassium aspartate.

[0059] Embodiment 13

[0060] The invention discloses a culture medium, which adopts an oligonutrient culture medium to prepare a mixture containing 0.5% coconut oil and 1.5% potassium aspartate.

[0061] Embodiment 14

[0062] The invention discloses a culture medium, which adopts an oligonutrient culture medium to prepare a mixture containing 1.5% coconut oil and 0.5% potassium aspartate.

[0063] Embodiment 15

[0064] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 2.4% coconut oil and 0.1% potassium aspartate.

[0065] Example 16

[0066] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 0.1% palm oil and 2.4% potassium chloride.

[0067] Embodiment 17

[0068] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 0.5% palm oil and 1.5% potassium chloride.

[0069] Embodiment 18

[0070] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 1.5% palm oil and 1.0% potassium chloride.

[0071] Embodiment 19

[0072] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 2.4% palm oil and 0.1% potassium chloride.

[0073] Embodiment 20

[0074] The invention discloses a culture solution, which is prepared by using oligonutrient culture solution and contains a mixture of 0.1% meadowfoam seed oil and 2.4% potassium chloride.

[0075] Embodiment 21

[0076] The invention discloses a culture solution, which is prepared from an oligonutrient culture solution containing a mixture of 0.5% meadowfoam seed oil and 1.5% potassium chloride.

[0077] Embodiment 22

[0078] The invention discloses a culture solution, which is prepared from an oligonutrient culture solution containing a mixture of 1.5% meadowfoam seed oil and 1.0% potassium chloride.

[0079] Embodiment 23

[0080] The invention discloses a culture solution, which is prepared by using an oligonutrient culture solution and contains a mixture of 2.4% of meadowfoam seed oil and 0.1% of potassium chloride.

[0081] Comparative Example 1

[0082] A culture medium is prepared by using an oligonutrient culture medium and containing a 2.5% coconut oil mixture.

[0083] Comparative Example 2

[0084] A culture solution is prepared by using an oligonutrient culture solution and containing a mixture of 2.5% potassium chloride.

[0085] Comparative Example 3

[0086] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 3.0% coconut oil and 0.1% potassium chloride.

[0087] Comparative Example 4

[0088] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 0.1% coconut oil and 3.0% potassium chloride.

[0089] Comparative Example 5

[0090] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 1.5% coconut oil and 0.5% sodium chloride.

[0091] Comparative Example 6

[0092] A culture medium is prepared by using an oligonutrient culture medium containing a mixture of 1.5% coconut oil and 0.5% calcium chloride.

[0093] Comparative Example 7

[0094] The invention discloses a culture medium, which adopts an oligonutrient culture medium to prepare a mixture containing 1.5% caprylic / capric triglyceride and 0.5% potassium chloride.

[0095] Comparative Example 8

[0096] A culture medium is prepared by using oligonutrient culture medium and containing a mixture of 1.5% dimethylsiloxane and 0.5% potassium chloride.

[0097] Performance Testing

[0098] Experimental methods

[0099] Experimental reagents

[0100] 1) Fermentation:

[0101] Oligotrophic medium:

[0102] NaCl 8g / L, KCI 0.2g / L, Na2HPO4 0.24g / L, KH2PO4 0.24g / L, L-Cys 0.5g / L

[0103] Tryptone Soy Broth (TSB)—Qingdao Haibo HB4114

[0104] Tryptone Soy Agar (TSA)—Qingdao Haibo HB0177

[0105] 2) Medium and long chain fatty acid detection:

[0106] Pure methanol, hexane, isopropanol, sulfuric acid—Thermo-Fisher Scientific laboratory supplies

[0107] 1) Fermentation: 50ml centrifuge tube, 1.5ml centrifuge tube, disposable culture dish—NEST 5ml pipette tip, 1ml pipette tip with filter—Axygen

[0108] Blue-mouth bottle, disposable inoculation loop, disposable coating rod, 0.22μm disposable filter 2) Medium and long chain fatty acid detection:

[0109] 1.5ml centrifuge tube, 2ml glass bottle, injection bottle

[0110] GC-MS related consumables:

[0111]

[0112] Experimental instruments

[0113] 1) Fermentation:

[0114] Ultrapure water purifier - Sartorius

[0115] Electronic Analytical Balance—Mettler-Toledo

[0116] Constant temperature shaking incubator - Shanghai Yiheng

[0117] Refrigerated centrifuge—Thermo fisher

[0118] 2) Medium and long chain fatty acid detection:

[0119] MSC-100 Constant Temperature Mixer - Hangzhou Aosheng Instrument Co., Ltd.

[0120] Microfuge 20R Centrifuge—Beckman

[0121] Centrivap Complete Vacuum Concentrator - Labconco

[0122] XSR105DU / A Electronic Analytical Balance—Mettler-Toledo

[0123] Mill-QⅡ Ultrapure Water Apparatus—Milipore

[0124] Pegasus BT Gas Chromatography-Time of Flight Mass Spectrometry (GC-TOFMS)—Leco Corp.

[0125] Experimental Procedure

[0126] Recovery and enrichment culture of Staphylococcus epidermidis

[0127] 1) Pick the frozen S. epidermidis ATCC 12228 strain and streak three zones on tryptic soy broth agar (TSA) plates, and culture them in a 37°C constant temperature incubator overnight (12-24 hours).

[0128] 2) Pick a single colony, inoculate it into 30 ml of TSB liquid culture medium, and culture it in a 37°C incubator overnight (16 h). Inoculate multiple tubes according to the experimental situation.

[0129] 3) Centrifuge at 4000 rpm for 10 minutes (20°C), discard the supernatant. Obtain bacterial microspheres, resuspend each tube with 5 ml of the corresponding culture medium (oligotrophic, nutrient-rich). If there are multiple tubes, combine them into a 50 ml centrifuge tube, oscillate for 10 seconds to mix evenly and set aside.

[0130] Co-culture system of crude extract of Staphylococcus epidermidis enzyme-oil-test raw materials

[0131] 1) Preparation of the test system: Prepare the test raw materials of corresponding concentrations using oligonutrient culture medium, and add the prepared oil of corresponding concentrations, mix well, sterilize at 115°C and high temperature and high pressure for 20 minutes, and cool at room temperature for use;

[0132] 2) Centrifuge the overnight culture of Staphylococcus epidermidis at 7000g for 10 min at 4°C to obtain a crude enzyme extract [1].

[0133] 3) Add 9 ml of the test system to each tube, and add 1 ml of the crude extract of Staphylococcus epidermidis enzyme to each tube of fermentation system. After mixing, incubate in a 37°C constant temperature shaker (220 rpm) for 24 hours. The system without crude extract, raw materials to be tested and oil, the system with crude extract, oil and no raw materials to be tested, and the system with crude extract but no raw materials to be tested and oil were used as controls.

[0134] 4) After fermentation is completed, the fermentation broth is filtered through a 0.22 μm filter to collect the fermentation supernatant for the detection of medium- and long-chain fatty acids.

[0135] Medium and long chain fatty acid detection

[0136] 1) Accurately measure the standard and dissolve it in methanol to prepare a mother solution with a concentration of 1 mg / ml. When using, mix the mother solution and dilute it into a series of concentrations of standard samples to obtain a standard curve.

[0137] 2) To reduce degradation, the sample was thawed in an ice bath. Take 200 μL of supernatant sample to a 1.5 ml centrifuge tube, add 500 μL of isopropanol, and shake at 1450 rpm for 15 min at 10 ° C. Centrifuge at 18000g for 25 min at 4 ° C. Transfer 400 μL of supernatant to a 2 ml glass bottle and vacuum concentrate to dryness. Add 200 μL of 2% sulfuric acid methanol solution, and place the sample at 80 ° C and shake at 650 rpm for 30 min. After the reaction is completed, cool the sample to room temperature, add 200 μL of n-hexane, sonicate for 1 min, and then shake at 1400 rpm for 10 min. Let the sample stand at 4 ° C for 20 min, take the upper layer of n-hexane and transfer it to the injection bottle and wait for injection analysis.

[0138] 3) The raw data generated by GC-MS will be baseline corrected, smoothed, peak found, deconvoluted, integrated, calibrated, and quantitatively analyzed for each metabolite using ChromaTOF software (v5.51, Leco corp., USA).

[0139] 4) Calculate the linear relationship between the instrument response and the concentration to obtain y = ax + b (y represents the instrument response, such as peak height, peak area. a represents the slope and / or sensitivity, and b represents a constant describing the background); the concentration of the unknown metabolite (x) will be calculated using this formula.

[0140] The compositions prepared in Examples 1-7 and Comparative Examples 1-24 were tested according to the above-mentioned experimental method, and the results are shown in Table 1;

[0141] Table 1 Test results of embodiments and comparative examples

[0142]

[0143]

[0144] According to the results in Table 1, we can see that:

[0145] It should be noted that, from the perspective of imbalance in skin lipid structure, there is a lack of saturated fatty acids during pregnancy, especially saturated fatty acids with relatively shorter carbon chains. Therefore, the technical focus of the present invention is mainly on the changes in the contents of capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0).

[0146] According to the data of Examples 1-23, the present invention can help activate Staphylococcus epidermidis on the skin surface by using a combination of natural plant oil and potassium salt in any proportion within the concentration range of 2.0-2.5% in skin care products, thereby promoting the enhancement of related lipases and the expression of saturated fatty acids.

[0147] Examples 1-7 show that the combination of coconut oil and potassium chloride can synergistically promote the enhancement of related lipases and promote the expression of saturated fatty acids, including capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0) The content is significantly increased, which is helpful to help make up for the lack of saturated fatty acids in the skin during pregnancy, especially the problem of medium and short-chain fatty acid deficiency; palmitic acid (hexadecanoic acid, C16:0) and stearic acid (octadecanoic acid, C18:0) are also synergistically promoted, but the effect is not as obvious as the first three fatty acids; arachidic acid (eicosanoic acid, C20:0) has almost no effect; and oleic acid (octadecenoic acid, C18:1 (cis-9)) is very significantly inhibited, which is helpful to reduce the damage to the skin barrier due to excessive oleic acid content, and inhibit steady-state induced erythema by destroying the lipid structure of the stratum corneum.

[0148] Examples 8-15 show that coconut oil and other potassium salts such as dipotassium glycyrrhizinate and potassium aspartate also have the effect of synergistically promoting the enhancement of skin-related lipase and promoting the expression of saturated fatty acids, but compared with the combination of coconut oil and potassium chloride, the synergistic effect is relatively weak. According to the data of the blank control, the synergistic enhancement effect is also significant, and the increase in the content of capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0) is also more obvious; and when Examples 10, 11, 14 and 15 use a lower proportion of dipotassium glycyrrhizinate and potassium aspartate and a higher proportion of coconut oil, the synergistic promotion of expression of palmitic acid (hexadecanoic acid, C16:0) and stearic acid (octadecanoic acid, C18:0) is significantly increased relative to the composite combination of coconut oil and potassium chloride.

[0149] But in general, the combination of coconut oil and potassium chloride has a more significant effect in synergistically promoting the enhancement of related lipases and promoting the expression of saturated fatty acids. It can maximize the efficacy of the combination of natural plant oils and potassium salts and supplement the saturated fatty acids lacking during pregnancy.

[0150] According to the comparison between Examples 16-19 and the blank control, the combination of palm oil and potassium chloride can also effectively synergistically promote the enhancement of skin-related lipase and the expression of saturated fatty acids, but its effect of increasing the content of capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0) is lower than that of the combination of coconut oil and other potassium salts. On the contrary, its synergistic promotion effect on the expression of palmitic acid (hexadecanoic acid, C16:0) and stearic acid (octadecanoic acid, C18:0) is very obvious.

[0151] According to the data comparison between Examples 16-19 and Comparative Example 2, Examples 16-19 expressed a certain synergistic effect in increasing the content of capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0), but the synergistic effect was not significant; while Examples 16-19 showed a significant improvement in the synergistic promotion of palmitic acid (hexadecanoic acid, C16:0) and stearic acid (octadecanoic acid, C18:0), and also showed a significant inhibitory effect of oleic acid (octadecenoic acid, C18:1 (cis-9)) compared to Comparative Example 2.

[0152] According to Examples 20-23, the combination of Limnanthes bidentata seed oil and potassium chloride also has a significant synergistic effect in promoting the skin to regulate the lipid component structure, but its effect is lower than that of the combination of coconut oil and potassium chloride, indicating that the combination of coconut oil and potassium chloride can maximize the synergistic effect of promoting the skin to regulate the lipid component structure.

[0153] According to the data comparison between Examples 1-7 and Comparative Examples 1 and 2, it can be seen that the present invention uses coconut oil and potassium chloride to produce a synergistic effect of promoting the regulation of the lipid component structure of the skin.

[0154] According to the data of Comparative Examples 3 and 4, when the concentration range of the present invention is exceeded, although the compounding of coconut oil and potassium chloride can still effectively synergistically promote the increase in the content of capric acid (decanoic acid, C10:0), lauric acid (dodecanoic acid, C12:0) and myristic acid (tetradecanoic acid, C14:0), and palmitic acid (hexadecanoic acid, C16:0) and stearic acid (octadecanoic acid, C18:0) are also significantly increased, but the inhibitory effect of oleic acid (octadecenoic acid, C18:1 (cis-9)) is lost, and the protective effect of the skin barrier is greatly reduced at this time, so it is not the optimal concentration range of skin care products.

[0155] According to the comparison between Example 6 and Comparative Examples 1, 2, 5 and 6, it can be seen that potassium salt can synergistically promote the skin regulating lipid component structure with natural plant oils, and the use of other metal salts not only fails to synergistically promote the skin regulating lipids, but also significantly reduces the skin lipid regulating effect.

[0156] Similarly, according to the comparison between Example 6 and Comparative Examples 1, 2, 7 and 8, it can be seen that synthetic oils and silicone oils cannot replace the synergistic effect of natural vegetable oils and potassium salts.

[0157] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A composition for preparing a skin care product for activating Staphylococcus epidermidis to promote lipid regulation, characterized in that: The composition consists of 0.1-2.4 parts of coconut oil and 0.1-2.4 parts of potassium chloride by weight, and the total weight of coconut oil and potassium chloride is ≤2.5 parts.

2. A skin care product, characterized in that: Containing the composition according to claim 1, the content of the composition is 2-2.5%.

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