A composition for improving blood rheology, a method for preparing the same, and use thereof

Abstract

The present application relates to a kind of compositions for improving blood rheology, composition contains flaxseed powder 60-80 parts, bamboo leaf flavone extract 5-15 parts, extract of polygonatum 5-10 parts, wolfberry extract 5-10 parts and prebiotic 5-10 parts, wherein, the prebiotic is composed of fructooligosaccharide:isomaltooligosaccharide according to mass ratio 2-3:2-5, the flaxseed powder contains alpha-linolenic acid 20%-40% and cyanogenic glycoside ≤6ppm, the average particle size of flaxseed powder is 100-300nm, wolfberry extract contains total sugar 60-80%, protein 1-10%, uronic acid 1-10%, the molar ratio of mannose: rhamnose: glucose: galactose: arabinose is 1:0.8:5.9:12.3:14.7.The present application synergistically utilizes multiple effective components (alpha-linolenic acid, polysaccharide, etc.) in composition for regulating blood lipid, blood sugar and blood viscosity, antioxidant, improving memory, improving gastrointestinal movement and enhancing immunity, anti-fatigue, delaying aging, etc., has the beneficial effect of significantly improving blood rheology, memory, improving gastrointestinal movement and enhancing immunity, etc.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medicine, in particular to a composition for improving blood rheology, a preparation method thereof and application thereof. BACKGROUND

[0002] The Global Report on Diabetes published by the World Health Organization (WHO) in 2017 shows that the number of people with diabetes worldwide is increasing year by year and is becoming younger. Its various complications lead to high disability and mortality rates and other serious consequences for patients with diabetes. The immunity of patients with diabetes is often weak, and they are prone to various diseases and serious health-compromising complications, including glucose metabolism disorders, high blood sugar and high sugar content in tissue cells, accumulation of pyruvic acid, which is conducive to the growth and reproduction of bacteria; fat metabolism disorders in patients, increased triglycerides, which provide rich nutrition for bacteria; decreased albumin formation, decreased levels of antibodies and complements in the body, and decreased immunity; increased blood viscosity, impaired microcirculation, affecting oxygen exchange between blood and tissue, leading to decreased resistance of the body; metabolic disorders leading to liver damage, decreased conversion of vitamin A function, resulting in impaired integrity of respiratory mucosa epithelium and decreased defense function, making it easy to be infected by bacteria. Patients with diabetes or their relatives often have other autoimmune diseases, such as diffuse goiter with hyperthyroidism, chronic lymphatic thyroiditis, myasthenia gravis, and pernicious anemia. The incidence of malignant tumors in elderly patients with type II diabetes is significantly increased. Insulin resistance in patients can lead to hyperinsulinemia, and insulin has the effect of promoting the growth of malignant tumors. Therefore, diabetes should be prevented and treated early.

[0003] Studies have shown that dyslipidemia and hyperlipidemia can directly cause diseases that seriously harm human health (such as cardiovascular and cerebrovascular diseases, occlusive vascular diseases, atherosclerosis, coronary heart disease, pancreatitis, etc.), and one or several blood rheology indicators are abnormal (such as increased blood viscosity factors, abnormal blood flow, stagnation, thrombosis, etc.) before obvious clinical signs appear. Timely improvement of blood rheology can effectively prevent and treat diseases caused by blood rheology.

[0004] Linseed is rich in α-linolenic acid and nutrients (protein, dietary fiber), etc., and has the effects of lowering blood sugar, lowering blood lipids, anti-thrombosis, protecting liver, enhancing intelligence and brain, lubricating intestines and defecation, detoxification and weight loss, improving immunity, and enhancing skin elasticity, etc., but the content of raw cyanogenic glycosides should be controlled within a safe range. The wolfberry extract can significantly increase the content of superoxide dismutase (SOD) in blood, liver and muscle tissue, and has the effects of scavenging active oxygen, nourishing kidney essence, benefiting essence and blood, tonifying liver blood, lowering blood lipids and blood sugar, delaying aging, and resisting fatigue, etc. The extract of rhizoma polygonati mainly includes polysaccharides, steroidal saponins, triterpenes, alkaloids, lignans, flavonoids, phytosterols and volatile oils, etc., and has the effects of tonifying kidney and essence, nourishing yin and moistening dryness, regulating blood sugar and blood lipids, etc. The extract of bamboo leaf flavonoids has the effects of antioxidant, regulating blood lipids and blood sugar, regulating whole blood viscosity and plasma viscosity, fibrinogen content and red blood cells, etc. Therefore, it is necessary to develop a composition for effectively improving blood rheology, so as to effectively prevent and treat diseases and health problems caused by blood rheology, and improve the health of the public. SUMMARY

[0005] The present application aims to provide a composition for improving blood rheology, which contains 60-80 parts of linseed powder, 5-15 parts of extract of bamboo leaf flavonoids, 5-10 parts of extract of rhizoma polygonati, 5-10 parts of extract of wolfberry and 5-10 parts of prebiotics, wherein the prebiotics is composed of oligofructose and oligoisomaltose in a mass ratio of 2-3:2-5, the linseed powder contains 20%-40% of α-linolenic acid and ≤6ppm of raw cyanogenic glycosides, the average particle size of the linseed powder is 100-300nm, the extract of wolfberry contains 60-80% of total sugar, 1-10% of protein and 1-10% of uronic acid, and the molar ratio of mannose: rhamnose: glucose: galactose: arabinose is 1:0.8:5.9:12.3:14.7.

[0006] In the preferred technical solution of the present application, the preparation of the linseed powder comprises the following steps:

[0007] (1) 35-45 parts of linseed oil, 0.01-0.05 parts of vitamin E, 1-5 parts of monoglyceride and 1-5 parts of sodium stearoyl lactate are placed under the condition of 60-75℃ for stirring until uniform mixing, and then incubation is performed to prepare an oil phase;

[0008] (2) 5-10 parts of linseed gum, 5-10 parts of malt dextrin, 5-10 parts of sodium caseinate, 5-10 parts of sodium octenyl succinate starch, 0.1-0.5 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of sodium tripolyphosphate and 60-80 parts of water are placed under the condition of 60-75℃ for stirring until uniform mixing, and then incubation is performed to prepare an aqueous phase;

[0009] (3) slowly add the prepared oil phase into the prepared water phase, after shearing emulsification under the condition of 1500-2000 rpm for 15-30 min, then place it under the condition of 28-30 KHz, 1000-1200 W for ultrasonic treatment for 15-30 min, after that, spray dry the prepared emulsion, the inlet temperature is 165-180 ℃, the outlet temperature is 70-85 ℃, then obtain, the linseed powder contains 25-35% of alpha-linolenic acid and <4 ppm of raw cyanogenic glycosides, the average particle size of the linseed powder is 150-250 nm.

[0010] In the preferred technical scheme of the present application, the Huangjing extract contains 10-20% of Huangjing polysaccharide, and the molar ratio of glucose: mannose: galacturonic acid is 6:25.4:1.

[0011] In the preferred technical scheme of the present application, the bamboo leaf flavone extract contains 40-60% of bamboo leaf flavone, preferably 45-55%.

[0012] In the preferred technical scheme of the present application, the preparation of the linseed powder comprises the following steps:

[0013] (1) uniformly mix 38-42 parts of linseed oil, 0.02-0.03 parts of vitamin E, 2-3 parts of monoglyceride and 2-3 parts of sodium stearoyl lactylate under the condition of 65-70 ℃, and keep warm to prepare an oil phase;

[0014] (2) uniformly mix 6-8 parts of linseed gum, 6-8 parts of malt dextrin, 6-8 parts of sodium caseinate, 6-8 parts of sodium octenyl succinate starch, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of sodium tripolyphosphate and 68-75 parts of water under the condition of 65-70 ℃, and keep warm to prepare a water phase;

[0015] (3) slowly add the oil phase into the water phase, after shearing emulsification under the condition of 1500-2000 rpm for 15-30 min, then place it under the condition of 28-30 KHz, 1000-1200 W for ultrasonic treatment for 15-30 min, after that, spray dry the prepared emulsion, the inlet temperature is 170-175 ℃, the outlet temperature is 75-80 ℃, then obtain, the linseed powder contains 28-32% of alpha-linolenic acid and <2 ppm of raw cyanogenic glycosides, the average particle size of the linseed powder is 150-200 nm.

[0016] In the preferred technical scheme of the present application, the composition contains 65-75 parts of linseed powder, 8-12 parts of bamboo leaf flavone extract, 6-8 parts of Huangjing extract, 6-8 parts of medlar extract and 5-8 parts of prebiotic, and the prebiotic is composed of fructooligosaccharide: isomaltooligosaccharide in a mass ratio of 2.5-3:2.5-5.

[0017] In a preferred embodiment of the present invention, the composition contains 69 parts of flaxseed powder, 10 parts of bamboo leaf flavonoid extract, 8 parts of polygonatum extract, 8 parts of wolfberry extract, and 5 parts of prebiotics, wherein the prebiotics are composed of fructooligosaccharides and isomaltooligosaccharides in a mass ratio of 1:1.

[0018] In a preferred embodiment of the present invention, the composition contains 75 parts of flaxseed powder, 8 parts of bamboo leaf flavonoid extract, 6 parts of polygonatum extract, 6 parts of wolfberry extract, and 5 parts of prebiotics, wherein the prebiotics are composed of fructooligosaccharides and isomaltooligosaccharides in a mass ratio of 2:3.

[0019] In a preferred embodiment of the present invention, the composition contains 65 parts of flaxseed powder, 12 parts of bamboo leaf flavonoid extract, 7 parts of polygonatum extract, 8 parts of wolfberry extract, and 8 parts of prebiotics, wherein the prebiotics are composed of fructooligosaccharides and isomaltooligosaccharides in a mass ratio of 3:5.

[0020] The present invention aims to provide a method for preparing a composition for improving blood rheology. The composition contains 60-80 parts flaxseed powder, 5-15 parts bamboo leaf flavonoid extract, 5-10 parts Polygonatum sibiricum extract, 5-10 parts wolfberry extract, and 5-10 parts prebiotics. The prebiotics are composed of fructooligosaccharides and isomaltooligosaccharides in a mass ratio of 2-3:2-5. The flaxseed powder contains 20%-40% α-linolenic acid and ≤6 pp of cyanogenic glycosides. The flaxseed powder has a particle size of 100-300 nm. The wolfberry extract contains 60-80% total sugar, 1-10% protein, and 1-10% uronic acid. The molar ratio of mannose:rhamnose:glucose:galactose:arabinose is 1:0.8:5.9:12.3:14.7. The method includes the following steps: weighing the required amount of flaxseed powder, bamboo leaf flavonoid extract, polygonatum extract, wolfberry extract prebiotic, and mixing them evenly to obtain the final product.

[0021] In a preferred embodiment of the present invention, the preparation of the flaxseed powder includes the following steps:

[0022] (1) Place 35-45 parts of flaxseed oil, 0.01-0.05 parts of vitamin E, 1-5 parts of mono-fatty acid glycerides and 1-5 parts of sodium stearoyl lactylate in a container at 60℃-75℃ and stir until uniformly mixed. Keep warm to obtain the oil phase.

[0023] (2) Mix 5-10 parts of flaxseed gum, 5-10 parts of maltodextrin, 5-10 parts of sodium caseinate, 5-10 parts of sodium octenyl succinate starch, 0.1-0.5 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of sodium tripolyphosphate and 60-80 parts of water at 60℃-75℃ until uniformly mixed, keep warm, and obtain an aqueous phase;

[0024] (3) slowly add the prepared oil phase into the prepared water phase, after shearing emulsification under the condition of 1500-2000 rpm for 15-30 min, then place it under the condition of 28-30 KHz, 1000-1200 W for ultrasonic treatment for 15-30 min, after that, spray dry the prepared emulsion, the inlet temperature is 165-180 ℃, the outlet temperature is 70-85 ℃, then obtain, the flaxseed powder contains 25-35% of alpha-linolenic acid and <4 ppm of raw cyanogenic glycosides, the average particle size of the flaxseed powder is 150-250 nm.

[0025] In the preferred technical scheme of the present application, the Huangjing extract contains 10-20% of Huangjing polysaccharide, and the molar ratio of glucose: mannose: galacturonic acid is 6:25.4:1.

[0026] In the preferred technical scheme of the present application, the bamboo leaf flavone extract contains 40-60% of bamboo leaf flavone, preferably 45-55%.

[0027] In the preferred technical scheme of the present application, the preparation of the flaxseed powder comprises the following steps:

[0028] (1) uniformly mix 38-42 parts of flaxseed oil, 0.02-0.03 parts of vitamin E, 2-3 parts of monoglyceride and 2-3 parts of sodium stearoyl lactylate under the condition of 65-70 ℃, and keep warm to prepare an oil phase;

[0029] (2) uniformly mix 6-8 parts of flaxseed gum, 6-8 parts of malt dextrin, 6-8 parts of sodium caseinate, 6-8 parts of sodium octenyl succinate starch, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of sodium tripolyphosphate and 68-75 parts of water under the condition of 65-70 ℃, and keep warm to prepare a water phase;

[0030] (3) slowly add the oil phase into the water phase, after shearing emulsification under the condition of 1500-2000 rpm for 15-30 min, then place it under the condition of 28-30 KHz, 1000-1200 W for ultrasonic treatment for 15-30 min, after that, spray dry the prepared emulsion, the inlet temperature is 170-175 ℃, the outlet temperature is 75-80 ℃, then obtain, the flaxseed powder contains 28-32% of alpha-linolenic acid and <2 ppm of raw cyanogenic glycosides, the average particle size of the flaxseed powder is 150-200 nm.

[0031] In the preferred technical scheme of the present application, the composition contains 65-75 parts of flaxseed powder, 8-12 parts of bamboo leaf flavone extract, 6-8 parts of Huangjing extract, 6-8 parts of medlar extract and 5-8 parts of prebiotic, and the prebiotic is composed of fructooligosaccharide: isomaltooligosaccharide in a mass ratio of 2.5-3:2.5-5.

[0032] In the preferred technical scheme of the present application, the composition contains 69 parts of flaxseed powder, 10 parts of bamboo leaf flavone extract, 8 parts of rhizoma polygonati extract, 8 parts of medlar extract and 5 parts of prebiotic, wherein the prebiotic is composed of fructooligosaccharide and isomaltooligosaccharide in a mass ratio of 1:1.

[0033] In the preferred technical scheme of the present application, the composition contains 75 parts of flaxseed powder, 8 parts of bamboo leaf flavone extract, 6 parts of rhizoma polygonati extract, 6 parts of medlar extract and 5 parts of prebiotic, wherein the prebiotic is composed of fructooligosaccharide and isomaltooligosaccharide in a mass ratio of 2:3.

[0034] In the preferred technical scheme of the present application, the composition contains 65 parts of flaxseed powder, 12 parts of bamboo leaf flavone extract, 7 parts of rhizoma polygonati extract, 8 parts of medlar extract and 8 parts of prebiotic, wherein the prebiotic is composed of fructooligosaccharide and isomaltooligosaccharide in a mass ratio of 3:5.

[0035] Another object of the present application is to provide the use of the pharmaceutical composition described in the present application for preparing a product for improving blood rheology, improving memory and improving gastrointestinal function, wherein the product is selected from any one of food, functional food and special medical food.

[0036] In the preferred technical scheme of the present application, the user can select the appropriate dosage according to the health status, age, gender, personal needs and other factors.

[0037] Unless otherwise specified, the content of omega-3 unsaturated fatty acid is detected by GB / T 22223-2008 Determination of total fat, saturated fatty acids and unsaturated fatty acids in food, and the content of cyanogenic glycoside is detected by NY / T 3607-2020 Detection of cyanogenic glycoside in agricultural products.

[0038] Unless otherwise specified, when the present application involves a percentage between liquids, the percentage is a volume / volume percentage; when the present application involves a percentage between a liquid and a solid, the percentage is a volume / weight percentage; when the present application involves a percentage between a solid and a liquid, the percentage is a weight / volume percentage; and the rest is a weight / weight percentage.

[0039] Compared with the prior art, the present application has the following beneficial technical effects:

[0040] 1. This invention scientifically combines flaxseed powder, bamboo leaf flavonoid extract, polygonatum extract, wolfberry extract, and prebiotics. It synergistically utilizes various effective ingredients in the composition (α-linolenic acid, polysaccharides, etc.) that regulate blood lipids, blood sugar and blood viscosity, have antioxidant properties, improve memory, improve gastrointestinal motility and enhance immunity, fight fatigue, and delay aging. The composition has the advantages of strong antioxidant capacity, good quality uniformity, high solubility, low hygroscopicity, good stability, high bioavailability, good taste, and good long-term preservation. It has significant beneficial effects such as improving blood rheology, memory, improving gastrointestinal motility and enhancing immunity.

[0041] 2. The preparation method of the present invention has the advantages of simple operation, lower cost, green and environmentally friendly, and suitable for industrial-scale production. Attached Figure Description

[0042] Figure 1 The study investigated the effects of the composition of this invention on blood rheology, including (a) blood viscosity; (b) plasma viscosity; (c) platelet aggregation; and (d) hematocrit. * indicates a significant difference compared to the control group (P < 0.05), and ** indicates a significant difference compared to the control group (P < 0.01).

[0043] Figure 2 This study investigates the memory-improving effects of the composition of this invention. * indicates a significant difference compared to the control group (P < 0.05), and ** indicates a significant difference compared to the control group (P < 0.01).

[0044] Figure 3 The study on the effect of the composition of the present invention on improving gastrointestinal motility shows that * indicates a significant difference compared with the blank group (P<0.05), and ★ indicates a significant difference compared with the control group (P<0.05). Detailed Implementation

[0045] The present invention will be further described below with reference to the embodiments.

[0046] In the specific embodiments, the wolfberry extract (containing 60-80% total sugar, 1-10% protein, 1-10% uronic acid, with a molar ratio of mannose:rhamnose:glucose:galactose:arabinose of 1:0.8:5.9:12.3:14.7), the polygonatum extract (containing 10-20% polygonatum polysaccharide, with a molar ratio of glucose:mannose:galacturonic acid of 6:25.4:1), the bamboo leaf flavonoid extract (containing 51.2% bamboo leaf flavonoids), fructooligosaccharides, and isomaltooligosaccharides were all commercially available.

[0047] Example 1 Preparation of the composition of the present invention

[0048] The composition of the composition of the present invention:

[0049] Example 1 (g) Flaxseed powder 69 Bamboo leaf flavone extract 10 Huangji extract 8 Gouqi extract 8 Fructo-oligosaccharide 2.5 Isomalto-oligosaccharide 2.5 Total 100

[0050] The preparation of the composition of the present application comprises the following steps: weighing the required amount of flaxseed powder, wolfberry extract, polygonatum extract, bamboo leaf flavone extract, fructooligosaccharide and isomaltooligosaccharide, and uniformly mixing them to obtain the composition, wherein the preparation of the flaxseed powder comprises the following steps:

[0051] (1) placing 40 parts of flaxseed oil, 0.03 parts of vitamin E, 3 parts of monoglyceride and 2 parts of sodium stearoyl lactylate in a 65℃ environment and stirring until uniformly mixed, and then keeping the temperature, to obtain an oil phase;

[0052] (2) placing 8 parts of flaxseed gum, 7 parts of malt dextrin, 6 parts of sodium caseinate, 7 parts of sodium octenyl succinate starch, 0.2 parts of potassium dihydrogen phosphate, 0.2 parts of sodium tripolyphosphate and 70 parts of water in a 65℃ environment and stirring until uniformly mixed, and then keeping the temperature, to obtain an aqueous phase;

[0053] (3) slowly adding the oil phase into the aqueous phase, emulsifying for 15-30 minutes by using a high-speed shearing machine at 2500 rpm, and then ultrasonically treating for 15-30 minutes under the condition of 28-30 KHz and 1800-1200 W, and then spray drying the obtained emulsion (the inlet air temperature is 180℃ and the outlet air temperature is 85℃), to obtain the flaxseed powder. The flaxseed powder obtained by the method has 29.8% of α-linolenic acid, ≤1 ppm of cyanogenic glycosides, and an average particle size of 143.6 nm.

[0054] Example 2 The preparation of the composition of the present application

[0055] The composition of the present application comprises:

[0056] Example 2 (g) Flaxseed powder 75 Bamboo leaf flavone extract 8 Huangji extract 6 Gouqi extract 6 Fructo-oligosaccharide 2 Isomalto-oligosaccharide 3 Total 100

[0057] The preparation of the composition of the present application comprises the following steps: weighing the required amount of flaxseed powder, wolfberry extract, polygonatum extract, bamboo leaf flavone extract, fructooligosaccharide and isomaltooligosaccharide, and uniformly mixing them to obtain the composition, wherein the preparation of the flaxseed powder comprises the following steps:

[0058] (1) placing 42 parts of flaxseed oil, 0.03 parts of vitamin E, 3 parts of monoglyceride and 2 parts of sodium stearoyl lactylate in a 70℃ environment and stirring until uniformly mixed, and then keeping the temperature, to obtain an oil phase;

[0059] (2) placing 8 parts of flaxseed gum, 7 parts of malt dextrin, 7 parts of sodium caseinate, 7 parts of sodium octenyl succinate starch, 0.2 parts of potassium dihydrogen phosphate, 0.2 parts of sodium tripolyphosphate and 69 parts of water in a 70℃ environment and stirring until uniformly mixed, and then keeping the temperature, to obtain an aqueous phase;

[0060] (3) slowly add the oil phase into the water phase, emulsify with 1800 rpm high speed shearing machine for 15-30 min, then ultrasonic treat under the condition of 28-30 KHz, 1500-1200 W for 15-30 min, spray dry the prepared emulsion (the inlet air temperature is 175 ℃, the outlet air temperature is 80 ℃), and then the product is obtained. According to the method of the present application, the prepared flaxseed powder contains 29.5% of α-linolenic acid, glucosinolates ≤2 ppm, and the average particle size of the flaxseed powder is 152.7 nm.

[0061] Example 3 Preparation of the composition of the present application

[0062] Composition of the composition of the present application:

[0063] Example 3 (g) Flaxseed powder 65 Bamboo leaf flavone extract 12 Huangji extract 7 Gouqi extract 8 Fructo-oligosaccharide 3 Isomalto-oligosaccharide 5 Total 100

[0064] The preparation of the composition of the present application comprises the following steps: weighing the required amount of flaxseed powder, wolfberry extract, rhizoma polygonati extract, bamboo leaf flavone extract, fructooligosaccharide and isomaltooligosaccharide, uniformly mixing them, and then the product is obtained. The preparation of the flaxseed powder comprises the following steps:

[0065] (1) placing 38 parts of flaxseed oil, 0.02 parts of vitamin E, 2 parts of monoglyceride and 2 parts of sodium stearoyl lactylate into 75 ℃, stirring until uniformly mixed, and then the oil phase is prepared;

[0066] (2) placing 8 parts of flaxseed gum, 7 parts of maltodextrin, 6 parts of sodium caseinate, 6 parts of octenyl succinate starch sodium, 0.2 parts of potassium dihydrogen phosphate, 0.2 parts of sodium tripolyphosphate and 68 parts of water into 75 ℃, stirring until uniformly mixed, and then the water phase is prepared;

[0067] (3) slowly adding the oil phase into the water phase, emulsifying with 2000 rpm high speed shearing machine for 15-30 min, then ultrasonic treating under the condition of 28-30 KHz, 1500-1200 W for 15-30 min, spray drying the prepared emulsion (the inlet air temperature is 185 ℃, the outlet air temperature is 85 ℃), and then the product is obtained. According to the method of the present application, the prepared flaxseed powder contains 28.9% of α-linolenic acid, glucosinolates ≤1.5 ppm, and the average particle size of the flaxseed powder is 161.6 nm.

[0068] Comparative Example Preparation of the flaxseed powder

[0069] The preparation of the flaxseed powder comprises the following steps:

[0070] (1) 38 parts of flaxseed oil, 0.02 parts of vitamin E, 2 parts of monoglyceride and 2 parts of sodium stearoyl lactylate are stirred and mixed uniformly at 60°C, and then kept warm to prepare an oil phase;

[0071] (2) 6 parts of flaxseed gum, 6 parts of malt dextrin, 6 parts of sodium caseinate, 6 parts of sodium octenyl succinate starch, 0.1 part of potassium dihydrogen phosphate, 0.1 part of sodium tripolyphosphate and 70 parts of water are stirred and mixed uniformly at 60°C, and then kept warm to prepare an aqueous phase;

[0072] (3) The oil phase is slowly added to the aqueous phase, and emulsified by a high-speed shearing machine at 1500 rpm for 15-30 min, and then ultrasonically treated at 28-30 kHz and 1000-1200 W for 15-30 min, and then spray dried (the inlet air temperature is 185°C and the outlet air temperature is 85°C) to obtain the product. The flaxseed powder prepared by the method has α-linolenic acid content of 28.3%, cyanogenic glycoside content of ≤5 ppm, and an average particle size of 172.8 nm.

[0073] Test Example 1 Stability test of the composition of the present application

[0074] The composition of Example 1 is placed in a high temperature (60°C) and humidity (RH 75%±10%) environment for 0 day, 7 days, 14 days, 21 days and 28 days, and periodically sampled to observe its appearance and odor. The results show that the powder is uniform and does not form lumps, and has no fishy smell and rancid smell.

[0075] Test Example 2 Study on the improvement of blood rheology by the composition of the present application

[0076] 50 mice with a body weight of 18-22 g, half male and half female, are randomly divided into 5 groups: a blank group (0.5 ml / kg.bw of physiological saline is orally administered), a positive control group (flaxseed powder of the comparative example, 2.6 g / kg.bw), and test groups 1-3 (high, medium and low doses of the composition of Example 1, 2.6 g / kg.bw, 1.3 g / kg.bw and 0.65 g / kg.bw, respectively). 0.5 ml / kg.bw is orally administered every day, once a day, and the oral administration is continuously performed for 10 days. One hour after the last administration, the animals are anesthetized with ether, blood is taken from the abdominal aorta, and heparin is used for anticoagulation, and the following indexes are detected. Blood viscosity: determined by a SWDJ type blood rotational viscometer at a shear rate of 60 s -1 . Plasma viscosity: determined by a capillary viscometer. Red blood cell packed volume: determined by a microcapillary centrifugation method, centrifuged at 15000 r / min for 10 min, and the percentage of red blood cells in the total volume is calculated. Platelet aggregation: determined by a light density method, ADP-induced platelet aggregation is used, and the aggregation peak value is determined by a PA-3210 platelet aggregometer. The results are shown in Table 1.Figure 1 (a)-(d). The results show that the composition of the present application significantly improves the blood rheology.

[0077] Test Example 3 Investigation of the effect of the composition of the present application on improving memory

[0078] Take several ICR mice with a body weight of 18-22 g, after fasting for 12 hours, use the complex maze to select 50 test mice with a feeding time of 100-300 s, half male and half female, and randomly divide them into 5 groups: a blank group (0.5 ml / kg.bw of physiological saline is gavaged), a positive control group (the flaxseed powder of the comparative example, 2.6 g / kg.bw), test groups 1-3 (high, medium and low doses of the composition of Example 1, 2.6 g / kg.bw, 1.3 g / kg.bw and 0.65 g / Kg.bw, respectively). Gavage 0.5 ml / kg.bw once a day, continuously for 10 days. Start training 3 times on the 9th day of gavage, fast for 12 hours, and measure the feeding time 30 minutes after the last administration. The results are shown in Table 2. Figure 2 The results show that the composition of the present application significantly improves the memory of mice.

[0079] Test Example 4 Investigation of the effect of the composition of the present application on improving gastrointestinal motility

[0080] 48 Kunming healthy clean male mice with weight of 18-22 g were selected for small intestine movement test. During the test, the test animals were allowed to freely eat and drink deionized water, and were raised in an environment with room temperature of 20-24℃ and relative humidity of 55-68%. The test animals were randomly divided into four groups, the control group (gavaged with flaxseed powder of the comparative example, the dosage was 2.6 g / kg.bw, dissolved in water), the test group (gavaged with the composition of Example 3, the dosage was 1.3 g / kg.bw, dissolved in water), the blank group (0 mg / kg.bw) and the model group (0 mg / kg.bw) were replaced with water. Each group was given maintenance feed, and gavaged once a day, the gavaging volume was 0.5 ml / kg.bw per day. After the test animals were continuously given the test substance for 14 days, the mice in each group were fasted for 16 h without water, the control group, the test group and the model group were gavaged with loperamide hydrochloride (5.0 mg / Kg.BW), and the blank group was given the corresponding volume of distilled water. 30 min after the test animals were gavaged with loperamide hydrochloride, the test group and the control group were gavaged with ink containing the test substance (containing 5% activated carbon and 10% gum arabic), and the blank group and the model group were given the corresponding volume of ink, the gavaging volume of the test animals was 5 mL / Kg.BW. 30 min after gavage, the test mice were immediately sacrificed by cervical dislocation, the abdominal cavity was opened to separate the mesentery, the upper end from the pylorus to the lower end to the ileocecal junction was cut, the length of the intestinal canal was measured as "total length of small intestine", the distance from the pylorus to the front of ink propulsion was measured as "ink propulsion length", and the ink propulsion rate was calculated according to the following formula. Ink propulsion rate (%) = ink propulsion length (cm) / total length of small intestine (cm) x 100%. The results are shown in Figure 3 .

[0081] The above description of specific embodiments of the present application is not intended to limit the present application, and those skilled in the art can make various changes or modifications to the present application according to the present application, as long as they do not deviate from the spirit of the present application, and all should belong to the scope of protection of the claims of the present application.

Claims

1. A composition for improving blood rheology, the composition consisting of flaxseed meal 65-75 parts, bamboo leaf flavonoid extract 8-12 parts, Rhizoma Polygonati extract 6-8 parts, Fructus Lycii extract 6-8 parts, and prebiotic 5-8 parts, wherein, The flaxseed powder contains 28-32% of alpha-linolenic acid and <2ppm of saponin, the average particle size of the flaxseed powder is 150-200nm, the extract of bamboo leaf flavonoids contains 45-55% of bamboo leaf flavonoids, the extract of polygonatum contains 10-20% of polygonatum polysaccharide, the molar ratio of glucose:mannose:galacturonic acid is 6:25.4:1, the extract of medlar contains 60-80% of total sugar, 1-10% of protein and 1-10% of uronic acid, the molar ratio of mannose: rhamnose: glucose: galactose: arabinose is 1:0.8:5.9:12.3:14.7, and the prebiotic is composed of fructo-oligosaccharide: isomalto-oligosaccharide at a mass ratio of 2-3:2-5. The preparation of the flaxseed powder comprises the following steps: (1) uniformly mix 38-42 parts of flaxseed oil, 0.02-0.03 parts of vitamin E, 2-3 parts of monoglyceride and 2-3 parts of sodium stearoyl lactylate at 65-70°C, and keep warm to prepare an oil phase; (2) uniformly mix 6-8 parts of flaxseed gum, 6-8 parts of malt dextrin, 6-8 parts of sodium caseinate, 6-8 parts of sodium starch octenyl succinate, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of sodium tripolyphosphate and 68-75 parts of water at 65-70°C, and keep warm to prepare an aqueous phase; (3) slowly add the oil phase into the aqueous phase, shear emulsify at 1500-2000rpm for 15-30min, then ultrasonically treat at 28-30KHz and 1000-1200W for 15-30min, and spray dry the prepared emulsion at an inlet temperature of 170-175°C and an outlet temperature of 75-80°C to obtain the product.

2. The composition according to claim 1, wherein the composition comprises 69 parts of flaxseed powder, 10 parts of bamboo leaf flavonoid extract, 8 parts of polygonatum extract, 8 parts of medlar extract, and 5 parts of prebiotic. The prebiotic is composed of fructo-oligosaccharide: isomalto-oligosaccharide at a mass ratio of 1:

1.

3. The composition according to claim 1, wherein the composition comprises 75 parts of flaxseed powder, 8 parts of bamboo leaf flavonoid extract, 6 parts of polygonatum extract, 6 parts of medlar extract, and 5 parts of prebiotic. The prebiotic is composed of fructo-oligosaccharide: isomalto-oligosaccharide at a mass ratio of 2:

3.

4. The composition according to claim 1, wherein the composition comprises 65 parts of flaxseed powder, 12 parts of bamboo leaf flavonoid extract, 7 parts of polygonatum extract, 8 parts of medlar extract, and 8 parts of prebiotics. The prebiotic is composed of fructo-oligosaccharide: isomalto-oligosaccharide at a mass ratio of 3:

5.

5. A preparation method of the composition according to any one of claims 1-4, comprising the following steps: weigh the required amount of flaxseed powder, extract of bamboo leaf flavonoids, extract of polygonatum, extract of medlar and prebiotic, uniformly mix them to obtain the product; The preparation of the flaxseed powder comprises the following steps: (1) uniformly mix 38-42 parts of flaxseed oil, 0.02-0.03 parts of vitamin E, 2-3 parts of monoglyceride and 2-3 parts of sodium stearoyl lactylate at 65-70°C, and keep warm to prepare an oil phase; (2) uniformly mix 6-8 parts of flaxseed gum, 6-8 parts of malt dextrin, 6-8 parts of sodium caseinate, 6-8 parts of sodium starch octenyl succinate, 0.1-0.2 parts of potassium dihydrogen phosphate, 0.1-0.2 parts of sodium tripolyphosphate and 68-75 parts of water at 65-70°C, and keep warm to prepare an aqueous phase; (3) Slowly add the oil phase into the water phase, after shearing emulsification under the condition of 1500-2000 rpm for 15-30 min, then place it under the condition of 28-30 KHz, 1000-1200 W for ultrasonic treatment for 15-30 min, spray dry the prepared emulsion, the inlet air temperature is 170-175 ℃, the outlet air temperature is 75-80 ℃, and then obtain the product.

6. Use of the composition according to any one of claims 1-4 for the preparation of a product for improving blood rheology, improving memory, improving gastrointestinal function, or a combination thereof.

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