A synergistic insecticidal composition for controlling tea stink bugs

Abstract

The present application belongs to the technical field of pest control, and particularly relates to a combined synergistic insecticidal composition for preventing and treating tea fulgorid. The combined synergistic insecticidal composition is composed of baiquloli and bifenthrin, sulfoxaflor or spinetoram, wherein the mass ratio of baiquloli to bifenthrin is 1-40:30-1; the mass ratio of baiquloli to sulfoxaflor is 1:8-1; and the mass ratio of baiquloli to spinetoram is 1-10:20-1. Baiquloli, bifenthrin, sulfoxaflor or spinetoram are reasonably compounded in the present application, compared with single effective component, the prevention and treatment effect on tea fulgorid can be improved, the use amount of pesticide and the prevention and treatment cost of pests can be reduced. In addition, compared with single effective component, the drug resistance risk of pesticide can be reduced, and the pesticide replacement cycle can be prolonged. The combined synergistic insecticidal composition of the present application can provide support for developing pesticides for preventing and treating tea fulgorid.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pest control, and particularly relates to a combined synergistic insecticidal composition for controlling tea stink bugs. BACKGROUND

[0002] Tea stink bug, also known as smelly chestnut bug or smelly plate bug, belongs to the family of Pentatomidae. According to statistics, the host of tea stink bug is more than 300 species, and it is one of the main pests of fruit trees such as peach, pear, apple, plum, apricot and macadamia nut. Of course, it can also harm forest trees, vegetables and other crops. Tea stink bug damages plants by sucking sap from adult and nymph stages, and the damaged fruits are mostly suberized, with hard and bitter flesh, losing economic value. Spraying imidacloprid, bifenthrin, thiamethoxam, flupyradifurone and flonicamid can effectively control tea stink bug. However, with the long-term use of existing pesticides with single component, tea stink bug has developed resistance to them to varying degrees, resulting in reduced control effect. Therefore, it is necessary to develop new pesticides for controlling tea stink bug.

[0003] Patchouli alcohol is a sesquiterpene compound existing in plant volatile oil, CAS: 5986-55-0, molecular formula: C 15 H 26 O, and the structural formula is as follows:

[0004]

[0005] The prior art reports that patchouli alcohol has insecticidal activity. Compounding different pesticide components is an effective and convenient way to develop new pesticides. The inventors found through indoor experiments that patchouli alcohol has a synergistic effect on killing tea stink bug 2nd instar nymphs when compounded with bifenthrin, flonicamid or spinetoram, and no relevant reports on such compounding have been found so far.

[0006] The information disclosed in the background section of this document is intended only to increase an understanding of the general context of the present application and should not be taken as an acknowledgement or any form of suggestion that this information forms prior art that is already known in the art. SUMMARY

[0007] The purpose of the present application is to provide a combined synergistic insecticidal composition for controlling tea stink bugs, which can improve the control effect on tea stink bugs.

[0008] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0009] A synergistic insecticidal composition consisting of ambroin and bifenthrin, sulfoxaflor or spinetoram, wherein the mass ratio of ambroin to bifenthrin is 1-40:30-1; the mass ratio of ambroin to sulfoxaflor is 1:8-1; the mass ratio of ambroin to spinetoram is 1-10:20-1.

[0010] Further, the mass ratio of ambroin to bifenthrin is 1:20.

[0011] Further, the mass ratio of ambroin to sulfoxaflor is 1:4.

[0012] Further, the mass ratio of ambroin to spinetoram is 10:1.

[0013] The synergistic insecticidal composition of the present application is used in the control of evanescent.

[0014] An insecticide prepared from the synergistic insecticidal composition and an auxiliary ingredient.

[0015] Compared with the prior art, the present application has the following beneficial effects:

[0016] The present application reasonably compounds ambroin with bifenthrin, sulfoxaflor or spinetoram, and shows synergistic effect in killing evanescent at a certain mass ratio. Compared with single effective ingredient, the present application can improve the control effect on evanescent, reduce the use amount of pesticide and the control cost of pests. In addition, compared with single effective ingredient, the present application can reduce the risk of drug resistance, prolong the pesticide replacement cycle. The synergistic insecticidal composition of the present application can provide support for the development of pesticides for controlling evanescent. DETAILED DESCRIPTION

[0017] The following clearly and completely describes the technical solutions of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0018] In vitro virulence assays

[0019] 1. Test drug

[0020] 98% spirodiclofen TC (Shanghai Yuan Ye Biotechnology Co., Ltd.), 95% bifenthrin TC (Jiangsu Agricultural Hormone Engineering Technology Research Center Co., Ltd.), 95.9% sulfoxaflor TC (Kedi Hua Agricultural Science and Technology Co., Ltd.), 81.2% spinetoram TC (Kedi Hua Agricultural Science and Technology Co., Ltd.)

[0021] The test agent was completely dissolved in a suitable organic solvent (such as dimethyl sulfoxide), and then diluted with 0.2% Tween-80 to a single-dose stock solution of 10,000 mg / L, which was stored at 4°C for standby use.

[0022] 2. Test insect

[0023] Tea bugs were collected from trees outdoors, and then subcultured in a fresh and clean kidney bean pod in an artificial climate chamber at a temperature of (25±1) °C, light L:D (16h:8h), and relative humidity (60±5)%. Corn was added during the adult stage, and pure water was supplemented. After 6 generations, 2nd instar nymphs with consistent physiological states were selected as the test insect.

[0024] 3. Test method

[0025] The toxicity of spirodiclofen, bifenthrin, sulfoxaflor, spinetoram, and their mixed formulations was determined by the leaf tube drug film method.

[0026] 3.1 Single-agent toxicity determination

[0027] The test agent was diluted with 0.2% Tween-80 to 6 mass concentration gradients. The plastic tube was filled with the drug solution and sealed with a tube cap. After standing for 4h, all the drug solution was poured out, and at this time a uniform drug film was formed on the inner wall of the plastic tube. After drying, a sterile needle tip was used to make multiple small holes on the bottom and cap of the plastic tube. Fresh and clean kidney beans were cut into 2cm long sections, immersed in the drug solution for 5min, then taken out and dried on absorbent paper. One kidney bean section and 10 test insects were placed in each plastic tube. Four replicates were set for each mass concentration of the drug solution, and 0.2% Tween-80 solution was set as a blank control.

[0028] The plastic tube containing the test insects was transferred to an artificial climate chamber at a temperature of (25±1) °C, light L:D (16h:8h), and relative humidity (60±5)%. The survival of the test insects was observed and recorded after 48h. Insects that did not move when touched with a soft brush were considered dead, and the corrected mortality rate of each treatment was calculated. Through linear regression analysis between the corrected mortality rate of the test insects and the logarithmic value of the concentration of the agent, the toxicity regression equation and LC 50 values of each single agent on the test insect were obtained.

[0029] 3.2 Mixed-agent joint toxicity determination

[0030] On the basis of single agent toxicity determination, baiquli alcohol was set with bifenthrin, sulfoxaflor or spinetoram in multiple groups of different proportions, and each group of mixed agent was diluted into 6 mass concentration gradients with 0.2% volume fraction of Tween-80. The above drugs were prepared and used immediately. The corrected mortality of test pests of mixed agent was determined by referring to the method of single agent toxicity determination in 3.1. Through linear regression analysis between the corrected mortality of test pests and the logarithm value of the concentration of the agent, the toxicity regression equation and LC 50 value of each mixed agent to test pests were obtained.

[0031] 4. Data statistics

[0032] The synergistic effect of the agent was evaluated according to the calculated synergistic coefficient (CTC) of the mixed agent (CTC). When CTC≤80, it was antagonistic effect; when 80<CTC<120, it was additive effect; and when CTC≥120, it was synergistic effect.

[0033]

[0034] In the above formula: P--mortality, unit is %; K--number of dead insects; N--total number of treated insects.

[0035]

[0036] In the above formula: P1--corrected mortality, unit is %; P t --treatment mortality, unit is %; P0--blank control mortality, unit is %.

[0037] 5. Agent evaluation

[0038] See Tables 1-3.

[0039] Table 1 Toxicity determination results of single agent on tea stink bugs

[0040]

[0041] From Table 1, the LC 50 values of baiquli alcohol, bifenthrin, sulfoxaflor and spinetoram on tea stink bugs were 217.8507 mg / L, 3.4403 mg / L, 16.1735 mg / L and 10.0133 mg / L, respectively.

[0042] Table 2 Toxicity determination results of baiquli alcohol and bifenthrin in different proportions on tea stink bugs

[0043]

[0044] From Table 2, it can be seen that the co-toxicity coefficients of baiquli alcohol and bifenthrin to Eurydema dominulus are all greater than 120 when the mass ratio of baiquli alcohol to bifenthrin is in the range of 1-40:30-1, that is, the bioactivity of baiquli alcohol and bifenthrin to Eurydema dominulus shows a synergistic effect after being compounded.

[0045] Table 3 Toxicity determination results of baiquli alcohol and sulfoxaflor in different proportions to Eurydema dominulus

[0046]

[0047]

[0048] From Table 3, it can be seen that the co-toxicity coefficients of baiquli alcohol and sulfoxaflor to Eurydema dominulus are all greater than 120 when the mass ratio of baiquli alcohol to sulfoxaflor is in the range of 1:8-1, that is, the bioactivity of baiquli alcohol and sulfoxaflor to Eurydema dominulus shows a synergistic effect after being compounded.

[0049] Table 4 Toxicity determination results of baiquli alcohol and spinetoram in different proportions to Eurydema dominulus

[0050]

[0051] From Table 4, it can be seen that the co-toxicity coefficients of baiquli alcohol and spinetoram to Eurydema dominulus are all greater than 120 when the mass ratio of baiquli alcohol to spinetoram is in the range of 1-10:20-1, that is, the bioactivity of baiquli alcohol and spinetoram to Eurydema dominulus shows a synergistic effect after being compounded.

[0052] The foregoing description of specific exemplary embodiments of the application is intended to illustrate and exemplify the application. These descriptions are not meant to limit the application to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teachings. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application to thereby enable others skilled in the art to best utilize the application and various embodiments with various modifications as are suited to the particular use contemplated. It is intended that the scope of the application be defined by the claims and their equivalents.

Claims

1. A synergistic insecticidal composition, characterized in that, The synergistic insecticidal composition is composed of centaurea solstitialis alcohol and bifenthrin, wherein the mass ratio of the centaurea solstitialis alcohol to the bifenthrin is 1-40:30-1.

2. The synergistic insecticidal composition according to claim 1, wherein The mass ratio of the centaurea solstitialis alcohol to the bifenthrin is 1:20.

Images